FastQCFastQC Report
Fri 17 Jun 2016
SRR1526165_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526165_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3588
Sequences flagged as poor quality0
Sequence length40
%GC51

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACGTTGGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTT90.2508361204013378No Hit
ACGTTGGGGGAGTGCAGTGCTACGTTGGGGACACGCCGGG60.16722408026755853No Hit
ACGTTGGGGTGAGTTTTCTATTTTTCTAGTAACAAAGAGT60.16722408026755853No Hit
ACGTTGGGGGCGACCAAGGTGCAGATGGCCTGGGATCGTC50.13935340022296544No Hit
ACGTTGGGGAGAGTCGACTTTTTTTTTTTTTTTTTTTTTT50.13935340022296544No Hit
ACGTTGGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCG40.11148272017837235No Hit
ACGTTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGC40.11148272017837235No Hit
ACGTTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCA40.11148272017837235No Hit
ACGTTGGGGACCTGTGGGTCGTGTGGGAGAGACATGAGTG40.11148272017837235No Hit
ACGTTGGGGGGTTCAGGTCGCAGAGGGCCGTGCTCTCCTC40.11148272017837235No Hit
ACGTTGGGGGCCGAATAGCCTCTCCACCCCAGCGGCCGGA40.11148272017837235No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTGGGGT200.002297008634.3857154
TTGGGGG1150.034.3857154
TGGGGGG404.3932232E-834.3857155
TGGGGGA356.741502E-734.3857155
TGGGGCT200.002297008634.3857155
GGGGGGG200.002297008634.3857156
TGGGGGC251.5401542E-434.385715
CGTTGGG3650.032.5015642
GTTGGGG3350.032.3328363
ACGTTGG3750.032.0933341
TTGGGGC603.092282E-1131.5202374
TGGGGAC507.936251E-930.9471425
TTGGGGA1450.029.6428594
TGGGGAG304.4953157E-428.654765
GGGAAGC250.00685413427.5085727
TGGGGAA653.0177034E-926.4505485
GGGGAAG501.09174525E-524.076
Produced by FastQC (version 0.11.5)