FastQCFastQC Report
Fri 17 Jun 2016
SRR1526170_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526170_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24332
Sequences flagged as poor quality0
Sequence length56
%GC56

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCGCAGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAG1970.8096334045701135No Hit
CTCGCAGGGTCACAGATCCTCTTCTGAGATGAGTTTTTGTTCAATCCGCCCAATCT1540.6329113924050633No Hit
CTCGCAGGGGGGCGCTGACCCCCCTTCCCGGGGGGGGATGCGTGCATTTATCAGAT1150.47262863718559917No Hit
CTCGCAGGGTCACAGATCCTCTTCTGAAGGAGATTGGGCGGATTGAACAAAAACTC980.4027617951668585No Hit
CTCGCAGGGAACTGTGGTAATTCTAGAGCTAATACATGCCGACGGGCGCTGACCCC980.4027617951668585No Hit
CTCGCAGGGGGTTCCTTTGGTCGCTCGCTCCTCTCCTACTTGGATAACTGTGGTAA800.32878513891172123No Hit
CTCGCAGGGAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGTC690.28357718231135953No Hit
CTCGCAGGGCTCCTACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCGAC670.27535755383856647No Hit
CTCGCAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTC600.2465888541837909No Hit
CTCGCAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC580.23836922571099783No Hit
CTCGCAGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTCGTCTCAAAGATTAAG530.21782015452901526No Hit
CTCGCAGGGGTGGCGGCGACGACCCATTCGAACGTCTGCCCTATCAACTTTCGATG460.1890514548742397No Hit
CTCGCAGGGGGATAACTGTGGTAATTCTAGAGCTAATACATGCCGACGGGCGCTGA410.16850238369225712No Hit
CTCGCAGGGAGTTATGGTTCCTTTGGTCGCTCGCTCCTCTCCTACTTGGATAACTG380.15617294098306755No Hit
CTCGCAGGGGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCCATTCGAACGTC360.14795331251027452No Hit
CTCGCAGGGGCTCGCTCCTCTCCTACTTGGATAACTGTGGTAATTCTAGAGCTAAT360.14795331251027452No Hit
CTCGCAGGGGGCTACCACATCCAAGGAAGGCAGCAGGCGCGCAAATTACCCACTCC350.14384349827387802No Hit
CTCGCAGGGGGTGAGCTCCCTCCCGGCTCCGGCCGGGGGTCGGGCGCCGGCGGCTT350.14384349827387802No Hit
CTCGCAGGGTACCTGGTTGATCCTGCCAGTAGCATATGCCTGTCTCAAAGATTAAG330.13562386980108498No Hit
CTCGCAGGGAACTGTGGTAATTCTAGAGCTAATACATGCCCACGGGCGCTGACCCC310.12740424132829195No Hit
CTCGCAGGGGGGTGACGGGGAATCAGGGTTCGATTCCGGAGAGGGAGCCTGAGAAA300.12329442709189545No Hit
CTCGCAGGGTCACAGATCCTCTTCTGAGATGAGTTTTTGTCCAATCCGCCCAATCT300.12329442709189545No Hit
CTCGCAGGGGGTGACTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGC300.12329442709189545No Hit
CTCGCAGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTT290.11918461285549892No Hit
CTCGCAGGGAAGCCATGCATGTCTAAGTACGCACGGCCGGTACAGTGAAACTGCGA290.11918461285549892No Hit
CTCGCAGGGATCAGATCAAAACCAACCCGGTGAGCTCCCTCCCGGCTCCGGCCGGG280.11507479861910241No Hit
CTCGCAGGGGGAGAGGGAGCCTGAGAAACGGCTACCACATCCAAGGAAGGCAGCAG280.11507479861910241No Hit
CTCGCAGGGTCACAGATCCTCTTCTGAAGGAGATTGGGCGCATTGAACAAAAACTC280.11507479861910241No Hit
CTCGCAGGGGGCGGCGACGACCCATTCGAACGTCTGCCCTATCAACTTTCGATGGT270.11096498438270591No Hit
CTCGCAGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCCAAGATTAAG270.11096498438270591No Hit
CTCGCAGGGGGTTCCTTTGGTCGCTCGCTCCTCTCCTACTCGGATAACTGTGGTAA270.11096498438270591No Hit
CTCGCAGGGCTCCTACTTGGATAACTGTGGTAATTCTAGACCTAATACATGCCGAC250.10274535590991288No Hit
CTCGCAGGGATCCAAGGAAGGCAGCAGGCGCGCAAATTACCCACTCCCGACCCGGG250.10274535590991288No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCATGCC150.00813733650.29937747
CCCGCGG150.00813733650.29937747
TCCACTC150.00813733650.29937743
CAAGGCA150.00813733650.29937715
CACATGG150.00827231450.091140
GACGGCG150.00827231450.091113
ATGGAGG150.00827231450.091136
CGACGGC150.00827231450.091112
GAGCATA150.00834042449.9876029
TTAAGAG150.00840895149.88453733
TATCAGA252.0549845E-549.88453749
GTTAAGA150.00840895149.88453732
AGGGGTT507.2759576E-1249.7818956
AGGGGTA252.080304E-549.7818956
AGGGTCA850.049.7818956
AGGGCAG252.080304E-549.7818956
AGGGTAC507.2759576E-1249.7818956
AGGGATT600.049.7818956
AGGGATC252.080304E-549.7818956
AGGGAGG252.080304E-549.7818956
Produced by FastQC (version 0.11.5)