##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1526177_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3198
Sequences flagged as poor quality	0
Sequence length	40
%GC	48
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.64759224515322	33.0	30.0	35.0	30.0	36.0
2	26.3036272670419	30.0	22.0	33.0	4.0	36.0
3	24.140087554721703	29.0	15.0	33.0	4.0	36.0
4	20.263289555972484	22.0	8.0	30.0	4.0	35.0
5	18.0140712945591	18.0	4.0	27.0	4.0	33.0
6	20.964665415884927	26.0	4.0	32.0	4.0	35.0
7	16.91838649155722	19.0	4.0	26.0	4.0	31.0
8	15.368667917448406	16.0	4.0	25.0	4.0	30.0
9	16.713883677298313	19.0	4.0	27.0	4.0	31.0
10	13.006879299562227	10.0	4.0	20.0	4.0	29.0
11	16.026266416510317	15.0	4.0	28.0	4.0	33.0
12	16.005003126954346	16.0	4.0	28.0	4.0	32.0
13	16.036585365853657	15.0	4.0	28.0	4.0	32.0
14	15.975297060662914	15.0	4.0	28.0	4.0	32.0
15	15.161038148843026	11.0	4.0	27.0	4.0	33.0
16	15.038148843026892	6.0	4.0	28.0	4.0	33.0
17	14.931519699812382	4.0	4.0	28.0	4.0	33.0
18	14.550343964978111	4.0	4.0	27.0	4.0	33.0
19	13.206691682301438	4.0	4.0	24.0	4.0	33.0
20	13.535334584115072	4.0	4.0	26.0	4.0	32.0
21	11.815509693558473	4.0	4.0	21.0	4.0	31.0
22	11.715447154471544	4.0	4.0	21.0	4.0	31.0
23	11.080675422138837	4.0	4.0	19.0	4.0	30.0
24	10.76360225140713	4.0	4.0	18.0	4.0	30.0
25	10.486241400875548	4.0	4.0	16.0	4.0	31.0
26	9.619449656035021	4.0	4.0	10.0	4.0	30.0
27	9.768918073796122	4.0	4.0	11.0	4.0	30.0
28	9.3692933083177	4.0	4.0	9.0	4.0	29.0
29	9.045028142589118	4.0	4.0	4.0	4.0	29.0
30	8.507191994996873	4.0	4.0	4.0	4.0	27.0
31	8.762038774233897	4.0	4.0	4.0	4.0	29.0
32	8.534396497811132	4.0	4.0	4.0	4.0	28.0
33	8.649155722326453	4.0	4.0	4.0	4.0	29.0
34	8.573483427141964	4.0	4.0	4.0	4.0	29.0
35	8.459349593495935	4.0	4.0	4.0	4.0	29.0
36	8.937148217636022	4.0	4.0	4.0	4.0	32.0
37	8.899312070043777	4.0	4.0	4.0	4.0	32.0
38	8.849280800500313	4.0	4.0	4.0	4.0	32.0
39	8.808630393996248	4.0	4.0	4.0	4.0	32.0
40	8.722013758599125	4.0	4.0	4.0	4.0	32.0
>>END_MODULE
>>Per tile sequence quality	fail
#Tile	Base	Mean
1	1	3.8735042735042775
1	2	4.822364672364678
1	3	3.8840455840455803
1	4	11.102564102564099
1	5	12.518518518518523
1	6	11.763247863247862
1	7	14.994871794871791
1	8	13.344729344729346
1	9	19.05299145299145
1	10	11.417236467236469
1	11	6.059544159544158
1	12	9.34301994301994
1	13	-2.612108262108265
1	14	3.860683760683761
1	15	5.073361823361822
1	16	-2.5552706552706557
1	17	-3.899715099715099
1	18	-7.626495726495726
1	19	-5.847578347578349
1	20	-1.2769230769230777
1	21	0.4519943019943007
1	22	-1.3883190883190863
1	23	-0.2668091168091191
1	24	0.09230769230769198
1	25	0.41652421652421623
1	26	-0.2934472934472936
1	27	-3.6388888888888893
1	28	1.194159544159545
1	29	-4.623076923076924
1	30	-4.702421652421654
1	31	-4.994586894586895
1	32	-4.8901709401709414
1	33	-4.956410256410258
1	34	-5.092307692307694
1	35	-4.913105413105415
1	36	-5.648005698005695
1	37	-5.566381766381767
1	38	-5.498860398860396
1	39	-5.382478632478632
1	40	-5.254700854700854
2	1	-14.876495726495722
2	2	-11.427635327635322
2	3	-19.11595441595442
2	4	-12.397435897435901
2	5	-7.981481481481477
2	6	-7.986752136752138
2	7	0.24487179487179134
2	8	-2.4052706552706535
2	9	-3.4470085470085507
2	10	-2.082763532763531
2	11	-7.190455840455842
2	12	-7.406980056980061
2	13	0.13789173789173503
2	14	-1.3893162393162388
2	15	-2.9266381766381784
2	16	-4.305270655270656
2	17	-2.399715099715099
2	18	3.873504273504274
2	19	-5.597578347578349
2	20	-0.5269230769230777
2	21	10.7019943019943
2	22	10.111680911680914
2	23	10.98319088319088
2	24	9.842307692307692
2	25	11.166524216524216
2	26	12.206552706552706
2	27	12.11111111111111
2	28	10.194159544159545
2	29	12.876923076923076
2	30	12.797578347578346
2	31	12.005413105413105
2	32	12.109829059829059
2	33	10.043589743589742
2	34	10.407692307692306
2	35	12.086894586894585
2	36	8.351994301994305
2	37	11.433618233618233
2	38	11.501139601139604
2	39	8.617521367521368
2	40	11.745299145299146
3	1	-18.709829059829055
3	2	-14.260968660968656
3	3	-21.11595441595442
3	4	-16.3974358974359
3	5	-13.481481481481477
3	6	-15.986752136752138
3	7	-11.255128205128209
3	8	-9.905270655270654
3	9	-10.44700854700855
3	10	-7.082763532763531
3	11	-10.190455840455842
3	12	-9.90698005698006
3	13	-10.362108262108265
3	14	-10.389316239316239
3	15	-9.926638176638178
3	16	-9.305270655270656
3	17	-9.399715099715099
3	18	-9.126495726495726
3	19	-8.097578347578349
3	20	-8.026923076923078
3	21	-6.298005698005699
3	22	-6.888319088319086
3	23	-6.016809116809119
3	24	-5.657692307692308
3	25	-5.833475783475784
3	26	-5.293447293447294
3	27	-5.388888888888889
3	28	-5.305840455840455
3	29	-4.623076923076924
3	30	-4.702421652421654
3	31	-4.994586894586895
3	32	-4.8901709401709414
3	33	-4.956410256410258
3	34	-5.092307692307694
3	35	-4.913105413105415
3	36	-5.648005698005695
3	37	-5.566381766381767
3	38	-5.498860398860396
3	39	-5.382478632478632
3	40	-5.254700854700854
4	1	-2.0431623931623903
4	2	-1.927635327635322
4	3	-16.782621082621084
4	4	-5.730769230769235
4	5	-1.4814814814814774
4	6	6.013247863247862
4	7	5.0782051282051235
4	8	13.761396011396014
4	9	-8.780341880341883
4	10	2.250569800569803
4	11	-2.5237891737891758
4	12	11.759686609686607
4	13	12.971225071225067
4	14	9.277350427350429
4	15	11.073361823361822
4	16	1.6947293447293443
4	17	-4.066381766381765
4	18	14.540170940170942
4	19	7.902421652421651
4	20	2.9730769230769223
4	21	7.701994301994301
4	22	7.7783475783475815
4	23	9.98319088319088
4	24	9.00897435897436
4	25	7.833190883190884
4	26	26.706552706552706
4	27	24.61111111111111
4	28	24.36082621082621
4	29	23.710256410256413
4	30	22.297578347578344
4	31	2.3387464387464387
4	32	4.4431623931623925
4	33	4.376923076923076
4	34	5.24102564102564
4	35	2.4202279202279193
4	36	4.351994301994305
4	37	2.1002849002848993
4	38	4.501139601139604
4	39	4.617521367521368
4	40	2.4119658119658123
5	1	-20.126495726495722
5	2	-21.177635327635322
5	3	-14.36595441595442
5	4	-16.3974358974359
5	5	-13.481481481481477
5	6	-15.986752136752138
5	7	-11.255128205128209
5	8	-9.905270655270654
5	9	-10.44700854700855
5	10	-7.082763532763531
5	11	-10.190455840455842
5	12	-9.90698005698006
5	13	-10.362108262108265
5	14	-10.389316239316239
5	15	-9.926638176638178
5	16	-9.305270655270656
5	17	-9.399715099715099
5	18	-9.126495726495726
5	19	-8.097578347578349
5	20	-8.026923076923078
5	21	-6.298005698005699
5	22	-6.888319088319086
5	23	-6.016809116809119
5	24	-5.657692307692308
5	25	-5.833475783475784
5	26	-5.293447293447294
5	27	-5.388888888888889
5	28	-5.305840455840455
5	29	-4.623076923076924
5	30	-4.702421652421654
5	31	-4.994586894586895
5	32	-4.8901709401709414
5	33	-4.956410256410258
5	34	-5.092307692307694
5	35	-4.913105413105415
5	36	-5.648005698005695
5	37	-5.566381766381767
5	38	-5.498860398860396
5	39	-5.382478632478632
5	40	-5.254700854700854
6	1	0.6235042735042775
6	2	5.472364672364677
6	3	3.4840455840455817
6	4	-7.797435897435902
6	5	0.3185185185185233
6	6	6.013247863247862
6	7	5.544871794871792
6	8	8.694729344729348
6	9	5.152991452991451
6	10	6.917236467236469
6	11	4.809544159544158
6	12	4.693019943019941
6	13	4.837891737891734
6	14	7.01068376068376
6	15	2.273361823361821
6	16	2.8947293447293436
6	17	9.600284900284901
6	18	8.873504273504274
6	19	10.902421652421651
6	20	10.37307692307692
6	21	9.1019943019943
6	22	4.911680911680914
6	23	14.18319088319088
6	24	15.342307692307692
6	25	14.966524216524217
6	26	13.306552706552708
6	27	17.81111111111111
6	28	9.094159544159544
6	29	10.376923076923076
6	30	8.297578347578346
6	31	3.8054131054131055
6	32	3.9098290598290593
6	33	7.44358974358974
6	34	6.707692307692307
6	35	1.2868945868945847
6	36	6.751994301994303
6	37	6.833618233618232
6	38	1.3011396011396048
6	39	5.217521367521368
6	40	3.745299145299146
7	1	3.223504273504279
7	2	6.472364672364677
7	3	7.284045584045579
7	4	6.2025641025641
7	5	6.918518518518521
7	6	9.813247863247863
7	7	8.94487179487179
7	8	9.894729344729347
7	9	7.7529914529914485
7	10	3.3172364672364694
7	11	6.40954415954416
7	12	9.69301994301994
7	13	8.037891737891734
7	14	7.8106837606837605
7	15	8.873361823361822
7	16	10.894729344729344
7	17	10.0002849002849
7	18	11.073504273504273
7	19	12.10242165242165
7	20	11.573076923076924
7	21	9.9019943019943
7	22	13.511680911680912
7	23	10.583190883190882
7	24	10.542307692307691
7	25	5.966524216524217
7	26	-5.293447293447294
7	27	-5.388888888888889
7	28	-5.305840455840455
7	29	-4.623076923076924
7	30	-4.702421652421654
7	31	-4.994586894586895
7	32	-4.8901709401709414
7	33	-4.956410256410258
7	34	-5.092307692307694
7	35	-4.913105413105415
7	36	-5.648005698005695
7	37	-5.566381766381767
7	38	-5.498860398860396
7	39	-5.382478632478632
7	40	-5.254700854700854
8	1	0.22350427350427893
8	2	3.8723646723646787
8	3	6.88404558404558
8	4	-15.397435897435901
8	5	-12.681481481481477
8	6	-12.186752136752137
8	7	-7.455128205128209
8	8	-6.105270655270654
8	9	-6.647008547008551
8	10	-6.082763532763531
8	11	-4.590455840455842
8	12	-6.106980056980061
8	13	-4.762108262108265
8	14	-10.389316239316239
8	15	-9.926638176638178
8	16	-9.305270655270656
8	17	-9.399715099715099
8	18	-9.126495726495726
8	19	-8.097578347578349
8	20	-8.026923076923078
8	21	-6.298005698005699
8	22	-6.888319088319086
8	23	-6.016809116809119
8	24	-5.657692307692308
8	25	-5.833475783475784
8	26	-5.293447293447294
8	27	-5.388888888888889
8	28	-5.305840455840455
8	29	-4.623076923076924
8	30	-4.702421652421654
8	31	-4.994586894586895
8	32	-4.8901709401709414
8	33	-4.956410256410258
8	34	-5.092307692307694
8	35	-4.913105413105415
8	36	-5.648005698005695
8	37	-5.566381766381767
8	38	-5.498860398860396
8	39	-5.382478632478632
8	40	-5.254700854700854
9	1	2.8235042735042803
9	2	2.4723646723646766
9	3	9.084045584045583
9	4	4.2025641025641
9	5	1.3185185185185233
9	6	12.613247863247864
9	7	1.3448717948717928
9	8	3.0947293447293465
9	9	-2.64700854700855
9	10	-0.48276353276353134
9	11	-6.190455840455842
9	12	-2.306980056980061
9	13	-2.7621082621082653
9	14	-5.9893162393162385
9	15	-2.9266381766381784
9	16	1.294729344729344
9	17	1.4002849002849018
9	18	1.4735042735042736
9	19	-1.297578347578348
9	20	2.1730769230769216
9	21	3.5019943019943014
9	22	-0.2883190883190867
9	23	1.9831908831908809
9	24	4.542307692307691
9	25	-0.23347578347578413
9	26	-5.293447293447294
9	27	-5.388888888888889
9	28	-5.305840455840455
9	29	-4.623076923076924
9	30	-4.702421652421654
9	31	-4.994586894586895
9	32	-4.8901709401709414
9	33	-4.956410256410258
9	34	-5.092307692307694
9	35	-4.913105413105415
9	36	-5.648005698005695
9	37	-5.566381766381767
9	38	-5.498860398860396
9	39	-5.382478632478632
9	40	-5.254700854700854
10	1	-5.776495726495721
10	2	-1.5276353276353234
10	3	-14.315954415954419
10	4	-6.997435897435901
10	5	-7.881481481481478
10	6	-12.986752136752138
10	7	-1.0551282051282094
10	8	-0.10527065527065282
10	9	-1.64700854700855
10	10	-3.082763532763531
10	11	6.009544159544157
10	12	5.89301994301994
10	13	2.0378917378917336
10	14	5.01068376068376
10	15	6.073361823361822
10	16	7.494729344729345
10	17	2.400284900284902
10	18	3.4735042735042754
10	19	4.702421652421652
10	20	3.1730769230769216
10	21	2.9019943019943
10	22	3.7116809116809133
10	23	4.783190883190882
10	24	4.942307692307692
10	25	4.966524216524217
10	26	-5.293447293447294
10	27	-5.388888888888889
10	28	-5.305840455840455
10	29	-4.623076923076924
10	30	-4.702421652421654
10	31	-4.994586894586895
10	32	-4.8901709401709414
10	33	-4.956410256410258
10	34	-5.092307692307694
10	35	-4.913105413105415
10	36	-5.648005698005695
10	37	-5.566381766381767
10	38	-5.498860398860396
10	39	-5.382478632478632
10	40	-5.254700854700854
11	1	-6.1264957264957225
11	2	-6.927635327635322
11	3	0.1340455840455803
11	4	-14.647435897435901
11	5	-10.481481481481477
11	6	-9.236752136752138
11	7	-11.255128205128209
11	8	-9.905270655270654
11	9	-10.44700854700855
11	10	-7.082763532763531
11	11	-10.190455840455842
11	12	-9.90698005698006
11	13	-10.362108262108265
11	14	-10.389316239316239
11	15	-9.926638176638178
11	16	-9.305270655270656
11	17	-9.399715099715099
11	18	-9.126495726495726
11	19	-8.097578347578349
11	20	-8.026923076923078
11	21	-6.298005698005699
11	22	-6.888319088319086
11	23	-6.016809116809119
11	24	-5.657692307692308
11	25	-5.833475783475784
11	26	-5.293447293447294
11	27	-5.388888888888889
11	28	-5.305840455840455
11	29	-4.623076923076924
11	30	-4.702421652421654
11	31	-4.994586894586895
11	32	-4.8901709401709414
11	33	-4.956410256410258
11	34	-5.092307692307694
11	35	-4.913105413105415
11	36	-5.648005698005695
11	37	-5.566381766381767
11	38	-5.498860398860396
11	39	-5.382478632478632
11	40	-5.254700854700854
12	1	1.8735042735042775
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109	4	3.2692307692307665
109	5	1.8518518518518547
109	6	10.346581196581194
109	7	2.0782051282051235
109	8	0.4280626780626804
109	9	3.219658119658117
109	10	3.583903133903135
109	11	1.4762108262108242
109	12	0.09301994301993943
109	13	-0.6954415954415989
109	14	-1.7226495726495727
109	15	6.073361823361822
109	16	-0.6386039886039896
109	17	-0.7330484330484328
109	18	-1.126495726495726
109	19	-5.430911680911682
109	20	0.6397435897435884
109	21	-6.298005698005699
109	22	-6.888319088319086
109	23	-6.016809116809119
109	24	-5.657692307692308
109	25	-5.833475783475784
109	26	-5.293447293447294
109	27	-5.388888888888889
109	28	-5.305840455840455
109	29	-4.623076923076924
109	30	-4.702421652421654
109	31	-4.994586894586895
109	32	-4.8901709401709414
109	33	-4.956410256410258
109	34	-5.092307692307694
109	35	-4.913105413105415
109	36	-5.648005698005695
109	37	-5.566381766381767
109	38	-5.498860398860396
109	39	-5.382478632478632
109	40	-5.254700854700854
110	1	1.9568376068376132
110	2	4.739031339031346
110	3	2.550712250712248
110	4	-9.064102564102567
110	5	-1.1481481481481453
110	6	1.6799145299145302
110	7	-3.5884615384615426
110	8	-4.9052706552706535
110	9	-6.780341880341884
110	10	-4.082763532763531
110	11	-0.8571225071225079
110	12	-2.5736467236467266
110	13	-1.028774928774931
110	14	-10.389316239316239
110	15	-9.926638176638178
110	16	-9.305270655270656
110	17	-9.399715099715099
110	18	-9.126495726495726
110	19	-8.097578347578349
110	20	-8.026923076923078
110	21	-6.298005698005699
110	22	-6.888319088319086
110	23	-6.016809116809119
110	24	-5.657692307692308
110	25	-5.833475783475784
110	26	-5.293447293447294
110	27	-5.388888888888889
110	28	-5.305840455840455
110	29	-4.623076923076924
110	30	-4.702421652421654
110	31	-4.994586894586895
110	32	-4.8901709401709414
110	33	-4.956410256410258
110	34	-5.092307692307694
110	35	-4.913105413105415
110	36	-5.648005698005695
110	37	-5.566381766381767
110	38	-5.498860398860396
110	39	-5.382478632478632
110	40	-5.254700854700854
111	1	1.1235042735042775
111	2	5.072364672364678
111	3	7.88404558404558
111	4	-9.397435897435901
111	5	-6.481481481481477
111	6	-2.4867521367521377
111	7	-11.255128205128209
111	8	-9.905270655270654
111	9	-10.44700854700855
111	10	-7.082763532763531
111	11	-10.190455840455842
111	12	-9.90698005698006
111	13	-10.362108262108265
111	14	-10.389316239316239
111	15	-9.926638176638178
111	16	-9.305270655270656
111	17	-9.399715099715099
111	18	-9.126495726495726
111	19	-8.097578347578349
111	20	-8.026923076923078
111	21	-6.298005698005699
111	22	-6.888319088319086
111	23	-6.016809116809119
111	24	-5.657692307692308
111	25	-5.833475783475784
111	26	-5.293447293447294
111	27	-5.388888888888889
111	28	-5.305840455840455
111	29	-4.623076923076924
111	30	-4.702421652421654
111	31	-4.994586894586895
111	32	-4.8901709401709414
111	33	-4.956410256410258
111	34	-5.092307692307694
111	35	-4.913105413105415
111	36	-5.648005698005695
111	37	-5.566381766381767
111	38	-5.498860398860396
111	39	-5.382478632478632
111	40	-5.254700854700854
112	1	0.6235042735042775
112	2	-14.260968660968656
112	3	-1.7826210826210875
112	4	-1.0641025641025692
112	5	-2.8148148148148113
112	6	-4.320085470085472
112	7	2.7448717948717913
112	8	-4.57193732193732
112	9	2.5529914529914493
112	10	6.917236467236469
112	11	0.8095441595441581
112	12	0.7596866096866055
112	13	2.637891737891735
112	14	0.27735042735042725
112	15	-0.2599715099715123
112	16	7.694729344729344
112	17	10.933618233618233
112	18	9.206837606837606
112	19	-0.43091168091168264
112	20	2.6397435897435884
112	21	-2.6313390313390324
112	22	2.1116809116809137
112	23	-2.350142450142452
112	24	-1.657692307692308
112	25	-0.5001424501424498
112	26	-2.6267806267806266
112	27	-3.7222222222222223
112	28	-4.305840455840455
112	29	-1.2897435897435914
112	30	-0.7024216524216538
112	31	-1.3279202279202282
112	32	3.4431623931623925
112	33	-0.9564102564102583
112	34	-1.0923076923076938
112	35	-2.2464387464387476
112	36	-2.3146723646723624
112	37	4.100284900284899
112	38	-0.4988603988603959
112	39	3.284188034188034
112	40	4.411965811965812
113	1	1.6235042735042775
113	2	-2.927635327635322
113	3	0.6340455840455803
113	4	-6.897435897435901
113	5	-4.981481481481477
113	6	-3.4867521367521377
113	7	-1.2551282051282087
113	8	-6.1552706552706535
113	9	-1.1970085470085507
113	10	0.417236467236469
113	11	-4.690455840455842
113	12	-0.15698005698006057
113	13	-0.862108262108265
113	14	-3.139316239316239
113	15	-1.6766381766381784
113	16	-2.0552706552706557
113	17	2.350284900284901
113	18	-1.626495726495726
113	19	-1.0975783475783487
113	20	2.2230769230769223
113	21	7.451994301994301
113	22	7.611680911680914
113	23	8.23319088319088
113	24	8.342307692307692
113	25	5.666524216524216
113	26	7.206552706552706
113	27	7.361111111111111
113	28	10.194159544159545
113	29	10.126923076923076
113	30	10.047578347578346
113	31	7.505413105413105
113	32	9.109829059829059
113	33	9.043589743589742
113	34	5.907692307692306
113	35	9.836894586894585
113	36	9.601994301994305
113	37	7.183618233618233
113	38	8.001139601139604
113	39	10.117521367521368
113	40	4.995299145299146
114	1	1.6235042735042775
114	2	4.072364672364678
114	3	-4.7826210826210875
114	4	-1.0641025641025692
114	5	0.18518518518519045
114	6	4.3465811965811945
114	7	5.0782051282051235
114	8	3.0947293447293465
114	9	8.219658119658117
114	10	1.250569800569803
114	11	5.809544159544158
114	12	8.426353276353272
114	13	3.304558404558403
114	14	7.944017094017093
114	15	7.7400284900284895
114	16	9.028062678062676
114	17	9.600284900284901
114	18	8.873504273504274
114	19	13.235754985754983
114	20	12.63974358974359
114	21	-6.298005698005699
114	22	-6.888319088319086
114	23	-6.016809116809119
114	24	-5.657692307692308
114	25	-5.833475783475784
114	26	-5.293447293447294
114	27	-5.388888888888889
114	28	-5.305840455840455
114	29	-4.623076923076924
114	30	-4.702421652421654
114	31	-4.994586894586895
114	32	-4.8901709401709414
114	33	-4.956410256410258
114	34	-5.092307692307694
114	35	-4.913105413105415
114	36	-5.648005698005695
114	37	-5.566381766381767
114	38	-5.498860398860396
114	39	-5.382478632478632
114	40	-5.254700854700854
115	1	1.4568376068376132
115	2	5.905698005698014
115	3	-1.4492877492877518
115	4	2.1025641025640986
115	5	6.351851851851855
115	6	12.513247863247862
115	7	13.91153846153846
115	8	12.094729344729346
115	9	15.886324786324781
115	10	9.917236467236469
115	11	12.809544159544158
115	12	17.59301994301994
115	13	11.637891737891735
115	14	11.610683760683761
115	15	12.073361823361822
115	16	13.194729344729344
115	17	12.600284900284901
115	18	9.206837606837606
115	19	14.56908831908832
115	20	8.306410256410254
115	21	-2.1313390313390332
115	22	-2.5549857549857524
115	23	-0.6834757834757852
115	24	-3.157692307692308
115	25	2.3331908831908823
115	26	-3.6267806267806266
115	27	-2.2222222222222223
115	28	-1.472507122507122
115	29	-0.7897435897435914
115	30	-2.7024216524216538
115	31	-1.9945868945868952
115	32	-1.0568376068376084
115	33	0.3769230769230756
115	34	-0.9256410256410277
115	35	-0.7464387464387485
115	36	4.6853276353276385
115	37	4.766951566951567
115	38	5.834472934472938
115	39	5.450854700854702
115	40	6.07863247863248
116	1	0.6235042735042775
116	2	-8.59430199430199
116	3	0.21737891737891246
116	4	-4.064102564102569
116	5	8.518518518518523
116	6	3.3465811965811945
116	7	10.744871794871791
116	8	8.094729344729346
116	9	13.55299145299145
116	10	-0.08276353276353099
116	11	6.809544159544158
116	12	10.759686609686607
116	13	4.971225071225067
116	14	13.944017094017093
116	15	5.7400284900284895
116	16	6.361396011396012
116	17	6.266951566951569
116	18	8.206837606837606
116	19	-0.7642450142450148
116	20	4.63974358974359
116	21	5.035327635327635
116	22	2.4450142450142476
116	23	10.649857549857549
116	24	-3.324358974358975
116	25	-0.5001424501424498
116	26	-4.9601139601139606
116	27	-0.7222222222222232
116	28	-0.639173789173789
116	29	4.376923076923076
116	30	-1.3690883190883207
116	31	-2.994586894586895
116	32	-0.8901709401709414
116	33	-1.6230769230769253
116	34	-4.092307692307694
116	35	-3.5797720797720816
116	36	4.351994301994305
116	37	3.1002849002848993
116	38	4.501139601139604
116	39	4.617521367521368
116	40	4.745299145299146
117	1	-1.3764957264957225
117	2	-22.927635327635322
117	3	-21.11595441595442
117	4	-16.3974358974359
117	5	-13.481481481481477
117	6	-15.986752136752138
117	7	-11.255128205128209
117	8	-9.905270655270654
117	9	-10.44700854700855
117	10	-7.082763532763531
117	11	-10.190455840455842
117	12	-9.90698005698006
117	13	-10.362108262108265
117	14	-10.389316239316239
117	15	-9.926638176638178
117	16	-9.305270655270656
117	17	-9.399715099715099
117	18	-9.126495726495726
117	19	-8.097578347578349
117	20	-8.026923076923078
117	21	-6.298005698005699
117	22	-6.888319088319086
117	23	-6.016809116809119
117	24	-5.657692307692308
117	25	-5.833475783475784
117	26	-5.293447293447294
117	27	-5.388888888888889
117	28	-5.305840455840455
117	29	-4.623076923076924
117	30	-4.702421652421654
117	31	-4.994586894586895
117	32	-4.8901709401709414
117	33	-4.956410256410258
117	34	-5.092307692307694
117	35	-4.913105413105415
117	36	-5.648005698005695
117	37	-5.566381766381767
117	38	-5.498860398860396
117	39	-5.382478632478632
117	40	-5.254700854700854
118	1	-3.3764957264957225
118	2	1.7390313390313459
118	3	-12.782621082621086
118	4	-12.397435897435901
118	5	-10.148148148148145
118	6	-9.986752136752138
118	7	-2.5884615384615426
118	8	-9.905270655270654
118	9	-10.44700854700855
118	10	-7.082763532763531
118	11	-10.190455840455842
118	12	-9.90698005698006
118	13	-10.362108262108265
118	14	-10.389316239316239
118	15	-9.926638176638178
118	16	-9.305270655270656
118	17	-9.399715099715099
118	18	-9.126495726495726
118	19	-8.097578347578349
118	20	-8.026923076923078
118	21	-6.298005698005699
118	22	-6.888319088319086
118	23	-6.016809116809119
118	24	-5.657692307692308
118	25	-5.833475783475784
118	26	-5.293447293447294
118	27	-5.388888888888889
118	28	-5.305840455840455
118	29	-4.623076923076924
118	30	-4.702421652421654
118	31	-4.994586894586895
118	32	-4.8901709401709414
118	33	-4.956410256410258
118	34	-5.092307692307694
118	35	-4.913105413105415
118	36	-5.648005698005695
118	37	-5.566381766381767
118	38	-5.498860398860396
118	39	-5.382478632478632
118	40	-5.254700854700854
119	1	-13.376495726495722
119	2	-8.427635327635322
119	3	-12.36595441595442
119	4	-9.647435897435901
119	5	-4.231481481481477
119	6	-3.7367521367521377
119	7	-0.5051282051282087
119	8	-3.1552706552706535
119	9	0.05299145299144925
119	10	-1.332763532763531
119	11	0.8095441595441581
119	12	2.3430199430199394
119	13	1.387891737891735
119	14	1.1106837606837612
119	15	1.3233618233618216
119	16	3.9447293447293443
119	17	6.600284900284901
119	18	6.373504273504274
119	19	7.902421652421651
119	20	-1.0269230769230777
119	21	0.20199430199430068
119	22	0.11168091168091365
119	23	-4.016809116809119
119	24	-1.407692307692308
119	25	0.16652421652421623
119	26	2.7065527065527064
119	27	-0.13888888888888928
119	28	1.444159544159545
119	29	1.8769230769230756
119	30	2.7975783475783462
119	31	1.5054131054131048
119	32	2.1098290598290586
119	33	-0.20641025641025834
119	34	1.9076923076923062
119	35	-4.913105413105415
119	36	-5.648005698005695
119	37	-5.566381766381767
119	38	-5.498860398860396
119	39	-5.382478632478632
119	40	-5.254700854700854
120	1	-1.3764957264957225
120	2	3.072364672364678
120	3	-18.11595441595442
120	4	-13.397435897435901
120	5	-7.481481481481477
120	6	9.013247863247862
120	7	-9.255128205128209
120	8	-6.9052706552706535
120	9	-1.4470085470085507
120	10	15.917236467236469
120	11	8.809544159544158
120	12	-5.906980056980061
120	13	-0.36210826210826497
120	14	6.610683760683761
120	15	2.0733618233618216
120	16	-1.3052706552706557
120	17	-3.399715099715099
120	18	5.873504273504274
120	19	-2.0975783475783487
120	20	-0.026923076923077716
120	21	11.7019943019943
120	22	7.111680911680914
120	23	0.9831908831908809
120	24	-1.657692307692308
120	25	12.166524216524216
120	26	16.706552706552706
120	27	23.61111111111111
120	28	8.694159544159545
120	29	20.376923076923077
120	30	18.297578347578344
120	31	26.005413105413105
120	32	26.10982905982906
120	33	27.04358974358974
120	34	25.907692307692308
120	35	26.086894586894587
120	36	25.351994301994303
120	37	25.433618233618233
120	38	25.501139601139606
120	39	25.61752136752137
120	40	25.745299145299146
>>END_MODULE
>>Per sequence quality scores	fail
#Quality	Count
4	365.0
5	282.0
6	201.0
7	261.0
8	138.0
9	56.0
10	112.0
11	105.0
12	105.0
13	136.0
14	142.0
15	126.0
16	110.0
17	117.0
18	101.0
19	91.0
20	81.0
21	86.0
22	108.0
23	89.0
24	100.0
25	78.0
26	77.0
27	45.0
28	35.0
29	22.0
30	11.0
31	5.0
32	7.0
33	4.0
34	1.0
35	1.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.0	0.0	0.0	100.0
2	0.0	0.0	0.0	100.0
3	0.0	0.0	100.0	0.0
4	0.0	0.0	100.0	0.0
5	0.0	0.0	100.0	0.0
6	0.0	0.0	0.0	100.0
7	98.24835783547076	0.7507037847982484	0.3440725680325305	0.6568658116984674
8	96.77621283255085	0.6885758998435054	0.7824726134585289	1.7527386541471048
9	96.24648107600876	1.563966218329684	1.0634970284641851	1.1260556771973727
10	41.40087554721701	33.270794246404	11.038148843026892	14.290181363352094
11	23.858661663539714	30.112570356472794	24.140087554721703	21.88868042526579
12	34.052532833020635	17.041901188242655	24.202626641651033	24.702939337085677
13	26.82926829268293	17.13570981863665	31.769856160100062	24.265165728580364
14	18.29268292682927	25.95372107567229	32.36397748592871	23.389618511569733
15	22.607879924953096	27.048155096935584	28.424015009380867	21.919949968730457
16	21.88868042526579	23.483427141963727	30.143839899937465	24.48405253283302
17	20.950594121325828	24.984365228267666	25.234521575984992	28.830519074421513
18	22.201375859912446	27.173233270794245	20.32520325203252	30.30018761726079
19	16.766091051805336	18.83830455259027	19.529042386185242	44.866562009419155
20	22.326454033771107	26.32895559724828	25.203252032520325	26.14133833646029
21	25.203252032520325	23.327079424640402	23.796122576610383	27.67354596622889
22	21.450906816760476	20.26266416510319	21.544715447154474	36.741713570981865
23	21.200750469043154	26.516572858036273	28.392745465916196	23.889931207004377
24	20.32520325203252	26.20387742338962	27.07942464040025	26.391494684177612
25	22.08320300281514	28.90209571473256	25.68032530497341	23.334375977478885
26	17.03331238214959	28.1269641734758	32.14959145191703	22.690131992457573
27	17.516421645292464	22.489834219580857	31.1854863934939	28.80825774163278
28	16.359086643728496	23.928683140444164	32.46793869252424	27.244291523303097
29	13.516896120150188	22.215269086357946	34.85607008760952	29.411764705882355
30	11.987480438184663	23.661971830985916	35.08607198748044	29.26447574334898
31	11.58422041327489	27.551659361302445	38.2592360676268	22.60488415779587
32	15.040650406504067	23.233270794246405	38.524077548467794	23.20200125078174
33	11.667187988739443	24.80450422270879	40.412887081639035	23.115420706912733
34	11.851156973108193	25.01563477173233	43.683552220137585	19.44965603502189
35	12.300469483568074	23.286384976525824	42.19092331768388	22.22222222222222
36	9.921752738654147	23.129890453834115	41.94053208137715	25.007824726134587
37	9.207641716254306	24.491074224866896	42.56185405574694	23.73943000313185
38	9.193245778611631	25.57848655409631	42.93308317698561	22.29518449030644
39	11.76470588235294	25.81351689612015	41.896120150187734	20.52565707133917
40	9.34959349593496	27.892432770481552	42.12007504690432	20.637898686679172
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	1.0
15	2.0
16	5.5
17	9.0
18	9.0
19	22.0
20	35.0
21	47.5
22	60.0
23	60.0
24	65.0
25	70.0
26	85.0
27	100.0
28	100.0
29	106.0
30	112.0
31	104.5
32	97.0
33	97.0
34	115.5
35	134.0
36	140.5
37	147.0
38	147.0
39	176.0
40	205.0
41	218.0
42	231.0
43	231.0
44	232.0
45	233.0
46	220.0
47	207.0
48	207.0
49	203.5
50	200.0
51	185.0
52	170.0
53	170.0
54	168.5
55	167.0
56	181.0
57	195.0
58	195.0
59	180.5
60	166.0
61	160.5
62	155.0
63	155.0
64	149.0
65	143.0
66	131.0
67	119.0
68	119.0
69	111.5
70	104.0
71	88.0
72	72.0
73	72.0
74	49.5
75	27.0
76	22.5
77	18.0
78	18.0
79	14.5
80	11.0
81	7.0
82	3.0
83	3.0
84	2.5
85	2.0
86	1.5
87	1.0
88	1.0
89	2.0
90	3.0
91	1.5
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.031269543464665414
8	0.09380863039399624
9	0.031269543464665414
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.40650406504065045
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.031269543464665414
26	0.5003126954346466
27	0.031269543464665414
28	0.031269543464665414
29	0.06253908692933083
30	0.09380863039399624
31	0.12507817385866166
32	0.0
33	0.031269543464665414
34	0.0
35	0.09380863039399624
36	0.09380863039399624
37	0.15634771732332708
38	0.0
39	0.06253908692933083
40	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
40	3198.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	94.87179487179486
#Duplication Level	Percentage of deduplicated	Percentage of total
1	96.47330257086355	91.52595372107567
2	2.8345418589321025	5.378361475922452
3	0.2966381015161503	0.8442776735459663
4	0.1977587343441002	0.7504690431519699
5	0.06591957811470006	0.31269543464665417
6	0.06591957811470006	0.37523452157598497
7	0.0	0.0
8	0.0	0.0
9	0.0	0.0
>10	0.06591957811470006	0.8130081300813009
>50	0.0	0.0
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CCTTTCGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTT	14	0.4377736085053158	No Hit
CCTTTCGGGGATGTTGTCCTGGCTGTTGCCAAAAAAAAAA	12	0.37523452157598497	No Hit
CCTTTCGGGATGTTGTCCTGGCTGTTGCCAAAAAAAAAAA	6	0.18761726078799248	No Hit
CCTTTCGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTT	6	0.18761726078799248	No Hit
CCTTTCGGGCTCGTCTTAATCAGCAACATCTTTTGATACA	5	0.15634771732332708	No Hit
CCTTTCGGGGCCGAATAGCCTCTCCACCCCAGCGGCCGGA	5	0.15634771732332708	No Hit
CCTTTCGGGCTCTTCGGACTTGGGCTTGGGTTCCGGCGAC	4	0.12507817385866166	No Hit
CCTTTCGGGGATGTTGTCCTGGCTGTTGGGAAAAAAAAAA	4	0.12507817385866166	No Hit
CCTTTCGGGGCCGAATAGCCTCTCCACCCCAGCCGCCCGC	4	0.12507817385866166	No Hit
CCTTTCGGGGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4	0.12507817385866166	No Hit
CCTTTCGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	4	0.12507817385866166	No Hit
CCTTTCGGGAAGCATGTATCAACGCAGAGTCGACTTTTTT	4	0.12507817385866166	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.031269543464665414	0.0	0.0	0.0	0.0
13	0.031269543464665414	0.0	0.0	0.0	0.0
14	0.031269543464665414	0.0	0.0	0.0	0.0
15	0.06253908692933083	0.0	0.0	0.0	0.0
16	0.06253908692933083	0.0	0.0	0.0	0.0
17	0.09380863039399624	0.0	0.0	0.0	0.0
18	0.09380863039399624	0.0	0.0	0.0	0.0
19	0.09380863039399624	0.0	0.0	0.0	0.0
20	0.09380863039399624	0.0	0.0	0.0	0.0
21	0.09380863039399624	0.0	0.0	0.0	0.0
22	0.09380863039399624	0.0	0.0	0.0	0.0
23	0.09380863039399624	0.0	0.0	0.0	0.0
24	0.09380863039399624	0.0	0.0	0.0	0.0
25	0.09380863039399624	0.0	0.0	0.0	0.0
26	0.09380863039399624	0.0	0.0	0.0	0.0
27	0.09380863039399624	0.0	0.0	0.0	0.0
28	0.09380863039399624	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGGGGAT	25	1.6068063E-4	34.0	6
CGGGATG	65	0.0	34.0	6
CTGTTGC	30	1.0692087E-5	34.0	22
TTCGGGT	25	1.6068063E-4	34.0	4
TTCGGGG	130	0.0	34.0	4
TTCGGGA	125	0.0	34.0	4
TTTCGGG	295	0.0	34.0	3
TCGGGGT	35	7.0350325E-7	34.0	5
TCGGGGG	35	7.0350325E-7	34.0	5
TCGGGGC	20	0.0023896128	34.0	5
TCGGGGA	40	4.5722118E-8	34.0	5
TCGGGAT	75	0.0	34.0	5
TCGGGAA	35	7.0350325E-7	34.0	5
CCTTTCG	320	0.0	33.46875	1
CTTTCGG	315	0.0	33.46032	2
GCTGTTG	40	2.0011503E-6	29.75	21
GGCTGTT	50	1.1394946E-5	23.8	20
TGGCTGT	50	1.1394946E-5	23.8	19
GGGATGT	65	1.0592885E-7	23.538462	7
TTGTCCT	55	2.3874163E-5	21.636362	13
>>END_MODULE