##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1526178_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 18885 Sequences flagged as poor quality 0 Sequence length 56 %GC 50 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 38.71337039978819 39.0 38.0 40.0 37.0 40.0 2 38.47916335716177 39.0 38.0 40.0 36.0 40.0 3 38.17590680434207 39.0 38.0 40.0 35.0 40.0 4 38.29944400317712 39.0 38.0 40.0 35.0 40.0 5 38.290177389462535 39.0 38.0 40.0 35.0 40.0 6 37.435266084193806 39.0 38.0 40.0 33.0 40.0 7 37.79528726502515 39.0 38.0 40.0 35.0 40.0 8 37.738893301562086 39.0 38.0 40.0 35.0 40.0 9 37.76176859941753 39.0 38.0 40.0 35.0 40.0 10 36.878157267672755 39.0 37.0 40.0 33.0 40.0 11 35.3559968228753 38.0 35.0 40.0 27.0 40.0 12 34.252581413820494 38.0 33.0 40.0 22.0 40.0 13 33.65046333068573 38.0 32.0 40.0 20.0 40.0 14 34.28752978554408 38.0 33.0 40.0 23.0 40.0 15 32.79925867090283 38.0 31.0 39.0 16.0 40.0 16 32.654275880328306 37.0 30.0 39.0 19.0 40.0 17 31.542441090812815 36.0 27.0 39.0 15.0 40.0 18 31.05845909451946 36.0 26.0 39.0 14.0 40.0 19 32.380090018533224 36.0 29.0 39.0 18.0 40.0 20 32.900450092666134 37.0 30.0 39.0 20.0 40.0 21 33.40058247286206 38.0 31.0 39.0 19.0 40.0 22 32.636960550701616 38.0 30.0 39.0 17.0 40.0 23 33.42472862059836 38.0 31.0 39.0 20.0 40.0 24 32.683823140058244 38.0 30.0 39.0 17.0 40.0 25 33.180725443473655 38.0 31.0 39.0 19.0 40.0 26 33.429494307651574 38.0 33.0 39.0 19.0 40.0 27 33.75218427323272 38.0 33.0 39.0 21.0 40.0 28 33.81413820492455 38.0 33.0 39.0 22.0 40.0 29 33.22509928514694 38.0 32.0 39.0 19.0 40.0 30 33.08917129997352 38.0 32.0 39.0 18.0 40.0 31 33.86036536934075 38.0 33.0 39.0 22.0 40.0 32 33.7729414879534 38.0 33.0 39.0 22.0 40.0 33 33.52941487953402 38.0 33.0 39.0 20.0 40.0 34 33.32374900714853 38.0 33.0 39.0 19.0 40.0 35 33.489541964522104 38.0 33.0 39.0 20.0 40.0 36 32.51088165210484 36.0 31.0 39.0 17.0 40.0 37 32.22531109346042 36.0 31.0 39.0 14.0 40.0 38 31.519036272173683 36.0 30.0 38.0 8.0 40.0 39 30.372888535875035 35.0 28.0 38.0 4.0 40.0 40 28.100503044744507 34.0 20.0 38.0 4.0 40.0 41 25.915647339158063 31.0 14.0 38.0 4.0 39.0 42 26.811225840614245 31.0 19.0 37.0 4.0 39.0 43 27.230553349218958 32.0 20.0 38.0 4.0 39.0 44 26.393380990203866 31.0 17.0 38.0 4.0 39.0 45 27.291554143500132 32.0 21.0 38.0 4.0 39.0 46 25.329573735769127 30.0 16.0 36.0 4.0 39.0 47 24.433730473921102 29.0 11.0 36.0 4.0 39.0 48 24.920571882446385 29.0 15.0 36.0 4.0 39.0 49 23.888694731268203 28.0 9.0 35.0 4.0 39.0 50 24.73057982525814 29.0 13.0 36.0 4.0 39.0 51 24.92766746094784 30.0 8.0 36.0 4.0 39.0 52 24.832406671961873 30.0 4.0 36.0 4.0 39.0 53 24.721419115700293 30.0 4.0 36.0 4.0 39.0 54 24.380301826846704 30.0 4.0 36.0 4.0 39.0 55 24.27741593857559 30.0 4.0 36.0 4.0 39.0 56 23.638178448504103 30.0 4.0 36.0 4.0 39.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1 1 0.5601131718429073 1 2 1.1689924868607235 1 3 1.7454556273626025 1 4 1.3420339032172208 1 5 1.3768319975021583 1 6 2.7528873776546234 1 7 2.0392219188714478 1 8 2.105944124414748 1 9 2.091342703258448 1 10 2.8898143137631607 1 11 -0.1120272454134863 1 12 -5.660218481250951 1 13 -18.74942284045247 1 14 -4.555177909717919 1 15 0.9387814754002939 1 16 -1.252811332720384 1 17 -4.914542031399698 1 18 -2.5183572169388846 1 19 -0.008324519331139868 1 20 -0.08987091420581805 1 21 6.647224034838093 1 22 7.4714364494529235 1 23 6.264137209354363 1 24 7.034870854813722 1 25 6.375394078026673 1 26 7.072195781216621 1 27 6.604355102493475 1 28 6.545714779245465 1 29 6.916968388714746 1 30 7.539921455860082 1 31 6.0263544966114715 1 32 5.451777941028897 1 33 5.57828702688203 1 34 6.508275087721419 1 35 6.667628697700049 1 36 4.829345601808452 1 37 5.022128723746107 1 38 6.176515698399339 1 39 5.224404015252269 1 40 -10.49293354698996 1 41 0.43054003150588827 1 42 -1.3783726562908285 1 43 -9.088579047301621 1 44 -15.545125005619232 1 45 -2.710954321034329 1 46 -4.530800512508534 1 47 -9.518832315042594 1 48 -3.468074034237805 1 49 -13.23192481266451 1 50 -2.748219618324537 1 51 3.363633501124614 1 52 3.7424933657533437 1 53 2.868963141648596 1 54 3.6732448109630695 1 55 2.8886022831081632 1 56 7.247966780718393 2 1 0.9843555960853294 2 2 1.1008106686789034 2 3 1.9857153676223405 2 4 1.5325100936934106 2 5 1.652806023476181 2 6 2.882757507524751 2 7 2.2296981093476376 2 8 2.1200133884840184 2 9 2.667100279016026 2 10 3.4731476470964964 2 11 4.421522538136294 2 12 2.1882663672339007 2 13 2.7722221811925465 2 14 5.747852393312385 2 15 6.592461129079947 2 16 6.1227297928207385 2 17 7.906886540028875 2 18 6.260863562281891 2 19 7.581502320495698 2 20 6.3332892589543555 2 21 7.679691567305628 2 22 8.594813072829545 2 23 7.816085261302415 2 24 7.561927131870004 2 25 7.508510961143557 2 26 6.649035608056444 2 27 6.192017440155812 2 28 5.9656281991588855 2 29 6.776275748022105 2 30 6.9987959147345435 2 31 4.874839345096323 2 32 6.251561490812453 2 33 5.405126853721853 2 34 6.128404957851291 2 35 6.1643819444533 2 36 7.664843437306292 2 37 8.380353831971213 2 38 8.667857689741336 2 39 8.701676742524995 2 40 7.331741777685362 2 41 12.168635269601126 2 42 10.800198772280599 2 43 10.420078961356385 2 44 -2.246423706917934 2 45 11.137530527450522 2 46 9.07093108922307 2 47 7.830734784524505 2 48 10.768938952775184 2 49 1.6620145812748852 2 50 10.575373455268537 2 51 13.408005795496905 2 52 12.570415443675426 2 53 12.68606270874816 2 54 13.648353036071292 2 55 12.18405682856271 2 56 13.900564183315797 3 1 1.0288631718429073 3 2 1.4814924868607235 3 3 2.031169913076887 3 4 1.605426760360075 3 5 1.7295105689307277 3 6 3.0162802347974775 3 7 2.396364776014302 3 8 1.9363012672718938 3 9 2.247592703258448 3 10 3.0460643137631607 3 11 4.990651326015083 3 12 6.246031518749049 3 13 6.330934302404671 3 14 5.976072090282081 3 15 1.6530671896860092 3 16 7.425760095851043 3 17 8.875636540028875 3 18 9.499499925918258 3 19 8.134532623526002 3 20 7.615486228651324 3 21 7.767759749123808 3 22 8.123222163738639 3 23 7.656994352211509 3 24 7.838442283385152 3 25 7.335215506598104 3 26 7.523088638359475 3 27 6.251676531064906 3 28 6.554643350674034 3 29 7.832146960143316 3 30 7.218492884431512 3 31 5.5888544966114715 3 32 5.317849369600328 3 33 6.0604298840248845 3 34 5.432382230578565 3 35 5.37745012627148 3 36 6.168631316094167 3 37 6.798914438031822 3 38 7.9131228412564845 3 39 7.849404015252269 3 40 10.899923595867183 3 41 13.376968602934461 3 42 10.956448772280599 3 43 11.799813809841236 3 44 11.08880356580934 3 45 11.664045678965671 3 46 13.696878058920039 3 47 13.713310542100263 3 48 14.02746168004791 3 49 14.23682518733549 3 50 14.506244667389748 3 51 12.600240643981756 3 52 12.644279080039059 3 53 12.449320284505738 3 54 13.365209096677354 3 55 10.076102283108163 3 56 11.01135963786125 4 1 1.2267798385095716 4 2 1.6189924868607264 4 3 2.16450324641022 4 4 1.8991767603600778 4 5 1.864927235597392 4 6 3.276696901464142 4 7 2.563031442680966 4 8 2.432134600605224 4 9 2.658009369925118 4 10 3.373147647096495 4 11 4.8094013260150845 4 12 6.2064481854157165 4 13 6.960100969071341 4 14 6.244822090282078 4 15 6.619733856352678 4 16 8.259093429184379 4 17 9.940219873362206 4 18 10.25783325925159 4 19 8.75119929019267 4 20 7.969652895317989 4 21 8.028176415790472 4 22 8.752388830405309 4 23 7.973661018878179 4 24 8.613442283385151 4 25 8.087298839931435 4 26 7.379338638359478 4 27 6.616259864398241 4 28 7.050476684007364 4 29 6.988396960143316 4 30 7.535159551098182 4 31 6.159687829944804 4 32 5.997016036266995 4 33 6.568763217358217 4 34 6.401132230578565 4 35 6.712866792938147 4 36 8.043631316094167 4 37 7.653081104698487 4 38 8.952706174589817 4 39 8.874404015252267 4 40 3.4165902625338482 4 41 14.335301936267797 4 42 13.433532105613931 4 43 12.801897143174568 4 44 13.292970232476005 4 45 12.355712345632341 4 46 14.340628058920036 4 47 15.254977208766928 4 48 15.029545013381242 4 49 14.801408520668822 4 50 15.787494667389748 4 51 14.956490643981759 4 52 14.173445746705728 4 53 14.661820284505737 4 54 14.642292430010684 4 55 15.638602283108163 4 56 14.78844297119458 5 1 0.2524208641506007 5 2 1.111300179168417 5 3 1.7619391438461207 5 4 1.1222536834369947 5 5 1.6213374920076475 5 6 2.7254148501820907 5 7 2.3194416990912217 5 8 2.2680320365026603 5 9 1.8990350109507546 5 10 3.0628912368400805 5 11 5.09401671063047 5 12 6.224396903364436 5 13 7.047280456250824 5 14 6.214052859512847 5 15 7.153067189686009 5 16 7.887298557389503 5 17 9.406886540028875 5 18 9.624499925918258 5 19 8.384532623526002 5 20 7.8799093055744045 5 21 7.784586672200728 5 22 6.993414471430945 5 23 7.676225121442275 5 24 8.328826898769766 5 25 7.40011935275195 5 26 5.9485694075902416 5 27 6.013695761834139 5 28 6.7325279660586475 5 29 5.8345508062971625 5 30 7.622339038277666 5 31 4.641739111996085 5 32 5.8611186003695614 5 33 6.089276037871038 5 34 6.439593769040105 5 35 6.34620012627148 5 36 7.6590159314787805 5 37 7.6017990534164355 5 38 5.473218995102638 5 39 7.666711707559962 5 40 9.880692826636416 5 41 12.809660910626768 5 42 11.184814156895985 5 43 11.92240996368739 5 44 12.60322664273242 5 45 10.673661063581058 5 46 10.271397289689272 5 47 12.049849003638723 5 48 11.03467321850945 5 49 13.498844418104724 5 50 12.325956205851288 5 51 11.41033679782791 5 52 9.822163695423672 5 53 9.261820284505738 5 54 10.747420635138894 5 55 11.215525360031243 5 56 10.508955791707404 6 1 0.7386846004143379 6 2 0.9189924868607235 6 3 1.566884198791172 6 4 1.3777481889315055 6 5 1.3411177117878736 6 6 1.7171730919403316 6 7 1.003507633157156 6 8 1.1059441244147479 6 9 0.841342703258448 6 10 1.8898143137631607 6 11 4.245115611729368 6 12 5.01835294732048 6 13 6.3577200166903864 6 14 5.337679233139227 6 15 6.58163861825744 6 16 6.425760095851043 6 17 8.728315111457444 6 18 9.231642783061112 6 19 7.027389766383148 6 20 6.44584337150847 6 21 5.861509749123808 6 22 7.257150735167208 6 23 7.156994352211509 6 24 7.034870854813722 6 25 7.161108363740958 6 26 5.536481495502329 6 27 4.997212245350621 6 28 4.652857636388319 6 29 6.09553981728617 6 30 5.218492884431512 6 31 4.0263544966114715 6 32 4.344635083886043 6 33 3.864001312596315 6 34 3.722560802007134 6 35 4.560485840557195 6 36 5.150774173237021 6 37 4.700700152317538 6 38 7.2836585555422 6 39 6.688689729537984 6 40 1.3642093101528978 6 41 10.716254317220177 6 42 10.907341629423453 6 43 9.089992381269806 6 44 9.240589280095055 6 45 9.289045678965671 6 46 10.933485201777184 6 47 12.23116768495741 6 48 10.960497394333625 6 49 9.803789473049775 6 50 10.537494667389748 6 51 9.649347786838902 6 52 9.528207651467628 6 53 8.297534570220023 6 54 8.994673382391639 6 55 8.638602283108163 6 56 8.85510963786125 7 1 0.5971502088799454 7 2 0.7708443387125783 7 3 1.5311699130768872 7 4 1.1251026862859987 7 5 0.9760383467085063 7 6 2.0544746792419204 7 7 1.0445129241624471 7 8 1.5173197857904128 7 9 0.8320834439991884 7 10 2.732406906355756 7 11 5.283475400089159 7 12 5.710151889119423 7 13 6.523063932034297 7 14 5.185562831022821 7 15 6.13454867116749 7 16 7.129463799554745 7 17 8.79577542891776 7 18 8.476351777770113 7 19 8.328977067970445 7 20 5.228912154577248 7 21 5.935583823197884 7 22 7.278314756331234 7 23 5.536623981841135 7 24 6.7504793204221905 7 25 6.9798914325240276 7 26 5.457116416137254 7 27 3.1718154199537913 7 28 4.154180387711072 7 29 3.173582145328499 7 30 4.26478918072781 7 31 2.5819100521670286 7 32 3.3896086288595875 7 33 3.3983928469878464 7 34 3.8455766750230076 7 35 3.0128667929381443 7 36 4.24733501979787 7 37 5.5790070306244175 7 38 4.96752098940463 7 39 6.307737348585604 7 40 6.6314050773486635 7 41 11.613079714045575 7 42 10.429828401910225 7 43 10.527823069100496 7 44 11.359636899142675 7 45 10.214971604891595 7 46 9.966553984845962 7 47 11.366088319878042 7 48 11.273989457825689 7 49 10.194001113261418 7 50 11.343050222945305 7 51 11.219453606944718 7 52 11.42900130226128 7 53 11.298857321542776 7 54 11.33858872630698 7 55 11.749713394219278 7 56 8.169924452676064 8 1 0.32011317184290533 8 2 1.1589924868607255 8 3 1.6511699130768847 8 4 1.439176760360077 8 5 1.3582605689307243 8 6 2.4100302347974747 8 7 1.4963647760143033 8 8 1.558801267271896 8 9 1.9713427032584505 8 10 2.5798143137631584 8 11 4.5694013260150825 8 12 6.059781518749048 8 13 5.773434302404674 8 14 5.964822090282084 8 15 7.29306718968601 8 16 7.865760095851041 8 17 8.106886540028878 8 18 9.664499925918257 8 19 7.684532623526 8 20 7.582986228651325 8 21 6.901509749123807 8 22 7.725722163738638 8 23 6.86699435221151 8 24 7.15344228338515 8 25 7.133965506598102 8 26 6.079338638359474 8 27 4.042926531064904 8 28 4.557143350674032 8 29 6.068396960143314 8 30 5.528492884431515 8 31 5.946354496611473 8 32 4.69034936960033 8 33 5.315429884024887 8 34 5.8411322305785625 8 35 4.866200126271483 8 36 6.023631316094164 8 37 7.586414438031824 8 38 6.659372841256488 8 39 8.294404015252269 8 40 2.8699235958671814 8 41 13.021968602934464 8 42 11.960198772280595 8 43 9.748563809841233 8 44 11.706303565809339 8 45 11.129045678965674 8 46 12.120628058920037 8 47 12.028310542100261 8 48 11.376211680047913 8 49 13.30807518733549 8 50 13.587494667389745 8 51 12.656490643981755 8 52 10.606779080039058 8 53 10.54182028450574 8 54 10.908959096677357 8 55 12.038602283108162 8 56 12.71510963786125 9 1 0.040113171842904194 9 2 0.8789924868607244 9 3 1.211169913076887 9 4 0.5191767603600752 9 5 0.9182605689307266 9 6 2.49003023479748 9 7 1.416364776014305 9 8 1.518801267271897 9 9 1.6513427032584502 9 10 2.219814313763159 9 11 3.969401326015081 9 12 5.019781518749049 9 13 5.853434302404672 9 14 4.8848220902820785 9 15 7.013067189686009 9 16 7.145760095851042 9 17 7.666886540028873 9 18 10.024499925918256 9 19 7.444532623526005 9 20 6.262986228651325 9 21 7.341509749123805 9 22 7.085722163738637 9 23 6.826994352211511 9 24 8.073442283385152 9 25 6.773965506598103 9 26 5.2793386383594765 9 27 6.122926531064909 9 28 5.917143350674031 9 29 6.588396960143317 9 30 6.528492884431515 9 31 2.706354496611471 9 32 3.8103493696003277 9 33 4.555429884024882 9 34 3.1611322305785627 9 35 5.306200126271481 9 36 6.1036313160941695 9 37 4.506414438031825 9 38 5.179372841256484 9 39 6.614404015252269 9 40 1.509923595867182 9 41 9.741968602934463 9 42 9.3601987722806 9 43 8.428563809841233 9 44 7.866303565809339 9 45 8.209045678965673 9 46 10.00062805892004 9 47 10.828310542100265 9 48 11.17621168004791 9 49 11.62807518733549 9 50 10.66749466738975 9 51 9.256490643981756 9 52 10.80677908003906 9 53 9.621820284505738 9 54 9.228959096677354 9 55 8.998602283108163 9 56 6.755109637861249 10 1 0.8328404445701807 10 2 0.6917197595879969 10 3 1.712988094895067 10 4 1.5628131239964418 10 5 1.652806023476181 10 6 2.7463938711611107 10 7 1.8963647760143019 10 8 1.953346721817347 10 9 1.0458881578039012 10 10 2.548905222854067 10 11 5.209401326015083 10 12 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8.039045678965671 114 46 3.5406280589200385 114 47 5.463310542100263 114 48 3.93371168004791 114 49 -7.856924812664509 114 50 6.162494667389748 114 51 4.943990643981756 114 52 4.456779080039059 114 53 4.886820284505738 114 54 4.521459096677354 114 55 4.388602283108163 114 56 5.355109637861251 115 1 0.20717199537232034 115 2 0.8013454280371946 115 3 1.4135228542533582 115 4 1.081529701536546 115 5 1.0806135101071987 115 6 2.4923831759739485 115 7 1.7198941877790048 115 8 1.6458600908013068 115 9 1.914872115023151 115 10 2.0809907843513926 115 11 3.091754267191554 115 12 3.0456638716902233 115 13 1.0699048906399682 115 14 -1.6728249685414482 115 15 -1.2292857514904618 115 16 3.425760095851043 115 17 5.083357128264172 115 18 5.036264631800613 115 19 5.1786502705848285 115 20 4.479456816886621 115 21 7.273274455006163 115 22 8.273957457856284 115 23 6.671700234564451 115 24 7.713442283385152 115 25 7.112789036009872 115 26 5.738162167771243 115 27 5.929985354594319 115 28 5.470084527144621 115 29 6.811926371908019 115 30 7.262610531490338 115 31 5.3204721436702975 115 32 5.777408193129741 115 33 4.9648416487307685 115 34 3.8128969364609198 115 35 4.757964832153835 115 36 5.837748963152993 115 37 5.574649732149467 115 38 6.2311375471388395 115 39 5.915580485840501 115 40 9.796982419396596 115 41 6.913733308816813 115 42 6.1531399487511855 115 43 4.562681456900059 115 44 -6.267814081249483 115 45 4.9949280319068485 115 46 -2.1946660587270195 115 47 -8.411689457899737 115 48 -1.5626118493638543 115 49 -7.084865989135098 115 50 -1.7419170973161364 115 51 1.5506082910405787 115 52 1.559720256509646 115 53 1.5559379315645607 115 54 1.8266061555008832 115 55 1.0503669889905147 115 56 4.060991990802428 116 1 0.6905479544516027 116 2 1.0929055303389816 116 3 1.7050829565551453 116 4 1.373089803838333 116 5 1.3721736124089858 116 6 2.4361171913192194 116 7 1.4181039064490832 116 8 1.6944534411849403 116 9 2.091342703258448 116 10 2.9224230094153327 116 11 3.86157523905856 116 12 3.5136945622273075 116 13 5.502129954578585 116 14 5.053517742455995 116 15 -5.868671940748772 116 16 3.3822818349814803 116 17 4.254712626985398 116 18 5.233195578092172 116 19 4.6671413191781745 116 20 4.302986228651324 116 21 6.296292357819457 116 22 7.424852598521248 116 23 6.298298700037595 116 24 6.66996402251559 116 25 6.619182897902455 116 26 6.9836864644464285 116 27 5.717709139760554 116 28 4.291056394152292 116 29 6.59709261231723 116 30 5.88153636269238 116 31 4.20026754008973 116 32 5.086871108730765 116 33 5.261516840546626 116 34 5.662001795795955 116 35 4.650547952358433 116 36 7.239283490007214 116 37 7.116849220640518 116 38 7.732416319517352 116 39 9.409186623947917 116 40 9.541227943693269 116 41 9.067185994238812 116 42 9.452372685324075 116 43 1.5294333750586269 116 44 1.591520957113687 116 45 7.158610896356976 116 46 2.6493237110939525 116 47 -5.0638633709432135 116 48 3.2353421148305195 116 49 -3.7319248126645093 116 50 2.4396685804332243 116 51 5.256490643981756 116 52 5.598083427865145 116 53 6.827037675810086 116 54 6.143741705373007 116 55 6.073384891803816 116 56 11.920327029165602 117 1 -0.14576918109826664 117 2 0.21311013391954958 117 3 0.8252875601357132 117 4 0.49329440741890096 117 5 0.4923782159895538 117 6 1.7865008230327746 117 7 1.190482423073128 117 8 1.0576247966836618 117 9 1.385460350317274 117 10 2.2574613725866897 117 11 1.150577796603315 117 12 -6.601394951839186 117 13 -8.930095109360035 117 14 -0.6728249685414482 117 15 -3.46457986913752 117 16 -8.809534021796015 117 17 1.906886540028875 117 18 -0.8460883093758582 117 19 2.0021796823495315 117 20 0.9500450521807373 117 21 5.096803866770866 117 22 6.509251575503342 117 23 5.024641411035038 117 24 6.360501106914565 117 25 5.171612565421633 117 26 6.385220991300649 117 27 5.341750060476674 117 28 5.528908056556389 117 29 6.341338136613903 117 30 6.439081119725628 117 31 3.9675309671997034 117 32 4.012702310776799 117 33 5.7883710604954715 117 34 4.930543995284449 117 35 5.169729538036183 117 36 6.661278374917696 117 37 6.104061496855351 117 38 6.9958434294917815 117 39 8.092051074075798 117 40 8.561688301749538 117 41 8.09020389705211 117 42 7.506081125221776 117 43 -3.496142072511706 117 44 -0.5031081988965447 117 45 6.759633914259787 117 46 -1.8417248822564325 117 47 3.0000752479826147 117 48 1.0256234447537942 117 49 1.3268987167472552 117 50 4.434553490919161 117 51 8.197667114569992 117 52 8.61854378592141 117 53 7.202996755093974 117 54 8.003076743736177 117 55 7.579778753696399 117 56 9.766874343743602 118 1 -0.4398868281570927 118 2 0.10649248686072355 118 3 0.7186699130768872 118 4 0.3241767603600749 118 5 0.19826056893072774 118 6 0.9850302347974775 118 7 1.0213647760143019 118 8 0.9363012672718938 118 9 0.966342703258448 118 10 1.7023143137631607 118 11 -1.165598673984917 118 12 -11.22271848125095 118 13 -9.544065697595329 118 14 -10.36767790971792 118 15 -10.90943281031399 118 16 -10.574239904148957 118 17 -1.593113459971125 118 18 -6.438000074081742 118 19 -0.9904673764739975 118 20 -1.9470137713486757 118 21 3.736509749123808 118 22 4.810722163738639 118 23 3.594494352211509 118 24 4.838442283385152 118 25 4.366465506598104 118 26 3.491838638359475 118 27 3.4079265310649056 118 28 2.4296433506740343 118 29 2.925896960143316 118 30 3.1559928844315124 118 31 2.5263544966114715 118 32 3.692849369600328 118 33 2.9354298840248845 118 34 1.7136322305785647 118 35 2.28370012627148 118 36 4.418631316094167 118 37 4.736414438031822 118 38 5.7568728412564845 118 39 6.599404015252269 118 40 8.524923595867183 118 41 8.126968602934461 118 42 6.6751987722805985 118 43 -0.04393619015876382 118 44 2.7138035658093393 118 45 7.164045678965671 118 46 2.8531280589200385 118 47 -2.9116894578997368 118 48 -0.5037883199520898 118 49 2.7680751873354907 118 50 2.4124946673897476 118 51 6.318990643981756 118 52 5.019279080039059 118 53 4.324320284505738 118 54 5.583959096677354 118 55 6.263602283108163 118 56 4.980109637861251 119 1 -0.22936051236762012 119 2 0.28741353949230586 119 3 0.636433070971627 119 4 0.5675978129916572 119 5 0.5666816215623101 119 6 1.6100302347974775 119 7 0.7384700391721992 119 8 1.1566960041139964 119 9 1.3018690190479205 119 10 2.508235366394743 119 11 1.6304539575940282 119 12 -3.765481639145687 119 13 -0.6328814870690138 119 14 -3.6604410676126555 119 15 -10.189038073471885 119 16 -3.4689767462542207 119 17 1.1174128558183476 119 18 -5.375500074081742 119 19 -1.1154673764739975 119 20 -0.17069798187499075 119 21 5.019404485965911 119 22 5.422564269001796 119 23 4.906994352211509 119 24 5.50291596759568 119 25 5.106597085545474 119 26 4.20565442783316 119 27 4.3881896889596455 119 28 4.222406508568774 119 29 5.830502223301213 119 30 5.60007183179993 119 31 4.184249233453574 119 32 4.8671914748634855 119 33 5.119640410340672 119 34 4.82218486215751 119 35 4.504094863113583 119 36 6.280473421357325 119 37 6.986414438031822 119 38 7.450951788624902 119 39 6.921772436304899 119 40 10.676239385340867 119 41 7.238810708197619 119 42 6.642304035438492 119 43 -2.731436190158764 119 44 1.7631456710724969 119 45 4.499571994755144 119 46 0.1985227957621447 119 47 -1.1485315631628943 119 48 -6.187998846267881 119 49 1.373338345230227 119 50 0.9980209831792202 119 51 5.203859065034386 119 52 5.154147501091689 119 53 4.84076765292679 119 54 5.972116991414197 119 55 5.901760177845006 119 56 7.355109637861251 120 1 -0.5575338869806217 120 2 -0.31630163078633444 120 3 -0.29235949868781574 120 4 0.49329440741890096 120 5 0.19826056893072774 120 6 0.49238317597394854 120 7 0.4257765407201859 120 8 1.1752718555071908 120 9 1.150166232670216 120 10 1.4339319608219867 120 11 -0.7317751445731489 120 12 -4.77786554007448 120 13 -4.753624521124742 120 14 -3.4963543803061548 120 15 -0.7586975161963423 120 16 -6.044828139443073 120 17 1.0833571282641685 120 18 -3.6107941917288002 120 19 -0.5860556117681135 120 20 -1.5205431831133822 120 21 3.743862690300279 120 22 6.332780987268052 120 23 5.024641411035038 120 24 3.1840305186792683 120 25 3.7010243301275167 120 26 3.208750403065359 120 27 3.8711618251825506 120 28 3.6465551153799183 120 29 4.223691077790374 120 30 4.909669355019744 120 31 2.9087074377879425 120 32 2.718584663717973 120 33 3.3177828252013555 120 34 3.1070145835197387 120 35 3.34620012627148 120 36 3.36716072785887 120 37 3.8687673792082933 120 38 4.2899610765506 120 39 5.85675695642874 120 40 6.8558059488083565 120 41 -8.027443161771421 120 42 -7.376271815954695 120 43 -6.672612660746999 120 44 -16.679578787131838 120 45 -5.828601379857858 120 46 -8.135842529315255 120 47 -6.7646306343703255 120 48 -6.915553025834443 120 49 -15.673101283252745 120 50 -3.7419170973161364 120 51 1.6094318204523432 120 52 1.0303084918037655 120 53 1.6735849903880897 120 54 0.6501355672655897 120 55 1.9915434595787502 120 56 4.649227284920073 >>END_MODULE >>Per sequence quality scores pass #Quality Count 4 23.0 5 13.0 6 95.0 7 44.0 8 171.0 9 287.0 10 75.0 11 74.0 12 57.0 13 29.0 14 42.0 15 57.0 16 49.0 17 72.0 18 101.0 19 148.0 20 181.0 21 255.0 22 255.0 23 440.0 24 505.0 25 594.0 26 611.0 27 652.0 28 668.0 29 653.0 30 739.0 31 882.0 32 929.0 33 1108.0 34 1254.0 35 1363.0 36 1432.0 37 1569.0 38 1675.0 39 1783.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 0.0 0.0 0.0 100.0 2 0.0 0.0 0.0 100.0 3 0.0 0.0 100.0 0.0 4 0.0 0.0 100.0 0.0 5 0.0 0.0 100.0 0.0 6 0.0 0.0 0.0 100.0 7 99.33809902038655 0.4553878739740535 0.14826581943341274 0.05824728620598359 8 99.31691818903892 0.41832141911570025 0.18003706645485837 0.08472332539052159 9 97.32592004236166 0.5507016150383902 1.5885623510722795 0.5348159915276675 10 37.88721207307387 28.334657135292563 23.913158591474716 9.864972200158856 11 26.110669843791367 22.234577707175006 19.973523960815463 31.68122848821816 12 25.416997617156472 30.27799841143765 19.173947577442416 25.131056393963462 13 19.438707969287794 13.269790839290444 22.53640455387874 44.755096637543026 14 19.86761980407731 38.231400582472865 19.888800635424943 22.012178978024888 15 38.68632565071592 19.215414914568584 20.08835897162932 22.009900463086176 16 24.72713786160856 36.45226237151637 21.166684327646497 17.65391543922857 17 23.29855410200731 18.696043641756262 38.63142842010487 19.37397383613156 18 20.550701615038392 18.633836378077838 21.71035213132115 39.10510987556262 19 18.787750344389107 20.25537776835859 21.744198368125463 39.21267351912684 20 20.253500212134067 20.64594823928723 38.332626219770894 20.767925328807806 21 19.158061953931693 18.23140058247286 24.36325125761186 38.247286205983585 22 16.865236960550703 17.002912364310298 43.22478157267673 22.90706910246227 23 17.537728355837967 20.646015356102726 39.465184008472335 22.351072279586973 24 15.870578267316246 21.949798771446726 24.676975217115018 37.50264774412201 25 20.633407478021397 22.45524838470501 36.48977862514564 20.421565512127955 26 39.94383808413691 24.663558334216383 17.786372787962275 17.606230793684432 27 20.584026710477502 38.25851926440193 18.416450262335047 22.74100376278552 28 29.531374106433677 30.548054011119934 21.05904156738152 18.86153031506487 29 31.332908840072164 29.672078955746578 19.250769394035867 19.744242810145387 30 28.079470198675498 21.160264900662252 31.43841059602649 19.32185430463576 31 32.77195212371571 29.32952017794725 21.226564982523037 16.671962715814004 32 29.770102765123426 31.465197584489886 20.66956245364975 18.095137196736943 33 31.375874496502014 27.517489930040277 22.026711893152427 19.079923680305278 34 20.519756032882526 22.736674622116148 38.84911164147441 17.894457703526918 35 19.778296382730456 22.790919698737667 39.853611965630634 17.577171952901242 36 28.059764755748652 21.987919889795485 31.720885874748333 18.231429479707533 37 28.378235044548155 20.752015273652948 33.13534153585066 17.734408145948237 38 28.61995753715499 22.887473460721868 29.95753715498938 18.535031847133755 39 29.86048485491486 23.017346559864198 21.510795183279402 25.611373401941538 40 23.217052192959027 17.735990170415086 29.59559805545168 29.45135958117421 41 20.26896699528504 16.298756965280756 25.525075010715813 37.90720102871839 42 19.306536398874314 30.75983645728243 22.975627887219243 26.95799925662401 43 16.31070024439486 29.003294017638932 29.61428116034428 25.071724577621936 44 18.913598638008086 30.644818046392853 27.585656522664397 22.855926792934667 45 26.28616936554287 23.259888505441996 30.007963897000266 20.445978232014866 46 18.149920255183414 31.27591706539075 22.923976608187136 27.650186071238704 47 16.369443705083846 26.739419749800376 22.262443438914026 34.62869310620176 48 27.976474328405658 22.683198219678907 22.349388014624065 26.990939437291367 49 18.249415763756108 27.963671128107077 21.01657106437221 32.77034204376461 50 19.072383782636383 30.917002759499045 22.521757588622375 27.4888558692422 51 17.604847711688727 29.809174506989848 23.770796789454103 28.81518099186733 52 18.883641958932458 39.958614103040276 23.165490528996656 17.992253409030614 53 20.131662773412614 36.07984710129539 23.45508600552134 20.333404119770652 54 18.651446774621718 24.54473055481816 29.82744889832758 26.97637377223255 55 17.114485981308412 23.879566694987258 31.860662701784197 27.145284621920133 56 17.77164393014491 24.18387387865598 33.5527363448166 24.491745846382504 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 1.0 7 2.0 8 3.0 9 4.0 10 6.0 11 8.0 12 18.5 13 42.5 14 56.0 15 54.0 16 52.0 17 58.0 18 64.0 19 63.5 20 63.0 21 66.0 22 71.0 23 73.0 24 99.5 25 126.0 26 129.5 27 133.0 28 141.5 29 176.0 30 202.0 31 215.0 32 228.0 33 244.0 34 260.0 35 266.0 36 272.0 37 299.5 38 356.0 39 385.0 40 411.5 41 438.0 42 494.5 43 551.0 44 769.5 45 988.0 46 1431.0 47 1949.5 48 2025.0 49 1693.5 50 1362.0 51 1252.5 52 1143.0 53 1111.5 54 1131.0 55 1182.0 56 1078.5 57 975.0 58 984.5 59 994.0 60 971.0 61 948.0 62 808.5 63 591.0 64 513.0 65 489.0 66 465.0 67 387.0 68 309.0 69 289.5 70 270.0 71 227.0 72 175.0 73 166.0 74 111.0 75 56.0 76 44.5 77 33.0 78 38.5 79 62.5 80 81.0 81 54.5 82 28.0 83 16.0 84 4.0 85 2.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.5189303680169447 16 0.05824728620598359 17 0.021180831347630397 18 0.0 19 0.05824728620598359 20 0.15356102727032034 21 0.0 22 0.0 23 0.0 24 0.005295207836907599 25 0.015885623510722795 26 0.05824728620598359 27 0.08472332539052159 28 0.0 29 0.20651310563939632 30 0.052952078369075985 31 0.015885623510722795 32 0.037066454858353186 33 0.09001853322742918 34 0.15885623510722796 35 0.16415144294413556 36 0.05824728620598359 37 0.15356102727032034 38 0.23828435266084197 39 0.18003706645485837 40 0.8790045009266614 41 1.1702409319565792 42 0.2753508075191951 43 0.33359809372517873 44 0.4712734974847763 45 0.2647603918453799 46 0.3971405877680699 47 0.5295207836907598 48 0.06354249404289118 49 0.3018268467037331 50 0.21710352131321153 51 0.3812549642573471 52 0.20121789780248875 53 0.25946518400847235 54 0.2647603918453799 55 0.28064601535610273 56 0.24357956049774954 >>END_MODULE >>Sequence Length Distribution pass #Length Count 56 18885.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 72.89383108287 #Duplication Level Percentage of deduplicated Percentage of total 1 89.36510242626761 65.14164680963728 2 6.283597268632864 9.160709557850145 3 1.7071044602644196 3.733121525019857 4 0.8281272700857184 2.414614773629865 5 0.4431207322388493 1.6150383902568177 6 0.3922708121458667 1.715647339158062 7 0.20339968037193082 1.0378607360338894 8 0.09443556588696789 0.5507016150383902 9 0.06537846869097777 0.4289118347895155 >10 0.5738776696208049 7.942811755361398 >50 0.01452854859799506 0.6089489012443738 >100 0.02179282289699259 2.981202012178978 >500 0.00726427429899753 2.66878474980143 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source CCTTTCGGGTCACAGATCCTCTTCTGAGATGAGTTTTTGTTCAATCCGCCCAATCT 504 2.66878474980143 No Hit CCTTTCGGGTCACAGATCCTCTTCTGAAGGAGATTGGGCGGATTGAACAAAAACTC 335 1.7738946253640455 No Hit CCTTTCGGGTCACAGATCCTCTTCTGAAGGAGATTGGGCGCATTGAACAAAAACTC 121 0.6407201482658195 No Hit CCTTTCGGGTCACAGATCCTCTTCTGAGATGAGTTTTTGTCCAATCCGCCCAATCT 107 0.5665872385491131 No Hit CCTTTCGGGAACTGTGGTAATTCTAGAGCTAATACATGCCGACGGGCGCTGACCCC 62 0.3283028858882711 No Hit CCTTTCGGGGGGCGCTGACCCCCCTTCCCGGGGGGGGATGCGTGCATTTATCAGAT 53 0.28064601535610273 No Hit CCTTTCGGGGGTGAGCTCCCTCCCGGCTCCGGCCGGGGGTCGGGCGCCGGCGGCTT 50 0.2647603918453799 No Hit CCTTTCGGTCACAGATCCTCTTCTGAGATGAGTTTTTGTTCAATCCGCCCAATCTC 49 0.25946518400847235 No Hit CCTTTCGGGTCACAGATCCTCTTCTGAGATGAGTTTTTGTTCAATCCGCCCAAAAA 48 0.2541699761715647 No Hit CCTTTCGGGGTCTGTCCGTCCGTGGAGCCAGTCCTAGCCACATTATTGACAGTCTT 43 0.22769393698702675 No Hit CCTTTCGGGGAACGTCTGCCCTATCAACTTTCGATGGTAGTCGCCGTGCCTACCAT 43 0.22769393698702675 No Hit CCTTTCGGGGTGGCGGCGACGACCCATTCGAACGTCTGCCCTATCAACTTTCGATG 42 0.22239872915011916 No Hit CCTTTCGGGTCACAGATCCTCTTCTGAGATGAGTTTTTGCTCAATCCGCCCAATCT 40 0.21180831347630394 No Hit CCTTTCGGGTCACAGATCCTCTTCTGAAGGAGATTGGGCGGATTGACCAAAAACTC 39 0.20651310563939632 No Hit CCTTTCGGGGGTTCCTTTGGTCGCTCGCTCCTCTCCTACTTGGATAACTGTGGTAA 34 0.18003706645485837 No Hit CCTTTCGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAG 32 0.16944665078104318 No Hit CCTTTCGGGATCCAAGGAAGGCAGCAGGCGCGCAAATTACCCACTCCCGACCCGGG 30 0.15885623510722796 No Hit CCTTTCGGGTCACAGATCCTCTTCTGAAGGAGATTGGGCCGATTGAACAAAAACTC 30 0.15885623510722796 No Hit CCTTTCGGGGGAGAGGGAGCCTGAGAAACGGCTACCACATCCAAGGAAGGCAGCAG 29 0.15356102727032034 No Hit CCTTTCGGGTCACAGATCCTCTGAAGGAGATTGGGCGGATTGAACAAAAACTCATC 27 0.14297061159650515 No Hit CCTTTCGGGACCCACTCCCGACCCGGGGAGGTAGTGACGAAAAATAACAATACAGG 26 0.13767540375959755 No Hit CCTTTCGGGCTCCTACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCGAC 26 0.13767540375959755 No Hit CCTTTCGGGAGTTATGGTTCCTTTGGTCGCTCGCTCCTCTCCTACTTGGATAACTG 26 0.13767540375959755 No Hit CCTTTCGGGATGGTTCCTTTGGTCGCTCGCTCCTCTCCTACTTGGATAACTGTGGT 25 0.13238019592268996 No Hit CCTTTCGGGAGTGACGAAAAATAACAATACAGGACTCTTTCGAGGCCCTGTAATTG 25 0.13238019592268996 No Hit CCTTTCGGGGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCCATTCGAACGTC 24 0.12708498808578236 No Hit CCTTTCGGGACTTTGAAAAAATTAGAGTGTTCAAAGCAGGCCCGAGCCGCCTGGAT 21 0.11119936457505958 No Hit CCTTTCGGGGGGGAGGTAGTGACGAAAAATAACAATACAGGACTCTTTCGAGGCCC 20 0.10590415673815197 No Hit CCTTTCGGGGGTGACTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGC 19 0.10060894890124437 No Hit CCTTTCGGGTCCCCCCTCCTCTTCTGAGATGAGTTTTTGTTCCCTCCGCCCAATCT 19 0.10060894890124437 No Hit CCTTTCGGGAACTGTGGTAATTCTAGAGCTAATACATGCCCACGGGCGCTGACCCC 19 0.10060894890124437 No Hit CCTTTCGGGAGCCGCGGTAATTCCAGCTCCAATAGCGTATATTAAAGTTGCTGCAG 19 0.10060894890124437 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0 0.0 0.0 0.0 0.0 11 0.010590415673815197 0.0 0.0 0.0 0.0 12 0.015885623510722795 0.0 0.0 0.0 0.0 13 0.015885623510722795 0.0 0.0 0.0 0.0 14 0.08472332539052158 0.0 0.0 0.0 0.0 15 0.08472332539052158 0.0 0.0 0.0 0.0 16 0.08472332539052158 0.0 0.0 0.0 0.0 17 0.08472332539052158 0.0 0.0 0.0 0.0 18 0.08472332539052158 0.0 0.0 0.0 0.0 19 0.08472332539052158 0.0 0.0 0.0 0.0 20 0.10060894890124437 0.0 0.0 0.0 0.0 21 0.10060894890124437 0.0 0.0 0.0 0.0 22 0.10060894890124437 0.0 0.0 0.0 0.0 23 0.10060894890124437 0.0 0.0 0.0 0.0 24 0.10590415673815197 0.0 0.0 0.0 0.0 25 0.11119936457505956 0.0 0.0 0.0 0.0 26 0.12178978024887477 0.0 0.0 0.0 0.0 27 0.12708498808578236 0.0 0.0 0.0 0.0 28 0.13238019592268996 0.0 0.0 0.0 0.0 29 0.13238019592268996 0.0 0.0 0.0 0.0 30 0.13767540375959755 0.0 0.0 0.0 0.0 31 0.15356102727032037 0.0 0.0 0.0 0.0 32 0.15885623510722796 0.0 0.0 0.0 0.0 33 0.15885623510722796 0.0 0.0 0.0 0.0 34 0.16415144294413556 0.0 0.0 0.0 0.0 35 0.16415144294413556 0.0 0.0 0.0 0.0 36 0.17474185861795075 0.0 0.0 0.0 0.0 37 0.19062748212867356 0.0 0.0 0.0 0.0 38 0.19062748212867356 0.0 0.0 0.0 0.0 39 0.19062748212867356 0.0 0.0 0.0 0.0 40 0.19592268996558115 0.0 0.0 0.0 0.0 41 0.20651310563939634 0.0 0.0 0.0 0.0 42 0.20651310563939634 0.0 0.0 0.0 0.0 43 0.20651310563939634 0.0 0.0 0.0 0.0 44 0.24357956049774954 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TTGTTCC 30 9.134892E-7 50.60647 37 CATTGAA 35 4.4752596E-8 50.60647 41 GGCGCAT 30 9.134892E-7 50.60647 37 GTTCCCT 15 0.007915314 50.60647 39 GTTCCAT 15 0.007915314 50.60647 39 TGTTCCC 15 0.007915314 50.60647 38 TGTTCCA 15 0.007915314 50.60647 38 TTCTCAA 15 0.007915314 50.60647 38 GCGCATT 30 9.134892E-7 50.60647 38 TGGGCCG 15 0.007915314 50.60647 35 CGCATTG 35 4.4752596E-8 50.60647 39 GCCGATT 15 0.007915314 50.60647 38 GCATTGA 35 4.4752596E-8 50.60647 40 TCTCAAT 15 0.007915314 50.60647 39 CCGATTG 15 0.007915314 50.60647 39 CGATTGA 15 0.007915314 50.60647 40 TTGGGCC 15 0.008086023 50.335117 34 CCCGGTT 25 2.0253136E-5 49.933514 19 CATCCGC 25 2.0253136E-5 49.933514 43 GTATCAA 25 2.0253136E-5 49.933514 18 >>END_MODULE