FastQCFastQC Report
Fri 17 Jun 2016
SRR1526181_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526181_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19678
Sequences flagged as poor quality0
Sequence length56
%GC55

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCTCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC870.44211810143307245No Hit
GCCCTCGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTC760.386218111596707No Hit
GCCCTCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCCCCCGACTGCTCTTCC650.3303181217603415No Hit
GCCCTCGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT430.21851814208761053No Hit
GCCCTCGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGC430.21851814208761053No Hit
GCCCTCGGGGGGCTGGAGAGATGGCTCAGCCGTTAAAGGCTAGGCTCACAACCAAA410.20835450757190774No Hit
GCCCTCGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGACCACCCGACTGCTCTTC270.137209065961988No Hit
GCCCTCGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCGCGCA260.1321272487041366No Hit
GCCCTCGGGGGGGCTGGAGAGATGGCTCAGTGGTTAAGAGCACTGACTGCTCTTCC250.1270454314462852No Hit
GCCCTCGGGGACTTAAGCCAGCACTGCTGTGCGGGGCTCTGGCTGCGCCTGGTCTG230.11688179693058237No Hit
GCCCTCGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGCCATG210.10671816241487955No Hit
GCCCTCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCTGACTGCTCTTCC200.10163634515702816No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGAGAA150.00814760950.24678837
CGCCACC150.00814760950.24678827
AGAACCT150.00814760950.24678840
CCACTGA150.00814760950.24678840
CCCAAGC203.9844945E-450.24678827
CCCCGAC203.9844945E-450.24678841
CCCTGAC150.00814760950.24678840
ACCCAAG203.9844945E-450.24678826
CCCCCGA203.9844945E-450.24678840
GCCGTTA150.00814760950.24678829
AGCCGTT150.00814760950.24678828
AGACCAC203.9844945E-450.24678837
CAAGCGG203.9844945E-450.24678829
GGTTGGA150.00823103350.1179532
GTGCAAT150.00823103350.1179549
CGTTAAA150.00823103350.1179531
TGCAATC204.0355543E-450.1179550
TGCGTGC150.00823103350.1179546
TAAGACC204.0355543E-450.1179535
AACCTGC204.0355543E-450.1179542