FastQCFastQC Report
Fri 17 Jun 2016
SRR1526180_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526180_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences65305
Sequences flagged as poor quality0
Sequence length56
%GC56

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC3520.5390092642217288No Hit
AAGCGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCCCCCGACTGCTCTTCC2540.3889441849781793No Hit
AAGCGGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTC1690.2587856978791823No Hit
AAGCGGGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGC1320.2021284740831483No Hit
AAGCGGGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAG990.15159635556236123No Hit
AAGCGGGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGCCATG800.12250210550493837No Hit
AAGCGGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGCCATGG770.11790827654850318No Hit
AAGCGGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAGGGTGT760.11637700022969145No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTCTC150.008139150.39349439
TAAGACC150.00816407650.3544935
TTAGAGC150.00821419950.27666534
GTAAGAC204.042877E-450.2766634
ATGGGTC150.00828981350.16037448
GAGTCCT150.00841698449.96774327
GGTAGCG150.00841698449.96774328
CAGTTGA150.00844259249.92939413
TAGCGAC150.00844259249.92939430
GTAGCGA150.00844259249.92939429
CAGGAAC150.00844259249.92939429
AATTCGT150.00844259249.92939413
TTCGGTA150.00844259249.92939425
CGCGTGT150.00844259249.92939425
TCGGTAG150.00844259249.92939426
ATTCGTG252.092489E-549.89110614
GGCTTCG150.00846825749.89110622
GGTAGCC150.00846825749.89110619
CGCCACG150.00846825749.89110616
AAATGAC150.00846825749.89110619
Produced by FastQC (version 0.11.5)