FastQCFastQC Report
Fri 17 Jun 2016
SRR1526174_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526174_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23753
Sequences flagged as poor quality0
Sequence length56
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACAAGGGTCACAGATCCTCTTCTGAGATGAGTTTTTGTTCAATCCGCCCAATCT5872.4712667873531764No Hit
CAACAAGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAG4381.8439776028291162No Hit
CAACAAGGGTCACAGATCCTCTTCTGAAGGAGATTGGGCGGATTGAACAAAAACTC3201.3471982486422767No Hit
CAACAAGGGTCACAGATCCTCTTCTGAGATGAGTTTTTGTCCAATCCGCCCAATCT1550.6525491516861028No Hit
CAACAAGGGTCACAGATCCTCTTCTGAAGGAGATTGGGCGCATTGAACAAAAACTC1420.5978192228350103No Hit
CAACAAGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTCGTCTCAAAGATTAAG1090.45888940344377555No Hit
CAACAAGGGGGGCGCTGACCCCCCTTCCCGGGGGGGGATGCGTGCATTTATCAGAT710.29890961141750516No Hit
CAACAAGGGTCACAGATCCTCTTCTGAGATGAGTTTTTGTTCAATCCGCCCAAAAA610.256809666147434No Hit
CAACAAGGGTACCTGGTTGATCCTGCCAGTAGCATATGCCTGTCTCAAAGATTAAG580.24417968256641265No Hit
CAACAAGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCCAAGATTAAG580.24417968256641265No Hit
CAACAAGGGAACTGTGGTAATTCTAGAGCTAATACATGCCGACGGGCGCTGACCCC500.21049972635035574No Hit
CAACAAGGGTCACAGATCCTCTTCTGAAGGAGATTGGGCCGATTGAACAAAAACTC480.20207973729634154No Hit
CAACAAGGGATGGTTCCTTTGGTCGCTCGCTCCTCTCCTACTTGGATAACTGTGGT470.1978697427693344No Hit
CAACAAGGGGGTTCCTTTGGTCGCTCGCTCCTCTCCTACTTGGATAACTGTGGTAA400.16839978108028458No Hit
CAACAAGGGTCACAGATCCTCTTCTGAGATGAGTTTTTGCTCAATCCGCCCAATCT390.16418978655327748No Hit
CAACAAGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCCACGATTAAG360.15155980297225613No Hit
CAACAAGGGAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGTC360.15155980297225613No Hit
CAACAAGGGGAACGTCTGCCCTATCAACTTTCGATGGTAGTCGCCGTGCCTACCAT350.14734980844524903No Hit
CAACAAGGGGGTGAGCTCCCTCCCGGCTCCGGCCGGGGGTCGGGCGCCGGCGGCTT350.14734980844524903No Hit
CAACAAGGGCTCCTACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCGAC330.1389298193912348No Hit
CAACAAGGGAGTTATGGTTCCTTTGGTCGCTCGCTCCTCTCCTACTTGGATAACTG320.13471982486422768No Hit
CAACAAGGGATCAGATCAAAACCAACCCGGTGAGCTCCCTCCCGGCTCCGGCCGGG320.13471982486422768No Hit
CAACAAGGGTCACAGATCCTCTTCTGAAGGAGATTGGGCGGATTGACCAAAAACTC320.13471982486422768No Hit
CAACAAGGGTACCTGGTTGATCCTGCCAGTAGCATATGCCGTCTTCTGCTTGGATC300.12629983581021345No Hit
CAACAAGGTCACAGATCCTCTTCTGAGATGAGTTTTTGTTCAATCCGCCCAATCTC290.12208984128320632No Hit
CAACAAGGGTCACAGATCCTCTGAAGGAGATTGGGCGGATTGAACAAAAACTCATC280.11787984675619921No Hit
CAACAAGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA280.11787984675619921No Hit
CAACAAGGGTCACAGATCCTCTCCTGAAGGAGATTGGGCGGATTGAACAAAAACTC260.10945985770218498No Hit
CAACAAGGGAACTGTGGTAATTCTAGAGCTAATACATGCCCACGGGCGCTGACCCC250.10524986317517787No Hit
CAACAAGGGGTCACAGATCCTCTTCTGAGATGAGTTTTTGTTCAATCCGCCCAATC240.10103986864817077No Hit
CAACAAGGGGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCCATTCGAACGTC240.10103986864817077No Hit
CAACAAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC240.10103986864817077No Hit
CAACAAGGGGTGGCGGCGACGACCCATTCGAACGTCTGCCCTATCAACTTTCGATG240.10103986864817077No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTGTCCA203.8341625E-450.68240437
TTGTTCC203.8341625E-450.68240437
GTTCCCT203.8341625E-450.68240439
TGCCTGT150.00789385450.68240437
TCCAATC203.8341625E-450.68240440
CTGTCTC150.00789385450.68240440
GCCTGTC150.00789385450.68240438
GGCGCAT650.050.682437
ATGCCTG150.00796124350.5738836
TTTGCTC150.00796124350.5738836
TGTCTCC203.875112E-450.57387541
TTTGTCC203.875112E-450.57387536
TATGCCT150.0080290650.46581335
ATATGCC203.9580528E-450.35820834
TCCCAAT150.00823509250.14437548
CCCTCCG204.0423987E-450.14437542
GGGCCCT150.00823509250.14437533
TCCAAGA204.0851036E-450.03813645
CTCTGAA204.1281662E-449.9323519
CCTATCA150.00837461449.9323520