##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1526181_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 19678 Sequences flagged as poor quality 0 Sequence length 56 %GC 55 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 38.123386523020635 39.0 38.0 39.0 35.0 40.0 2 37.51433072466714 39.0 38.0 39.0 34.0 40.0 3 37.90273401768472 39.0 38.0 39.0 35.0 40.0 4 37.87310702307145 39.0 38.0 39.0 35.0 40.0 5 37.36233357048481 39.0 38.0 39.0 33.0 40.0 6 37.633600975708916 39.0 38.0 39.0 35.0 40.0 7 37.61083443439374 39.0 38.0 39.0 35.0 40.0 8 37.696564691533695 39.0 38.0 39.0 35.0 40.0 9 37.808008943998374 39.0 38.0 39.0 35.0 40.0 10 37.40735847138937 39.0 38.0 39.0 35.0 40.0 11 36.49700172781787 38.0 36.0 39.0 31.0 40.0 12 34.55635735338957 38.0 34.0 39.0 23.0 40.0 13 34.147474336822846 38.0 33.0 39.0 23.0 40.0 14 34.234678320967575 38.0 33.0 39.0 23.0 40.0 15 34.260443134464886 38.0 33.0 39.0 23.0 40.0 16 33.593200528508994 38.0 32.0 39.0 21.0 40.0 17 33.40466510824271 37.0 31.0 39.0 21.0 40.0 18 32.92875292204492 36.0 31.0 39.0 19.0 40.0 19 33.70845614391706 37.0 31.0 39.0 23.0 40.0 20 33.86487447911373 37.0 32.0 39.0 24.0 40.0 21 34.21907714198598 38.0 33.0 39.0 24.0 40.0 22 33.968746823864215 38.0 33.0 39.0 24.0 40.0 23 34.5437544465901 38.0 33.0 39.0 26.0 40.0 24 34.15118406342108 38.0 33.0 39.0 24.0 40.0 25 34.413558288443944 38.0 33.0 39.0 25.0 40.0 26 34.846935664193516 38.0 35.0 39.0 27.0 40.0 27 34.695548328082126 38.0 34.0 39.0 26.0 40.0 28 34.67105396889928 38.0 34.0 39.0 26.0 40.0 29 34.47489582274621 38.0 33.0 39.0 26.0 40.0 30 34.42712674052241 38.0 33.0 39.0 26.0 40.0 31 34.50116881796931 38.0 34.0 39.0 26.0 40.0 32 34.200528508994815 38.0 33.0 39.0 25.0 40.0 33 34.27467222278687 38.0 33.0 39.0 25.0 39.0 34 34.060422807195856 38.0 33.0 39.0 25.0 39.0 35 33.90014229088322 37.0 33.0 39.0 25.0 39.0 36 33.65733306230308 37.0 33.0 38.0 25.0 39.0 37 33.35196666327879 36.0 32.0 38.0 23.0 39.0 38 33.07292407765017 36.0 32.0 38.0 23.0 39.0 39 32.596351255208866 36.0 32.0 38.0 19.0 39.0 40 29.667090151438153 35.0 25.0 38.0 9.0 39.0 41 26.97205000508182 32.0 17.0 38.0 6.0 39.0 42 30.627299522309176 35.0 29.0 38.0 10.0 39.0 43 29.3843378392113 34.0 26.0 38.0 5.0 39.0 44 29.197936782193313 34.0 26.0 38.0 4.0 39.0 45 30.179947149100517 35.0 28.0 38.0 4.0 39.0 46 29.776501676999697 35.0 27.0 38.0 4.0 39.0 47 29.320357759934954 35.0 26.0 38.0 4.0 39.0 48 29.356896026018905 35.0 26.0 38.0 4.0 39.0 49 26.915794288037404 33.0 16.0 38.0 4.0 39.0 50 28.903343835755667 34.0 25.0 38.0 4.0 39.0 51 29.195802418945014 35.0 26.0 38.0 4.0 39.0 52 28.97703018599451 35.0 26.0 38.0 4.0 39.0 53 28.571450350645392 35.0 24.0 38.0 4.0 39.0 54 27.586644984246366 34.0 18.0 38.0 4.0 39.0 55 27.832300030490902 34.0 21.0 38.0 4.0 39.0 56 26.605752617135888 33.0 17.0 38.0 4.0 39.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1 1 0.0839950984655502 1 2 0.8254040991532392 1 3 0.36514081925814423 1 4 0.4556906013130728 1 5 0.5537833895511213 1 6 0.22548072925677332 1 7 0.62357825123987 1 8 0.6829550783196439 1 9 0.5018666654736421 1 10 0.7067497978952701 1 11 -1.64959320063398 1 12 -15.166769422039955 1 13 -5.18219589392768 1 14 -4.984320633097916 1 15 -4.242572658488363 1 16 -23.51399358009471 1 17 -2.717426827648989 1 18 -13.154053938986017 1 19 -4.16473190435336 1 20 -4.047587518691714 1 21 -6.007621415555214 1 22 -3.1830121397244717 1 23 -2.236317744657512 1 24 -3.679213217431183 1 25 -2.9806172346221977 1 26 2.794334632091683 1 27 2.385041088558104 1 28 3.164832874641185 1 29 2.373523024884321 1 30 3.1048766092552853 1 31 3.1977944802366736 1 32 3.8512449924881764 1 33 2.766020991182806 1 34 3.086588787300265 1 35 3.333837133269462 1 36 4.079471517557103 1 37 3.584480305510624 1 38 3.4755474263567336 1 39 4.59538192727949 1 40 7.34946359608276 1 41 -5.083957622932466 1 42 -1.9202887220517617 1 43 -13.588191913168016 1 44 -21.172619626665906 1 45 -3.457357601998371 1 46 -11.937838426222644 1 47 -21.586982166070776 1 48 -9.530535285310663 1 49 -18.405931577485788 1 50 -8.682244353060696 1 51 -2.5947176301364365 1 52 -1.9985598369803625 1 53 -2.7984979495562747 1 54 -1.7706799012507304 1 55 -2.30137493288046 1 56 4.638590910553511 2 1 0.3720385767264176 2 2 0.7004040991532392 2 3 0.5698509641856759 2 4 0.7437340795739402 2 5 1.0700877373772073 2 6 0.5316401495466323 2 7 1.3753898454427684 2 8 1.3097666725225423 2 9 1.0833884046040794 2 10 0.7121845805039655 2 11 1.712725639945731 2 12 -2.936696958271842 2 13 1.2670794683911595 2 14 3.417853279945561 2 15 1.9711954574536676 2 16 2.9787600430936934 2 17 4.009022447713328 2 18 -1.2681843737686265 2 19 3.5272970811538897 2 20 2.4252385682648097 2 21 3.0286104685027553 2 22 3.5706110486813216 2 23 2.9158561683859645 2 24 0.9294824347427308 2 25 3.2512668233488142 2 26 2.5552041973090738 2 27 1.5825048566740492 2 28 2.5470792514527787 2 29 1.6180882422756255 2 30 3.3711809570813713 2 31 2.5365625961787046 2 32 3.554143543212817 2 33 2.708049976690056 2 34 2.736951106140843 2 35 0.9841994521100403 2 36 1.999761372629571 2 37 2.8000600156555464 2 38 2.033518440849484 2 39 1.9685703330765918 2 40 5.331347654053776 2 41 -10.127435883802033 2 42 -6.528984374225676 2 43 -14.238554232008594 2 44 -13.585663104926777 2 45 -5.361343109244746 2 46 -8.926968861005253 2 47 -17.527199557375123 2 48 -8.354810647629506 2 49 -13.300859113717673 2 50 -5.979345802336059 2 51 -1.7360219779625226 2 52 -0.9442120108934056 2 53 -1.0177008481069976 2 54 0.5028708233869494 2 55 -1.6944908749094445 2 56 2.6422140989593075 3 1 0.6508847305725709 3 2 0.8638656376147793 3 3 1.2012612205959314 3 4 0.8302725411124001 3 5 0.7527800450695139 3 6 1.4194606623671433 3 7 0.7824411274940459 3 8 1.1495102622661264 3 9 0.8814653276809992 3 10 1.2217999651193523 3 11 2.73836666558676 3 12 4.739585093010213 3 13 1.1388743401860353 3 14 2.552468664560948 3 15 4.6955544318126385 3 16 4.850554914888569 3 17 5.678894242585123 3 18 4.99143101084676 3 19 4.597809901666707 3 20 5.069469337495576 3 21 4.554251494143784 3 22 4.057790535860811 3 23 3.4158561683859645 3 24 4.429482434742731 3 25 3.7384463105283032 3 26 1.7475118896167672 3 27 2.7395561387253267 3 28 3.261822841196363 3 29 2.935395934583319 3 30 3.1115655724659845 3 31 2.2128446474607557 3 32 3.105425594494868 3 33 3.4901012587413334 3 34 3.9260536702434123 3 35 0.6348404777510694 3 36 2.935658808527002 3 37 3.181470272065802 3 38 2.559159466490513 3 39 3.0519036664099275 3 40 5.722373295079418 3 41 7.795641039274887 3 42 4.701784856543558 3 43 1.2646508961965317 3 44 0.7989522796886064 3 45 5.770067147165506 3 46 4.899954215917827 3 47 -0.7291226342982 3 48 4.302240634421775 3 49 5.69273062987207 3 50 4.110397787407532 3 51 4.321670329729784 3 52 5.651941835260441 3 53 5.517555562149415 3 54 4.102229797745924 3 55 2.2253809199623475 3 56 1.6870858938310995 4 1 0.9970385767264176 4 2 0.6330964068455458 4 3 1.3166458359805517 4 4 0.253349464189327 4 5 0.291241583531054 4 6 0.4579222008286834 4 7 0.28244112749404593 4 8 0.2264333391892066 4 9 0.34300378921946617 4 10 0.1833384266578122 4 11 1.1614435886636798 4 12 2.816508169933286 4 13 3.7927204940321886 4 14 3.3986225107147945 4 15 2.503246739504945 4 16 3.9659395302731824 4 17 4.448125011815897 4 18 4.529892549308293 4 19 3.982425286282094 4 20 4.26177702980327 4 21 2.938866878759171 4 22 3.827021305091577 4 23 2.8389330914628843 4 24 3.6602516655119643 4 25 2.7384463105283032 4 26 2.9013580434629205 4 27 2.89340229257148 4 28 2.915668995042523 4 29 3.2046267038140854 4 30 2.842334803235218 4 31 2.1743831089992156 4 32 1.9515794406487146 4 33 1.9516397202798004 4 34 2.9645152087049453 4 35 2.2502250931356826 4 36 3.935658808527002 4 37 4.296854887450422 4 38 4.597621004952053 4 39 5.0519036664099275 4 40 7.9531425258486514 4 41 9.98794873158258 4 42 7.317169471928171 4 43 7.533881665427305 4 44 7.29895227968861 4 45 5.346990224088586 4 46 7.05380036976398 4 47 6.693954288778723 4 48 7.840702172883315 4 49 9.231192168333607 4 50 5.725782402792149 4 51 6.590901098960558 4 52 5.575018758337361 4 53 6.632940177534028 4 54 6.948383643899771 4 55 4.763842458423888 4 56 7.379393586138793 5 1 0.3047308844187242 5 2 1.6330964068455458 5 3 1.1627996821343984 5 4 0.9456571564970204 5 5 1.7143185066079738 5 6 1.6886914315979098 5 7 1.3978257428786662 5 8 1.4187410314969 5 9 1.1506960969117728 5 10 1.4910307343501188 5 11 2.5075974348175265 5 12 4.701123554548673 5 13 4.369643570955262 5 14 4.9370840491763275 5 15 4.580169816428025 5 16 5.350554914888569 5 17 4.909663473354357 5 18 6.222200241615987 5 19 4.6747329785897875 5 20 5.031007799034043 5 21 2.977328417220704 5 22 3.0577905358608106 5 23 3.685086937616731 5 24 3.1217901270504242 5 25 4.00767707975907 5 26 2.8244349665398403 5 27 3.1241715233407135 5 28 3.0695151488886765 5 29 3.5892420884294722 5 30 3.419257880158291 5 31 3.443613878229982 5 32 3.9900409791102476 5 33 3.1824089510490268 5 34 3.233745977935719 5 35 3.1733020162126095 5 36 4.820274193142389 5 37 4.796854887450422 5 38 5.82839023572128 5 39 4.590365204871468 5 40 8.837757910464031 5 41 11.641794885428734 5 42 6.971015625774324 5 43 9.072343203888838 5 44 9.414336895073223 5 45 8.462374839473199 5 46 7.05380036976398 5 47 7.8093389041633365 5 48 8.033009865191008 5 49 10.115807552948993 5 50 8.418090095099842 5 51 8.821670329729784 5 52 9.151941835260441 5 53 9.094478639072488 5 54 9.44838364389977 5 55 8.994611689193121 5 56 8.571701278446486 6 1 0.8666037941177223 6 2 2.042795403501067 6 3 1.4955756018668396 6 4 1.5861253839217682 6 5 1.1624790417250352 6 6 1.703741598821992 6 7 0.62357825123987 6 8 1.6829550783196439 6 9 1.4583884046040794 6 10 1.8806628413735282 6 11 1.9156241906703713 6 12 4.485404491003521 6 13 3.991717149550581 6 14 5.189592410380342 6 15 4.192209950207285 6 16 5.6164412025139825 6 17 6.021703607133617 6 18 5.454641713187897 6 19 5.530920269559687 6 20 5.169803785656114 6 21 4.122813367053482 6 22 4.208292208101611 6 23 4.6332474727337924 6 24 4.581656347786208 6 25 4.236774069725627 6 26 3.4899868060047226 6 27 2.6893889146450647 6 28 3.903963309423794 6 29 4.11265345966693 6 30 3.7135722614291993 6 31 3.936924915019283 6 32 4.546897166401223 6 33 4.1138470781393295 6 34 1.8257192220828742 6 35 4.899054524573813 6 36 3.644688908861454 6 37 3.6714368272497495 6 38 4.605982208965429 6 39 5.899729753366451 6 40 8.479898378691463 6 41 1.524738029241444 6 42 6.123189538817801 6 43 8.107460260745025 6 44 6.0447716776819185 6 45 6.238294571914672 6 46 6.279552878125184 6 47 8.45649609479879 6 48 7.425986453819771 6 49 10.333198857296821 6 50 8.143842603461046 6 51 7.883543239428782 6 52 9.001440163019641 6 53 9.636284659139381 6 54 10.88149401179275 6 55 9.04645115407606 6 56 8.334243084466554 7 1 0.1220385767264176 7 2 1.2420707658199035 7 3 0.7365176308523473 7 4 0.9104007462406045 7 5 0.403421070710543 7 6 1.2816401495466323 7 7 0.7503898454427684 7 8 0.9764333391892066 7 9 1.0000550712707437 7 10 0.2538512471706298 7 11 2.337725639945731 7 12 4.354969708394826 7 13 4.5170794683911595 7 14 4.667853279945561 7 15 5.471195457453668 7 16 4.312093376427029 7 17 5.675689114379999 7 18 4.773482292898038 7 19 1.9022970811538897 7 20 1.966905234931474 7 21 3.861943801836091 7 22 3.3206110486813216 7 23 4.082522835052629 7 24 3.596149101409395 7 25 2.95960015668215 7 26 3.1802041973090738 7 27 2.2491715233407135 7 28 3.213745918119443 7 29 3.03475490894229 7 30 2.4961809570813713 7 31 2.7865625961787046 7 32 3.6374768765461454 7 33 2.49971664335672 7 34 2.986951106140843 7 35 2.9008661187767117 7 36 2.999761372629571 7 37 4.008393348988882 7 38 3.6168517741828197 7 39 4.510236999743263 7 40 8.248014320720447 7 41 3.0808974495312995 7 42 6.721015625774324 7 43 7.969779101324736 7 44 7.247670228406559 7 45 6.846990224088586 7 46 7.198031138994747 7 47 8.222800442624877 7 48 5.103522685703826 7 49 8.57414088628233 7 50 5.978987530997273 7 51 6.180644688704142 7 52 5.805787989106594 7 53 6.27396581855967 7 54 6.336204156720282 7 55 5.263842458423888 7 56 6.10054743229264 8 1 0.7278078074956511 8 2 1.786942560691699 8 3 1.2781842975190116 8 4 1.3302725411124001 8 5 1.291241583531054 8 6 1.6117683546748367 8 7 1.320902665955586 8 8 1.0341256468815132 8 9 1.4199268661425393 8 10 1.7217999651193523 8 11 3.0460589732790666 8 12 4.547277400702519 8 13 3.8311820324937287 8 14 4.744776356868641 8 15 4.310939047197252 8 16 5.196708761042416 8 17 5.2942788579697435 8 18 5.337584857000607 8 19 5.443963747820554 8 20 3.37716164518789 8 21 2.977328417220704 8 22 2.5193289973992705 8 23 3.762010014539811 8 24 3.929482434742731 8 25 3.7384463105283032 8 26 2.9013580434629205 8 27 2.5087869079561003 8 28 3.1079766873502095 8 29 3.7815497807371656 8 30 3.419257880158291 8 31 3.2128446474607557 8 32 3.720810209879481 8 33 3.9131781818182603 8 34 2.772207516397259 8 35 3.5579174008279892 8 36 2.858735731603929 8 37 3.143008733604269 8 38 2.482236389567433 8 39 2.359595974102234 8 40 5.0300656027717245 8 41 -2.012051268417416 8 42 2.701784856543558 8 43 4.187727819273459 8 44 5.29895227968861 8 45 4.077759454857819 8 46 3.399954215917827 8 47 4.270877365701796 8 48 4.340702172883315 8 49 6.269653706795147 8 50 5.225782402792149 8 51 4.667824175883631 8 52 4.305787989106594 8 53 5.479094023687875 8 54 6.4099221054382305 8 55 3.2253809199623475 8 56 5.571701278446486 9 1 -1.263830988490973 9 2 -6.828573685027095E-4 9 3 -0.4609461372635977 9 4 -0.32691809433910635 9 5 -0.22882530610105078 9 6 -0.2527801403084453 9 7 -1.2894652270210045 9 8 -0.1866101390716608 9 9 -0.36769855191766254 9 10 -0.07585889775690191 9 11 1.0460589732790666 9 12 2.96366536056874 9 13 1.252586714767972 9 14 2.5808967582064284 9 15 3.2356882110768552 9 16 3.8773107677313803 9 17 3.760834041916226 9 18 3.80246780014442 9 19 3.7048333130379447 9 20 3.5176298726126376 9 21 1.7315090192273956 9 22 0.773509599405962 9 23 1.0245518205598785 9 24 1.5381780869166448 9 25 1.5411218958125872 9 26 0.5334650668742924 9 27 1.428519349427667 9 28 1.0778763529020523 9 29 1.2430882422756255 9 30 1.0613983483857226 9 31 0.6325770889323223 9 32 1.1555928185751299 9 33 0.9399340346610714 9 34 0.26050183077853006 9 35 1.55122843761729 9 36 1.6881671697310168 9 37 2.0627411750758355 9 38 1.7364169915741243 9 39 2.682338449018623 9 40 4.219028813474065 9 41 2.655172811850143 9 42 3.6884069301221523 9 43 5.237895043353721 9 44 4.175206460290614 9 45 2.6295989197407614 9 46 4.6273789650817 9 47 4.413017833929228 9 48 4.16511688860238 9 49 6.202764074688126 9 50 3.926451299113218 9 51 2.7531084568200868 9 52 3.784048858671813 9 53 4.244980311313292 9 54 4.446711403097094 9 55 3.611668545380411 9 56 4.638590910553511 10 1 0.6266682063560438 10 2 0.6031818769310178 10 3 0.3152213345560497 10 4 0.4798451906850545 10 5 1.0932358855253526 10 6 0.5733068162132966 10 7 1.0003898454427684 10 8 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3.5054125847861073 118 29 2.7430882422756255 118 30 3.7184031793035928 118 31 1.1060070406231475 118 32 -7.0569675678982975 118 33 0.9302721989122773 118 34 1.5841733283630646 118 35 1.886977229887819 118 36 0.11087248374067826 118 37 0.5361711267666607 118 38 -1.744259336928291 118 39 0.6074592219654846 118 40 4.4702365429426685 118 41 2.4281196717535245 118 42 -0.6956510408923435 118 43 -11.141332009786371 118 44 -9.696774216037888 118 45 -2.5418986648003035 118 46 -9.621413305449696 118 47 -11.541088446264013 118 48 -9.813143980962838 118 49 -12.550859113717673 118 50 -7.812679135669391 118 51 -7.54157753351808 118 52 -6.249767566448963 118 53 -7.1149230703292226 118 54 -10.927684732168604 118 55 -7.5694908749094445 118 56 -3.552230345485139 119 1 0.3970385767264162 119 2 0.4920707658199035 119 3 0.5448509641856774 119 4 0.7020674129072759 119 5 1.278421070710543 119 6 0.9733068162132952 119 7 1.1337231787761013 119 8 1.1931000058558752 119 9 0.9250550712707479 119 10 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119 49 -7.81752578038434 119 50 -3.746012469002725 119 51 -1.3193553112958547 119 52 -0.4942120108934063 119 53 -0.39270084810699757 119 54 -4.005462509946383 119 55 0.1305091250905548 119 56 3.925547432292639 120 1 -1.3875768078889692 120 2 -1.1745959008467608 120 3 -1.2602772409425285 120 4 -1.0158813050414466 120 5 -2.131835339545873 120 6 -0.8113085684020902 120 7 -1.0252511801982607 120 8 -0.8120281992723335 120 9 -0.9262269800113074 120 10 -2.1628154194960345 120 11 -5.80009487287478 120 12 -17.106568753143634 120 13 -16.861125659813965 120 14 -13.947531335439056 120 15 -13.265984029725821 120 16 -10.457137392803741 120 17 -16.282644218953337 120 18 -13.739338219922473 120 19 -7.325267021410216 120 20 -13.16129989327365 120 21 -4.368825428933139 120 22 -2.249901771831496 120 23 -1.5841438316140355 120 24 -10.378209872949576 120 25 -2.64616907408708 120 26 -1.6371034949986196 120 27 -1.7989053997362063 120 28 -0.8151002357267103 120 29 -1.3722963731089877 120 30 -1.8115113506109353 120 31 -3.210232275616171 120 32 -11.971497482428212 120 33 -2.548360279720203 120 34 -2.920100175910438 120 35 -3.2113133684027773 120 36 -1.9104950376268413 120 37 -1.241606651011118 120 38 -1.440840533509487 120 39 -3.294250179743919 120 40 1.530065602771728 120 41 1.4879487315825841 120 42 -4.528984374225676 120 43 -6.581502949957311 120 44 -11.0087400280037 120 45 -2.383779006680644 120 46 -11.446199630236023 120 47 -12.767584172759738 120 48 -8.390067057885915 120 49 -16.191884754743313 120 50 -7.120371443361698 120 51 -7.9860219779625226 120 52 -6.078827395508789 120 53 -7.059367514773665 120 54 -10.897770202254074 120 55 -9.505388310806882 120 56 -7.27445256770736 >>END_MODULE >>Per sequence quality scores pass #Quality Count 4 36.0 5 4.0 6 9.0 7 16.0 8 37.0 9 91.0 10 52.0 11 44.0 12 40.0 13 20.0 14 50.0 15 50.0 16 62.0 17 80.0 18 100.0 19 137.0 20 114.0 21 173.0 22 230.0 23 277.0 24 305.0 25 357.0 26 416.0 27 485.0 28 540.0 29 619.0 30 759.0 31 955.0 32 1055.0 33 1240.0 34 1412.0 35 1765.0 36 2195.0 37 2797.0 38 2171.0 39 985.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 100.0 0.0 0.0 0.0 2 0.0 0.0 0.0 100.0 3 0.0 0.0 0.0 100.0 4 0.0 0.0 0.0 100.0 5 0.0 0.0 100.0 0.0 6 0.0 0.0 0.0 100.0 7 98.98363654842971 0.45736355320662675 0.20835450757190774 0.3506453907917471 8 98.7549547718264 0.5285089948165465 0.5437544465901006 0.17278178676694786 9 97.27614594979165 1.5804451671917876 0.43703628417522106 0.7063725988413457 10 57.50076227258868 30.379103567435717 5.92031710539689 6.199817054578717 11 42.28580140258156 25.05844089846529 15.799369854660025 16.85638784429312 12 39.61784734220957 14.264661042788902 20.002032726903142 26.115458888098388 13 27.925180441191422 13.749110501169056 24.804310257192235 33.52139880044729 14 22.635564364486456 17.56873507140316 30.111297453880166 29.684403110230217 15 26.155178562331244 16.822049009119162 30.113607417596413 26.909165010953178 16 33.78928099257602 18.870131190887825 24.488965727651784 22.85162208888437 17 31.21252159772335 21.97885964020734 23.274723040959447 23.533895721109868 18 30.973676186604333 21.597723345868484 23.523732086594165 23.904868380933024 19 29.69878905057495 26.198229368067572 20.3673552457515 23.735626335605982 20 31.395289689200872 25.16404700137342 21.298133170558014 22.142530138867695 21 31.558085171257243 23.75749568045533 22.80719585323712 21.87722329505031 22 28.803740217501776 22.64457770098587 25.77497713182234 22.77670494969001 23 30.201239963410913 22.293932310194126 25.464986279093406 22.039841447301555 24 31.30399430836467 21.033641630246976 23.57963207643053 24.08273198495782 25 27.202967781278588 23.411932106921434 22.405732289866855 26.979367821933124 26 25.810714648775036 22.257802175459997 25.353258107146488 26.578225068618483 27 22.866737902610286 20.368391594158652 27.38004375922251 29.384826744008546 28 22.162299669295344 23.520732638005594 27.23988806919359 27.07707962350547 29 27.800091514566066 22.634602674258986 26.117240327418777 23.448065483756164 30 25.76250508336722 19.830215534770232 30.647620984139895 23.759658397722653 31 28.30342162794245 21.068686765976917 30.18455437490467 20.443337231175963 32 30.62086513994911 19.704834605597966 29.669211195928753 20.005089058524174 33 29.754632457747913 19.257788637751986 32.64100997760131 18.346568926898797 34 24.31992678090202 19.921696242436568 38.607820206437175 17.150556770224235 35 21.480502313284862 25.934211195281915 37.49555137526056 15.089735116172657 36 22.80353874313606 27.740492170022375 32.499491559894246 16.956477526947324 37 24.233628679091556 26.662592932070478 30.843263061411548 18.26051532742642 38 24.69934773746433 25.524867509172445 32.73542600896861 17.040358744394617 39 26.11108282848852 21.76856895586214 31.767041694242227 20.353306521407116 40 18.80499283814201 16.63085737671373 29.205033763044813 35.35911602209944 41 15.320391333299185 16.267991599651694 27.62895046867797 40.78266659837115 42 19.389468328669548 22.147036377512084 33.60468074281353 24.858814551004834 43 17.483443708609272 17.203260315843096 35.11462047885889 30.198675496688743 44 19.79677287581699 17.366217320261438 34.41074346405229 28.426266339869283 45 22.94297352342159 21.125254582484725 33.58452138492872 22.34725050916497 46 20.78219687324948 22.54926923664511 34.41462545195294 22.253908438152468 47 19.50388857825446 21.252478015554313 35.292024602246734 23.951608803944495 48 21.1185181415704 20.899699760826422 37.97262225840924 20.009159839193934 49 19.703703703703702 16.69987228607918 32.55172413793103 31.04469987228608 50 20.127194098193844 20.925973034851182 37.05418468583058 21.892648181124397 51 19.315054530628885 21.49627968606666 37.43757007440628 21.751095708898173 52 19.788327481809393 21.543784663918995 36.482979697756065 22.184908156515544 53 18.881368008550055 21.319151101837246 39.00452949259504 20.794951397017662 54 17.733965051709205 20.296500076417544 35.895868358041675 26.07366651383158 55 19.286187057685453 21.226006822463216 36.39325899903263 23.094547120818696 56 19.87479641693811 23.870114006514658 33.26547231270358 22.98961726384365 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 1.0 9 2.0 10 3.0 11 4.0 12 4.0 13 3.5 14 3.0 15 7.5 16 12.0 17 21.5 18 31.0 19 40.0 20 49.0 21 65.5 22 120.0 23 158.0 24 175.0 25 192.0 26 193.0 27 194.0 28 236.0 29 350.0 30 422.0 31 490.5 32 559.0 33 628.0 34 697.0 35 703.5 36 710.0 37 641.5 38 475.0 39 377.0 40 362.0 41 347.0 42 315.0 43 283.0 44 283.0 45 283.0 46 302.0 47 316.0 48 311.0 49 350.5 50 390.0 51 413.0 52 436.0 53 489.0 54 580.0 55 618.0 56 693.0 57 768.0 58 830.0 59 892.0 60 1006.0 61 1120.0 62 1249.5 63 1493.0 64 1607.0 65 1587.5 66 1568.0 67 1448.0 68 1328.0 69 1160.5 70 993.0 71 847.5 72 616.5 73 531.0 74 448.5 75 366.0 76 312.0 77 258.0 78 197.0 79 109.5 80 83.0 81 60.0 82 37.0 83 27.0 84 17.0 85 13.0 86 9.0 87 6.0 88 2.5 89 2.0 90 1.0 91 0.0 92 0.5 93 1.0 94 0.5 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.02032726903140563 14 0.0050818172578514075 15 0.24900904563471896 16 0.0609818070942169 17 0.0 18 0.0 19 0.1219636141884338 20 0.09655452789917675 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.02032726903140563 27 0.1270454314462852 28 0.11688179693058237 29 0.04573635532066267 30 0.03049090354710845 31 0.04573635532066267 32 0.14229088321983943 33 0.17278178676694786 34 0.05589998983636549 35 0.04573635532066267 36 0.05081817257851408 37 0.20327269031405631 38 0.274418131923976 39 0.17786360402479928 40 0.660636243520683 41 0.7876816749669682 42 0.11688179693058237 43 0.2439272283768676 44 0.47769082223803233 45 0.1931090557983535 46 0.20835450757190774 47 0.02540908628925704 48 0.137209065961988 49 0.5234271775586951 50 0.11688179693058237 51 0.28458176643967886 52 0.1270454314462852 53 0.14737270047769083 54 0.24900904563471896 55 0.1880272385405021 56 0.15245451773554222 >>END_MODULE >>Sequence Length Distribution pass #Length Count 56 19678.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 76.83199512145544 #Duplication Level Percentage of deduplicated Percentage of total 1 86.74515510285072 66.64803333672121 2 7.98994642502811 12.277670494969001 3 2.5927640716978635 5.976217095233256 4 1.0648852437330512 3.272690314056306 5 0.4431510020504002 1.7024087813802218 6 0.32409550896223294 1.494054273808314 7 0.1918116277531583 1.0316089033438356 8 0.15212646339043587 0.935054375444659 9 0.04629935842317614 0.3201544872446387 >10 0.4299226139294927 5.183453603008436 >50 0.0198425821813612 1.1586543347901208 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GCCCTCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC 87 0.44211810143307245 No Hit GCCCTCGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTC 76 0.386218111596707 No Hit GCCCTCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCCCCCGACTGCTCTTCC 65 0.3303181217603415 No Hit GCCCTCGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 43 0.21851814208761053 No Hit GCCCTCGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGC 43 0.21851814208761053 No Hit GCCCTCGGGGGGCTGGAGAGATGGCTCAGCCGTTAAAGGCTAGGCTCACAACCAAA 41 0.20835450757190774 No Hit GCCCTCGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGACCACCCGACTGCTCTTC 27 0.137209065961988 No Hit GCCCTCGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCGCGCA 26 0.1321272487041366 No Hit GCCCTCGGGGGGGCTGGAGAGATGGCTCAGTGGTTAAGAGCACTGACTGCTCTTCC 25 0.1270454314462852 No Hit GCCCTCGGGGACTTAAGCCAGCACTGCTGTGCGGGGCTCTGGCTGCGCCTGGTCTG 23 0.11688179693058237 No Hit GCCCTCGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGCCATG 21 0.10671816241487955 No Hit GCCCTCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCTGACTGCTCTTCC 20 0.10163634515702816 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0 0.0 0.0 0.0 0.0 11 0.0 0.0 0.0 0.0 0.0 12 0.0050818172578514075 0.0 0.0 0.0 0.0 13 0.0050818172578514075 0.0 0.0 0.0 0.0 14 0.0050818172578514075 0.0 0.0 0.0 0.0 15 0.010163634515702815 0.0 0.0 0.0 0.0 16 0.015245451773554223 0.0 0.0 0.0 0.0 17 0.02032726903140563 0.0 0.0 0.0 0.0 18 0.030490903547108445 0.0 0.0 0.0 0.0 19 0.030490903547108445 0.0 0.0 0.0 0.0 20 0.04065453806281126 0.0 0.0 0.0 0.0 21 0.04573635532066267 0.0 0.0 0.0 0.0 22 0.04573635532066267 0.0 0.0 0.0 0.0 23 0.06098180709421689 0.0 0.0 0.0 0.0 24 0.06098180709421689 0.0 0.0 0.0 0.0 25 0.0711454416099197 0.0 0.0 0.0 0.0 26 0.09655452789917675 0.0 0.0 0.0 0.0 27 0.10671816241487957 0.0 0.0 0.0 0.0 28 0.10671816241487957 0.0 0.0 0.0 0.0 29 0.11179997967273096 0.0 0.0 0.0 0.0 30 0.11179997967273096 0.0 0.0 0.0 0.0 31 0.11179997967273096 0.0 0.0 0.0 0.0 32 0.12196361418843378 0.0 0.0 0.0 0.0 33 0.12196361418843378 0.0 0.0 0.0 0.0 34 0.12196361418843378 0.0 0.0 0.0 0.0 35 0.12196361418843378 0.0 0.0 0.0 0.0 36 0.12196361418843378 0.0 0.0 0.0 0.0 37 0.12196361418843378 0.0 0.0 0.0 0.0 38 0.12196361418843378 0.0 0.0 0.0 0.0 39 0.1270454314462852 0.0 0.0 0.0 0.0 40 0.1270454314462852 0.0 0.0 0.0 0.0 41 0.1321272487041366 0.0 0.0 0.0 0.0 42 0.1422908832198394 0.0 0.0 0.0 0.0 43 0.1422908832198394 0.0 0.0 0.0 0.0 44 0.14737270047769083 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGGAGAA 15 0.008147609 50.246788 37 CGCCACC 15 0.008147609 50.246788 27 AGAACCT 15 0.008147609 50.246788 40 CCACTGA 15 0.008147609 50.246788 40 CCCAAGC 20 3.9844945E-4 50.246788 27 CCCCGAC 20 3.9844945E-4 50.246788 41 CCCTGAC 15 0.008147609 50.246788 40 ACCCAAG 20 3.9844945E-4 50.246788 26 CCCCCGA 20 3.9844945E-4 50.246788 40 GCCGTTA 15 0.008147609 50.246788 29 AGCCGTT 15 0.008147609 50.246788 28 AGACCAC 20 3.9844945E-4 50.246788 37 CAAGCGG 20 3.9844945E-4 50.246788 29 GGTTGGA 15 0.008231033 50.11795 32 GTGCAAT 15 0.008231033 50.11795 49 CGTTAAA 15 0.008231033 50.11795 31 TGCAATC 20 4.0355543E-4 50.11795 50 TGCGTGC 15 0.008231033 50.11795 46 TAAGACC 20 4.0355543E-4 50.11795 35 AACCTGC 20 4.0355543E-4 50.11795 42 >>END_MODULE