FastQCFastQC Report
Fri 17 Jun 2016
SRR1526169_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526169_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1488
Sequences flagged as poor quality0
Sequence length40
%GC52

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCGCAGGGATGAGATGGACTTAAAGGAATTGACGGGTAC533.5618279569892475No Hit
CTCGCAGGGATGAGATGGACTTAAATGAATTGACGGGTAC473.1586021505376345No Hit
CTCGCAGGGATGAGATGGCCTTAAAGGAATTGACGGGTAC362.4193548387096775No Hit
CTCGCAGGGATGAGATGGCCTTAAATGAATTGACGGGTAC302.0161290322580645No Hit
CTCGCAGGGATGAGATGGACTCAAAGGAATTGACGGGTAC211.411290322580645No Hit
CTCGCAGGGATGAGATGGACTCAAATGAATTGACGGGTAC191.2768817204301075No Hit
CTCGCAGGGATGAGATGGCCTCAAAGGAATTGACGGGTAC130.8736559139784945No Hit
CTCGCAGGGATGAGATGGCCTCAAATGAATTGACGGGTAC120.8064516129032258No Hit
CTCGCAGGGATGAGCTGGACTTAAAGGAATTGACGGGTAC100.6720430107526881No Hit
CTCGCAGGGATGAGATGGACTTAAATGAATTGACCGGTAC100.6720430107526881No Hit
CTCGCAGGGATGAGATGGACTCAAAGGAATTGACCGGTAC80.5376344086021506No Hit
CTCGCAGGGATGAGATGGACTTAACGGAATTGACGGGTAC80.5376344086021506No Hit
CTCGCAGGGATGAGATGGACTTAAAGGAATTGACCGGTAC70.47043010752688175No Hit
CTCGCAGGGATGAGATGGACTTACATGAATTGACGGGTAC70.47043010752688175No Hit
CTCGCAGGGATGAGATGGACTTAAAGGAATTGACGGGTCC50.33602150537634407No Hit
CTCGCAGGGATGAGATGGCCTTACAGGAATTGACGGGTAC50.33602150537634407No Hit
CTCGCAGGGATGAGATGGACTTACATGAATTGACCGGTAC50.33602150537634407No Hit
CTCGCAGGGATGAGATGGACTTAAATGAATTGCCGGGTAC40.2688172043010753No Hit
CTCGCAGGGATGAGCTGGACTTAAATGAATTGACGGGTAC40.2688172043010753No Hit
CTCGCAGGGATGAGATGGACTCAACGGAATTGACGGGTAC40.2688172043010753No Hit
CTCGCAGGGATGAGATGGACTTAACTGAATTGACGGGTAC40.2688172043010753No Hit
CTCGCAGGGATGAGATGGACTCCAATGAATTGACCGGTAC40.2688172043010753No Hit
CTCGCAGGGATGAGATGGACTCACAGGAATTGACGGGTAC40.2688172043010753No Hit
CTCGCAGGGGCTGGTTGTGGGGGTGGGGGAGATGTTTTAT30.20161290322580644No Hit
CTCGCAGGGATGAGATGGACTTAAAGGAATTGACGGATAC30.20161290322580644No Hit
CTCGCAGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCG30.20161290322580644No Hit
CTCGCAGGGATGAGATGGACTCCAATGAATTGACGGGTAC30.20161290322580644No Hit
CTCGCAGGGGGGAATTCGTGGAGAAAGAAATGGCTCCCCC30.20161290322580644No Hit
CTCGCAGGGATGAGATGGCCTTAAAGGAATTGACGGGTCC30.20161290322580644No Hit
CTCGCAGGGATGAGATGGCCTTAAATGACTTGACGGGTAC30.20161290322580644No Hit
CTCGCAGGGATGAGATGGCCTTCAATGAATTGACCGGTAC30.20161290322580644No Hit
CTCGCAGGGATGAGATGGCCTTAAATGAATTGCCGGGTAC30.20161290322580644No Hit
CTCGCAGGGATGAGATGGACTTAAATGAATTGACGGGTCC30.20161290322580644No Hit
CTCGCAGGGATGAGATGGACTCAACTGAATTGACGGGTAC30.20161290322580644No Hit
CTCGCAGGGATGAGATGGACTTACAGGAATTGACGGGTAC30.20161290322580644No Hit
CTCGCAGGGATGAGATGGCCTTAAAGGACTTGACGGGTAC30.20161290322580644No Hit
CTCGCAGGGATGAGATGGCCTTAAATGAATTGACCGGTAC30.20161290322580644No Hit
CTCGCAGGGATGAGATGGCCTTAAAGGAATTGACCGGTAC30.20161290322580644No Hit
CTCGCAGGGATGAGATGGACTCCAAGGAATTGACGGGTAC30.20161290322580644No Hit
CTCGCAGGGATGAGGTGGACTCAAATGAATTGACGGGTAC20.13440860215053765No Hit
CTCGCAGGGCTCGTCTTAATCCGCAACATCTTTTGATACC20.13440860215053765No Hit
CTCGCAGGGATGAGATGGCCCCGGTGCCTCTTTTGTGACG20.13440860215053765No Hit
CTCGCAGGGAAGTCACCAGATCGACTTTTTTTTTTTTTTT20.13440860215053765No Hit
CTCGCAGGGGGGCTTAGAAGCAGAACAGTTCCCATGTCCA20.13440860215053765No Hit
CTCGCAGGGGATCCAGTGCCAGATACCATTAGATTCAAGA20.13440860215053765No Hit
CTCGCAGGGATGAGATGGACTCAAAGGCATTGACGGGTAC20.13440860215053765No Hit
CTCGCAGGGATGAGATGGGACTTAAAGGAATTGACGGGTA20.13440860215053765No Hit
CTCGCAGGGATGAGATGGACTTCAAGGAATTGACGGGTAC20.13440860215053765No Hit
CTCGCAGGGATGAGATGGACTTAGGTTTGTGCTGGGTCTC20.13440860215053765No Hit
CTCGCAGGGGGAGTGCAGTGCTCTCGCAGGGATGAGATGG20.13440860215053765No Hit
CTCGCAGGGAGTGCAGTGCTCTCGCAGGGCTGAGATGGAC20.13440860215053765No Hit
CTCGCAGGGATGAGATGGACTTAAAGGAATTGACCGGCAC20.13440860215053765No Hit
CTCGCAGGGATGAGATGGCCTTCAAGGAATTGACGGGTAC20.13440860215053765No Hit
CTCGCAGGGATGAGATGGCCTCAAATGAATTGACGGGTCC20.13440860215053765No Hit
CTCGCAGGGATGAGATGGACTCAAAGGAATTGACCGGTCC20.13440860215053765No Hit
CTCGCAGGGATGAGCTGGACTTAAAGGAATTGACGGTGAG20.13440860215053765No Hit
CTCGCAGGGAGTGCAGTGCTCTCGCAGGGATGAGATGGAC20.13440860215053765No Hit
CTCGCAGGGATGAGCTGGACTCAAAGGAATTGACGGGTAC20.13440860215053765No Hit
CTCGCAGGGATGAGATGGCCTCCAAGGAATTGACGGGTAC20.13440860215053765No Hit
CTCGCAGGGATGAGATGGACTTACAGGAATTGACCGGTAC20.13440860215053765No Hit
CTCGCAGGGCTGGTACGCCGATCGACTTTTTTTTTTTTTT20.13440860215053765No Hit
CTCGCAGGGATGAGATGGACTTAAAGGCCTTGACGGGTAC20.13440860215053765No Hit
CTCGCAGGGATGAGATGGCCTTAACGGAATTGACGGGTAC20.13440860215053765No Hit
CTCGCAGGGATGAGATGGACTTAAATGCATTGACGGGTAC20.13440860215053765No Hit
CTCGCAGGGATGAGATGGCCTTCAAGGAATTGACCGGTAC20.13440860215053765No Hit
CTCGCAGGGATGAGATGGCCTTCAATGAATTGACGGGTAC20.13440860215053765No Hit
CTCGCAGGGATGAGATGGAGAAAAAAAAAAAAAAAAAAAA20.13440860215053765No Hit
CTCGCAGGGATGAGATGGACTCAAAGGACTTGACGGGTAC20.13440860215053765No Hit
CTCGCAGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCG20.13440860215053765No Hit
CTCGCAGGGCTGAGATGGACTCAAATGAATTGACGGGTAC20.13440860215053765No Hit
CTCGCAGGGATGAGATGGCAAAAAAAAAAAAAAAAAAAAA20.13440860215053765No Hit
CTCGCAGGGATGAGATGGCCTTAAAGGAATTGCCCGGTAC20.13440860215053765No Hit
CTCGCAGGGATGAGATGGACTTAAATGAATTGACGGATAC20.13440860215053765No Hit
CTCGCAGGGATGAGATGGCCTCAAAGGAATTGACGGGTCC20.13440860215053765No Hit
CTCGCAGGGGCAGAGTCGCCTTTTTTTTTTTTTTTTTTTT20.13440860215053765No Hit
CTCGCAGGGATGAGATGGACTTAACGGAATTGACCGGTAC20.13440860215053765No Hit
CTCGCAGGGGGGGTTGGGGATTTAGCTCAGTGGTAGCGCG20.13440860215053765No Hit
CTCGCAGGGGGGTTAAAGCGAGGCACATTTAAAGATTTGA20.13440860215053765No Hit
CTCGCAGGGATCACGCAGAGTCGACTTTTTTTTTTTTTTT20.13440860215053765No Hit
CTCGCAGGGATGAGATGGACTTAAATGAATTGACCGGTCC20.13440860215053765No Hit
CTCGCAGGGATGAGATGGACTTAAAGGACTTGACGGGTAC20.13440860215053765No Hit
CTCGCAGGGATGAGATGGCCTCACAGGAATTGACGGGTAC20.13440860215053765No Hit
CTCGCAGGGATGAGATGGACTTAAATGAAGTGACGGGTAC20.13440860215053765No Hit
CTCGCAGGGATGAGATGGCACTTAAATGAATTGACGGGTA20.13440860215053765No Hit
CTCGCAGGGATGAGATGGCCTCAACGGAATTGACCGGTAC20.13440860215053765No Hit
CTCGCAGGGCTGAGATGGACTTAAATGAATTGACCGGTCC20.13440860215053765No Hit
CTCGCAGGGATGAGATGGACTTAACTGAATTGACGGGTCC20.13440860215053765No Hit
CTCGCAGGGATGAGATGGCCTTAAATGAATTGACGGAGTA20.13440860215053765No Hit
CTCGCAGGGATGAGATGGCCTTACATGAATTGCCGGGTAC20.13440860215053765No Hit
CTCGCAGGGATGAGATGGGACTTAAATGAATTGACGGGTA20.13440860215053765No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATGGCC353.9735096E-734.014
GATGGAC451.1241355E-934.014
GGATGAG900.034.08
GCCTTAA200.001971197834.018
GGACTTA251.1973548E-434.017
GCAGGGA1150.034.04
GACGGGT600.034.032
ATGAATT307.026172E-634.025
ATGGCCT353.9735096E-734.015
AGGGATG950.034.06
GATGAGA850.034.09
ATGGACT402.1596861E-834.015
CGGGTAC551.8189894E-1234.034
GGCCTTA200.001971197834.017
CAGGGAT1000.034.05
ACGGGTA600.034.033
TGGACTT307.026172E-634.016
AGATGGC402.1596861E-834.013
AGATGGA451.1241355E-934.013
GGGATGA950.034.07
Produced by FastQC (version 0.11.5)