##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1526175_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2608 Sequences flagged as poor quality 0 Sequence length 40 %GC 48 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 28.56096625766871 33.0 30.0 35.0 4.0 38.0 2 23.226610429447852 28.0 7.0 34.0 4.0 37.0 3 19.65299079754601 19.0 4.0 32.0 4.0 36.0 4 20.64378834355828 23.0 4.0 33.0 4.0 36.0 5 19.85276073619632 22.0 4.0 33.0 4.0 36.0 6 17.861579754601227 18.0 4.0 30.0 4.0 34.0 7 17.690184049079754 17.0 4.0 31.0 4.0 35.0 8 16.801380368098158 15.0 4.0 30.0 4.0 33.0 9 15.276840490797547 9.0 4.0 28.0 4.0 33.0 10 14.63803680981595 4.0 4.0 28.0 4.0 33.0 11 12.69286809815951 4.0 4.0 23.0 4.0 32.0 12 11.557898773006135 4.0 4.0 20.0 4.0 30.0 13 11.109279141104295 4.0 4.0 18.0 4.0 30.0 14 11.020705521472392 4.0 4.0 18.0 4.0 30.0 15 10.806748466257668 4.0 4.0 18.0 4.0 31.0 16 8.976610429447852 4.0 4.0 10.0 4.0 25.0 17 8.922546012269938 4.0 4.0 10.0 4.0 25.0 18 8.95935582822086 4.0 4.0 11.0 4.0 25.0 19 8.672162576687116 4.0 4.0 10.0 4.0 24.0 20 8.735429447852761 4.0 4.0 10.0 4.0 25.0 21 8.89723926380368 4.0 4.0 9.0 4.0 26.0 22 8.740030674846626 4.0 4.0 9.0 4.0 25.0 23 8.970858895705522 4.0 4.0 9.0 4.0 27.0 24 8.498849693251534 4.0 4.0 7.0 4.0 25.0 25 8.920245398773005 4.0 4.0 9.0 4.0 27.0 26 8.810966257668712 4.0 4.0 4.0 4.0 27.0 27 8.775690184049079 4.0 4.0 4.0 4.0 27.0 28 8.85659509202454 4.0 4.0 4.0 4.0 27.0 29 8.283742331288343 4.0 4.0 4.0 4.0 25.0 30 8.86196319018405 4.0 4.0 4.0 4.0 28.0 31 9.411042944785276 4.0 4.0 4.0 4.0 31.0 32 9.259969325153374 4.0 4.0 4.0 4.0 30.0 33 9.2170245398773 4.0 4.0 4.0 4.0 30.0 34 9.10084355828221 4.0 4.0 4.0 4.0 30.0 35 8.976610429447852 4.0 4.0 4.0 4.0 30.0 36 9.042561349693251 4.0 4.0 4.0 4.0 31.0 37 8.670245398773005 4.0 4.0 4.0 4.0 30.0 38 8.550230061349692 4.0 4.0 4.0 4.0 30.0 39 8.484662576687116 4.0 4.0 4.0 4.0 30.0 40 8.486579754601227 4.0 4.0 4.0 4.0 30.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 2 1 -13.228930817610067 2 2 -10.11525157232705 2 3 -11.625471698113206 2 4 -7.9251572327044 2 5 -8.202358490566038 2 6 -2.679559748427673 2 7 -5.3114779874213855 2 8 -4.76525157232704 2 9 -6.010220125786166 2 10 -0.2095911949685494 2 11 -3.001886792452831 2 12 -3.1702830188679236 2 13 -1.450943396226414 2 14 -3.43506289308176 2 15 -0.5529874213836479 2 16 0.27437106918238996 2 17 -1.3805031446540852 2 18 -1.3455974842767295 2 19 1.1018867924528308 2 20 -2.8797169811320744 2 21 -0.37389937106918225 2 22 -3.576415094339623 2 23 -0.09135220125786248 2 24 -2.7124213836477997 2 25 -0.7199685534591183 2 26 0.315880503144653 2 27 -0.43836477987421496 2 28 0.8731132075471688 2 29 -1.8987421383647805 2 30 0.21210691823899452 2 31 1.034591194968554 2 32 -0.5869496855345933 2 33 -1.2797169811320783 2 34 -0.9026729559748432 2 35 -2.458962264150945 2 36 0.9048742138364787 2 37 1.587735849056604 2 38 -3.9617924528301884 2 39 -3.842138364779875 2 40 -3.60927672955975 3 1 -2.8289308176100683 3 2 1.2847484276729517 3 3 -10.625471698113206 3 4 -4.1251572327043995 3 5 -7.202358490566038 3 6 -2.2795597484276726 3 7 -5.111477987421386 3 8 1.4347484276729592 3 9 3.989779874213834 3 10 0.19040880503145097 3 11 3.5981132075471685 3 12 3.0297169811320757 3 13 4.549056603773586 3 14 2.9649371069182404 3 15 2.0470125786163536 3 16 7.27437106918239 3 17 7.019496855345915 3 18 8.254402515723271 3 19 5.501886792452831 3 20 7.720283018867926 3 21 6.826100628930817 3 22 5.623584905660378 3 23 10.108647798742139 3 24 6.4875786163522005 3 25 5.880031446540881 3 26 7.915880503144653 3 27 11.961635220125785 3 28 11.273113207547167 3 29 1.70125786163522 3 30 7.812106918238994 3 31 5.834591194968553 3 32 12.013050314465408 3 33 8.92028301886792 3 34 5.697327044025156 3 35 7.141037735849055 3 36 11.304874213836479 3 37 8.987735849056605 3 38 13.038207547169812 3 39 11.757861635220127 3 40 12.190723270440252 4 1 8.971069182389932 4 2 14.351415094339615 4 3 13.70786163522013 4 4 11.541509433962268 4 5 15.797641509433962 4 6 15.587106918238995 4 7 15.288522012578614 4 8 12.901415094339628 4 9 13.789779874213835 4 10 2.0570754716981163 4 11 10.598113207547168 4 12 11.496383647798744 4 13 15.949056603773586 4 14 11.498270440251572 4 15 13.247012578616353 4 16 9.007704402515722 4 17 5.286163522012581 4 18 11.45440251572327 4 19 7.101886792452831 4 20 11.253616352201258 4 21 10.95943396226415 4 22 14.823584905660377 4 23 16.308647798742136 4 24 9.420911949685532 4 25 9.880031446540881 4 26 18.915880503144653 4 27 15.294968553459118 4 28 17.2064465408805 4 29 11.967924528301888 4 30 16.47877358490566 4 31 15.234591194968553 4 32 21.54638364779874 4 33 17.52028301886792 4 34 17.83066037735849 4 35 23.674371069182392 4 36 16.83820754716981 4 37 29.85440251572327 4 38 26.03820754716981 4 39 26.157861635220126 4 40 29.39072327044025 5 1 -6.695597484276735 5 2 -18.31525157232705 5 3 -14.625471698113206 5 4 -16.1251572327044 5 5 -15.202358490566038 5 6 -12.079559748427673 5 7 -12.711477987421386 5 8 -11.76525157232704 5 9 -10.210220125786165 5 10 -9.60959119496855 5 11 -7.4018867924528315 5 12 -7.170283018867924 5 13 -6.050943396226414 5 14 -6.83506289308176 5 15 -5.752987421383647 5 16 -4.32562893081761 5 17 -4.380503144654085 5 18 -4.54559748427673 5 19 -3.898113207547169 5 20 -4.079716981132075 5 21 -4.373899371069182 5 22 -4.176415094339623 5 23 -4.691352201257862 5 24 -3.9124213836478 5 25 -4.119968553459119 5 26 -4.084119496855347 5 27 -4.038364779874215 5 28 -4.126886792452831 5 29 -3.6987421383647803 5 30 -4.187893081761006 5 31 -4.765408805031447 5 32 -4.7869496855345925 5 33 -4.4797169811320785 5 34 -4.502672955974843 5 35 -4.658962264150945 5 36 -4.495125786163522 5 37 -3.8122641509433963 5 38 -3.9617924528301884 5 39 -3.842138364779875 5 40 -3.60927672955975 6 1 7.77106918238993 6 2 10.884748427672953 6 3 16.17452830188679 6 4 11.474842767295602 6 5 15.997641509433965 6 6 3.120440251572326 6 7 7.8885220125786155 6 8 6.6347484276729585 6 9 6.389779874213836 6 10 6.990408805031452 6 11 9.19811320754717 6 12 7.229716981132075 6 13 0.549056603773586 6 14 6.96493710691824 6 15 14.847012578616354 6 16 9.274371069182392 6 17 5.619496855345915 6 18 9.854402515723269 6 19 9.901886792452832 6 20 4.720283018867926 6 21 9.22610062893082 6 22 9.823584905660377 6 23 10.108647798742139 6 24 11.487578616352199 6 25 11.08003144654088 6 26 9.915880503144653 6 27 17.161635220125785 6 28 15.47311320754717 6 29 13.10125786163522 6 30 13.212106918238993 6 31 18.834591194968553 6 32 14.013050314465408 6 33 13.92028301886792 6 34 13.097327044025159 6 35 12.341037735849055 6 36 11.304874213836479 6 37 1.7877358490566033 6 38 6.238207547169811 6 39 -0.042138364779875204 6 40 2.190723270440251 7 1 -17.778930817610068 7 2 -12.06525157232705 7 3 -11.875471698113206 7 4 -10.8751572327044 7 5 -11.702358490566038 7 6 -6.329559748427673 7 7 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6.5981132075471685 9 12 2.8297169811320764 9 13 9.282389937106919 9 14 4.831603773584906 9 15 9.91367924528302 9 16 12.007704402515722 9 17 9.952830188679247 9 18 8.787735849056602 9 19 11.435220125786163 9 20 7.5869496855345915 9 21 9.626100628930818 9 22 5.156918238993711 9 23 1.975314465408804 9 24 9.420911949685532 9 25 3.2133647798742153 9 26 5.249213836477987 9 27 10.628301886792453 9 28 9.206446540880501 9 29 10.30125786163522 9 30 7.812106918238994 9 31 9.234591194968553 9 32 9.213050314465407 9 33 14.853616352201254 9 34 8.163993710691825 9 35 7.341037735849055 9 36 8.83820754716981 9 37 12.521069182389937 9 38 8.038207547169812 9 39 10.491194968553458 9 40 6.39072327044025 11 1 6.4710691823899325 11 2 10.18474842767295 11 3 4.624528301886794 11 4 10.1248427672956 11 5 9.797641509433962 11 6 15.920440251572327 11 7 16.038522012578614 11 8 15.48474842767296 11 9 16.289779874213835 11 10 8.14040880503145 11 11 12.848113207547168 11 12 12.829716981132076 11 13 2.6990566037735864 11 14 6.41493710691824 11 15 6.497012578616353 11 16 6.17437106918239 11 17 8.619496855345915 11 18 8.45440251572327 11 19 6.851886792452831 11 20 5.420283018867925 11 21 5.126100628930818 11 22 3.8235849056603772 11 23 3.308647798742138 11 24 1.0875786163522 11 25 3.6300314465408814 11 26 1.1658805031446526 11 27 -0.5383647798742146 11 28 6.123113207547169 11 29 9.80125786163522 11 30 3.062106918238994 11 31 3.2345911949685533 11 32 4.9630503144654075 11 33 4.7702830188679215 11 34 6.747327044025157 11 35 6.591037735849055 11 36 5.504874213836478 11 37 0.4377358490566037 11 38 10.538207547169812 11 39 6.407861635220125 11 40 8.390723270440251 12 1 -2.228930817610067 12 2 -2.7152515723270483 12 3 -9.625471698113206 12 4 -6.325157232704399 12 5 -0.6023584905660364 12 6 -0.679559748427673 12 7 2.8885220125786155 12 8 2.23474842767296 12 9 2.5897798742138356 12 10 0.19040880503145097 12 11 7.5981132075471685 12 12 12.429716981132078 12 13 9.949056603773586 12 14 11.16493710691824 12 15 8.647012578616351 12 16 9.074371069182389 12 17 5.4194968553459155 12 18 0.4544025157232703 12 19 -2.498113207547169 12 20 1.7202830188679261 12 21 0.826100628930817 12 22 -1.5764150943396231 12 23 -2.491352201257862 12 24 -0.11242138364780008 12 25 -2.519968553459119 12 26 1.315880503144653 12 27 1.3616352201257858 12 28 0.27311320754716917 12 29 -2.49874213836478 12 30 -1.7878930817610055 12 31 0.8345911949685529 12 32 -1.7869496855345925 12 33 -1.8797169811320789 12 34 -0.1026729559748425 12 35 -2.458962264150945 12 36 -0.6951257861635218 12 37 -0.012264150943396501 12 38 0.8382075471698123 12 39 1.5578616352201253 12 40 -0.6092767295597499 13 1 7.2210691823899325 13 2 10.68474842767295 13 3 15.374528301886794 13 4 11.1248427672956 13 5 15.797641509433962 13 6 8.670440251572327 13 7 15.788522012578614 13 8 8.48474842767296 13 9 6.289779874213835 13 10 10.64040880503145 13 11 1.8481132075471685 13 12 6.329716981132076 13 13 3.9490566037735864 13 14 -2.3350628930817603 13 15 6.997012578616353 13 16 -2.0756289308176097 13 17 2.619496855345915 13 18 0.20440251572327028 13 19 0.8518867924528308 13 20 1.4202830188679254 13 21 2.1261006289308177 13 22 3.5735849056603772 13 23 3.058647798742138 13 24 0.8375786163522001 13 25 2.3800314465408814 13 26 2.6658805031446526 13 27 -0.7883647798742146 13 28 1.8731132075471688 13 29 -1.4487421383647803 13 30 2.562106918238994 13 31 2.4845911949685533 13 32 2.4630503144654075 13 33 2.7702830188679215 13 34 2.747327044025157 13 35 1.8410377358490546 13 36 -0.49512578616352165 13 37 0.18773584905660368 13 38 -0.7117924528301884 13 39 3.407861635220125 13 40 3.14072327044025 14 1 4.9710691823899325 14 2 7.0180817610062824 14 3 5.707861635220127 14 4 6.541509433962268 14 5 -2.8690251572327057 14 6 13.920440251572327 14 7 13.955188679245282 14 8 18.23474842767296 14 9 17.456446540880503 14 10 10.723742138364782 14 11 -3.0685534591194976 14 12 -2.5036163522012576 14 13 -2.7176100628930806 14 14 -5.168396226415093 14 15 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21.674371069182392 16 17 19.119496855345915 16 18 15.45440251572327 16 19 11.60188679245283 16 20 17.920283018867927 16 21 10.626100628930818 16 22 15.823584905660377 16 23 12.808647798742138 16 24 17.0875786163522 16 25 9.880031446540881 16 26 12.415880503144653 16 27 17.961635220125785 16 28 11.873113207547169 16 29 19.30125786163522 16 30 13.312106918238994 16 31 17.73459119496855 16 32 22.213050314465406 16 33 15.520283018867921 16 34 22.497327044025155 16 35 19.341037735849056 16 36 26.00487421383648 16 37 9.187735849056605 16 38 12.038207547169812 16 39 12.157861635220126 16 40 7.89072327044025 17 1 -0.5289308176100675 17 2 -10.31525157232705 17 3 -3.6254716981132056 17 4 -8.1251572327044 17 5 -7.202358490566038 17 6 -4.079559748427673 17 7 2.288522012578614 17 8 2.23474842767296 17 9 0.7897798742138349 17 10 13.39040880503145 17 11 15.098113207547168 17 12 13.329716981132076 17 13 17.449056603773585 17 14 19.664937106918238 17 15 17.247012578616353 17 16 6.67437106918239 17 17 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0.0875786163522001 111 25 0.5466981132075475 111 26 0.24921383647798656 111 27 2.2949685534591193 111 28 1.8731132075471688 111 29 0.9679245283018858 111 30 1.145440251572328 111 31 1.5679245283018872 111 32 -1.7869496855345925 111 33 -0.14638364779874458 111 34 -0.5026729559748428 111 35 2.6743710691823885 111 36 1.8382075471698123 111 37 5.85440251572327 111 38 5.038207547169812 111 39 2.491194968553459 111 40 3.724056603773584 112 1 6.9710691823899325 112 2 9.018081761006282 112 3 8.041194968553462 112 4 7.208176100628933 112 5 10.797641509433962 112 6 18.58710691823899 112 7 14.955188679245282 112 8 12.23474842767296 112 9 14.456446540880503 112 10 0.7237421383647842 112 11 5.5981132075471685 112 12 7.496383647798744 112 13 12.282389937106919 112 14 2.4982704402515736 112 15 3.5803459119496868 112 16 5.341037735849056 112 17 3.286163522012581 112 18 4.121069182389936 112 19 5.768553459119497 112 20 3.5869496855345915 112 21 3.6261006289308177 112 22 2.8235849056603772 112 23 0.975314465408804 112 24 1.7542452830188662 112 25 1.5466981132075475 112 26 3.2492138364779866 112 27 3.9616352201257854 112 28 4.539779874213835 112 29 2.3012578616352197 112 30 3.812106918238994 112 31 2.567924528301887 112 32 5.546383647798741 112 33 5.853616352201255 112 34 4.163993710691823 112 35 4.007704402515721 112 36 4.838207547169812 112 37 7.521069182389938 112 38 6.704874213836478 112 39 6.491194968553459 112 40 7.724056603773584 113 1 6.9710691823899325 113 2 12.68474842767295 113 3 8.874528301886794 113 4 13.8748427672956 113 5 0.29764150943396217 113 6 11.920440251572327 113 7 12.788522012578614 113 8 0.7347484276729599 113 9 3.789779874213835 113 10 -9.60959119496855 113 11 -7.4018867924528315 113 12 -7.170283018867924 113 13 -6.050943396226414 113 14 -6.83506289308176 113 15 -5.752987421383647 113 16 -4.32562893081761 113 17 -4.380503144654085 113 18 -4.54559748427673 113 19 -3.898113207547169 113 20 -4.079716981132075 113 21 -4.373899371069182 113 22 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-3.898113207547169 115 20 -4.079716981132075 115 21 -4.373899371069182 115 22 -4.176415094339623 115 23 -4.691352201257862 115 24 -3.9124213836478 115 25 -4.119968553459119 115 26 -4.084119496855347 115 27 -4.038364779874215 115 28 -4.126886792452831 115 29 -3.6987421383647803 115 30 -4.187893081761006 115 31 -4.765408805031447 115 32 -4.7869496855345925 115 33 -4.4797169811320785 115 34 -4.502672955974843 115 35 -4.658962264150945 115 36 -4.495125786163522 115 37 -3.8122641509433963 115 38 -3.9617924528301884 115 39 -3.842138364779875 115 40 -3.60927672955975 116 1 -24.028930817610068 116 2 -18.31525157232705 116 3 -14.625471698113206 116 4 -16.1251572327044 116 5 -15.202358490566038 116 6 -12.079559748427673 116 7 -12.711477987421386 116 8 -11.76525157232704 116 9 -10.210220125786165 116 10 -9.60959119496855 116 11 -7.4018867924528315 116 12 -7.170283018867924 116 13 -6.050943396226414 116 14 -6.83506289308176 116 15 -5.752987421383647 116 16 -4.32562893081761 116 17 -4.380503144654085 116 18 -4.54559748427673 116 19 -3.898113207547169 116 20 -4.079716981132075 116 21 -4.373899371069182 116 22 -4.176415094339623 116 23 -4.691352201257862 116 24 -3.9124213836478 116 25 -4.119968553459119 116 26 -4.084119496855347 116 27 -4.038364779874215 116 28 -4.126886792452831 116 29 -3.6987421383647803 116 30 -4.187893081761006 116 31 -4.765408805031447 116 32 -4.7869496855345925 116 33 -4.4797169811320785 116 34 -4.502672955974843 116 35 -4.658962264150945 116 36 -4.495125786163522 116 37 -3.8122641509433963 116 38 -3.9617924528301884 116 39 -3.842138364779875 116 40 -3.60927672955975 117 1 6.4710691823899325 117 2 12.18474842767295 117 3 8.874528301886794 117 4 14.3748427672956 117 5 10.297641509433962 117 6 10.420440251572327 117 7 -4.211477987421386 117 8 18.23474842767296 117 9 19.289779874213835 117 10 3.3904088050314503 117 11 -7.4018867924528315 117 12 -7.170283018867924 117 13 -6.050943396226414 117 14 -6.83506289308176 117 15 -5.752987421383647 117 16 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13 -6.050943396226414 119 14 -6.83506289308176 119 15 -5.752987421383647 119 16 -4.32562893081761 119 17 -4.380503144654085 119 18 -4.54559748427673 119 19 -3.898113207547169 119 20 -4.079716981132075 119 21 -4.373899371069182 119 22 -4.176415094339623 119 23 -4.691352201257862 119 24 -3.9124213836478 119 25 -4.119968553459119 119 26 -4.084119496855347 119 27 -4.038364779874215 119 28 -4.126886792452831 119 29 -3.6987421383647803 119 30 -4.187893081761006 119 31 -4.765408805031447 119 32 -4.7869496855345925 119 33 -4.4797169811320785 119 34 -4.502672955974843 119 35 -4.658962264150945 119 36 -4.495125786163522 119 37 -3.8122641509433963 119 38 -3.9617924528301884 119 39 -3.842138364779875 119 40 -3.60927672955975 120 1 4.9710691823899325 120 2 -5.31525157232705 120 3 -4.125471698113206 120 4 -1.6251572327043995 120 5 -15.202358490566038 120 6 -12.079559748427673 120 7 -12.711477987421386 120 8 -11.76525157232704 120 9 -10.210220125786165 120 10 -9.60959119496855 120 11 -7.4018867924528315 120 12 -7.170283018867924 120 13 -6.050943396226414 120 14 -6.83506289308176 120 15 -5.752987421383647 120 16 -4.32562893081761 120 17 -4.380503144654085 120 18 -4.54559748427673 120 19 -3.898113207547169 120 20 -4.079716981132075 120 21 -4.373899371069182 120 22 -4.176415094339623 120 23 -4.691352201257862 120 24 -3.9124213836478 120 25 -4.119968553459119 120 26 -4.084119496855347 120 27 -4.038364779874215 120 28 -4.126886792452831 120 29 -3.6987421383647803 120 30 -4.187893081761006 120 31 -4.765408805031447 120 32 -4.7869496855345925 120 33 -4.4797169811320785 120 34 -4.502672955974843 120 35 -4.658962264150945 120 36 -4.495125786163522 120 37 -3.8122641509433963 120 38 -3.9617924528301884 120 39 -3.842138364779875 120 40 -3.60927672955975 >>END_MODULE >>Per sequence quality scores fail #Quality Count 4 639.0 5 115.0 6 114.0 7 182.0 8 162.0 9 170.0 10 164.0 11 133.0 12 82.0 13 69.0 14 47.0 15 34.0 16 36.0 17 29.0 18 28.0 19 43.0 20 58.0 21 81.0 22 78.0 23 86.0 24 75.0 25 61.0 26 39.0 27 42.0 28 20.0 29 10.0 30 4.0 31 3.0 32 2.0 33 1.0 34 1.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 0.0 0.0 0.0 100.0 2 0.0 0.0 0.0 100.0 3 0.0 0.0 100.0 0.0 4 0.0 0.0 100.0 0.0 5 0.0 0.0 100.0 0.0 6 0.0 0.0 0.0 100.0 7 98.31288343558282 0.49846625766871167 0.651840490797546 0.5368098159509203 8 97.88948580199539 0.920951650038373 0.7290867229470452 0.4604758250191865 9 96.23655913978494 0.7296466973886329 2.1889400921658986 0.8448540706605223 10 38.88675623800384 25.911708253358924 20.72936660268714 14.472168905950095 11 24.118098159509202 22.04754601226994 24.616564417177912 29.217791411042942 12 26.30368098159509 30.13803680981595 22.04754601226994 21.51073619631902 13 20.62883435582822 20.89723926380368 24.654907975460123 33.81901840490797 14 19.32515337423313 37.423312883435585 23.92638036809816 19.32515337423313 15 36.924846625766875 25.61349693251534 22.661042944785276 14.800613496932517 16 26.11196319018405 37.308282208588956 22.200920245398773 14.378834355828221 17 22.14121258633922 24.405218726016884 37.6438986953185 15.809669992325404 18 21.970858895705522 23.044478527607364 24.884969325153374 30.099693251533743 19 17.613200306983885 25.057559478127402 27.59017651573292 29.739063699155793 20 20.43711656441718 23.696319018404907 38.305214723926376 17.561349693251536 21 18.021472392638035 21.70245398773006 28.642638036809814 31.633435582822084 22 16.180981595092025 19.670245398773005 41.83282208588957 22.3159509202454 23 16.449386503067483 26.91717791411043 40.68251533742331 15.950920245398773 24 15.567484662576685 27.108895705521473 26.533742331288344 30.7898773006135 25 19.961612284069098 25.067178502879077 36.046065259117086 18.925143953934743 26 34.89623366641045 29.323597232897768 20.67640276710223 15.103766333589547 27 19.207082371054657 38.52963818321786 21.709006928406467 20.554272517321014 28 25.23006134969325 34.93098159509203 23.466257668711656 16.37269938650307 29 28.33525567089581 31.410995770857365 23.18339100346021 17.070357554786618 30 23.773006134969325 27.415644171779142 33.013803680981596 15.797546012269938 31 27.530674846625768 32.515337423312886 23.773006134969325 16.180981595092025 32 25.941583397386626 34.47348193697156 22.713297463489624 16.87163720215219 33 28.5659509202454 30.904907975460123 23.006134969325153 17.523006134969325 34 18.82668711656442 26.035276073619634 38.65030674846626 16.487730061349694 35 17.101226993865033 28.144171779141104 38.95705521472393 15.797546012269938 36 23.357664233576642 28.8897426046869 31.57894736842105 16.173645793315405 37 23.96472392638037 26.45705521472393 33.512269938650306 16.0659509202454 38 24.348159509202453 27.799079754601223 31.556748466257666 16.29601226993865 39 25.49846625766871 28.067484662576685 24.233128834355828 22.200920245398773 40 23.466257668711656 26.648773006134967 32.36196319018405 17.523006134969325 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 3.5 7 7.0 8 7.0 9 5.5 10 4.0 11 3.0 12 2.0 13 2.0 14 2.5 15 3.0 16 9.0 17 15.0 18 15.0 19 15.5 20 16.0 21 23.0 22 30.0 23 30.0 24 33.0 25 36.0 26 33.5 27 31.0 28 31.0 29 39.5 30 48.0 31 53.5 32 59.0 33 59.0 34 63.5 35 68.0 36 91.0 37 114.0 38 114.0 39 118.0 40 122.0 41 158.0 42 194.0 43 194.0 44 226.5 45 259.0 46 252.5 47 246.0 48 246.0 49 225.5 50 205.0 51 217.5 52 230.0 53 230.0 54 258.0 55 286.0 56 240.0 57 194.0 58 194.0 59 180.5 60 167.0 61 156.0 62 145.0 63 145.0 64 105.0 65 65.0 66 47.5 67 30.0 68 30.0 69 23.0 70 16.0 71 12.0 72 8.0 73 8.0 74 6.5 75 5.0 76 3.5 77 2.0 78 2.0 79 1.0 80 0.0 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.5 90 1.0 91 0.5 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.07668711656441718 9 0.15337423312883436 10 0.11503067484662577 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.07668711656441718 18 0.0 19 0.07668711656441718 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.11503067484662577 26 0.23006134969325154 27 0.3834355828220859 28 0.0 29 0.2684049079754601 30 0.0 31 0.0 32 0.23006134969325154 33 0.0 34 0.0 35 0.0 36 0.19171779141104295 37 0.0 38 0.0 39 0.0 40 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 40 2608.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 84.3558282208589 #Duplication Level Percentage of deduplicated Percentage of total 1 92.77272727272728 78.25920245398773 2 4.909090909090909 8.282208588957054 3 0.9545454545454546 2.415644171779141 4 0.45454545454545453 1.5337423312883436 5 0.3181818181818182 1.3420245398773007 6 0.18181818181818182 0.9202453987730062 7 0.045454545454545456 0.2684049079754601 8 0.09090909090909091 0.6134969325153374 9 0.13636363636363638 1.0352760736196318 >10 0.09090909090909091 2.415644171779141 >50 0.045454545454545456 2.9141104294478524 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source CCTTTCGGGTCACAGATCCTCTTCTGAGATGAGTTTTTGT 76 2.9141104294478524 No Hit CCTTTCGGGTCACAGATCCTCTTCTGAAGGAGATTGGGCG 46 1.763803680981595 No Hit CCTTTCGGGTCAAAGATCCTCTTCTGAGATGAGTTTTTGT 17 0.651840490797546 No Hit CCTTTCGGGTCAAAGATCCTCTTCTGAAGGAGATTGGGCG 9 0.3450920245398773 No Hit CCTTTCGGTCACAGATCCTCTTCTGAGATGAGTTTTTGTT 9 0.3450920245398773 No Hit CCTTTCGGGGTCTGTCCGTCCGTGGAGCCAGTCCTAGCCA 9 0.3450920245398773 No Hit CCTTTCGGGGTCACAGATCCTCTTCTGAGATGAGTTTTTG 8 0.3067484662576687 No Hit CCTTTCGGGGGGCTGGAGAGATGGCTCAGTGGTTAAGAGC 8 0.3067484662576687 No Hit CCTTTCGGGTCACCGATCCTCTTCTGAGATGAGTTTTTGT 7 0.2684049079754601 No Hit CCTTTCGGGTCCCAGATCCTCTTCTGAGATGAGTTTTTGT 6 0.23006134969325154 No Hit CCTTTCGGGTCACCGATCCTCTTCTGAAGGAGATTGGGCG 6 0.23006134969325154 No Hit CCTTTCGGGTCACAGCTCCTCTTCTGAGATGAGTTTTTGT 6 0.23006134969325154 No Hit CCTTTCGGGTACCTGGTTGATCCTGCCAGTAGCATATGCT 6 0.23006134969325154 No Hit CCTTTCGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAA 5 0.19171779141104295 No Hit CCTTTCGGGGGCACACGCATAAATACCATCTCTTGGGAAC 5 0.19171779141104295 No Hit CCTTTCGGGGGAGAGGGAGCCTGAGAAACGGCTACCACAT 5 0.19171779141104295 No Hit CCTTTCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGC 5 0.19171779141104295 No Hit CCTTTCGGGGTCCGTCCGTGGAGCCAGTCCTAGCCACATT 5 0.19171779141104295 No Hit CCTTTCGGGGGGGAGGTAGTGACGAAAAATAACAATACAG 5 0.19171779141104295 No Hit CCTTTCGGGTCCCAGATCCTCTTCTGAAGGAGATTGGGCG 5 0.19171779141104295 No Hit CCTTTCGGGTCACAGATCCTCTCCTGAAGGAGATTGGGCG 4 0.15337423312883436 No Hit CCTTTCGGGGTCCCCTCCTGCCTCACAAATCCTTCAGGAA 4 0.15337423312883436 No Hit CCTTTCGGGTCACAGACCCTCTTCTGAGATGAGTTTTTGT 4 0.15337423312883436 No Hit CCTTTCGGGGGTGTGGGAGCAGAATGTGTTGCGAGGTTAT 4 0.15337423312883436 No Hit CCTTTCGGGGGGAGGTAGTGACGAAAAATAACAATACAGG 4 0.15337423312883436 No Hit CCTTTCGGGTCACAGCTCCTCTTCTGAAGGAGATTGGGCG 4 0.15337423312883436 No Hit CCTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4 0.15337423312883436 No Hit CCTTTCGGGTCACGGATCCTCTTCTGAGATGAGTTTTTGT 4 0.15337423312883436 No Hit CCTTTCGGGTCACAGATCCTATTCTGAGATGAGTTTTTGT 4 0.15337423312883436 No Hit CCTTTCGGGATCCAAGGAAGGCAGCAGGCGCGCAAATTAC 4 0.15337423312883436 No Hit CCTTTCGGGGAGGCATTCAGGCAGCGAGAGCAGAGCAGCG 3 0.11503067484662577 No Hit CCTTTCGGGACTTTGAAAAAATTAGAGTGTTCAAAGCAGG 3 0.11503067484662577 No Hit CCTTTCGGGTCACAGATCCCCTTCTGAGATGAGTTTTTGT 3 0.11503067484662577 No Hit CCTTTCGGGGGTTGTTTTTGGTCATTTTCTTAAAAACACA 3 0.11503067484662577 No Hit CCTTTCGGGGGAATGAGTCCACTTTAAATCCTTTAACGAG 3 0.11503067484662577 No Hit CCTTTCGGGTCCCCGATCCTCTTCTGAAGGAGATTGGGCG 3 0.11503067484662577 No Hit CCTTTCGGGGGGTGACGGGGAATCAGGGTTCGATTCCGGA 3 0.11503067484662577 No Hit CCTTTCGGGATATTAAAGTTGCTGCAGTTAAAAAGCTCGT 3 0.11503067484662577 No Hit CCTTTCGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 3 0.11503067484662577 No Hit CCTTTCGGGGTCAGTGTGAATGCCTCGGCTACCCCTTTCC 3 0.11503067484662577 No Hit CCTTTCGGGGTCACAGATCCTCTTCTGAAGGAGATTGGGC 3 0.11503067484662577 No Hit CCTTTCGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 3 0.11503067484662577 No Hit CCTTTCGGGTCACGGATCCTCTTCTGAAGGAGATTGGGCG 3 0.11503067484662577 No Hit CCTTTCGGGAGTCAACATTCCTCTCATTTGTATATGTATG 3 0.11503067484662577 No Hit CCTTTCGGGGATCCTCTTCTGAAGGAGATTGGGCGGATTG 3 0.11503067484662577 No Hit CCTTTCGGGATGTTTGTTTAACTCCATATTTATTATTTTG 3 0.11503067484662577 No Hit CCTTTCGGGTCACAGATCCTCTGAAGGAGATTGGGCGGAT 3 0.11503067484662577 No Hit CCTTTCGGGGCCATCATATTCGTAGGAGTAAACATAACAT 3 0.11503067484662577 No Hit CCTTTCGGGGGATCCTCTAGAGTCGACCTGCAGGCATGCA 3 0.11503067484662577 No Hit CCTTTCGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCG 3 0.11503067484662577 No Hit CCTTTCGGGGAACGTCTGCCCTATCAACTTTCGATGGTAG 3 0.11503067484662577 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0 0.0 0.0 0.0 0.0 11 0.0 0.0 0.0 0.0 0.0 12 0.0 0.0 0.0 0.0 0.0 13 0.0 0.0 0.0 0.0 0.0 14 0.0 0.0 0.0 0.0 0.0 15 0.0 0.0 0.0 0.0 0.0 16 0.0 0.0 0.0 0.0 0.0 17 0.0 0.0 0.0 0.0 0.0 18 0.0 0.0 0.0 0.0 0.0 19 0.0 0.0 0.0 0.0 0.0 20 0.0 0.0 0.0 0.0 0.0 21 0.0 0.0 0.0 0.0 0.0 22 0.0 0.0 0.0 0.0 0.0 23 0.0 0.0 0.0 0.0 0.0 24 0.0 0.0 0.0 0.0 0.0 25 0.0 0.0 0.0 0.0 0.0 26 0.0 0.0 0.0 0.0 0.0 27 0.0 0.0 0.0 0.0 0.0 28 0.0 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TTCGGGT 40 4.1656676E-8 33.865387 4 TCGGGTC 40 4.1656676E-8 33.865387 5 TCGGGGG 30 1.0215979E-5 33.865387 5 TTCGGGC 50 1.5825208E-10 33.865383 4 TTCGGGA 50 1.5825208E-10 33.865383 4 TCGGGGA 25 1.5624565E-4 33.865383 5 TCGGGCT 25 1.5624565E-4 33.865383 5 CCTTTCG 265 0.0 33.226418 1 CTTTCGG 260 0.0 33.214127 2 TTTCGGG 255 0.0 33.201355 3 TTCGGGG 115 0.0 32.392975 4 CGGGGTC 30 4.5621573E-4 28.221155 6 TCGGGGT 55 1.8457285E-8 27.708042 5 ATCCTCT 40 0.0024510503 21.165867 16 TCCTCTT 40 0.0024510503 21.165867 17 TGAGATG 45 0.004317132 19.183006 25 >>END_MODULE