FastQCFastQC Report
Fri 17 Jun 2016
SRR1526176_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526176_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2499
Sequences flagged as poor quality0
Sequence length40
%GC51

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTTTCGGGGTCGACTTTTTTTTTTTTTTTTTTTTTTTTT110.4401760704281713No Hit
CCTTTCGGGGTCGCCTTTTTTTTTTTTTTTTTTTTTTTTT90.36014405762304924No Hit
CCTTTCGGGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTT40.1600640256102441No Hit
CCTTTCGGGTTCGCTCGTTTTTTTTTTTTTTTTTTTTTTT40.1600640256102441No Hit
CCTTTCGGGATCCGCCTTTTTTTTTTTTTTTTTTTTTTTT30.12004801920768307No Hit
CCTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30.12004801920768307No Hit
CCTTTCGGGTCGCCTTTTTTTTTTTTTTTTTTTTTTTTTT30.12004801920768307No Hit
CCTTTCGGGGTCACTTCTTTTTTTTTTTTTTTTTTTTTTT30.12004801920768307No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCGGGT200.0022826834.0000044
TTCGGGA800.034.0000044
TCGGGGT200.0022826834.0000045
TCGGGGC200.0022826834.0000045
TCGGGAT309.688099E-634.0000045
TCGGGAG200.0022826834.0000045
TCGGGAC200.0022826834.0000045
CCTTTCG2300.034.01
TTCGGGG559.094947E-1234.04
TTCGGGC650.034.04
TTTCGGG2200.034.03
TCGGGCT251.4988864E-434.05
CTTTCGG2250.034.02
Produced by FastQC (version 0.11.5)