FastQCFastQC Report
Fri 17 Jun 2016
SRR1526178_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526178_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18885
Sequences flagged as poor quality0
Sequence length56
%GC50

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTTTCGGGTCACAGATCCTCTTCTGAGATGAGTTTTTGTTCAATCCGCCCAATCT5042.66878474980143No Hit
CCTTTCGGGTCACAGATCCTCTTCTGAAGGAGATTGGGCGGATTGAACAAAAACTC3351.7738946253640455No Hit
CCTTTCGGGTCACAGATCCTCTTCTGAAGGAGATTGGGCGCATTGAACAAAAACTC1210.6407201482658195No Hit
CCTTTCGGGTCACAGATCCTCTTCTGAGATGAGTTTTTGTCCAATCCGCCCAATCT1070.5665872385491131No Hit
CCTTTCGGGAACTGTGGTAATTCTAGAGCTAATACATGCCGACGGGCGCTGACCCC620.3283028858882711No Hit
CCTTTCGGGGGGCGCTGACCCCCCTTCCCGGGGGGGGATGCGTGCATTTATCAGAT530.28064601535610273No Hit
CCTTTCGGGGGTGAGCTCCCTCCCGGCTCCGGCCGGGGGTCGGGCGCCGGCGGCTT500.2647603918453799No Hit
CCTTTCGGTCACAGATCCTCTTCTGAGATGAGTTTTTGTTCAATCCGCCCAATCTC490.25946518400847235No Hit
CCTTTCGGGTCACAGATCCTCTTCTGAGATGAGTTTTTGTTCAATCCGCCCAAAAA480.2541699761715647No Hit
CCTTTCGGGGTCTGTCCGTCCGTGGAGCCAGTCCTAGCCACATTATTGACAGTCTT430.22769393698702675No Hit
CCTTTCGGGGAACGTCTGCCCTATCAACTTTCGATGGTAGTCGCCGTGCCTACCAT430.22769393698702675No Hit
CCTTTCGGGGTGGCGGCGACGACCCATTCGAACGTCTGCCCTATCAACTTTCGATG420.22239872915011916No Hit
CCTTTCGGGTCACAGATCCTCTTCTGAGATGAGTTTTTGCTCAATCCGCCCAATCT400.21180831347630394No Hit
CCTTTCGGGTCACAGATCCTCTTCTGAAGGAGATTGGGCGGATTGACCAAAAACTC390.20651310563939632No Hit
CCTTTCGGGGGTTCCTTTGGTCGCTCGCTCCTCTCCTACTTGGATAACTGTGGTAA340.18003706645485837No Hit
CCTTTCGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAG320.16944665078104318No Hit
CCTTTCGGGATCCAAGGAAGGCAGCAGGCGCGCAAATTACCCACTCCCGACCCGGG300.15885623510722796No Hit
CCTTTCGGGTCACAGATCCTCTTCTGAAGGAGATTGGGCCGATTGAACAAAAACTC300.15885623510722796No Hit
CCTTTCGGGGGAGAGGGAGCCTGAGAAACGGCTACCACATCCAAGGAAGGCAGCAG290.15356102727032034No Hit
CCTTTCGGGTCACAGATCCTCTGAAGGAGATTGGGCGGATTGAACAAAAACTCATC270.14297061159650515No Hit
CCTTTCGGGACCCACTCCCGACCCGGGGAGGTAGTGACGAAAAATAACAATACAGG260.13767540375959755No Hit
CCTTTCGGGCTCCTACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCGAC260.13767540375959755No Hit
CCTTTCGGGAGTTATGGTTCCTTTGGTCGCTCGCTCCTCTCCTACTTGGATAACTG260.13767540375959755No Hit
CCTTTCGGGATGGTTCCTTTGGTCGCTCGCTCCTCTCCTACTTGGATAACTGTGGT250.13238019592268996No Hit
CCTTTCGGGAGTGACGAAAAATAACAATACAGGACTCTTTCGAGGCCCTGTAATTG250.13238019592268996No Hit
CCTTTCGGGGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCCATTCGAACGTC240.12708498808578236No Hit
CCTTTCGGGACTTTGAAAAAATTAGAGTGTTCAAAGCAGGCCCGAGCCGCCTGGAT210.11119936457505958No Hit
CCTTTCGGGGGGGAGGTAGTGACGAAAAATAACAATACAGGACTCTTTCGAGGCCC200.10590415673815197No Hit
CCTTTCGGGGGTGACTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGC190.10060894890124437No Hit
CCTTTCGGGTCCCCCCTCCTCTTCTGAGATGAGTTTTTGTTCCCTCCGCCCAATCT190.10060894890124437No Hit
CCTTTCGGGAACTGTGGTAATTCTAGAGCTAATACATGCCCACGGGCGCTGACCCC190.10060894890124437No Hit
CCTTTCGGGAGCCGCGGTAATTCCAGCTCCAATAGCGTATATTAAAGTTGCTGCAG190.10060894890124437No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTGTTCC309.134892E-750.6064737
CATTGAA354.4752596E-850.6064741
GGCGCAT309.134892E-750.6064737
GTTCCCT150.00791531450.6064739
GTTCCAT150.00791531450.6064739
TGTTCCC150.00791531450.6064738
TGTTCCA150.00791531450.6064738
TTCTCAA150.00791531450.6064738
GCGCATT309.134892E-750.6064738
TGGGCCG150.00791531450.6064735
CGCATTG354.4752596E-850.6064739
GCCGATT150.00791531450.6064738
GCATTGA354.4752596E-850.6064740
TCTCAAT150.00791531450.6064739
CCGATTG150.00791531450.6064739
CGATTGA150.00791531450.6064740
TTGGGCC150.00808602350.33511734
CCCGGTT252.0253136E-549.93351419
CATCCGC252.0253136E-549.93351443
GTATCAA252.0253136E-549.93351418