##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1526185_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 43608 Sequences flagged as poor quality 0 Sequence length 56 %GC 35 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 38.20434323977252 39.0 38.0 40.0 35.0 40.0 2 37.9302650889745 39.0 38.0 40.0 35.0 40.0 3 37.82069803705742 39.0 38.0 40.0 35.0 40.0 4 36.822257383966246 39.0 36.0 39.0 33.0 40.0 5 38.05856723536966 39.0 38.0 40.0 35.0 40.0 6 38.064139607411484 39.0 38.0 40.0 35.0 40.0 7 37.99715648504861 39.0 38.0 40.0 35.0 40.0 8 37.88508989176298 39.0 38.0 40.0 35.0 40.0 9 37.90657677490369 39.0 38.0 40.0 35.0 40.0 10 37.414235920014676 39.0 38.0 40.0 33.0 40.0 11 36.427215189873415 38.0 36.0 40.0 31.0 40.0 12 34.4672537149147 38.0 33.0 39.0 22.0 40.0 13 34.15987892129884 38.0 33.0 39.0 21.0 40.0 14 34.314070812694915 38.0 33.0 39.0 22.0 40.0 15 34.174371674922035 38.0 33.0 39.0 21.0 40.0 16 33.35264171711613 38.0 31.0 39.0 20.0 40.0 17 33.39884883507613 37.0 31.0 39.0 20.0 40.0 18 32.71739130434783 37.0 30.0 39.0 17.0 40.0 19 33.59961474958723 37.0 31.0 39.0 22.0 40.0 20 33.86241056686846 37.0 31.0 39.0 23.0 40.0 21 34.61500183452578 38.0 33.0 39.0 25.0 40.0 22 34.41224087323427 38.0 33.0 39.0 23.0 40.0 23 34.75933314988075 38.0 33.0 39.0 26.0 40.0 24 34.325307283067325 38.0 33.0 39.0 23.0 40.0 25 34.55533388369106 38.0 33.0 39.0 25.0 40.0 26 35.42927903137039 38.0 35.0 39.0 28.0 40.0 27 35.32166116308934 38.0 35.0 39.0 27.0 40.0 28 35.40742524307466 38.0 35.0 39.0 28.0 40.0 29 35.15643918547055 38.0 35.0 39.0 27.0 40.0 30 35.188979086406164 38.0 35.0 39.0 27.0 40.0 31 35.63277380297193 38.0 35.0 39.0 29.0 40.0 32 35.21849201981288 38.0 35.0 39.0 27.0 40.0 33 35.4676435516419 38.0 35.0 39.0 29.0 40.0 34 35.55427903137039 38.0 35.0 40.0 29.0 40.0 35 35.3320033021464 38.0 35.0 39.0 28.0 40.0 36 34.311662997615116 38.0 34.0 39.0 27.0 40.0 37 34.08865345808108 38.0 33.0 39.0 26.0 40.0 38 33.846266740047696 38.0 33.0 39.0 25.0 40.0 39 33.28001742799486 37.0 32.0 39.0 20.0 40.0 40 30.0731517152816 35.0 26.0 39.0 7.0 40.0 41 26.363511282333516 32.0 14.0 38.0 4.0 40.0 42 30.922055586130984 35.0 29.0 38.0 4.0 40.0 43 29.557283067327095 35.0 27.0 38.0 4.0 40.0 44 29.443496606127315 35.0 26.0 38.0 4.0 40.0 45 30.549279948633277 35.0 29.0 38.0 4.0 40.0 46 30.384516602458266 35.0 28.0 39.0 4.0 40.0 47 29.86848743349844 35.0 27.0 39.0 4.0 40.0 48 29.938497523390204 35.0 27.0 39.0 4.0 40.0 49 27.349706475875987 35.0 15.0 38.0 4.0 40.0 50 29.421757475692534 35.0 27.0 39.0 4.0 40.0 51 29.409420289855074 35.0 27.0 39.0 4.0 40.0 52 29.236722619702807 35.0 26.0 39.0 4.0 40.0 53 28.838148963492937 35.0 24.0 39.0 4.0 40.0 54 27.76155751238305 35.0 16.0 39.0 4.0 40.0 55 27.961062190423775 35.0 17.0 39.0 4.0 40.0 56 26.973880939277198 35.0 16.0 39.0 4.0 40.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1 1 0.20183209215834097 1 2 0.5582609746981859 1 3 0.783177474488788 1 4 0.3180132639984947 1 5 0.5519809629024977 1 6 0.573696122218756 1 7 0.7137935135401534 1 8 0.6893772115640928 1 9 0.65733811887673 1 10 0.7571273000422423 1 11 -2.1785164176693 1 12 -12.995371817392083 1 13 -8.924553610138183 1 14 -5.212771075799665 1 15 -7.106645365687076 1 16 -22.851381584337723 1 17 -5.590702420363176 1 18 -14.375576339640684 1 19 -5.676831220235414 1 20 -5.5550567542852995 1 21 -5.461362599626202 1 22 -3.108249793220221 1 23 -2.95902379370283 1 24 -4.0996206469137775 1 25 -2.6687886878547964 1 26 2.8469498321706794 1 27 2.6945189938323395 1 28 2.2019783010598815 1 29 2.938109308799376 1 30 2.54931717954382 1 31 2.4387481149601697 1 32 2.3845169212427635 1 33 2.045635227777943 1 34 1.9963056034345215 1 35 1.753880409582898 1 36 2.843116358616193 1 37 3.076827166047565 1 38 3.5148100155508075 1 39 3.8269476562355607 1 40 7.903821151415691 1 41 -11.330629627327573 1 42 -12.878665264461578 1 43 -17.40851747434471 1 44 -22.304877450189487 1 45 -13.262252653383136 1 46 -15.769629110520883 1 47 -23.80972189346744 1 48 -15.07642670797069 1 49 -19.69152061510057 1 50 -14.408727179651649 1 51 -12.48420042196944 1 52 -12.500852440276223 1 53 -12.468873709827758 1 54 -11.419010968046715 1 55 -11.585226049399811 1 56 -6.869825712706405 2 1 0.7951942855205303 2 2 1.0018396182768257 2 3 1.2720663633776752 2 4 1.234030580015805 2 5 1.0679982789198093 2 6 1.2553700038926365 2 7 1.0912826910293347 2 8 1.083028005214885 2 9 1.116789778328389 2 10 1.0858430287579708 2 11 1.427544188391309 2 12 -2.8903213123415767 2 13 -1.5066603922449673 2 14 2.823015359986769 2 15 2.698260839219131 2 16 1.7777670448109077 2 17 3.210451980791227 2 18 -1.8592704233347668 2 19 1.8787243353201433 2 20 2.550570951342408 2 21 2.927959189695585 2 22 2.8346073496369257 2 23 2.741697707018673 2 24 1.423323076029945 2 25 2.480706261640151 2 26 2.6212644064852597 2 27 2.4512289505423013 2 28 2.4672019662835467 2 29 2.345324316014377 2 30 2.4765899068165496 2 31 2.072225748437809 2 32 1.495339432065279 2 33 1.2632398453825573 2 34 1.0958727030016178 2 35 1.9273291830316737 2 36 3.0711105866104234 2 37 3.36398445320485 2 38 3.0305387312795276 2 39 4.071969301257205 2 40 7.622147269741809 2 41 -2.7871952838932295 2 42 -5.436241022037336 2 43 -9.354549220376455 2 44 -14.183088128400167 2 45 -5.321704312834797 2 46 -9.170206311098084 2 47 -18.99644628019183 2 48 -6.949442580986563 2 49 -11.870164193744147 2 50 -6.49068966161413 2 51 -1.2103187480877686 2 52 -1.5259606653844493 2 53 -0.996723637677686 2 54 0.07391832488257677 2 55 -0.029093293267056453 2 56 4.59453215165146 3 1 0.6624381527644019 3 2 0.5855337019709097 3 3 1.1710562623675784 3 4 1.599831445816676 3 5 1.0398597507812823 3 6 0.5858173343399642 3 7 0.4440965438431874 3 8 0.9257408479277274 3 9 0.12400478554339855 3 10 0.4328848757998145 3 11 1.7608775217246375 3 12 4.316749394729129 3 13 2.7148403292557575 3 14 3.2963198332912427 3 15 3.538809179767469 3 16 4.542557809601668 3 17 4.869903640242882 3 18 5.027453963389618 3 19 4.126199082794891 3 20 3.4964583972298513 3 21 2.8204555821919826 3 22 3.5856896007191708 3 23 2.907642872963841 3 24 3.0640157167225865 3 25 2.955453736387625 3 26 2.3348286200494712 3 27 2.812700812014157 3 28 2.5595540586356336 3 29 2.89871536940543 3 30 2.597802028028674 3 31 2.0599602361722944 3 32 2.6360320727579136 3 33 1.948665530808242 3 34 2.32963893676785 3 35 2.1993349550374433 3 36 3.6764496919495286 3 37 3.8040998933202914 3 38 4.314810015550812 3 39 4.836038565326469 3 40 8.867457515052052 3 41 9.11179461509667 3 42 4.897092311295996 3 43 -0.532759898587134 3 44 1.6466377013256626 3 45 4.428656437525952 3 46 2.1667345258427524 3 47 -2.1248734086189565 3 48 2.802361170817189 3 49 4.61757029399034 3 50 1.764000093075623 3 51 2.8127692750002566 3 52 2.5476324082086244 3 53 2.5674899265358775 3 54 3.8264435774078294 3 55 2.502652738478975 3 56 4.639265196384503 4 1 0.038738469363224226 4 2 -0.42057213249313463 4 3 -0.31831506282464517 4 4 -0.58402201415619 4 5 -0.4515468525656203 4 6 -0.24095788049494615 4 7 -0.42541951224003327 4 8 -0.367339206346351 4 9 -0.3065696151802584 4 10 -0.32401245572437887 4 11 1.1240616013266305 4 12 3.854288969582136 4 13 3.097698764352998 4 14 2.5242709866155337 4 15 2.9316178636209287 4 16 4.200178795580868 4 17 4.136298936488927 4 18 4.792944691566916 4 19 3.4873478842421974 4 20 2.996910681264225 4 21 1.960889774864981 4 22 2.4230934903618717 4 23 1.5367699647774984 4 24 2.787217887685948 4 25 2.198782546880615 4 26 1.5584830750472065 4 27 1.5677890074008616 4 28 1.3894952617111684 4 29 1.9826140577817313 4 30 1.416888414279235 4 31 1.354623284566685 4 32 1.8841098656118263 4 33 1.367480546638184 4 34 1.1889786020776683 4 35 0.9612074109397497 4 36 2.806481351831934 4 37 3.0116982650977633 4 38 2.7869945474368336 4 39 4.045672215258627 4 40 6.969221422786113 4 41 9.577647170501464 4 42 5.752813704330823 4 43 6.30193028684932 4 44 6.508691070841717 4 45 4.9374759761962395 4 46 5.0832881215008285 4 47 4.710495202869058 4 48 5.475812097999459 4 49 7.765467173230501 4 50 5.490594394296789 4 51 5.05881178969949 4 52 4.904032227295012 4 53 4.420949899398838 4 54 5.903105721234152 4 55 5.400662688727735 4 56 7.673186498962526 5 1 0.43011492044117006 5 2 0.6685062849434971 5 3 0.7323838236951374 5 4 0.9324432784285079 5 5 0.8299030408245756 5 6 0.8426715911942253 5 7 0.8373144370610817 5 8 1.0354089575958412 5 9 0.7834564449950534 5 10 0.48266842558336265 5 11 1.887861648708764 5 12 4.077932655912392 5 13 3.4933396077550327 5 14 4.616666153637567 5 15 4.190324331282625 5 16 4.380941647985509 5 17 4.591404361743606 5 18 5.489935925871585 5 19 4.164438621034428 5 20 3.4870788878503447 5 21 3.1501814119178064 5 22 3.8187343337639064 5 23 2.789316754637717 5 24 2.470942123648989 5 25 3.083880864814752 5 26 1.4466612318820822 5 27 1.5464670457803962 5 28 1.181487680569262 5 29 1.3611973318873964 5 30 1.2861137163403598 5 31 -0.023012346800285854 5 32 0.5112124479382913 5 33 -0.5780299958872845 5 34 0.3180949252238392 5 35 -0.7393374836349906 5 36 1.055237570737404 5 37 1.3957304849508816 5 38 1.3003800011207929 5 39 1.9449851742730786 5 40 4.876115523710062 5 41 7.657249160551217 5 42 4.405028819232509 5 43 5.518466652639418 5 44 5.515324570012535 5 45 5.122740131609646 5 46 3.893285752393979 5 47 3.9400616563161073 5 48 3.955319323775342 5 49 6.479042155462199 5 50 4.334707163782692 5 51 1.6150780773090574 5 52 3.0613409219171395 5 53 2.574704933750887 5 54 3.2485214994857508 5 55 1.2248749607012002 5 56 2.9913575484768558 6 1 0.23170222202846702 6 2 -0.1449857785485662 6 3 -0.08110823979692583 6 4 0.0852210562062865 6 5 -0.18795410203257035 6 6 0.29902079754342736 6 7 0.15080650055314493 6 8 0.36080578299266364 6 9 -0.7601943486557374 6 10 -0.43995062203568125 6 11 0.7549251407722579 6 12 3.1176151955949294 6 13 3.3306411950566215 6 14 3.569047106018516 6 15 3.106990997949289 6 16 3.7995924416362996 6 17 2.736245631584879 6 18 3.9046184655541225 6 19 3.341025922621732 6 20 2.562475713247167 6 21 2.0152607769971738 6 22 2.6024644924940645 6 23 2.255586595907559 6 24 2.589989742696609 6 25 2.0263411822750754 6 26 0.19467710489795564 6 27 0.2964670457803962 6 28 0.6477575218390967 6 29 0.5377846334747005 6 30 0.9765899068165496 6 31 -0.5904726642606093 6 32 0.3286727653986077 6 33 0.044985877128588925 6 34 -1.237460630331718 6 35 -0.4694962137937253 6 36 0.2992851897850244 6 37 1.1774765166969132 6 38 0.7190307947715908 6 39 2.2207788250667306 6 40 5.507067904662442 6 41 1.734630112932166 6 42 3.5875685017721857 6 43 4.820053954226722 6 44 4.27921345890142 6 45 3.4838512427207604 6 46 3.0401111492193778 6 47 2.7198235610780124 6 48 2.905716149172168 6 49 4.580232631652677 6 50 3.338675417750949 6 51 2.1369034741344564 6 52 2.2657060012822186 6 53 1.2314509654969186 6 54 0.712807213771466 6 55 0.990747976574216 6 56 2.4477067548260614 7 1 0.5061383122540306 7 2 0.31679367007298254 7 3 0.6814230885238786 7 4 0.7904215574594176 7 5 0.7998278528546479 7 6 0.8936323263654842 7 7 0.6602049967937447 7 8 0.4823596677044435 7 9 0.8369234458304788 7 10 0.2646233128014117 7 11 2.6907020831281514 7 12 4.244181611635028 7 13 4.226004603577927 7 14 4.533959386720269 7 15 4.541201524265077 7 16 5.848777905295449 7 17 5.349967436096151 7 18 4.929367838987709 7 19 4.481063516606689 7 20 4.447814059111828 7 21 3.1418271930372725 7 22 3.9022765225692524 7 23 3.3398597788649553 7 24 4.062420820390848 7 25 3.276027899067053 7 26 2.442484122441819 7 27 2.7544870959057093 7 28 1.9901760682050167 7 29 2.8708046836000136 7 30 3.687116222606022 7 31 2.6620336014035573 7 32 1.6782968255489834 7 33 2.114534749309044 7 34 1.8176772142798114 7 35 2.4377719566323393 7 36 2.7920796760005615 7 37 2.362313609428739 7 38 3.1616999677039175 7 39 3.8990369704301386 7 40 7.3873937191987835 7 41 1.4642667044108642 7 42 5.142706346383715 7 43 5.812535157234237 7 44 6.141055564164578 7 45 4.8353550021192575 7 46 3.8740710489687515 7 47 3.932542859323629 7 48 4.941117151678434 7 49 7.158240150449668 7 50 5.077397222262224 7 51 5.0870914440592685 7 52 3.759753620329839 7 53 3.1879045995821294 7 54 4.892631775174976 7 55 5.1079158963737115 7 56 5.631290714725811 8 1 0.1457458055635783 8 2 0.5323749962698372 8 3 0.9086012033023465 8 4 1.4850394581433335 8 5 0.45460037738632053 8 6 0.5986576014175213 8 7 0.6102495998267514 8 8 0.6453094149539282 8 9 0.5467063777365126 8 10 1.0258484094413163 8 11 2.636583736413904 8 12 4.179358536999288 8 13 4.55279615873701 8 14 4.687691173815125 8 15 3.8230926928645417 8 16 5.1116384464789135 8 17 4.989574929405698 8 18 5.349231056353155 8 19 4.34011793230696 8 20 4.2640495631261 8 21 3.1956481861981416 8 22 3.5900552915255446 8 23 2.7032258210891555 8 24 3.2982221882171956 8 25 3.241534886875556 8 26 2.302727952355582 8 27 2.6917454961435894 8 28 2.5893434782555644 8 29 2.316537660109077 8 30 2.510488211901297 8 31 0.9379776989355193 8 32 1.867413685495464 8 33 1.2126614219227818 8 34 1.549464309135594 8 35 1.770213229305547 8 36 1.490265819324975 8 37 1.357258599021364 8 38 1.6969363637788533 8 39 1.728950737899659 8 40 5.058520689166073 8 41 -1.8286265456634752 8 42 3.501612085307297 8 43 4.713516423960378 8 44 3.777094814833621 8 45 2.4687180708079275 8 46 3.253791536628576 8 47 3.7261794932814034 8 48 3.6015393937242237 8 49 6.441915440369389 8 50 2.886496240995502 8 51 3.6366008106962013 8 52 3.8670982530981988 8 53 3.6815114981845696 8 54 4.16619704427994 8 55 4.126946523789712 8 56 4.047585689450759 9 1 0.08884507917132822 9 2 0.5514427928800032 9 3 -0.5275368112254952 9 4 0.2995067704920018 9 5 0.044188755110290856 9 6 0.44187794040057327 9 7 0.15080650055314493 9 8 -0.06776564557875986 9 9 0.09694850848711667 9 10 -0.6363791934642506 9 11 1.3084965693436885 9 12 3.29618662416636 9 13 3.241355480770906 9 14 3.9976185345899466 9 15 2.874848140806435 9 16 4.085306727350584 9 17 4.111245631584879 9 18 4.886761322696977 9 19 3.4124544940503014 9 20 3.0446185703900284 9 21 2.3188322055686044 9 22 2.3881787782083492 9 23 1.8448723101932742 9 24 2.64356117126804 9 25 1.3120554679893601 9 26 0.783962819183671 9 27 1.2607527600661044 9 28 -0.29867104958947266 9 29 -0.01578679509673009 9 30 0.9587327639594037 9 31 -1.108329807117748 9 32 -0.09989866317281582 9 33 -1.4728712657285499 9 34 -1.362460630331718 9 35 -1.0944962137937253 9 36 -0.9150005245006909 9 37 -1.1975234833030868 9 38 -0.5309692052284092 9 39 -0.13636403207612702 9 40 3.4892107618052997 9 41 2.145344398646454 9 42 2.9625685017721857 9 43 4.070053954226722 9 44 2.564927744615705 9 45 2.751708385577899 9 46 2.05796829207652 9 47 2.7912521325065853 9 48 1.9235732920293103 9 49 4.812375488795535 9 50 3.427961132036664 9 51 3.047617759848741 9 52 2.7299917155679303 9 53 2.249308108354061 9 54 2.7485214994857508 9 55 2.419319405145643 9 56 3.2155638976832037 10 1 0.5349367233761768 10 2 0.8368201513705671 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25 2.054780335714227 116 26 1.8011585863794366 116 27 1.9591654584788074 116 28 0.7767257758073569 116 29 1.6310386017286689 116 30 1.4951084253350686 116 31 1.1171992934113533 116 32 0.8498367865626335 116 33 0.7129752951180066 116 34 1.2387298458587637 116 35 1.3929376486401353 116 36 1.7589412744411135 116 37 3.0094870987074955 116 38 2.1919143926551854 116 39 3.6037153330032368 116 40 7.685639333233873 116 41 3.0461380494401027 116 42 -1.8806854664817791 116 43 -10.07148043730767 116 44 -10.07726802258006 116 45 -1.4248789160093978 116 46 -3.813063453955227 116 47 -3.35888014262569 116 48 -4.372723004266987 116 49 -11.010375833955786 116 50 -4.294922465846934 116 51 -3.6283081660771863 116 52 -4.1846908241146075 116 53 -2.787728928682977 116 54 -8.592748341784091 116 55 -2.1269768911506546 116 56 -0.05097049385118524 117 1 -0.6397263494000995 117 2 -0.4235572071199982 117 3 -0.18825109693977993 117 4 -0.37549322950800246 117 5 -0.5415255306039981 117 6 -0.40812205959942816 117 7 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1.4036099029232503 118 28 1.293819792901374 118 29 1.186594157284219 118 30 1.5278719580986007 118 31 0.2895639657760256 118 32 -5.794037857312013 118 33 0.6759382580809685 118 34 0.5977042048331214 118 35 0.2960715517740411 118 36 1.593699109198944 118 37 1.8413959306163292 118 38 1.6178403185811092 118 39 2.8558520851399862 118 40 5.762562410156953 118 41 3.8623773656794214 118 42 -0.6670102528065662 118 43 -10.352107217934453 118 44 -9.13424807956012 118 45 -2.6086395997700826 118 46 -8.834430975322746 118 47 -11.052612336357885 118 48 -10.512324143868124 118 49 -13.575902899482852 118 50 -6.605463776388245 118 51 -5.189561727330744 118 52 -5.72742586684965 118 53 -5.558384199338246 118 54 -12.165397914433662 118 55 -5.125552389726153 118 56 -2.70339214627284 119 1 -0.7068359839515992 119 2 -0.428208369910692 119 3 -0.4772876417902836 119 4 -0.5323038607372368 119 5 -0.5588012781123055 119 6 -0.6220755479715194 119 7 -0.5576652602441996 119 8 -0.6873669744824156 119 9 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29 0.6136774906175546 120 30 0.8828399068165496 120 31 -0.3851155214034634 120 32 -11.149005806029962 120 33 0.07177159141430423 120 34 -0.46960348747457203 120 35 -0.44271049950801 120 36 -0.11142909592926031 120 37 -0.9430591975888021 120 38 -0.5175763480855551 120 39 0.5377431107810153 120 40 3.5293893332338726 120 41 -0.33233417278211874 120 42 -4.613324355370668 120 43 -15.296017474344708 120 44 -14.06453654109858 120 45 -8.230434471564955 120 46 -17.924174565066338 120 47 -16.811426438921988 120 48 -9.48267670797069 120 49 -18.06708879691875 120 50 -10.772931725106194 120 51 -8.671132240151259 120 52 -8.917329713003497 120 53 -8.344441891645939 120 54 -16.037192786228534 120 55 -8.455680594854357 120 56 -5.494257530888223 >>END_MODULE >>Per sequence quality scores pass #Quality Count 4 68.0 5 17.0 6 22.0 7 27.0 8 76.0 9 149.0 10 94.0 11 85.0 12 72.0 13 45.0 14 95.0 15 105.0 16 137.0 17 175.0 18 233.0 19 286.0 20 355.0 21 397.0 22 499.0 23 607.0 24 740.0 25 793.0 26 858.0 27 959.0 28 1109.0 29 1319.0 30 1628.0 31 1954.0 32 2237.0 33 2483.0 34 2885.0 35 3464.0 36 4254.0 37 5464.0 38 5653.0 39 4250.0 40 14.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 100.0 0.0 0.0 0.0 2 100.0 0.0 0.0 0.0 3 100.0 0.0 0.0 0.0 4 0.0 0.0 100.0 0.0 5 0.0 0.0 100.0 0.0 6 0.0 0.0 100.0 0.0 7 99.25243074665198 0.28893780957622456 0.38983672720601725 0.06879471656576776 8 99.20427444505594 0.49073564483580995 0.23160887910475145 0.0733810310034856 9 96.74371674922033 1.949183636030086 0.5985140341221794 0.7085855806274078 10 21.46165841130068 57.41607044578977 10.592093193909374 10.530177949000183 11 23.115024766097964 45.76912493120528 16.818015043111355 14.297835259585398 12 43.34296459365254 20.911300678774538 16.033755274261605 19.71197945331132 13 25.580275229357795 18.924311926605505 21.956422018348622 33.538990825688074 14 26.718800165114892 28.725404760812733 21.370912259780763 23.184882814291612 15 32.17077432407578 21.305407393783337 21.72153761265404 24.802280669486848 16 25.799862290566907 27.082855175579528 25.753959146201517 21.363323387652052 17 30.255916345624655 27.038616767565582 21.24610163272794 21.45936525408182 18 27.156190519870666 29.01827688215195 21.189258605269796 22.636273992707594 19 23.83499380193747 33.694504384555344 19.67770074835866 22.792801065148524 20 24.30552366958997 33.100693328435646 19.72542357316681 22.868359428807565 21 25.399009356081454 26.66483214089158 24.571179600073382 23.364978902953588 22 31.932214272610533 26.15345808108604 17.409649605576956 24.50467804072647 23 30.36369473491102 31.468996514401027 21.16584113006788 17.00146762062007 24 26.83575654726414 30.775581342017155 24.826858689171218 17.56180342154749 25 23.789758525007453 29.809434265140915 30.233678079207465 16.167129130644163 26 23.648958620056888 28.066795118818238 35.2348839343059 13.049362326818976 27 23.30839147998531 24.777359529930223 34.91782959970621 16.996419390378257 28 24.89267430381781 27.45012511765651 34.44523519823687 13.211965380288804 29 23.225347588675263 26.34332124994264 36.65167714403707 13.779654017345019 30 16.90638405248549 31.40183056912807 37.97857454178423 13.713210836602208 31 14.564643799472297 32.94252609842836 41.84008259722381 10.65274750487553 32 9.953161592505854 32.63305322128851 46.93943151030904 10.47435367589659 33 9.421305336672104 33.09288060833008 48.128833651129135 9.356980403868684 34 9.099880657302856 32.091710272652165 50.755072064628656 8.053337005416322 35 8.22967824849681 34.02258227383302 50.87666957359893 6.871069904071235 36 7.770332293005324 32.95621442996144 51.457224160088124 7.816229116945108 37 7.902176661226928 32.56487461787758 53.2144252648999 6.318523455995587 38 9.316127251397418 32.98829158328157 52.059899247808985 5.635681917512019 39 8.566176470588236 32.626378676470594 51.65211397058823 7.155330882352941 40 6.530376530376531 26.153846153846157 47.124047124047124 20.19173019173019 41 6.65016081634542 23.180692782932642 44.2025129000162 25.96663350070574 42 7.862328830070766 34.08923812149619 52.83291976840364 5.215513280029409 43 7.424103035878566 29.420423183072675 50.94526218951242 12.210211591536337 44 7.002904430408925 30.55184177769582 49.684200820616844 12.761052971278412 45 7.140886518300533 34.768714364539264 52.52666911899945 5.563729998160751 46 7.173393398915143 34.35460145260642 53.02013422818792 5.451870920290522 47 8.244040107381318 32.698529243053486 51.95603790468761 7.1013927448775895 48 8.707742513731528 34.323075862385956 51.65352883046446 5.315652793418059 49 7.615664929194151 28.243922690160982 45.265002998293284 18.875409382351584 50 7.321555744446232 35.787635828987575 51.19115991637758 5.699648510188609 51 7.899399433949239 36.80480452840608 49.6030741618537 5.692721875790975 52 7.1275984839784075 37.40668427701849 49.360284828299065 6.105432410704031 53 5.989703043118507 38.97444148202629 49.17256596488002 5.863289509975177 54 6.370012648039554 36.3113717373807 46.5884787857882 10.730136828791537 55 7.451187820527562 38.159740588275874 49.182439114136564 5.20663247706 56 7.472876057374036 38.869988966531814 48.68977565281353 4.967359323280618 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 1.5 5 3.0 6 6.0 7 9.0 8 6.5 9 4.0 10 47.0 11 90.0 12 189.5 13 360.0 14 431.0 15 465.5 16 500.0 17 541.5 18 583.0 19 695.5 20 808.0 21 895.0 22 1203.5 23 1425.0 24 1811.0 25 2197.0 26 2702.5 27 3208.0 28 3565.5 29 4100.0 30 4277.0 31 4324.0 32 4371.0 33 3921.5 34 3472.0 35 3095.5 36 2719.0 37 2223.0 38 1404.5 39 1082.0 40 986.5 41 891.0 42 816.5 43 742.0 44 681.5 45 621.0 46 633.0 47 642.0 48 639.0 49 664.0 50 689.0 51 758.0 52 827.0 53 957.0 54 1152.5 55 1218.0 56 1208.0 57 1198.0 58 1076.5 59 955.0 60 794.5 61 634.0 62 543.0 63 423.5 64 395.0 65 312.0 66 229.0 67 173.5 68 118.0 69 98.0 70 78.0 71 60.5 72 34.0 73 25.0 74 20.5 75 16.0 76 9.5 77 3.0 78 2.0 79 1.0 80 1.0 81 1.0 82 1.0 83 0.5 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0183452577508714 14 0.00458631443771785 15 0.2568336085121996 16 0.08713997431663915 17 0.0 18 0.002293157218858925 19 0.10548523206751054 20 0.11465786094294625 21 0.0 22 0.0 23 0.0 24 0.00458631443771785 25 0.002293157218858925 26 0.0275178866263071 27 0.091726288754357 28 0.11236470372408733 29 0.050449458814896354 30 0.03439735828288388 31 0.05274261603375527 32 0.12383048981838193 33 0.18115942028985507 34 0.0825536598789213 35 0.07796734544120346 36 0.0733810310034856 37 0.23160887910475145 38 0.30957622454595485 39 0.20179783525958542 40 0.7292239955971381 41 0.8966244725738396 42 0.1926252063841497 43 0.2935241240139424 44 0.5182535314621171 45 0.2568336085121996 46 0.2293157218858925 47 0.057328930471473125 48 0.21784993579159784 49 0.5732893047147313 50 0.18115942028985507 51 0.34168042560997985 52 0.16740047697670152 53 0.2293157218858925 54 0.2820583379196478 55 0.28664465235736564 56 0.2384883507613282 >>END_MODULE >>Sequence Length Distribution pass #Length Count 56 43608.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 75.21555677857273 #Duplication Level Percentage of deduplicated Percentage of total 1 88.6189024390244 66.65520088057238 2 6.173780487804878 9.287286736378645 3 2.0365853658536586 4.595487066593286 4 0.9878048780487805 2.971931755641167 5 0.524390243902439 1.9721152082186757 6 0.3323170731707317 1.4997248211337368 7 0.3201219512195122 1.68547055586131 8 0.2134146341463415 1.2841680425609978 9 0.13414634146341464 0.9080902586681342 >10 0.6341463414634146 7.61557512383049 >50 0.018292682926829267 0.8347092276646487 >100 0.006097560975609756 0.6902403228765364 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GGGTTTGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTT 190 0.4356998715831957 No Hit GGGTTTGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 111 0.2545404512933407 No Hit GGGTTTGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 77 0.17657310585213723 No Hit GGGTTTGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACAAAAAAAAAAAAAAA 63 0.14446890478811228 No Hit GGGTTTGGGGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 62 0.14217574756925333 No Hit GGGTTTGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTCTTTTTTTTTTTTTTT 58 0.13300311869381765 No Hit GGGTTTGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 53 0.12153733259952303 No Hit GGGTTTGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAACCAAAAAAAAAAAAAAA 51 0.11695101816180517 No Hit GGGTTTGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACAAAAAAAAAAAAAAA 48 0.1100715465052284 No Hit GGGTTTGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 47 0.10777838928636947 No Hit GGGTTTGGGGCCGAATAGCCTCTCCACCCAAGCGGCCGGAGAACCTGCGTGCAATC 45 0.10319207484865162 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.002293157218858925 0.0 0.0 0.0 0.0 10 0.061915244909190974 0.0 0.0 0.0 0.0 11 0.08484681709778022 0.0 0.0 0.0 0.0 12 0.09631260319207485 0.0 0.0 0.0 0.0 13 0.1008989176297927 0.0 0.0 0.0 0.0 14 0.12153733259952303 0.0 0.0 0.0 0.0 15 0.12612364703724088 0.0 0.0 0.0 0.0 16 0.14217574756925336 0.0 0.0 0.0 0.0 17 0.1467620620069712 0.0 0.0 0.0 0.0 18 0.17198679141441936 0.0 0.0 0.0 0.0 19 0.18803889194643184 0.0 0.0 0.0 0.0 20 0.23160887910475142 0.0 0.0 0.0 0.0 21 0.26141992294991745 0.0 0.0 0.0 0.0 22 0.2912309667950835 0.0 0.0 0.0 0.0 23 0.366905155017428 0.0 0.0 0.0 0.0 24 0.41506145661346544 0.0 0.0 0.0 0.0 25 0.4609246009906439 0.0 0.0 0.0 0.0 26 0.5113740598055403 0.0 0.0 0.0 0.0 27 0.5457714180884241 0.0 0.0 0.0 0.0 28 0.5985140341221794 0.0 0.0 0.0 0.0 29 0.6168592918730508 0.0 0.0 0.0 0.0 30 0.6329113924050633 0.0 0.0 0.0 0.0 31 0.6581361218125115 0.0 0.0 0.0 0.0 32 0.6650155934690882 0.0 0.0 0.0 0.0 33 0.671895065125665 0.0 0.0 0.0 0.0 34 0.6764813795633828 0.0 0.0 0.0 0.0 35 0.6948266373142543 0.0 0.0 0.0 0.0 36 0.6994129517519722 0.0 0.0 0.0 0.0 37 0.7200513667217024 0.0 0.0 0.0 0.0 38 0.731517152815997 0.0 0.0 0.0 0.0 39 0.731517152815997 0.0 0.0 0.0 0.0 40 0.7452760961291506 0.0 0.0 0.0 0.0 41 0.7498624105668684 0.0 0.0 0.0 0.0 42 0.7796734544120345 0.0 0.0 0.0 0.0 43 0.7980187121629059 0.0 0.0 0.0 0.0 44 0.8347092276646487 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position AAACACC 15 0.008168009 50.319817 38 CCGTCTC 15 0.008168009 50.319817 35 TCTCCTG 15 0.008168009 50.319817 38 AAAAACT 15 0.008168009 50.319817 35 CCTGCTT 15 0.008205547 50.261578 41 CTCCTGC 15 0.008205547 50.261578 39 AGAACCT 15 0.008205547 50.261578 39 TCCTGCT 15 0.008205547 50.261578 40 AACACCA 15 0.008205547 50.261578 39 AACCAAC 20 4.0337906E-4 50.261574 41 CCAACAC 25 2.0052647E-5 50.20347 43 CCAAAAC 60 0.0 50.20347 43 TGATATG 15 0.008318932 50.08766 50 GCAATCC 15 0.008318932 50.08766 50 AATAGCC 15 0.008318932 50.08766 14 AATGGTA 35 5.1088136E-8 50.029957 10 AGCATGT 55 0.0 50.029957 11 GAGCGCA 15 0.008356985 50.029953 11 AACTGTA 15 0.008356985 50.029953 10 AAGAGTG 15 0.008356985 50.029953 10 >>END_MODULE