FastQCFastQC Report
Fri 17 Jun 2016
SRR1526183_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526183_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences178360
Sequences flagged as poor quality0
Sequence length56
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGTACGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAG4230.2371607983852882No Hit
AAGTACGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGC3810.21361291769455035No Hit
AAGTACGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC3620.2029603050011213No Hit
AAGTACGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTC3250.18221574344023322No Hit
AAGTACGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCCCCCGACTGCTCTTCC2740.15362188831576587No Hit
AAGTACGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1880.10540479928235032No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAAGGG150.00817573750.37265841
ACTATTA301.0201948E-650.15927547
ACGATTA252.0414922E-550.15927548
AACGATT301.0201948E-650.15927547
CTAATCG301.0201948E-650.15927549
TAGGATC150.00832341550.14511534
TAATCGC301.0382246E-650.03211250
GTAATCC150.00842608949.9898733
GTTACCG150.00842608949.9898714
GTAAAGT150.00842608949.9898733
GCAGTAA150.00845425349.94769312
CGAATAG150.00845425349.94769312
ATTGGTT150.00845425349.94769310
CCGAATA150.00845425349.94769311
GAACCGT355.322545E-849.9196179
CAGTAAT150.00848248849.9055931
CTAACCG150.00849191549.89157526
GCCCTAC301.0585754E-649.89157526
ATACATC402.7193892E-949.87756323
TAGTACT150.0085013549.87756321
Produced by FastQC (version 0.11.5)