##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1526165_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3588
Sequences flagged as poor quality	0
Sequence length	40
%GC	51
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	24.151895206243033	26.0	21.0	31.0	4.0	33.0
2	13.383221850613156	11.0	11.0	15.0	4.0	25.0
3	21.664715719063544	23.0	16.0	29.0	4.0	33.0
4	23.80128205128205	27.0	20.0	30.0	4.0	33.0
5	23.377647714604237	26.0	19.0	30.0	4.0	33.0
6	22.61315496098105	26.0	16.0	30.0	4.0	34.0
7	20.886008918617613	23.0	12.0	30.0	4.0	33.0
8	21.977146042363433	25.0	13.0	31.0	4.0	34.0
9	20.316610925306577	23.0	10.0	29.0	4.0	33.0
10	19.399386845039018	20.0	10.0	28.0	4.0	33.0
11	22.58026755852843	26.0	12.0	33.0	4.0	37.0
12	22.433946488294314	25.0	12.0	33.0	4.0	37.0
13	23.472965440356745	27.0	13.0	34.0	4.0	37.0
14	23.846153846153847	28.0	13.0	35.0	4.0	38.0
15	22.4484392419175	26.0	10.0	34.0	4.0	37.0
16	20.2603121516165	23.0	4.0	32.0	4.0	36.0
17	20.566610925306577	24.0	4.0	32.0	4.0	36.0
18	20.4350613154961	24.0	4.0	33.0	4.0	36.0
19	18.204570791527313	18.0	4.0	30.0	4.0	35.0
20	19.270624303233	21.0	4.0	32.0	4.0	35.0
21	17.221571906354516	15.0	4.0	30.0	4.0	35.0
22	16.880992196209586	15.0	4.0	30.0	4.0	35.0
23	16.707357859531772	15.0	4.0	30.0	4.0	34.0
24	16.468784838350057	13.0	4.0	30.0	4.0	34.0
25	15.719620958751394	9.0	4.0	30.0	4.0	34.0
26	13.653288740245262	4.0	4.0	26.0	4.0	33.0
27	13.366499442586399	4.0	4.0	26.0	4.0	33.0
28	13.10340022296544	4.0	4.0	25.0	4.0	33.0
29	12.7773132664437	4.0	4.0	25.0	4.0	33.0
30	12.024247491638796	4.0	4.0	23.0	4.0	32.0
31	11.61510590858417	4.0	4.0	23.0	4.0	32.0
32	11.118729096989966	4.0	4.0	19.0	4.0	31.0
33	10.402173913043478	4.0	4.0	16.0	4.0	31.0
34	9.942865105908584	4.0	4.0	13.0	4.0	30.0
35	9.284559643255296	4.0	4.0	9.0	4.0	29.0
36	9.091415830546266	4.0	4.0	4.0	4.0	30.0
37	9.107859531772576	4.0	4.0	4.0	4.0	31.0
38	8.753344481605351	4.0	4.0	4.0	4.0	30.0
39	8.418338907469343	4.0	4.0	4.0	4.0	28.0
40	8.42670011148272	4.0	4.0	4.0	4.0	28.0
>>END_MODULE
>>Per tile sequence quality	fail
#Tile	Base	Mean
1	1	0.040710034013606844
1	2	12.898745748299325
1	3	-1.3903698979591823
1	4	2.0838647959183554
1	5	2.3194940476190418
1	6	2.7514030612244937
1	7	4.663392857142856
1	8	-1.6620642006802662
1	9	-3.815561224489791
1	10	-0.021481717687073854
1	11	-4.582621173469388
1	12	-2.722629676870749
1	13	-7.86776147959184
1	14	-4.385140306122441
1	15	-4.123511904761912
1	16	-7.662159863945577
1	17	-5.392463860544215
1	18	-7.223001700680273
1	19	-6.132504251700679
1	20	-4.697725340136055
1	21	-3.3192283163265284
1	22	-4.22997448979592
1	23	-2.364221938775513
1	24	-1.2745429421768701
1	25	-1.5041666666666664
1	26	-2.502104591836737
1	27	0.786203231292518
1	28	-1.6058673469387728
1	29	-5.808630952380954
1	30	-2.5580888605442205
1	31	-2.216879251700684
1	32	-1.0957057823129244
1	33	-3.3668473639455767
1	34	-2.553348214285715
1	35	-2.4244366496598646
1	36	-1.402699829931974
1	37	1.3931122448979574
1	38	-1.398320578231293
1	39	-1.356207482993197
1	40	-0.9534651360544233
3	1	-3.7926232993197253
3	2	5.732079081632657
3	3	11.776296768707486
3	4	9.583864795918355
3	5	10.98616071428571
3	6	8.918069727891162
3	7	-5.503273809523812
3	8	-8.328730867346934
3	9	-6.315561224489791
3	10	6.811851615646258
3	11	4.917378826530612
3	12	7.277370323129251
3	13	11.465571853741494
3	14	11.948193027210891
3	15	11.043154761904756
3	16	-5.828826530612243
3	17	11.107536139455785
3	18	14.443664965986393
3	19	2.200829081632655
3	20	2.6356079931972793
3	21	12.347438350340138
3	22	1.2700255102040803
3	23	12.635778061224487
3	24	15.72545705782313
3	25	14.995833333333334
3	26	20.331228741496595
3	27	21.952869897959182
3	28	17.56079931972789
3	29	22.191369047619048
3	30	16.108577806122447
3	31	15.283120748299316
3	32	20.73762755102041
3	33	3.4664859693877563
3	34	22.113318452380952
3	35	5.242230017006802
3	36	5.597300170068026
3	37	23.226445578231292
3	38	5.601679421768707
3	39	23.6437925170068
3	40	-2.9534651360544233
5	1	-9.459289965986391
5	2	-6.93458758503401
5	3	-11.39036989795918
5	4	-14.08280187074831
5	5	-15.347172619047623
5	6	-12.248596938775504
5	7	-12.336607142857144
5	8	-11.9953975340136
5	9	-10.982227891156457
5	10	-9.354815051020408
5	11	-11.749287840136054
5	12	-12.055963010204083
5	13	-13.36776147959184
5	14	-13.718473639455775
5	15	-12.790178571428578
5	16	-9.99549319727891
5	17	-10.392463860544215
5	18	-11.889668367346939
5	19	-11.465837585034013
5	20	-12.197725340136053
5	21	-8.985894982993194
5	22	-8.896641156462586
5	23	-6.864221938775513
5	24	-11.27454294217687
5	25	-10.004166666666666
5	26	-8.668771258503403
5	27	-8.047130102040816
5	28	-8.439200680272107
5	29	-7.808630952380954
5	30	-6.891422193877554
5	31	-6.716879251700684
5	32	-6.2623724489795904
5	33	-5.533514030612244
5	34	-4.886681547619048
5	35	-3.7577699829931976
5	36	-3.402699829931974
5	37	-3.7735544217687087
5	38	-3.398320578231293
5	39	-3.356207482993197
5	40	-2.9534651360544233
6	1	4.540710034013607
6	2	5.065412414965989
6	3	-3.5570365646258466
6	4	-5.0828018707483125
6	5	0.31949404761904177
6	6	5.584736394557829
6	7	9.496726190476188
6	8	3.671269132653066
6	9	6.351105442176877
6	10	6.1451849489795904
6	11	11.250712159863948
6	12	11.610703656462587
6	13	11.132238520408158
6	14	11.614859693877555
6	15	8.376488095238088
6	16	3.1711734693877567
6	17	13.107536139455785
6	18	9.443664965986393
6	19	4.200829081632655
6	20	13.302274659863947
6	21	0.3474383503401377
6	22	7.936692176870748
6	23	-0.6975552721088469
6	24	2.72545705782313
6	25	7.662500000000001
6	26	3.9978954081632647
6	27	5.952869897959184
6	28	8.227465986394561
6	29	4.858035714285714
6	30	5.441911139455778
6	31	5.616454081632648
6	32	10.070960884353742
6	33	12.133152636054424
6	34	4.113318452380952
6	35	3.5755633503401363
6	36	5.93063350340136
6	37	5.559778911564625
6	38	5.935012755102041
6	39	5.977125850340137
6	40	6.379868197278911
7	1	6.957376700680275
7	2	2.482079081632657
7	3	8.776296768707486
7	4	10.333864795918355
7	5	4.98616071428571
7	6	2.6680697278911616
7	7	5.246726190476188
7	8	-3.078730867346934
7	9	7.684438775510209
7	10	8.311851615646258
7	11	11.417378826530612
7	12	7.277370323129251
7	13	14.215571853741494
7	14	12.198193027210891
7	15	13.793154761904756
7	16	6.671173469387757
7	17	7.107536139455785
7	18	0.6936649659863932
7	19	-3.549170918367345
7	20	0.8856079931972793
7	21	-4.152561649659862
7	22	-4.72997448979592
7	23	-2.864221938775513
7	24	-3.27454294217687
7	25	-1.7541666666666664
7	26	-8.668771258503403
7	27	-8.047130102040816
7	28	-8.439200680272107
7	29	-7.808630952380954
7	30	-6.891422193877554
7	31	-6.716879251700684
7	32	-6.2623724489795904
7	33	-5.533514030612244
7	34	-4.886681547619048
7	35	-3.7577699829931976
7	36	-3.402699829931974
7	37	-3.7735544217687087
7	38	-3.398320578231293
7	39	-3.356207482993197
7	40	-2.9534651360544233
8	1	-8.792623299319725
8	2	-1.6679209183673436
8	3	-4.623703231292515
8	4	-5.216135204081645
8	5	-3.813839285714291
8	6	-7.081930272108838
8	7	-9.703273809523811
8	8	-12.328730867346934
8	9	-10.115561224489792
8	10	-8.988148384353742
8	11	1.1173788265306115
8	12	-9.52262967687075
8	13	-6.934428146258506
8	14	-1.8518069727891096
8	15	-12.756845238095245
8	16	-8.428826530612243
8	17	-12.492463860544214
8	18	-4.1563350340136065
8	19	-2.9991709183673443
8	20	-4.364392006802721
8	21	-3.8525616496598616
8	22	-4.52997448979592
8	23	-1.364221938775513
8	24	-3.4745429421768694
8	25	0.3958333333333339
8	26	2.1312287414965976
8	27	-3.8471301020408166
8	28	-3.0392006802721063
8	29	-2.4086309523809533
8	30	-6.891422193877554
8	31	-6.716879251700684
8	32	-6.2623724489795904
8	33	-5.533514030612244
8	34	-4.886681547619048
8	35	-3.7577699829931976
8	36	-3.402699829931974
8	37	-3.7735544217687087
8	38	-3.398320578231293
8	39	-3.356207482993197
8	40	-2.9534651360544233
9	1	4.040710034013607
9	2	11.732079081632657
9	3	4.109630102040818
9	4	12.75053146258502
9	5	11.819494047619045
9	6	10.751403061224494
9	7	11.163392857142856
9	8	7.504602465986398
9	9	10.684438775510209
9	10	8.478518282312926
9	11	5.750712159863944
9	12	12.444036989795915
9	13	9.632238520408162
9	14	4.781526360544223
9	15	1.0431547619047556
9	16	6.3378401360544245
9	17	7.607536139455785
9	18	10.443664965986393
9	19	6.367495748299323
9	20	6.635607993197279
9	21	2.8474383503401377
9	22	4.27002551020408
9	23	5.969111394557819
9	24	4.558790391156462
9	25	5.162500000000001
9	26	1.8312287414965969
9	27	1.286203231292518
9	28	-1.1058673469387728
9	29	-0.9752976190476197
9	30	-0.5580888605442205
9	31	-0.88354591836735
9	32	0.5709608843537435
9	33	-2.3668473639455767
9	34	0.9466517857142858
9	35	2.0755633503401363
9	36	4.763966836734692
9	37	7.059778911564625
9	38	2.935012755102041
9	39	1.8104591836734691
9	40	4.546534863945577
10	1	3.9573767006802747
10	2	0.7320790816326568
10	3	8.026296768707486
10	4	6.958864795918355
10	5	2.2361607142857096
10	6	6.918069727891162
10	7	7.496726190476188
10	8	8.546269132653066
10	9	7.559438775510209
10	10	7.061851615646258
10	11	9.917378826530612
10	12	11.90237032312925
10	13	13.090571853741494
10	14	10.573193027210891
10	15	10.043154761904756
10	16	7.171173469387757
10	17	9.857536139455785
10	18	8.568664965986393
10	19	12.075829081632655
10	20	5.510607993197279
10	21	3.3474383503401377
10	22	8.39502551020408
10	23	6.135778061224487
10	24	2.10045705782313
10	25	4.995833333333334
10	26	-4.043771258503403
10	27	-0.4221301020408159
10	28	-4.564200680272107
10	29	-3.5586309523809536
10	30	-6.016422193877554
10	31	-3.466879251700684
10	32	-3.3873724489795904
10	33	-3.9085140306122437
10	34	-2.761681547619048
10	35	-0.8827699829931976
10	36	-0.15269982993197395
10	37	-3.7735544217687087
10	38	-3.398320578231293
10	39	-3.356207482993197
10	40	-2.9534651360544233
11	1	6.457376700680275
11	2	10.732079081632657
11	3	12.276296768707486
11	4	6.458864795918355
11	5	10.73616071428571
11	6	12.418069727891162
11	7	9.746726190476188
11	8	12.171269132653066
11	9	11.559438775510209
11	10	11.186851615646258
11	11	11.792378826530612
11	12	10.52737032312925
11	13	12.215571853741494
11	14	12.323193027210891
11	15	10.543154761904756
11	16	5.921173469387757
11	17	14.732536139455785
11	18	14.068664965986393
11	19	9.325829081632655
11	20	11.01060799319728
11	21	9.722438350340138
11	22	9.14502551020408
11	23	10.885778061224487
11	24	13.97545705782313
11	25	14.995833333333334
11	26	14.456228741496597
11	27	8.827869897959184
11	28	13.435799319727893
11	29	10.191369047619046
11	30	10.108577806122446
11	31	12.033120748299316
11	32	14.36262755102041
11	33	8.966485969387756
11	34	8.363318452380952
11	35	7.242230017006802
11	36	2.097300170068026
11	37	3.7264455782312913
11	38	2.601679421768707
11	39	2.893792517006803
11	40	1.5465348639455767
12	1	-0.19262329931972388
12	2	3.132079081632657
12	3	1.9762967687074848
12	4	2.983864795918354
12	5	1.586160714285711
12	6	-2.0819302721088384
12	7	-4.503273809523812
12	8	0.8712691326530653
12	9	-5.915561224489791
12	10	1.8118516156462583
12	11	6.317378826530611
12	12	7.277370323129251
12	13	6.265571853741495
12	14	-1.2518069727891081
12	15	5.443154761904754
12	16	5.371173469387756
12	17	4.107536139455785
12	18	11.643664965986392
12	19	9.600829081632654
12	20	9.035607993197278
12	21	5.547438350340137
12	22	7.870025510204082
12	23	11.635778061224487
12	24	11.92545705782313
12	25	7.195833333333333
12	26	1.3312287414965969
12	27	3.3528698979591844
12	28	7.960799319727892
12	29	2.791369047619046
12	30	-1.2914221938775547
12	31	-0.9168792517006832
12	32	-2.4623724489795906
12	33	-1.5335140306122437
12	34	0.31331845238095113
12	35	-3.7577699829931976
12	36	-3.402699829931974
12	37	-3.7735544217687087
12	38	-3.398320578231293
12	39	-3.356207482993197
12	40	-2.9534651360544233
13	1	3.3740433673469425
13	2	0.5654124149659907
13	3	3.4429634353741534
13	4	3.4171981292516875
13	5	4.819494047619042
13	6	4.084736394557829
13	7	2.9967261904761884
13	8	4.004602465986398
13	9	4.184438775510209
13	10	4.478518282312926
13	11	13.250712159863948
13	12	10.944036989795919
13	13	8.632238520408162
13	14	11.614859693877555
13	15	11.043154761904756
13	16	14.337840136054421
13	17	9.774202806122453
13	18	11.110331632653061
13	19	11.200829081632655
13	20	4.135607993197279
13	21	6.0141050170068056
13	22	6.103358843537412
13	23	9.302444727891155
13	24	6.558790391156462
13	25	9.162500000000001
13	26	3.664562074829929
13	27	4.619536564625852
13	28	2.5607993197278933
13	29	11.024702380952379
13	30	7.441911139455778
13	31	6.949787414965984
13	32	-4.429039115646257
13	33	3.4664859693877563
13	34	-1.2200148809523812
13	35	-3.0911033163265307
13	36	-2.736033163265307
13	37	1.0597789115646252
13	38	1.4350127551020408
13	39	0.6437925170068031
13	40	1.8798681972789106
14	1	13.207376700680275
14	2	10.732079081632657
14	3	15.776296768707486
14	4	1.5838647959183554
14	5	13.98616071428571
14	6	16.91806972789116
14	7	18.49672619047619
14	8	17.671269132653066
14	9	18.68443877551021
14	10	19.81185161564626
14	11	10.917378826530612
14	12	11.27737032312925
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110	38	-3.398320578231293
110	39	-3.356207482993197
110	40	-2.9534651360544233
111	1	9.957376700680275
111	2	5.982079081632657
111	3	7.7762967687074855
111	4	5.083864795918355
111	5	0.9861607142857096
111	6	4.168069727891162
111	7	1.4967261904761884
111	8	5.421269132653066
111	9	0.18443877551020904
111	10	6.061851615646258
111	11	0.9173788265306122
111	12	6.527370323129251
111	13	2.215571853741494
111	14	7.698193027210891
111	15	3.2931547619047556
111	16	4.921173469387757
111	17	4.857536139455785
111	18	4.193664965986393
111	19	10.950829081632655
111	20	3.6356079931972793
111	21	11.347438350340138
111	22	13.02002551020408
111	23	3.135778061224487
111	24	13.97545705782313
111	25	5.995833333333334
111	26	7.081228741496597
111	27	7.702869897959184
111	28	7.310799319727893
111	29	3.9413690476190464
111	30	6.358577806122446
111	31	9.533120748299316
111	32	8.73762755102041
111	33	10.716485969387756
111	34	8.613318452380952
111	35	11.742230017006802
111	36	-3.402699829931974
111	37	-3.7735544217687087
111	38	-3.398320578231293
111	39	-3.356207482993197
111	40	-2.9534651360544233
112	1	11.207376700680275
112	2	12.232079081632657
112	3	6.7762967687074855
112	4	10.083864795918355
112	5	5.48616071428571
112	6	-0.08193027210883841
112	7	10.496726190476188
112	8	-10.328730867346934
112	9	-3.315561224489791
112	10	-11.188148384353742
112	11	-13.582621173469388
112	12	-14.72262967687075
112	13	-12.034428146258506
112	14	-10.551806972789109
112	15	-12.456845238095244
112	16	-11.328826530612243
112	17	-4.892463860544215
112	18	-1.5563350340136068
112	19	-7.799170918367345
112	20	-13.86439200680272
112	21	-5.152561649659862
112	22	-8.72997448979592
112	23	-8.364221938775513
112	24	-8.27454294217687
112	25	-8.504166666666666
112	26	-2.668771258503403
112	27	-4.047130102040816
112	28	-7.439200680272107
112	29	-6.308630952380954
112	30	-0.8914221938775544
112	31	8.283120748299316
112	32	2.2376275510204096
112	33	7.466485969387756
112	34	7.613318452380952
112	35	4.742230017006802
112	36	12.097300170068026
112	37	11.726445578231292
112	38	12.101679421768708
112	39	12.143792517006803
112	40	12.546534863945578
113	1	8.957376700680275
113	2	3.982079081632657
113	3	7.2762967687074855
113	4	4.583864795918355
113	5	6.73616071428571
113	6	10.918069727891162
113	7	7.746726190476188
113	8	5.421269132653066
113	9	6.934438775510209
113	10	6.811851615646258
113	11	-1.8326211734693878
113	12	7.527370323129251
113	13	2.465571853741494
113	14	4.198193027210891
113	15	5.043154761904756
113	16	9.171173469387757
113	17	10.107536139455785
113	18	11.943664965986393
113	19	11.700829081632655
113	20	13.13560799319728
113	21	12.347438350340138
113	22	10.52002551020408
113	23	7.885778061224487
113	24	11.47545705782313
113	25	16.245833333333334
113	26	3.331228741496597
113	27	2.702869897959184
113	28	2.8107993197278933
113	29	2.6913690476190464
113	30	1.1085778061224456
113	31	-5.966879251700684
113	32	0.9876275510204096
113	33	1.7164859693877563
113	34	2.363318452380952
113	35	3.4922300170068024
113	36	3.347300170068026
113	37	-3.7735544217687087
113	38	-3.398320578231293
113	39	-3.356207482993197
113	40	-2.9534651360544233
116	1	-9.792623299319725
116	2	-4.767920918367343
116	3	-7.7237032312925145
116	4	-10.916135204081645
116	5	-6.51383928571429
116	6	-3.5819302721088384
116	7	-2.5032738095238116
116	8	-3.828730867346934
116	9	-1.815561224489791
116	10	-6.188148384353742
116	11	-5.582621173469388
116	12	-3.722629676870749
116	13	-16.534428146258506
116	14	-6.551806972789109
116	15	-4.956845238095244
116	16	-14.828826530612243
116	17	-14.892463860544215
116	18	-14.556335034013607
116	19	-12.799170918367345
116	20	-14.36439200680272
116	21	-12.652561649659862
116	22	-11.72997448979592
116	23	-11.364221938775513
116	24	-11.27454294217687
116	25	-10.004166666666666
116	26	-8.668771258503403
116	27	-8.047130102040816
116	28	-8.439200680272107
116	29	-7.808630952380954
116	30	-6.891422193877554
116	31	-6.716879251700684
116	32	-6.2623724489795904
116	33	-5.533514030612244
116	34	-4.886681547619048
116	35	-3.7577699829931976
116	36	-3.402699829931974
116	37	-3.7735544217687087
116	38	-3.398320578231293
116	39	-3.356207482993197
116	40	-2.9534651360544233
119	1	2.2073767006802747
119	2	4.732079081632657
119	3	0.7762967687074855
119	4	12.583864795918355
119	5	12.98616071428571
119	6	14.918069727891162
119	7	10.496726190476188
119	8	9.671269132653066
119	9	13.684438775510209
119	10	14.811851615646258
119	11	11.917378826530612
119	12	-0.7226296768707492
119	13	-10.534428146258506
119	14	6.948193027210891
119	15	14.043154761904756
119	16	14.171173469387757
119	17	-7.892463860544215
119	18	6.443664965986393
119	19	2.200829081632655
119	20	14.63560799319728
119	21	8.347438350340138
119	22	20.27002551020408
119	23	9.635778061224487
119	24	17.725457057823128
119	25	-4.004166666666666
119	26	22.331228741496595
119	27	17.952869897959182
119	28	17.56079931972789
119	29	18.191369047619048
119	30	-6.891422193877554
119	31	-6.716879251700684
119	32	-6.2623724489795904
119	33	-5.533514030612244
119	34	-4.886681547619048
119	35	-3.7577699829931976
119	36	-3.402699829931974
119	37	-3.7735544217687087
119	38	-3.398320578231293
119	39	-3.356207482993197
119	40	-2.9534651360544233
>>END_MODULE
>>Per sequence quality scores	fail
#Quality	Count
4	496.0
5	32.0
6	63.0
7	42.0
8	37.0
9	53.0
10	98.0
11	141.0
12	139.0
13	141.0
14	134.0
15	144.0
16	165.0
17	180.0
18	195.0
19	181.0
20	166.0
21	174.0
22	166.0
23	159.0
24	162.0
25	150.0
26	114.0
27	97.0
28	58.0
29	41.0
30	29.0
31	18.0
32	6.0
33	5.0
34	1.0
35	1.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.0	100.0	0.0	0.0
2	0.0	0.0	0.0	100.0
3	100.0	0.0	0.0	0.0
4	0.0	0.0	100.0	0.0
5	0.0	0.0	100.0	0.0
6	100.0	0.0	0.0	0.0
7	99.47030945079453	0.3902982994145526	0.027878449958182325	0.1115137998327293
8	97.90736607142857	0.78125	0.5301339285714286	0.78125
9	93.19765821020351	3.7357122943964316	1.4775578477836633	1.5890716476163926
10	35.089186176142704	40.078037904124855	10.117056856187292	14.715719063545151
11	28.901895206243033	31.24303232998885	20.930880713489408	18.92419175027871
12	36.20401337792642	17.920847268673356	22.212931995540693	23.662207357859533
13	26.226309921962095	21.460423634336678	24.16387959866221	28.14938684503902
14	23.662207357859533	27.369007803790414	28.678929765886284	20.28985507246377
15	26.282051282051285	24.442586399108137	26.477146042363437	22.798216276477145
16	28.093645484949832	23.717948717948715	27.452619843924193	20.735785953177256
17	29.375696767001113	23.940914158305464	24.24749163879599	22.435897435897438
18	29.013377926421402	23.91304347826087	22.547380156075807	24.526198439241917
19	21.326616288832913	16.288832913518053	20.207108872096278	42.177441925552756
20	23.10479375696767	27.898550724637683	23.634336677814936	25.36231884057971
21	22.85395763656633	20.958751393534	29.208472686733554	26.978818283166113
22	24.24749163879599	19.007803790412485	22.9933110367893	33.751393534002226
23	25.27870680044593	24.414715719063544	26.30992196209587	23.99665551839465
24	22.74247491638796	23.662207357859533	26.031215161649946	27.564102564102566
25	24.239039374476405	25.439821278972353	25.439821278972353	24.88131806757889
26	19.971988795518207	24.201680672268907	30.30812324929972	25.518207282913163
27	23.9542665923034	20.30117122141662	28.30451756832125	27.44004461795873
28	20.71946458449526	24.093697713329615	28.611266034578918	26.575571667596208
29	18.9453125	21.372767857142858	31.38950892857143	28.292410714285715
30	17.038482989403235	20.245398773006134	34.71834913552705	27.99776910206358
31	16.829745596868882	23.427453173050043	38.91529214425496	20.82750908582611
32	18.901589071647617	18.148870922776695	38.08196264287705	24.867577362698633
33	16.30889322553666	20.574296069138555	38.69528854195707	24.42152216336772
34	13.600891861761427	19.899665551839465	43.47826086956522	23.02118171683389
35	14.357401728463898	20.100362419849457	42.040702536938944	23.5015333147477
36	14.552550878171175	19.737942570393084	39.75466964036799	25.954836911067748
37	13.699776785714285	20.870535714285715	42.103794642857146	23.325892857142858
38	14.381270903010032	21.153846153846153	42.7536231884058	21.711259754738016
39	15.751324226373015	22.470030666294953	39.97769724003346	21.80094786729858
40	13.879598662207357	22.547380156075807	41.02564102564102	22.547380156075807
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	2.5
15	5.0
16	9.5
17	14.0
18	14.0
19	19.5
20	25.0
21	27.0
22	29.0
23	29.0
24	37.5
25	46.0
26	57.0
27	68.0
28	68.0
29	63.0
30	58.0
31	69.0
32	80.0
33	80.0
34	113.5
35	147.0
36	154.5
37	162.0
38	162.0
39	187.0
40	212.0
41	212.0
42	212.0
43	212.0
44	225.5
45	239.0
46	251.0
47	263.0
48	263.0
49	247.0
50	231.0
51	223.5
52	216.0
53	216.0
54	221.5
55	227.0
56	227.5
57	228.0
58	228.0
59	232.5
60	237.0
61	240.0
62	243.0
63	243.0
64	232.5
65	222.0
66	193.5
67	165.0
68	165.0
69	140.0
70	115.0
71	98.0
72	81.0
73	81.0
74	57.5
75	34.0
76	27.0
77	20.0
78	20.0
79	11.0
80	2.0
81	4.5
82	7.0
83	7.0
84	3.5
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.027870680044593088
8	0.11148272017837235
9	0.027870680044593088
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.4180602006688963
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.19509476031215162
26	0.5016722408026756
27	0.055741360089186176
28	0.055741360089186176
29	0.11148272017837235
30	0.055741360089186176
31	0.30657748049052397
32	0.027870680044593088
33	0.027870680044593088
34	0.0
35	0.027870680044593088
36	0.027870680044593088
37	0.11148272017837235
38	0.0
39	0.027870680044593088
40	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
40	3588.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	95.37346711259754
#Duplication Level	Percentage of deduplicated	Percentage of total
1	96.69783752191701	92.22408026755853
2	2.396259497369959	4.570791527313267
3	0.5844535359438925	1.6722408026755853
4	0.17533606078316774	0.6688963210702341
5	0.058445353594389245	0.2787068004459309
6	0.058445353594389245	0.33444816053511706
7	0.0	0.0
8	0.0	0.0
9	0.029222676797194622	0.2508361204013378
>10	0.0	0.0
>50	0.0	0.0
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
ACGTTGGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTT	9	0.2508361204013378	No Hit
ACGTTGGGGGAGTGCAGTGCTACGTTGGGGACACGCCGGG	6	0.16722408026755853	No Hit
ACGTTGGGGTGAGTTTTCTATTTTTCTAGTAACAAAGAGT	6	0.16722408026755853	No Hit
ACGTTGGGGGCGACCAAGGTGCAGATGGCCTGGGATCGTC	5	0.13935340022296544	No Hit
ACGTTGGGGAGAGTCGACTTTTTTTTTTTTTTTTTTTTTT	5	0.13935340022296544	No Hit
ACGTTGGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCG	4	0.11148272017837235	No Hit
ACGTTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGC	4	0.11148272017837235	No Hit
ACGTTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCA	4	0.11148272017837235	No Hit
ACGTTGGGGACCTGTGGGTCGTGTGGGAGAGACATGAGTG	4	0.11148272017837235	No Hit
ACGTTGGGGGGTTCAGGTCGCAGAGGGCCGTGCTCTCCTC	4	0.11148272017837235	No Hit
ACGTTGGGGGCCGAATAGCCTCTCCACCCCAGCGGCCGGA	4	0.11148272017837235	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.027870680044593088	0.0	0.0	0.0	0.0
10	0.055741360089186176	0.0	0.0	0.0	0.0
11	0.08361204013377926	0.0	0.0	0.0	0.0
12	0.08361204013377926	0.0	0.0	0.0	0.0
13	0.08361204013377926	0.0	0.0	0.0	0.0
14	0.08361204013377926	0.0	0.0	0.0	0.0
15	0.08361204013377926	0.0	0.0	0.0	0.0
16	0.08361204013377926	0.0	0.0	0.0	0.0
17	0.08361204013377926	0.0	0.0	0.0	0.0
18	0.08361204013377926	0.0	0.0	0.0	0.0
19	0.08361204013377926	0.0	0.0	0.0	0.0
20	0.08361204013377926	0.0	0.0	0.0	0.0
21	0.08361204013377926	0.0	0.0	0.0	0.0
22	0.08361204013377926	0.0	0.0	0.0	0.0
23	0.08361204013377926	0.0	0.0	0.0	0.0
24	0.08361204013377926	0.0	0.0	0.0	0.0
25	0.08361204013377926	0.0	0.0	0.0	0.0
26	0.08361204013377926	0.0	0.0	0.0	0.0
27	0.08361204013377926	0.0	0.0	0.0	0.0
28	0.08361204013377926	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTGGGGT	20	0.0022970086	34.385715	4
TTGGGGG	115	0.0	34.385715	4
TGGGGGG	40	4.3932232E-8	34.385715	5
TGGGGGA	35	6.741502E-7	34.385715	5
TGGGGCT	20	0.0022970086	34.385715	5
GGGGGGG	20	0.0022970086	34.385715	6
TGGGGGC	25	1.5401542E-4	34.38571	5
CGTTGGG	365	0.0	32.501564	2
GTTGGGG	335	0.0	32.332836	3
ACGTTGG	375	0.0	32.093334	1
TTGGGGC	60	3.092282E-11	31.520237	4
TGGGGAC	50	7.936251E-9	30.947142	5
TTGGGGA	145	0.0	29.642859	4
TGGGGAG	30	4.4953157E-4	28.65476	5
GGGAAGC	25	0.006854134	27.508572	7
TGGGGAA	65	3.0177034E-9	26.450548	5
GGGGAAG	50	1.09174525E-5	24.07	6
>>END_MODULE