FastQCFastQC Report
Fri 17 Jun 2016
SRR1526185_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526185_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences43608
Sequences flagged as poor quality0
Sequence length56
%GC35

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGTTTGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTT1900.4356998715831957No Hit
GGGTTTGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1110.2545404512933407No Hit
GGGTTTGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA770.17657310585213723No Hit
GGGTTTGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACAAAAAAAAAAAAAAA630.14446890478811228No Hit
GGGTTTGGGGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT620.14217574756925333No Hit
GGGTTTGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTCTTTTTTTTTTTTTTT580.13300311869381765No Hit
GGGTTTGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA530.12153733259952303No Hit
GGGTTTGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAACCAAAAAAAAAAAAAAA510.11695101816180517No Hit
GGGTTTGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACAAAAAAAAAAAAAAA480.1100715465052284No Hit
GGGTTTGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA470.10777838928636947No Hit
GGGTTTGGGGCCGAATAGCCTCTCCACCCAAGCGGCCGGAGAACCTGCGTGCAATC450.10319207484865162No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAACACC150.00816800950.31981738
CCGTCTC150.00816800950.31981735
TCTCCTG150.00816800950.31981738
AAAAACT150.00816800950.31981735
CCTGCTT150.00820554750.26157841
CTCCTGC150.00820554750.26157839
AGAACCT150.00820554750.26157839
TCCTGCT150.00820554750.26157840
AACACCA150.00820554750.26157839
AACCAAC204.0337906E-450.26157441
CCAACAC252.0052647E-550.2034743
CCAAAAC600.050.2034743
TGATATG150.00831893250.0876650
GCAATCC150.00831893250.0876650
AATAGCC150.00831893250.0876614
AATGGTA355.1088136E-850.02995710
AGCATGT550.050.02995711
GAGCGCA150.00835698550.02995311
AACTGTA150.00835698550.02995310
AAGAGTG150.00835698550.02995310