##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1526167_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1303 Sequences flagged as poor quality 0 Sequence length 40 %GC 52 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 14.936300844205679 4.0 4.0 30.0 4.0 33.0 2 10.655410590943976 4.0 4.0 17.0 4.0 29.0 3 9.558710667689946 4.0 4.0 15.0 4.0 26.0 4 9.674597083653108 4.0 4.0 14.0 4.0 26.0 5 10.330007674597084 4.0 4.0 18.0 4.0 29.0 6 8.200306983883346 4.0 4.0 8.0 4.0 23.0 7 9.918649270913278 4.0 4.0 16.0 4.0 28.0 8 9.891788181120491 4.0 4.0 14.0 4.0 29.0 9 9.401381427475057 4.0 4.0 10.0 4.0 27.0 10 9.386799693016117 4.0 4.0 10.0 4.0 28.0 11 9.486569455103607 4.0 4.0 8.0 4.0 30.0 12 8.980813507290867 4.0 4.0 4.0 4.0 28.0 13 8.817344589409055 4.0 4.0 4.0 4.0 27.0 14 8.859554873369149 4.0 4.0 4.0 4.0 27.0 15 8.808135072908673 4.0 4.0 4.0 4.0 28.0 16 7.647735993860322 4.0 4.0 4.0 4.0 24.0 17 7.5203376822716805 4.0 4.0 4.0 4.0 22.0 18 7.418265541059094 4.0 4.0 4.0 4.0 21.0 19 7.2133537989255565 4.0 4.0 4.0 4.0 20.0 20 7.1435149654643135 4.0 4.0 4.0 4.0 20.0 21 6.701458173445894 4.0 4.0 4.0 4.0 17.0 22 6.538756715272449 4.0 4.0 4.0 4.0 16.0 23 6.5518035303146585 4.0 4.0 4.0 4.0 16.0 24 6.333077513430545 4.0 4.0 4.0 4.0 15.0 25 6.614735226400614 4.0 4.0 4.0 4.0 18.0 26 6.868764389869532 4.0 4.0 4.0 4.0 19.0 27 6.8749040675364546 4.0 4.0 4.0 4.0 19.0 28 7.1320030698388335 4.0 4.0 4.0 4.0 23.0 29 6.571757482732156 4.0 4.0 4.0 4.0 15.0 30 6.814274750575595 4.0 4.0 4.0 4.0 19.0 31 7.102072141212586 4.0 4.0 4.0 4.0 22.0 32 6.984650805832694 4.0 4.0 4.0 4.0 20.0 33 6.853415195702226 4.0 4.0 4.0 4.0 20.0 34 7.083653108211819 4.0 4.0 4.0 4.0 23.0 35 6.97544128933231 4.0 4.0 4.0 4.0 23.0 36 6.838833461243285 4.0 4.0 4.0 4.0 18.0 37 6.7214121258633925 4.0 4.0 4.0 4.0 17.0 38 6.534151957022257 4.0 4.0 4.0 4.0 16.0 39 6.5993860322333076 4.0 4.0 4.0 4.0 15.0 40 6.623944742900997 4.0 4.0 4.0 4.0 17.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 2 1 -7.909313725490197 2 2 -4.492156862745098 2 3 -3.247794117647059 2 4 -3.855882352941177 2 5 -4.579901960784314 2 6 -3.075980392156863 2 7 -4.527941176470588 2 8 -4.285294117647059 2 9 -4.304411764705883 2 10 -4.330392156862747 2 11 -4.463480392156862 2 12 -3.581617647058823 2 13 -3.337254901960784 2 14 -3.399264705882352 2 15 -3.3443627450980404 2 16 -2.5561274509803926 2 17 -2.6620098039215696 2 18 -2.2845588235294114 2 19 -1.979656862745097 2 20 -1.990931372549019 2 21 -1.7186274509803923 2 22 -1.7362745098039216 2 23 -2.1377450980392156 2 24 -1.3813725490196083 2 25 -1.2926470588235288 2 26 -1.9740196078431378 2 27 -1.9617647058823522 2 28 -2.045588235294118 2 29 -1.8892156862745102 2 30 -1.9779411764705879 2 31 -2.2789215686274504 2 32 -2.32843137254902 2 33 -1.8808823529411764 2 34 -2.3014705882352944 2 35 -2.1946078431372555 2 36 -2.2338235294117643 2 37 -1.8504901960784315 2 38 -1.8063725490196072 2 39 -1.7034313725490193 2 40 -2.1642156862745097 3 1 -7.909313725490197 3 2 -4.492156862745098 3 3 -3.247794117647059 3 4 -3.855882352941177 3 5 -4.579901960784314 3 6 -3.075980392156863 3 7 -4.527941176470588 3 8 -4.285294117647059 3 9 -4.304411764705883 3 10 -4.330392156862747 3 11 -4.463480392156862 3 12 -3.581617647058823 3 13 -3.337254901960784 3 14 -3.399264705882352 3 15 -3.3443627450980404 3 16 -2.5561274509803926 3 17 -2.6620098039215696 3 18 -2.2845588235294114 3 19 -1.979656862745097 3 20 -1.990931372549019 3 21 -1.7186274509803923 3 22 -1.7362745098039216 3 23 -2.1377450980392156 3 24 -1.3813725490196083 3 25 -1.2926470588235288 3 26 -1.9740196078431378 3 27 -1.9617647058823522 3 28 -2.045588235294118 3 29 -1.8892156862745102 3 30 -1.9779411764705879 3 31 -2.2789215686274504 3 32 -2.32843137254902 3 33 -1.8808823529411764 3 34 -2.3014705882352944 3 35 -2.1946078431372555 3 36 -2.2338235294117643 3 37 -1.8504901960784315 3 38 -1.8063725490196072 3 39 -1.7034313725490193 3 40 -2.1642156862745097 5 1 -7.909313725490197 5 2 -4.492156862745098 5 3 -3.247794117647059 5 4 -3.855882352941177 5 5 -4.579901960784314 5 6 -3.075980392156863 5 7 -4.527941176470588 5 8 -4.285294117647059 5 9 -4.304411764705883 5 10 -4.330392156862747 5 11 -4.463480392156862 5 12 -3.581617647058823 5 13 -3.337254901960784 5 14 -3.399264705882352 5 15 -3.3443627450980404 5 16 -2.5561274509803926 5 17 -2.6620098039215696 5 18 -2.2845588235294114 5 19 -1.979656862745097 5 20 -1.990931372549019 5 21 -1.7186274509803923 5 22 -1.7362745098039216 5 23 -2.1377450980392156 5 24 -1.3813725490196083 5 25 -1.2926470588235288 5 26 -1.9740196078431378 5 27 -1.9617647058823522 5 28 -2.045588235294118 5 29 -1.8892156862745102 5 30 -1.9779411764705879 5 31 -2.2789215686274504 5 32 -2.32843137254902 5 33 -1.8808823529411764 5 34 -2.3014705882352944 5 35 -2.1946078431372555 5 36 -2.2338235294117643 5 37 -1.8504901960784315 5 38 -1.8063725490196072 5 39 -1.7034313725490193 5 40 -2.1642156862745097 8 1 0.7573529411764692 8 2 -4.492156862745098 8 3 -3.247794117647059 8 4 -3.855882352941177 8 5 -4.579901960784314 8 6 -3.075980392156863 8 7 -4.527941176470588 8 8 -4.285294117647059 8 9 -4.304411764705883 8 10 -4.330392156862747 8 11 -4.463480392156862 8 12 -3.581617647058823 8 13 -3.337254901960784 8 14 -3.399264705882352 8 15 -3.3443627450980404 8 16 -2.5561274509803926 8 17 -2.6620098039215696 8 18 -2.2845588235294114 8 19 -1.979656862745097 8 20 -1.990931372549019 8 21 -1.7186274509803923 8 22 -1.7362745098039216 8 23 -2.1377450980392156 8 24 -1.3813725490196083 8 25 -1.2926470588235288 8 26 -1.9740196078431378 8 27 -1.9617647058823522 8 28 -2.045588235294118 8 29 -1.8892156862745102 8 30 -1.9779411764705879 8 31 -2.2789215686274504 8 32 -2.32843137254902 8 33 -1.8808823529411764 8 34 -2.3014705882352944 8 35 -2.1946078431372555 8 36 -2.2338235294117643 8 37 -1.8504901960784315 8 38 -1.8063725490196072 8 39 -1.7034313725490193 8 40 -2.1642156862745097 9 1 16.090686274509803 9 2 6.507843137254902 9 3 1.752205882352941 9 4 11.644117647058824 9 5 14.920098039215686 9 6 2.924019607843137 9 7 17.472058823529412 9 8 15.714705882352941 9 9 18.695588235294117 9 10 3.6696078431372534 9 11 11.536519607843138 9 12 9.418382352941176 9 13 13.162745098039217 9 14 3.600735294117648 9 15 13.65563725490196 9 16 13.943872549019607 9 17 6.83799019607843 9 18 9.215441176470588 9 19 19.020343137254905 9 20 17.50906862745098 9 21 15.281372549019608 9 22 18.76372549019608 9 23 22.362254901960785 9 24 12.61862745098039 9 25 12.70735294117647 9 26 15.025980392156862 9 27 25.038235294117648 9 28 23.95441176470588 9 29 24.61078431372549 9 30 21.522058823529413 9 31 24.72107843137255 9 32 22.67156862745098 9 33 21.11911764705882 9 34 26.698529411764707 9 35 26.805392156862744 9 36 27.266176470588235 9 37 29.649509803921568 9 38 25.193627450980394 9 39 23.79656862745098 9 40 29.33578431372549 16 1 21.090686274509803 16 2 11.507843137254902 16 3 1.0855392156862749 16 4 12.477450980392156 16 5 14.086764705882354 16 6 22.25735294117647 16 7 22.805392156862744 16 8 22.71470588235294 16 9 23.362254901960785 16 10 19.002941176470586 16 11 21.53651960784314 16 12 21.75171568627451 16 13 22.99607843137255 16 14 27.267401960784312 16 15 26.65563725490196 16 16 17.777205882352938 16 17 20.004656862745097 16 18 9.048774509803923 16 19 14.353676470588235 16 20 16.342401960784315 16 21 12.948039215686276 16 22 12.263725490196078 16 23 15.862254901960785 16 24 14.61862745098039 16 25 16.70735294117647 16 26 21.692647058823532 16 27 21.704901960784316 16 28 24.95441176470588 16 29 10.110784313725489 16 30 19.355392156862745 16 31 20.054411764705883 16 32 22.33823529411765 16 33 25.11911764705882 16 34 25.03186274509804 16 35 20.138725490196077 16 36 11.766176470588235 16 37 19.149509803921568 16 38 12.526960784313726 16 39 13.29656862745098 16 40 21.83578431372549 18 1 -1.575980392156863 18 2 -0.8254901960784311 18 3 3.085539215686275 18 4 2.810784313725489 18 5 0.4200980392156861 18 6 -2.075980392156863 18 7 0.4720588235294123 18 8 -2.9519607843137257 18 9 -2.6377450980392156 18 10 2.6696078431372534 18 11 -0.13014705882352828 18 12 0.7517156862745109 18 13 5.329411764705882 18 14 2.600735294117648 18 15 -0.011029411764707397 18 16 -0.2227941176470596 18 17 3.3379901960784304 18 18 -0.2845588235294114 18 19 0.68700980392157 18 20 2.009068627450981 18 21 4.281372549019608 18 22 6.9303921568627445 18 23 6.52892156862745 18 24 -0.04803921568627523 18 25 0.7073529411764712 18 26 6.692647058823528 18 27 1.3715686274509808 18 28 3.954411764705882 18 29 1.7774509803921568 18 30 1.688725490196079 18 31 3.0544117647058835 18 32 7.338235294117646 18 33 2.1191176470588236 18 34 4.365196078431372 18 35 4.8053921568627445 18 36 7.766176470588236 18 37 7.4828431372549025 18 38 5.193627450980393 18 39 6.629901960784315 18 40 6.502450980392156 19 1 12.090686274509803 19 2 8.307843137254903 19 3 12.352205882352942 19 4 13.344117647058823 19 5 10.020098039215688 19 6 3.924019607843137 19 7 13.87205882352941 19 8 14.11470588235294 19 9 16.895588235294117 19 10 13.469607843137254 19 11 8.13651960784314 19 12 6.218382352941178 19 13 10.262745098039218 19 14 11.000735294117646 19 15 16.65563725490196 19 16 8.843872549019608 19 17 5.93799019607843 19 18 3.3154411764705882 19 19 2.2203431372549023 19 20 6.809068627450982 19 21 5.481372549019607 19 22 4.663725490196079 19 23 3.0622549019607836 19 24 6.218627450980391 19 25 2.1073529411764715 19 26 9.425980392156863 19 27 6.438235294117648 19 28 7.554411764705882 19 29 9.91078431372549 19 30 6.022058823529412 19 31 7.52107843137255 19 32 5.67156862745098 19 33 -0.4808823529411761 19 34 0.6985294117647056 19 35 3.205392156862745 19 36 3.166176470588236 19 37 -1.8504901960784315 19 38 -1.8063725490196072 19 39 -1.7034313725490193 19 40 -2.1642156862745097 20 1 -2.409313725490197 20 2 1.007843137254902 20 3 -0.997794117647059 20 4 -2.355882352941177 20 5 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1.0906862745098032 36 2 0.007843137254901933 36 3 5.752205882352941 36 4 2.144117647058823 36 5 0.9200980392156861 36 6 -0.07598039215686292 36 7 1.4720588235294123 36 8 4.214705882352941 36 9 2.6955882352941174 36 10 -0.3303921568627466 36 11 5.536519607843138 36 12 2.418382352941177 36 13 -0.837254901960784 36 14 5.600735294117648 36 15 5.65563725490196 36 16 4.943872549019607 36 17 6.33799019607843 36 18 5.715441176470589 36 19 -0.479656862745097 36 20 2.509068627450981 36 21 0.7813725490196077 36 22 2.7637254901960784 36 23 6.862254901960784 36 24 7.618627450980392 36 25 3.207352941176471 36 26 9.525980392156862 36 27 0.5382352941176478 36 28 8.454411764705881 36 29 2.11078431372549 36 30 11.022058823529413 36 31 8.72107843137255 36 32 7.17156862745098 36 33 11.119117647058824 36 34 12.198529411764707 36 35 12.305392156862744 36 36 12.266176470588235 36 37 1.6495098039215685 36 38 12.193627450980394 36 39 12.79656862745098 36 40 10.83578431372549 37 1 -7.909313725490197 37 2 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-2.2789215686274504 111 32 -2.32843137254902 111 33 -1.8808823529411764 111 34 -2.3014705882352944 111 35 -2.1946078431372555 111 36 -2.2338235294117643 111 37 -1.8504901960784315 111 38 -1.8063725490196072 111 39 -1.7034313725490193 111 40 -2.1642156862745097 113 1 -7.909313725490197 113 2 -4.492156862745098 113 3 -3.247794117647059 113 4 -3.855882352941177 113 5 -4.579901960784314 113 6 -3.075980392156863 113 7 -4.527941176470588 113 8 -4.285294117647059 113 9 -4.304411764705883 113 10 -4.330392156862747 113 11 -4.463480392156862 113 12 -3.581617647058823 113 13 -3.337254901960784 113 14 -3.399264705882352 113 15 -3.3443627450980404 113 16 -2.5561274509803926 113 17 -2.6620098039215696 113 18 -2.2845588235294114 113 19 -1.979656862745097 113 20 -1.990931372549019 113 21 -1.7186274509803923 113 22 -1.7362745098039216 113 23 -2.1377450980392156 113 24 -1.3813725490196083 113 25 -1.2926470588235288 113 26 -1.9740196078431378 113 27 -1.9617647058823522 113 28 -2.045588235294118 113 29 -1.8892156862745102 113 30 -1.9779411764705879 113 31 -2.2789215686274504 113 32 -2.32843137254902 113 33 -1.8808823529411764 113 34 -2.3014705882352944 113 35 -2.1946078431372555 113 36 -2.2338235294117643 113 37 -1.8504901960784315 113 38 -1.8063725490196072 113 39 -1.7034313725490193 113 40 -2.1642156862745097 114 1 -7.909313725490197 114 2 -4.492156862745098 114 3 -3.247794117647059 114 4 -3.855882352941177 114 5 -4.579901960784314 114 6 -3.075980392156863 114 7 -4.527941176470588 114 8 -4.285294117647059 114 9 -4.304411764705883 114 10 -4.330392156862747 114 11 -4.463480392156862 114 12 -3.581617647058823 114 13 -3.337254901960784 114 14 -3.399264705882352 114 15 -3.3443627450980404 114 16 -2.5561274509803926 114 17 -2.6620098039215696 114 18 -2.2845588235294114 114 19 -1.979656862745097 114 20 -1.990931372549019 114 21 -1.7186274509803923 114 22 -1.7362745098039216 114 23 -2.1377450980392156 114 24 -1.3813725490196083 114 25 -1.2926470588235288 114 26 -1.9740196078431378 114 27 -1.9617647058823522 114 28 -2.045588235294118 114 29 -1.8892156862745102 114 30 -1.9779411764705879 114 31 -2.2789215686274504 114 32 -2.32843137254902 114 33 -1.8808823529411764 114 34 -2.3014705882352944 114 35 -2.1946078431372555 114 36 -2.2338235294117643 114 37 -1.8504901960784315 114 38 -1.8063725490196072 114 39 -1.7034313725490193 114 40 -2.1642156862745097 116 1 -7.909313725490197 116 2 -4.492156862745098 116 3 -3.247794117647059 116 4 -3.855882352941177 116 5 -4.579901960784314 116 6 -3.075980392156863 116 7 -4.527941176470588 116 8 -4.285294117647059 116 9 -4.304411764705883 116 10 -4.330392156862747 116 11 -4.463480392156862 116 12 -3.581617647058823 116 13 -3.337254901960784 116 14 -3.399264705882352 116 15 -3.3443627450980404 116 16 -2.5561274509803926 116 17 -2.6620098039215696 116 18 -2.2845588235294114 116 19 -1.979656862745097 116 20 -1.990931372549019 116 21 -1.7186274509803923 116 22 -1.7362745098039216 116 23 -2.1377450980392156 116 24 -1.3813725490196083 116 25 -1.2926470588235288 116 26 -1.9740196078431378 116 27 -1.9617647058823522 116 28 -2.045588235294118 116 29 -1.8892156862745102 116 30 -1.9779411764705879 116 31 -2.2789215686274504 116 32 -2.32843137254902 116 33 -1.8808823529411764 116 34 -2.3014705882352944 116 35 -2.1946078431372555 116 36 -2.2338235294117643 116 37 -1.8504901960784315 116 38 -1.8063725490196072 116 39 -1.7034313725490193 116 40 -2.1642156862745097 119 1 13.757352941176471 119 2 17.507843137254902 119 3 11.418872549019609 119 4 14.144117647058824 119 5 16.753431372549016 119 6 17.924019607843135 119 7 17.13872549019608 119 8 15.048039215686273 119 9 13.02892156862745 119 10 15.002941176470586 119 11 4.536519607843138 119 12 -3.581617647058823 119 13 -3.337254901960784 119 14 -3.399264705882352 119 15 -3.3443627450980404 119 16 -2.5561274509803926 119 17 -2.6620098039215696 119 18 -2.2845588235294114 119 19 -1.979656862745097 119 20 -1.990931372549019 119 21 -1.7186274509803923 119 22 -1.7362745098039216 119 23 -2.1377450980392156 119 24 -1.3813725490196083 119 25 -1.2926470588235288 119 26 -1.9740196078431378 119 27 -1.9617647058823522 119 28 -2.045588235294118 119 29 -1.8892156862745102 119 30 -1.9779411764705879 119 31 -2.2789215686274504 119 32 -2.32843137254902 119 33 -1.8808823529411764 119 34 -2.3014705882352944 119 35 -2.1946078431372555 119 36 -2.2338235294117643 119 37 -1.8504901960784315 119 38 -1.8063725490196072 119 39 -1.7034313725490193 119 40 -2.1642156862745097 >>END_MODULE >>Per sequence quality scores fail #Quality Count 4 858.0 5 25.0 6 13.0 7 29.0 8 17.0 9 20.0 10 33.0 11 38.0 12 28.0 13 21.0 14 19.0 15 10.0 16 12.0 17 11.0 18 9.0 19 10.0 20 27.0 21 27.0 22 20.0 23 17.0 24 15.0 25 17.0 26 11.0 27 6.0 28 5.0 29 1.0 30 3.0 31 1.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 0.0 0.0 0.0 100.0 2 0.0 0.0 100.0 0.0 3 0.0 0.0 0.0 100.0 4 100.0 0.0 0.0 0.0 5 0.0 0.0 0.0 100.0 6 0.0 100.0 0.0 0.0 7 99.46236559139786 0.30721966205837176 0.0 0.2304147465437788 8 97.23289777094543 1.3066871637202153 0.5380476556495004 0.9223674096848578 9 98.15809669992326 1.1511895625479662 0.5372217958557176 0.15349194167306215 10 53.60983102918587 23.886328725038403 11.751152073732719 10.75268817204301 11 36.91481197237145 24.02148887183423 20.951650038372986 18.112049117421336 12 32.8472755180353 22.56331542594014 21.181887950882576 23.407521105141978 13 23.118279569892472 20.814132104454686 25.57603686635945 30.491551459293394 14 21.565617805065234 31.158864159631623 29.16346891788181 18.112049117421336 15 30.161166538756717 26.86108979278588 26.630851880276285 16.34689178818112 16 30.161166538756717 29.77743668457406 24.481964696853414 15.579432079815811 17 26.247122026093628 23.254029163468918 34.22870299309287 16.270145817344588 18 24.86569455103607 24.86569455103607 29.547198772064466 20.72141212586339 19 25.709900230237913 27.014581734458943 25.172678434382195 22.10283960092095 20 26.247122026093628 24.481964696853414 32.46354566385265 16.80736761320031 21 24.09823484267076 26.016884113584037 26.47735993860322 23.407521105141978 22 21.181887950882576 22.64006139677667 36.37759017651573 19.80046047582502 23 20.874904067536455 28.31926323867997 29.77743668457406 21.028396009209516 24 22.10283960092095 25.940138142747504 28.779739063699157 23.177283192632387 25 22.48656945510361 30.391404451266308 26.47735993860322 20.64466615502686 26 27.73497688751926 30.662557781201848 24.268104776579353 17.334360554699536 27 18.846153846153847 32.53846153846154 24.307692307692307 24.307692307692307 28 23.059185242121444 29.82321291314374 27.671022290545732 19.446579554189086 29 28.373168851195064 29.606784888203546 21.81958365458751 20.20046260601388 30 25.40291634689179 25.40291634689179 29.3937068303914 19.80046047582502 31 28.54950115118956 29.00997697620875 24.405218726016884 18.035303146584805 32 28.406466512702078 24.55735180908391 25.86605080831409 21.17013086989992 33 27.62854950115119 26.630851880276285 24.635456638526477 21.105141980046046 34 19.723714504988486 26.32386799693016 33.998465080583266 19.95395241749808 35 18.956254796623178 29.3937068303914 34.68917881811205 16.96085955487337 36 22.119815668202765 29.80030721966206 28.878648233486942 19.201228878648234 37 23.56101304681504 27.78204144282425 29.316960859554875 19.33998465080583 38 22.17958557175748 29.77743668457406 28.472755180353033 19.570222563315426 39 24.635456638526477 30.46815042210284 20.491174213353798 24.405218726016884 40 21.105141980046046 27.551803530314658 30.314658480429777 21.028396009209516 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.5 12 1.0 13 1.0 14 0.5 15 0.0 16 1.0 17 2.0 18 2.0 19 1.5 20 1.0 21 3.0 22 5.0 23 5.0 24 6.0 25 7.0 26 8.0 27 9.0 28 9.0 29 8.0 30 7.0 31 15.0 32 23.0 33 23.0 34 25.0 35 27.0 36 33.5 37 40.0 38 40.0 39 41.0 40 42.0 41 52.0 42 62.0 43 62.0 44 73.0 45 84.0 46 103.0 47 122.0 48 122.0 49 116.5 50 111.0 51 102.0 52 93.0 53 93.0 54 113.5 55 134.0 56 138.0 57 142.0 58 142.0 59 124.5 60 107.0 61 100.5 62 94.0 63 94.0 64 94.5 65 95.0 66 74.0 67 53.0 68 53.0 69 39.0 70 25.0 71 16.5 72 8.0 73 8.0 74 6.5 75 5.0 76 3.5 77 2.0 78 2.0 79 1.5 80 1.0 81 0.5 82 0.0 83 0.0 84 0.5 85 1.0 86 0.5 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.07674597083653108 8 0.15349194167306215 9 0.0 10 0.07674597083653108 11 0.0 12 0.0 13 0.07674597083653108 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.38372985418265537 27 0.23023791250959325 28 0.15349194167306215 29 0.4604758250191865 30 0.0 31 0.0 32 0.3069838833461243 33 0.0 34 0.0 35 0.0 36 0.07674597083653108 37 0.0 38 0.0 39 0.0 40 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 40 1303.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 91.09746738296239 #Duplication Level Percentage of deduplicated Percentage of total 1 95.02948609941028 86.56945510360707 2 3.7910699241786014 6.907137375287798 3 0.5897219882055602 1.6116653875671527 4 0.08424599831508003 0.3069838833461243 5 0.0 0.0 6 0.08424599831508003 0.4604758250191865 7 0.08424599831508003 0.5372217958557176 8 0.0 0.0 9 0.08424599831508003 0.6907137375287797 >10 0.2527379949452401 2.916346891788181 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source CTCGCAGGGTCACAGATCCTCTTCTGAGATGAGTTTTTGT 17 1.3046815042210285 No Hit CTCGCAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGC 11 0.844205679201842 No Hit CTCGCAGGGTCACAGATCCTCTTCTGAAGGAGATTGGGCG 10 0.7674597083653107 No Hit CTCGCAGGGTACCTGGTTGATCCTGCCAGTAGCATATGCT 9 0.6907137375287797 No Hit CTCGCAGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGA 7 0.5372217958557176 No Hit CTCGCAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAG 6 0.4604758250191865 No Hit CTCGCAGGGATGAATTGAGGGTGCAGCTTGCTGCTGCACA 4 0.3069838833461243 No Hit CTCGCAGGGGTGTTCTTTATTAATATCCTAACACTCCTCG 3 0.23023791250959325 No Hit CTCGCAGGGAGCATATGCTTGTCTCAAAGATTAAGCCATG 3 0.23023791250959325 No Hit CTCGCAGGGTCCCAGATCCTCTTCTGAGATGAGTTTTTGT 3 0.23023791250959325 No Hit CTCGCAGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCAC 3 0.23023791250959325 No Hit CTCGCAGGGAACTGTGGTAATTCTAGAGCTAATACATGCC 3 0.23023791250959325 No Hit CTCGCAGGGTCAAAGATCCTCTTCTGAGATGAGTTTTTGT 3 0.23023791250959325 No Hit CTCGCAGGGGAGGAGAAGCCCGGTTTACGCTGATGACCGA 3 0.23023791250959325 No Hit CTCGCAGGGAAGCAGTGGTATCAACGCAGAGTGCAGTTTT 2 0.15349194167306215 No Hit CTCGCAGGGGTATTAACTTAGCTTATGTATCTATGTATGT 2 0.15349194167306215 No Hit CTCGCAGGGCTCCTACTTGGATAACTGTGGTAATTCTAGA 2 0.15349194167306215 No Hit CTCGCAGGGGCGGCACCGGCGAGTCAGAGGCCCTGAGTAT 2 0.15349194167306215 No Hit CTCGCAGGGGGGGCTAGCAATAATTTTGTTTAACTTTAAG 2 0.15349194167306215 No Hit CTCGCAGGGATCTTGGAAAATTTTGCCAAAGACCATTGCC 2 0.15349194167306215 No Hit CTCGCAGGGTACCTGGTTGATCCTGCCAGTAGCATATGCC 2 0.15349194167306215 No Hit CTCGCAGGGGGGGGGAAAAAAAAAAAAAAAAAAAAAAAAA 2 0.15349194167306215 No Hit CTCGCAGGGGGCCGGTACAGTGAAACTGCGAATGGCTCAT 2 0.15349194167306215 No Hit CTCGCAGGGGGGCCTCTACCACTGAGCCACGCCTACTTCT 2 0.15349194167306215 No Hit CTCGCAGGGCAGCATCTCTCTGGAGGTTATCCTTCGGGAT 2 0.15349194167306215 No Hit CTCGCAGGGGAATTGTTTTATCCAACTCATCCCTTGACAT 2 0.15349194167306215 No Hit CTCGCAGGGGGGGGCTGGAGAGATGGCTCAGTGGTTAAGA 2 0.15349194167306215 No Hit CTCGCAGGGTCAAAGATCCTCTTCTGAAGGAGATTGGGCG 2 0.15349194167306215 No Hit CTCGCAGGGGCTTTCGGCCGCCGCAGCCATCTTCCAGTAA 2 0.15349194167306215 No Hit CTCGCAGGGTCCCAGATCATCTTCTGAGATGAGTTTTTGT 2 0.15349194167306215 No Hit CTCGCAGGGGCCCACTTCGCCATCATATTCGTAGGAGTAA 2 0.15349194167306215 No Hit CTCGCAGGGACTGTATGACTATACACATAATTTTCCTTTT 2 0.15349194167306215 No Hit CTCGCAGGGGGCTTTGGTGACTCTAGATAATATCGTATGC 2 0.15349194167306215 No Hit CTCGCAGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGG 2 0.15349194167306215 No Hit CTCGCAGGGAGACAAATGACAGCAATTCATGGTTCAAGTA 2 0.15349194167306215 No Hit CTCGCAGGGGGGGAGGTAGTGACGAAAAATAACAATACAG 2 0.15349194167306215 No Hit CTCGCAGGGTCACAGATCCTCTTCTGAAGGACATTGGGCG 2 0.15349194167306215 No Hit CTCGCAGGGACAGTAAGTCATTACTTTGATGTATTTTCAT 2 0.15349194167306215 No Hit CTCGCAGGGATGAAAACTGCCGAGGAGAACTTGGACCGCA 2 0.15349194167306215 No Hit CTCGCAGGGTAGATATGTTTTGTTTTCTCTTTTTTTTAAA 2 0.15349194167306215 No Hit CTCGCAGGGAAAGACACAGTTGTACACTGAATTTTGTATA 2 0.15349194167306215 No Hit CTCGCAGGGTGATTTAATATTACTACCCCTGACATTCACG 2 0.15349194167306215 No Hit CTCGCAGGGGGGGCTGGAGAGATGGCTCAGCGGTTAAGAG 2 0.15349194167306215 No Hit CTCGCAGGGAGGCTGGGTTTTGTCAGAGATTGATAAACTG 2 0.15349194167306215 No Hit CTCGCAGGGTCACAGATCCTCTCCTGAAGGAGATTGGGCG 2 0.15349194167306215 No Hit CTCGCAGGGGGAATCAGGGTTCGATTCCGGAGAGGGAGCC 2 0.15349194167306215 No Hit CTCGCAGGGAAATCAGTTATGGTTCCTTTGGTCGCTCGCT 2 0.15349194167306215 No Hit CTCGCAGGGCTCCAAGGAAGGCAGCAGGCGCGCAAATTAC 2 0.15349194167306215 No Hit CTCGCAGGGGGCTGACTGTGCCACCGTACCTCCTGCTATC 2 0.15349194167306215 No Hit CTCGCAGGGATTGGAGGGCAAGTCTGGTGCCAGCAGCCGC 2 0.15349194167306215 No Hit CTCGCAGGGAGTTATGGTTCCTTTGGTCGCTCGCTCCTCT 2 0.15349194167306215 No Hit CTCGCAGGGAACATTTCCTGGGCCTTTCAGGAATACCACG 2 0.15349194167306215 No Hit CTCGCAGGGAATTTTGTGAGGCTGTGTTGGAAATAACCCG 2 0.15349194167306215 No Hit CTCGCAGGGGTCTCAAAGATTAAGCCATGCATGTCTAAGT 2 0.15349194167306215 No Hit CTCGCAGGGTGCTATGTATTTTTTTAAATGTTCAATATGA 2 0.15349194167306215 No Hit CTCGCAGGGGCAAGAAAATTGACTAATGCTGCTCATTCCT 2 0.15349194167306215 No Hit CTCGCAGGGTAAGTTCAATTTTAAACTTGCTAAAAAAACA 2 0.15349194167306215 No Hit CTCGCAGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTT 2 0.15349194167306215 No Hit CTCGCAGGGAGTGATAATGAGGTTTAAGGGGATTTAGGAC 2 0.15349194167306215 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0 0.0 0.0 0.0 0.0 11 0.0 0.0 0.0 0.0 0.0 12 0.0 0.0 0.0 0.0 0.0 13 0.0 0.0 0.0 0.0 0.0 14 0.0 0.0 0.0 0.0 0.0 15 0.07674597083653108 0.0 0.0 0.0 0.0 16 0.07674597083653108 0.0 0.0 0.0 0.0 17 0.07674597083653108 0.0 0.0 0.0 0.0 18 0.07674597083653108 0.0 0.0 0.0 0.0 19 0.07674597083653108 0.0 0.0 0.0 0.0 20 0.07674597083653108 0.0 0.0 0.0 0.0 21 0.07674597083653108 0.0 0.0 0.0 0.0 22 0.07674597083653108 0.0 0.0 0.0 0.0 23 0.07674597083653108 0.0 0.0 0.0 0.0 24 0.07674597083653108 0.0 0.0 0.0 0.0 25 0.07674597083653108 0.0 0.0 0.0 0.0 26 0.07674597083653108 0.0 0.0 0.0 0.0 27 0.07674597083653108 0.0 0.0 0.0 0.0 28 0.07674597083653108 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position AGGGGTC 20 0.0019639186 33.73077 6 GCAGGGT 30 6.746417E-6 33.73077 4 GCAGGGG 55 1.8189894E-12 33.73077 4 GCAGGGC 20 0.0019639186 33.73077 4 CAGGGGT 20 0.0019639186 33.73077 5 CAGGGGG 20 0.0019639186 33.73077 5 CGCAGGG 120 0.0 33.73077 3 TCGCAGG 125 0.0 33.73077 2 CTCGCAG 130 0.0 33.73077 1 >>END_MODULE