FastQCFastQC Report
Fri 17 Jun 2016
SRR1526161_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526161_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3078
Sequences flagged as poor quality0
Sequence length40
%GC50

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTACCGGGGTCGACTTTTTTTTTTTTTTTTTTTTTTTTT100.3248862897985705No Hit
TCTACCGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCG60.1949317738791423No Hit
TCTACCGGGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTT60.1949317738791423No Hit
TCTACCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC60.1949317738791423No Hit
TCTACCGGGCTCGTCTTAATCAGCAACATCTTTTGATACA50.16244314489928524No Hit
TCTACCGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTT50.16244314489928524No Hit
TCTACCGGGATGAGATGGACTTAAATGAATTGACGGGTAC40.1299545159194282No Hit
TCTACCGGGAAGCAGTGGTATCAACGCCGAGTCCCCTTTT40.1299545159194282No Hit
TCTACCGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCC40.1299545159194282No Hit
TCTACCGGGGGGGTTGGGGATTTAGCTCCGTGGTAGAGCC40.1299545159194282No Hit
TCTACCGGGACAGCTTTACTCTTTCAGGTCACTCTGGGGC40.1299545159194282No Hit
TCTACCGGGAGAGTCGATTTTTTTTTTTTTTTTTTTTTTT40.1299545159194282No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGGGT405.1419192E-833.475414
ACCGGGG600.033.475414
ACCGGGC750.033.475414
ACCGGGA900.033.475414
TACCGGG2650.033.475413
CGGGAAG251.7469167E-433.475416
CCGGGGG301.1763537E-533.475415
CCGGGCT301.1763537E-533.475415
CCGGGCC251.7469167E-433.475415
CCGGGCA200.002565750233.475415
CCGGGAT251.7469167E-433.475415
CCGGGAG200.002565750233.475415
CCGGGAA357.8278754E-733.475415
CTACCGG3000.033.475412
TCTACCG3100.032.9354861
Produced by FastQC (version 0.11.5)