FastQCFastQC Report
Fri 17 Jun 2016
SRR1526163_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526163_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10782
Sequences flagged as poor quality0
Sequence length40
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACGTTGGGGTACCTGGTTGATCCTGCCAGTAGCATATGCT5655.2402151734372096No Hit
ACGTTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGC1801.669449081803005No Hit
ACGTTGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA910.8439992580226303No Hit
ACGTTGGGGTCACAGATCCTCTTCTGAAGGAGATTGGGCG450.41736227045075125No Hit
ACGTTGGGGTCACAGATCCTCTTCTGAGATGAGTTTTTGT440.4080875533296235No Hit
ACGTTGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAG420.38953811908736785No Hit
ACGTTGGGGTCCCTGGTTGATCCTGCCAGTAGCATATGCT330.3060656649972176No Hit
ACGTTGGGGTACCTGGTTGATACTGCCAGTAGCATATGCT240.22259321090706735No Hit
ACGTTGGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGC230.21331849378593953No Hit
ACGTTGGGGTAACTGGTTGATCCTGCCAGTAGCATATGCT210.19476905954368393No Hit
ACGTTGGGGTACCTGGTTGATCCTGCCAGTAGCATATGCC200.1854943424225561No Hit
ACGTTGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA200.1854943424225561No Hit
ACGTTGGGGATGCCACAACTAGATACATCAACATGATTTA170.1576701910591727No Hit
ACGTTGGGGACTAATCCTAGCCCTAGCCCTACACAAATAT170.1576701910591727No Hit
ACGTTGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCAC150.13912075681691707No Hit
ACGTTGGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGA140.12984603969578928No Hit
ACGTTGGGGTCAAAGATCCTCTTCTGAGATGAGTTTTTGT140.12984603969578928No Hit
ACGTTGGGGTAAATGGTTGATCCTGCCAGTAGCATATGCT130.12057132257466147No Hit
ACGTTGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTT130.12057132257466147No Hit
ACGTTGGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCG130.12057132257466147No Hit
ACGTTGGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAA130.12057132257466147No Hit
ACGTTGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA130.12057132257466147No Hit
ACGTTGGGGTACATGGTTGATCCTGCCAGTAGCATATGCT130.12057132257466147No Hit
ACGTTGGGGGTTTTTGGGAGTCTCATCATTGGCTATGCCA120.11129660545353368No Hit
ACGTTGGGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAG120.11129660545353368No Hit
ACGTTGGGGTCAAAGATCCTCTTCTGAAGGAGATTGGGCG120.11129660545353368No Hit
ACGTTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCA110.10202188833240587No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGAGTTT301.29845375E-534.19718630
ATCCTCT251.8852449E-434.03738416
TGGGGTC552.3646862E-1134.0373845
TGGGGTA1100.034.0373845
TTGGGGT1800.034.0373844
TGGGGGC650.034.0373845
TGGGGAG552.3646862E-1134.0373845
TGGGGAC251.8852449E-434.0373845
GATCCTC251.8852449E-434.03738415
ATATGCT900.034.03738434
CAGATCC301.34139755E-534.03738413
AGATCCT301.34139755E-534.03738414
ATGAGTT251.8852449E-434.03738429
GGGTCAC406.805567E-834.037387
GTCACAG359.553532E-734.037389
GGTCACA406.805567E-834.037388
TGGGGCC359.553532E-734.037385
GGGGCTT200.002653566434.037387
CTGAGAT251.9382285E-433.8790724
ACAGATC301.3855439E-533.8790712
Produced by FastQC (version 0.11.5)