##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1526176_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2499
Sequences flagged as poor quality	0
Sequence length	40
%GC	51
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	12.113645458183273	4.0	4.0	30.0	4.0	33.0
2	8.867947178871548	4.0	4.0	4.0	4.0	30.0
3	6.473389355742297	4.0	4.0	4.0	4.0	14.0
4	5.75390156062425	4.0	4.0	4.0	4.0	5.0
5	5.677070828331333	4.0	4.0	4.0	4.0	5.0
6	5.995598239295719	4.0	4.0	4.0	4.0	4.0
7	4.945178071228491	4.0	4.0	4.0	4.0	4.0
8	5.0056022408963585	4.0	4.0	4.0	4.0	4.0
9	4.875150060024009	4.0	4.0	4.0	4.0	4.0
10	4.932372949179672	4.0	4.0	4.0	4.0	4.0
11	4.959983993597439	4.0	4.0	4.0	4.0	4.0
12	5.049619847939176	4.0	4.0	4.0	4.0	4.0
13	4.964385754301721	4.0	4.0	4.0	4.0	4.0
14	4.941976790716287	4.0	4.0	4.0	4.0	4.0
15	4.888355342136855	4.0	4.0	4.0	4.0	4.0
16	5.00360144057623	4.0	4.0	4.0	4.0	4.0
17	4.951980792316927	4.0	4.0	4.0	4.0	4.0
18	4.840336134453781	4.0	4.0	4.0	4.0	4.0
19	4.891156462585034	4.0	4.0	4.0	4.0	4.0
20	4.895558223289315	4.0	4.0	4.0	4.0	4.0
21	5.064825930372149	4.0	4.0	4.0	4.0	4.0
22	5.070828331332533	4.0	4.0	4.0	4.0	4.0
23	5.024009603841536	4.0	4.0	4.0	4.0	4.0
24	5.002801120448179	4.0	4.0	4.0	4.0	4.0
25	4.957583033213285	4.0	4.0	4.0	4.0	4.0
26	5.138455382152861	4.0	4.0	4.0	4.0	4.0
27	5.119647859143657	4.0	4.0	4.0	4.0	4.0
28	5.101640656262505	4.0	4.0	4.0	4.0	4.0
29	5.051620648259304	4.0	4.0	4.0	4.0	4.0
30	5.086834733893557	4.0	4.0	4.0	4.0	4.0
31	5.085634253701481	4.0	4.0	4.0	4.0	4.0
32	5.057623049219688	4.0	4.0	4.0	4.0	4.0
33	5.059623849539816	4.0	4.0	4.0	4.0	4.0
34	5.056022408963585	4.0	4.0	4.0	4.0	4.0
35	5.011204481792717	4.0	4.0	4.0	4.0	4.0
36	4.877551020408164	4.0	4.0	4.0	4.0	4.0
37	4.841936774709884	4.0	4.0	4.0	4.0	4.0
38	4.87234893957583	4.0	4.0	4.0	4.0	4.0
39	4.879551820728292	4.0	4.0	4.0	4.0	4.0
40	4.848739495798319	4.0	4.0	4.0	4.0	4.0
>>END_MODULE
>>Per tile sequence quality	fail
#Tile	Base	Mean
1	1	4.101415094339622
1	2	3.1685534591194973
1	3	2.768396226415092
1	4	9.767138364779875
1	5	4.680817610062892
1	6	0.5668238993710695
1	7	1.0468553459119496
1	8	-0.17327044025157168
1	9	-0.10581761006289447
1	10	0.2792452830188692
1	11	3.3613207547169814
1	12	3.093553459119497
1	13	3.4268867924528292
1	14	2.2264150943396235
1	15	3.270440251572327
1	16	4.008647798742139
1	17	4.072327044025157
1	18	3.7177672955974845
1	19	3.64937106918239
1	20	4.235849056603774
1	21	-1.1139937106918234
1	22	-1.0377358490566042
1	23	-1.0613207547169807
1	24	-0.8742138364779874
1	25	-0.9386792452830193
1	26	-1.3922955974842761
1	27	-1.4433962264150946
1	28	-1.416666666666667
1	29	-1.4449685534591206
1	30	-1.4819182389937096
1	31	-0.9952830188679247
1	32	-0.867924528301887
1	33	-1.0770440251572335
1	34	-1.0408805031446535
1	35	-1.0204402515723263
1	36	-0.9905660377358494
1	37	-0.9748427672955966
1	38	-0.9323899371069171
1	39	-1.0110062893081766
1	40	-1.033018867924528
3	1	18.529986522911052
3	2	-4.260017969451932
3	3	-1.9458894878706214
3	4	-1.232861635220126
3	5	-1.0334681042228215
3	6	-1.5760332434860738
3	7	-0.9531446540880504
3	8	-1.0304132973944284
3	9	-0.9629604672057512
3	10	-0.863611859838274
3	11	-0.7815363881401609
3	12	-1.0493036837376453
3	13	-0.7159703504043131
3	14	-1.059299191374662
3	15	-0.8724168912848151
3	16	-0.8484950584007187
3	17	-0.7848158131177003
3	18	-0.7108041329739443
3	19	-0.7792003593890389
3	20	-0.6212938005390836
3	21	-1.1139937106918234
3	22	-1.0377358490566042
3	23	-1.0613207547169807
3	24	-0.8742138364779874
3	25	-0.9386792452830193
3	26	-1.3922955974842761
3	27	-1.4433962264150946
3	28	-1.416666666666667
3	29	-1.4449685534591206
3	30	-1.4819182389937096
3	31	-0.9952830188679247
3	32	-0.867924528301887
3	33	-1.0770440251572335
3	34	-1.0408805031446535
3	35	-1.0204402515723263
3	36	-0.9905660377358494
3	37	-0.9748427672955966
3	38	-0.9323899371069171
3	39	-1.0110062893081766
3	40	-1.033018867924528
4	1	-7.4700134770889495
4	2	-4.260017969451932
4	3	-1.9458894878706214
4	4	-1.232861635220126
4	5	-1.0334681042228215
4	6	-1.5760332434860738
4	7	-0.9531446540880504
4	8	-1.0304132973944284
4	9	-0.9629604672057512
4	10	-0.863611859838274
4	11	-0.7815363881401609
4	12	-1.0493036837376453
4	13	-0.7159703504043131
4	14	-1.059299191374662
4	15	-0.8724168912848151
4	16	-0.8484950584007187
4	17	-0.7848158131177003
4	18	-0.7108041329739443
4	19	-0.7792003593890389
4	20	-0.6212938005390836
4	21	-1.1139937106918234
4	22	-1.0377358490566042
4	23	-1.0613207547169807
4	24	-0.8742138364779874
4	25	-0.9386792452830193
4	26	-1.3922955974842761
4	27	-1.4433962264150946
4	28	-1.416666666666667
4	29	-1.4449685534591206
4	30	-1.4819182389937096
4	31	-0.9952830188679247
4	32	-0.867924528301887
4	33	-1.0770440251572335
4	34	-1.0408805031446535
4	35	-1.0204402515723263
4	36	-0.9905660377358494
4	37	-0.9748427672955966
4	38	-0.9323899371069171
4	39	-1.0110062893081766
4	40	-1.033018867924528
5	1	-7.4700134770889495
5	2	-4.260017969451932
5	3	-1.9458894878706214
5	4	-1.232861635220126
5	5	-1.0334681042228215
5	6	-1.5760332434860738
5	7	-0.9531446540880504
5	8	-1.0304132973944284
5	9	-0.9629604672057512
5	10	-0.863611859838274
5	11	-0.7815363881401609
5	12	-1.0493036837376453
5	13	-0.7159703504043131
5	14	-1.059299191374662
5	15	-0.8724168912848151
5	16	-0.8484950584007187
5	17	-0.7848158131177003
5	18	-0.7108041329739443
5	19	-0.7792003593890389
5	20	-0.6212938005390836
5	21	-1.1139937106918234
5	22	-1.0377358490566042
5	23	-1.0613207547169807
5	24	-0.8742138364779874
5	25	-0.9386792452830193
5	26	-1.3922955974842761
5	27	-1.4433962264150946
5	28	-1.416666666666667
5	29	-1.4449685534591206
5	30	-1.4819182389937096
5	31	-0.9952830188679247
5	32	-0.867924528301887
5	33	-1.0770440251572335
5	34	-1.0408805031446535
5	35	-1.0204402515723263
5	36	-0.9905660377358494
5	37	-0.9748427672955966
5	38	-0.9323899371069171
5	39	-1.0110062893081766
5	40	-1.033018867924528
6	1	5.5299865229110505
6	2	-4.260017969451932
6	3	-1.9458894878706214
6	4	-1.232861635220126
6	5	-1.0334681042228215
6	6	-1.5760332434860738
6	7	-0.9531446540880504
6	8	-1.0304132973944284
6	9	-0.9629604672057512
6	10	-0.863611859838274
6	11	-0.7815363881401609
6	12	-1.0493036837376453
6	13	-0.7159703504043131
6	14	-1.059299191374662
6	15	-0.8724168912848151
6	16	-0.8484950584007187
6	17	-0.7848158131177003
6	18	-0.7108041329739443
6	19	-0.7792003593890389
6	20	-0.6212938005390836
6	21	-1.1139937106918234
6	22	-1.0377358490566042
6	23	-1.0613207547169807
6	24	-0.8742138364779874
6	25	-0.9386792452830193
6	26	-1.3922955974842761
6	27	-1.4433962264150946
6	28	-1.416666666666667
6	29	-1.4449685534591206
6	30	-1.4819182389937096
6	31	-0.9952830188679247
6	32	-0.867924528301887
6	33	-1.0770440251572335
6	34	-1.0408805031446535
6	35	-1.0204402515723263
6	36	-0.9905660377358494
6	37	-0.9748427672955966
6	38	-0.9323899371069171
6	39	-1.0110062893081766
6	40	-1.033018867924528
8	1	-7.4700134770889495
8	2	-4.260017969451932
8	3	-1.9458894878706214
8	4	-1.232861635220126
8	5	-1.0334681042228215
8	6	-1.5760332434860738
8	7	-0.9531446540880504
8	8	-1.0304132973944284
8	9	-0.9629604672057512
8	10	-0.863611859838274
8	11	-0.7815363881401609
8	12	-1.0493036837376453
8	13	-0.7159703504043131
8	14	-1.059299191374662
8	15	-0.8724168912848151
8	16	-0.8484950584007187
8	17	-0.7848158131177003
8	18	-0.7108041329739443
8	19	-0.7792003593890389
8	20	-0.6212938005390836
8	21	-1.1139937106918234
8	22	-1.0377358490566042
8	23	-1.0613207547169807
8	24	-0.8742138364779874
8	25	-0.9386792452830193
8	26	-1.3922955974842761
8	27	-1.4433962264150946
8	28	-1.416666666666667
8	29	-1.4449685534591206
8	30	-1.4819182389937096
8	31	-0.9952830188679247
8	32	-0.867924528301887
8	33	-1.0770440251572335
8	34	-1.0408805031446535
8	35	-1.0204402515723263
8	36	-0.9905660377358494
8	37	-0.9748427672955966
8	38	-0.9323899371069171
8	39	-1.0110062893081766
8	40	-1.033018867924528
9	1	18.196653189577717
9	2	21.406648697214735
9	3	1.3874438454627116
9	4	0.100471698113207
9	5	0.6331985624438454
9	6	6.090633423180592
9	7	0.38018867924528266
9	8	0.9695867026055716
9	9	1.3703728661275818
9	10	0.8030548068283929
9	11	0.2184636118598391
9	12	7.284029649595689
9	13	1.2840296495956869
9	14	0.27403414195867093
9	15	7.460916442048519
9	16	1.8181716082659483
9	17	3.5485175202156336
9	18	1.6225292003593887
9	19	1.2207996406109611
9	20	1.7120395327942495
9	21	8.886006289308177
9	22	8.29559748427673
9	23	8.272012578616353
9	24	8.459119496855347
9	25	8.394654088050315
9	26	5.27437106918239
9	27	7.889937106918239
9	28	7.916666666666667
9	29	7.888364779874213
9	30	7.851415094339624
9	31	-0.9952830188679247
9	32	-0.867924528301887
9	33	-1.0770440251572335
9	34	-1.0408805031446535
9	35	-1.0204402515723263
9	36	-0.9905660377358494
9	37	-0.9748427672955966
9	38	-0.9323899371069171
9	39	-1.0110062893081766
9	40	-1.033018867924528
10	1	1.1966531895777166
10	2	0.07331536388140236
10	3	7.720777178796045
10	4	1.100471698113207
10	5	0.6331985624438454
10	6	3.0906334231805923
10	7	7.04685534591195
10	8	2.9695867026055716
10	9	8.703706199460914
10	10	4.136388140161726
10	11	1.218463611859839
10	12	8.61736298292902
10	13	9.61736298292902
10	14	8.607367475292005
10	15	9.460916442048518
10	16	5.151504941599281
10	17	7.2151841868823
10	18	2.2891958670260557
10	19	3.220799640610961
10	20	2.3787061994609164
10	21	1.8860062893081766
10	22	7.962264150943396
10	23	7.938679245283019
10	24	-0.8742138364779874
10	25	-0.9386792452830193
10	26	-1.3922955974842761
10	27	-1.4433962264150946
10	28	-1.416666666666667
10	29	-1.4449685534591206
10	30	-1.4819182389937096
10	31	-0.9952830188679247
10	32	-0.867924528301887
10	33	-1.0770440251572335
10	34	-1.0408805031446535
10	35	-1.0204402515723263
10	36	-0.9905660377358494
10	37	-0.9748427672955966
10	38	-0.9323899371069171
10	39	-1.0110062893081766
10	40	-1.033018867924528
11	1	5.0299865229110505
11	2	8.239982030548068
11	3	1.0541105121293786
11	4	5.267138364779874
11	5	2.4665318957771785
11	6	9.923966756513927
11	7	2.0468553459119496
11	8	3.4695867026055716
11	9	2.0370395327942488
11	10	2.136388140161726
11	11	2.218463611859839
11	12	11.450696316262356
11	13	-0.21597035040431312
11	14	10.440700808625337
11	15	10.627583108715186
11	16	2.1515049415992813
11	17	3.2151841868822997
11	18	0.2891958670260557
11	19	5.720799640610961
11	20	6.378706199460916
11	21	8.886006289308177
11	22	9.962264150943396
11	23	9.93867924528302
11	24	10.125786163522012
11	25	13.06132075471698
11	26	12.607704402515724
11	27	12.556603773584905
11	28	12.583333333333332
11	29	12.55503144654088
11	30	12.51808176100629
11	31	14.004716981132075
11	32	13.132075471698112
11	33	13.922955974842766
11	34	12.959119496855347
11	35	12.979559748427674
11	36	13.00943396226415
11	37	13.025157232704403
11	38	13.067610062893083
11	39	12.988993710691823
11	40	12.966981132075471
12	1	-7.4700134770889495
12	2	-4.260017969451932
12	3	-1.9458894878706214
12	4	-1.232861635220126
12	5	-1.0334681042228215
12	6	-1.5760332434860738
12	7	-0.9531446540880504
12	8	-1.0304132973944284
12	9	-0.9629604672057512
12	10	-0.863611859838274
12	11	-0.7815363881401609
12	12	-1.0493036837376453
12	13	-0.7159703504043131
12	14	-1.059299191374662
12	15	-0.8724168912848151
12	16	-0.8484950584007187
12	17	-0.7848158131177003
12	18	-0.7108041329739443
12	19	-0.7792003593890389
12	20	-0.6212938005390836
12	21	-1.1139937106918234
12	22	-1.0377358490566042
12	23	-1.0613207547169807
12	24	-0.8742138364779874
12	25	-0.9386792452830193
12	26	-1.3922955974842761
12	27	-1.4433962264150946
12	28	-1.416666666666667
12	29	-1.4449685534591206
12	30	-1.4819182389937096
12	31	-0.9952830188679247
12	32	-0.867924528301887
12	33	-1.0770440251572335
12	34	-1.0408805031446535
12	35	-1.0204402515723263
12	36	-0.9905660377358494
12	37	-0.9748427672955966
12	38	-0.9323899371069171
12	39	-1.0110062893081766
12	40	-1.033018867924528
13	1	-7.4700134770889495
13	2	-4.260017969451932
13	3	-1.9458894878706214
13	4	-1.232861635220126
13	5	-1.0334681042228215
13	6	-1.5760332434860738
13	7	-0.9531446540880504
13	8	-1.0304132973944284
13	9	-0.9629604672057512
13	10	-0.863611859838274
13	11	-0.7815363881401609
13	12	-1.0493036837376453
13	13	-0.7159703504043131
13	14	-1.059299191374662
13	15	-0.8724168912848151
13	16	-0.8484950584007187
13	17	-0.7848158131177003
13	18	-0.7108041329739443
13	19	-0.7792003593890389
13	20	-0.6212938005390836
13	21	-1.1139937106918234
13	22	-1.0377358490566042
13	23	-1.0613207547169807
13	24	-0.8742138364779874
13	25	-0.9386792452830193
13	26	-1.3922955974842761
13	27	-1.4433962264150946
13	28	-1.416666666666667
13	29	-1.4449685534591206
13	30	-1.4819182389937096
13	31	-0.9952830188679247
13	32	-0.867924528301887
13	33	-1.0770440251572335
13	34	-1.0408805031446535
13	35	-1.0204402515723263
13	36	-0.9905660377358494
13	37	-0.9748427672955966
13	38	-0.9323899371069171
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118	8	-1.0304132973944284
118	9	-0.9629604672057512
118	10	-0.863611859838274
118	11	-0.7815363881401609
118	12	-1.0493036837376453
118	13	-0.7159703504043131
118	14	-1.059299191374662
118	15	-0.8724168912848151
118	16	-0.8484950584007187
118	17	-0.7848158131177003
118	18	-0.7108041329739443
118	19	-0.7792003593890389
118	20	-0.6212938005390836
118	21	-1.1139937106918234
118	22	-1.0377358490566042
118	23	-1.0613207547169807
118	24	-0.8742138364779874
118	25	-0.9386792452830193
118	26	-1.3922955974842761
118	27	-1.4433962264150946
118	28	-1.416666666666667
118	29	-1.4449685534591206
118	30	-1.4819182389937096
118	31	-0.9952830188679247
118	32	-0.867924528301887
118	33	-1.0770440251572335
118	34	-1.0408805031446535
118	35	-1.0204402515723263
118	36	-0.9905660377358494
118	37	-0.9748427672955966
118	38	-0.9323899371069171
118	39	-1.0110062893081766
118	40	-1.033018867924528
119	1	-7.4700134770889495
119	2	-4.260017969451932
119	3	-1.9458894878706214
119	4	-1.232861635220126
119	5	-1.0334681042228215
119	6	-1.5760332434860738
119	7	-0.9531446540880504
119	8	-1.0304132973944284
119	9	-0.9629604672057512
119	10	-0.863611859838274
119	11	-0.7815363881401609
119	12	-1.0493036837376453
119	13	-0.7159703504043131
119	14	-1.059299191374662
119	15	-0.8724168912848151
119	16	-0.8484950584007187
119	17	-0.7848158131177003
119	18	-0.7108041329739443
119	19	-0.7792003593890389
119	20	-0.6212938005390836
119	21	-1.1139937106918234
119	22	-1.0377358490566042
119	23	-1.0613207547169807
119	24	-0.8742138364779874
119	25	-0.9386792452830193
119	26	-1.3922955974842761
119	27	-1.4433962264150946
119	28	-1.416666666666667
119	29	-1.4449685534591206
119	30	-1.4819182389937096
119	31	-0.9952830188679247
119	32	-0.867924528301887
119	33	-1.0770440251572335
119	34	-1.0408805031446535
119	35	-1.0204402515723263
119	36	-0.9905660377358494
119	37	-0.9748427672955966
119	38	-0.9323899371069171
119	39	-1.0110062893081766
119	40	-1.033018867924528
120	1	18.529986522911052
120	2	21.73998203054807
120	3	-1.9458894878706214
120	4	-1.232861635220126
120	5	-1.0334681042228215
120	6	-1.5760332434860738
120	7	-0.9531446540880504
120	8	-1.0304132973944284
120	9	-0.9629604672057512
120	10	-0.863611859838274
120	11	-0.7815363881401609
120	12	-1.0493036837376453
120	13	-0.7159703504043131
120	14	-1.059299191374662
120	15	-0.8724168912848151
120	16	-0.8484950584007187
120	17	-0.7848158131177003
120	18	-0.7108041329739443
120	19	-0.7792003593890389
120	20	-0.6212938005390836
120	21	-1.1139937106918234
120	22	-1.0377358490566042
120	23	-1.0613207547169807
120	24	-0.8742138364779874
120	25	-0.9386792452830193
120	26	-1.3922955974842761
120	27	-1.4433962264150946
120	28	-1.416666666666667
120	29	-1.4449685534591206
120	30	-1.4819182389937096
120	31	-0.9952830188679247
120	32	-0.867924528301887
120	33	-1.0770440251572335
120	34	-1.0408805031446535
120	35	-1.0204402515723263
120	36	-0.9905660377358494
120	37	-0.9748427672955966
120	38	-0.9323899371069171
120	39	-1.0110062893081766
120	40	-1.033018867924528
>>END_MODULE
>>Per sequence quality scores	fail
#Quality	Count
4	1998.0
5	218.0
6	49.0
7	47.0
8	16.0
9	8.0
10	10.0
11	9.0
12	4.0
13	5.0
14	11.0
15	3.0
16	7.0
17	7.0
18	11.0
19	12.0
20	10.0
21	14.0
22	15.0
23	14.0
24	11.0
25	8.0
26	7.0
27	1.0
28	1.0
29	3.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.0	0.0	0.0	100.0
2	0.0	0.0	0.0	100.0
3	0.0	0.0	100.0	0.0
4	0.0	0.0	100.0	0.0
5	0.0	0.0	100.0	0.0
6	0.0	0.0	0.0	100.0
7	96.46728221597752	0.40144520272982737	0.7226013649136892	2.408671216378964
8	97.71908763505402	0.4001600640256103	0.9203681472589036	0.9603841536614646
9	96.26955475330927	0.762133975130365	1.2835940633774567	1.684717208182912
10	34.293717486994794	28.931572629051622	10.244097639055623	26.53061224489796
11	22.488995598239296	20.328131252501	30.05202080832333	27.13085234093637
12	32.61304521808724	9.643857543017207	27.531012404961984	30.212084833933574
13	22.729091636654662	16.56662665066026	32.53301320528211	28.171268507402964
14	18.65492393915132	18.935148118494798	35.268214571657325	27.141713370696557
15	23.689475790316123	16.406562625050018	31.092436974789916	28.811524609843936
16	23.769507803121247	17.92717086834734	30.77230892356943	27.531012404961984
17	19.807923169267706	19.08763505402161	32.33293317326931	28.771508603441376
18	15.046018407362945	23.12925170068027	31.4125650260104	30.41216486594638
19	17.288407541115124	18.251103088648215	28.559967910148416	35.90052146008825
20	19.12765106042417	20.128051220488192	30.172068827531014	30.572228891556623
21	18.687474989995998	19.52781112444978	32.01280512204882	29.7719087635054
22	16.246498599439775	22.088835534213686	27.571028411364544	34.093637454981994
23	14.525810324129651	20.208083233293316	31.732693077230895	33.533413365346135
24	14.765906362545017	24.489795918367346	33.17326930772309	27.571028411364544
25	15.625	21.514423076923077	28.685897435897434	34.17467948717949
26	14.480545527476934	21.379863618130766	29.402326514239874	34.73726434015243
27	16.39871382636656	20.739549839228296	30.94855305466238	31.913183279742764
28	15.162454873646208	21.21941436020858	30.645808263136782	32.97232250300842
29	15.903614457831324	19.27710843373494	31.52610441767068	33.29317269076305
30	13.010408326661329	19.65572457966373	36.22898318654924	31.104883907125704
31	12.289831865492394	20.37630104083267	36.269015212169734	31.064851881505206
32	14.811849479583666	16.853482786228984	35.188150520416336	33.14651721377101
33	12.169735788630904	14.691753402722178	36.10888710968775	37.02962369895917
34	9.40376150460184	16.646658663465384	40.29611844737895	33.65346138455382
35	10.844337735094038	15.166066426570628	41.81672669067627	32.172869147659064
36	10.604241696678672	18.367346938775512	40.656262505002	30.372148859543817
37	11.218335343787695	18.69722557297949	42.340168878166466	27.744270205066346
38	13.250600480384309	18.815052041633308	40.072057646116896	27.862289831865496
39	12.805122048819529	18.847539015606245	39.015606242497	29.331732693077228
40	11.128903122497999	18.374699759807847	39.55164131305044	30.944755804643716
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	1.5
7	3.0
8	3.0
9	1.5
10	0.0
11	0.0
12	0.0
13	0.0
14	0.5
15	1.0
16	3.5
17	6.0
18	6.0
19	14.5
20	23.0
21	24.0
22	25.0
23	25.0
24	36.5
25	48.0
26	58.0
27	68.0
28	68.0
29	64.0
30	60.0
31	65.0
32	70.0
33	70.0
34	74.0
35	78.0
36	84.0
37	90.0
38	90.0
39	100.0
40	110.0
41	111.5
42	113.0
43	113.0
44	131.0
45	149.0
46	139.5
47	130.0
48	130.0
49	156.5
50	183.0
51	176.5
52	170.0
53	170.0
54	168.5
55	167.0
56	162.5
57	158.0
58	158.0
59	165.0
60	172.0
61	166.0
62	160.0
63	160.0
64	145.0
65	130.0
66	119.5
67	109.0
68	109.0
69	100.5
70	92.0
71	73.0
72	54.0
73	54.0
74	51.0
75	48.0
76	38.0
77	28.0
78	28.0
79	22.5
80	17.0
81	17.0
82	17.0
83	17.0
84	13.0
85	9.0
86	8.5
87	8.0
88	8.0
89	5.0
90	2.0
91	1.5
92	1.0
93	1.0
94	0.5
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.3201280512204882
8	0.0
9	0.24009603841536614
10	0.0
11	0.0
12	0.0
13	0.0
14	0.040016006402561026
15	0.0
16	0.0
17	0.0
18	0.0
19	0.24009603841536614
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.12004801920768307
26	0.24009603841536614
27	0.4401760704281713
28	0.24009603841536614
29	0.36014405762304924
30	0.040016006402561026
31	0.040016006402561026
32	0.040016006402561026
33	0.040016006402561026
34	0.0
35	0.0
36	0.0
37	0.4801920768307323
38	0.040016006402561026
39	0.0
40	0.040016006402561026
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
40	2499.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	97.79911964785914
#Duplication Level	Percentage of deduplicated	Percentage of total
1	98.7315875613748	96.55862344937975
2	0.9410801963993453	1.8407362945178072
3	0.16366612111292964	0.4801920768307323
4	0.08183306055646482	0.3201280512204882
5	0.0	0.0
6	0.0	0.0
7	0.0	0.0
8	0.0	0.0
9	0.04091653027823241	0.36014405762304924
>10	0.04091653027823241	0.4401760704281713
>50	0.0	0.0
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CCTTTCGGGGTCGACTTTTTTTTTTTTTTTTTTTTTTTTT	11	0.4401760704281713	No Hit
CCTTTCGGGGTCGCCTTTTTTTTTTTTTTTTTTTTTTTTT	9	0.36014405762304924	No Hit
CCTTTCGGGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTT	4	0.1600640256102441	No Hit
CCTTTCGGGTTCGCTCGTTTTTTTTTTTTTTTTTTTTTTT	4	0.1600640256102441	No Hit
CCTTTCGGGATCCGCCTTTTTTTTTTTTTTTTTTTTTTTT	3	0.12004801920768307	No Hit
CCTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3	0.12004801920768307	No Hit
CCTTTCGGGTCGCCTTTTTTTTTTTTTTTTTTTTTTTTTT	3	0.12004801920768307	No Hit
CCTTTCGGGGTCACTTCTTTTTTTTTTTTTTTTTTTTTTT	3	0.12004801920768307	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	0.0
15	0.0	0.0	0.0	0.0	0.0
16	0.0	0.0	0.0	0.0	0.0
17	0.0	0.0	0.0	0.0	0.0
18	0.0	0.0	0.0	0.0	0.0
19	0.0	0.0	0.0	0.0	0.0
20	0.0	0.0	0.0	0.0	0.0
21	0.0	0.0	0.0	0.0	0.0
22	0.0	0.0	0.0	0.0	0.0
23	0.0	0.0	0.0	0.0	0.0
24	0.0	0.0	0.0	0.0	0.0
25	0.0	0.0	0.0	0.0	0.0
26	0.0	0.0	0.0	0.0	0.0
27	0.0	0.0	0.0	0.0	0.0
28	0.0	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTCGGGT	20	0.00228268	34.000004	4
TTCGGGA	80	0.0	34.000004	4
TCGGGGT	20	0.00228268	34.000004	5
TCGGGGC	20	0.00228268	34.000004	5
TCGGGAT	30	9.688099E-6	34.000004	5
TCGGGAG	20	0.00228268	34.000004	5
TCGGGAC	20	0.00228268	34.000004	5
CCTTTCG	230	0.0	34.0	1
TTCGGGG	55	9.094947E-12	34.0	4
TTCGGGC	65	0.0	34.0	4
TTTCGGG	220	0.0	34.0	3
TCGGGCT	25	1.4988864E-4	34.0	5
CTTTCGG	225	0.0	34.0	2
>>END_MODULE