FastQCFastQC Report
Fri 17 Jun 2016
SRR1526182_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526182_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26091
Sequences flagged as poor quality0
Sequence length56
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCAACGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT420.16097504886742553No Hit
ATCAACGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCGCGCA290.1111494385036986No Hit
ATCAACGGGAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT290.1111494385036986No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCAAAAC150.00805501250.438643
GCACTGG150.00811743750.34046642
AGTACGT150.00830687350.04835546
ATAGCCT204.127636E-449.95173615
AATAGCC204.127636E-449.95173614
GAATAGC204.127636E-449.95173613
AGGGGAC150.00843497749.8554929
GACAGTG150.00843497749.855499
GAAGCTA150.00843497749.855499
AAGAGTG150.00843497749.8554910
GGCCGAA204.167252E-449.855499
AGTGGTC150.00843497749.8554912
GAAGCAG402.577508E-949.855499
TGTTCAC150.00843497749.8554912
TGCCCCC150.00843497749.8554912
GTCCCGC150.00843497749.8554911
GGCCCCC150.00843497749.855499
CACCCAA150.00843497749.8554925
CGAATAG204.167252E-449.8554912
GAACATG204.167252E-449.855499
Produced by FastQC (version 0.11.5)