FastQCFastQC Report
Fri 17 Jun 2016
SRR1526177_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526177_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3198
Sequences flagged as poor quality0
Sequence length40
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTTTCGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTT140.4377736085053158No Hit
CCTTTCGGGGATGTTGTCCTGGCTGTTGCCAAAAAAAAAA120.37523452157598497No Hit
CCTTTCGGGATGTTGTCCTGGCTGTTGCCAAAAAAAAAAA60.18761726078799248No Hit
CCTTTCGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTT60.18761726078799248No Hit
CCTTTCGGGCTCGTCTTAATCAGCAACATCTTTTGATACA50.15634771732332708No Hit
CCTTTCGGGGCCGAATAGCCTCTCCACCCCAGCGGCCGGA50.15634771732332708No Hit
CCTTTCGGGCTCTTCGGACTTGGGCTTGGGTTCCGGCGAC40.12507817385866166No Hit
CCTTTCGGGGATGTTGTCCTGGCTGTTGGGAAAAAAAAAA40.12507817385866166No Hit
CCTTTCGGGGCCGAATAGCCTCTCCACCCCAGCCGCCCGC40.12507817385866166No Hit
CCTTTCGGGGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT40.12507817385866166No Hit
CCTTTCGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA40.12507817385866166No Hit
CCTTTCGGGAAGCATGTATCAACGCAGAGTCGACTTTTTT40.12507817385866166No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGGAT251.6068063E-434.06
CGGGATG650.034.06
CTGTTGC301.0692087E-534.022
TTCGGGT251.6068063E-434.04
TTCGGGG1300.034.04
TTCGGGA1250.034.04
TTTCGGG2950.034.03
TCGGGGT357.0350325E-734.05
TCGGGGG357.0350325E-734.05
TCGGGGC200.002389612834.05
TCGGGGA404.5722118E-834.05
TCGGGAT750.034.05
TCGGGAA357.0350325E-734.05
CCTTTCG3200.033.468751
CTTTCGG3150.033.460322
GCTGTTG402.0011503E-629.7521
GGCTGTT501.1394946E-523.820
TGGCTGT501.1394946E-523.819
GGGATGT651.0592885E-723.5384627
TTGTCCT552.3874163E-521.63636213
Produced by FastQC (version 0.11.5)