FastQCFastQC Report
Fri 17 Jun 2016
SRR1526179_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526179_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences96024
Sequences flagged as poor quality0
Sequence length56
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTAGGGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAG3390.3530367408147963No Hit
TTTAGGGGGGCCGGTAGTGGTGGCGCACGCCGGTAGGATTTGCTGAAGGAGGCAGA2130.22181954511372157No Hit
TTTAGGGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGC2040.21244688827793048No Hit
TTTAGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC1620.16870782304423895No Hit
TTTAGGGGGGAGGCATTCAGGCAGCGAGAGCAGAGCAGCGTAGAGCAGCACAGCTG1040.10830625676914105No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAAACAC150.00813149450.42374436
CGATTAA301.0152125E-650.15905449
CGACTCC252.0407046E-550.1327444
CAACGAT204.1209997E-450.1064546
ACGATTA204.1209997E-450.1064548
CGTGTTC204.1209997E-450.1064550
TTTCGGC150.00840646850.00156815
ACACATC150.00840646850.00156814
TTCGGCT150.00845878649.92319516
CCGCGTG150.00845878649.92319518
TCCTAAA204.2288314E-449.8450722
AGCCGTT301.0602089E-649.84506628
ACCGGGG150.00851134549.84506612
ACCATGT150.00851134549.84506625
GCGAAAC150.00851134549.84506626
CATTGCT150.00851134549.84506629
GAACTCC150.00851134549.8450669
ATTAATA150.00851134549.84506627
TGTATAA150.00851134549.84506610
CCTAAAG150.00851134549.84506623
Produced by FastQC (version 0.11.5)