##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1526166_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	143709
Sequences flagged as poor quality	0
Sequence length	56
%GC	53
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	36.619126150763	38.0	37.0	39.0	33.0	40.0
2	35.712091796616775	39.0	37.0	40.0	28.0	40.0
3	35.444739021216485	38.0	36.0	39.0	27.0	40.0
4	35.059898823316566	39.0	35.0	39.0	26.0	40.0
5	35.754858777112084	39.0	38.0	40.0	28.0	40.0
6	34.96787257583033	38.0	35.0	39.0	26.0	40.0
7	35.91857155780083	39.0	37.0	40.0	30.0	40.0
8	35.75901300544851	38.0	36.0	39.0	29.0	40.0
9	35.811841986236075	39.0	36.0	40.0	30.0	40.0
10	35.17671822919929	38.0	35.0	39.0	27.0	40.0
11	34.48213403475078	38.0	34.0	39.0	26.0	40.0
12	33.34848200182313	38.0	32.0	39.0	19.0	40.0
13	33.05956481500811	38.0	32.0	39.0	17.0	40.0
14	33.67582406112352	38.0	33.0	39.0	21.0	40.0
15	32.52176968735431	38.0	31.0	39.0	14.0	40.0
16	32.099249177156615	37.0	29.0	39.0	16.0	40.0
17	31.09388416870203	36.0	27.0	39.0	13.0	40.0
18	30.500511450222323	35.0	26.0	39.0	12.0	40.0
19	31.733294365697347	36.0	29.0	39.0	16.0	40.0
20	32.27021968004787	36.0	30.0	39.0	18.0	40.0
21	32.31183850698286	37.0	30.0	39.0	16.0	40.0
22	31.561196584765046	36.0	28.0	39.0	13.0	40.0
23	32.33315241216625	37.0	30.0	39.0	16.0	40.0
24	31.719286892261444	36.0	29.0	39.0	14.0	40.0
25	32.06353116367103	36.0	30.0	39.0	16.0	40.0
26	32.62641866549764	38.0	31.0	39.0	16.0	40.0
27	32.75150477701466	38.0	32.0	39.0	17.0	40.0
28	32.60767940769193	37.0	31.0	39.0	17.0	40.0
29	32.21649305193133	37.0	31.0	39.0	14.0	40.0
30	31.879144660390093	36.0	30.0	39.0	12.0	40.0
31	32.654684118600784	37.0	32.0	39.0	13.0	40.0
32	32.41513057637309	37.0	32.0	39.0	11.0	40.0
33	32.23180872457536	37.0	31.0	39.0	10.0	40.0
34	32.08396829704472	36.0	31.0	39.0	10.0	40.0
35	31.93792316417204	36.0	31.0	39.0	4.0	40.0
36	31.07661315575225	36.0	30.0	38.0	4.0	40.0
37	30.791578815523035	35.0	29.0	38.0	4.0	40.0
38	30.097224251786596	35.0	28.0	38.0	4.0	40.0
39	29.17610588063378	35.0	26.0	38.0	4.0	40.0
40	27.11318010702183	33.0	17.0	38.0	4.0	39.0
41	25.236422214335914	31.0	11.0	37.0	4.0	39.0
42	26.137681008148412	31.0	17.0	37.0	4.0	39.0
43	26.045898308387088	31.0	15.0	37.0	4.0	39.0
44	25.046329735785513	30.0	11.0	36.0	4.0	39.0
45	26.08333507295994	32.0	16.0	37.0	4.0	39.0
46	23.97542255530273	28.0	7.0	36.0	4.0	39.0
47	23.039461689942872	27.0	4.0	35.0	4.0	39.0
48	23.732528929990465	28.0	4.0	35.0	4.0	39.0
49	22.66815578704187	27.0	4.0	35.0	4.0	39.0
50	23.261911223375016	28.0	4.0	35.0	4.0	39.0
51	23.464605557063233	29.0	4.0	35.0	4.0	39.0
52	23.411470401992915	29.0	4.0	36.0	4.0	39.0
53	23.210056433487118	29.0	4.0	36.0	4.0	39.0
54	22.78402883605063	28.0	4.0	35.0	4.0	38.0
55	22.423773041354405	28.0	4.0	35.0	4.0	38.0
56	22.031494200084893	27.0	4.0	35.0	4.0	38.0
>>END_MODULE
>>Per tile sequence quality	fail
#Tile	Base	Mean
1	1	3.4590058737887333
1	2	4.709178659960109
1	3	5.034225559301213
1	4	5.359365940665008
1	5	4.761954278883856
1	6	5.319196142200411
1	7	4.379824627147649
1	8	4.506440718152888
1	9	4.5398591585392225
1	10	5.029707512384135
1	11	-0.2799666885802523
1	12	-6.692929292106804
1	13	-17.913756285000336
1	14	-5.754552777393574
1	15	1.5992607121450142
1	16	-0.54673980611188
1	17	-5.3221979877217365
1	18	-3.455010153980165
1	19	0.7507299821042714
1	20	0.08108498338559755
1	21	6.99610804656346
1	22	7.748337106515468
1	23	6.913847533338348
1	24	7.472875012614672
1	25	6.796500082625052
1	26	7.046959876427909
1	27	6.9228834893203235
1	28	6.679342000747006
1	29	7.036161225317308
1	30	7.477526131750029
1	31	5.894695041270548
1	32	5.664808199185973
1	33	6.191465952191084
1	34	5.768593154400406
1	35	6.725029841647316
1	36	5.557870808373956
1	37	5.764765920921381
1	38	6.355641222872681
1	39	6.366902836016546
1	40	-7.496485960516882
1	41	1.986391388253029
1	42	0.6386642370130104
1	43	-7.313419855992606
1	44	-13.189886770319687
1	45	-0.3799422872210485
1	46	-2.2943958451722395
1	47	-7.150941022089981
1	48	-2.1755524549214194
1	49	-11.457304152932451
1	50	-1.0017780427725675
1	51	5.573989566526652
1	52	5.263818754105536
1	53	5.396052040333121
1	54	5.644272835067056
1	55	5.279260610188473
1	56	8.894832542233907
2	1	3.6678214464755072
2	2	4.8201767682936065
2	3	5.287220697921718
2	4	5.37112274964737
2	5	4.929905787131929
2	6	5.491378474442136
2	7	4.625842974278626
2	8	4.783132539237847
2	9	4.714970522776554
2	10	5.039959124368757
2	11	4.200781028198627
2	12	0.0037938533481138847
2	13	1.911152691262302
2	14	5.296583239529724
2	15	6.227009222203389
2	16	5.164872585320705
2	17	7.613932842374069
2	18	5.210897134020975
2	19	6.4207867320991845
2	20	6.140804691296019
2	21	7.657072593072012
2	22	8.643217402673148
2	23	7.004891611201778
2	24	7.595772313430324
2	25	7.216897535994455
2	26	6.601563948595999
2	27	7.089512865070379
2	28	6.559251688927141
2	29	7.260333875481045
2	30	7.232443447617651
2	31	6.575267829749688
2	32	6.095864074539396
2	33	5.891545280140964
2	34	5.693496028513046
2	35	5.706113176675689
2	36	6.565722241362181
2	37	6.463523116373242
2	38	7.110395814740546
2	39	7.132376871368333
2	40	4.928671169845384
2	41	9.888797669399416
2	42	8.992751660481883
2	43	7.844137656771057
2	44	-3.466639612904558
2	45	7.911821844341308
2	46	6.953025996456628
2	47	4.222429195349964
2	48	6.676307683800793
2	49	-0.3851970805406104
2	50	7.126489150035031
2	51	9.4402914602272
2	52	9.177636055734812
2	53	9.387936180845294
2	54	9.803193161328675
2	55	9.438872513449056
2	56	9.968404130623547
3	1	3.5848269430495634
3	2	4.954127525013739
3	3	5.251154136668802
3	4	5.16769303802927
3	5	4.978113753399533
3	6	5.405522740687147
3	7	4.691124203101758
3	8	4.729522118335808
3	9	4.533602402906062
3	10	4.9161920887539665
3	11	5.02969074885219
3	12	5.35346631777097
3	13	6.381392131063521
3	14	5.501537685731066
3	15	0.7377200120535505
3	16	6.849930186404958
3	17	7.8255321195369305
3	18	8.34595849988198
3	19	7.222439469091885
3	20	6.481912288615497
3	21	7.745526022783633
3	22	8.453604421722918
3	23	7.598182779035525
3	24	8.008918913836922
3	25	7.477758916914567
3	26	6.576622302635609
3	27	6.17248854461258
3	28	5.78666302843471
3	29	6.459396446070613
3	30	6.389453461923804
3	31	5.846199988472673
3	32	5.286700607932961
3	33	6.045336410326943
3	34	5.233214423212246
3	35	6.362803184958199
3	36	7.298121910061823
3	37	7.169625819482949
3	38	7.3818738660921035
3	39	8.424128245511849
3	40	10.092438958415517
3	41	12.151748501415078
3	42	10.748022347930114
3	43	11.077346752471684
3	44	11.452812988556179
3	45	11.104841948249142
3	46	12.818849353131576
3	47	12.97244801923371
3	48	12.427361821290436
3	49	12.510123301960707
3	50	12.525348422680164
3	51	12.259280994147836
3	52	12.098274561871396
3	53	12.268630405677758
3	54	12.526745604668786
3	55	12.492821764258487
3	56	11.21947709199694
4	1	3.6635296009819456
4	2	5.120605952842965
4	3	5.32584730736378
4	4	5.67584377969029
4	5	5.037201924470985
4	6	5.650176757703939
4	7	4.638718510759311
4	8	4.8088836121992244
4	9	4.64630099487956
4	10	5.2073410986176825
4	11	5.8102230882844665
4	12	6.853579261073438
4	13	6.795272862936123
4	14	6.412463067855903
4	15	7.145464157825703
4	16	8.319379023088946
4	17	9.356422112760335
4	18	9.644373528870759
4	19	8.240529221369574
4	20	7.8661265797080375
4	21	8.159218515818797
4	22	9.179698089368426
4	23	8.090728521073025
4	24	8.509935403559084
4	25	8.405738737711197
4	26	7.1809630902269
4	27	6.763332607559651
4	28	6.366118641716845
4	29	7.213123575051867
4	30	7.159482074227096
4	31	6.137499589406342
4	32	6.430628023037247
4	33	6.410858584861995
4	34	6.380191307483003
4	35	6.607400730323757
4	36	7.209499065396514
4	37	7.515025262295989
4	38	8.054601823324237
4	39	7.780445540896235
4	40	3.3964823286436676
4	41	11.652746167253497
4	42	10.812494149752265
4	43	11.436412334882647
4	44	12.08700845576497
4	45	10.529847595414271
4	46	13.58392728401028
4	47	13.793244645993738
4	48	13.311500816848
4	49	13.64055399242076
4	50	13.73593121012087
4	51	13.187072576107028
4	52	13.10467468234425
4	53	13.370768798871044
4	54	13.318214620556144
4	55	12.983936891131457
4	56	12.045657349507668
5	1	3.6453223445489087
5	2	4.856515653926323
5	3	5.003221529821289
5	4	5.380557247009868
5	5	5.0214903667390445
5	6	5.3544175548149795
5	7	4.681618058886592
5	8	4.5387809482299275
5	9	4.6065021633203145
5	10	5.106643437389877
5	11	5.574303933796934
5	12	6.238465158485095
5	13	6.855018377295842
5	14	6.194713356184728
5	15	7.404676500190188
5	16	8.196730557376437
5	17	9.100688623130715
5	18	9.778971097455205
5	19	8.194056286465713
5	20	8.0303511169639
5	21	7.99452130835439
5	22	8.983842461756918
5	23	7.855660172960867
5	24	8.476111123435807
5	25	8.186816803922838
5	26	7.100136484566203
5	27	7.2547205563600095
5	28	6.708237338659615
5	29	7.324049819592524
5	30	7.719482830499427
5	31	5.786400158501269
5	32	6.179621235493055
5	33	6.182634501369531
5	34	6.2832182874985065
5	35	6.65444086947787
5	36	8.385918494032765
5	37	8.259953510958336
5	38	9.037604602625063
5	39	9.644373241261132
5	40	10.835309350254146
5	41	12.929957791159268
5	42	12.118047079362217
5	43	11.867071615289518
5	44	12.619537619516837
5	45	11.79380554667252
5	46	13.726106482739738
5	47	13.965183161053012
5	48	13.305772053920474
5	49	13.719471010438948
5	50	13.719123057505112
5	51	12.875866510802908
5	52	12.713700792646573
5	53	13.020350013066274
5	54	12.630347119469004
5	55	13.079227205173545
5	56	12.058684140455089
6	1	3.5582531556398607
6	2	4.89935257859792
6	3	5.174389827706349
6	4	5.236657870925988
6	5	5.009450579627995
6	6	5.277893146339288
6	7	4.568475953518522
6	8	4.457631985764564
6	9	4.632415612400386
6	10	4.878306084149699
6	11	5.515619938341757
6	12	6.178186489240371
6	13	6.845609801937535
6	14	6.281882212205623
6	15	7.347646978791662
6	16	8.166969957779099
6	17	8.971340645376415
6	18	9.48196310415284
6	19	8.312480748761647
6	20	7.660506398324159
6	21	7.703234207272001
6	22	8.86784987699004
6	23	8.055791733548503
6	24	8.435925343939324
6	25	7.81121132621837
6	26	7.2140161265619405
6	27	6.437948574778492
6	28	6.838163385662625
6	29	6.997560294605975
6	30	7.02482299435216
6	31	5.9456482698094995
6	32	6.240776975322024
6	33	6.290434449542634
6	34	6.162880216655125
6	35	6.034351966414103
6	36	7.662414995717143
6	37	7.882434424706414
6	38	8.169005722883401
6	39	8.374215567890193
6	40	5.73687811059461
6	41	12.240321177449054
6	42	12.096299715557326
6	43	11.723830042143824
6	44	12.42532628537288
6	45	11.420512345962887
6	46	12.78550808140021
6	47	13.585371731228626
6	48	12.933216389838503
6	49	13.807198002286182
6	50	13.090542516512027
6	51	11.909753328830696
6	52	12.025479451924586
6	53	12.252713917625048
6	54	12.43906599841333
6	55	12.817700703688509
6	56	11.64707890195169
7	1	3.3310437884806774
7	2	4.680742641002759
7	3	5.005789540343869
7	4	5.2171858458782765
7	5	4.714560913954948
7	6	4.902134530826004
7	7	4.270819887811157
7	8	4.27421323000123
7	9	3.9521814334207335
7	10	4.65056059295285
7	11	5.497284496253876
7	12	5.961099143912154
7	13	6.801883525426206
7	14	6.160139165734385
7	15	7.220113792713729
7	16	7.761317065926033
7	17	8.388702486211912
7	18	9.554468519005617
7	19	7.741251309118493
7	20	7.408099201395075
7	21	7.707008520497109
7	22	8.48767359940646
7	23	7.539439950399959
7	24	7.842543259060172
7	25	7.071381599212728
7	26	6.601462246096151
7	27	6.164589650457767
7	28	5.973180863306247
7	29	6.642796296407354
7	30	6.378000065399316
7	31	4.847301676341633
7	32	4.963386398238111
7	33	5.144072587262176
7	34	4.697503107007037
7	35	4.198963490936418
7	36	6.259292609321825
7	37	6.793201939878724
7	38	6.84379288164045
7	39	7.708135063504695
7	40	8.114888447066054
7	41	11.029045416689044
7	42	10.596010208576988
7	43	10.435395309884171
7	44	11.103952092239556
7	45	10.231432120361891
7	46	11.677168135870417
7	47	12.711618219616177
7	48	12.04245702375157
7	49	12.277293003465655
7	50	11.249406791350655
7	51	10.948397149465041
7	52	11.169032024247716
7	53	11.888943035593787
7	54	11.118206484356154
7	55	11.340871984596056
7	56	10.695780409532485
8	1	3.496273241723074
8	2	4.712022261855843
8	3	5.044051013005991
8	4	5.335322477481554
8	5	4.873085939963502
8	6	5.303960909279361
8	7	4.570970159456053
8	8	4.661914073834311
8	9	4.502429959602679
8	10	4.7130042410860185
8	11	5.524448985908251
8	12	6.697753865909615
8	13	6.556039114375459
8	14	6.3409043951876285
8	15	6.684938668450638
8	16	7.750636219781082
8	17	8.784517998869411
8	18	9.23258665563722
8	19	7.733714608159065
8	20	7.366786058405829
8	21	7.638230344563691
8	22	8.28469128522313
8	23	7.302033870178015
8	24	7.70262879830419
8	25	7.736252712378835
8	26	6.182805443414384
8	27	6.30094382916543
8	28	5.862465339089397
8	29	6.100153827328633
8	30	6.711811185501045
8	31	5.352468709054616
8	32	5.735181566426757
8	33	5.84940145097735
8	34	5.1018494253517375
8	35	5.3854390428957295
8	36	5.973175397438801
8	37	5.757922781399937
8	38	6.691972282865745
8	39	7.613880064082668
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115	21	5.899458267187743
115	22	6.806810713313961
115	23	5.633572978672554
115	24	5.797666645234379
115	25	5.411273738451172
115	26	5.180282314734821
115	27	4.698925326221779
115	28	4.754811848761392
115	29	4.927908242803824
115	30	4.9969247262916205
115	31	3.524209667843518
115	32	3.3178560828270953
115	33	3.7338421574533847
115	34	3.723716540574614
115	35	3.71966948864846
115	36	5.271745624520385
115	37	5.203170889053428
115	38	5.6620311558682666
115	39	6.807824555251717
115	40	8.584997942081582
115	41	9.070653522588703
115	42	8.014477278359635
115	43	6.04428565144325
115	44	-1.0419541015829665
115	45	6.275622347523193
115	46	1.8163085182858438
115	47	-7.27498089788668
115	48	1.759273497031515
115	49	-2.446289281221386
115	50	1.8971270070721786
115	51	2.5495411272391877
115	52	2.8516925896995886
115	53	3.3179347008985722
115	54	2.878167262261041
115	55	3.089131504125394
115	56	5.229821919583145
116	1	3.5113303402709377
116	2	4.50508584567087
116	3	4.971162249436269
116	4	4.951563842207342
116	5	4.703876860589467
116	6	4.867671609970181
116	7	4.39815188792074
116	8	4.189617443353555
116	9	4.210790314882537
116	10	4.597030746090951
116	11	3.9978734889394048
116	12	4.013642770463395
116	13	5.174592617829568
116	14	4.919446619916236
116	15	-3.158512621553591
116	16	4.854569675300606
116	17	6.151132971377514
116	18	6.063659544400792
116	19	5.827271636214892
116	20	5.304217328672287
116	21	6.738047065274248
116	22	7.748528871575868
116	23	6.5959010631852095
116	24	7.285767103675681
116	25	7.192138797251246
116	26	6.079943466817742
116	27	5.817960606269619
116	28	5.756924665185551
116	29	5.512560425383057
116	30	6.045041130517252
116	31	4.819605322617289
116	32	4.755567862775269
116	33	4.924912518226751
116	34	4.373666709998574
116	35	4.848910070669586
116	36	6.402979429583169
116	37	6.2490548834724
116	38	7.155274129757043
116	39	7.573022880944361
116	40	9.443518970584673
116	41	8.902864006941904
116	42	7.0356453070620475
116	43	2.896508095134699
116	44	2.4964951708906256
116	45	6.513573314236368
116	46	2.29344624999802
116	47	-3.8813193471591525
116	48	1.9068517310358999
116	49	-0.08117068445040942
116	50	2.873766433023942
116	51	6.450438077607934
116	52	6.182647343536541
116	53	6.038684152762237
116	54	5.5930167739213985
116	55	6.141792456886005
116	56	7.494043565985379
117	1	2.348370802698689
117	2	3.1600222618558433
117	3	3.635855891054767
117	4	3.5355175994327794
117	5	3.547622525329352
117	6	3.9903999336696074
117	7	3.2650189399438574
117	8	3.027767732370897
117	9	3.3293567888709745
117	10	3.6037359484030915
117	11	-0.013502233603944092
117	12	-5.181270524334291
117	13	-7.619765763673321
117	14	-2.0653395072513945
117	15	-7.397402794963995
117	16	-8.073754024121357
117	17	0.46900580374745715
117	18	-3.323901149240829
117	19	0.03342192523223275
117	20	-0.0937017464722203
117	21	5.095596198222228
117	22	5.785569334003615
117	23	4.934814357982894
117	24	5.772287334889555
117	25	5.057618566037377
117	26	5.258903004389996
117	27	4.6898218779459135
117	28	4.581294607382077
117	29	4.967080656596927
117	30	6.120786795257143
117	31	3.9487613919814457
117	32	3.561718151792615
117	33	4.846669743660282
117	34	4.59433723022979
117	35	4.057048798993289
117	36	6.27229734865832
117	37	6.177239854570669
117	38	6.538996673109647
117	39	7.404514210424129
117	40	9.316860011047101
117	41	7.050239729485256
117	42	5.840408312842396
117	43	-4.566197107177441
117	44	0.6303217604860016
117	45	4.678105106143882
117	46	-0.2565190679210545
117	47	0.8455708262512509
117	48	1.1402390142728933
117	49	-0.2118065226006962
117	50	1.8185063174170075
117	51	5.8383687134460835
117	52	6.148520175906484
117	53	5.26027952848478
117	54	5.481201745019664
117	55	5.890786676539186
117	56	8.439477091996938
118	1	2.2618946122224983
118	2	3.0548794047129846
118	3	3.49376065295953
118	4	3.6284699803851552
118	5	3.236384430091263
118	6	3.3968761241457983
118	7	2.8490189399438606
118	8	3.142053446656611
118	9	2.7567853602995456
118	10	3.0170692817364255
118	11	-1.864168900270613
118	12	-8.950794333858099
118	13	-9.836527668435227
118	14	-11.573149031060918
118	15	-9.242355175916376
118	16	-8.64518259554993
118	17	-0.4730894343477807
118	18	-6.490758292097972
118	19	-1.7441018842915739
118	20	-1.7470350798055563
118	21	3.671215245841278
118	22	5.003855048289328
118	23	4.198433405601946
118	24	4.527715906318122
118	25	4.035904280323088
118	26	4.634141099628085
118	27	4.0582028303268665
118	28	4.046437464524935
118	29	3.9449854185016875
118	30	4.3489772714476125
118	31	3.62076139198145
118	32	4.222289580364038
118	33	3.583431648422181
118	34	4.558527706420264
118	35	4.037239275183762
118	36	4.987344967705937
118	37	4.2747636640944755
118	38	5.416710958823931
118	39	5.5332761151860375
118	40	7.337050487237576
118	41	6.183954015199543
118	42	5.742884503318585
118	43	0.08599336901303545
118	44	1.4444169985812394
118	45	4.681533677572453
118	46	-2.099947639349626
118	47	-0.10223869755827408
118	48	-0.990808604774724
118	49	-0.47847318926736193
118	50	0.17660155551224577
118	51	4.16103538011275
118	52	4.1108058901922
118	53	3.3330414332466844
118	54	4.752058887876807
118	55	4.035929533682044
118	56	6.595667568187416
119	1	2.4195349818031673
119	2	3.313514799169276
119	3	4.1064827567264075
119	4	4.056472823313371
119	5	3.4525180477174118
119	6	4.081026799341245
119	7	3.4311084921826662
119	8	3.266573702520148
119	9	3.433117982900825
119	10	3.8703031125821923
119	11	0.1935425425154591
119	12	-6.600972016871605
119	13	-2.5646015845688446
119	14	-6.856802193818556
119	15	-6.982298317352054
119	16	-7.620022680837774
119	17	0.26052819180716114
119	18	-6.209095179091573
119	19	-0.3942795673050803
119	20	-0.6181793584125188
119	21	4.581805153446108
119	22	5.350763363854366
119	23	4.822456149027673
119	24	3.9712127080238773
119	25	5.200723043649319
119	26	5.9478582282705865
119	27	5.968508445110093
119	28	5.783324458128348
119	29	6.518901552119317
119	30	6.435473362421316
119	31	5.017776317354585
119	32	5.07133009209112
119	33	5.221953325749833
119	34	4.942038722767101
119	35	5.5416458139186595
119	36	7.069789885971751
119	37	6.926433884421417
119	38	7.299653389527553
119	39	7.764335105946518
119	40	10.714591354330683
119	41	7.707194953365853
119	42	7.4315127904543345
119	43	-1.033480689266991
119	44	2.4197545963068947
119	45	6.336970777785673
119	46	-1.0837429485180685
119	47	0.3379887366990104
119	48	-3.650865463339045
119	49	-0.6759856270783082
119	50	1.4041779592080523
119	51	6.309294086580412
119	52	6.045773907249767
119	53	6.2402198269922415
119	54	5.606216670392797
119	55	6.916100109375005
119	56	9.450820375579028
120	1	2.209513659841541
120	2	3.1993238491574303
120	3	3.7064590656579455
120	4	3.9586287105438913
120	5	3.409400303107134
120	6	3.9508443781140485
120	7	2.9839395748644932
120	8	3.16268836729153
120	9	2.9059917095058907
120	10	3.4694502341173745
120	11	-0.883216519318232
120	12	-9.676191159254927
120	13	-5.131765763673322
120	14	-6.658863316775204
120	15	-0.8598154933766935
120	16	-8.953119103486436
120	17	0.32532326406491663
120	18	-6.987583688923369
120	19	-0.4456891858788765
120	20	-0.5454477782182536
120	21	3.993437468063501
120	22	5.510204254638534
120	23	4.595258802427338
120	24	4.932477811080027
120	25	4.053364597783407
120	26	5.60398236946936
120	27	5.5296314017554415
120	28	5.795643813731289
120	29	6.016413989930264
120	30	6.26643758890793
120	31	5.3969518681719215
120	32	5.3556229136973705
120	33	5.275495140485678
120	34	5.8109086588012175
120	35	5.272159910104403
120	36	5.7175036978646645
120	37	5.62555731488813
120	38	6.5309966731096445
120	39	7.158672940582857
120	40	8.262447312634404
120	41	-4.057315826070301
120	42	-1.5094964490623681
120	43	-7.19019710717744
120	44	-13.306376652212412
120	45	-3.575609179570403
120	46	-6.763439702841689
120	47	-5.5292228245423996
120	48	-4.916205430171548
120	49	-12.980060490854665
120	50	-3.7154619365512467
120	51	0.7943687134460831
120	52	0.4996947790810893
120	53	0.747327147532399
120	54	1.1203128561307736
120	55	1.1724374701899798
120	56	4.306778679298528
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
4	6407.0
5	47.0
6	111.0
7	576.0
8	875.0
9	713.0
10	586.0
11	511.0
12	416.0
13	406.0
14	500.0
15	602.0
16	717.0
17	937.0
18	1164.0
19	1435.0
20	1794.0
21	2239.0
22	2781.0
23	3180.0
24	3563.0
25	4069.0
26	4317.0
27	4631.0
28	4811.0
29	5212.0
30	5756.0
31	6469.0
32	7386.0
33	8242.0
34	9089.0
35	9808.0
36	10779.0
37	11514.0
38	11730.0
39	10332.0
40	4.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.0	100.0	0.0	0.0
2	0.0	0.0	0.0	100.0
3	100.0	0.0	0.0	0.0
4	0.0	0.0	100.0	0.0
5	0.0	0.0	100.0	0.0
6	100.0	0.0	0.0	0.0
7	99.50594604374116	0.40776847657418813	0.054276350124209345	0.03200912956043115
8	99.422443966627	0.38898050922350025	0.11899045988768972	0.06958506426180684
9	96.50046971225775	1.5079503148811801	1.273442121011795	0.7181378518492746
10	51.07682886945146	23.986667501687435	17.029552776791988	7.906950852069112
11	34.25324788287442	31.410698007779608	17.03999053643126	17.29606357291471
12	28.7567236568343	17.637030387797566	19.95073377450264	33.6555121808655
13	22.23103633036205	15.74292493859118	22.839209792010244	39.18682893903653
14	20.890132142037032	22.040373254284702	33.542088526118754	23.52740607755951
15	34.251164482242515	18.50792407435901	22.335678616888842	24.90523282650963
16	36.81539393348696	22.04036451987942	20.714141505559	20.430100041074624
17	26.110469243725554	20.142958560113588	34.06088614819249	19.685686047968375
18	25.00887209569338	20.09477485752458	30.53879715257917	24.357555894202868
19	33.59929263181346	23.492143061038355	19.33008890838329	23.57847539876489
20	25.867714472822527	31.537989939946215	22.056180244952557	20.5381153422787
21	23.98213030680482	22.451933447918336	30.576798624979993	22.989137620296855
22	22.4947637239143	19.96534663799762	25.755519835222568	31.784369802865513
23	22.940108135189863	21.343826761024015	23.648484089375057	32.06758101441107
24	23.180397915086395	21.59096444651668	30.40661382473086	24.822023813666068
25	32.856148653350964	22.139327719395922	21.77674159649245	23.227782030760665
26	26.13248666638816	21.744579521243264	20.90557156981521	31.21736224255337
27	22.179196991329178	23.317895323327644	19.258279068147786	35.24462861719539
28	23.96331322241011	32.39250398391126	20.659276424291768	22.98490636938686
29	36.51780548837015	22.93001177971241	18.702558776582766	21.84962395533468
30	26.35034920723343	21.515065001984528	31.17867015757846	20.955915633203585
31	27.849035068794407	31.24804264766266	20.512356547822034	20.390565735720898
32	37.85125117460759	22.40629241638534	19.269133052587616	20.47332335641945
33	27.937375018290005	22.721413591042424	20.126671358198454	29.214540032469117
34	23.493354161145902	30.660120782716632	25.71932662938116	20.1271984267563
35	23.305010908431903	25.059421330340776	33.20206040413475	18.433507357092573
36	24.72444453728267	33.52272331655282	21.676797638195502	20.076034507969002
37	26.589345582034003	22.831094785705822	30.36083947278544	20.21872015947474
38	34.56494747562115	25.229140218467933	20.019251963560777	20.186660342350134
39	26.423310874366816	22.586938496805345	19.90022366064896	31.08952696817887
40	21.25765515499716	18.450940365202626	27.569080539596353	32.72232394020386
41	18.611208775754164	16.81667955839955	24.939174460305182	39.632937205541104
42	29.416938315382414	23.31409106456049	22.377339261906588	24.89163135815051
43	19.519343493552167	19.301624518506113	31.5448277787082	29.634204209233516
44	20.951169390351307	21.402709239092445	22.98030279731033	34.665818573245915
45	24.10320078676999	21.797992620543905	31.38152067014947	22.717285922536636
46	20.807650692820495	24.90593696555094	23.202680534710833	31.083731806917736
47	18.481765096681702	27.255498444001535	23.02597992936816	31.2367565299486
48	22.594392835754377	29.56783331708472	25.173744098271612	22.66402974888929
49	21.515198035001536	28.66692717782678	22.25416585256929	27.563708934602392
50	29.648949078787712	22.74160030125943	24.220700428178915	23.388750191773944
51	21.572817161052456	29.96061672532261	26.40425115915312	22.062314954471816
52	21.252579619610685	23.580512019632994	32.2027162697306	22.964192091025712
53	21.435699879264984	21.865600290322355	33.530836281919754	23.167863548492907
54	21.60949536675226	29.10921625544267	26.686530088199174	22.594758289605895
55	20.946337445112288	28.823640285660435	25.50419904081761	24.72582322840967
56	28.429374904051468	21.273359105689924	26.51668457705888	23.780581413199727
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	1.5
5	3.0
6	2.5
7	2.0
8	31.5
9	61.0
10	231.5
11	402.0
12	446.5
13	456.5
14	422.0
15	398.0
16	374.0
17	372.0
18	370.0
19	353.5
20	337.0
21	324.5
22	338.0
23	364.0
24	396.5
25	429.0
26	475.5
27	522.0
28	599.0
29	695.0
30	714.0
31	827.5
32	941.0
33	1077.5
34	1214.0
35	1413.0
36	1612.0
37	1755.5
38	2045.0
39	2191.0
40	2361.0
41	2531.0
42	2956.5
43	3382.0
44	4428.5
45	5475.0
46	9438.5
47	11905.5
48	10409.0
49	8978.0
50	7547.0
51	7464.5
52	7382.0
53	7777.5
54	8369.0
55	8565.0
56	8438.0
57	8311.0
58	8695.5
59	9080.0
60	9604.0
61	10128.0
62	9731.0
63	8180.5
64	7027.0
65	6436.5
66	5846.0
67	4913.0
68	3980.0
69	3621.0
70	3262.0
71	2813.0
72	2095.0
73	1826.0
74	1432.5
75	1039.0
76	780.5
77	522.0
78	422.0
79	339.0
80	356.0
81	224.0
82	92.0
83	53.5
84	15.0
85	9.0
86	3.0
87	1.5
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.002783402570472274
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.5058834171833357
16	0.047317843698028654
17	0.02157136992116012
18	0.0
19	0.054276350124209345
20	0.11899045988768972
21	0.001391701285236137
22	0.0
23	0.0
24	0.006958506426180684
25	0.0132211622097433
26	0.060539005907771955
27	0.08628547968464048
28	0.004175103855708411
29	0.16839585551357258
30	0.0681933629765707
31	0.013917012852361368
32	0.030617428275195008
33	0.13081992081219687
34	0.21640954985421929
35	0.16839585551357258
36	0.06262655783562616
37	0.16561245294310029
38	0.24146017298846975
39	0.13081992081219687
40	0.8057950441517232
41	1.0430801132844847
42	0.3117410878928947
43	0.2818195102603177
44	0.44743196320341805
45	0.23519751720490711
46	0.3159161917486031
47	0.49683735882930086
48	0.07445601876013333
49	0.27903610768984544
50	0.21640954985421929
51	0.3486211719516523
52	0.19275062800520495
53	0.2922572698995888
54	0.27625270511937317
55	0.3214829968895476
56	0.2804278089750816
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
56	143709.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	67.60258578098797
#Duplication Level	Percentage of deduplicated	Percentage of total
1	88.77829358421427	60.016422075165785
2	6.663853177013103	9.00987412061875
3	1.8744017045629997	3.801432060622508
4	0.788463319986413	2.1320863689817617
5	0.4611378163889204	1.5587054394644733
6	0.2768885549299544	1.1231029371855625
7	0.21204104950026248	1.0034166266552549
8	0.1266070344103509	0.6847170323361793
9	0.11734310506325205	0.7139427593261383
>10	0.6093606859425018	7.5339749076258276
>50	0.05661290156560406	2.5739515270442355
>100	0.029850439007318503	3.813957372189633
>500	0.003087976449032949	1.232351488076599
>1k	0.001029325483010983	0.93661496496392
>5k	0.001029325483010983	3.86545031974337
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
ACGTTGGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAG	5555	3.86545031974337	No Hit
ACGTTGGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTCGTCTCAAAGATTAAG	1346	0.93661496496392	No Hit
ACGTTGGGGTACCTGGTTGATCCTGCCAGTAGCATATGCCTGTCTCAAAGATTAAG	647	0.4502153657738903	No Hit
ACGTTGGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCCAAGATTAAG	612	0.4258605932822579	No Hit
ACGTTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC	512	0.35627552902045106	No Hit
ACGTTGGGGTCACAGATCCTCTTCTGAGATGAGTTTTTGTTCAATCCGCCCAATCT	499	0.34722947066641613	No Hit
ACGTTGGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCCACGATTAAG	400	0.27834025704722737	No Hit
ACGTTGGGGTCACAGATCCTCTTCTGAAGGAGATTGGGCGGATTGAACAAAAACTC	377	0.2623356922670118	No Hit
ACGTTGGGGGGGCGCTGACCCCCCTTCCCGGGGGGGGATGCGTGCATTTATCAGAT	334	0.23241411463443487	No Hit
ACGTTGGGGCTCCTACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCGAC	301	0.20945104342803858	No Hit
ACGTTGGGGAACTGTGGTAATTCTAGAGCTAATACATGCCGACGGGCGCTGACCCC	267	0.18579212157902428	No Hit
ACGTTGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTC	252	0.17535436193975326	No Hit
ACGTTGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	230	0.16004564780215574	No Hit
ACGTTGGGGTACCTGGTTGATCCTGCCAGTAGCATATGCCGTCTTCTGCTTGGATC	226	0.15726224523168347	No Hit
ACGTTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCCCCCGACTGCTCTTCC	213	0.14821618687764856	No Hit
ACGTTGGGGGGTTCCTTTGGTCGCTCGCTCCTCTCCTACTTGGATAACTGTGGTAA	200	0.13917012852361368	No Hit
ACGTTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTTCTTCC	162	0.11272780410412708	No Hit
ACGTTGGGGGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCCATTCGAACGTC	157	0.10924855089103674	No Hit
ACGTTGGGGGGTGAGCTCCCTCCCGGCTCCGGCCGGGGGTCGGGCGCCGGCGGCTT	150	0.10437759639271027	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	6.958506426180685E-4	0.0	0.0	0.0	0.0
8	6.958506426180685E-4	0.0	0.0	0.0	0.0
9	0.001391701285236137	0.0	0.0	0.0	0.0
10	0.004870954498326479	0.0	0.0	0.0	0.0
11	0.008350207711416821	0.0	0.0	0.0	0.0
12	0.009046058354034889	0.0	0.0	0.0	0.0
13	0.009741908996652959	0.0	0.0	0.0	0.0
14	0.014612863494979438	0.0	0.0	0.0	0.0
15	0.016700415422833643	0.0	0.0	0.0	0.0
16	0.01878796735068785	0.0	0.0	0.0	0.0
17	0.019483817993305917	0.0	0.0	0.0	0.0
18	0.021571369921160124	0.0	0.0	0.0	0.0
19	0.0264423244194866	0.0	0.0	0.0	0.0
20	0.028529876347340808	0.0	0.0	0.0	0.0
21	0.029921577632576944	0.0	0.0	0.0	0.0
22	0.03200912956043115	0.0	0.0	0.0	0.0
23	0.03479253213090342	0.0	0.0	0.0	0.0
24	0.03688008405875763	0.0	0.0	0.0	0.0
25	0.04035933727184797	0.0	0.0	0.0	0.0
26	0.04314273984232025	0.0	0.0	0.0	0.0
27	0.04940539562588286	0.0	0.0	0.0	0.0
28	0.054972200766827406	0.0	0.0	0.0	0.0
29	0.057059752694681616	0.0	0.0	0.0	0.0
30	0.05914730462253582	0.0	0.0	0.0	0.0
31	0.06262655783562616	0.0	0.0	0.0	0.0
32	0.06262655783562616	0.0	0.0	0.0	0.0
33	0.0640182591208623	0.0	0.0	0.0	0.0
34	0.0661058110487165	0.0	0.0	0.0	0.0
35	0.07028091490442491	0.0	0.0	0.0	0.0
36	0.07236846683227911	0.0	0.0	0.0	0.0
37	0.0730643174748972	0.0	0.0	0.0	0.0
38	0.07445601876013333	0.0	0.0	0.0	0.0
39	0.0772394213306056	0.0	0.0	0.0	0.0
40	0.08141452518631401	0.0	0.0	0.0	0.0
41	0.08350207711416821	0.0	0.0	0.0	0.0
42	0.08489377839940435	0.0	0.0	0.0	0.0
43	0.0883730316124947	0.0	0.0	0.0	0.0
44	0.09393983675343924	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGCATTG	30	9.724208E-7	50.498764	39
TAACGTG	15	0.008092048	50.498764	38
CCCCCGA	50	5.456968E-12	50.49876	40
ATATAAT	15	0.008114696	50.46309	37
TATGCTG	20	4.01377E-4	50.39189	35
TCCGGAC	15	0.008160132	50.391888	35
AGATTAC	25	2.0558451E-5	50.091515	49
GATTACG	25	2.0601161E-5	50.07396	50
CATATGT	20	4.1699194E-4	50.003853	33
CCTAGTT	20	4.1915814E-4	49.9514	12
TCCAGCG	15	0.008460098	49.93394	32
CAACTAG	20	4.206072E-4	49.916492	16
ACTAATC	20	4.2133324E-4	49.89906	10
ATGACCC	15	0.008483507	49.89906	10
CGAGAGT	15	0.008495229	49.881634	28
CCGTAGC	20	4.235173E-4	49.84683	27
TCTCTAA	15	0.008518711	49.846825	27
CTCATAG	15	0.008518711	49.846825	19
GCAACGA	15	0.008542241	49.812065	24
GCCCTAC	30	1.0688091E-6	49.812065	26
>>END_MODULE