##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1526179_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 96024 Sequences flagged as poor quality 0 Sequence length 56 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 38.27203615762726 39.0 38.0 40.0 35.0 40.0 2 38.18120469882529 39.0 38.0 40.0 35.0 40.0 3 37.699897942181124 39.0 38.0 40.0 35.0 40.0 4 36.71795592768475 39.0 38.0 40.0 32.0 40.0 5 37.72248604515538 39.0 38.0 40.0 35.0 40.0 6 37.752301507956346 39.0 38.0 40.0 35.0 40.0 7 38.078261684578855 39.0 38.0 40.0 35.0 40.0 8 37.97571440473215 39.0 38.0 40.0 35.0 40.0 9 37.90708572856786 39.0 38.0 40.0 35.0 40.0 10 37.05534033158377 39.0 36.0 40.0 33.0 40.0 11 35.80914146463384 38.0 35.0 39.0 29.0 40.0 12 34.32518953594935 38.0 33.0 39.0 22.0 40.0 13 34.18968174623011 38.0 33.0 39.0 22.0 40.0 14 34.24916687494793 38.0 33.0 39.0 22.0 40.0 15 34.212280263267516 38.0 33.0 39.0 22.0 40.0 16 33.64334957927185 38.0 32.0 39.0 21.0 40.0 17 33.58010497375656 38.0 31.0 39.0 21.0 40.0 18 32.99492835124552 37.0 30.0 39.0 18.0 40.0 19 34.018172540198286 38.0 32.0 39.0 24.0 40.0 20 34.000551945347 37.0 32.0 39.0 24.0 40.0 21 34.70413646588353 38.0 33.0 39.0 25.0 40.0 22 34.437057402316086 38.0 33.0 39.0 24.0 40.0 23 34.98056735816046 38.0 34.0 39.0 26.0 40.0 24 34.52974256435891 38.0 33.0 39.0 24.0 40.0 25 34.83559318503708 38.0 33.0 39.0 26.0 40.0 26 35.356942014496376 38.0 35.0 39.0 28.0 40.0 27 35.28063817378989 38.0 35.0 39.0 27.0 40.0 28 35.269745063734064 38.0 35.0 39.0 28.0 40.0 29 35.044166041822876 38.0 35.0 39.0 27.0 40.0 30 34.911761642922606 38.0 34.0 39.0 27.0 40.0 31 35.19673206698325 38.0 35.0 39.0 28.0 40.0 32 34.862628092976756 38.0 35.0 39.0 27.0 40.0 33 35.01592310255769 38.0 35.0 39.0 27.0 40.0 34 34.81090144130634 38.0 34.0 39.0 27.0 40.0 35 34.78552236940765 38.0 35.0 39.0 27.0 40.0 36 34.150306173456634 37.0 33.0 39.0 27.0 40.0 37 33.88496625843539 37.0 33.0 39.0 26.0 40.0 38 33.609087311505455 37.0 32.0 39.0 25.0 40.0 39 33.101870365741895 36.0 32.0 38.0 23.0 40.0 40 29.798737815546115 35.0 25.0 38.0 8.0 40.0 41 26.606213030075814 32.0 15.0 38.0 5.0 39.0 42 30.632404398900274 35.0 29.0 38.0 10.0 39.0 43 29.121771640423226 34.0 26.0 38.0 4.0 39.0 44 29.067983004248937 34.0 26.0 38.0 4.0 39.0 45 30.247448554528034 34.0 28.0 38.0 4.0 39.0 46 29.77201532950096 35.0 27.0 38.0 4.0 40.0 47 29.35532783470799 35.0 26.0 38.0 4.0 40.0 48 29.38980879780055 35.0 26.0 38.0 4.0 40.0 49 26.887778055486127 33.0 15.0 38.0 4.0 39.0 50 28.946513371657087 34.0 25.0 38.0 4.0 40.0 51 29.274452220278263 35.0 27.0 39.0 4.0 40.0 52 28.98497250687328 35.0 26.0 39.0 4.0 40.0 53 28.563567441472966 35.0 24.0 38.0 4.0 40.0 54 27.56069316004332 34.0 18.0 38.0 4.0 40.0 55 27.954573856535866 34.0 23.0 38.0 4.0 40.0 56 26.719809630925603 33.0 17.0 38.0 4.0 39.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1 1 0.10369359543989987 1 2 0.2984767375628863 1 3 1.058597861701358 1 4 1.8006454501957094 1 5 0.5491876853976194 1 6 0.24048533816354478 1 7 0.26889409234548367 1 8 0.41241431065085266 1 9 0.43950365602795216 1 10 0.2792574773117664 1 11 -4.147207192017895 1 12 -13.109729244297746 1 13 -7.7005101921643515 1 14 -6.140139179292667 1 15 -7.575169458255697 1 16 -23.599728462773903 1 17 -5.548588078152115 1 18 -14.63451908311465 1 19 -6.549612219514554 1 20 -6.289015959832174 1 21 -6.4387404588331165 1 22 -2.6185919451500688 1 23 -3.431768526351963 1 24 -3.875561593596661 1 25 -3.200526326967296 1 26 1.2789252525854522 1 27 1.850490063816352 1 28 1.1675480925910904 1 29 1.7964535491903533 1 30 1.736246883796639 1 31 1.7042115396598732 1 32 1.9898968956583047 1 33 1.655703327901847 1 34 0.8829365776206473 1 35 1.6076743537539713 1 36 1.802872267556637 1 37 1.5703715467303851 1 38 1.7753347939520765 1 39 2.1169733843938516 1 40 5.365940504173089 1 41 -9.82102577454702 1 42 -9.923936221061034 1 43 -16.42277816149988 1 44 -21.626176284749338 1 45 -10.832665598273742 1 46 -17.284416337988958 1 47 -23.052887971946635 1 48 -14.274073186374606 1 49 -19.705175705776576 1 50 -13.482993712934464 1 51 -10.683830873515657 1 52 -10.43889835352865 1 53 -10.158133737198956 1 54 -9.216811265334695 1 55 -9.590925539164129 1 56 -5.006872017739131 2 1 0.8631864347514195 2 2 1.0085673958206698 2 3 1.8195359775862627 2 4 2.133847394749644 2 5 1.1089695800238175 2 6 0.9740945879336138 2 7 0.7979967069821896 2 8 0.7990914884198759 2 9 0.7515520070987662 2 10 0.7247311338614963 2 11 0.17456392872839643 2 12 -2.228439006484056 2 13 -1.5052664659785648 2 14 2.721902602339185 2 15 2.4084726387092203 2 16 2.385818771492289 2 17 2.822059668530315 2 18 -2.214009294650584 2 19 2.0516885294014884 2 20 2.1370121573666196 2 21 2.065398287717926 2 22 2.490657824919566 2 23 1.7575627047609004 2 24 1.1432270018572908 2 25 1.4268550946592953 2 26 1.245224030669803 2 27 1.3153435653273249 2 28 1.4026683133242415 2 29 1.7616355226498186 2 30 1.661618057098444 2 31 1.0580415225793303 2 32 1.5142695142366804 2 33 1.3728232858574358 2 34 0.8542281293221308 2 35 0.7382091060861242 2 36 1.8909684441431551 2 37 1.805294684294438 2 38 1.4314246638771806 2 39 2.288895602236451 2 40 3.792691259658575 2 41 -7.035386568135248 2 42 -7.862052105964462 2 43 -14.540436813451006 2 44 -14.179914295523218 2 45 -7.115414251538752 2 46 -11.366731408281954 2 47 -16.983580390814062 2 48 -9.03724491150928 2 49 -12.274877453247342 2 50 -8.135864557764314 2 51 -3.489047008057767 2 52 -2.94862112320412 2 53 -3.017744826411935 2 54 -1.6471752122536287 2 55 -2.2044454445642074 2 56 0.35845448337767394 3 1 1.0319018278854202 3 2 1.1642152363522271 3 3 1.97518381811782 3 4 2.6146890337308193 3 5 1.4522143197801896 3 6 1.4281366698826687 3 7 0.6010974821759874 3 8 1.103576538253762 3 9 1.0288499030013156 3 10 1.1502017872391264 3 11 2.3243424447859766 3 12 3.807219908244626 3 13 3.152153246092311 3 14 3.9556235325717424 3 15 2.8803995490082244 3 16 4.330780011802368 3 17 4.348748483591223 3 18 4.961848955626273 3 19 3.722784874916435 3 20 3.760378713291317 3 21 3.176029516953811 3 22 3.111311202549686 3 23 3.3814275995560266 3 24 3.2733488180256174 3 25 3.2699337214588553 3 26 2.881104429340901 3 27 2.9785772308865717 3 28 2.693698213656468 3 29 2.510860328851372 3 30 2.834430903167103 3 31 2.1705553653257326 3 32 2.763383578467021 3 33 2.584395821848581 3 34 1.7300310086133805 3 35 2.3196598259089356 3 36 2.872363792980366 3 37 3.2033013288127066 3 38 3.2987004113854894 3 39 3.9480871858466386 3 40 7.002381182139196 3 41 8.341080762983243 3 42 4.832964505331219 3 43 -0.6681776772383792 3 44 3.0747922382530817 3 45 4.974840454995025 3 46 3.249603032471093 3 47 -3.0180211438594675 3 48 2.462367491591497 3 49 5.1949453595987265 3 50 3.1352871587362365 3 51 4.07512796425674 3 52 3.471755399497983 3 53 3.4720115412514083 3 54 4.490089461057558 3 55 3.9855877780271562 3 56 4.1092296771761205 4 1 0.6873963333799225 4 2 1.0713580934950855 4 3 1.7207882137222157 4 4 1.77952419856598 4 5 1.0824340999999649 4 6 0.9863784281244321 4 7 0.8549436360221421 4 8 1.0337963184735415 4 9 0.8409378150892266 4 10 1.3062457432830783 4 11 2.7171995876431225 4 12 4.10721990824463 4 13 4.301054344993403 4 14 4.2825466094948155 4 15 3.3567731753818535 4 16 4.253856934879288 4 17 4.5806166154593555 4 18 5.5178929116702236 4 19 3.811795863927429 4 20 3.1735655264781286 4 21 2.3496558905801805 4 22 2.9514210926595794 4 23 1.6116473797758104 4 24 2.775546620223416 4 25 2.1303732818984145 4 26 2.1536319018683727 4 27 1.9313244836338228 4 28 2.040950960909214 4 29 2.407014175005216 4 30 1.6855298042660039 4 31 1.1837421785125457 4 32 2.277119842203284 4 33 1.5278024152551737 4 34 0.7064046349870097 4 35 0.5377916940408056 4 36 2.63390225451883 4 37 2.9890156145269913 4 38 3.436612499297574 4 39 3.469515757275211 4 40 6.521611951369962 4 41 9.965806037708521 4 42 6.32417329654001 4 43 7.347756388695682 4 44 6.95061641407726 4 45 6.052862433017005 4 46 6.421581054449113 4 47 5.642418416580092 4 48 6.127752106976111 4 49 9.013077227730598 4 50 6.680891554340636 4 51 5.643809282938054 4 52 5.4442828720254575 4 53 5.233000552240419 4 54 5.6774520984201935 4 55 3.9603130527524293 4 56 4.066921984868429 5 1 0.9731598236210246 5 2 1.0295670487189668 5 3 1.9450132424248565 5 4 2.444966219231887 5 5 1.136969117221554 5 6 1.1903967978144436 5 7 0.9100527060565824 5 8 0.9301223804712464 5 9 0.7672294339181605 5 10 0.9335706571751601 5 11 2.254513020478946 5 12 4.325130356005822 5 13 3.8761404529153367 5 14 4.381742935556815 5 15 4.747243898688396 5 16 4.7457053849366915 5 17 4.800134411096558 5 18 5.463128273323498 5 19 4.12683604762433 5 20 3.9038755149970683 5 21 3.4591851672736382 5 22 3.3003303923151464 5 23 2.9751376208779874 5 24 3.4119415685586674 5 25 3.085711973058004 5 26 2.2113816148419687 5 27 2.7137584675603392 5 28 1.9092632456394085 5 29 2.2728006273588335 5 30 2.2999959351500436 5 31 1.0409178386732805 5 32 2.184812149895592 5 33 1.7962295105479384 5 34 1.8312037165025075 5 35 1.4600649431797237 5 36 2.464901106413201 5 37 2.323343972735948 5 38 2.434201477483569 5 39 3.3142918766781904 5 40 6.701634913482479 5 41 8.41815965424124 5 42 4.720384547975144 5 43 6.223186928305328 5 44 5.356242131643278 5 45 4.872495039216773 5 46 4.5691126273538245 5 47 4.627607854008339 5 48 4.748188387113885 5 49 7.3756489843322015 5 50 5.072280762147752 5 51 3.946449925877207 5 52 4.546808704402036 5 53 4.526915592424118 5 54 4.591688608179091 5 55 4.452965176747838 5 56 4.914453557773136 6 1 0.9378926112955597 6 2 1.3810355128499268 6 3 2.054907320421968 6 4 2.7331222134543225 6 5 1.5303249188585255 6 6 1.3774454256430388 6 7 1.4269039337888927 6 8 1.222009717977265 6 9 1.4362231748907135 6 10 1.9829206812483449 6 11 2.9430060392560264 6 12 4.544316682438179 6 13 4.356761541023182 6 14 4.460462242249157 6 15 4.945606922280113 6 16 5.190457431157206 6 17 5.2570434144668 6 18 5.610696881893553 6 19 4.284996856483261 6 20 4.292175948314359 6 21 3.5108221436819207 6 22 3.599560050475958 6 23 3.562764005085981 6 24 4.102841905583219 6 25 3.6584129841316653 6 26 3.0963118026127887 6 27 2.675121009688411 6 28 3.3874770154997833 6 29 3.6608603288513706 6 30 3.2298226082362262 6 31 2.52815905196168 6 32 3.21707021441172 6 33 2.3668843011112486 6 34 2.3307222528530147 6 35 2.9823326369688417 6 36 3.3110734703997196 6 37 3.2696607758173144 6 38 3.3639077846573784 6 39 3.98725769275908 6 40 6.786252149881129 6 41 4.014813481877255 6 42 5.492411509939515 6 43 7.223066562392955 6 44 5.305206984796861 6 45 5.494425708451246 6 46 5.968727456434223 6 47 5.694651667200439 6 48 6.1381739432044 6 49 8.038263331948961 6 50 5.623075177169415 6 51 5.435496627851212 6 52 5.612769224382777 6 53 5.119476979039426 6 54 6.247923562440047 6 55 5.73835275498568 6 56 5.281810322337414 7 1 1.0023431751560352 7 2 1.1063174430885852 7 3 1.9416762687566163 7 4 2.356384736402127 7 5 1.3260238435897094 7 6 1.0506686094877793 7 7 0.9246747179483492 7 8 1.1643779319819814 7 9 0.9489428182161816 7 10 1.1346384887489975 7 11 2.6521589372366208 7 12 3.7242930789763378 7 13 4.088796683955259 7 14 4.093428410620518 7 15 4.74445297525677 7 16 5.133219036192614 7 17 4.946657891256734 7 18 5.1527897221767915 7 19 4.158324944602846 7 20 3.7100883532448563 7 21 2.7018134890792425 7 22 2.7069558018528284 7 23 3.0406029770240863 7 24 2.7594115357956497 7 25 2.8679592731429437 7 26 1.948003384044732 7 27 1.766846685009682 7 28 1.387542580671564 7 29 2.083624556493646 7 30 1.7294947823773228 7 31 1.6186389890191109 7 32 2.30269832875738 7 33 1.1867767742295285 7 34 1.401401508032663 7 35 1.1311000117393633 7 36 1.3748028173706146 7 37 1.42885301290098 7 38 1.9513717238128976 7 39 1.6475645377630102 7 40 4.812543783765207 7 41 -1.2136811417786575 7 42 3.3472502196169316 7 43 5.2498827176512854 7 44 4.92728933465262 7 45 3.5410425455873593 7 46 4.674864356512906 7 47 4.875939367174212 7 48 4.696920337119948 7 49 7.037279854372244 7 50 5.253812129700233 7 51 4.7660106587979705 7 52 4.763044598104255 7 53 5.0344389512397925 7 54 5.4893345249367655 7 55 5.336110426110782 7 56 5.686871953598885 8 1 1.2005559010849467 8 2 1.2540027215942615 8 3 2.1806737826986975 8 4 2.65034428756789 8 5 1.5271257719643643 8 6 1.2073574491034478 8 7 1.2010974821759888 8 8 1.2611326185371112 8 9 1.1155086987510217 8 10 1.6522088710643885 8 11 2.4940590917753553 8 12 4.671682718161982 8 13 4.4156125140970275 8 14 5.018846673067607 8 15 4.604897778051907 8 16 5.387804805190797 8 17 5.417933843685674 8 18 5.627492403087899 8 19 4.468947802897546 8 20 4.1699418773999355 8 21 3.365994097827482 8 22 3.811370234426903 8 23 3.086091117855908 8 24 3.7166782159004654 8 25 3.5205830721082023 8 26 2.958081997227559 8 27 2.673854680709475 8 28 3.3215612597013333 8 29 3.0327611552976492 8 30 3.1751038665673335 8 31 2.663589603814515 8 32 2.098035290391458 8 33 2.6771349009512946 8 34 1.984281303772768 8 35 2.5599785980458876 8 36 2.2161654458729316 8 37 2.036123052543523 8 38 2.188487896627521 8 39 2.835631459754545 8 40 4.59370349618878 8 41 -3.4588602051395405 8 42 2.843117988211972 8 43 4.974266242478269 8 44 5.160978778985076 8 45 3.165513418395257 8 46 3.642814366591516 8 47 3.7846943224923635 8 48 4.189226995723729 8 49 6.084850908595186 8 50 3.9349919993501707 8 51 4.137642722226044 8 52 3.715675116144972 8 53 4.587418861204185 8 54 5.2816479026159975 8 55 4.238952595028337 8 56 4.822866040812485 9 1 0.9070249805947768 9 2 1.1885994728054285 9 3 1.8702577097434414 9 4 2.451142235701262 9 5 1.1679778665782194 9 6 0.7951317437742986 9 7 0.9252354132104728 9 8 1.0865814643621334 9 9 1.0613622182722509 9 10 1.19232001384011 9 11 2.8120271738500193 9 12 4.269288873761866 9 13 3.8509217189987126 9 14 4.665968360157947 9 15 4.101335509599359 9 16 4.70491794283685 9 17 4.865497252064131 9 18 5.122932699468635 9 19 3.986331672945994 9 20 3.98377772806964 9 21 3.105093556362675 9 22 3.44160676905215 9 23 3.262708387733369 9 24 3.129260148074877 9 25 2.7462884012618076 9 26 1.6830748726906535 9 27 1.9704491520688379 9 28 2.5087228441983385 9 29 1.6608603288513706 9 30 2.2787658785365608 9 31 1.166368173207502 9 32 1.7710190464473143 9 33 1.3045928661835546 9 34 1.1457945554114133 9 35 0.9167041608843078 9 36 1.765467241256232 9 37 1.783843200733891 9 38 1.7610156823214496 9 39 1.6488261021027952 9 40 3.6816915269667803 9 41 2.9817211570719167 9 42 3.261043323065209 9 43 4.657438086308421 9 44 4.297698642193971 9 45 3.165727154502413 9 46 3.493199091584394 9 47 3.896264570426247 9 48 3.4037468019363253 9 49 6.667851763539616 9 50 3.8010507055342657 9 51 3.7650294420892507 9 52 4.059932739399461 9 53 3.5619130190839208 9 54 4.23540965810189 9 55 4.222779896253755 9 56 5.028195194417503 10 1 0.867616113599702 10 2 0.9948875052597899 10 3 1.8478728937480753 10 4 2.0886386135627504 10 5 0.9450714626373298 10 6 1.1508257455129254 10 7 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26 1.9215806198170924 117 27 2.0357200880294286 117 28 1.7246505946088462 117 29 0.9584793764704216 117 30 1.6749070936432915 117 31 1.8157934605638246 117 32 2.0657645308479715 117 33 1.0915386789914336 117 34 1.8633643419467134 117 35 1.4339455401946495 117 36 2.2223637929803672 117 37 2.486634662146045 117 38 2.2010813637664413 117 39 2.9838014715609233 117 40 6.909524039282047 117 41 4.952985524888007 117 42 -0.48608311371640056 117 43 -5.861034820095522 117 44 -7.079969666508823 117 45 0.41531664547121494 117 46 -2.8623017294336677 117 47 -3.3894497152880376 117 48 -4.647156317932314 117 49 -7.590768926115558 117 50 -4.18376046031138 117 51 -4.682014892886119 117 52 -4.906816029073443 117 53 -5.2184646492247815 117 54 -12.612291491323395 117 55 -5.547745555306179 117 56 -4.62410365615721 118 1 -0.2768830653822292 118 2 -0.09300972259686802 118 3 0.6834761005480345 118 4 1.439647982827701 118 5 0.2570583263483357 118 6 0.18018921503866636 118 7 -0.32763815000792107 118 8 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28 1.0305752900684269 119 29 2.0794649800141585 119 30 2.0724707702767375 119 31 1.5619174915715774 119 32 -3.7454204082439446 119 33 1.5666217354698375 119 34 0.5674064238957754 119 35 1.2653408890318616 119 36 1.4142242580966453 119 37 1.4564574749921135 119 38 1.3151455941097439 119 39 2.5834692456473007 119 40 4.598892810046166 119 41 1.305698703182582 119 42 -3.8620521059644624 119 43 -10.61020425531147 119 44 -9.187666233507716 119 45 -5.181305724406968 119 46 -15.362855439289705 119 47 -14.475828452829568 119 48 -12.448097624687573 119 49 -13.026815437743466 119 50 -11.236639751562766 119 51 -8.880519876274821 119 52 -8.836218022428927 119 53 -9.839450252768525 119 54 -12.875857382796264 119 55 -10.599794281773509 119 56 -7.133793578637832 120 1 -0.5768283308447408 120 2 -0.7334038112668182 120 3 0.36327905621305945 120 4 1.0242128432546238 120 5 -0.32476980720393556 120 6 -0.3972601555141537 120 7 -0.6560453749668653 120 8 -0.8480107633335408 120 9 -0.43781676366535294 120 10 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48 -14.635251556027551 120 49 -18.364578449925084 120 50 -14.116300142851063 120 51 -12.551062511933738 120 52 -12.44253031478773 120 53 -11.817670998431131 120 54 -15.763085142117047 120 55 -13.706475714036337 120 56 -11.03283381488737 >>END_MODULE >>Per sequence quality scores pass #Quality Count 4 248.0 5 34.0 6 62.0 7 111.0 8 213.0 9 234.0 10 179.0 11 133.0 12 145.0 13 140.0 14 173.0 15 226.0 16 264.0 17 383.0 18 455.0 19 596.0 20 757.0 21 920.0 22 1206.0 23 1415.0 24 1597.0 25 1846.0 26 2064.0 27 2275.0 28 2580.0 29 2988.0 30 3573.0 31 4293.0 32 4930.0 33 5702.0 34 6398.0 35 7867.0 36 9846.0 37 12358.0 38 12153.0 39 7656.0 40 4.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 0.0 0.0 100.0 0.0 2 0.0 0.0 100.0 0.0 3 0.0 0.0 100.0 0.0 4 0.0 100.0 0.0 0.0 5 100.0 0.0 0.0 0.0 6 100.0 0.0 0.0 0.0 7 99.13563275847704 0.6967008247938016 0.1364242272765142 0.03124218945263684 8 98.72948429559277 1.0393235024577188 0.1395484462217779 0.09164375572773473 9 92.62684328917771 4.456177622261102 1.888069649254353 1.0289094393068399 10 38.36645005415313 45.19286844955428 8.331250520703158 8.109430975589436 11 33.89673414979588 28.292926768307925 21.037448971090562 16.772890110805633 12 34.28517870532367 15.733566608347912 23.093185037074065 26.888069649254355 13 25.98652350055718 16.93310698924171 26.740540934606692 30.339828575594414 14 24.345208960353247 20.468012871916102 28.580652551992753 26.606125615737895 15 26.46343627046828 21.10019933416128 27.81703002536031 24.619334370010122 16 30.219471018570626 23.53425456970758 24.302298922445235 21.94397548927656 17 28.51266350079147 23.768016329251022 25.22181954511372 22.497500624843788 18 25.760226610014165 24.943764058985256 25.312421894526366 23.983587436474217 19 27.310538934639844 27.168768893985195 22.89064943187741 22.63004273949755 20 27.903486372400856 26.28172390432018 23.854291521184013 21.96049820209495 21 25.537365658585355 23.48475381154711 28.35436974089811 22.623510788969426 22 26.212196950762312 23.370199116887445 27.034907939681744 23.382695992668502 23 25.929975839373494 24.324127301507957 27.48271265516954 22.263184203949013 24 25.576430401366352 25.260877715523527 26.330424277769676 22.83226760534044 25 27.611955842532804 25.317642157883775 25.547802541137266 21.522599458446155 26 23.929345109148475 24.90105815697384 27.72246292284619 23.447133811031495 27 23.720421140414885 22.62900031272803 28.965912644636717 24.684665902220367 28 23.682372199226368 23.847108256612902 29.68689722763812 22.78362231652261 29 25.306011896700802 23.190232621128622 30.19595178815122 21.307803694019356 30 23.008019997916882 21.413394438079365 34.27247161753984 21.30611394646391 31 22.722916666666666 22.698958333333334 34.047916666666666 20.530208333333334 32 25.33746104214224 20.758414376100983 34.884351188800984 19.019773392955795 33 24.429264222766854 20.56526046826928 36.69291338582677 18.312561923137093 34 20.674925245621527 22.43464852418708 40.24963273981309 16.640793490378304 35 19.322171634560288 24.541845951887314 40.1841992852901 15.951783128262296 36 20.995062191386964 24.904681542596414 37.170031460299604 16.930224805717025 37 21.397051920007513 23.644102275216724 37.965136293931714 16.993709510844052 38 22.376088727363868 23.906050922154687 37.3540530943877 16.36380725609374 39 21.067020044450473 21.061802852760415 38.11888936423302 19.75228773855609 40 16.362930962167603 16.92811005788105 35.48674607834913 31.222212901602216 41 14.265155414561384 16.319842011828104 33.588243326995595 35.82675924661491 42 19.807297260659652 22.350598025005475 38.977465875556575 18.864638838778298 43 17.39851994113165 18.71262016345361 38.551462836744705 25.337397058670035 44 17.71520060275005 19.137313995102655 37.720014231598334 25.427471170548966 45 20.31535338989241 22.54536726878085 38.806624299533546 18.332655041793195 46 18.4571249739203 23.425829334446068 37.93135823075318 20.185687460880448 47 17.797943385807905 23.623975078920225 38.547451111134265 20.03063042413761 48 19.996244836650394 23.783744315099927 38.763716777235366 17.456294071014312 49 17.574996073504003 18.9644521229255 34.048479137217946 29.412072666352547 50 18.967872449139197 23.731217218114892 38.29132733396594 19.009582998779965 51 19.325694828864773 24.43938460252969 37.58708208432993 18.647838484275614 52 18.495271658099696 23.946158417699742 38.61600859129818 18.94256133290238 53 18.79100818859855 23.07620090752621 39.52433109059615 18.60845981327909 54 16.861672702981974 23.56981076934317 36.2460729159056 23.322443611769252 55 19.44200901803607 24.03870240480962 37.67848196392786 18.84080661322645 56 20.539412593249516 24.494757159997913 36.310710000521674 18.655120246230894 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.5 4 8.5 5 16.0 6 39.5 7 63.0 8 110.5 9 158.0 10 214.5 11 271.0 12 323.0 13 392.0 14 409.0 15 503.0 16 597.0 17 712.0 18 827.0 19 874.5 20 922.0 21 1150.5 22 1675.5 23 1972.0 24 2228.5 25 2485.0 26 2804.0 27 3123.0 28 3399.5 29 3775.0 30 3874.0 31 3607.5 32 3341.0 33 3029.5 34 2718.0 35 2565.0 36 2412.0 37 2343.5 38 2366.0 39 2457.0 40 2500.5 41 2544.0 42 2689.0 43 2834.0 44 3126.5 45 3419.0 46 3496.5 47 3599.0 48 3624.0 49 3786.0 50 3948.0 51 4106.5 52 4265.0 53 4371.0 54 4547.0 55 4617.0 56 4744.0 57 4871.0 58 5166.0 59 5461.0 60 5235.0 61 5009.0 62 4651.0 63 3816.0 64 3339.0 65 2758.0 66 2177.0 67 1846.0 68 1515.0 69 1308.0 70 1101.0 71 892.5 72 557.0 73 430.0 74 340.0 75 250.0 76 187.5 77 125.0 78 99.0 79 46.0 80 19.0 81 16.5 82 14.0 83 9.5 84 5.0 85 3.5 86 2.0 87 1.5 88 0.5 89 0.0 90 1.0 91 2.0 92 1.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.005207031575439473 14 0.0010414063150878945 15 0.21348829459301844 16 0.06873281679580105 17 0.0 18 0.0 19 0.0978921936182621 20 0.08227109889194369 21 0.0 22 0.0 23 0.0 24 0.002082812630175789 25 0.004165625260351578 26 0.008331250520703156 27 0.0978921936182621 28 0.1176789136049321 29 0.032283595767724736 30 0.014579688411230527 31 0.024993751562109475 32 0.09060234941264685 33 0.14475547779721737 34 0.04478047154877947 35 0.042697658918603686 36 0.03124218945263684 37 0.17183204198950264 38 0.2811797050737316 39 0.19474298092143633 40 0.683162542697659 41 0.8612430225776889 42 0.13017578938598684 43 0.22598517037407312 44 0.4811297175706074 45 0.20307423144213946 46 0.17079063567441474 47 0.042697658918603686 48 0.16245938515371156 49 0.5404898775306174 50 0.13017578938598684 51 0.2926351745396984 52 0.1176789136049321 53 0.16558360409897527 54 0.22390235774389736 55 0.22494376405898525 56 0.18641173040073314 >>END_MODULE >>Sequence Length Distribution pass #Length Count 56 96024.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 75.14371407148212 #Duplication Level Percentage of deduplicated Percentage of total 1 86.12035035201508 64.71402982587686 2 8.21830478407894 12.35107889694243 3 2.6484284051222353 5.9703824043989 4 1.1544431509507178 3.469965841872865 5 0.5931592660347026 2.2286095142880944 6 0.37557514274627196 1.6933266683329167 7 0.2106546926104551 1.10805631925352 8 0.14690393037308053 0.8831125551945348 9 0.08731082654249127 0.5904773806548363 >10 0.4254670436276956 5.235149545946847 >50 0.012472975220355896 0.691493793218362 >100 0.006929430677975498 1.0643172540198282 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source TTTAGGGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAG 339 0.3530367408147963 No Hit TTTAGGGGGGCCGGTAGTGGTGGCGCACGCCGGTAGGATTTGCTGAAGGAGGCAGA 213 0.22181954511372157 No Hit TTTAGGGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGC 204 0.21244688827793048 No Hit TTTAGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC 162 0.16870782304423895 No Hit TTTAGGGGGGAGGCATTCAGGCAGCGAGAGCAGAGCAGCGTAGAGCAGCACAGCTG 104 0.10830625676914105 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0010414063150878947 0.0 0.0 0.0 0.0 9 0.003124218945263684 0.0 0.0 0.0 0.0 10 0.014579688411230526 0.0 0.0 0.0 0.0 11 0.01562109472631842 0.0 0.0 0.0 0.0 12 0.022910938931933682 0.0 0.0 0.0 0.0 13 0.026035157877197367 0.0 0.0 0.0 0.0 14 0.02707656419228526 0.0 0.0 0.0 0.0 15 0.028117970507373156 0.0 0.0 0.0 0.0 16 0.03332500208281263 0.0 0.0 0.0 0.0 17 0.03749062734316421 0.0 0.0 0.0 0.0 18 0.038532033658252106 0.0 0.0 0.0 0.0 19 0.045821877863867364 0.0 0.0 0.0 0.0 20 0.04686328417895526 0.0 0.0 0.0 0.0 21 0.04790469049404315 0.0 0.0 0.0 0.0 22 0.05519453469965842 0.0 0.0 0.0 0.0 23 0.05936015996001 0.0 0.0 0.0 0.0 24 0.06352578522036158 0.0 0.0 0.0 0.0 25 0.06560859785053737 0.0 0.0 0.0 0.0 26 0.07393984837124053 0.0 0.0 0.0 0.0 27 0.07602266100141632 0.0 0.0 0.0 0.0 28 0.08122969257685579 0.0 0.0 0.0 0.0 29 0.08643672415229527 0.0 0.0 0.0 0.0 30 0.09060234941264683 0.0 0.0 0.0 0.0 31 0.09164375572773473 0.0 0.0 0.0 0.0 32 0.09476797467299841 0.0 0.0 0.0 0.0 33 0.0968507873031742 0.0 0.0 0.0 0.0 34 0.0968507873031742 0.0 0.0 0.0 0.0 35 0.09893359993334999 0.0 0.0 0.0 0.0 36 0.10101641256352578 0.0 0.0 0.0 0.0 37 0.10205781887861368 0.0 0.0 0.0 0.0 38 0.10309922519370157 0.0 0.0 0.0 0.0 39 0.10414063150878947 0.0 0.0 0.0 0.0 40 0.10622344413896526 0.0 0.0 0.0 0.0 41 0.10622344413896526 0.0 0.0 0.0 0.0 42 0.10726485045405315 0.0 0.0 0.0 0.0 43 0.10830625676914105 0.0 0.0 0.0 0.0 44 0.11143047571440473 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TAAACAC 15 0.008131494 50.423744 36 CGATTAA 30 1.0152125E-6 50.159054 49 CGACTCC 25 2.0407046E-5 50.13274 44 CAACGAT 20 4.1209997E-4 50.10645 46 ACGATTA 20 4.1209997E-4 50.10645 48 CGTGTTC 20 4.1209997E-4 50.10645 50 TTTCGGC 15 0.008406468 50.001568 15 ACACATC 15 0.008406468 50.001568 14 TTCGGCT 15 0.008458786 49.923195 16 CCGCGTG 15 0.008458786 49.923195 18 TCCTAAA 20 4.2288314E-4 49.84507 22 AGCCGTT 30 1.0602089E-6 49.845066 28 ACCGGGG 15 0.008511345 49.845066 12 ACCATGT 15 0.008511345 49.845066 25 GCGAAAC 15 0.008511345 49.845066 26 CATTGCT 15 0.008511345 49.845066 29 GAACTCC 15 0.008511345 49.845066 9 ATTAATA 15 0.008511345 49.845066 27 TGTATAA 15 0.008511345 49.845066 10 CCTAAAG 15 0.008511345 49.845066 23 >>END_MODULE