FastQCFastQC Report
Fri 17 Jun 2016
SRR1526175_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526175_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2608
Sequences flagged as poor quality0
Sequence length40
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTTTCGGGTCACAGATCCTCTTCTGAGATGAGTTTTTGT762.9141104294478524No Hit
CCTTTCGGGTCACAGATCCTCTTCTGAAGGAGATTGGGCG461.763803680981595No Hit
CCTTTCGGGTCAAAGATCCTCTTCTGAGATGAGTTTTTGT170.651840490797546No Hit
CCTTTCGGGTCAAAGATCCTCTTCTGAAGGAGATTGGGCG90.3450920245398773No Hit
CCTTTCGGTCACAGATCCTCTTCTGAGATGAGTTTTTGTT90.3450920245398773No Hit
CCTTTCGGGGTCTGTCCGTCCGTGGAGCCAGTCCTAGCCA90.3450920245398773No Hit
CCTTTCGGGGTCACAGATCCTCTTCTGAGATGAGTTTTTG80.3067484662576687No Hit
CCTTTCGGGGGGCTGGAGAGATGGCTCAGTGGTTAAGAGC80.3067484662576687No Hit
CCTTTCGGGTCACCGATCCTCTTCTGAGATGAGTTTTTGT70.2684049079754601No Hit
CCTTTCGGGTCCCAGATCCTCTTCTGAGATGAGTTTTTGT60.23006134969325154No Hit
CCTTTCGGGTCACCGATCCTCTTCTGAAGGAGATTGGGCG60.23006134969325154No Hit
CCTTTCGGGTCACAGCTCCTCTTCTGAGATGAGTTTTTGT60.23006134969325154No Hit
CCTTTCGGGTACCTGGTTGATCCTGCCAGTAGCATATGCT60.23006134969325154No Hit
CCTTTCGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAA50.19171779141104295No Hit
CCTTTCGGGGGCACACGCATAAATACCATCTCTTGGGAAC50.19171779141104295No Hit
CCTTTCGGGGGAGAGGGAGCCTGAGAAACGGCTACCACAT50.19171779141104295No Hit
CCTTTCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGC50.19171779141104295No Hit
CCTTTCGGGGTCCGTCCGTGGAGCCAGTCCTAGCCACATT50.19171779141104295No Hit
CCTTTCGGGGGGGAGGTAGTGACGAAAAATAACAATACAG50.19171779141104295No Hit
CCTTTCGGGTCCCAGATCCTCTTCTGAAGGAGATTGGGCG50.19171779141104295No Hit
CCTTTCGGGTCACAGATCCTCTCCTGAAGGAGATTGGGCG40.15337423312883436No Hit
CCTTTCGGGGTCCCCTCCTGCCTCACAAATCCTTCAGGAA40.15337423312883436No Hit
CCTTTCGGGTCACAGACCCTCTTCTGAGATGAGTTTTTGT40.15337423312883436No Hit
CCTTTCGGGGGTGTGGGAGCAGAATGTGTTGCGAGGTTAT40.15337423312883436No Hit
CCTTTCGGGGGGAGGTAGTGACGAAAAATAACAATACAGG40.15337423312883436No Hit
CCTTTCGGGTCACAGCTCCTCTTCTGAAGGAGATTGGGCG40.15337423312883436No Hit
CCTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT40.15337423312883436No Hit
CCTTTCGGGTCACGGATCCTCTTCTGAGATGAGTTTTTGT40.15337423312883436No Hit
CCTTTCGGGTCACAGATCCTATTCTGAGATGAGTTTTTGT40.15337423312883436No Hit
CCTTTCGGGATCCAAGGAAGGCAGCAGGCGCGCAAATTAC40.15337423312883436No Hit
CCTTTCGGGGAGGCATTCAGGCAGCGAGAGCAGAGCAGCG30.11503067484662577No Hit
CCTTTCGGGACTTTGAAAAAATTAGAGTGTTCAAAGCAGG30.11503067484662577No Hit
CCTTTCGGGTCACAGATCCCCTTCTGAGATGAGTTTTTGT30.11503067484662577No Hit
CCTTTCGGGGGTTGTTTTTGGTCATTTTCTTAAAAACACA30.11503067484662577No Hit
CCTTTCGGGGGAATGAGTCCACTTTAAATCCTTTAACGAG30.11503067484662577No Hit
CCTTTCGGGTCCCCGATCCTCTTCTGAAGGAGATTGGGCG30.11503067484662577No Hit
CCTTTCGGGGGGTGACGGGGAATCAGGGTTCGATTCCGGA30.11503067484662577No Hit
CCTTTCGGGATATTAAAGTTGCTGCAGTTAAAAAGCTCGT30.11503067484662577No Hit
CCTTTCGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA30.11503067484662577No Hit
CCTTTCGGGGTCAGTGTGAATGCCTCGGCTACCCCTTTCC30.11503067484662577No Hit
CCTTTCGGGGTCACAGATCCTCTTCTGAAGGAGATTGGGC30.11503067484662577No Hit
CCTTTCGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAA30.11503067484662577No Hit
CCTTTCGGGTCACGGATCCTCTTCTGAAGGAGATTGGGCG30.11503067484662577No Hit
CCTTTCGGGAGTCAACATTCCTCTCATTTGTATATGTATG30.11503067484662577No Hit
CCTTTCGGGGATCCTCTTCTGAAGGAGATTGGGCGGATTG30.11503067484662577No Hit
CCTTTCGGGATGTTTGTTTAACTCCATATTTATTATTTTG30.11503067484662577No Hit
CCTTTCGGGTCACAGATCCTCTGAAGGAGATTGGGCGGAT30.11503067484662577No Hit
CCTTTCGGGGCCATCATATTCGTAGGAGTAAACATAACAT30.11503067484662577No Hit
CCTTTCGGGGGATCCTCTAGAGTCGACCTGCAGGCATGCA30.11503067484662577No Hit
CCTTTCGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCG30.11503067484662577No Hit
CCTTTCGGGGAACGTCTGCCCTATCAACTTTCGATGGTAG30.11503067484662577No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCGGGT404.1656676E-833.8653874
TCGGGTC404.1656676E-833.8653875
TCGGGGG301.0215979E-533.8653875
TTCGGGC501.5825208E-1033.8653834
TTCGGGA501.5825208E-1033.8653834
TCGGGGA251.5624565E-433.8653835
TCGGGCT251.5624565E-433.8653835
CCTTTCG2650.033.2264181
CTTTCGG2600.033.2141272
TTTCGGG2550.033.2013553
TTCGGGG1150.032.3929754
CGGGGTC304.5621573E-428.2211556
TCGGGGT551.8457285E-827.7080425
ATCCTCT400.002451050321.16586716
TCCTCTT400.002451050321.16586717
TGAGATG450.00431713219.18300625
Produced by FastQC (version 0.11.5)