##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1526162_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	179188
Sequences flagged as poor quality	0
Sequence length	56
%GC	54
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	36.95409848873808	39.0	38.0	39.0	33.0	40.0
2	36.35803736857379	39.0	37.0	39.0	30.0	40.0
3	35.73322432305735	39.0	36.0	39.0	27.0	40.0
4	33.12146460700493	38.0	33.0	39.0	13.0	40.0
5	36.33689756010447	39.0	37.0	39.0	30.0	40.0
6	36.23703038149876	39.0	37.0	39.0	29.0	40.0
7	36.06204656561823	38.0	36.0	39.0	30.0	40.0
8	36.12730763220751	38.0	36.0	39.0	30.0	40.0
9	36.04823983748912	38.0	36.0	39.0	29.0	40.0
10	35.14595285398576	38.0	35.0	39.0	25.0	40.0
11	35.11311025291872	38.0	35.0	39.0	28.0	40.0
12	34.006429001942095	38.0	33.0	39.0	23.0	40.0
13	33.37003593990669	38.0	32.0	39.0	20.0	40.0
14	33.97460767462107	38.0	33.0	39.0	23.0	40.0
15	32.856982610442664	37.0	31.0	39.0	18.0	40.0
16	32.33102663124763	36.0	30.0	38.0	19.0	40.0
17	31.426825457061856	35.0	28.0	38.0	15.0	40.0
18	30.780995379154856	35.0	26.0	38.0	13.0	40.0
19	31.851630689555105	36.0	29.0	38.0	18.0	40.0
20	32.70792687010291	36.0	30.0	38.0	21.0	40.0
21	33.01367279058866	38.0	31.0	39.0	19.0	40.0
22	32.364187333973256	37.0	30.0	39.0	17.0	40.0
23	33.03631939638815	38.0	31.0	39.0	19.0	40.0
24	32.37800522356408	37.0	30.0	39.0	17.0	40.0
25	32.84224948099203	37.0	31.0	39.0	19.0	40.0
26	33.40295109047481	38.0	33.0	39.0	20.0	40.0
27	33.631867089314014	38.0	33.0	39.0	22.0	40.0
28	33.54015893921468	38.0	33.0	39.0	23.0	40.0
29	33.10571020380829	38.0	32.0	39.0	19.0	40.0
30	32.839548407259414	37.0	31.0	39.0	18.0	40.0
31	33.59974998325781	38.0	33.0	39.0	22.0	40.0
32	33.49275621135344	38.0	33.0	39.0	22.0	40.0
33	33.322398821349644	38.0	33.0	39.0	21.0	40.0
34	33.202061521976916	38.0	33.0	39.0	19.0	40.0
35	33.10308167957676	38.0	33.0	39.0	19.0	40.0
36	32.43973368752372	36.0	31.0	38.0	17.0	40.0
37	32.17159631225305	36.0	31.0	38.0	13.0	40.0
38	31.4613478581155	36.0	30.0	38.0	9.0	40.0
39	30.370605174453647	35.0	28.0	38.0	4.0	39.0
40	28.452022456860952	34.0	22.0	38.0	4.0	39.0
41	26.596451771323974	32.0	17.0	38.0	4.0	39.0
42	27.34333772350827	32.0	21.0	38.0	4.0	39.0
43	27.452948858182467	33.0	21.0	38.0	4.0	39.0
44	26.3740540661205	31.0	17.0	37.0	4.0	39.0
45	27.391655691229325	33.0	22.0	38.0	4.0	39.0
46	25.296303323883297	30.0	15.0	36.0	4.0	39.0
47	24.3397381521084	29.0	10.0	36.0	4.0	39.0
48	25.032262205058373	30.0	15.0	36.0	4.0	39.0
49	23.978508605486976	28.0	9.0	36.0	4.0	39.0
50	24.642916936401992	29.0	11.0	36.0	4.0	39.0
51	24.927550952072682	30.0	4.0	36.0	4.0	39.0
52	24.822594146929482	30.0	4.0	36.0	4.0	39.0
53	24.58766770096212	30.0	4.0	36.0	4.0	39.0
54	24.228458378909302	30.0	4.0	36.0	4.0	39.0
55	23.905283835971158	30.0	4.0	36.0	4.0	39.0
56	22.641036230104696	28.0	4.0	35.0	4.0	38.0
>>END_MODULE
>>Per tile sequence quality	fail
#Tile	Base	Mean
1	1	2.844922345080292
1	2	3.6573383817396845
1	3	4.682693081355673
1	4	7.5251073243343
1	5	3.68455576934268
1	6	3.713107520512416
1	7	3.7534126592680437
1	8	3.7639167587756006
1	9	3.8060798322769145
1	10	4.504347391328459
1	11	-0.13452324204953214
1	12	-7.763181160586939
1	13	-18.73120133018996
1	14	-7.122226578357381
1	15	0.3214979920055008
1	16	-1.7769623044367577
1	17	-6.9578079495677905
1	18	-5.375703998942914
1	19	-0.7870279187959603
1	20	-1.3701780755113795
1	21	5.728891191873448
1	22	6.4384957629795
1	23	5.679308924604726
1	24	6.321967958203867
1	25	5.536095800946139
1	26	5.863936903452913
1	27	5.008226030828073
1	28	5.3664675327725355
1	29	5.926341157393562
1	30	5.88430589012383
1	31	4.926571498910626
1	32	4.686052576415932
1	33	4.938603914853992
1	34	4.86461561483619
1	35	5.193369438456809
1	36	3.9969890286703595
1	37	4.109896468770593
1	38	4.821647200034047
1	39	4.243966427440704
1	40	-9.329789587379587
1	41	-0.03357724649918481
1	42	-1.215716904223548
1	43	-8.674378621032695
1	44	-15.107716758113678
1	45	-2.082439636218858
1	46	-4.438450961010396
1	47	-8.509864394521607
1	48	-4.452781102069185
1	49	-13.094698002654662
1	50	-2.8218870437232013
1	51	3.1808479548007718
1	52	2.5488700723189908
1	53	2.559328461514834
1	54	3.068587880362074
1	55	2.565682141128537
1	56	6.116856400375223
2	1	3.250646250804202
2	2	4.130402354803657
2	3	5.207945606608192
2	4	7.940511364738342
2	5	4.133208967995877
2	6	4.30401661142151
2	7	4.018564174419552
2	8	4.1401793850382305
2	9	4.076702727899814
2	10	4.918909680890742
2	11	3.471116488590205
2	12	-0.1171373895431671
2	13	1.842872743884115
2	14	4.4021841960533905
2	15	5.133787554295061
2	16	4.499131971657523
2	17	6.934447942688099
2	18	4.565373442134526
2	19	6.240328983560939
2	20	5.227465022131714
2	21	6.909446747428998
2	22	7.82359677308051
2	23	6.508012628308428
2	24	7.420452806688711
2	25	6.6779308177811565
2	26	6.2932298327458405
2	27	6.035582933184973
2	28	6.012932179237186
2	29	6.226846207898614
2	30	6.556443937261879
2	31	5.205191027530155
2	32	5.04926806463142
2	33	5.560657786907861
2	34	5.194581944802522
2	35	5.730827350914719
2	36	6.264244920926256
2	37	6.144408253282371
2	38	6.895721274108123
2	39	7.5629899964642675
2	40	5.043947786357787
2	41	9.25345979053785
2	42	8.347835284328639
2	43	7.462405890751821
2	44	-3.2280871284840487
2	45	7.812341508562287
2	46	6.8506904531310155
2	47	3.3945968849396735
2	48	6.4718821975941125
2	49	-0.6199505279071857
2	50	6.509341912505754
2	51	9.723777247730062
2	52	9.103163001611918
2	53	9.489463141649512
2	54	9.471786533560724
2	55	9.51938584483224
2	56	10.53689007040889
3	1	3.193407193565143
3	2	4.131177492269437
3	3	5.094618086805852
3	4	7.90152437251394
3	5	4.128691369953103
3	6	4.32767041565085
3	7	4.06925089776815
3	8	4.103408801505054
3	9	3.996972572630085
3	10	4.770725198173885
3	11	4.299774774083019
3	12	4.69030150782379
3	13	5.584146391632586
3	14	4.918704313292935
3	15	1.1762505535925953
3	16	6.672459975920777
3	17	7.781782196497179
3	18	8.202778669467815
3	19	7.124215813490938
3	20	6.331151769703357
3	21	7.388881381539893
3	22	7.731633979678868
3	23	6.759399397680809
3	24	7.710511668690021
3	25	7.05895932830607
3	26	6.22811828561991
3	27	5.708656595467271
3	28	5.4039648076798805
3	29	6.439040089157242
3	30	6.34808940876346
3	31	5.046312070090046
3	32	5.305765896668824
3	33	4.999046469900861
3	34	4.73577323419525
3	35	5.00236769439751
3	36	6.1734932798149025
3	37	6.409929169882425
3	38	7.231174123949462
3	39	8.339726183272397
3	40	8.947624844711104
3	41	11.678489463768965
3	42	10.396584164012772
3	43	10.76542408225922
3	44	11.676183394491513
3	45	11.034139827482907
3	46	12.67494014428718
3	47	13.133557231813686
3	48	12.777843489166916
3	49	13.27340751293287
3	50	12.489127780780834
3	51	11.681447475184463
3	52	12.243959455358013
3	53	12.35238492543461
3	54	12.106575671946988
3	55	12.407245254274379
3	56	11.68394818149578
4	1	3.1251478078996158
4	2	4.01864772086472
4	3	5.080045444578339
4	4	7.623760235700523
4	5	3.9555507016004086
4	6	4.229344980435883
4	7	3.7985827906156473
4	8	4.03816952534919
4	9	4.102271268826712
4	10	4.777759843495176
4	11	4.561554428857491
4	12	5.480109252072076
4	13	5.5628828563856985
4	14	5.0530500790011885
4	15	6.0062895940326015
4	16	7.305983191478024
4	17	7.967003302888511
4	18	8.75879801781166
4	19	7.488556422914659
4	20	6.529656447190039
4	21	7.034313158984833
4	22	7.693317874883526
4	23	6.7511493424349
4	24	7.324269126887284
4	25	6.889661319614046
4	26	5.397342633104376
4	27	5.099613454553381
4	28	4.577657935089217
4	29	5.384015167115354
4	30	5.205861893847068
4	31	4.137529198776178
4	32	4.403538872827141
4	33	4.267050496713537
4	34	4.271069229480929
4	35	4.542746313004333
4	36	4.924579782626299
4	37	5.28294952492945
4	38	6.039249847670828
4	39	6.705208541413192
4	40	-0.110015050198907
4	41	10.085799110931777
4	42	9.040346287419844
4	43	9.269475448674616
4	44	10.677705726114265
4	45	9.32781478512771
4	46	12.23394277153692
4	47	12.95607055221539
4	48	11.473180734728825
4	49	11.732378220325998
4	50	11.703076758117732
4	51	11.467252960489056
4	52	10.872080849028062
4	53	10.94636434940396
4	54	11.339737947587228
4	55	11.538300819378613
4	56	10.618537029188957
5	1	3.267350855536975
5	2	4.143894079562983
5	3	5.076632475295064
5	4	7.955911848448686
5	5	4.171324868787835
5	6	4.153995271259326
5	7	4.008961101436199
5	8	3.978598790359044
5	9	4.1333845697929235
5	10	5.036304284200838
5	11	4.737382434433613
5	12	5.659193999464279
5	13	5.975371439763094
5	14	5.6241455940711305
5	15	6.627942720985445
5	16	7.714181528256372
5	17	8.535683300965712
5	18	9.238572569559008
5	19	7.888654966393968
5	20	7.2750417281591275
5	21	7.301714495330554
5	22	8.208075361784452
5	23	7.377825783332852
5	24	7.979991859185514
5	25	7.284868741620489
5	26	6.293727769863498
5	27	5.894643017597172
5	28	5.921418450399511
5	29	6.576896001610383
5	30	6.645029321621909
5	31	4.99240589925207
5	32	5.644706012182041
5	33	5.694152357022148
5	34	5.429916511123004
5	35	5.430298589118355
5	36	6.436969822524393
5	37	6.623020668514506
5	38	7.227323683175317
5	39	8.536326649378388
5	40	9.782993169769366
5	41	11.781083444922071
5	42	10.649573321982167
5	43	10.872729786991204
5	44	10.996529934161185
5	45	10.969416957891259
5	46	12.58379615806771
5	47	12.75256624995129
5	48	12.383806605997396
5	49	13.188165847110596
5	50	12.388687006639579
5	51	11.880378471232696
5	52	11.642606734717628
5	53	11.71511164546703
5	54	11.67603559696472
5	55	11.908511846634298
5	56	11.753433438360709
6	1	3.1292562501689147
6	2	4.051334951207949
6	3	5.076632475295064
6	4	7.851965529022692
6	5	4.12780905693559
6	6	4.092581505646777
6	7	3.9029552550370497
6	8	3.9812695317887545
6	9	4.065127859721166
6	10	5.051531496531432
6	11	4.376726043256362
6	12	5.1920932819516565
6	13	6.037981059747146
6	14	5.5517299682406716
6	15	6.609805596350839
6	16	7.458600874522649
6	17	8.365805543857025
6	18	9.132872330388135
6	19	7.507669050901011
6	20	7.070245023402283
6	21	7.115812289643596
6	22	7.758035499972067
6	23	6.968059639298836
6	24	7.581001691765902
6	25	6.950747030220011
6	26	6.221684187615292
6	27	5.443460450496456
6	28	5.309978110245378
6	29	6.424597303762919
6	30	6.229031447585239
6	31	5.180287908287411
6	32	4.964025703917365
6	33	5.412674812509792
6	34	5.297548719467407
6	35	5.055876585397915
6	36	6.135282339133479
6	37	5.871731803247435
6	38	7.097347600262747
6	39	8.062462711031323
6	40	4.240593488663869
6	41	11.834604571682629
6	42	11.012913741859926
6	43	11.24267398045387
6	44	11.772586272189354
6	45	11.149207019012703
6	46	13.195382658200582
6	47	13.704386606050146
6	48	12.422990767570607
6	49	13.696496965898795
6	50	12.966377678975483
6	51	12.571728457945426
6	52	12.22898728215318
6	53	12.851638352881327
6	54	12.401892625930966
6	55	12.80744886497073
6	56	11.651533358637085
7	1	3.0650025243044396
7	2	3.969153026239148
7	3	5.0104846153728815
7	4	7.866266975318851
7	5	4.045509666288794
7	6	3.7562038534920177
7	7	3.5067141567329614
7	8	3.9149307901398274
7	9	3.9518615797123573
7	10	4.247434881011273
7	11	4.027471216151156
7	12	5.232795139377686
7	13	5.619641730769835
7	14	5.180538425769491
7	15	6.069316645466181
7	16	6.880743154825122
7	17	7.819223060926259
7	18	8.467062184290196
7	19	7.136462825220075
7	20	6.692137689139013
7	21	6.679324513415718
7	22	7.194347077682956
7	23	6.3344350889721355
7	24	6.728553266540143
7	25	6.309722142179485
7	26	6.009379657845084
7	27	5.773362807151617
7	28	5.163307528056112
7	29	5.283286093132272
7	30	6.088718695217569
7	31	4.37542776586028
7	32	4.714690119158533
7	33	4.656885957065995
7	34	4.724184061953274
7	35	4.92896960353168
7	36	6.1466618266894635
7	37	6.295871000075863
7	38	7.061241587488357
7	39	7.6240808007457375
7	40	6.829507665302881
7	41	10.435382781799365
7	42	10.28969224564085
7	43	10.068296181467009
7	44	11.07531000759791
7	45	9.506258630494973
7	46	11.35625190421776
7	47	12.079880328977982
7	48	11.43221182329339
7	49	12.085587341054804
7	50	11.07604657259563
7	51	10.554064556616595
7	52	10.320786709508003
7	53	10.823006093869505
7	54	11.293178140944551
7	55	11.18639314218973
7	56	10.06305967829056
8	1	3.025468262267431
8	2	3.89085677174662
8	3	4.885792780638575
8	4	7.757035383552434
8	5	3.936753317341754
8	6	3.9416525124161907
8	7	3.4470860342391276
8	8	3.5570580958100706
8	9	3.6942651202713606
8	10	4.641723056375874
8	11	4.228216753324055
8	12	5.041034661758658
8	13	5.896025132914367
8	14	5.174731552570215
8	15	5.940570395429049
8	16	6.647280119805668
8	17	7.971523533198809
8	18	7.729084342486647
8	19	7.246600348610929
8	20	6.7198057320296805
8	21	6.84695156661321
8	22	7.635610035475452
8	23	6.343286486483045
8	24	7.091861550616539
8	25	6.4453891157738035
8	26	5.934142779002304
8	27	5.37588159409432
8	28	5.442572089793121
8	29	5.876433685730365
8	30	6.034288541060679
8	31	4.649969602079722
8	32	5.001603120019915
8	33	5.002506297458659
8	34	5.023186052032578
8	35	5.17761070609502
8	36	5.877540728878547
8	37	5.41229063948537
8	38	6.3860700545332385
8	39	7.546244937734478
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115	22	5.239431093942763
115	23	4.522270664912831
115	24	5.407248134266165
115	25	5.39073859762804
115	26	4.460263295868696
115	27	4.429146125630847
115	28	4.508502406650987
115	29	4.126231118456712
115	30	4.979595377173094
115	31	3.4463361848764293
115	32	3.9634565039595273
115	33	3.667950452859742
115	34	4.08909504601953
115	35	4.17675779125857
115	36	5.577677195252381
115	37	5.197377423569982
115	38	5.440912478517202
115	39	7.156654763313391
115	40	8.89435126245959
115	41	9.19102915268822
115	42	8.533415481082024
115	43	6.0652311639681535
115	44	-1.1123299289279664
115	45	6.1530860113179635
115	46	2.851480476267408
115	47	-7.521058740213158
115	48	2.414473849682974
115	49	-2.4224447438092263
115	50	3.3755270412607175
115	51	4.719488550703936
115	52	4.483841292904735
115	53	5.049382634529898
115	54	5.003284003605682
115	55	4.533305300092476
115	56	6.839769892841787
116	1	2.861653639062773
116	2	3.559320286091257
116	3	4.5079120961481465
116	4	6.199403604673236
116	5	3.6680039956819
116	6	3.7192112114502294
116	7	3.6144280262075767
116	8	3.2865722233741934
116	9	3.4727644509757525
116	10	4.005765601869889
116	11	3.147485231309652
116	12	2.564640898870195
116	13	3.7988144675983477
116	14	3.9881066113596972
116	15	-3.878882591076504
116	16	3.9269009728862336
116	17	5.306876812747301
116	18	5.368587254827016
116	19	5.407195117251721
116	20	4.593127970733054
116	21	5.855399162575726
116	22	6.358232227147248
116	23	5.681660640818997
116	24	6.292921570152739
116	25	5.873647861839434
116	26	5.235687585630135
116	27	4.596532077072506
116	28	4.0650170085731965
116	29	5.049528002144388
116	30	5.274493309339686
116	31	4.080851263379962
116	32	4.159357904579082
116	33	4.515259092220063
116	34	3.753025782867212
116	35	3.8857254631588027
116	36	5.175521988601421
116	37	4.460715081437542
116	38	5.65950444547423
116	39	6.917993427297084
116	40	8.399924616268269
116	41	7.300007415284526
116	42	5.110740800788658
116	43	1.974892526461204
116	44	0.22471897361115367
116	45	4.844208360334353
116	46	1.0819604995669394
116	47	-6.354202323575175
116	48	0.6741577174051798
116	49	-2.5322967388315973
116	50	1.8265583102908742
116	51	4.972712093821308
116	52	4.614053901128411
116	53	4.902966261313772
116	54	4.489132185977468
116	55	3.960878177319831
116	56	6.972127117020346
117	1	2.224657193565143
117	2	3.035179290830591
117	3	4.070382475295062
117	4	6.105031566758541
117	5	3.165426981463888
117	6	3.1299635811184814
117	7	3.1572005380559176
117	8	3.0749016072604505
117	9	3.253807105004185
117	10	4.1581352701163325
117	11	0.5527873640110741
117	12	-6.865453887859665
117	13	-7.954117996856624
117	14	-0.7424917298725333
117	15	-6.683236856479347
117	16	-6.8277198801943335
117	17	0.7685178080079673
117	18	-1.9342267262156412
117	19	0.5764190509010092
117	20	-0.42680686339016916
117	21	4.594232100964351
117	22	5.260276066009801
117	23	4.260748318544117
117	24	4.759657352143261
117	25	4.893671558521898
117	26	4.098217206483216
117	27	4.176597242949281
117	28	4.196770563075564
117	29	4.745469945272351
117	30	4.81157861739656
117	31	3.830169983759113
117	32	4.361431364294724
117	33	4.636141793641869
117	34	4.091888342108916
117	35	4.3140133778507455
117	36	5.925966301397633
117	37	6.390010105134223
117	38	7.051003260640112
117	39	7.926731578955852
117	40	9.684225564135563
117	41	8.390854571682635
117	42	7.227654307897662
117	43	-2.6054392270932993
117	44	0.8288362721893527
117	45	5.947674000144779
117	46	-1.585041870101307
117	47	-0.7208492430064553
117	48	-0.541985647523731
117	49	-1.2592813359879962
117	50	1.4120144714283143
117	51	6.282931288134105
117	52	6.592430678379596
117	53	6.333949673636045
117	54	7.0318454561196475
117	55	7.408863959310352
117	56	9.750000339769162
118	1	1.6331235056218816
118	2	2.5486987943766906
118	3	3.4596111986993208
118	4	5.582247878815281
118	5	2.61476208784687
118	6	2.713429893175217
118	7	2.7084416728076945
118	8	2.7595647278278292
118	9	2.758771644011283
118	10	3.35919909990357
118	11	-0.9442427778328977
118	12	-5.743113462327752
118	13	-7.124818351466555
118	14	-8.552110524198774
118	15	-9.13514288484814
118	16	-9.132861724165963
118	17	-1.1293102061764309
118	18	-6.475317151747554
118	19	-1.252614637042253
118	20	-1.3034469343121557
118	21	3.3977338740139977
118	22	4.84702251991051
118	23	3.4251986731540427
118	24	3.9304463592354573
118	25	3.872572267741756
118	26	4.142144511447757
118	27	4.019106108197509
118	28	3.4747847474727287
118	29	4.070602923995754
118	30	4.527358759240531
118	31	3.0550370050357074
118	32	3.7531866834436585
118	33	3.4964698078262657
118	34	3.2514628101940204
118	35	3.190387491325925
118	36	5.212092187922455
118	37	4.75512535336118
118	38	5.91062205496635
118	39	6.634843281083512
118	40	8.055768117327052
118	41	6.72254783409398
118	42	5.9316879958409245
118	43	-0.49941085829897247
118	44	1.7470543572957347
118	45	5.658533929222791
118	46	-1.3165135013069786
118	47	0.536154303092836
118	48	-0.8909661439776322
118	49	-1.123066796980904
118	50	1.6852857480240573
118	51	5.679074905155382
118	52	5.290568976251937
118	53	4.446981588529663
118	54	5.592927016403337
118	55	5.605495164984113
118	56	7.572207786577671
119	1	1.971888206223369
119	2	2.97782169589388
119	3	3.950050196814054
119	4	6.427341693340821
119	5	2.9694617915904757
119	6	3.071181935548857
119	7	2.9961245886888292
119	8	2.9573383161212092
119	9	2.8651995100674768
119	10	3.875223877711271
119	11	0.8212209083148778
119	12	-5.210390596720426
119	13	-2.2966021740718148
119	14	-5.8655138817712675
119	15	-6.466860274200865
119	16	-7.966643930827242
119	17	0.2681222383877149
119	18	-4.723229890772604
119	19	0.054504493938981824
119	20	-0.09476572414966355
119	21	4.566779569318786
119	22	5.262095686262967
119	23	4.242552116012469
119	24	4.434261782523006
119	25	4.475633583838356
119	26	5.477093788761696
119	27	5.003575091050553
119	28	4.649935120037593
119	29	5.223713616158424
119	30	5.4707558325864305
119	31	4.1044579584426515
119	32	3.8615895921428205
119	33	4.1749867303507315
119	34	3.807078215526637
119	35	3.9223203398760624
119	36	5.163703643169786
119	37	5.35701959880511
119	38	5.996177311273019
119	39	6.5821904397153475
119	40	8.44197872869253
119	41	6.819414698264911
119	42	5.890391649669816
119	43	-1.9086037840553267
119	44	0.5966369051007447
119	45	5.633037924195413
119	46	0.34391382610122534
119	47	-0.916893546803923
119	48	-4.518805267776898
119	49	-1.4976515891525537
119	50	1.4037075094029987
119	51	6.346459769146762
119	52	6.324234475847952
119	53	6.654361066041108
119	54	5.951307481436103
119	55	7.038927250449593
119	56	9.09106046635144
120	1	1.8216123217702744
120	2	2.816429290830591
120	3	3.7497093983719836
120	4	6.527146951373929
120	5	2.63385646864338
120	6	2.9339699913748873
120	7	2.7988672047225833
120	8	2.7918887867476343
120	9	2.7999609511580346
120	10	3.373519885500947
120	11	-0.6662831488094341
120	12	-7.192376964782742
120	13	-7.89145774044637
120	14	-6.555793011923814
120	15	-0.8859612154537047
120	16	-8.923232700707153
120	17	-0.20664244840228818
120	18	-6.917079290318206
120	19	-1.1602796670477105
120	20	-1.484979940313245
120	21	2.7181103060925587
120	22	4.238320937804673
120	23	3.31363293392873
120	24	3.453086839322747
120	25	3.0917484815988168
120	26	4.352864642380656
120	27	4.088936986539029
120	28	3.403180819485822
120	29	4.303963535015942
120	30	4.531450412268352
120	31	3.097317419656548
120	32	3.188674954038312
120	33	3.4776482038982763
120	34	3.540606290826865
120	35	3.8154556855430535
120	36	4.1767675834489175
120	37	4.355234464108584
120	38	5.080971209358058
120	39	5.280096963571239
120	40	4.994802487212489
120	41	-6.843600556522496
120	42	-4.160646974153618
120	43	-8.373387945042019
120	44	-15.705618856015775
120	45	-5.478127281906502
120	46	-8.453311100870536
120	47	-7.912836422493633
120	48	-5.866825391113476
120	49	-14.607518515475174
120	50	-5.36763296446912
120	51	-0.8768443528915384
120	52	-1.623915475466557
120	53	-1.0234221212357504
120	54	-1.1505263387521474
120	55	-0.9238283483819529
120	56	2.190865724384544
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
4	3934.0
5	222.0
6	309.0
7	471.0
8	577.0
9	894.0
10	747.0
11	458.0
12	493.0
13	430.0
14	452.0
15	598.0
16	702.0
17	938.0
18	1172.0
19	1540.0
20	2078.0
21	2715.0
22	3578.0
23	4232.0
24	4723.0
25	5361.0
26	5696.0
27	6062.0
28	6298.0
29	6719.0
30	7520.0
31	8520.0
32	9354.0
33	10533.0
34	11612.0
35	12762.0
36	14156.0
37	16066.0
38	15279.0
39	11987.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.0	0.0	100.0	0.0
2	0.0	0.0	0.0	100.0
3	0.0	0.0	100.0	0.0
4	0.0	100.0	0.0	0.0
5	0.0	0.0	0.0	100.0
6	0.0	0.0	0.0	100.0
7	99.36435475589883	0.33149541263923926	0.11886956715851509	0.18528026430341318
8	99.2192557537335	0.5909993972810679	0.10435966694198272	0.08538518204344041
9	96.65431451117833	1.2495395845657584	1.2612592501646334	0.8348866540912795
10	49.951447641583144	25.824273946916087	15.33249994419269	8.891778467308079
11	34.44036431011005	24.8649463133692	19.218362836797105	21.47632653972364
12	28.32444136884166	19.24570841797442	20.994709467151818	31.435140746032104
13	23.012143670335067	15.294550974395607	24.023930173895575	37.66937518137375
14	21.954595173783957	23.073531709712704	29.7101368395205	25.261736276982834
15	32.33016948012267	18.026114403287565	23.831495383166356	25.81222073342341
16	32.51401514305496	23.03117950550555	22.54539566256449	21.90940968887499
17	27.40903660864808	19.292389107838652	32.01219171811676	21.28638256539651
18	24.95033149541264	19.543719445498585	26.50791347634886	28.998035582739917
19	29.835287548855387	20.809045226130653	21.46677833612507	27.88888888888889
20	25.337498044299412	26.13933528530878	27.078071567466083	21.44509510292573
21	25.13700847164399	19.804560624155908	28.43246682218477	26.625964082015336
22	23.737638681161684	19.001272406634374	29.446168270196665	27.81492064200728
23	23.584726655802843	20.9651315936335	27.440453601803693	28.00968814875996
24	23.782335705316033	20.63569136319241	26.800613924933725	28.78135900655783
25	28.444253438113947	21.859930344704413	25.65748347919271	24.038332737988927
26	29.271016556383838	20.931401289890275	20.972164056174442	28.82541809755144
27	22.969606934220963	24.805924469713048	21.141666759748457	31.082801836317536
28	25.826975560479305	28.980840174799223	20.855355319042065	24.33682894567941
29	33.116432995684356	23.62201203014244	20.26956016189262	22.991994812280584
30	28.378748191876145	21.02617660692644	28.647383735541993	21.94769146565542
31	29.947977717494446	27.21959878093707	21.52561483416502	21.306808667403462
32	35.23616003394051	22.602812372653332	21.068790925381133	21.09223666802503
33	29.788958317032005	22.68530421077341	21.956495008998335	25.56924246319625
34	24.603134663020928	25.628638048747753	29.19777006134009	20.570457226891225
35	23.940330886258547	23.961018266397545	32.65027704315835	19.448373804185557
36	26.63751800851007	28.432226577768844	23.779050937559333	21.15120447616176
37	27.792935335621877	23.093222708009527	28.407934608804553	20.705907347564043
38	32.68642270919232	23.127101433870216	23.05940799006417	21.12706786687329
39	27.716802092298064	22.030602094533425	21.89815694470834	28.354438868460168
40	22.39090038853539	18.05619685793119	25.501435891660567	34.05146686187285
41	19.05968818090475	15.86303442202829	22.802194700655924	42.27508269641103
42	25.220983804784108	24.063302973134864	23.822026165378176	26.89368705670285
43	19.953097627456355	20.278279947836502	29.138238866283462	30.63038355842368
44	21.493194914684185	21.893988654454137	25.491042400053814	31.121774030807863
45	25.644339269030876	21.739422318446536	29.023938595460674	23.592299817061914
46	21.376665546971225	24.987683350128766	23.851752323368043	29.78389877953197
47	19.48656408415953	24.33661567448746	23.778094298147234	32.39872594320578
48	25.074833579055532	25.026247598623957	25.45849528660144	24.440423535719074
49	21.625735117199085	25.758618567199925	23.230542825647827	29.385103489953163
50	25.414828112678894	23.432824969660366	25.568064247325356	25.584282670335384
51	21.3023516237402	27.39249720044793	26.254759238521835	25.050391937290033
52	21.88074907874945	25.05577829595209	30.061565818389223	23.00190680690924
53	21.294388966011375	24.348999149164836	30.298687922618782	24.05792396220501
54	21.71983930709578	25.137361100231644	28.365766592437584	24.777033000234997
55	21.264303468661126	24.907629263049465	27.301990729336943	26.52607653895246
56	25.179904424026056	21.478293957673497	28.12272671314897	25.21907490515148
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	1.0
5	2.0
6	1.0
7	0.0
8	1.5
9	3.0
10	31.0
11	59.0
12	92.0
13	150.5
14	176.0
15	204.0
16	232.0
17	257.5
18	283.0
19	285.0
20	287.0
21	301.5
22	372.5
23	429.0
24	444.5
25	460.0
26	575.5
27	691.0
28	780.5
29	906.5
30	943.0
31	1133.0
32	1323.0
33	1456.5
34	1590.0
35	1961.5
36	2333.0
37	2542.5
38	2888.0
39	3024.0
40	3252.0
41	3480.0
42	3988.5
43	4497.0
44	5692.0
45	6887.0
46	10066.0
47	12869.5
48	12494.0
49	10997.0
50	9500.0
51	9383.5
52	9267.0
53	9830.5
54	10663.0
55	10932.0
56	11048.0
57	11164.0
58	11688.5
59	12213.0
60	12607.0
61	13001.0
62	12568.5
63	10739.5
64	9343.0
65	8501.0
66	7659.0
67	6577.5
68	5496.0
69	4747.0
70	3998.0
71	3441.5
72	2426.5
73	1968.0
74	1590.0
75	1212.0
76	928.0
77	644.0
78	540.0
79	361.0
80	286.0
81	195.0
82	104.0
83	71.0
84	38.0
85	22.5
86	7.0
87	4.0
88	1.0
89	1.0
90	1.0
91	1.0
92	0.5
93	0.0
94	0.5
95	1.0
96	0.5
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.001116146170502489
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.4570618568207692
16	0.05357501618411947
17	0.027903654262562225
18	0.0
19	0.04911043150210952
20	0.12500837109627877
21	0.001116146170502489
22	0.0
23	0.0
24	0.007254950108266179
25	0.01116146170502489
26	0.057481527780878186
27	0.07478179342366677
28	0.00613880393776369
29	0.16965421791637833
30	0.07533986650891801
31	0.018974484898542313
32	0.02846172734781347
33	0.14844744067683105
34	0.19476750675268434
35	0.1869544835591669
36	0.059155747036631914
37	0.1819318257919057
38	0.24611023059579884
39	0.13728597897180614
40	0.8918007902314886
41	1.1351206554010314
42	0.3097305623144407
43	0.2896399312453959
44	0.44199388351898566
45	0.24499408442529633
46	0.3169855124227069
47	0.4799428533160703
48	0.06920106257115433
49	0.26508471549434115
50	0.21150969931022168
51	0.32814697412773175
52	0.1981159452641918
53	0.30135946603567204
54	0.25782976538607494
55	0.31252092774069695
56	0.26899122709109985
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
56	179188.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	66.97960976405184
#Duplication Level	Percentage of deduplicated	Percentage of total
1	85.92875793477559	57.554746739809424
2	8.581111598415594	11.495190124073115
3	2.3458847149038125	4.713793282671341
4	0.9869397967916091	2.6441936979885847
5	0.5218708810667205	1.7477353980535428
6	0.3334900110051961	1.3402218478402437
7	0.27174221348900723	1.2740831191139361
8	0.1731944273608602	0.9280396126351071
9	0.1244206529373885	0.7500282102301333
>10	0.6549493960666181	7.759614064327025
>50	0.045786732905508894	2.001405665684856
>100	0.02687482148801609	3.3947893569844387
>500	0.0029860912764462324	1.5480559479414508
>1k	0.0019907275176308213	2.8481029326468033
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
TCTACCGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAG	3180	1.7746724110989573	No Hit
TCTACCGGGTCACAGATCCTCTTCTGAGATGAGTTTTTGTTCAATCCGCCCAATCT	1092	0.609415809094359	No Hit
TCTACCGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTCGTCTCAAAGATTAAG	906	0.5056142152376275	No Hit
TCTACCGGGTCACAGATCCTCTTCTGAAGGAGATTGGGCGGATTGAACAAAAACTC	887	0.49501082661785384	No Hit
TCTACCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC	529	0.29522066209790837	No Hit
TCTACCGGGTACCTGGTTGATCCTGCCAGTAGCATATGCCTGTCTCAAAGATTAAG	394	0.21988079558899035	No Hit
TCTACCGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTC	391	0.2182065763332366	No Hit
TCTACCGGGTCACAGATCCTCTTCTGAAGGAGATTGGGCGCATTGAACAAAAACTC	370	0.20648704154296046	No Hit
TCTACCGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCCAAGATTAAG	345	0.19253521441167937	No Hit
TCTACCGGGTCACAGATCCTCTTCTGAGATGAGTTTTTGTCCAATCCGCCCAATCT	292	0.1629573408933634	No Hit
TCTACCGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCCACGATTAAG	267	0.1490055137620823	No Hit
TCTACCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCCCCCGACTGCTCTTCC	228	0.12724066343728374	No Hit
TCTACCGGGCTCCTACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCGAC	224	0.12500837109627877	No Hit
TCTACCGGGGGGCGCTGACCCCCCTTCCCGGGGGGGGATGCGTGCATTTATCAGAT	200	0.1116146170502489	No Hit
TCTACCGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGC	186	0.10380159385673147	No Hit
TCTACCGGTCACAGATCCTCTTCTGAGATGAGTTTTTGTTCAATCCGCCCAATCTC	183	0.10212737460097775	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.002232292341004978	0.0	0.0	0.0	0.0
12	0.005022657767261201	0.0	0.0	0.0	0.0
13	0.007254950108266179	0.0	0.0	0.0	0.0
14	0.022880996495301023	0.0	0.0	0.0	0.0
15	0.025113288836306	0.0	0.0	0.0	0.0
16	0.029577873518315957	0.0	0.0	0.0	0.0
17	0.03292631202982343	0.0	0.0	0.0	0.0
18	0.03404245820032591	0.0	0.0	0.0	0.0
19	0.036832823626582134	0.0	0.0	0.0	0.0
20	0.03906511596758711	0.0	0.0	0.0	0.0
21	0.040181262138089605	0.0	0.0	0.0	0.0
22	0.04073933522334085	0.0	0.0	0.0	0.0
23	0.04129740830859209	0.0	0.0	0.0	0.0
24	0.0452039199053508	0.0	0.0	0.0	0.0
25	0.04687813916110454	0.0	0.0	0.0	0.0
26	0.050226577672612	0.0	0.0	0.0	0.0
27	0.05469116235462196	0.0	0.0	0.0	0.0
28	0.05803960086612943	0.0	0.0	0.0	0.0
29	0.06138803937763689	0.0	0.0	0.0	0.0
30	0.06417840480389311	0.0	0.0	0.0	0.0
31	0.07310757416791303	0.0	0.0	0.0	0.0
32	0.07478179342366677	0.0	0.0	0.0	0.0
33	0.07589793959416925	0.0	0.0	0.0	0.0
34	0.07868830502042548	0.0	0.0	0.0	0.0
35	0.07980445119092797	0.0	0.0	0.0	0.0
36	0.0814786704466817	0.0	0.0	0.0	0.0
37	0.08482710895818917	0.0	0.0	0.0	0.0
38	0.08594325512869165	0.0	0.0	0.0	0.0
39	0.08817554746969664	0.0	0.0	0.0	0.0
40	0.08929169364019912	0.0	0.0	0.0	0.0
41	0.08984976672545036	0.0	0.0	0.0	0.0
42	0.09264013215170659	0.0	0.0	0.0	0.0
43	0.09710471683371655	0.0	0.0	0.0	0.0
44	0.10380159385673147	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TACCAAT	15	0.008059583	50.555336	38
AGGGCAC	15	0.008141283	50.42655	37
ATGCTCG	120	0.0	50.284225	36
ACAATTA	40	2.562956E-9	50.21336	43
GCCACTA	15	0.008278817	50.21336	46
GATTCGT	20	4.1561783E-4	50.0441	9
CGATTAA	50	7.2759576E-12	50.030052	49
ACGATTA	50	7.2759576E-12	50.016003	48
GATTACG	25	2.0936859E-5	49.945896	50
CAACTAG	55	0.0	49.87598	16
GCAATAG	15	0.008530725	49.834126	33
CGCGATC	15	0.008568523	49.77843	25
CGGGTCG	25	2.149884E-5	49.72286	6
ACCGGTA	25	2.149884E-5	49.72286	4
CTACCGA	95	0.0	49.72286	2
TACCGCG	15	0.008606447	49.72286	3
CGGGGTA	130	0.0	49.722855	6
CGGGGTC	435	0.0	49.15133	6
ACCGGGA	4520	0.0	48.952816	4
TACCGGG	17400	0.0	48.894146	3
>>END_MODULE