##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1526174_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	23753
Sequences flagged as poor quality	0
Sequence length	56
%GC	49
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	36.67498842251505	39.0	38.0	39.0	33.0	40.0
2	34.790721172062476	38.0	35.0	39.0	21.0	40.0
3	35.294531217109416	38.0	36.0	39.0	27.0	40.0
4	34.88792994569107	38.0	35.0	39.0	26.0	40.0
5	34.46714099271671	38.0	35.0	39.0	18.0	40.0
6	32.613859301982906	38.0	33.0	39.0	12.0	40.0
7	33.97991832610617	38.0	33.0	39.0	23.0	40.0
8	34.19972214036122	38.0	33.0	39.0	25.0	40.0
9	34.152528101713465	38.0	34.0	39.0	24.0	40.0
10	33.01633477876479	38.0	32.0	39.0	18.0	40.0
11	32.548856986485916	37.0	30.0	39.0	18.0	40.0
12	31.612512103734264	36.0	28.0	39.0	15.0	40.0
13	30.736749042226243	35.0	26.0	39.0	12.0	40.0
14	31.740748537026903	36.0	29.0	39.0	15.0	40.0
15	30.115269650149454	35.0	25.0	38.0	10.0	40.0
16	31.352839641308467	36.0	28.0	39.0	14.0	40.0
17	30.280680335115566	35.0	26.0	38.0	12.0	40.0
18	29.89912853113291	35.0	24.0	38.0	11.0	40.0
19	31.02972256136067	35.0	27.0	38.0	14.0	40.0
20	31.608849408495768	36.0	28.0	39.0	17.0	40.0
21	32.23319159685092	37.0	30.0	39.0	16.0	40.0
22	31.68707110680756	36.0	29.0	39.0	13.0	40.0
23	32.35965983244222	37.0	30.0	39.0	16.0	40.0
24	31.66825243127184	36.0	29.0	39.0	14.0	40.0
25	32.11160695491096	37.0	30.0	39.0	16.0	40.0
26	32.467604092114684	37.0	31.0	39.0	15.0	40.0
27	32.688586704837284	38.0	32.0	39.0	16.0	40.0
28	32.68113501452448	38.0	32.0	39.0	16.0	40.0
29	32.226371405717174	37.0	31.0	39.0	13.0	40.0
30	31.954111059655624	36.0	30.0	39.0	12.0	40.0
31	32.66016924177998	37.0	32.0	39.0	12.0	40.0
32	32.54203679535217	37.0	32.0	39.0	11.0	40.0
33	32.359744032332756	37.0	32.0	39.0	10.0	40.0
34	32.05464572896055	36.0	31.0	39.0	9.0	40.0
35	32.23091819980634	37.0	32.0	39.0	4.0	40.0
36	31.22552940681177	36.0	30.0	38.0	4.0	40.0
37	30.970824737927842	35.0	30.0	38.0	4.0	40.0
38	30.48511766934703	35.0	29.0	38.0	4.0	40.0
39	29.323201279838337	35.0	26.0	38.0	4.0	40.0
40	27.113669852229194	33.0	17.0	38.0	4.0	39.0
41	25.03574285353429	30.0	11.0	36.0	4.0	39.0
42	25.972466635793374	31.0	17.0	36.0	4.0	39.0
43	26.263082557992675	31.0	17.0	37.0	4.0	39.0
44	25.525617816696837	31.0	14.0	36.0	4.0	39.0
45	26.428914242411484	32.0	18.0	37.0	4.0	39.0
46	24.363827727023956	29.0	11.0	35.0	4.0	39.0
47	23.664842335704964	28.0	7.0	35.0	4.0	39.0
48	24.041931545488993	28.0	9.0	35.0	4.0	39.0
49	23.13674062223719	28.0	4.0	35.0	4.0	39.0
50	23.897991832610618	29.0	4.0	35.0	4.0	39.0
51	24.086641687365805	30.0	4.0	35.0	4.0	39.0
52	23.995916305308803	30.0	4.0	35.0	4.0	39.0
53	23.889571843556602	30.0	4.0	36.0	4.0	39.0
54	23.511388035195555	29.0	4.0	35.0	4.0	39.0
55	23.357639035069255	29.0	4.0	36.0	4.0	39.0
56	22.375952511261737	28.0	4.0	35.0	4.0	38.0
>>END_MODULE
>>Per tile sequence quality	fail
#Tile	Base	Mean
1	1	3.0205963665006763
1	2	5.625626162128434
1	3	4.8461442756043525
1	4	5.251549654546132
1	5	6.176319946858797
1	6	8.381043449346194
1	7	6.032577411079899
1	8	6.202697847799165
1	9	5.783646065831746
1	10	6.854704120962854
1	11	2.896202954174754
1	12	-6.272498760158879
1	13	-15.617814910874205
1	14	-4.556050220749789
1	15	2.57424042268768
1	16	-2.2452360797473503
1	17	-4.312574060134196
1	18	-3.455949862519727
1	19	0.47831629272745246
1	20	-0.5133327353695485
1	21	5.94036943938978
1	22	6.037189793538079
1	23	6.363729017039262
1	24	6.958362895238825
1	25	5.477046494313797
1	26	6.407901933137666
1	27	5.777555299902797
1	28	5.444290048034318
1	29	5.288141829759269
1	30	5.257887288018484
1	31	4.146041460074223
1	32	5.2478328960214355
1	33	4.611284295199361
1	34	5.303368966426397
1	35	4.221791609755019
1	36	0.7235874757688521
1	37	0.9865511505454414
1	38	0.523123370375103
1	39	1.1314018521425773
1	40	-12.290713549506204
1	41	-3.3406468638168896
1	42	-4.718696999976199
1	43	-9.753506132882114
1	44	-15.379763151090128
1	45	-7.414806126108175
1	46	-9.598132526919283
1	47	-13.556903028610076
1	48	-8.807040130976704
1	49	-14.370773780936705
1	50	-8.540613440877838
1	51	-3.6641896462212706
1	52	-4.053094219596762
1	53	-3.546033682590412
1	54	-3.1972001027560886
1	55	-3.3850436587132364
1	56	0.5376019605851035
2	1	2.981896676098202
2	2	5.689093654388493
2	3	5.191345513994449
2	4	5.260837580242722
2	5	6.3280227332365016
2	6	8.17670908402112
2	7	5.961369980739342
2	8	5.828084844703184
2	9	6.192314796481902
2	10	6.320648393408678
2	11	6.41787478080014
2	12	1.4364795680144944
2	13	3.0803275039864744
2	14	7.213299624451448
2	15	7.5123209180437165
2	16	3.5813893072495517
2	17	7.17813801416921
2	18	4.14776530775891
2	19	6.410204837619094
2	20	5.3380604534849425
2	21	6.564208448677707
2	22	6.825115490132507
2	23	5.36992096750366
2	24	6.992418622793
2	25	6.209244636728656
2	26	6.652483976481314
2	27	5.47724570237958
2	28	6.037169305000262
2	29	6.458420467530168
2	30	7.041169021764613
2	31	5.42622721858816
2	32	5.763312772182431
2	33	5.411593892722578
2	34	4.811108904506888
2	35	5.127364365172976
2	36	5.887674163075356
2	37	6.2559009957466785
2	38	5.9906156304370235
2	39	7.071030335114713
2	40	4.73715022758358
2	41	9.925607006152152
2	42	9.086256560395316
2	43	9.217082102412004
2	44	-2.715676463783627
2	45	8.198196969867059
2	46	7.6046538507896955
2	47	5.303778085941008
2	48	7.504105379859208
2	49	0.49764727169487344
2	50	7.4779624105153495
2	51	10.82187846523384
2	52	10.496441384118409
2	53	10.828579320505565
2	54	11.195988751733072
2	55	11.557680799491099
2	56	12.554629824362195
3	1	3.646851746701536
3	2	5.75905514347707
3	3	5.546287747627311
3	4	5.351980070614999
3	5	6.3979842223250785
3	6	8.988647466562838
3	7	6.490253164308015
3	8	6.156069440338619
3	9	6.348925836276507
3	10	6.958003977490836
3	11	6.870377990042755
3	12	7.243283162366229
3	13	8.825513640058364
3	14	8.527163552564414
3	15	2.668931957838325
3	16	7.680875828430551
3	17	8.80650771891889
3	18	9.876905230737083
3	19	8.08735503017365
3	20	7.989536705089563
3	21	8.21440100323483
3	22	9.089557081916848
3	23	7.8737720586461535
3	24	8.586770355784015
3	25	8.012197139937896
3	26	7.268016710756029
3	27	6.497784855139525
3	28	6.985179574576641
3	29	7.369203522729137
3	30	7.808819856167691
3	31	6.350489092785843
3	32	5.83391610979475
3	33	6.244713276547998
3	34	5.334215451361835
3	35	5.940586444762193
3	36	7.050703688107443
3	37	7.386838094591354
3	38	8.561860816573091
3	39	8.832262684280309
3	40	10.730089893822349
3	41	12.700960022840217
3	42	13.184459384528825
3	43	12.070740638997368
3	44	10.752231110028951
3	45	11.664820846632143
3	46	13.39541123204772
3	47	14.278104144991069
3	48	13.95981783172057
3	49	14.397518901990122
3	50	14.513905927845258
3	51	12.599798876016894
3	52	12.588225723014428
3	53	12.195716676089642
3	54	12.111264746598284
3	55	12.613521621057206
3	56	13.15668373963819
4	1	3.7085048632327045
4	2	6.284122894154578
4	3	5.708889373643572
4	4	5.839784948663777
4	5	6.284163084113693
4	6	8.634311423202405
4	7	6.300551267289045
4	8	6.671652096165175
4	9	6.638221229230439
4	10	7.670063598087037
4	11	8.463196418227042
4	12	8.9350175797104
4	13	9.736760252524487
4	14	8.974995530884197
4	15	9.930449573014474
4	16	8.968816207834351
4	17	10.76585731241482
4	18	10.196010921793995
4	19	9.247924135864704
4	20	8.3249025587481
4	21	8.505728916513963
4	22	9.383595022296252
4	23	7.0614414353399155
4	24	7.587447862559085
4	25	7.821817736143856
4	26	5.759209122680147
4	27	4.92461412343221
4	28	5.076642989210789
4	29	5.655788888582798
4	30	6.143508203051159
4	31	5.0022506104010205
4	32	4.728225052884184
4	33	5.575336582781063
4	34	4.904676155968879
4	35	5.602510564003385
4	36	6.032411005180617
4	37	6.46204134662387
4	38	6.575410952074453
4	39	7.1967613292667565
4	40	1.3351034439578484
4	41	12.105431567555659
4	42	11.039472934664328
4	43	11.772637657967561
4	44	11.929060378321637
4	45	10.492056618989867
4	46	13.272782505760453
4	47	10.925123115180774
4	48	13.704397777520029
4	49	13.851448441285516
4	50	12.80252381402412
4	51	13.314568523713373
4	52	12.834160682364018
4	53	12.957234291265795
4	54	12.232538459335412
4	55	13.043060916450163
4	56	11.449366666467455
5	1	2.927949307677146
5	2	4.778567338599018
5	3	5.200556040310239
5	4	5.16478494866378
5	5	6.46749641744703
5	6	8.21486697875796
5	7	5.656106822844599
5	8	5.9688743183874
5	9	5.515999007008219
5	10	6.406174709198151
5	11	7.727085307115928
5	12	7.7627953574881765
5	13	8.089538030302268
5	14	7.327773308661978
5	15	8.911005128570029
5	16	8.157705096723234
5	17	8.774190645748153
5	18	10.02934425512733
5	19	9.106257469198038
5	20	8.0749025587481
5	21	7.641840027625076
5	22	7.047483911185136
5	23	7.42255254645103
5	24	6.931892307003526
5	25	8.032928847254972
5	26	6.006431344902367
5	27	6.449614123432209
5	28	5.801642989210791
5	29	6.480788888582801
5	30	7.4740637586067145
5	31	5.057806165956578
5	32	5.769891719550849
5	33	5.089225471669948
5	34	5.5018983781911
5	35	5.149732786225609
5	36	7.457411005180614
5	37	6.395374679957207
5	38	6.5892998409633385
5	39	8.232872440377868
5	40	8.998992332846736
5	41	10.088764900888993
5	42	10.178361823553214
5	43	10.067082102412005
5	44	9.404060378321638
5	45	8.583723285656529
5	46	9.57833806131601
5	47	9.969567559625219
5	48	10.948842221964469
5	49	10.004226219063295
5	50	10.735857147357457
5	51	10.028457412602258
5	52	9.867494015697353
5	53	9.712789846821352
5	54	9.38809401489097
5	55	9.995838694227942
5	56	9.174366666467456
6	1	3.541838196566033
6	2	5.784122894154578
6	3	5.236667151421351
6	4	5.895340504219334
6	5	6.617496417447029
6	6	8.523200312091298
6	7	6.4949957117334876
6	8	6.33831876283184
6	9	6.388221229230439
6	10	6.8089524869759295
6	11	8.129863084893707
6	12	9.073906468599294
6	13	9.458982474746708
6	14	8.16943997532864
6	15	10.236005128570032
6	16	8.218816207834351
6	17	10.321412867970377
6	18	11.30712203290511
6	19	9.859035246975818
6	20	7.8249025587481
6	21	8.450173360958413
6	22	9.328039466740695
6	23	8.533663657562144
6	24	8.809670084781306
6	25	9.016262180588306
6	26	7.759209122680147
6	27	7.313503012321096
6	28	7.271087433655232
6	29	6.211344444138355
6	30	6.449063758606716
6	31	5.363361721512135
6	32	5.61711394177307
6	33	6.18644769389217
6	34	5.849120600413322
6	35	5.408066119558942
6	36	7.976855449625059
6	37	7.628708013290542
6	38	7.7142998409633385
6	39	9.668983551488985
6	40	8.001770110624516
6	41	13.466542678666773
6	42	11.8172507124421
6	43	12.30041543574534
6	44	13.345727044988301
6	45	11.93650106343431
6	46	13.717226950204896
6	47	14.147345337402996
6	48	12.759953333075586
6	49	13.295892885729959
6	50	11.774746036246341
6	51	12.759012968157815
6	52	12.55638290458624
6	53	12.957234291265795
6	54	14.121427348224305
6	55	13.404172027561277
6	56	11.588255555356344
7	1	2.791838196566033
7	2	5.395234005265685
7	3	4.958889373643572
7	4	4.700896059774891
7	5	5.756385306335915
7	6	8.273200312091298
7	7	5.467217933955716
7	8	5.921652096165175
7	9	5.582665673674882
7	10	6.447841375864815
7	11	7.102085307115928
7	12	7.907239801932622
7	13	8.542315808080044
7	14	8.058328864217533
7	15	8.76378290634781
7	16	7.635482874501015
7	17	9.932523979081491
7	18	10.140455366238445
7	19	8.414590802531375
7	20	8.0749025587481
7	21	7.672395583180634
7	22	8.911372800074023
7	23	7.783663657562144
7	24	8.781892307003528
7	25	7.988484402810528
7	26	6.814764678235704
7	27	5.146836345654432
7	28	6.437754100321904
7	29	6.711344444138355
7	30	7.254619314162273
7	31	2.418917277067692
7	32	4.339336163995291
7	33	3.353114360558834
7	34	4.404676155968879
7	35	4.296955008447828
7	36	5.976855449625059
7	37	6.267596902179427
7	38	6.519855396518896
7	39	6.4467613292667565
7	40	8.473992332846738
7	41	11.02209823422233
7	42	10.039472934664328
7	43	9.883748769078668
7	44	10.234615933877194
7	45	8.658723285656531
7	46	10.439449172427125
7	47	11.508456448514103
7	48	10.17661999974225
7	49	10.518115107952184
7	50	10.080301591801899
7	51	10.147901857046701
7	52	9.639716237919576
7	53	10.873900957932467
7	54	10.78809401489097
7	55	9.820838694227941
7	56	9.810477777578566
8	1	3.584767489495327
8	2	5.220991581023263
8	3	5.527071191825385
8	4	5.93069403957287
8	5	6.82961762956824
8	6	8.848957887848869
8	7	6.159137125874906
8	8	6.128722803235881
8	9	6.544786885796093
8	10	7.273598951622393
8	11	8.147539852570475
8	12	9.008249902942726
8	13	9.446356212120445
8	14	8.38156118744985
8	15	9.652671795236696
8	16	9.122856611874752
8	17	9.462827009384522
8	18	10.324798800581878
8	19	10.028227166167735
8	20	8.453690437535975
8	21	8.662294573079624
8	22	9.136120274821504
8	23	7.74073436463285
8	24	8.600074125185348
8	25	6.5768682411943615
8	26	6.822340435811462
8	27	6.545826244644335
8	28	5.508461171028969
8	29	6.875485858279767
8	30	6.593003152546107
8	31	4.966897075047484
8	32	5.334285658944786
8	33	6.070286077730557
8	34	5.535989287282007
8	35	5.3929146044074265
8	36	6.87332009608971
8	37	6.227192861775386
8	38	6.49460287126637
8	39	8.565448197953629
8	40	4.004295363149769
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116	34	4.852704829804004
116	35	4.574732786225606
116	36	7.298540037438681
116	37	6.626019841247526
116	38	6.14171919580205
116	39	7.775614375861743
116	40	9.23205684897577
116	41	9.575861675082546
116	42	8.879974726779022
116	43	2.249340166928132
116	44	2.614544249289377
116	45	5.4248523179145955
116	46	-1.4700490354581817
116	47	-7.154625988761879
116	48	-2.052770681261336
116	49	-4.967547974485093
116	50	-0.7189815623199642
116	51	0.9695864448603224
116	52	0.44975208021348223
116	53	0.8353704919826441
116	54	0.36067466005226123
116	55	0.7724515974537489
116	56	4.707431182596487
117	1	0.7665856713135142
117	2	2.8876582476899273
117	3	2.46646513121933
117	4	3.0670576759365034
117	5	3.7084055083561225
117	6	3.7125942514852355
117	7	2.7197431864809687
117	8	1.91660159111467
117	9	2.529635370644584
117	10	2.4402656182890574
117	11	-1.2615510565204353
117	12	-6.537204642511821
117	13	-6.599098333334098
117	14	-3.9972266913380246
117	15	-9.332176689611785
117	16	-8.93774944873131
117	17	-3.8553548087972977
117	18	-5.857019381236306
117	19	-1.5778334398928706
117	20	-2.83418835034281
117	21	2.298658209443257
117	22	4.317938456639684
117	23	2.6043707282692097
117	24	3.690983216094434
117	25	3.8647470290731505
117	26	3.140522253993275
117	27	4.10643230525039
117	28	3.1902793528471562
117	29	4.360334343128251
117	30	2.3808819404248958
117	31	1.6032607114111244
117	32	1.803982628641755
117	33	2.6005891080335815
117	34	1.854171105463827
117	35	1.0292782407710597
117	36	3.191501914271523
117	37	3.4544655890481124
117	38	3.5097543864178853
117	39	4.671508804014234
117	40	6.473992332846738
117	41	5.461492173616268
117	42	1.4283618235532138
117	43	-5.782917897587996
117	44	-1.6845759853147264
117	45	2.8632687402019847
117	46	-3.9216619386839895
117	47	-1.9258869858293295
117	48	-2.2829759598537116
117	49	-0.41622832639125207
117	50	-1.500506489006181
117	51	4.408002867147712
117	52	5.417494015697354
117	53	5.558244392275899
117	54	6.629003105800059
117	55	4.729929603318851
117	56	8.267548484649272
118	1	2.3553302600580963
118	2	4.692853052884736
118	3	4.089841754595952
118	4	3.649308758187587
118	5	5.379401179351788
118	6	7.273200312091298
118	7	5.1894401561779375
118	8	4.977207651720732
118	9	4.665999007008217
118	10	4.126412804436246
118	11	1.3639900690206908
118	12	1.1770810717738946
118	13	-3.1640333982691615
118	14	-3.354369548480882
118	15	-13.061613919049016
118	16	-8.916104427086289
118	17	-0.376999830442319
118	18	-6.411131935348859
118	19	-2.04969491175434
118	20	-1.3774783936328525
118	21	4.8311257419107925
118	22	5.010579149280378
118	23	5.589219213117694
118	24	6.210463735574958
118	25	5.2781669424930655
118	26	5.207621821092843
118	27	5.662709361527451
118	28	5.326642989210789
118	29	5.953407936201849
118	30	5.5800161395590955
118	31	4.343520451670862
118	32	4.5377488624079945
118	33	5.035654043098518
118	34	3.245945997238721
118	35	3.860447071939891
118	36	5.210982433752047
118	37	5.4263270609095855
118	38	6.845252221915718
118	39	7.76025339275882
118	40	9.307325666180073
118	41	9.71257442469852
118	42	6.856933252124641
118	43	2.836129721459624
118	44	8.25048894975021
118	45	7.194437571370816
118	46	-0.01690003392208439
118	47	6.4290913691490275
118	48	1.6845565076787565
118	49	2.605416695253769
118	50	5.207285718786029
118	51	8.739171698316543
118	52	9.32225592045926
118	53	9.36993270396421
118	54	9.990474967271922
118	55	9.797029170418416
118	56	13.78269999980079
119	1	2.7211311258589674
119	2	5.069476429508114
119	3	4.693737858492057
119	4	5.157966766845597
119	5	5.935678235628849
119	6	7.530776069667056
119	7	5.628834095571875
119	8	5.59841977293285
119	9	5.620544461553671
119	10	5.7584474364708775
119	11	3.238448943479561
119	12	-0.03720464251182065
119	13	-1.0081892424250078
119	14	-2.269953964065298
119	15	-6.059449416884515
119	16	-5.346840357822217
119	17	-0.30990026334275456
119	18	-6.175201199418122
119	19	-1.1687425308019606
119	20	-0.3796428957973532
119	21	4.8895673003523505
119	22	5.045211183912411
119	23	4.695279819178303
119	24	3.7818923070035275
119	25	4.773837938164064
119	26	5.867794981266002
119	27	6.333705032523117
119	28	6.1448248073926095
119	29	6.496697979491891
119	30	7.108154667697622
119	31	6.012351620502031
119	32	6.985800810459942
119	33	6.418770926215402
119	34	5.990534741827467
119	35	6.620187331680153
119	36	8.327865550635163
119	37	8.31810195268448
119	38	8.373390750054245
119	39	9.48969062219605
119	40	11.428537787392191
119	41	9.052401264525358
119	42	7.473816369007761
119	43	1.7170821024120038
119	44	3.542696741958
119	45	7.226905103838348
119	46	3.260156243134194
119	47	1.5286584687161273
119	48	-2.9193395962173447
119	49	2.311044400881478
119	50	5.181311692812002
119	51	9.680730139874985
119	52	10.281130379333717
119	53	11.467335301366806
119	54	10.401730378527333
119	55	10.593565966955214
119	56	11.494821211922002
120	1	1.9246884381119287
120	2	4.587262990772935
120	3	3.8103386490058853
120	4	4.318045818228995
120	5	5.269670330490506
120	6	7.418127848323181
120	7	4.595237257627211
120	8	4.296048231430873
120	9	4.361651180921264
120	10	4.379000796154674
120	11	0.47165052450723266
120	12	-4.145900294685735
120	13	-3.3441576218716484
120	14	-4.1059223435119385
120	15	0.8990486068309025
120	16	-5.740120990233287
120	17	-0.6399397890344538
120	18	-4.307612266611798
120	19	0.33125746919803944
120	20	0.31403299353070935
120	21	1.2907530711033388
120	22	2.4681360850981804
120	23	3.487769937755381
120	24	1.9558053504817892
120	25	2.0959723255158416
120	26	4.774909605771931
120	27	3.2072228190843823
120	28	3.8483821196455708
120	29	4.601441062495844
120	30	4.720802889041497
120	31	3.5360670355217962
120	32	3.91663084998563
120	33	3.6816167760177763
120	34	3.061680986886749
120	35	3.5312545253560437
120	36	3.6519762225719212
120	37	4.306244245174597
120	38	3.6816911453111665
120	39	5.286133309943089
120	40	7.147905376324996
120	41	-8.637322055632744
120	42	-6.136855567751134
120	43	-9.261178767153213
120	44	-13.451374404287058
120	45	-3.26518975782173
120	46	-8.226009764770946
120	47	-6.293475918635652
120	48	-6.0359403867311805
120	49	-12.479469433110618
120	50	-6.016316765686023
120	51	0.49693567347182466
120	52	-0.8433755495200366
120	53	-0.35677537056995234
120	54	0.2989635801083601
120	55	0.19040391161924575
120	56	2.8841492751631073
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
4	1292.0
5	127.0
6	95.0
7	59.0
8	53.0
9	47.0
10	66.0
11	45.0
12	45.0
13	66.0
14	77.0
15	91.0
16	95.0
17	133.0
18	207.0
19	242.0
20	346.0
21	443.0
22	488.0
23	584.0
24	589.0
25	696.0
26	739.0
27	729.0
28	755.0
29	839.0
30	940.0
31	1069.0
32	1215.0
33	1340.0
34	1469.0
35	1638.0
36	1698.0
37	1876.0
38	1876.0
39	1680.0
40	4.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.0	0.0	0.0	100.0
2	0.0	100.0	0.0	0.0
3	0.0	100.0	0.0	0.0
4	0.0	0.0	0.0	100.0
5	0.0	100.0	0.0	0.0
6	0.0	100.0	0.0	0.0
7	96.34993474508484	3.0564560266071656	0.09682987412116363	0.49677935418683955
8	96.29941481076074	2.925946196269945	0.10524986317517787	0.6693891297941312
9	95.66352307174132	3.094476254631189	0.6146850791512294	0.6273155944762546
10	40.25175767271503	26.059866122174043	26.22826590325433	7.460110301856608
11	25.356797036163854	27.739653938449884	16.646318359786132	30.257230665600133
12	23.719109165158088	31.69283879930956	17.315707489580266	27.272344545952095
13	18.978655327748072	16.010609186208058	20.140613817202038	44.870121668841826
14	18.608175809371446	37.30055150928304	23.588599334820863	20.50267334652465
15	39.44138806601777	19.809564113415153	19.432924248836226	21.31612357173085
16	25.5845304798416	36.68113072418587	19.151535577368666	18.582803218603868
17	21.89144806097099	19.55450755821298	41.43753421196682	17.116510168849214
18	19.01233528396413	19.59752452321812	24.48532816907338	36.90481202374437
19	23.369130343230154	21.179195620130553	18.22699515687513	37.22467887976416
20	19.643384057665557	26.164481726594445	34.44336719639169	19.748767019348314
21	18.726844055237454	20.137251599865273	24.81475244189963	36.32115190299764
22	16.730518250326273	19.542794594367027	37.729970951037764	25.99671620426893
23	17.18940765377005	22.334020965772744	35.86494337557361	24.611628004883592
24	16.214054145088628	23.29586122689571	24.432655467138225	36.05742916087743
25	23.733524234640164	23.068177032888364	33.2420937381564	19.956204994315073
26	37.77113254432886	22.309733395105926	16.800741271111484	23.118392789453736
27	20.13649001600809	37.40837475777235	16.441991743196564	26.013143483023
28	27.306024502168146	34.46301519808024	18.1071864606576	20.12377383909401
29	32.9439055250949	30.679038380430196	17.633909742724587	18.74314635175032
30	27.768887391560682	22.041607007496	31.68954771329908	18.499957887644236
31	28.9527980125479	33.47930439176386	20.127163248978906	17.440734346709334
32	32.45735944409349	31.22341545588545	18.879764160875972	17.439460939145082
33	30.65059834822181	28.36676217765043	18.565649755604248	22.41698971852351
34	20.221022439682805	26.138856082335078	35.29188459591699	18.348236882065123
35	18.32363605700312	22.92773421030441	41.42845096551142	17.320178767181044
36	27.097725358045494	26.457455770850885	28.29401853411963	18.150800336983995
37	26.679875221313548	21.250316162212293	33.31506618328977	18.754742433184386
38	31.977553689717737	22.79228724526391	27.640183958482766	17.589975106535586
39	28.201450987008602	22.414374894550363	19.26353973342332	30.120634385017713
40	22.3028913514202	17.967987092939328	30.246677705600135	29.482443850040337
41	18.658780477426493	16.2461171865027	27.245649121313985	37.84945321475682
42	22.876203344029726	29.31937172774869	21.178854923154873	26.625570005066713
43	16.066903193106942	26.99780368305457	31.284845413076535	25.650447710761952
44	18.330231771273898	29.11943833530706	26.298426662155304	26.251903231263746
45	24.909275044307535	22.145328719723185	32.6272259262385	20.318170309730778
46	17.677130423761035	29.426676243187295	21.559846212345263	31.33634712070641
47	15.078055590811015	28.58230739941617	21.614418073359566	34.72521893641325
48	26.234619922467555	27.119501095567166	21.28771279285353	25.358166189111746
49	17.478885135135137	30.684121621621625	20.004222972972975	31.832770270270274
50	21.656050955414013	28.961909984392797	21.86695912599654	27.515079934196653
51	17.652772498838633	32.12973520841252	23.586300097132483	26.63119219561637
52	17.845842298443234	37.10500780491921	25.304813736657806	19.74433615997975
53	19.03254400405217	34.40547043180955	26.972267949854373	19.589717614283906
54	18.762930124551406	27.17753852649356	29.119696010132994	24.93983533882204
55	16.63641352526489	26.421545865169488	29.92528177635189	27.01675883321373
56	21.622420124087284	22.39480015194361	31.355252606255014	24.62752711771409
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	9.0
4	28.0
5	38.0
6	40.0
7	42.0
8	46.0
9	50.0
10	68.5
11	87.0
12	99.5
13	121.5
14	131.0
15	125.0
16	119.0
17	122.5
18	126.0
19	118.0
20	110.0
21	104.5
22	112.0
23	125.0
24	154.0
25	183.0
26	180.0
27	177.0
28	183.5
29	210.5
30	231.0
31	240.5
32	250.0
33	279.5
34	309.0
35	304.5
36	300.0
37	350.0
38	441.0
39	482.0
40	549.0
41	616.0
42	877.0
43	1138.0
44	1869.5
45	2601.0
46	2587.0
47	2135.0
48	1697.0
49	1499.0
50	1301.0
51	1308.0
52	1315.0
53	1348.0
54	1250.0
55	1119.0
56	1167.0
57	1215.0
58	1160.5
59	1106.0
60	1047.0
61	988.0
62	908.5
63	760.0
64	691.0
65	581.0
66	471.0
67	415.5
68	360.0
69	316.0
70	272.0
71	234.0
72	145.5
73	95.0
74	79.0
75	63.0
76	57.5
77	52.0
78	59.0
79	45.5
80	25.0
81	14.5
82	4.0
83	2.0
84	0.0
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.004209994527007115
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.5178293268218751
16	0.06735991243211384
17	0.01683997810802846
18	0.0
19	0.03367995621605692
20	0.12629983581021345
21	0.004209994527007115
22	0.0
23	0.0
24	0.00841998905401423
25	0.025259967162042693
26	0.042099945270071146
27	0.06314991790510673
28	0.0
29	0.18102976466130594
30	0.029469961689049803
31	0.01683997810802846
32	0.03367995621605692
33	0.08840988506714942
34	0.18944975371532016
35	0.14734980844524903
36	0.05472992885109249
37	0.13050983033722055
38	0.21891971540436997
39	0.18944975371532016
40	0.841998905401423
41	1.060918620805793
42	0.2904896223634909
43	0.3241695785795478
44	0.45888940344377555
45	0.2315496989853913
46	0.3536395402685977
47	0.4883593651328253
48	0.08840988506714942
49	0.3073296004715194
50	0.19365974824232726
51	0.3115395949985265
52	0.21049972635035574
53	0.2610196606744411
54	0.2862796278364838
55	0.26943964972845535
56	0.2525996716204269
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
56	23753.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	71.52780701385089
#Duplication Level	Percentage of deduplicated	Percentage of total
1	89.0935844614479	63.72668715530669
2	6.3802236609770455	9.127268134551425
3	1.7598587404355504	3.7763650907253825
4	0.8298999411418482	2.374436913232013
5	0.5709240729841083	2.0418473455984505
6	0.3649205414949971	1.566117964046647
7	0.2177751618599176	1.0903885824948427
8	0.12360211889346676	0.7072790805371953
9	0.08828722778104767	0.5683492611459605
>10	0.5120659211300765	6.6054814128741635
>50	0.023543260741612712	1.0440786426977644
>100	0.02942907592701589	4.900433629436281
>500	0.005885815185403178	2.4712667873531764
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CAACAAGGGTCACAGATCCTCTTCTGAGATGAGTTTTTGTTCAATCCGCCCAATCT	587	2.4712667873531764	No Hit
CAACAAGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAG	438	1.8439776028291162	No Hit
CAACAAGGGTCACAGATCCTCTTCTGAAGGAGATTGGGCGGATTGAACAAAAACTC	320	1.3471982486422767	No Hit
CAACAAGGGTCACAGATCCTCTTCTGAGATGAGTTTTTGTCCAATCCGCCCAATCT	155	0.6525491516861028	No Hit
CAACAAGGGTCACAGATCCTCTTCTGAAGGAGATTGGGCGCATTGAACAAAAACTC	142	0.5978192228350103	No Hit
CAACAAGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTCGTCTCAAAGATTAAG	109	0.45888940344377555	No Hit
CAACAAGGGGGGCGCTGACCCCCCTTCCCGGGGGGGGATGCGTGCATTTATCAGAT	71	0.29890961141750516	No Hit
CAACAAGGGTCACAGATCCTCTTCTGAGATGAGTTTTTGTTCAATCCGCCCAAAAA	61	0.256809666147434	No Hit
CAACAAGGGTACCTGGTTGATCCTGCCAGTAGCATATGCCTGTCTCAAAGATTAAG	58	0.24417968256641265	No Hit
CAACAAGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCCAAGATTAAG	58	0.24417968256641265	No Hit
CAACAAGGGAACTGTGGTAATTCTAGAGCTAATACATGCCGACGGGCGCTGACCCC	50	0.21049972635035574	No Hit
CAACAAGGGTCACAGATCCTCTTCTGAAGGAGATTGGGCCGATTGAACAAAAACTC	48	0.20207973729634154	No Hit
CAACAAGGGATGGTTCCTTTGGTCGCTCGCTCCTCTCCTACTTGGATAACTGTGGT	47	0.1978697427693344	No Hit
CAACAAGGGGGTTCCTTTGGTCGCTCGCTCCTCTCCTACTTGGATAACTGTGGTAA	40	0.16839978108028458	No Hit
CAACAAGGGTCACAGATCCTCTTCTGAGATGAGTTTTTGCTCAATCCGCCCAATCT	39	0.16418978655327748	No Hit
CAACAAGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCCACGATTAAG	36	0.15155980297225613	No Hit
CAACAAGGGAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGTC	36	0.15155980297225613	No Hit
CAACAAGGGGAACGTCTGCCCTATCAACTTTCGATGGTAGTCGCCGTGCCTACCAT	35	0.14734980844524903	No Hit
CAACAAGGGGGTGAGCTCCCTCCCGGCTCCGGCCGGGGGTCGGGCGCCGGCGGCTT	35	0.14734980844524903	No Hit
CAACAAGGGCTCCTACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCGAC	33	0.1389298193912348	No Hit
CAACAAGGGAGTTATGGTTCCTTTGGTCGCTCGCTCCTCTCCTACTTGGATAACTG	32	0.13471982486422768	No Hit
CAACAAGGGATCAGATCAAAACCAACCCGGTGAGCTCCCTCCCGGCTCCGGCCGGG	32	0.13471982486422768	No Hit
CAACAAGGGTCACAGATCCTCTTCTGAAGGAGATTGGGCGGATTGACCAAAAACTC	32	0.13471982486422768	No Hit
CAACAAGGGTACCTGGTTGATCCTGCCAGTAGCATATGCCGTCTTCTGCTTGGATC	30	0.12629983581021345	No Hit
CAACAAGGTCACAGATCCTCTTCTGAGATGAGTTTTTGTTCAATCCGCCCAATCTC	29	0.12208984128320632	No Hit
CAACAAGGGTCACAGATCCTCTGAAGGAGATTGGGCGGATTGAACAAAAACTCATC	28	0.11787984675619921	No Hit
CAACAAGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	28	0.11787984675619921	No Hit
CAACAAGGGTCACAGATCCTCTCCTGAAGGAGATTGGGCGGATTGAACAAAAACTC	26	0.10945985770218498	No Hit
CAACAAGGGAACTGTGGTAATTCTAGAGCTAATACATGCCCACGGGCGCTGACCCC	25	0.10524986317517787	No Hit
CAACAAGGGGTCACAGATCCTCTTCTGAGATGAGTTTTTGTTCAATCCGCCCAATC	24	0.10103986864817077	No Hit
CAACAAGGGGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCCATTCGAACGTC	24	0.10103986864817077	No Hit
CAACAAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC	24	0.10103986864817077	No Hit
CAACAAGGGGTGGCGGCGACGACCCATTCGAACGTCTGCCCTATCAACTTTCGATG	24	0.10103986864817077	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.012629983581021345	0.0	0.0	0.0	0.0
12	0.021049972635035573	0.0	0.0	0.0	0.0
13	0.021049972635035573	0.0	0.0	0.0	0.0
14	0.058939923378099605	0.0	0.0	0.0	0.0
15	0.058939923378099605	0.0	0.0	0.0	0.0
16	0.06314991790510673	0.0	0.0	0.0	0.0
17	0.06735991243211384	0.0	0.0	0.0	0.0
18	0.06735991243211384	0.0	0.0	0.0	0.0
19	0.06735991243211384	0.0	0.0	0.0	0.0
20	0.07156990695912095	0.0	0.0	0.0	0.0
21	0.07577990148612807	0.0	0.0	0.0	0.0
22	0.07998989601313518	0.0	0.0	0.0	0.0
23	0.07998989601313518	0.0	0.0	0.0	0.0
24	0.07998989601313518	0.0	0.0	0.0	0.0
25	0.08419989054014229	0.0	0.0	0.0	0.0
26	0.08419989054014229	0.0	0.0	0.0	0.0
27	0.10524986317517787	0.0	0.0	0.0	0.0
28	0.1136698522291921	0.0	0.0	0.0	0.0
29	0.1136698522291921	0.0	0.0	0.0	0.0
30	0.1136698522291921	0.0	0.0	0.0	0.0
31	0.11787984675619921	0.0	0.0	0.0	0.0
32	0.12629983581021345	0.0	0.0	0.0	0.0
33	0.12629983581021345	0.0	0.0	0.0	0.0
34	0.12629983581021345	0.0	0.0	0.0	0.0
35	0.12629983581021345	0.0	0.0	0.0	0.0
36	0.13050983033722055	0.0	0.0	0.0	0.0
37	0.13050983033722055	0.0	0.0	0.0	0.0
38	0.13050983033722055	0.0	0.0	0.0	0.0
39	0.13471982486422768	0.0	0.0	0.0	0.0
40	0.13471982486422768	0.0	0.0	0.0	0.0
41	0.1389298193912348	0.0	0.0	0.0	0.0
42	0.1431398139182419	0.0	0.0	0.0	0.0
43	0.15576979749926326	0.0	0.0	0.0	0.0
44	0.17681977013429884	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTGTCCA	20	3.8341625E-4	50.682404	37
TTGTTCC	20	3.8341625E-4	50.682404	37
GTTCCCT	20	3.8341625E-4	50.682404	39
TGCCTGT	15	0.007893854	50.682404	37
TCCAATC	20	3.8341625E-4	50.682404	40
CTGTCTC	15	0.007893854	50.682404	40
GCCTGTC	15	0.007893854	50.682404	38
GGCGCAT	65	0.0	50.6824	37
ATGCCTG	15	0.007961243	50.57388	36
TTTGCTC	15	0.007961243	50.57388	36
TGTCTCC	20	3.875112E-4	50.573875	41
TTTGTCC	20	3.875112E-4	50.573875	36
TATGCCT	15	0.00802906	50.465813	35
ATATGCC	20	3.9580528E-4	50.358208	34
TCCCAAT	15	0.008235092	50.144375	48
CCCTCCG	20	4.0423987E-4	50.144375	42
GGGCCCT	15	0.008235092	50.144375	33
TCCAAGA	20	4.0851036E-4	50.038136	45
CTCTGAA	20	4.1281662E-4	49.93235	19
CCTATCA	15	0.008374614	49.93235	20
>>END_MODULE