FastQCFastQC Report
Fri 17 Jun 2016
SRR1526166_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526166_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences143709
Sequences flagged as poor quality0
Sequence length56
%GC53

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACGTTGGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAG55553.86545031974337No Hit
ACGTTGGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTCGTCTCAAAGATTAAG13460.93661496496392No Hit
ACGTTGGGGTACCTGGTTGATCCTGCCAGTAGCATATGCCTGTCTCAAAGATTAAG6470.4502153657738903No Hit
ACGTTGGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCCAAGATTAAG6120.4258605932822579No Hit
ACGTTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC5120.35627552902045106No Hit
ACGTTGGGGTCACAGATCCTCTTCTGAGATGAGTTTTTGTTCAATCCGCCCAATCT4990.34722947066641613No Hit
ACGTTGGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCCACGATTAAG4000.27834025704722737No Hit
ACGTTGGGGTCACAGATCCTCTTCTGAAGGAGATTGGGCGGATTGAACAAAAACTC3770.2623356922670118No Hit
ACGTTGGGGGGGCGCTGACCCCCCTTCCCGGGGGGGGATGCGTGCATTTATCAGAT3340.23241411463443487No Hit
ACGTTGGGGCTCCTACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCGAC3010.20945104342803858No Hit
ACGTTGGGGAACTGTGGTAATTCTAGAGCTAATACATGCCGACGGGCGCTGACCCC2670.18579212157902428No Hit
ACGTTGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTC2520.17535436193975326No Hit
ACGTTGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2300.16004564780215574No Hit
ACGTTGGGGTACCTGGTTGATCCTGCCAGTAGCATATGCCGTCTTCTGCTTGGATC2260.15726224523168347No Hit
ACGTTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCCCCCGACTGCTCTTCC2130.14821618687764856No Hit
ACGTTGGGGGGTTCCTTTGGTCGCTCGCTCCTCTCCTACTTGGATAACTGTGGTAA2000.13917012852361368No Hit
ACGTTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTTCTTCC1620.11272780410412708No Hit
ACGTTGGGGGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCCATTCGAACGTC1570.10924855089103674No Hit
ACGTTGGGGGGTGAGCTCCCTCCCGGCTCCGGCCGGGGGTCGGGCGCCGGCGGCTT1500.10437759639271027No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCATTG309.724208E-750.49876439
TAACGTG150.00809204850.49876438
CCCCCGA505.456968E-1250.4987640
ATATAAT150.00811469650.4630937
TATGCTG204.01377E-450.3918935
TCCGGAC150.00816013250.39188835
AGATTAC252.0558451E-550.09151549
GATTACG252.0601161E-550.0739650
CATATGT204.1699194E-450.00385333
CCTAGTT204.1915814E-449.951412
TCCAGCG150.00846009849.9339432
CAACTAG204.206072E-449.91649216
ACTAATC204.2133324E-449.8990610
ATGACCC150.00848350749.8990610
CGAGAGT150.00849522949.88163428
CCGTAGC204.235173E-449.8468327
TCTCTAA150.00851871149.84682527
CTCATAG150.00851871149.84682519
GCAACGA150.00854224149.81206524
GCCCTAC301.0688091E-649.81206526
Produced by FastQC (version 0.11.5)