##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1526170_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 24332 Sequences flagged as poor quality 0 Sequence length 56 %GC 56 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.326319250369885 38.0 36.0 39.0 30.0 40.0 2 35.10874568469505 38.0 35.0 39.0 25.0 40.0 3 35.11059510110143 38.0 35.0 39.0 25.0 40.0 4 34.409337497945096 38.0 33.0 39.0 23.0 40.0 5 34.73610882788098 38.0 34.0 39.0 23.0 40.0 6 33.06641459806017 38.0 33.0 39.0 12.0 40.0 7 34.88615814565182 38.0 35.0 39.0 26.0 40.0 8 35.02013808975834 38.0 35.0 39.0 27.0 40.0 9 35.04557783988164 38.0 35.0 39.0 27.0 40.0 10 34.11022521782015 38.0 33.0 39.0 22.0 40.0 11 34.06246917639323 38.0 33.0 39.0 23.0 40.0 12 32.74506822291632 37.0 30.0 39.0 18.0 40.0 13 32.23467039289824 37.0 29.0 39.0 16.0 40.0 14 32.96518987341772 38.0 30.0 39.0 19.0 40.0 15 32.01874075291797 37.0 29.0 39.0 15.0 40.0 16 32.56271576524741 37.0 30.0 39.0 19.0 40.0 17 31.540481670228505 36.0 28.0 39.0 15.0 40.0 18 30.88689791221437 35.0 26.0 39.0 14.0 40.0 19 32.138624034193654 36.0 29.0 39.0 18.0 40.0 20 32.607142857142854 36.0 30.0 39.0 20.0 40.0 21 32.70216176228834 37.0 30.0 39.0 18.0 40.0 22 31.93120170968272 36.0 29.0 39.0 15.0 40.0 23 32.64922735492356 37.0 30.0 39.0 18.0 40.0 24 32.0075209600526 36.0 29.0 39.0 16.0 40.0 25 32.406871609403254 36.0 30.0 39.0 18.0 40.0 26 32.92487259575867 37.0 31.0 39.0 18.0 40.0 27 33.116842018740755 38.0 32.0 39.0 19.0 40.0 28 33.05556468847608 37.0 31.0 39.0 20.0 40.0 29 32.592922899884925 37.0 31.0 39.0 18.0 40.0 30 32.319085977313826 36.0 30.0 39.0 17.0 40.0 31 33.22205326319251 37.0 33.0 39.0 19.0 40.0 32 33.09238862403419 37.0 33.0 39.0 19.0 40.0 33 32.85632089429558 37.0 32.0 39.0 18.0 40.0 34 32.72788919940819 36.0 32.0 39.0 18.0 40.0 35 32.61191024165708 37.0 32.0 39.0 17.0 40.0 36 31.888377445339472 36.0 30.0 38.0 12.0 40.0 37 31.60500575373993 36.0 30.0 38.0 10.0 40.0 38 31.05527700147953 35.0 29.0 38.0 4.0 40.0 39 30.185147131349662 35.0 28.0 38.0 4.0 40.0 40 27.73902679598882 33.0 19.0 38.0 4.0 39.0 41 25.94229820812099 31.0 15.0 37.0 4.0 39.0 42 26.8513891172119 31.0 20.0 37.0 4.0 39.0 43 26.73039618609239 32.0 19.0 37.0 4.0 39.0 44 25.934859444353116 31.0 16.0 36.0 4.0 39.0 45 26.839676146638173 32.0 20.0 37.0 4.0 39.0 46 24.814811770507973 29.0 15.0 36.0 4.0 39.0 47 23.781275686338976 28.0 10.0 35.0 4.0 39.0 48 24.393309222423145 29.0 12.0 35.0 4.0 39.0 49 23.40588525398652 28.0 7.0 35.0 4.0 38.0 50 24.06394870951833 29.0 8.0 35.0 4.0 38.0 51 24.20035344402433 30.0 4.0 35.0 4.0 39.0 52 23.954134473121815 29.0 4.0 35.0 4.0 39.0 53 23.708819661351306 29.0 4.0 35.0 4.0 38.0 54 23.352169981916816 29.0 4.0 35.0 4.0 38.0 55 23.082853855005755 29.0 4.0 35.0 4.0 38.0 56 22.701216505013974 29.0 4.0 35.0 4.0 38.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1 1 2.8658824480221057 1 2 4.891635728502898 1 3 4.8681872112012385 1 4 5.615993069082187 1 5 5.113516948718512 1 6 7.458422988243033 1 7 4.426321203102098 1 8 4.342676159278014 1 9 4.785953990410917 1 10 5.682675821561013 1 11 0.7200054087884169 1 12 -6.9947273860355885 1 13 -18.09359657929168 1 14 -5.623496988713246 1 15 2.649431562832156 1 16 -1.5227315992475638 1 17 -6.638245093307976 1 18 -4.660042495023784 1 19 -0.729061205873105 1 20 -1.2296936660624809 1 21 5.5744595361692575 1 22 6.682746370264692 1 23 6.287341511310199 1 24 6.387578083539019 1 25 5.615124656692792 1 26 5.986643745679427 1 27 4.75148768217268 1 28 5.860536131985725 1 29 6.184106888858182 1 30 6.78478533238064 1 31 4.8581698108644495 1 32 2.6644812707676486 1 33 1.9453813202814594 1 34 5.263122211442418 1 35 4.731709153869115 1 36 3.154079453321394 1 37 3.937112702010406 1 38 2.733223836466454 1 39 3.3127640608199336 1 40 -11.532377065659297 1 41 -0.21778821355948708 1 42 -2.5393371114276775 1 43 -9.317881296275697 1 44 -15.272233214178646 1 45 -5.3097086002461324 1 46 -6.080586604729529 1 47 -11.580506300241854 1 48 -6.340928254790947 1 49 -12.913241956829342 1 50 -5.177829564881041 1 51 0.1143586305675015 1 52 0.20747173998679713 1 53 0.022547830692129622 1 54 0.35590105807103356 1 55 0.9404284472836899 1 56 3.72210050625214 2 1 4.269351527961774 2 2 5.113355185516475 2 3 5.592169111653732 2 4 5.680849780997718 2 5 5.834482257919113 2 6 7.5270504392234265 2 7 5.405205064338901 2 8 5.3396595680261285 2 9 5.704506026610012 2 10 6.316159984456938 2 11 4.744892286616476 2 12 2.038455117735147 2 13 3.5165090014021345 2 14 5.908931367244524 2 15 6.869642724219787 2 16 5.663542910556359 2 17 8.180005283765933 2 18 6.039806675413622 2 19 6.760380724745293 2 20 6.482221869382464 2 21 7.39120161761722 2 22 7.8501671847443255 2 23 7.533193698338859 2 24 8.23674852094475 2 25 6.999740041308179 2 26 6.561304379163591 2 27 5.677581196501485 2 28 6.182557248124489 2 29 7.070984716912477 2 30 7.117364517901006 2 31 3.541427729416487 2 32 4.3447225980677935 2 33 5.757598514851598 2 34 5.020286615665643 2 35 5.252825292632313 2 36 7.222706904301788 2 37 6.971049353594115 2 38 7.3750036253050695 2 39 6.973397544982827 2 40 6.0264464637524675 2 41 10.516751756274601 2 42 10.347540716626618 2 43 8.912887934493536 2 44 -0.6990808763204264 2 45 9.469326090553263 2 46 8.625295748211649 2 47 4.786009536862217 2 48 9.147759528014483 2 49 1.5995785559911724 2 50 8.758822018829367 2 51 11.570618057415164 2 52 11.917124831992826 2 53 12.576092325413097 2 54 11.528600907241476 2 55 12.405737647887012 2 56 12.214559028122427 3 1 3.812941271551516 3 2 4.563904636065928 3 3 5.086674606159221 3 4 5.036161136309076 3 5 4.794189217626069 3 6 7.5651456773186645 3 7 3.7473296064634454 3 8 3.319146747513308 3 9 3.1220884441924284 3 10 4.7952808635778155 3 11 4.628408770132957 3 12 5.578381857661888 3 13 6.236655521548656 3 14 5.586587044900195 3 15 1.7956500502271169 3 16 5.873066720080168 3 17 6.858393562154209 3 18 7.884495320102268 3 19 5.802871567236135 3 20 5.23753322469382 3 21 5.087904914320518 3 22 7.417200151777298 3 23 5.776417141562302 3 24 7.211107495303722 3 25 5.729410370978506 3 26 5.497568115427324 3 27 5.489302808223094 3 28 5.229443695010936 3 29 5.730325376253141 3 30 5.750331550868037 3 31 4.844724432713189 3 32 4.398935052280255 3 33 4.023532580785659 3 34 4.397575992955023 3 35 4.996415036222057 3 36 5.632230713825592 3 37 5.858961441506207 3 38 5.9105347608361996 3 39 8.322848094433379 3 40 9.083589320895324 3 41 12.42590926543211 3 42 11.264024233110135 3 43 11.282118703724304 3 44 10.884069306829755 3 45 11.166761987989158 3 46 12.911010033925933 3 47 13.821174372027052 3 48 13.413693593948548 3 49 14.001043757456369 3 50 12.128052788060138 3 51 12.95889644569355 3 52 12.971337286205287 3 53 13.540194889515657 3 54 13.474388453029015 3 55 13.255554497703859 3 56 14.734705548268945 4 1 4.223467587340991 4 2 5.499994861629837 4 3 5.725772350520124 4 4 5.953454369391778 4 5 5.779151623641113 4 6 8.29898026378483 4 7 5.030036373380739 4 8 5.098094115934366 4 9 5.3138177675007014 4 10 6.018589134254505 4 11 6.119386213741983 4 12 7.616727722323546 4 13 7.4125953711727135 4 14 6.571549450915239 4 15 7.609183884813582 4 16 7.935472735117763 4 17 9.27042363734218 4 18 8.79351787649324 4 19 7.1780595371609515 4 20 6.886405405144949 4 21 7.229258297779161 4 22 8.91494451267955 4 23 7.641830675396886 4 24 7.711107495303722 4 25 7.088808867219107 4 26 7.000575634224315 4 27 6.575017093937383 4 28 5.21966925892071 4 29 6.8807013161027655 4 30 6.868376663649993 4 31 5.571792101886121 4 32 4.765100465814083 4 33 4.83857017477062 4 34 6.15321509069938 4 35 5.970099246748372 4 36 6.310426202547397 4 37 7.543923847521246 4 38 7.860158820986577 4 39 7.086757868869466 4 40 3.1843412005945737 4 41 12.68004460377798 4 42 11.26252047371164 4 43 11.050539756355885 4 44 12.514144494799684 4 45 10.393077777462846 4 46 11.388453642948491 4 47 14.162527755485698 4 48 13.465573293196666 4 49 13.086758043170658 4 50 13.659631735428555 4 51 13.410024265242424 4 52 12.91645006816017 4 53 13.455984363199871 4 54 13.055591460547816 4 55 12.943524422515889 4 56 12.979066450524588 5 1 3.742671001281245 5 2 5.475101547262838 5 3 5.326056845541466 5 4 6.186740286888224 5 5 5.96407338751024 5 6 8.058581970754954 5 7 5.337290996424834 5 8 5.113741342107907 5 9 5.280389602493585 5 10 5.218446886743841 5 11 5.676285218009404 5 12 6.983015062295095 5 13 7.249010733903866 5 14 6.675390133703285 5 15 7.87518672976379 5 16 7.168047415060862 5 17 8.25762136138201 5 18 9.155537791144738 5 19 8.185883150247722 5 20 6.60973399689459 5 21 6.220723447139051 5 22 7.712953047530188 5 23 7.10769127283643 5 24 6.96786425206048 5 25 6.344854386422519 5 26 5.220348038207252 5 27 5.764206283126569 5 28 5.475003540570782 5 29 6.950402596330356 5 30 6.8491732497097395 5 31 5.279473467462228 5 32 5.478471731816928 5 33 6.194188951442033 5 34 5.72576132114035 5 35 4.9153339551409765 5 36 6.781265462860347 5 37 6.877494260039025 5 38 7.290457540758986 5 39 6.908948480533763 5 40 8.702122139428145 5 41 10.987299226822074 5 42 10.064796433882336 5 43 10.709145730751327 5 44 11.03619285895331 5 45 9.907302528529701 5 46 10.78745791037381 5 47 11.505344256196935 5 48 11.42218780244276 5 49 11.41108236749498 5 50 11.068593328600677 5 51 9.970479457276564 5 52 8.834657749525746 5 53 10.167221916542687 5 54 8.692149070789636 5 55 9.294164536313897 5 56 8.163276976840375 6 1 3.796725055335301 6 2 5.339966412127701 6 3 5.109840629325248 6 4 5.78133488148282 6 5 5.585695009131861 6 6 8.058581970754954 6 7 5.310263969397802 6 8 4.924552152918714 6 9 4.874984197088182 6 10 5.461690129987083 6 11 5.433041974766162 6 12 7.361393440673474 6 13 7.681443166336301 6 14 5.999714458027615 6 15 7.794105648682709 6 16 7.113993361006806 6 17 7.960324064084713 6 18 9.452835088442036 6 19 7.158856123220698 6 20 6.312436699597292 6 21 7.1666693930849945 6 22 7.226466561043704 6 23 6.972556137701293 6 24 8.130026414222641 6 25 5.885394926963066 6 26 5.896023713882922 6 27 5.331773850694141 6 28 5.664192729759975 6 29 6.085537731465493 6 30 6.443767844304329 6 31 4.738932926921688 6 32 5.721714975060177 6 33 5.302297059550138 6 34 5.536572131951157 6 35 5.482901522708541 6 36 5.483968165563049 6 37 5.526142908687671 6 38 6.182349432650874 6 39 5.719759291344577 6 40 2.4318518691578745 6 41 10.798110037632881 6 42 9.037769406855311 6 43 11.303740325345924 6 44 10.711868534628987 6 45 9.069464690691863 6 46 11.733403856319754 6 47 12.072911823764507 6 48 11.611376991631946 6 49 12.546217502630117 6 50 10.31183657184392 6 51 11.48399297079008 6 52 10.213036127904125 6 53 10.194248943569711 6 54 10.665122043762608 6 55 10.996867239016602 6 56 9.974087787651186 7 1 4.407678113656779 7 2 5.710521177419309 7 3 5.883667087362234 7 4 6.006085948339148 7 5 5.91073057100953 7 6 7.798980263784831 7 7 5.003720583907054 7 8 5.650725694881729 7 9 5.866449346448071 7 10 6.465957555307142 7 11 5.987807266373558 7 12 7.853569827586703 7 13 7.544174318541138 7 14 6.361023135125759 7 15 8.082868095339897 7 16 7.277577998275653 7 17 8.71779205839481 7 18 9.00404419228272 7 19 8.020164800318842 7 20 7.044300141987051 7 21 8.045047771463373 7 22 8.546523460047968 7 23 6.904988570133728 7 24 7.237423284777407 7 25 6.85196676195595 7 26 6.658470371066425 7 27 6.285543409726856 7 28 7.061774522078608 7 29 6.90701710557645 7 30 7.236797716281568 7 31 4.992844733465073 7 32 5.05457415002461 7 33 5.522780701086415 7 34 6.10058351175201 7 35 6.15430977306416 7 36 7.310426202547397 7 37 8.043923847521246 7 38 8.12331671572342 7 39 8.507810500448418 7 40 9.34223593743668 7 41 11.416886709041137 7 42 11.920415210553749 7 43 12.392645019513775 7 44 13.645723442168102 7 45 11.577288303778634 7 46 15.177927327159018 7 47 16.08358038706465 7 48 14.518204872144036 7 49 14.060442253696973 7 50 15.107000156481192 7 51 14.515287423137163 7 52 14.811186910265437 7 53 15.087563310568289 7 54 13.66085461844255 7 55 15.101419159357992 7 56 13.294855924208793 8 1 3.9835295068456347 8 2 4.803400434385253 8 3 5.279951917083594 8 4 5.115993069082187 8 5 5.142928713424389 8 6 7.81136416471362 8 7 4.308674144278569 8 8 4.460323218101543 8 9 4.609483402175627 8 10 5.594440527443361 8 11 5.808240702906069 8 12 7.005272613964408 8 13 7.053462244237728 8 14 6.552973599522044 8 15 7.472960974596859 8 16 6.594915459575965 8 17 7.65587255375085 8 18 8.281133975564451 8 19 7.123879970597482 8 20 6.211482804525751 8 21 6.868577183228076 8 22 7.565099311441163 8 23 6.199106217192554 8 24 7.711107495303722 8 25 6.67394818610456 8 26 6.574879039797072 8 27 3.810311211584441 8 28 3.6840655437504353 8 29 5.331165712387595 8 30 6.196550038262995 8 31 4.8287580461585655 8 32 4.517422447238236 8 33 4.298322496752046 8 34 4.057239858501237 8 35 4.702297389163231 8 36 5.007020629791981 8 37 4.201818584363348 8 38 4.733223836466458 8 39 5.224528766702289 8 40 1.114681757870116 8 41 8.370447080558165 8 42 6.6959570062193805 8 43 6.476236350783125 8 44 8.168943256409587 8 45 7.719703164459748 8 46 11.537060454094004 8 47 10.948905464464026 8 48 9.894365862856112 8 49 10.792640396111835 8 50 10.410405729236608 8 51 11.70259392468515 8 52 9.589824681163268 8 53 10.199018418927423 8 54 9.414724587482798 8 55 10.79336962375428 8 56 9.310335800369788 9 1 3.5754412715515187 9 2 4.827297493208782 9 3 5.171496034730652 9 4 5.103125422023361 9 5 5.249546360483215 9 6 6.849967105890091 9 7 5.005365320749156 9 8 4.894146747513311 9 9 4.706909872763859 9 10 5.342602292149245 9 11 5.764123055847243 9 12 5.782846143376176 9 13 7.308976950120083 9 14 6.416944187757341 9 15 8.204578621655685 9 16 6.955209577223023 9 17 8.462857847868495 9 18 9.08628103438798 9 19 6.669835852950424 9 20 6.935747510408106 9 21 6.3262977714633735 9 22 7.419878723205866 9 23 6.686238570133728 9 24 6.711107495303722 9 25 6.458874656692792 9 26 5.306496686855898 9 27 5.043767093937383 9 28 4.39730083786808 9 29 4.364253947681711 9 30 5.641402979439466 9 31 2.4813315755703336 9 32 3.6516136237088226 9 33 4.024425437928517 9 34 3.5002545643835923 9 35 3.496415036222057 9 36 4.951873570968452 9 37 4.733068584363348 9 38 5.470356189407632 9 39 4.509455237290521 9 40 2.6826964637524675 9 41 7.749123551146397 9 42 8.015809947395852 9 43 8.963368703724303 9 44 9.698355021115471 9 45 8.054261987989161 9 46 10.031545748211649 9 47 10.29081722916991 9 48 11.445836451091406 9 49 11.274258043170658 9 50 9.934302788060137 9 51 9.454432159979266 9 52 11.817765857633855 9 53 10.046444889515659 9 54 10.752959881600447 9 55 9.344840211989574 9 56 8.288276976840375 10 1 3.068496827107076 10 2 5.032158604319896 10 3 4.959690479175094 10 4 5.321875422023361 10 5 4.131490804927658 10 6 7.499272661445648 10 7 4.4671708763047135 10 8 4.102480080846647 10 9 4.745104317208302 10 10 5.679407847704802 10 11 4.736345278069464 10 12 6.702985032265062 10 13 7.437449172342305 10 14 6.701666409979563 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5.4081031775877975 116 4 6.071875422023361 116 5 5.794189217626069 116 6 6.693717105890091 116 7 4.661615320749156 116 8 4.1977181760847415 116 9 5.157802729906713 116 10 5.5167094350063905 116 11 4.264123055847243 116 12 4.528381857661891 116 13 5.393798378691514 116 14 5.515158473471626 116 15 -3.090064235487173 116 16 4.580209577223023 116 17 6.315536419297064 116 18 6.184495320102265 116 19 4.710014424378993 116 20 5.408961796122391 116 21 6.437904914320519 116 22 5.817200151777296 116 23 5.9764171415622975 116 24 6.425393209589437 116 25 6.365124656692792 116 26 6.5118538297130435 116 27 6.110731379651668 116 28 4.9865865521537955 116 29 6.837468233395995 116 30 5.828902979439466 116 31 4.2804387184274795 116 32 3.7632207665659685 116 33 5.894961152214233 116 34 5.040433135812162 116 35 5.817843607650627 116 36 6.675087856682737 116 37 5.844675727220494 116 38 7.8319633322647775 116 39 8.73713380871909 116 40 11.169303606609613 116 41 7.275909265432112 116 42 4.0783099473958515 116 43 0.46783298943858753 116 44 1.8769264496868985 116 45 6.259619130846303 116 46 1.6610100339259333 116 47 -5.985968485115803 116 48 0.035122165377121206 116 49 -6.7703848139722 116 50 1.528052788060137 116 51 4.923182159979266 116 52 4.621337286205282 116 53 5.318766318087086 116 54 5.038674167314731 116 55 5.898411640561001 116 56 7.520419833983233 117 1 2.5392570610252037 117 2 3.526310651103522 117 3 3.883667087362234 117 4 4.532401737812833 117 5 4.4633621499569 117 6 5.983190790100618 117 7 3.6089837418017865 117 8 4.098094115934366 117 9 4.129607241184914 117 10 3.6238522921492446 117 11 -0.06482431257381194 117 12 -11.09379859346593 117 13 -11.587404628827287 117 14 -3.7968716017163437 117 15 -16.180289799396945 117 16 -10.617158843829607 117 17 0.007265742605337522 117 18 -3.65385054455939 117 19 -1.111414147049576 117 20 -2.1399103843287364 117 21 5.281889876726531 117 22 4.599155038995338 117 23 4.089199096449516 117 24 5.526896968987934 117 25 4.720387814587532 117 26 4.13215458159274 117 27 4.522385514990013 117 28 3.930195574710183 117 29 5.038596052944868 117 30 5.421008242597363 117 31 3.4928447334650734 117 32 4.10720572897198 117 33 4.89120175371799 117 34 3.4953203538572772 117 35 4.470099246748372 117 36 5.836741992021082 117 37 7.307081742258088 117 38 6.5180535578286865 117 39 7.560442079395781 117 40 10.552762253226152 117 41 6.258991972199027 117 42 2.1046257368695365 117 43 -5.107354980486225 117 44 -5.3279607683582135 117 45 4.893077777462846 117 46 -1.585230567577824 117 47 -0.5216827708300897 117 48 -1.5081109173296454 117 49 -2.8606103778819723 117 50 -3.1035261593082843 117 51 2.51528742313716 117 52 3.1532921734233277 117 53 3.245458047410395 117 54 4.608223039495183 117 55 4.338261264621153 117 56 6.1106453978930055 118 1 2.1708360083936213 118 2 3.5789422300508846 118 3 3.5678776136780215 118 4 3.9008227904444155 118 5 3.9370463604832153 118 6 6.088453947995351 118 7 3.293194268117574 118 8 3.045462536986996 118 9 3.550659872763859 118 10 4.09753650267556 118 11 -1.0648243125738084 118 12 -10.725377540834351 118 13 -12.692667786722023 118 14 -9.90213475961108 118 15 -10.232921378344315 118 16 -11.143474633303292 118 17 -2.255892152131505 118 18 -9.285429491927811 118 19 -2.637729936523261 118 20 -2.929384068539264 118 21 2.387153034621271 118 22 4.704418196890078 118 23 3.1418306753968857 118 24 4.421633811093194 118 25 2.29933518300858 118 26 5.13215458159274 118 27 4.153964462358438 118 28 3.82493241681545 118 29 4.196490789786971 118 30 4.263113505755253 118 31 2.229686838728231 118 32 3.5282583605509252 118 33 3.83857017477062 118 34 4.60058351175201 118 35 3.680625562537845 118 36 4.889373570968452 118 37 5.254450163310718 118 38 3.254895663091844 118 39 3.0341262899220993 118 40 6.605393832173522 118 41 3.1010972353569244 118 42 3.1046257368695365 118 43 -1.4757760331178034 118 44 -0.2753291894108436 118 45 3.5772883037786336 118 46 0.2042431166326999 118 47 -1.4690511918827198 118 48 1.070836451091406 118 49 -6.96587353577671 118 50 1.8964738406917157 118 51 4.988971633663475 118 52 1.8901342786864852 118 53 1.1928264684630285 118 54 2.9240125131793953 118 55 3.2329981067264164 118 56 4.268540134735112 119 1 2.9129412715515173 119 2 4.271047493208783 119 3 4.315246034730649 119 4 5.071875422023361 119 5 3.837046360483214 119 6 6.793717105890092 119 7 3.8616153207491593 119 8 4.1691467475133095 119 9 2.550659872763859 119 10 3.3738522921492446 119 11 0.3141230558472401 119 12 -6.585903856623823 119 13 -4.684773049879915 119 14 -7.62055581224266 119 15 -7.932921378344314 119 16 -11.369790422776976 119 17 -2.8558921521315064 119 18 -6.301218965612019 119 19 -4.161414147049577 119 20 -4.326752489591893 119 21 1.195047771463372 119 22 1.838628723205865 119 23 1.7049885701337253 119 24 1.2111074953037217 119 25 1.2151246566927938 119 26 2.3689966868558976 119 27 2.1750170939373845 119 28 1.972300837868083 119 29 3.0017539476817134 119 30 3.2789029794394686 119 31 1.6875815755703307 119 32 0.970363623708824 119 33 2.38067543792852 119 34 2.419004564383595 119 35 2.746415036222057 119 36 3.0393735709684506 119 37 3.701818584363348 119 38 3.6391061894076344 119 39 3.4657052372905213 119 40 6.5264464637524675 119 41 3.6616235511463984 119 42 3.72830994739585 119 43 -3.7678812962756965 119 44 -3.3016449788845286 119 45 3.3167619879891603 119 46 -2.424704251788352 119 47 -4.471682770830089 119 48 -7.029163548908594 119 49 -2.463241956829343 119 50 -0.5219472119398638 119 51 6.473182159979267 119 52 5.342765857633854 119 53 3.540194889515657 119 54 4.752959881600447 119 55 5.1198402119895725 119 56 8.513276976840377 120 1 2.562941271551516 120 2 3.5210474932087834 120 3 3.9652460347306544 120 4 4.5218754220233635 120 5 4.787046360483217 120 6 6.843717105890089 120 7 3.9616153207491536 120 8 3.9191467475133095 120 9 4.500659872763862 120 10 4.023852292149243 120 11 1.8641230558472444 120 12 -6.635903856623823 120 13 -0.634773049879918 120 14 0.4794441877573412 120 15 1.7670786216556849 120 16 -6.469790422776978 120 17 0.04410784786849575 120 18 -6.60121896561202 120 19 -2.5114141470495746 120 20 -1.0767524895918932 120 21 3.0950477714633706 120 22 4.888628723205866 120 23 4.854988570133731 120 24 4.661107495303725 120 25 4.265124656692791 120 26 6.218996686855899 120 27 5.325017093937383 120 28 4.02230083786808 120 29 6.401753947681712 120 30 7.178902979439467 120 31 4.237581575570335 120 32 3.5703636237088254 120 33 4.180675437928517 120 34 4.519004564383589 120 35 3.0964150362220586 120 36 4.789373570968451 120 37 4.651818584363351 120 38 5.689106189407632 120 39 6.415705237290521 120 40 6.376446463752469 120 41 -6.638376448853602 120 42 -2.52169005260415 120 43 -10.067881296275697 120 44 -14.301644978884529 120 45 -4.48323801201084 120 46 -9.82470425178835 120 47 -8.77168277083009 120 48 -8.779163548908594 120 49 -13.463241956829343 120 50 -6.521947211939864 120 51 -0.076817840020734 120 52 0.6427658576338544 120 53 1.340194889515658 120 54 1.5029598816004466 120 55 1.3198402119895754 120 56 5.213276976840376 >>END_MODULE >>Per sequence quality scores pass #Quality Count 4 904.0 5 15.0 6 68.0 7 57.0 8 51.0 9 43.0 10 51.0 11 45.0 12 58.0 13 52.0 14 85.0 15 99.0 16 121.0 17 157.0 18 206.0 19 275.0 20 354.0 21 464.0 22 516.0 23 609.0 24 679.0 25 762.0 26 784.0 27 820.0 28 866.0 29 920.0 30 985.0 31 1102.0 32 1169.0 33 1373.0 34 1536.0 35 1652.0 36 1825.0 37 2006.0 38 1964.0 39 1658.0 40 1.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 0.0 0.0 0.0 100.0 2 0.0 0.0 100.0 0.0 3 0.0 0.0 0.0 100.0 4 100.0 0.0 0.0 0.0 5 0.0 0.0 0.0 100.0 6 0.0 100.0 0.0 0.0 7 99.6465559756699 0.2465888541837909 0.045207956600361664 0.061647213545947724 8 99.58901857636035 0.22192996876541182 0.06986684201874074 0.11918461285549892 9 98.97665625513726 0.39043235245766894 0.3657734670392898 0.26713792536577347 10 59.859444353115244 20.783330593457176 10.175900049317772 9.181325004109814 11 39.219957257931945 24.227354923557456 16.611869143514713 19.940818674995892 12 31.86749958901858 18.448956107183957 21.465559756698998 28.217984547098474 13 24.219135295084662 15.156994903830348 24.708203189215848 35.91566661186915 14 23.035508795002464 24.958901857636036 26.923393062633572 25.08219628472793 15 29.49167939876946 20.027253582194326 23.888177726390552 26.592889292645662 16 31.289579735175586 24.134386051484498 22.468953038901226 22.107081174438687 17 27.783943766185722 19.735273564352365 29.81871994080651 22.662062728655403 18 25.435640309058034 19.340785796482 27.433010027946736 27.790563866513235 19 28.847498046955305 22.54841495004317 21.101106040047696 27.502980962953828 20 28.811885262768016 22.042059344005928 25.165644676735667 23.98041071649039 21 25.690448791714616 22.06148282097649 25.300016439256943 26.948051948051948 22 22.706723656090745 19.332566168009205 30.338648693079072 27.622061482820975 23 22.8793358540194 23.51635706066086 25.727437119842183 27.876869965477564 24 22.23911881961284 22.329538448892357 26.06962311454523 29.36171961694957 25 26.12412659268393 24.911631730374022 24.99383477188656 23.970406905055487 26 29.32516346588806 24.29164781839865 22.35884360735288 24.024345108360407 27 23.657152257958376 25.0390721395081 20.88097392448795 30.42280167804557 28 25.680230168516232 24.730785039046445 23.205918618988903 26.383066173448416 29 31.81574939282921 23.480014819083685 19.61058741201169 25.09364837607541 30 28.058559855245303 21.421227947526422 26.816630340913765 23.70358185631451 31 30.443183686893605 25.427561256372304 21.7069560927479 22.422298963986186 32 33.27578410819254 23.13478850659761 21.663172606568835 21.926254778641017 33 30.569159224659455 23.223177908555908 21.400057615539733 24.807605251244908 34 25.457988555432053 23.90185665472809 28.632003622740932 22.008151167098923 35 24.73967979585957 24.40630530518171 30.444087747458532 20.409927151500185 36 27.548209366391184 24.892068582706305 24.637144854241193 22.922577196661322 37 27.677615872231826 22.198896846958096 27.56647731950276 22.55700996130732 38 29.647020058486756 26.356110218707524 22.710984801680464 21.285884921125252 39 28.863093768008564 23.86597513789413 20.395982547130977 26.874948546966333 40 22.631731686082666 18.80933626300734 24.124207122424444 34.43472492848555 41 20.77619878666999 16.143106457242585 21.528297182747444 41.55239757333998 42 24.844413304208054 24.988665869842972 22.359147673412192 27.807773152536786 43 21.85182132849843 19.758529751112576 27.39409922531729 30.995549695071702 44 22.954676793527614 22.797820523404607 23.949475769834063 30.298026913233716 45 26.399242205839958 22.68028499649932 26.745191713685596 24.175281083975126 46 22.299191819231403 26.612238165924463 24.418604651162788 26.669965363681346 47 19.364699078854972 23.763889462596556 24.25131149572473 32.620099962823744 48 25.001028002796165 22.41868497882314 25.82343023973025 26.75685677865044 49 21.825445858560897 23.22171423864245 24.206104040528853 30.746735862267805 50 24.4234887168506 23.599901169494316 26.774831164552793 25.201778949102287 51 23.089290131090774 24.449666089537473 27.710446038420315 24.750597740951438 52 22.476119894598156 25.716403162055336 26.988636363636363 24.818840579710145 53 23.39706609526949 23.47535849678589 27.649579693423437 25.477995714521178 54 23.795788866455148 23.058222423668052 28.09757303555977 25.04841567431703 55 21.70437219268966 23.365063666707876 28.43779618411835 26.492767956484116 56 23.35928809788654 21.810241832488774 28.974580809953448 25.855889259671237 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 1.0 7 2.0 8 2.0 9 2.0 10 1.5 11 1.0 12 3.0 13 10.0 14 15.0 15 19.5 16 24.0 17 24.5 18 25.0 19 22.0 20 19.0 21 20.5 22 20.0 23 18.0 24 29.5 25 41.0 26 43.5 27 46.0 28 45.0 29 55.5 30 67.0 31 88.0 32 109.0 33 116.5 34 124.0 35 157.0 36 190.0 37 223.5 38 285.0 39 313.0 40 336.5 41 360.0 42 421.5 43 483.0 44 546.5 45 610.0 46 816.5 47 1328.5 48 1634.0 49 1670.0 50 1706.0 51 1522.0 52 1338.0 53 1353.0 54 1396.0 55 1424.0 56 1563.0 57 1702.0 58 1580.5 59 1459.0 60 1549.0 61 1639.0 62 1722.0 63 1679.0 64 1553.0 65 1419.0 66 1285.0 67 1199.0 68 1113.0 69 905.5 70 698.0 71 652.0 72 507.0 73 408.0 74 380.5 75 353.0 76 267.0 77 181.0 78 128.5 79 70.0 80 64.0 81 71.5 82 79.0 83 54.5 84 30.0 85 20.0 86 10.0 87 5.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.5 95 1.0 96 0.5 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.47262863718559917 16 0.05753739930955121 17 0.020549071181982577 18 0.0 19 0.045207956600361664 20 0.13562386980108498 21 0.0 22 0.0 23 0.0 24 0.004109814236396515 25 0.00821962847279303 26 0.061647213545947724 27 0.07397665625513726 28 0.00821962847279303 29 0.16028275521946408 30 0.061647213545947724 31 0.03287851389117212 32 0.020549071181982577 33 0.13562386980108498 34 0.16850238369225712 35 0.14384349827387802 36 0.045207956600361664 37 0.15617294098306755 38 0.21782015452901526 39 0.15617294098306755 40 0.8671708038796646 41 1.093210586881473 42 0.28357718231135953 43 0.26302811112937696 44 0.43564030905803053 45 0.20960052605622226 46 0.32878513891172123 47 0.5055071510767714 48 0.05342758507315469 49 0.21782015452901526 50 0.1972710833470327 51 0.3041262534933421 52 0.18083182640144665 53 0.26302811112937696 54 0.2589182968929804 55 0.26713792536577347 56 0.2424790399473944 >>END_MODULE >>Sequence Length Distribution pass #Length Count 56 24332.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 73.84925201380898 #Duplication Level Percentage of deduplicated Percentage of total 1 88.38555289665535 65.27206970244946 2 7.034336913573376 10.38961038961039 3 1.9255384272914462 4.265987177379582 4 0.8403361344537815 2.482327798783495 5 0.46190661695141627 1.7055729081045536 6 0.2559964383104235 1.1343087292454381 7 0.20034503867772274 1.0356731875719216 8 0.15025877900829207 0.8877198750616473 9 0.08904223941232123 0.5918132500410981 >10 0.5954699760698982 7.92372184777248 >50 0.044521119706160614 2.396021699819168 >100 0.01669541988981023 1.915173434160776 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source CTCGCAGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAG 197 0.8096334045701135 No Hit CTCGCAGGGTCACAGATCCTCTTCTGAGATGAGTTTTTGTTCAATCCGCCCAATCT 154 0.6329113924050633 No Hit CTCGCAGGGGGGCGCTGACCCCCCTTCCCGGGGGGGGATGCGTGCATTTATCAGAT 115 0.47262863718559917 No Hit CTCGCAGGGTCACAGATCCTCTTCTGAAGGAGATTGGGCGGATTGAACAAAAACTC 98 0.4027617951668585 No Hit CTCGCAGGGAACTGTGGTAATTCTAGAGCTAATACATGCCGACGGGCGCTGACCCC 98 0.4027617951668585 No Hit CTCGCAGGGGGTTCCTTTGGTCGCTCGCTCCTCTCCTACTTGGATAACTGTGGTAA 80 0.32878513891172123 No Hit CTCGCAGGGAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGTC 69 0.28357718231135953 No Hit CTCGCAGGGCTCCTACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCGAC 67 0.27535755383856647 No Hit CTCGCAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTC 60 0.2465888541837909 No Hit CTCGCAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC 58 0.23836922571099783 No Hit CTCGCAGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTCGTCTCAAAGATTAAG 53 0.21782015452901526 No Hit CTCGCAGGGGTGGCGGCGACGACCCATTCGAACGTCTGCCCTATCAACTTTCGATG 46 0.1890514548742397 No Hit CTCGCAGGGGGATAACTGTGGTAATTCTAGAGCTAATACATGCCGACGGGCGCTGA 41 0.16850238369225712 No Hit CTCGCAGGGAGTTATGGTTCCTTTGGTCGCTCGCTCCTCTCCTACTTGGATAACTG 38 0.15617294098306755 No Hit CTCGCAGGGGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCCATTCGAACGTC 36 0.14795331251027452 No Hit CTCGCAGGGGCTCGCTCCTCTCCTACTTGGATAACTGTGGTAATTCTAGAGCTAAT 36 0.14795331251027452 No Hit CTCGCAGGGGGCTACCACATCCAAGGAAGGCAGCAGGCGCGCAAATTACCCACTCC 35 0.14384349827387802 No Hit CTCGCAGGGGGTGAGCTCCCTCCCGGCTCCGGCCGGGGGTCGGGCGCCGGCGGCTT 35 0.14384349827387802 No Hit CTCGCAGGGTACCTGGTTGATCCTGCCAGTAGCATATGCCTGTCTCAAAGATTAAG 33 0.13562386980108498 No Hit CTCGCAGGGAACTGTGGTAATTCTAGAGCTAATACATGCCCACGGGCGCTGACCCC 31 0.12740424132829195 No Hit CTCGCAGGGGGGTGACGGGGAATCAGGGTTCGATTCCGGAGAGGGAGCCTGAGAAA 30 0.12329442709189545 No Hit CTCGCAGGGTCACAGATCCTCTTCTGAGATGAGTTTTTGTCCAATCCGCCCAATCT 30 0.12329442709189545 No Hit CTCGCAGGGGGTGACTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGC 30 0.12329442709189545 No Hit CTCGCAGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTT 29 0.11918461285549892 No Hit CTCGCAGGGAAGCCATGCATGTCTAAGTACGCACGGCCGGTACAGTGAAACTGCGA 29 0.11918461285549892 No Hit CTCGCAGGGATCAGATCAAAACCAACCCGGTGAGCTCCCTCCCGGCTCCGGCCGGG 28 0.11507479861910241 No Hit CTCGCAGGGGGAGAGGGAGCCTGAGAAACGGCTACCACATCCAAGGAAGGCAGCAG 28 0.11507479861910241 No Hit CTCGCAGGGTCACAGATCCTCTTCTGAAGGAGATTGGGCGCATTGAACAAAAACTC 28 0.11507479861910241 No Hit CTCGCAGGGGGCGGCGACGACCCATTCGAACGTCTGCCCTATCAACTTTCGATGGT 27 0.11096498438270591 No Hit CTCGCAGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCCAAGATTAAG 27 0.11096498438270591 No Hit CTCGCAGGGGGTTCCTTTGGTCGCTCGCTCCTCTCCTACTCGGATAACTGTGGTAA 27 0.11096498438270591 No Hit CTCGCAGGGCTCCTACTTGGATAACTGTGGTAATTCTAGACCTAATACATGCCGAC 25 0.10274535590991288 No Hit CTCGCAGGGATCCAAGGAAGGCAGCAGGCGCGCAAATTACCCACTCCCGACCCGGG 25 0.10274535590991288 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.004109814236396515 0.0 0.0 0.0 0.0 11 0.004109814236396515 0.0 0.0 0.0 0.0 12 0.004109814236396515 0.0 0.0 0.0 0.0 13 0.004109814236396515 0.0 0.0 0.0 0.0 14 0.02465888541837909 0.0 0.0 0.0 0.0 15 0.028768699654775604 0.0 0.0 0.0 0.0 16 0.028768699654775604 0.0 0.0 0.0 0.0 17 0.028768699654775604 0.0 0.0 0.0 0.0 18 0.028768699654775604 0.0 0.0 0.0 0.0 19 0.03287851389117212 0.0 0.0 0.0 0.0 20 0.03287851389117212 0.0 0.0 0.0 0.0 21 0.03698832812756864 0.0 0.0 0.0 0.0 22 0.03698832812756864 0.0 0.0 0.0 0.0 23 0.03698832812756864 0.0 0.0 0.0 0.0 24 0.03698832812756864 0.0 0.0 0.0 0.0 25 0.03698832812756864 0.0 0.0 0.0 0.0 26 0.03698832812756864 0.0 0.0 0.0 0.0 27 0.04109814236396515 0.0 0.0 0.0 0.0 28 0.045207956600361664 0.0 0.0 0.0 0.0 29 0.05342758507315469 0.0 0.0 0.0 0.0 30 0.05342758507315469 0.0 0.0 0.0 0.0 31 0.061647213545947724 0.0 0.0 0.0 0.0 32 0.061647213545947724 0.0 0.0 0.0 0.0 33 0.061647213545947724 0.0 0.0 0.0 0.0 34 0.061647213545947724 0.0 0.0 0.0 0.0 35 0.061647213545947724 0.0 0.0 0.0 0.0 36 0.061647213545947724 0.0 0.0 0.0 0.0 37 0.061647213545947724 0.0 0.0 0.0 0.0 38 0.06575702778234424 0.0 0.0 0.0 0.0 39 0.06575702778234424 0.0 0.0 0.0 0.0 40 0.06575702778234424 0.0 0.0 0.0 0.0 41 0.06575702778234424 0.0 0.0 0.0 0.0 42 0.06986684201874076 0.0 0.0 0.0 0.0 43 0.07808647049153378 0.0 0.0 0.0 0.0 44 0.08630609896432681 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CCATGCC 15 0.008137336 50.299377 47 CCCGCGG 15 0.008137336 50.299377 47 TCCACTC 15 0.008137336 50.299377 43 CAAGGCA 15 0.008137336 50.299377 15 CACATGG 15 0.008272314 50.0911 40 GACGGCG 15 0.008272314 50.0911 13 ATGGAGG 15 0.008272314 50.0911 36 CGACGGC 15 0.008272314 50.0911 12 GAGCATA 15 0.008340424 49.987602 9 TTAAGAG 15 0.008408951 49.884537 33 TATCAGA 25 2.0549845E-5 49.884537 49 GTTAAGA 15 0.008408951 49.884537 32 AGGGGTT 50 7.2759576E-12 49.781895 6 AGGGGTA 25 2.080304E-5 49.781895 6 AGGGTCA 85 0.0 49.781895 6 AGGGCAG 25 2.080304E-5 49.781895 6 AGGGTAC 50 7.2759576E-12 49.781895 6 AGGGATT 60 0.0 49.781895 6 AGGGATC 25 2.080304E-5 49.781895 6 AGGGAGG 25 2.080304E-5 49.781895 6 >>END_MODULE