FastQCFastQC Report
Fri 17 Jun 2016
SRR1526162_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526162_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences179188
Sequences flagged as poor quality0
Sequence length56
%GC54

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTACCGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAG31801.7746724110989573No Hit
TCTACCGGGTCACAGATCCTCTTCTGAGATGAGTTTTTGTTCAATCCGCCCAATCT10920.609415809094359No Hit
TCTACCGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTCGTCTCAAAGATTAAG9060.5056142152376275No Hit
TCTACCGGGTCACAGATCCTCTTCTGAAGGAGATTGGGCGGATTGAACAAAAACTC8870.49501082661785384No Hit
TCTACCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC5290.29522066209790837No Hit
TCTACCGGGTACCTGGTTGATCCTGCCAGTAGCATATGCCTGTCTCAAAGATTAAG3940.21988079558899035No Hit
TCTACCGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTC3910.2182065763332366No Hit
TCTACCGGGTCACAGATCCTCTTCTGAAGGAGATTGGGCGCATTGAACAAAAACTC3700.20648704154296046No Hit
TCTACCGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCCAAGATTAAG3450.19253521441167937No Hit
TCTACCGGGTCACAGATCCTCTTCTGAGATGAGTTTTTGTCCAATCCGCCCAATCT2920.1629573408933634No Hit
TCTACCGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCCACGATTAAG2670.1490055137620823No Hit
TCTACCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCCCCCGACTGCTCTTCC2280.12724066343728374No Hit
TCTACCGGGCTCCTACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCGAC2240.12500837109627877No Hit
TCTACCGGGGGGCGCTGACCCCCCTTCCCGGGGGGGGATGCGTGCATTTATCAGAT2000.1116146170502489No Hit
TCTACCGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGC1860.10380159385673147No Hit
TCTACCGGTCACAGATCCTCTTCTGAGATGAGTTTTTGTTCAATCCGCCCAATCTC1830.10212737460097775No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCAAT150.00805958350.55533638
AGGGCAC150.00814128350.4265537
ATGCTCG1200.050.28422536
ACAATTA402.562956E-950.2133643
GCCACTA150.00827881750.2133646
GATTCGT204.1561783E-450.04419
CGATTAA507.2759576E-1250.03005249
ACGATTA507.2759576E-1250.01600348
GATTACG252.0936859E-549.94589650
CAACTAG550.049.8759816
GCAATAG150.00853072549.83412633
CGCGATC150.00856852349.7784325
CGGGTCG252.149884E-549.722866
ACCGGTA252.149884E-549.722864
CTACCGA950.049.722862
TACCGCG150.00860644749.722863
CGGGGTA1300.049.7228556
CGGGGTC4350.049.151336
ACCGGGA45200.048.9528164
TACCGGG174000.048.8941463
Produced by FastQC (version 0.11.5)