FastQCFastQC Report
Fri 17 Jun 2016
SRR1526173_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526173_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1641
Sequences flagged as poor quality0
Sequence length40
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACAAGGGTACCTGGTTGATCCTGCCAGTAGCATATGCT160.9750152346130408No Hit
CAACAAGGGTACCTGGTTGATCCTGCCCGTAGCATATGCT90.5484460694698354No Hit
CAACAAGGGTCGACTTTTTAGACTGGTGCTGGTGTAAGTA80.4875076173065204No Hit
CAACAAGGGTACCTGGTTGATCCTGCCCGTAGCCTATGCT70.42656916514320536No Hit
CAACAAGGGATGCTAAAAATAAATTTTTGTAAAAGTGAAA70.42656916514320536No Hit
CAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA60.3656307129798903No Hit
CAACAAGGGTACCTGGTTGATCCTGCCAGTAGCCTATGCT50.30469226081657524No Hit
CAACAAGGGTACCTGGTTGATCCTGCCAGTAGCCTCTGCT40.2437538086532602No Hit
CAACAAGGGTACCTGGTTCATCCTGCCAGTAGCATATGCT30.18281535648994515No Hit
CAACAAGGGGCTCTTTCCGTTCCTAGCGCCGCCATGGCTC30.18281535648994515No Hit
CAACAAGGGTACCTGGTTGATCCTGCCCGTAGCATCTGCT30.18281535648994515No Hit
CAACAAGGGGCAGAGTCGCCTTTTTTTTTTTTTTTTTTTT30.18281535648994515No Hit
CAACAAGGGATGAGATGGACTTAAATGAATTGACGGGTAC30.18281535648994515No Hit
CAACAAGGGTCGACTTTTTAGCCTGGTGCTGGTGTAAGTA30.18281535648994515No Hit
CAACAAGGGAACCAAACCCGGTTTTGAGTATTTTCCCATT30.18281535648994515No Hit
CAACAAGGGTACCTGGTTGATCCTGCCCGTCGCATATGCT30.18281535648994515No Hit
CAACAAGGGAATCAACAGCGTTTTTTTTTTTTTTTTTTTT20.1218769043266301No Hit
CAACAAGGGGTTTCCCTCCCTACCCCCTGGGGAGTTGTTT20.1218769043266301No Hit
CAACAAGGGGATGAGATGGACTCACATGAATTGACGGGTA20.1218769043266301No Hit
CAACAAGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCG20.1218769043266301No Hit
CAACAAGGGAAGCTGTATCACGCAGAGTCGACTTTTTTTT20.1218769043266301No Hit
CAACAAGGGCAGTCGACTTTTTTTTTTTTTTTTTTTTTTT20.1218769043266301No Hit
CAACAAGGGCTCGTCTTAATCCGCCACATCTTTTGATACA20.1218769043266301No Hit
CAACAAGGGGTCGACTTTTTTTTTTTTTTTTTTTTTTTTT20.1218769043266301No Hit
CAACAAGGGCAGTACCTTCACTTAGGTATAGACCCCAGTA20.1218769043266301No Hit
CAACAAGGGTGCAACGCAGATCGACTTTTTTTTTTTTTTT20.1218769043266301No Hit
CAACAAGGGTACCTGGTTCATCCTGCCCGTAGCCTATGCT20.1218769043266301No Hit
CAACAAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAG20.1218769043266301No Hit
CAACAAGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTT20.1218769043266301No Hit
CAACAAGGGACACCAGATCGACTTTTTTTTTTTTTTTTTT20.1218769043266301No Hit
CAACAAGGGGCCTTTTTTTTTTTTTTTTTTTTTTTTTTTT20.1218769043266301No Hit
CAACAAGGGTACCTGGTTCATCCTGCCAGTAGCCTATGCT20.1218769043266301No Hit
CAACAAGGGGCATTTGGATGGTTGCAGGGGACTTGCAAAT20.1218769043266301No Hit
CAACAAGGGTACCTGGTTGATCCTGCCAGTAGCATATCCT20.1218769043266301No Hit
CAACAAGGGGGGTTATGTAATTGGAAGCTGCCTCAAACAC20.1218769043266301No Hit
CAACAAGGGGACGATGACTCTTAACGAAGAAAGGTGGCTG20.1218769043266301No Hit
CAACAAGGGGCTTTTCCCCCTCTTCCCTTCGCCAATGAGG20.1218769043266301No Hit
CAACAAGGGTTCAACGCAGAGTCGACTTTTTTTTTTTTTT20.1218769043266301No Hit
CAACAAGGGCACAGCCAAGCTATTATGTTCATTTAACTTC20.1218769043266301No Hit
CAACAAGGGCTGTAACGAGAGTCGATTTTTTTTTTTTTTT20.1218769043266301No Hit
CAACAAGGGAGCATGAGCCGATCTACAAAGCATGGAAACT20.1218769043266301No Hit
CAACAAGGGGGCGGCCGCACTCCAGTCCCTAGCTTGCCGC20.1218769043266301No Hit
CAACAAGGGATAGATGTGGCGACTTGGGTGCGGCTGCTCC20.1218769043266301No Hit
CAACAAGGTGGTATCCAGCTCGCTTTTTTTTTTTTTTTTT20.1218769043266301No Hit
CAACAAGGGGATGAGATGGACTTAAATGAATTGACGGGTA20.1218769043266301No Hit
CAACAAGGGTACCTGGTTGATCCTGCCAGTAGCATCTGCT20.1218769043266301No Hit
CAACAAGGGTTCACGCAGAGTCGATTTTTTTTTTTTTTTT20.1218769043266301No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACAAGG1500.034.483872
AAGGGGG306.7181827E-634.483875
ACAAGGG1500.034.483873
CAAGGGT402.0960215E-834.483874
CAAGGGG650.034.483874
CAAGGGA306.7181827E-634.483874
AAGGGGA251.14247334E-434.4838685
CAACAAG1550.033.406251
Produced by FastQC (version 0.11.5)