FastQCFastQC Report
Fri 17 Jun 2016
SRR1526167_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526167_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1303
Sequences flagged as poor quality0
Sequence length40
%GC52

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCGCAGGGTCACAGATCCTCTTCTGAGATGAGTTTTTGT171.3046815042210285No Hit
CTCGCAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGC110.844205679201842No Hit
CTCGCAGGGTCACAGATCCTCTTCTGAAGGAGATTGGGCG100.7674597083653107No Hit
CTCGCAGGGTACCTGGTTGATCCTGCCAGTAGCATATGCT90.6907137375287797No Hit
CTCGCAGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGA70.5372217958557176No Hit
CTCGCAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAG60.4604758250191865No Hit
CTCGCAGGGATGAATTGAGGGTGCAGCTTGCTGCTGCACA40.3069838833461243No Hit
CTCGCAGGGGTGTTCTTTATTAATATCCTAACACTCCTCG30.23023791250959325No Hit
CTCGCAGGGAGCATATGCTTGTCTCAAAGATTAAGCCATG30.23023791250959325No Hit
CTCGCAGGGTCCCAGATCCTCTTCTGAGATGAGTTTTTGT30.23023791250959325No Hit
CTCGCAGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCAC30.23023791250959325No Hit
CTCGCAGGGAACTGTGGTAATTCTAGAGCTAATACATGCC30.23023791250959325No Hit
CTCGCAGGGTCAAAGATCCTCTTCTGAGATGAGTTTTTGT30.23023791250959325No Hit
CTCGCAGGGGAGGAGAAGCCCGGTTTACGCTGATGACCGA30.23023791250959325No Hit
CTCGCAGGGAAGCAGTGGTATCAACGCAGAGTGCAGTTTT20.15349194167306215No Hit
CTCGCAGGGGTATTAACTTAGCTTATGTATCTATGTATGT20.15349194167306215No Hit
CTCGCAGGGCTCCTACTTGGATAACTGTGGTAATTCTAGA20.15349194167306215No Hit
CTCGCAGGGGCGGCACCGGCGAGTCAGAGGCCCTGAGTAT20.15349194167306215No Hit
CTCGCAGGGGGGGCTAGCAATAATTTTGTTTAACTTTAAG20.15349194167306215No Hit
CTCGCAGGGATCTTGGAAAATTTTGCCAAAGACCATTGCC20.15349194167306215No Hit
CTCGCAGGGTACCTGGTTGATCCTGCCAGTAGCATATGCC20.15349194167306215No Hit
CTCGCAGGGGGGGGGAAAAAAAAAAAAAAAAAAAAAAAAA20.15349194167306215No Hit
CTCGCAGGGGGCCGGTACAGTGAAACTGCGAATGGCTCAT20.15349194167306215No Hit
CTCGCAGGGGGGCCTCTACCACTGAGCCACGCCTACTTCT20.15349194167306215No Hit
CTCGCAGGGCAGCATCTCTCTGGAGGTTATCCTTCGGGAT20.15349194167306215No Hit
CTCGCAGGGGAATTGTTTTATCCAACTCATCCCTTGACAT20.15349194167306215No Hit
CTCGCAGGGGGGGGCTGGAGAGATGGCTCAGTGGTTAAGA20.15349194167306215No Hit
CTCGCAGGGTCAAAGATCCTCTTCTGAAGGAGATTGGGCG20.15349194167306215No Hit
CTCGCAGGGGCTTTCGGCCGCCGCAGCCATCTTCCAGTAA20.15349194167306215No Hit
CTCGCAGGGTCCCAGATCATCTTCTGAGATGAGTTTTTGT20.15349194167306215No Hit
CTCGCAGGGGCCCACTTCGCCATCATATTCGTAGGAGTAA20.15349194167306215No Hit
CTCGCAGGGACTGTATGACTATACACATAATTTTCCTTTT20.15349194167306215No Hit
CTCGCAGGGGGCTTTGGTGACTCTAGATAATATCGTATGC20.15349194167306215No Hit
CTCGCAGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGG20.15349194167306215No Hit
CTCGCAGGGAGACAAATGACAGCAATTCATGGTTCAAGTA20.15349194167306215No Hit
CTCGCAGGGGGGGAGGTAGTGACGAAAAATAACAATACAG20.15349194167306215No Hit
CTCGCAGGGTCACAGATCCTCTTCTGAAGGACATTGGGCG20.15349194167306215No Hit
CTCGCAGGGACAGTAAGTCATTACTTTGATGTATTTTCAT20.15349194167306215No Hit
CTCGCAGGGATGAAAACTGCCGAGGAGAACTTGGACCGCA20.15349194167306215No Hit
CTCGCAGGGTAGATATGTTTTGTTTTCTCTTTTTTTTAAA20.15349194167306215No Hit
CTCGCAGGGAAAGACACAGTTGTACACTGAATTTTGTATA20.15349194167306215No Hit
CTCGCAGGGTGATTTAATATTACTACCCCTGACATTCACG20.15349194167306215No Hit
CTCGCAGGGGGGGCTGGAGAGATGGCTCAGCGGTTAAGAG20.15349194167306215No Hit
CTCGCAGGGAGGCTGGGTTTTGTCAGAGATTGATAAACTG20.15349194167306215No Hit
CTCGCAGGGTCACAGATCCTCTCCTGAAGGAGATTGGGCG20.15349194167306215No Hit
CTCGCAGGGGGAATCAGGGTTCGATTCCGGAGAGGGAGCC20.15349194167306215No Hit
CTCGCAGGGAAATCAGTTATGGTTCCTTTGGTCGCTCGCT20.15349194167306215No Hit
CTCGCAGGGCTCCAAGGAAGGCAGCAGGCGCGCAAATTAC20.15349194167306215No Hit
CTCGCAGGGGGCTGACTGTGCCACCGTACCTCCTGCTATC20.15349194167306215No Hit
CTCGCAGGGATTGGAGGGCAAGTCTGGTGCCAGCAGCCGC20.15349194167306215No Hit
CTCGCAGGGAGTTATGGTTCCTTTGGTCGCTCGCTCCTCT20.15349194167306215No Hit
CTCGCAGGGAACATTTCCTGGGCCTTTCAGGAATACCACG20.15349194167306215No Hit
CTCGCAGGGAATTTTGTGAGGCTGTGTTGGAAATAACCCG20.15349194167306215No Hit
CTCGCAGGGGTCTCAAAGATTAAGCCATGCATGTCTAAGT20.15349194167306215No Hit
CTCGCAGGGTGCTATGTATTTTTTTAAATGTTCAATATGA20.15349194167306215No Hit
CTCGCAGGGGCAAGAAAATTGACTAATGCTGCTCATTCCT20.15349194167306215No Hit
CTCGCAGGGTAAGTTCAATTTTAAACTTGCTAAAAAAACA20.15349194167306215No Hit
CTCGCAGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTT20.15349194167306215No Hit
CTCGCAGGGAGTGATAATGAGGTTTAAGGGGATTTAGGAC20.15349194167306215No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGGGTC200.001963918633.730776
GCAGGGT306.746417E-633.730774
GCAGGGG551.8189894E-1233.730774
GCAGGGC200.001963918633.730774
CAGGGGT200.001963918633.730775
CAGGGGG200.001963918633.730775
CGCAGGG1200.033.730773
TCGCAGG1250.033.730772
CTCGCAG1300.033.730771
Produced by FastQC (version 0.11.5)