FastQCFastQC Report
Fri 17 Jun 2016
SRR1526184_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526184_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences221777
Sequences flagged as poor quality0
Sequence length56
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGATCGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAG5960.26873841741929955No Hit
GAGATCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC4830.21778633492201627No Hit
GAGATCGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTC4040.18216496751241112No Hit
GAGATCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCCCCCGACTGCTCTTCC3860.17404870658364033No Hit
GAGATCGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGC3470.1564634745713036No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCCACG150.00813792150.43585637
CGTCACG150.00813792150.43585637
CATAAGT150.00830846750.17197449
ACTATTA204.1109126E-450.1605747
TATCTCA204.1109126E-450.1605748
ACGATTA252.0428106E-550.16056448
AACGATT252.0428106E-550.16056447
TACTATT204.1247753E-450.12636646
CATGCTC150.00833839650.1263646
ACTAAGG150.00833839650.1263645
ATTAACA150.00834589250.1149742
ACATTAA150.00840603150.0240450
TCTCGAC150.00843622249.978715
CTCGCGA150.00843622249.978715
GCCGGAT150.00845134749.95605513
CGTGGAT150.00845891849.94474431
CGGTAGG150.00845891849.94474431
GTTATAA150.00845891849.94474431
CGTTATA150.00846649349.93343730
TAACATA150.00846649349.93343730
Produced by FastQC (version 0.11.5)