##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1526169_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1488 Sequences flagged as poor quality 0 Sequence length 40 %GC 52 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 24.464381720430108 30.0 4.0 33.0 4.0 35.0 2 14.286962365591398 13.0 4.0 23.0 4.0 30.0 3 15.054435483870968 14.0 4.0 25.0 4.0 31.0 4 13.47244623655914 10.0 4.0 22.0 4.0 30.0 5 16.552419354838708 12.0 4.0 30.0 4.0 33.0 6 11.43481182795699 4.0 4.0 18.0 4.0 27.0 7 13.70228494623656 4.0 4.0 24.0 4.0 29.0 8 12.856854838709678 4.0 4.0 21.0 4.0 28.0 9 13.28763440860215 4.0 4.0 23.0 4.0 29.0 10 8.942204301075268 4.0 4.0 11.0 4.0 20.0 11 15.692876344086022 4.0 4.0 29.0 4.0 33.0 12 16.267473118279568 4.0 4.0 31.0 4.0 34.0 13 13.418682795698924 4.0 4.0 24.0 4.0 31.0 14 15.174059139784946 4.0 4.0 30.0 4.0 33.0 15 12.468413978494624 4.0 4.0 23.0 4.0 31.0 16 13.048387096774194 4.0 4.0 26.0 4.0 31.0 17 13.076612903225806 4.0 4.0 26.0 4.0 31.0 18 12.631720430107526 4.0 4.0 24.0 4.0 31.0 19 11.206989247311828 4.0 4.0 19.0 4.0 30.0 20 13.299731182795698 4.0 4.0 28.0 4.0 33.0 21 10.755376344086022 4.0 4.0 19.0 4.0 31.0 22 10.558467741935484 4.0 4.0 16.0 4.0 31.0 23 8.971102150537634 4.0 4.0 4.0 4.0 28.0 24 8.830645161290322 4.0 4.0 4.0 4.0 28.0 25 8.678763440860216 4.0 4.0 4.0 4.0 28.0 26 8.301075268817204 4.0 4.0 4.0 4.0 28.0 27 8.442876344086022 4.0 4.0 4.0 4.0 30.0 28 7.616263440860215 4.0 4.0 4.0 4.0 24.0 29 7.728494623655914 4.0 4.0 4.0 4.0 27.0 30 7.162634408602151 4.0 4.0 4.0 4.0 22.0 31 6.932123655913978 4.0 4.0 4.0 4.0 23.0 32 6.249327956989247 4.0 4.0 4.0 4.0 15.0 33 6.145161290322581 4.0 4.0 4.0 4.0 13.0 34 6.662634408602151 4.0 4.0 4.0 4.0 15.0 35 5.70497311827957 4.0 4.0 4.0 4.0 4.0 36 5.974462365591398 4.0 4.0 4.0 4.0 4.0 37 5.88239247311828 4.0 4.0 4.0 4.0 4.0 38 5.804435483870968 4.0 4.0 4.0 4.0 4.0 39 5.728494623655914 4.0 4.0 4.0 4.0 4.0 40 5.936155913978495 4.0 4.0 4.0 4.0 4.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1 1 7.143560606060603 1 2 5.868181818181819 1 3 -7.721212121212121 1 4 12.782007575757575 1 5 0.6568181818181849 1 6 12.12310606060606 1 7 9.78503787878788 1 8 7.1 1 9 13.94128787878788 1 10 6.498295454545454 1 11 9.660416666666666 1 12 11.955303030303028 1 13 -2.6564393939393938 1 14 11.378409090909091 1 15 4.313257575757575 1 16 12.035037878787877 1 17 14.365909090909089 1 18 10.6280303030303 1 19 1.094507575757575 1 20 13.536363636363637 1 21 23.580681818181816 1 22 20.060227272727275 1 23 15.094696969696969 1 24 17.298295454545453 1 25 20.972537878787875 1 26 15.751893939393941 1 27 21.170454545454547 1 28 21.09090909090909 1 29 22.539772727272727 1 30 20.90340909090909 1 31 25.80681818181818 1 32 26.00568181818182 1 33 27.102272727272727 1 34 30.261363636363637 1 35 13.960227272727273 1 36 -2.340909090909091 1 37 -2.340909090909091 1 38 -2.3522727272727275 1 39 -2.221590909090909 1 40 -2.6761363636363633 5 1 8.643560606060603 5 2 12.868181818181819 5 3 0.7787878787878793 5 4 0.28200757575757507 5 5 11.656818181818185 5 6 17.123106060606062 5 7 7.785037878787881 5 8 7.1 5 9 -6.558712121212119 5 10 10.498295454545454 5 11 -4.339583333333334 5 12 13.455303030303028 5 13 12.843560606060606 5 14 14.878409090909091 5 15 6.313257575757575 5 16 8.535037878787877 5 17 15.365909090909089 5 18 13.6280303030303 5 19 9.594507575757575 5 20 9.036363636363637 5 21 10.580681818181818 5 22 16.060227272727275 5 23 14.094696969696969 5 24 17.798295454545453 5 25 22.472537878787875 5 26 -0.7481060606060597 5 27 3.170454545454546 5 28 -0.40909090909090917 5 29 -1.4602272727272725 5 30 7.403409090909091 5 31 2.8068181818181817 5 32 10.005681818181818 5 33 10.102272727272727 5 34 8.761363636363637 5 35 7.4602272727272725 5 36 2.159090909090909 5 37 12.65909090909091 5 38 10.647727272727273 5 39 12.27840909090909 5 40 11.323863636363637 6 1 -8.856439393939397 6 2 -6.631818181818181 6 3 -6.721212121212121 6 4 -8.717992424242425 6 5 -4.343181818181815 6 6 -6.8768939393939394 6 7 -2.2149621212121193 6 8 -0.9000000000000004 6 9 -3.0587121212121193 6 10 -4.501704545454546 6 11 -0.33958333333333357 6 12 -2.0446969696969717 6 13 -2.1564393939393938 6 14 -0.12159090909090864 6 15 -4.186742424242425 6 16 -1.4649621212121229 6 17 2.8659090909090885 6 18 2.6280303030303003 6 19 4.594507575757575 6 20 2.536363636363637 6 21 8.080681818181818 6 22 7.560227272727273 6 23 9.594696969696969 6 24 9.798295454545455 6 25 -4.027462121212123 6 26 -3.7481060606060597 6 27 -3.829545454545454 6 28 -3.409090909090909 6 29 -2.9602272727272725 6 30 -2.596590909090909 6 31 -3.1931818181818183 6 32 -2.4943181818181817 6 33 -2.3977272727272725 6 34 -2.7386363636363633 6 35 -2.0397727272727275 6 36 -2.340909090909091 6 37 -2.340909090909091 6 38 -2.3522727272727275 6 39 -2.221590909090909 6 40 -2.6761363636363633 7 1 -7.606439393939397 7 2 -8.881818181818181 7 3 -9.97121212121212 7 4 -9.467992424242425 7 5 -8.843181818181815 7 6 -6.3768939393939394 7 7 -7.714962121212119 7 8 -5.4 7 9 -7.558712121212119 7 10 -4.751704545454546 7 11 -6.589583333333334 7 12 -7.044696969696972 7 13 -6.156439393939394 7 14 -6.371590909090909 7 15 -5.186742424242425 7 16 -3.214962121212123 7 17 -3.3840909090909115 7 18 -3.6219696969696997 7 19 -3.405492424242425 7 20 -3.713636363636363 7 21 1.3306818181818176 7 22 1.310227272727273 7 23 -4.405303030303031 7 24 -4.201704545454545 7 25 -4.027462121212123 7 26 -3.7481060606060597 7 27 -3.829545454545454 7 28 -3.409090909090909 7 29 -2.9602272727272725 7 30 -2.596590909090909 7 31 -3.1931818181818183 7 32 -2.4943181818181817 7 33 -2.3977272727272725 7 34 -2.7386363636363633 7 35 -2.0397727272727275 7 36 -2.340909090909091 7 37 -2.340909090909091 7 38 -2.3522727272727275 7 39 -2.221590909090909 7 40 -2.6761363636363633 8 1 -10.68977272727273 8 2 -10.631818181818181 8 3 -11.72121212121212 8 4 -10.217992424242425 8 5 -13.343181818181815 8 6 -7.8768939393939394 8 7 -10.21496212121212 8 8 -8.9 8 9 -10.05871212121212 8 10 -5.501704545454546 8 11 -12.339583333333334 8 12 -14.044696969696972 8 13 -10.656439393939394 8 14 -12.121590909090909 8 15 -9.686742424242425 8 16 -9.964962121212123 8 17 -10.134090909090911 8 18 -9.3719696969697 8 19 -6.905492424242425 8 20 -10.463636363636363 8 21 -5.419318181818182 8 22 -5.939772727272727 8 23 -4.405303030303031 8 24 -4.201704545454545 8 25 -4.027462121212123 8 26 -3.7481060606060597 8 27 -3.829545454545454 8 28 -3.409090909090909 8 29 -2.9602272727272725 8 30 -2.596590909090909 8 31 -3.1931818181818183 8 32 -2.4943181818181817 8 33 -2.3977272727272725 8 34 -2.7386363636363633 8 35 -2.0397727272727275 8 36 -2.340909090909091 8 37 -2.340909090909091 8 38 -2.3522727272727275 8 39 -2.221590909090909 8 40 -2.6761363636363633 9 1 -7.856439393939397 9 2 -6.131818181818181 9 3 -7.721212121212121 9 4 -8.217992424242425 9 5 -2.843181818181815 9 6 -7.3768939393939394 9 7 -4.714962121212119 9 8 -0.9000000000000004 9 9 -3.0587121212121193 9 10 -1.5017045454545457 9 11 -6.339583333333334 9 12 -1.0446969696969717 9 13 -1.1564393939393938 9 14 -5.621590909090909 9 15 -3.686742424242425 9 16 4.035037878787877 9 17 3.8659090909090885 9 18 1.6280303030303003 9 19 3.094507575757575 9 20 -1.463636363636363 9 21 3.5806818181818176 9 22 8.060227272727273 9 23 9.094696969696969 9 24 2.798295454545455 9 25 9.472537878787877 9 26 7.75189393939394 9 27 7.670454545454546 9 28 8.09090909090909 9 29 8.539772727272727 9 30 8.90340909090909 9 31 2.3068181818181817 9 32 8.505681818181818 9 33 11.602272727272727 9 34 2.7613636363636367 9 35 11.960227272727273 9 36 13.15909090909091 9 37 12.65909090909091 9 38 13.147727272727273 9 39 13.27840909090909 9 40 12.823863636363637 10 1 8.843560606060606 10 2 7.9681818181818205 10 3 13.07878787878788 10 4 9.382007575757576 10 5 15.856818181818188 10 6 12.12310606060606 10 7 10.78503787878788 10 8 10.299999999999999 10 9 10.94128787878788 10 10 3.8982954545454547 10 11 9.460416666666667 10 12 11.55530303030303 10 13 6.943560606060608 10 14 12.07840909090909 10 15 9.913257575757576 10 16 6.035037878787877 10 17 8.66590909090909 10 18 8.028030303030299 10 19 4.694507575757575 10 20 8.336363636363638 10 21 3.980681818181818 10 22 -0.7397727272727277 10 23 -4.405303030303031 10 24 -4.201704545454545 10 25 -4.027462121212123 10 26 -3.7481060606060597 10 27 -3.829545454545454 10 28 -3.409090909090909 10 29 -2.9602272727272725 10 30 -2.596590909090909 10 31 -3.1931818181818183 10 32 -2.4943181818181817 10 33 -2.3977272727272725 10 34 -2.7386363636363633 10 35 -2.0397727272727275 10 36 -2.340909090909091 10 37 -2.340909090909091 10 38 -2.3522727272727275 10 39 -2.221590909090909 10 40 -2.6761363636363633 11 1 -7.856439393939397 11 2 -7.631818181818181 11 3 -8.22121212121212 11 4 -8.717992424242425 11 5 -4.343181818181815 11 6 -3.8768939393939394 11 7 -5.714962121212119 11 8 -3.4000000000000004 11 9 -6.058712121212119 11 10 -2.0017045454545457 11 11 1.1604166666666664 11 12 1.9553030303030283 11 13 3.3435606060606062 11 14 1.8784090909090914 11 15 4.313257575757575 11 16 2.535037878787877 11 17 2.3659090909090885 11 18 -0.3719696969696997 11 19 -0.9054924242424249 11 20 3.036363636363637 11 21 -5.419318181818182 11 22 -5.939772727272727 11 23 -4.405303030303031 11 24 -4.201704545454545 11 25 -4.027462121212123 11 26 -3.7481060606060597 11 27 -3.829545454545454 11 28 -3.409090909090909 11 29 -2.9602272727272725 11 30 -2.596590909090909 11 31 -3.1931818181818183 11 32 -2.4943181818181817 11 33 -2.3977272727272725 11 34 -2.7386363636363633 11 35 -2.0397727272727275 11 36 -2.340909090909091 11 37 -2.340909090909091 11 38 -2.3522727272727275 11 39 -2.221590909090909 11 40 -2.6761363636363633 12 1 -2.0231060606060645 12 2 -10.631818181818181 12 3 -11.72121212121212 12 4 -10.217992424242425 12 5 -13.343181818181815 12 6 -7.8768939393939394 12 7 -10.21496212121212 12 8 -8.9 12 9 -10.05871212121212 12 10 -5.501704545454546 12 11 -12.339583333333334 12 12 -14.044696969696972 12 13 -10.656439393939394 12 14 -12.121590909090909 12 15 -9.686742424242425 12 16 -9.964962121212123 12 17 -10.134090909090911 12 18 -9.3719696969697 12 19 -6.905492424242425 12 20 -10.463636363636363 12 21 -5.419318181818182 12 22 -5.939772727272727 12 23 -4.405303030303031 12 24 -4.201704545454545 12 25 -4.027462121212123 12 26 -3.7481060606060597 12 27 -3.829545454545454 12 28 -3.409090909090909 12 29 -2.9602272727272725 12 30 -2.596590909090909 12 31 -3.1931818181818183 12 32 -2.4943181818181817 12 33 -2.3977272727272725 12 34 -2.7386363636363633 12 35 -2.0397727272727275 12 36 -2.340909090909091 12 37 -2.340909090909091 12 38 -2.3522727272727275 12 39 -2.221590909090909 12 40 -2.6761363636363633 13 1 4.143560606060603 13 2 17.368181818181817 13 3 13.77878787878788 13 4 15.782007575757575 13 5 -1.3431818181818151 13 6 19.123106060606062 13 7 7.785037878787881 13 8 11.1 13 9 11.44128787878788 13 10 19.498295454545456 13 11 15.660416666666666 13 12 1.9553030303030283 13 13 11.343560606060606 13 14 6.878409090909091 13 15 8.313257575757575 13 16 18.035037878787875 13 17 8.865909090909089 13 18 15.1280303030303 13 19 10.594507575757575 13 20 7.536363636363637 13 21 14.580681818181818 13 22 18.060227272727275 13 23 21.09469696969697 13 24 21.798295454545453 13 25 24.472537878787875 13 26 19.25189393939394 13 27 17.670454545454547 13 28 8.09090909090909 13 29 18.539772727272727 13 30 16.40340909090909 13 31 22.80681818181818 13 32 10.505681818181818 13 33 -2.3977272727272725 13 34 -2.7386363636363633 13 35 -2.0397727272727275 13 36 -2.340909090909091 13 37 -2.340909090909091 13 38 -2.3522727272727275 13 39 -2.221590909090909 13 40 -2.6761363636363633 14 1 8.643560606060603 14 2 10.368181818181819 14 3 17.27878787878788 14 4 13.782007575757575 14 5 10.656818181818185 14 6 -3.8768939393939394 14 7 10.78503787878788 14 8 13.1 14 9 15.94128787878788 14 10 19.498295454545456 14 11 6.660416666666666 14 12 10.955303030303028 14 13 21.343560606060606 14 14 19.87840909090909 14 15 15.313257575757575 14 16 19.035037878787875 14 17 20.86590909090909 14 18 19.6280303030303 14 19 22.094507575757575 14 20 8.536363636363637 14 21 17.580681818181816 14 22 16.060227272727275 14 23 17.59469696969697 14 24 1.798295454545455 14 25 25.972537878787875 14 26 11.251893939393941 14 27 9.170454545454547 14 28 12.59090909090909 14 29 10.039772727272727 14 30 2.403409090909091 14 31 12.806818181818182 14 32 -1.4943181818181817 14 33 17.602272727272727 14 34 5.261363636363637 14 35 1.9602272727272725 14 36 23.65909090909091 14 37 21.65909090909091 14 38 22.647727272727273 14 39 23.77840909090909 14 40 19.323863636363637 15 1 -7.356439393939397 15 2 -10.631818181818181 15 3 -11.72121212121212 15 4 -10.217992424242425 15 5 -13.343181818181815 15 6 -7.8768939393939394 15 7 -10.21496212121212 15 8 -8.9 15 9 -10.05871212121212 15 10 -5.501704545454546 15 11 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-3.0017045454545457 16 11 -1.3395833333333336 16 12 -0.04469696969697168 16 13 -3.1564393939393938 16 14 1.3784090909090914 16 15 -2.186742424242425 16 16 0.5350378787878771 16 17 0.3659090909090885 16 18 3.1280303030303003 16 19 -2.905492424242425 16 20 1.036363636363637 16 21 6.580681818181818 16 22 6.060227272727273 16 23 5.594696969696969 16 24 7.798295454545455 16 25 7.972537878787877 16 26 8.251893939393941 16 27 9.670454545454547 16 28 -3.409090909090909 16 29 -2.9602272727272725 16 30 -2.596590909090909 16 31 -3.1931818181818183 16 32 -2.4943181818181817 16 33 -2.3977272727272725 16 34 -2.7386363636363633 16 35 -2.0397727272727275 16 36 -2.340909090909091 16 37 -2.340909090909091 16 38 -2.3522727272727275 16 39 -2.221590909090909 16 40 -2.6761363636363633 17 1 6.893560606060603 17 2 16.618181818181817 17 3 14.52878787878788 17 4 9.782007575757575 17 5 4.156818181818185 17 6 11.12310606060606 17 7 10.03503787878788 17 8 13.6 17 9 14.44128787878788 17 10 9.498295454545454 17 11 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117 15 -9.686742424242425 117 16 -9.964962121212123 117 17 -10.134090909090911 117 18 -9.3719696969697 117 19 -6.905492424242425 117 20 -10.463636363636363 117 21 -5.419318181818182 117 22 -5.939772727272727 117 23 -4.405303030303031 117 24 -4.201704545454545 117 25 -4.027462121212123 117 26 -3.7481060606060597 117 27 -3.829545454545454 117 28 -3.409090909090909 117 29 -2.9602272727272725 117 30 -2.596590909090909 117 31 -3.1931818181818183 117 32 -2.4943181818181817 117 33 -2.3977272727272725 117 34 -2.7386363636363633 117 35 -2.0397727272727275 117 36 -2.340909090909091 117 37 -2.340909090909091 117 38 -2.3522727272727275 117 39 -2.221590909090909 117 40 -2.6761363636363633 >>END_MODULE >>Per sequence quality scores fail #Quality Count 4 641.0 5 14.0 6 81.0 7 17.0 8 11.0 9 27.0 10 48.0 11 42.0 12 54.0 13 75.0 14 56.0 15 58.0 16 51.0 17 39.0 18 37.0 19 41.0 20 30.0 21 23.0 22 23.0 23 28.0 24 23.0 25 18.0 26 27.0 27 4.0 28 12.0 29 3.0 30 3.0 31 1.0 32 0.0 33 0.0 34 1.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 0.0 0.0 0.0 100.0 2 0.0 0.0 100.0 0.0 3 0.0 0.0 0.0 100.0 4 100.0 0.0 0.0 0.0 5 0.0 0.0 0.0 100.0 6 0.0 100.0 0.0 0.0 7 99.39434724091521 0.4037685060565276 0.0 0.2018842530282638 8 98.453261600538 0.3362474781439139 0.3362474781439139 0.8742434431741762 9 98.25268817204301 1.0080645161290323 0.47043010752688175 0.2688172043010753 10 13.98789509078682 74.1761936785474 3.362474781439139 8.47343644922663 11 9.61021505376344 10.887096774193548 73.38709677419355 6.115591397849462 12 78.36021505376344 6.182795698924731 8.064516129032258 7.39247311827957 13 13.5752688172043 69.08602150537635 8.400537634408602 8.938172043010754 14 71.10215053763442 9.946236559139784 10.28225806451613 8.669354838709678 15 7.93010752688172 68.54838709677419 12.096774193548388 11.424731182795698 16 11.895161290322582 7.795698924731183 72.37903225806451 7.93010752688172 17 73.25268817204301 8.803763440860216 8.198924731182796 9.744623655913978 18 72.31182795698925 9.274193548387096 8.60215053763441 9.811827956989246 19 10.080645161290322 46.034946236559136 6.78763440860215 37.096774193548384 20 9.005376344086022 11.35752688172043 10.483870967741936 69.15322580645162 21 7.258064516129033 8.60215053763441 69.28763440860214 14.85215053763441 22 8.736559139784946 10.551075268817204 48.723118279569896 31.989247311827956 23 10.349462365591398 64.24731182795699 9.946236559139784 15.456989247311828 24 9.274193548387096 64.18010752688173 8.803763440860216 17.741935483870968 25 11.028917283120377 65.77000672494955 8.406186953597848 14.794889038332212 26 38.35800807537012 13.660834454912516 35.32974427994616 12.6514131897712 27 65.45698924731182 11.223118279569892 12.365591397849462 10.954301075268818 28 9.744623655913978 64.11290322580645 11.559139784946236 14.583333333333334 29 8.338937457969067 62.74377942165433 14.122394082044384 14.794889038332212 30 8.131720430107526 9.946236559139784 70.49731182795699 11.424731182795698 31 9.145931405514458 9.41492938802959 72.6294552790854 8.809683927370545 32 61.22311827956989 10.483870967741936 17.405913978494624 10.887096774193548 33 10.080645161290322 59.07258064516129 14.583333333333334 16.263440860215052 34 7.594086021505377 10.013440860215054 17.338709677419356 65.05376344086021 35 49.73118279569893 9.744623655913978 16.33064516129032 24.193548387096776 36 58.66935483870967 9.67741935483871 18.615591397849464 13.03763440860215 37 51.81451612903226 10.013440860215054 24.663978494623656 13.508064516129032 38 8.53494623655914 11.693548387096774 65.92741935483872 13.844086021505376 39 5.913978494623656 52.083333333333336 19.153225806451612 22.849462365591396 40 9.543010752688172 14.24731182795699 18.010752688172044 58.1989247311828 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.5 17 1.0 18 1.0 19 0.5 20 0.0 21 1.5 22 3.0 23 3.0 24 3.0 25 3.0 26 4.5 27 6.0 28 6.0 29 6.5 30 7.0 31 10.0 32 13.0 33 13.0 34 12.0 35 11.0 36 13.0 37 15.0 38 15.0 39 23.0 40 31.0 41 33.5 42 36.0 43 36.0 44 47.5 45 59.0 46 112.0 47 165.0 48 165.0 49 227.0 50 289.0 51 301.0 52 313.0 53 313.0 54 248.5 55 184.0 56 140.5 57 97.0 58 97.0 59 86.0 60 75.0 61 62.0 62 49.0 63 49.0 64 46.5 65 44.0 66 36.0 67 28.0 68 28.0 69 24.5 70 21.0 71 17.0 72 13.0 73 13.0 74 11.5 75 10.0 76 8.5 77 7.0 78 7.0 79 6.5 80 6.0 81 4.0 82 2.0 83 2.0 84 1.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.13440860215053765 8 0.06720430107526883 9 0.0 10 0.06720430107526883 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.06720430107526883 26 0.13440860215053765 27 0.0 28 0.0 29 0.06720430107526883 30 0.0 31 0.06720430107526883 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 37 0.0 38 0.0 39 0.0 40 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 40 1488.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 74.46236559139786 #Duplication Level Percentage of deduplicated Percentage of total 1 91.87725631768953 68.41397849462365 2 4.6028880866426 6.854838709677419 3 1.444043321299639 3.225806451612903 4 0.5415162454873645 1.6129032258064515 5 0.2707581227436823 1.0080645161290323 6 0.0 0.0 7 0.18050541516245489 0.9408602150537635 8 0.18050541516245489 1.0752688172043012 9 0.0 0.0 >10 0.8122743682310468 13.306451612903224 >50 0.09025270758122744 3.5618279569892475 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source CTCGCAGGGATGAGATGGACTTAAAGGAATTGACGGGTAC 53 3.5618279569892475 No Hit CTCGCAGGGATGAGATGGACTTAAATGAATTGACGGGTAC 47 3.1586021505376345 No Hit CTCGCAGGGATGAGATGGCCTTAAAGGAATTGACGGGTAC 36 2.4193548387096775 No Hit CTCGCAGGGATGAGATGGCCTTAAATGAATTGACGGGTAC 30 2.0161290322580645 No Hit CTCGCAGGGATGAGATGGACTCAAAGGAATTGACGGGTAC 21 1.411290322580645 No Hit CTCGCAGGGATGAGATGGACTCAAATGAATTGACGGGTAC 19 1.2768817204301075 No Hit CTCGCAGGGATGAGATGGCCTCAAAGGAATTGACGGGTAC 13 0.8736559139784945 No Hit CTCGCAGGGATGAGATGGCCTCAAATGAATTGACGGGTAC 12 0.8064516129032258 No Hit CTCGCAGGGATGAGCTGGACTTAAAGGAATTGACGGGTAC 10 0.6720430107526881 No Hit CTCGCAGGGATGAGATGGACTTAAATGAATTGACCGGTAC 10 0.6720430107526881 No Hit CTCGCAGGGATGAGATGGACTCAAAGGAATTGACCGGTAC 8 0.5376344086021506 No Hit CTCGCAGGGATGAGATGGACTTAACGGAATTGACGGGTAC 8 0.5376344086021506 No Hit CTCGCAGGGATGAGATGGACTTAAAGGAATTGACCGGTAC 7 0.47043010752688175 No Hit CTCGCAGGGATGAGATGGACTTACATGAATTGACGGGTAC 7 0.47043010752688175 No Hit CTCGCAGGGATGAGATGGACTTAAAGGAATTGACGGGTCC 5 0.33602150537634407 No Hit CTCGCAGGGATGAGATGGCCTTACAGGAATTGACGGGTAC 5 0.33602150537634407 No Hit CTCGCAGGGATGAGATGGACTTACATGAATTGACCGGTAC 5 0.33602150537634407 No Hit CTCGCAGGGATGAGATGGACTTAAATGAATTGCCGGGTAC 4 0.2688172043010753 No Hit CTCGCAGGGATGAGCTGGACTTAAATGAATTGACGGGTAC 4 0.2688172043010753 No Hit CTCGCAGGGATGAGATGGACTCAACGGAATTGACGGGTAC 4 0.2688172043010753 No Hit CTCGCAGGGATGAGATGGACTTAACTGAATTGACGGGTAC 4 0.2688172043010753 No Hit CTCGCAGGGATGAGATGGACTCCAATGAATTGACCGGTAC 4 0.2688172043010753 No Hit CTCGCAGGGATGAGATGGACTCACAGGAATTGACGGGTAC 4 0.2688172043010753 No Hit CTCGCAGGGGCTGGTTGTGGGGGTGGGGGAGATGTTTTAT 3 0.20161290322580644 No Hit CTCGCAGGGATGAGATGGACTTAAAGGAATTGACGGATAC 3 0.20161290322580644 No Hit CTCGCAGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCG 3 0.20161290322580644 No Hit CTCGCAGGGATGAGATGGACTCCAATGAATTGACGGGTAC 3 0.20161290322580644 No Hit CTCGCAGGGGGGAATTCGTGGAGAAAGAAATGGCTCCCCC 3 0.20161290322580644 No Hit CTCGCAGGGATGAGATGGCCTTAAAGGAATTGACGGGTCC 3 0.20161290322580644 No Hit CTCGCAGGGATGAGATGGCCTTAAATGACTTGACGGGTAC 3 0.20161290322580644 No Hit CTCGCAGGGATGAGATGGCCTTCAATGAATTGACCGGTAC 3 0.20161290322580644 No Hit CTCGCAGGGATGAGATGGCCTTAAATGAATTGCCGGGTAC 3 0.20161290322580644 No Hit CTCGCAGGGATGAGATGGACTTAAATGAATTGACGGGTCC 3 0.20161290322580644 No Hit CTCGCAGGGATGAGATGGACTCAACTGAATTGACGGGTAC 3 0.20161290322580644 No Hit CTCGCAGGGATGAGATGGACTTACAGGAATTGACGGGTAC 3 0.20161290322580644 No Hit CTCGCAGGGATGAGATGGCCTTAAAGGACTTGACGGGTAC 3 0.20161290322580644 No Hit CTCGCAGGGATGAGATGGCCTTAAATGAATTGACCGGTAC 3 0.20161290322580644 No Hit CTCGCAGGGATGAGATGGCCTTAAAGGAATTGACCGGTAC 3 0.20161290322580644 No Hit CTCGCAGGGATGAGATGGACTCCAAGGAATTGACGGGTAC 3 0.20161290322580644 No Hit CTCGCAGGGATGAGGTGGACTCAAATGAATTGACGGGTAC 2 0.13440860215053765 No Hit CTCGCAGGGCTCGTCTTAATCCGCAACATCTTTTGATACC 2 0.13440860215053765 No Hit CTCGCAGGGATGAGATGGCCCCGGTGCCTCTTTTGTGACG 2 0.13440860215053765 No Hit CTCGCAGGGAAGTCACCAGATCGACTTTTTTTTTTTTTTT 2 0.13440860215053765 No Hit CTCGCAGGGGGGCTTAGAAGCAGAACAGTTCCCATGTCCA 2 0.13440860215053765 No Hit CTCGCAGGGGATCCAGTGCCAGATACCATTAGATTCAAGA 2 0.13440860215053765 No Hit CTCGCAGGGATGAGATGGACTCAAAGGCATTGACGGGTAC 2 0.13440860215053765 No Hit CTCGCAGGGATGAGATGGGACTTAAAGGAATTGACGGGTA 2 0.13440860215053765 No Hit CTCGCAGGGATGAGATGGACTTCAAGGAATTGACGGGTAC 2 0.13440860215053765 No Hit CTCGCAGGGATGAGATGGACTTAGGTTTGTGCTGGGTCTC 2 0.13440860215053765 No Hit CTCGCAGGGGGAGTGCAGTGCTCTCGCAGGGATGAGATGG 2 0.13440860215053765 No Hit CTCGCAGGGAGTGCAGTGCTCTCGCAGGGCTGAGATGGAC 2 0.13440860215053765 No Hit CTCGCAGGGATGAGATGGACTTAAAGGAATTGACCGGCAC 2 0.13440860215053765 No Hit CTCGCAGGGATGAGATGGCCTTCAAGGAATTGACGGGTAC 2 0.13440860215053765 No Hit CTCGCAGGGATGAGATGGCCTCAAATGAATTGACGGGTCC 2 0.13440860215053765 No Hit CTCGCAGGGATGAGATGGACTCAAAGGAATTGACCGGTCC 2 0.13440860215053765 No Hit CTCGCAGGGATGAGCTGGACTTAAAGGAATTGACGGTGAG 2 0.13440860215053765 No Hit CTCGCAGGGAGTGCAGTGCTCTCGCAGGGATGAGATGGAC 2 0.13440860215053765 No Hit CTCGCAGGGATGAGCTGGACTCAAAGGAATTGACGGGTAC 2 0.13440860215053765 No Hit CTCGCAGGGATGAGATGGCCTCCAAGGAATTGACGGGTAC 2 0.13440860215053765 No Hit CTCGCAGGGATGAGATGGACTTACAGGAATTGACCGGTAC 2 0.13440860215053765 No Hit CTCGCAGGGCTGGTACGCCGATCGACTTTTTTTTTTTTTT 2 0.13440860215053765 No Hit CTCGCAGGGATGAGATGGACTTAAAGGCCTTGACGGGTAC 2 0.13440860215053765 No Hit CTCGCAGGGATGAGATGGCCTTAACGGAATTGACGGGTAC 2 0.13440860215053765 No Hit CTCGCAGGGATGAGATGGACTTAAATGCATTGACGGGTAC 2 0.13440860215053765 No Hit CTCGCAGGGATGAGATGGCCTTCAAGGAATTGACCGGTAC 2 0.13440860215053765 No Hit CTCGCAGGGATGAGATGGCCTTCAATGAATTGACGGGTAC 2 0.13440860215053765 No Hit CTCGCAGGGATGAGATGGAGAAAAAAAAAAAAAAAAAAAA 2 0.13440860215053765 No Hit CTCGCAGGGATGAGATGGACTCAAAGGACTTGACGGGTAC 2 0.13440860215053765 No Hit CTCGCAGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCG 2 0.13440860215053765 No Hit CTCGCAGGGCTGAGATGGACTCAAATGAATTGACGGGTAC 2 0.13440860215053765 No Hit CTCGCAGGGATGAGATGGCAAAAAAAAAAAAAAAAAAAAA 2 0.13440860215053765 No Hit CTCGCAGGGATGAGATGGCCTTAAAGGAATTGCCCGGTAC 2 0.13440860215053765 No Hit CTCGCAGGGATGAGATGGACTTAAATGAATTGACGGATAC 2 0.13440860215053765 No Hit CTCGCAGGGATGAGATGGCCTCAAAGGAATTGACGGGTCC 2 0.13440860215053765 No Hit CTCGCAGGGGCAGAGTCGCCTTTTTTTTTTTTTTTTTTTT 2 0.13440860215053765 No Hit CTCGCAGGGATGAGATGGACTTAACGGAATTGACCGGTAC 2 0.13440860215053765 No Hit CTCGCAGGGGGGGTTGGGGATTTAGCTCAGTGGTAGCGCG 2 0.13440860215053765 No Hit CTCGCAGGGGGGTTAAAGCGAGGCACATTTAAAGATTTGA 2 0.13440860215053765 No Hit CTCGCAGGGATCACGCAGAGTCGACTTTTTTTTTTTTTTT 2 0.13440860215053765 No Hit CTCGCAGGGATGAGATGGACTTAAATGAATTGACCGGTCC 2 0.13440860215053765 No Hit CTCGCAGGGATGAGATGGACTTAAAGGACTTGACGGGTAC 2 0.13440860215053765 No Hit CTCGCAGGGATGAGATGGCCTCACAGGAATTGACGGGTAC 2 0.13440860215053765 No Hit CTCGCAGGGATGAGATGGACTTAAATGAAGTGACGGGTAC 2 0.13440860215053765 No Hit CTCGCAGGGATGAGATGGCACTTAAATGAATTGACGGGTA 2 0.13440860215053765 No Hit CTCGCAGGGATGAGATGGCCTCAACGGAATTGACCGGTAC 2 0.13440860215053765 No Hit CTCGCAGGGCTGAGATGGACTTAAATGAATTGACCGGTCC 2 0.13440860215053765 No Hit CTCGCAGGGATGAGATGGACTTAACTGAATTGACGGGTCC 2 0.13440860215053765 No Hit CTCGCAGGGATGAGATGGCCTTAAATGAATTGACGGAGTA 2 0.13440860215053765 No Hit CTCGCAGGGATGAGATGGCCTTACATGAATTGCCGGGTAC 2 0.13440860215053765 No Hit CTCGCAGGGATGAGATGGGACTTAAATGAATTGACGGGTA 2 0.13440860215053765 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0 0.0 0.0 0.0 0.0 11 0.0 0.0 0.0 0.0 0.0 12 0.0 0.0 0.0 0.0 0.0 13 0.0 0.0 0.0 0.0 0.0 14 0.0 0.0 0.0 0.0 0.0 15 0.0 0.0 0.0 0.0 0.0 16 0.0 0.0 0.0 0.0 0.0 17 0.0 0.0 0.0 0.0 0.0 18 0.0 0.0 0.0 0.0 0.0 19 0.0 0.0 0.0 0.0 0.0 20 0.0 0.0 0.0 0.0 0.0 21 0.0 0.0 0.0 0.0 0.0 22 0.0 0.0 0.0 0.0 0.0 23 0.0 0.0 0.0 0.0 0.0 24 0.0 0.0 0.0 0.0 0.0 25 0.0 0.0 0.0 0.0 0.0 26 0.0 0.0 0.0 0.0 0.0 27 0.0 0.0 0.0 0.0 0.0 28 0.0 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GATGGCC 35 3.9735096E-7 34.0 14 GATGGAC 45 1.1241355E-9 34.0 14 GGATGAG 90 0.0 34.0 8 GCCTTAA 20 0.0019711978 34.0 18 GGACTTA 25 1.1973548E-4 34.0 17 GCAGGGA 115 0.0 34.0 4 GACGGGT 60 0.0 34.0 32 ATGAATT 30 7.026172E-6 34.0 25 ATGGCCT 35 3.9735096E-7 34.0 15 AGGGATG 95 0.0 34.0 6 GATGAGA 85 0.0 34.0 9 ATGGACT 40 2.1596861E-8 34.0 15 CGGGTAC 55 1.8189894E-12 34.0 34 GGCCTTA 20 0.0019711978 34.0 17 CAGGGAT 100 0.0 34.0 5 ACGGGTA 60 0.0 34.0 33 TGGACTT 30 7.026172E-6 34.0 16 AGATGGC 40 2.1596861E-8 34.0 13 AGATGGA 45 1.1241355E-9 34.0 13 GGGATGA 95 0.0 34.0 7 >>END_MODULE