##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1526182_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 26091 Sequences flagged as poor quality 0 Sequence length 56 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 37.373960369476066 39.0 38.0 39.0 33.0 40.0 2 36.59372963857269 39.0 38.0 39.0 30.0 40.0 3 36.41278601816718 39.0 37.0 39.0 30.0 40.0 4 37.107125062282016 39.0 38.0 39.0 33.0 40.0 5 36.74021693304204 39.0 37.0 39.0 32.0 40.0 6 35.76712276263846 39.0 35.0 39.0 28.0 40.0 7 36.23046261162853 38.0 36.0 39.0 30.0 40.0 8 36.31539611360239 39.0 36.0 39.0 30.0 40.0 9 36.200260626269596 39.0 36.0 39.0 30.0 40.0 10 35.63558315127822 38.0 35.0 39.0 28.0 40.0 11 35.533210685677055 38.0 35.0 39.0 29.0 40.0 12 33.3248629795715 38.0 32.0 39.0 19.0 40.0 13 33.04614618067533 37.0 31.0 39.0 18.0 40.0 14 33.29174811237591 37.0 32.0 39.0 19.0 40.0 15 33.272277796941474 38.0 32.0 39.0 19.0 40.0 16 32.666819976237015 36.0 30.0 39.0 18.0 40.0 17 32.77819937909624 36.0 30.0 39.0 20.0 40.0 18 32.209420873098004 36.0 29.0 38.0 17.0 40.0 19 33.09624008278717 36.0 30.0 38.0 22.0 40.0 20 32.97159940209268 36.0 30.0 38.0 22.0 40.0 21 33.77835268866659 37.0 33.0 39.0 23.0 40.0 22 33.55528726380744 37.0 32.0 39.0 23.0 40.0 23 33.96519872753056 38.0 33.0 39.0 24.0 40.0 24 33.64830784561726 37.0 32.0 39.0 23.0 40.0 25 33.75581618182515 37.0 33.0 39.0 23.0 40.0 26 34.59300141811352 38.0 34.0 39.0 26.0 40.0 27 34.39814495419876 38.0 33.0 39.0 26.0 40.0 28 34.371545743743056 38.0 33.0 39.0 25.0 40.0 29 34.250392855774024 38.0 33.0 39.0 25.0 40.0 30 34.226131616266144 38.0 33.0 39.0 25.0 40.0 31 34.4687823387375 38.0 34.0 39.0 27.0 40.0 32 34.2180062090376 38.0 33.0 39.0 25.0 40.0 33 34.28891188532444 38.0 34.0 39.0 26.0 40.0 34 34.21551492851941 38.0 34.0 39.0 26.0 40.0 35 34.05476984400751 38.0 33.0 39.0 25.0 40.0 36 33.21658809551186 36.0 32.0 39.0 24.0 39.0 37 32.977041891840095 36.0 32.0 39.0 23.0 39.0 38 32.756007818788085 35.0 32.0 39.0 22.0 39.0 39 32.434402667586525 35.0 31.0 38.0 19.0 39.0 40 29.14583572879537 35.0 23.0 38.0 6.0 39.0 41 25.5982905982906 31.0 13.0 37.0 4.0 39.0 42 29.706412172779885 34.0 28.0 38.0 4.0 39.0 43 28.164807788126172 33.0 24.0 38.0 4.0 39.0 44 28.096700011498218 33.0 23.0 38.0 4.0 39.0 45 29.209420873098004 33.0 27.0 38.0 4.0 39.0 46 28.787704572457937 34.0 26.0 38.0 4.0 39.0 47 28.337012763021733 34.0 24.0 38.0 4.0 39.0 48 28.41010310068606 34.0 25.0 38.0 4.0 39.0 49 25.882373232149018 32.0 11.0 38.0 4.0 39.0 50 28.04231344141658 33.0 24.0 38.0 4.0 39.0 51 28.119926411406233 35.0 23.0 39.0 4.0 39.0 52 27.819822927446246 34.0 22.0 38.0 4.0 39.0 53 27.40918324326396 34.0 18.0 38.0 4.0 39.0 54 26.507301368287916 33.0 10.0 38.0 4.0 39.0 55 26.878732129853205 33.0 8.0 38.0 4.0 39.0 56 25.995822314207963 33.0 4.0 38.0 4.0 39.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1 1 1.245565405954082 1 2 1.7693025686608976 1 3 1.9924201198267397 1 4 1.433101517032192 1 5 2.1038551757480093 1 6 3.200298356409 1 7 2.1862898202562846 1 8 2.291019043687527 1 9 2.2457253853855264 1 10 2.555876449948414 1 11 -2.7736334569432906 1 12 -13.427306393834339 1 13 -9.27241972944972 1 14 -6.339148197046121 1 15 -5.46455406940035 1 16 -22.11192005908415 1 17 -4.340451360330604 1 18 -12.187609798497839 1 19 -5.826409168483643 1 20 -5.235599852703338 1 21 -5.725045273127705 1 22 -2.6133864471946744 1 23 -3.3137871659453566 1 24 -4.09066389596558 1 25 -3.0126071424117242 1 26 1.931259240219795 1 27 1.7539060322909634 1 28 1.5533483097512217 1 29 2.686591944502595 1 30 1.3503961650357965 1 31 0.7936691384441303 1 32 1.5712888540045853 1 33 0.39670707930424953 1 34 0.37980573013637553 1 35 -0.9382707981607368 1 36 -0.8201275521454505 1 37 -0.5026357824509802 1 38 -0.4630399373358749 1 39 -1.089427829796552 1 40 3.2709023232688708 1 41 -9.149261561240934 1 42 -10.988465007073184 1 43 -16.671196273873292 1 44 -21.052514716901584 1 45 -12.079246796817772 1 46 -16.680694661096602 1 47 -22.11507868466221 1 48 -14.55790029651594 1 49 -19.135923468431482 1 50 -14.488365873096836 1 51 -11.793705557793517 1 52 -11.730904601155437 1 53 -12.394291168288122 1 54 -11.301995483638567 1 55 -11.969138099974378 1 56 -8.381100354585854 2 1 2.3492805762327222 2 2 2.9658969959054815 2 3 3.2710578907245704 2 4 2.4671572445863674 2 5 3.2075703460266496 2 6 3.978936127306831 2 7 3.475763504466812 2 8 3.2229075885791687 2 9 3.350988543280266 2 10 2.788074592363273 2 11 1.785190072468474 2 12 -1.0372135145773704 2 13 0.5898093727174611 2 14 3.7661149608486184 2 15 4.441018686017607 2 16 3.1543338108848893 2 17 3.3344712402885897 2 18 -1.1953497365783363 2 19 2.9862843299683703 2 20 3.52136609156911 2 21 3.287338627801084 2 22 3.5089045744771497 2 23 3.6598970445809584 2 24 2.83503269846166 2 25 2.9750089566594866 2 26 3.5535502618916226 2 27 3.356073214953497 2 28 3.8459179691939482 2 29 3.1788520064221046 2 30 4.2915726356240285 2 31 3.660542203459606 2 32 3.6889359128281143 2 33 4.2171405158367605 2 34 3.6274837487122227 2 35 3.990521771498706 2 36 4.049841488102231 2 37 4.901388985350881 2 38 4.728910527060414 2 39 3.6690861020919954 2 40 8.3885493820924 2 41 -5.937187257835362 2 42 -5.932737452893615 2 43 -12.239307728981652 2 44 -12.95653948470344 2 45 -5.418256084743469 2 46 -7.682242648712702 2 47 -17.723437817789147 2 48 -6.666259429642874 2 49 -11.236542663477923 2 50 -6.257715718298073 2 51 0.07007153198976468 2 52 0.21646381989719288 2 53 -0.03670602896923825 2 54 1.7568280457731973 2 55 1.1841126740194277 2 56 4.015184475135509 3 1 1.345724388466003 3 2 3.0896523301871284 3 3 3.423262727139935 3 4 2.621495793661758 3 5 3.285095239341011 3 6 3.806816639397873 3 7 3.226830360796825 3 8 3.4817980579959524 3 9 3.3673470070071474 3 10 2.8563533974841846 3 11 3.096000883279288 3 12 4.967765148296024 3 13 3.857945930327702 3 14 3.5257166678187417 3 15 3.996495215178342 3 16 5.660734948864974 3 17 6.328070102308505 3 18 5.772644573521237 3 19 4.306341228972634 3 20 5.935306347614624 3 21 4.527736920830961 3 22 4.609189069498491 3 23 3.348374996216805 3 24 4.595345642985137 3 25 4.991367420386368 3 26 4.072753675831876 3 27 3.6434131865039987 3 28 3.690868182565211 3 29 4.133332803008166 3 30 3.3591402031915933 3 31 3.2586929858209146 3 32 3.283530507422711 3 33 2.096230131768479 3 34 2.7868009606610187 3 35 2.5339072622526118 3 36 4.052686438315597 3 37 2.977491403558563 3 38 2.846975960915316 3 39 3.778616685306787 3 40 7.753414246957263 3 41 7.58841729408498 3 42 4.000406217092159 3 43 1.5330962539486457 3 44 3.453844583575364 3 45 5.004219021942166 3 46 2.8113562133072136 3 47 -2.4282742331518747 3 48 1.412687938778177 3 49 4.5635995840327475 3 50 2.0338916785724805 3 51 0.9662308492017111 3 52 0.94477107452024 3 53 0.887902790376426 3 54 2.108179397124548 3 55 0.7232307394532853 3 56 1.7349568791184389 4 1 2.0558595236011428 4 2 2.861949627484428 4 3 2.6644789433561513 4 4 2.4066309287968934 4 5 2.7141492933950673 4 6 3.1223571799384118 4 7 2.3112898202562846 4 8 2.3439602201581167 4 9 2.445725385385529 4 10 2.461758802889591 4 11 2.160190072468474 4 12 4.603575959106841 4 13 4.518756741138517 4 14 3.1108518029538814 4 15 3.33838710707024 4 16 5.042491705621732 4 17 5.39631334555175 4 18 4.863860789737455 4 19 4.56917906681047 4 20 4.930576617884899 4 21 3.7426017856958254 4 22 3.923378258687677 4 23 4.036212834054645 4 24 4.953453751093242 4 25 3.244745798764747 4 26 2.84302394610215 4 27 3.0494942675850822 4 28 2.682760074457107 4 29 3.4880625327378922 4 30 3.39157263562403 4 31 3.0039632560911897 4 32 4.013935912828117 4 33 2.715824726363074 4 34 3.257746906606961 4 35 3.6602586136039648 4 36 4.84457833020749 4 37 5.160599511666671 4 38 4.835489474428833 4 39 4.7414545231446255 4 40 9.088549382092403 4 41 11.09044432111201 4 42 7.8659467576327025 4 43 8.681744902597295 4 44 7.434249988980774 4 45 8.32516496788811 4 46 7.804599456550456 4 47 6.980509550631908 4 48 7.937687938778176 4 49 10.058194178627339 4 50 7.477810597491398 4 51 5.437176795147657 4 52 6.31909539884456 4 53 5.2027676552412885 4 54 6.731828045773199 4 55 6.211744252966799 4 56 6.308605527767089 5 1 2.3727514154930347 5 2 3.3869496274844266 5 3 3.801641105518314 5 4 2.864739036905 5 5 3.5013114555572287 5 6 3.9960058285870588 5 7 3.5781817121481794 5 8 3.5358521120500086 5 9 3.637617277277421 5 10 3.4239209650517495 5 11 3.6905954778738774 5 12 5.778575959106838 5 13 5.398486470868242 5 14 5.877068019170096 5 15 6.29379251247564 5 16 6.066140354270377 5 17 5.976718750957151 5 18 7.340212141088809 5 19 6.225260147891554 5 20 5.286657698965978 5 21 5.149358542452582 5 22 5.744324204633621 5 23 5.537564185405998 5 24 5.973724021363516 5 25 4.477853906872852 5 26 3.9376185406967465 5 27 4.5893591324499425 5 28 4.39357088526792 5 29 5.214413884089247 5 30 4.332113176164569 5 31 4.285720012847946 5 32 3.715962939855139 5 33 3.339473375011721 5 34 2.2462604201204783 5 35 2.885258613603966 5 36 4.485118870748032 5 37 4.707221133288289 5 38 4.603732717672074 5 39 4.778616685306787 5 40 9.34800884155186 5 41 12.183011888679573 5 42 7.351757568443514 5 43 8.803366524218916 5 44 8.075466205196989 5 45 6.977191994915135 5 46 7.514058916009915 5 47 6.544698739821094 5 48 7.709985236075475 5 49 8.509545529978695 5 50 6.547405192085996 5 51 7.695960578931441 5 52 7.809635939385103 5 53 6.914929817403451 5 54 7.027098316043471 5 55 6.452960469183015 5 56 6.464686608848169 6 1 2.186741876542314 6 2 3.2693025686608976 6 3 3.2571260021796817 6 4 2.609572105267482 6 5 3.1332669404538933 6 6 3.5238277681737102 6 7 2.6274662908445165 6 8 3.11454845545223 6 9 2.3045489147972944 6 10 2.320582332301356 6 11 2.726366543056706 6 12 5.190340664989193 6 13 5.256992035256161 6 14 4.719675332365647 6 15 5.03544593059965 6 16 4.770432882092319 6 17 5.836019227904689 6 18 6.371213730913926 6 19 4.438296713869299 6 20 3.2055766178848977 6 21 2.833778256284056 6 22 4.474848846922974 6 23 3.2744481281722884 6 24 2.7622772805050104 6 25 1.7520987399412178 6 26 1.784200416690382 6 27 2.2539060322909634 6 28 1.9651130156335768 6 29 2.7160037092084792 6 30 1.9974549885652024 6 31 1.6171985502088333 6 32 2.6007006187104693 6 33 1.1614129616571915 6 34 1.5268645536657885 6 35 1.3852586136039662 6 36 1.6798724478545495 6 37 1.9385406881372553 6 38 2.1546071214876577 6 39 2.4693956996152124 6 40 5.535608205621813 6 41 3.38015020346495 6 42 2.746829110573877 6 43 5.181744902597295 6 44 3.7710146948631227 6 45 3.7442826149469326 6 46 4.26048180949163 6 47 3.9731566094554367 6 48 4.2068055858369995 6 49 6.452311825686163 6 50 3.6292811857266933 6 51 3.5298238539711875 6 52 2.5926248106092693 6 53 3.782179419947173 6 54 4.845063339890846 6 55 4.824979547084446 6 56 5.0600761160023815 7 1 2.098506582424669 7 2 3.0340084510138396 7 3 3.4335965904149788 7 4 2.138983869973366 7 5 3.1626787051597702 7 6 3.9061807093501812 7 7 3.1568780555504006 7 8 2.526313161334585 7 9 3.3339606795031713 7 10 3.144111744066059 7 11 2.75577830776259 7 12 5.425634782636251 7 13 4.433462623491458 7 14 4.572616508836234 7 15 4.270740048246708 7 16 5.123374058562906 7 17 5.100725110257631 7 18 5.724154907384513 7 19 4.379473184457531 7 20 4.205576617884898 7 21 4.804366491578179 7 22 3.827790023393561 7 23 3.6568010693487594 7 24 2.2916890452108873 7 25 3.2226869752353338 7 26 2.195965122572737 7 27 1.2833177969968474 7 28 1.7592306626924028 7 29 2.3924742974437763 7 30 2.2915726356240285 7 31 2.9701397266794203 7 32 2.0418770892987013 7 33 2.2496482557748436 7 34 2.8503939654304915 7 35 3.855846848898082 7 36 2.8269312713839625 7 37 1.6738348057843133 7 38 2.7134306508994186 7 39 1.8517486407916834 7 40 5.182667029151226 7 41 -1.2669086200644628 7 42 4.099770287044468 7 43 4.769980196714943 7 44 4.947485283098416 7 45 3.920753203182226 7 46 3.5545994565504557 7 47 4.061391903573085 7 48 2.5891585270134705 7 49 4.481723590392047 7 50 1.4528105974913998 7 51 1.971000324559423 7 52 1.9749777517857403 7 53 1.6645323611236407 7 54 0.8156515751849618 7 55 0.9720383706138556 7 56 1.64831141012003 8 1 2.1835622263038417 8 2 2.6572198977547004 8 3 3.0178573217345317 8 4 1.5944687666347335 8 5 2.933743887989664 8 6 3.428438261019494 8 7 2.9295330634995267 8 8 2.806122382320275 8 9 2.772752412412551 8 10 3.126623667754451 8 11 3.393298180576579 8 12 5.535332715863596 8 13 4.884972957354734 8 14 4.82301396511604 8 15 4.077576296259423 8 16 5.6877619758920055 8 17 5.35509712933553 8 18 6.313185114061778 8 19 5.468503391134796 8 20 5.259630671938954 8 21 5.12233151542555 8 22 4.555135015444435 8 23 4.564591212433022 8 24 5.108859156498646 8 25 4.802178231197182 8 26 3.8835644866426904 8 27 3.7244942675850794 8 28 4.123300614997646 8 29 4.700900370575731 8 30 3.0888699329213267 8 31 2.0695037966317287 8 32 3.3646115885037915 8 33 1.5556895912279387 8 34 2.9219360957961484 8 35 2.5068802352255872 8 36 2.917551303180467 8 37 2.8964103224774824 8 38 3.5496786636180175 8 39 4.021859928550029 8 40 4.834495328038347 8 41 -0.276447570779883 8 42 4.000406217092159 8 43 5.370934091786481 8 44 5.724114853845634 8 45 3.436651454374598 8 46 5.243788645739642 8 47 5.166320361442722 8 48 5.764039290129531 8 49 8.131167151600316 8 50 4.9528105974914 8 51 4.80406868703955 8 52 4.94477107452024 8 53 6.212227114700749 8 54 7.270341559286713 8 55 5.8313388475613905 8 56 6.518740662902225 9 1 1.6594309521725705 9 2 2.6369496274844266 9 3 2.2823360862132986 9 4 1.1852023573683255 9 5 2.374863579109352 9 6 2.9187857513669826 9 7 1.650575534542 9 8 2.3225316487295444 9 9 0.8885825282426723 9 10 0.6903302314610187 9 11 1.3566186438970433 9 12 3.314290244821123 9 13 3.8473281697099466 9 14 3.589423231525309 9 15 1.995529964213091 9 16 3.881777419907447 9 17 4.107027631266032 9 18 5.95314650402317 9 19 2.6798933525247577 9 20 4.027005189313467 9 21 3.1068874999815392 9 22 2.548378258687677 9 23 2.9362128340546434 9 24 3.2748823225218118 9 25 2.691174370193316 9 26 2.9144525175307194 9 27 2.40306569615651 9 28 1.7613315030285364 9 29 3.7202053898807463 9 30 2.5058583499097438 9 31 1.207534684662619 9 32 2.2960787699709684 9 33 2.012253297791645 9 34 1.0898897637498166 9 35 2.420972899318251 9 36 1.8374354730646374 9 37 2.4427423688095224 9 38 1.1104894744288316 9 39 2.2593116660017714 9 40 4.459977953520973 9 41 0.7225871782548658 9 42 2.5766610433469843 9 43 3.860316331168722 9 44 3.109249988980771 9 45 -0.6569778892547475 9 46 2.197456599407598 9 47 1.99836669348905 9 48 0.09125936734960405 9 49 3.765337035770198 9 50 0.8456677403485422 9 51 -1.7663946334237686 9 52 -1.016618886869722 9 53 -2.5329466304729955 9 54 -1.6003148113696604 9 55 -2.9775414613189177 9 56 -1.0092516150900543 10 1 2.569094817718785 10 2 3.5634202157197237 10 3 3.7277142374737977 10 4 2.815454458208663 10 5 3.5450316463362412 10 6 4.435592474056058 10 7 3.3921721731974586 10 8 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114 55 -5.937168790511464 114 56 -2.7468292548416073 115 1 1.8882669310085447 115 2 2.6832459237807242 115 3 2.9357752396524504 115 4 2.1270012991672687 115 5 2.746556700802472 115 6 3.4214312540124894 115 7 2.464067598034063 115 8 2.5698861460840376 115 9 2.560540200200343 115 10 3.4839810251118095 115 11 -1.196291409013007 115 12 -5.99920181867094 115 13 -6.886798814417041 115 14 -9.37618523408316 115 15 -6.000501781818652 115 16 -4.582508294378268 115 17 -1.317575543337142 115 18 -2.640768839892175 115 19 -1.337302414671008 115 20 -1.6092381969299154 115 21 4.540749933843969 115 22 4.576156036465456 115 23 4.00102764886946 115 24 4.110861158500647 115 25 4.888264317283266 115 26 3.4911720942502953 115 27 1.8726424157332247 115 28 2.101278592975625 115 29 2.15935882903419 115 30 2.9026837467351427 115 31 3.0845188116467455 115 32 2.1704173943095952 115 33 3.35749139302974 115 34 1.7327469066069625 115 35 3.25562898397434 115 36 5.055689441318606 115 37 4.344858770925928 115 38 4.13826725220661 115 39 5.209047115737221 115 40 8.481141974684995 115 41 8.874703580371268 115 42 3.726131942817883 115 43 -5.410847689995297 115 44 -2.066675936945156 115 45 1.0612760789992208 115 46 -3.723178321227323 115 47 -3.1259719308495733 115 48 -3.068793542703304 115 49 -13.360324339891179 115 50 -3.158300513619711 115 51 -2.6063417233708606 115 52 -2.027200897451735 115 53 -2.9879730854994513 115 54 -4.761690472745322 115 55 -2.0965890803665346 115 56 1.7539758981374582 116 1 1.41419285693447 116 2 2.3869496274844266 116 3 2.7061456100228227 116 4 1.8232975954635648 116 5 2.4058159600617373 116 6 3.114023846605079 116 7 2.719623153589616 116 8 2.643960220158114 116 9 2.512392052052192 116 10 2.828425469556258 116 11 -1.8148099275315275 116 12 -13.154757374226495 116 13 -8.997909925528152 116 14 -5.505814863712789 116 15 -5.7116128929297645 116 16 -3.9491749610449354 116 17 -1.5620199877815857 116 18 -9.844472543595877 116 19 -2.5558209331895263 116 20 -4.227756715448436 116 21 2.525935119029157 116 22 2.065044925354343 116 23 2.4862128340546406 116 24 1.3701204177599067 116 25 1.169745798764744 116 26 1.6763572794354857 116 27 1.5911609342517465 116 28 1.7827600744571086 116 29 0.9630625327378937 116 30 1.7249059689573585 116 31 1.0622965894245198 116 32 1.2889359128281157 116 33 2.0241580596964113 116 34 1.2994135732736325 116 35 1.5519252802706305 116 36 3.7445783302074886 116 37 3.1522661783333383 116 38 2.3271561410955 116 39 3.2831211898112898 116 40 7.45521604875907 116 41 6.615444321112008 116 42 1.8409467576327003 116 43 -3.3849217640693716 116 44 -5.674083344352564 116 45 2.216831634554776 116 46 -0.9120672101162093 116 47 0.18884288396523985 116 48 -0.8873120612218237 116 49 -6.475139154705992 116 50 0.21947726415806557 116 51 0.3788434618143235 116 52 0.5024287321778971 116 53 -0.8138990114253772 116 54 -8.80983862089347 116 55 -0.8632557470332038 116 56 -1.5497278055662456 117 1 1.1475261902678042 117 2 2.053616294151091 117 3 2.306145610022817 117 4 1.4232975954635592 117 5 2.0058159600617316 117 6 2.728309560890793 117 7 2.0196231535896203 117 8 2.0725316487295444 117 9 2.126677766337906 117 10 2.261758802889588 117 11 -3.476714689436289 117 12 -14.411900231369351 117 13 -10.140767068385294 117 14 -10.434386292284216 117 15 -12.397327178644048 117 16 -15.72536543723541 117 17 -13.428686654448253 117 18 -15.677805876929211 117 19 -9.046297123665717 117 20 -9.22299481068653 117 21 -2.178826785732749 117 22 -1.106383646074228 117 23 -2.337596689754882 117 24 -2.6298795822400898 117 25 -2.392158963140016 117 26 1.8668334699116755 117 27 1.7244942675850794 117 28 1.8684743601713905 117 29 1.5773482470236075 117 30 2.0296678737192693 117 31 1.4813442084721373 117 32 1.2603644842566837 117 33 1.7384437739821266 117 34 0.5422707161307727 117 35 -0.4956937673484134 117 36 2.277911663540827 117 37 1.9427423688095224 117 38 0.5985847125240724 117 39 2.8069307136208153 117 40 5.555216048759064 117 41 3.282110987778676 117 42 -1.411434194748253 117 43 -8.29444557359318 117 44 -9.235988106257324 117 45 -0.38316836544522204 117 46 -2.0882576863066866 117 47 -2.2635380684157127 117 48 -1.4444549183646807 117 49 -6.317996297563134 117 50 -3.6662370215562206 117 51 -6.0878232048523415 117 52 -4.921380791631627 117 53 -5.294851392377758 117 54 -10.48126719232204 117 55 -6.715636699414155 117 56 -3.711632567471007 118 1 1.35585952360114 118 2 2.178616294151091 118 3 1.681145610022817 118 4 1.6732975954635592 118 5 2.3808159600617316 118 6 1.4723571799384132 118 7 2.5612898202562846 118 8 1.9772935534914495 118 9 0.6207253853855264 118 10 1.1784254695562524 118 11 -1.089809927531526 118 12 -5.138090707559829 118 13 -5.414576592194816 118 14 -6.880814863712789 118 15 -6.736612892929763 118 16 -9.957508294378268 118 17 -9.762019987781585 118 18 -8.886139210262545 118 19 -4.230820933189527 118 20 -5.21109004878177 118 21 0.35093511902915964 118 22 0.9233782586876771 118 23 1.519546167387979 118 24 -0.12987958224009333 118 25 0.20307913209808248 118 26 2.3013572794354857 118 27 3.557827600918415 118 28 1.7077600744571058 118 29 2.0297291994045565 118 30 2.4582393022906928 118 31 0.36229658942452403 118 32 -3.3527307538385536 118 33 1.6491580596964113 118 34 0.3994135732736268 118 35 0.9269252802706305 118 36 1.9445783302074915 118 37 1.8772661783333326 118 38 0.6938228077621673 118 39 2.199787856477961 118 40 4.930216048759064 118 41 2.407110987778676 118 42 -2.2923865757006325 118 43 -9.401588430736037 118 44 -12.182416677685895 118 45 -3.8415016987785577 118 46 -11.445400543449544 118 47 -12.852823782701426 118 48 -14.253978727888489 118 49 -11.175139154705994 118 50 -9.713856069175268 118 51 -8.962823204852342 118 52 -8.730904601155437 118 53 -8.99723234475871 118 54 -14.284838620893469 118 55 -9.388255747033202 118 56 -6.28306113889958 119 1 -0.11004956730795357 119 2 1.5233132638480598 119 3 1.7758425797197859 119 4 0.392994565160528 119 5 1.5209674752132472 119 6 2.1200844526656866 119 7 1.3226534566199177 119 8 1.780323856521747 119 9 1.6093617490218932 119 10 1.761758802889588 119 11 -5.851173563895163 119 12 -10.085060404529525 119 13 -14.922152349770574 119 14 -12.066420924318848 119 15 -10.702521983838853 119 16 -10.264326476196452 119 17 -14.428686654448253 119 18 -14.829321028444362 119 19 -7.901275478644074 119 20 -7.612605200296919 119 21 -0.6528527597587228 119 22 -0.6561671958577762 119 23 -1.1319689841271732 119 24 -0.9329098852703943 119 25 0.14247307149202015 119 26 -0.6115215084433032 119 27 0.8154033584941729 119 28 1.9009418926389259 119 29 -0.09148292180756101 119 30 -0.29933645528506503 119 31 -2.0164912893633584 119 32 -4.901973178080976 119 33 -1.036448000909651 119 34 -1.3127076388475842 119 35 -1.0238322954869403 119 36 1.3082146938438584 119 37 0.6765086025757618 119 38 1.3604894744288316 119 39 0.5709999776900787 119 40 4.615822109365126 119 41 1.7518079574756449 119 42 -2.777235060549117 119 43 -9.636436915584522 119 44 -10.004386374655592 119 45 -7.322562304839163 119 46 -14.672673270722271 119 47 -9.099035903913546 119 48 -10.996402970312733 119 49 -9.569078548645386 119 50 -10.95628031159951 119 51 -9.678732295761431 119 52 -8.139995510246347 119 53 -11.201777799304166 119 54 -12.561353772408621 119 55 -9.626892110669566 119 56 -8.570939926778365 120 1 0.7440174183379824 120 2 1.755370680116009 120 3 2.007899995987735 120 4 1.1250519814284772 120 5 1.7075703460266496 120 6 2.7157782325699955 120 7 2.054710872887867 120 8 1.9071181148949563 120 9 2.1141464380171087 120 10 1.788074592363273 120 11 -1.4516520327946836 120 12 -8.905634567208953 120 13 -6.015453785177275 120 14 -12.549674512835594 120 15 -9.90108657714029 120 16 -16.950929347009847 120 17 -8.849739286027202 120 18 -14.011139210262545 120 19 -4.382136722663212 120 20 -12.057581276851945 120 21 -5.081082424830491 120 22 -1.4647796360491654 120 23 -0.28747137647167165 120 24 -8.770230459433073 120 25 -1.6828857801826231 120 26 2.7114449987337323 120 27 2.935020583374552 120 28 2.3196021797202633 120 29 2.7051677958957896 120 30 2.2126252672029736 120 31 0.3710685192490857 120 32 -8.468958824013992 120 33 1.3224036737314933 120 34 1.1011679592385448 120 35 1.464205982025021 120 36 2.576157277575909 120 37 1.769810037982456 120 38 3.097331579691989 120 39 2.7217176810393653 120 40 6.78328622419766 120 41 2.1943916895330595 120 42 -4.4590532423673 120 43 -3.660360360560599 120 44 -4.5618026425981775 120 45 -5.312992926848732 120 46 -12.261190017133755 120 47 -13.276069396736514 120 48 -8.37678574543235 120 49 -17.104963716109502 120 50 -9.152452560403336 120 51 -7.719402152220763 120 52 -7.520378285365965 120 53 -7.984074450021868 120 54 -10.769487743700488 120 55 -6.973782062822675 120 56 -3.3532365774960695 >>END_MODULE >>Per sequence quality scores pass #Quality Count 4 311.0 5 99.0 6 47.0 7 26.0 8 55.0 9 72.0 10 38.0 11 44.0 12 56.0 13 64.0 14 75.0 15 83.0 16 98.0 17 116.0 18 167.0 19 192.0 20 222.0 21 279.0 22 360.0 23 432.0 24 471.0 25 486.0 26 584.0 27 673.0 28 807.0 29 937.0 30 1041.0 31 1295.0 32 1353.0 33 1582.0 34 1865.0 35 2175.0 36 2778.0 37 3154.0 38 2699.0 39 1354.0 40 1.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 0.0 100.0 0.0 0.0 2 0.0 0.0 100.0 0.0 3 0.0 0.0 0.0 100.0 4 0.0 100.0 0.0 0.0 5 0.0 100.0 0.0 0.0 6 0.0 0.0 0.0 100.0 7 98.96899313939672 0.9466865969108121 0.045992871104978725 0.03832739258748227 8 99.32927062971906 0.5250852784485072 0.0421601318462305 0.10348395998620215 9 97.52405043884865 1.805220190870415 0.4062703614273121 0.26445900885362766 10 41.21727798857844 41.85351270553064 7.615652907132728 9.313556398758193 11 30.819056379594496 31.61626614541413 20.244528764708136 17.320148710283238 12 34.3873366294891 16.883216434785943 21.723966118584954 27.00548081714001 13 25.072841588713384 17.539487808618308 26.702192915197053 30.685477687471245 14 23.027097466559347 20.677628300946687 28.224291901421942 28.07098233107202 15 24.961579837098512 20.32426617488858 27.535730751498388 27.178423236514522 16 27.364385662258 21.83247076864098 26.022618363043893 24.78052520605712 17 28.051818634778275 23.425702349469166 22.01525430224982 26.50722471350274 18 24.868718617041665 25.688987695963817 22.312085553298324 27.130208133696193 19 24.129334151580238 27.128720466400736 23.216477447069654 25.52546793494937 20 24.592365240744293 25.83924803376175 24.097448685977366 25.470938039516593 21 27.618719098539728 22.73197654363574 24.45287647081369 25.19642788701085 22 27.20095051933617 23.87030010348396 22.981104595454372 25.947644781725497 23 26.599210455712697 25.158100494423362 25.614196466214405 22.628492583649535 24 25.752175079529337 25.828829864704307 24.67517534782109 23.743819707945267 25 24.73266643670231 26.189107355026636 26.652868805335174 22.425357402935877 26 24.534304331161362 25.189727865082407 28.777309313913378 21.49865848984285 27 23.977752205600307 20.747986191024165 32.213271960107406 23.060989643268123 28 22.198350278150777 23.115288701323614 33.005946671782084 21.680414348743525 29 22.034353193773484 22.034353193773484 34.73276589218618 21.198527720266853 30 20.818798635335607 22.118296469505886 36.96860505232492 20.094299842833596 31 20.033734570267576 22.86667177796519 38.223568197500576 18.876025454266657 32 21.52966898085996 20.904453223888613 39.242836868551265 18.323040926700166 33 20.543424031929998 20.497371147868133 41.58191656752504 17.377288252676824 34 18.960162570453587 21.84732180514551 44.4653195813044 14.727196043096507 35 18.65726007438365 22.341934741766035 44.54967217514666 14.451133008703653 36 18.74472816501802 21.35572425427498 43.60478490913274 16.294762671574265 37 19.23062156870273 22.106192651744923 43.04142511613622 15.621760663416131 38 17.413852714071684 22.446314010218586 44.81195497675848 15.32787829895125 39 18.490073345877654 22.38777312699205 43.67343804001382 15.448715487116472 40 14.989962940086473 17.02825818406424 38.835701050030885 29.146077825818406 41 12.700007729767334 15.985158846718713 36.6661513488444 34.64868207466955 42 17.27168073676132 22.409823484267076 44.12125863392172 16.197237145049883 43 15.38254724227992 19.03134121984944 43.58196343524351 22.004148102627134 44 15.094194244327156 19.462957968948647 42.04646145548407 23.396386331240127 45 16.638513513513516 22.792536855036854 43.807585995085994 16.761363636363637 46 16.99105531882222 22.661138623363662 43.84045452800491 16.507351529809206 47 17.597208374875372 21.316818774445892 43.527110974768 17.55886187591073 48 17.4066541310104 23.477493380406 44.14597643808281 14.969876050500785 49 13.6003080477474 19.684251058914132 39.42241047362341 27.293030419715052 50 16.753002570891372 22.643029814665592 44.51479221825716 16.08917539618587 51 16.491834774255523 24.72622478386167 42.455331412103746 16.326609029779057 52 17.07532327999693 25.2484555466022 41.96308660450482 15.71313456889605 53 16.10117448376449 24.867582712827204 42.92239195517003 16.108850848238273 54 15.45465022473205 23.929161384503093 39.14563405170758 21.470554339057276 55 17.341373482869873 24.85788907666308 42.026424950069135 15.774312490397909 56 17.380787836903938 25.385855793595947 41.02357367734009 16.209782692160026 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.5 5 1.0 6 2.0 7 3.0 8 11.0 9 19.0 10 50.0 11 81.0 12 106.0 13 157.5 14 184.0 15 212.5 16 241.0 17 307.5 18 374.0 19 416.5 20 459.0 21 500.5 22 681.0 23 820.0 24 966.0 25 1112.0 26 1199.5 27 1287.0 28 1388.5 29 1471.0 30 1452.0 31 1291.0 32 1130.0 33 947.0 34 764.0 35 682.0 36 600.0 37 538.5 38 451.5 39 426.0 40 422.5 41 419.0 42 451.0 43 483.0 44 493.0 45 503.0 46 553.5 47 657.0 48 710.0 49 702.5 50 695.0 51 786.0 52 877.0 53 930.0 54 992.5 55 1002.0 56 1070.0 57 1138.0 58 1219.5 59 1301.0 60 1296.0 61 1291.0 62 1219.5 63 1045.0 64 942.0 65 829.5 66 717.0 67 622.5 68 528.0 69 451.5 70 375.0 71 336.5 72 265.0 73 232.0 74 181.0 75 130.0 76 98.5 77 67.0 78 49.0 79 20.5 80 10.0 81 9.0 82 8.0 83 5.5 84 3.0 85 2.5 86 2.0 87 1.0 88 0.0 89 0.0 90 0.5 91 1.0 92 0.5 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.026829174811237592 14 0.0 15 0.2414625733011383 16 0.022996435552489362 17 0.0 18 0.007665478517496454 19 0.07282204591621631 20 0.0996512207274539 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.003832739258748227 27 0.08048752443371276 28 0.0996512207274539 29 0.03449465332873405 30 0.015330957034992909 31 0.019163696293741136 32 0.07665478517496455 33 0.13031313479743972 34 0.03832739258748227 35 0.03832739258748227 36 0.03449465332873405 37 0.168640527384922 38 0.22996435552489364 39 0.19163696293741136 40 0.7167222413859184 41 0.8317044191483652 42 0.11881491702119505 43 0.2108006592311525 44 0.5135870606722625 45 0.16480778812617378 46 0.16097504886742553 47 0.04982561036372695 48 0.12264765627994327 49 0.46376145030853555 50 0.11498217776244682 51 0.252960791077383 52 0.11498217776244682 53 0.1418113525736844 54 0.22996435552489364 55 0.2108006592311525 56 0.17247326664367024 >>END_MODULE >>Sequence Length Distribution pass #Length Count 56 26091.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 78.64014411099612 #Duplication Level Percentage of deduplicated Percentage of total 1 87.33794716834 68.68268751676824 2 7.486109757286286 11.774175002874554 3 2.5002436884686614 5.898585719213522 4 1.072229262111317 3.37281054769844 5 0.5068720148162589 1.9930244145490783 6 0.2729310849010625 1.2878003909394042 7 0.18520323618286383 1.0195086428270284 8 0.18032946680963058 1.1344908205894753 9 0.10722292621113169 0.758882373232149 >10 0.35091139487279466 4.078034571308113 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source ATCAACGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 42 0.16097504886742553 No Hit ATCAACGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCGCGCA 29 0.1111494385036986 No Hit ATCAACGGGAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 29 0.1111494385036986 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.003832739258748227 0.0 0.0 0.0 0.0 10 0.015330957034992909 0.0 0.0 0.0 0.0 11 0.015330957034992909 0.0 0.0 0.0 0.0 12 0.019163696293741136 0.0 0.0 0.0 0.0 13 0.022996435552489366 0.0 0.0 0.0 0.0 14 0.026829174811237592 0.0 0.0 0.0 0.0 15 0.026829174811237592 0.0 0.0 0.0 0.0 16 0.03449465332873405 0.0 0.0 0.0 0.0 17 0.03449465332873405 0.0 0.0 0.0 0.0 18 0.03449465332873405 0.0 0.0 0.0 0.0 19 0.03449465332873405 0.0 0.0 0.0 0.0 20 0.03449465332873405 0.0 0.0 0.0 0.0 21 0.03832739258748227 0.0 0.0 0.0 0.0 22 0.04599287110497873 0.0 0.0 0.0 0.0 23 0.04599287110497873 0.0 0.0 0.0 0.0 24 0.05749108888122341 0.0 0.0 0.0 0.0 25 0.061323828139971635 0.0 0.0 0.0 0.0 26 0.07665478517496455 0.0 0.0 0.0 0.0 27 0.08048752443371278 0.0 0.0 0.0 0.0 28 0.08815300295120923 0.0 0.0 0.0 0.0 29 0.09581848146870568 0.0 0.0 0.0 0.0 30 0.10348395998620213 0.0 0.0 0.0 0.0 31 0.1111494385036986 0.0 0.0 0.0 0.0 32 0.11881491702119505 0.0 0.0 0.0 0.0 33 0.11881491702119505 0.0 0.0 0.0 0.0 34 0.12264765627994327 0.0 0.0 0.0 0.0 35 0.12264765627994327 0.0 0.0 0.0 0.0 36 0.12264765627994327 0.0 0.0 0.0 0.0 37 0.12264765627994327 0.0 0.0 0.0 0.0 38 0.1264803955386915 0.0 0.0 0.0 0.0 39 0.13031313479743972 0.0 0.0 0.0 0.0 40 0.13414587405618797 0.0 0.0 0.0 0.0 41 0.1379786133149362 0.0 0.0 0.0 0.0 42 0.1379786133149362 0.0 0.0 0.0 0.0 43 0.14564409183243263 0.0 0.0 0.0 0.0 44 0.14947683109118087 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CCAAAAC 15 0.008055012 50.4386 43 GCACTGG 15 0.008117437 50.340466 42 AGTACGT 15 0.008306873 50.048355 46 ATAGCCT 20 4.127636E-4 49.951736 15 AATAGCC 20 4.127636E-4 49.951736 14 GAATAGC 20 4.127636E-4 49.951736 13 AGGGGAC 15 0.008434977 49.85549 29 GACAGTG 15 0.008434977 49.85549 9 GAAGCTA 15 0.008434977 49.85549 9 AAGAGTG 15 0.008434977 49.85549 10 GGCCGAA 20 4.167252E-4 49.85549 9 AGTGGTC 15 0.008434977 49.85549 12 GAAGCAG 40 2.577508E-9 49.85549 9 TGTTCAC 15 0.008434977 49.85549 12 TGCCCCC 15 0.008434977 49.85549 12 GTCCCGC 15 0.008434977 49.85549 11 GGCCCCC 15 0.008434977 49.85549 9 CACCCAA 15 0.008434977 49.85549 25 CGAATAG 20 4.167252E-4 49.85549 12 GAACATG 20 4.167252E-4 49.85549 9 >>END_MODULE