##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1526161_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3078
Sequences flagged as poor quality	0
Sequence length	40
%GC	50
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.86842105263158	33.0	32.0	35.0	29.0	36.0
2	18.326185834957766	17.0	12.0	24.0	4.0	34.0
3	18.680961663417804	18.0	12.0	26.0	4.0	35.0
4	23.72904483430799	27.0	17.0	33.0	4.0	35.0
5	19.406432748538013	17.0	14.0	28.0	4.0	35.0
6	18.157894736842106	16.0	11.0	28.0	4.0	33.0
7	21.79142300194932	26.0	13.0	30.0	4.0	33.0
8	20.77485380116959	25.0	9.0	30.0	4.0	32.0
9	21.209876543209877	25.0	10.0	29.0	4.0	32.0
10	16.015594541910332	16.0	4.0	24.0	4.0	30.0
11	20.089993502274204	23.0	4.0	30.0	4.0	35.0
12	20.132228719948017	23.0	4.0	30.0	4.0	34.0
13	20.652696556205328	24.0	4.0	31.0	4.0	35.0
14	20.65497076023392	24.0	4.0	31.0	4.0	35.0
15	19.091617933723196	21.0	4.0	30.0	4.0	34.0
16	18.66341780376868	20.0	4.0	30.0	4.0	35.0
17	19.509421702404158	23.0	4.0	31.0	4.0	35.0
18	18.97920727745289	21.0	4.0	31.0	4.0	35.0
19	16.85055230669266	16.0	4.0	29.0	4.0	33.0
20	18.249512670565302	19.0	4.0	31.0	4.0	35.0
21	15.182586094866796	12.0	4.0	26.0	4.0	33.0
22	15.573424301494477	11.0	4.0	28.0	4.0	33.0
23	15.765107212475634	12.0	4.0	28.0	4.0	33.0
24	15.164392462638077	10.0	4.0	27.0	4.0	33.0
25	14.915529564652372	4.0	4.0	28.0	4.0	33.0
26	13.186484730344379	4.0	4.0	24.0	4.0	33.0
27	13.094541910331383	4.0	4.0	24.0	4.0	33.0
28	12.979207277452891	4.0	4.0	24.0	4.0	33.0
29	12.462962962962964	4.0	4.0	23.0	4.0	32.0
30	11.651721897335932	4.0	4.0	20.0	4.0	31.0
31	12.258284600389864	4.0	4.0	24.0	4.0	33.0
32	11.680961663417804	4.0	4.0	22.0	4.0	32.0
33	11.683560753736192	4.0	4.0	22.0	4.0	32.0
34	11.342105263157896	4.0	4.0	20.0	4.0	31.0
35	11.173814165042236	4.0	4.0	21.0	4.0	31.0
36	11.604938271604938	4.0	4.0	20.0	4.0	35.0
37	11.475633528265107	4.0	4.0	19.0	4.0	35.0
38	11.401234567901234	4.0	4.0	19.0	4.0	35.0
39	10.94996751137102	4.0	4.0	17.0	4.0	34.0
40	10.833008447043534	4.0	4.0	17.0	4.0	32.0
>>END_MODULE
>>Per tile sequence quality	fail
#Tile	Base	Mean
1	1	-17.914294733044734
1	2	-15.280965909090913
1	3	-15.774161255411258
1	4	-20.21031746031746
1	5	-17.483987193362196
1	6	-14.543005952380955
1	7	-17.215052308802317
1	8	-16.04679834054834
1	9	-17.729243326118322
1	10	-11.409604978354979
1	11	-15.5929788961039
1	12	-15.761602633477636
1	13	-16.286710858585863
1	14	-16.481768578643578
1	15	-14.355244408369405
1	16	-14.544331709956715
1	17	-15.515444624819622
1	18	-14.808062770562774
1	19	-12.538970057720057
1	20	-13.294611291486294
1	21	-10.194349747474751
1	22	-10.459771825396825
1	23	-10.58017225829726
1	24	-10.559816919191917
1	25	-10.74387626262626
1	26	-9.629229797979798
1	27	-9.444760101010102
1	28	-9.609388528138526
1	29	-9.232995129870135
1	30	-8.685470779220777
1	31	-9.012283549783552
1	32	-8.507314213564218
1	33	-8.311079545454545
1	34	-7.9700216450216494
1	35	-7.649246933621935
1	36	-8.132589285714289
1	37	-7.845404942279943
1	38	-8.054184704184706
1	39	-7.99048971861472
1	40	-7.495896464646465
6	1	0.18570526695526723
6	2	-3.155965909090913
6	3	-4.899161255411258
6	4	-3.7103174603174587
6	5	-2.3589871933621964
6	6	-2.1680059523809554
6	7	3.909947691197683
6	8	4.703201659451661
6	9	5.395756673881678
6	10	5.340395021645021
6	11	3.407021103896099
6	12	0.9883973665223635
6	13	2.838289141414137
6	14	3.518231421356422
6	15	-1.2302444083694049
6	16	3.2056682900432847
6	17	3.6095553751803777
6	18	7.816937229437226
6	19	6.961029942279943
6	20	5.830388708513706
6	21	14.430650252525249
6	22	12.040228174603175
6	23	12.91982774170274
6	24	11.315183080808083
6	25	10.13112373737374
6	26	14.870770202020202
6	27	14.305239898989898
6	28	15.890611471861474
6	29	13.767004870129865
6	30	15.314529220779223
6	31	6.612716450216448
6	32	6.992685786435782
6	33	5.563920454545455
6	34	5.7799783549783506
6	35	7.475753066378065
6	36	5.242410714285711
6	37	3.4045950577200568
6	38	4.945815295815294
6	39	2.63451028138528
6	40	3.5041035353535346
7	1	-0.31429473304473277
7	2	-2.030965909090913
7	3	-4.774161255411258
7	4	4.914682539682541
7	5	-4.983987193362196
7	6	8.831994047619045
7	7	4.534947691197683
7	8	2.2032016594516612
7	9	5.520756673881678
7	10	5.715395021645021
7	11	5.657021103896099
7	12	6.4883973665223635
7	13	10.588289141414137
7	14	8.143231421356422
7	15	9.269755591630595
7	16	7.705668290043285
7	17	7.859555375180378
7	18	5.816937229437226
7	19	6.211029942279943
7	20	10.080388708513706
7	21	7.430650252525249
7	22	13.415228174603175
7	23	11.16982774170274
7	24	9.315183080808083
7	25	10.25612373737374
7	26	8.995770202020202
7	27	7.930239898989898
7	28	15.640611471861474
7	29	12.642004870129865
7	30	13.064529220779223
7	31	9.362716450216448
7	32	0.7426857864357821
7	33	0.31392045454545503
7	34	3.5299783549783506
7	35	0.3507530663780649
7	36	-4.757589285714289
7	37	-4.470404942279943
7	38	-4.679184704184706
7	39	-6.49048971861472
7	40	-5.995896464646465
9	1	2.13014971139971
9	2	2.4968118686868657
9	3	-2.9963834776334792
9	4	3.900793650793652
9	5	-4.706209415584418
9	6	0.4569940476190446
9	7	8.007169913419904
9	8	8.73097943722944
9	9	5.826312229437235
9	10	5.923728354978353
9	11	2.407021103896099
9	12	5.127286255411253
9	13	2.4910669191919155
9	14	-2.8151019119769103
9	15	3.644755591630595
9	16	3.344557178932174
9	17	9.373444264069267
9	18	7.080826118326115
9	19	3.1276966089466107
9	20	9.260944264069263
9	21	16.02787247474747
9	22	12.095783730158733
9	23	13.41982774170274
9	24	11.884627525252526
9	25	13.589457070707072
9	26	4.926325757575759
9	27	-0.11142676767676818
9	28	-3.9427218614718598
9	29	0.8781159812409758
9	30	-1.6854707792207773
9	31	-3.1233946608946628
9	32	-1.507314213564218
9	33	-3.7555239898989896
9	34	-3.08113275613276
9	35	0.2396419552669542
9	36	1.3118551587301557
9	37	1.7101506132756121
9	38	0.1680375180375151
9	39	-0.21271194083694134
9	40	-1.3847853535353547
10	1	-0.4809613997114006
10	2	-1.780965909090913
10	3	7.39250541125541
10	4	4.456349206349209
10	5	-7.483987193362196
10	6	0.9569940476190446
10	7	7.451614357864351
10	8	6.953201659451661
10	9	1.6040900072150102
10	10	4.423728354978353
10	11	3.073687770562767
10	12	1.4050640331890314
10	13	3.546622474747469
10	14	6.18489808802309
10	15	2.4780889249639273
10	16	11.455668290043285
10	17	11.651222041847046
10	18	9.858603896103894
10	19	14.12769660894661
10	20	9.372055375180373
10	21	7.4723169191919165
10	22	13.873561507936508
10	23	8.586494408369408
10	24	8.106849747474751
10	25	7.2561237373737395
10	26	3.0374368686868696
10	27	3.38857323232323
10	28	3.890611471861474
10	29	0.26700487012986507
10	30	1.3145292207792227
10	31	0.154383116883114
10	32	-2.673980880230884
10	33	-2.144412878787879
10	34	-0.6366883116883155
10	35	0.517419733044731
10	36	-3.632589285714289
10	37	-2.3454049422799432
10	38	-4.387518037518039
10	39	1.342843614718614
10	40	-3.8292297979797985
13	1	1.7968163780663815
13	2	13.274589646464644
13	3	9.448060966810964
13	4	-9.765873015873014
13	5	11.182679473304468
13	6	5.790327380952377
13	7	-3.215052308802317
13	8	-3.0467983405483388
13	9	-5.39590999278499
13	10	-1.409604978354979
13	11	0.40702110389609913
13	12	-1.7616026334776365
13	13	-2.1755997474747524
13	14	-0.7039908008657996
13	15	0.8669778138528166
13	16	0.5667794011543954
13	17	-0.18211129148629013
13	18	0.3030483405483366
13	19	-0.9834145021645018
13	20	2.3720553751803735
13	21	6.361205808080806
13	22	-0.23754960317460316
13	23	1.5309388528138506
13	24	4.884627525252526
13	25	0.03390151515151807
13	26	1.0374368686868678
13	27	-2.2225378787878807
13	28	-2.9427218614718598
13	29	-2.6774395743145796
13	30	-4.352137445887443
13	31	-3.9011724386724413
13	32	-4.618425324675329
13	33	-2.8666351010101003
13	34	-3.8589105339105387
13	35	-4.09369137806638
13	36	-1.5770337301587336
13	37	-2.289849386724388
13	38	-1.498629148629151
13	39	-1.4349341630591645
13	40	-1.3847853535353547
14	1	-0.9809613997114006
14	2	-4.114299242424247
14	3	4.559172077922074
14	4	2.6230158730158735
14	5	-4.650653860028864
14	6	-1.0430059523809554
14	7	10.118281024531015
14	8	11.953201659451661
14	9	6.937423340548346
14	10	9.757061688311689
14	11	2.907021103896099
14	12	9.071730699855696
14	13	7.713289141414137
14	14	5.18489808802309
14	15	2.4780889249639273
14	16	11.455668290043285
14	17	14.484555375180378
14	18	11.358603896103894
14	19	16.627696608946607
14	20	16.03872204184704
14	21	14.972316919191917
14	22	14.040228174603175
14	23	14.753161075036072
14	24	14.273516414141415
14	25	11.089457070707072
14	26	9.03743686868687
14	27	15.055239898989898
14	28	6.057278138528142
14	29	10.600338203463197
14	30	8.147862554112555
14	31	13.32104978354978
14	32	12.65935245310245
14	33	13.688920454545455
14	34	10.196645021645018
14	35	16.850753066378065
14	36	4.367410714285711
14	37	10.487928391053389
14	38	6.445815295815294
14	39	5.676176948051948
14	40	6.504103535353535
15	1	-3.8142947330447328
15	2	5.219034090909087
15	3	-4.774161255411258
15	4	-2.2103174603174587
15	5	8.016012806637804
15	6	7.956994047619045
15	7	8.784947691197683
15	8	10.453201659451661
15	9	8.770756673881678
15	10	-0.9096049783549791
15	11	15.9070211038961
15	12	13.738397366522364
15	13	13.713289141414137
15	14	14.018231421356422
15	15	12.144755591630595
15	16	5.955668290043285
15	17	9.984555375180378
15	18	10.691937229437226
15	19	8.961029942279943
15	20	11.205388708513706
15	21	19.305650252525247
15	22	18.040228174603175
15	23	17.41982774170274
15	24	18.940183080808083
15	25	8.75612373737374
15	26	16.870770202020203
15	27	20.555239898989896
15	28	16.890611471861476
15	29	17.267004870129867
15	30	16.81452922077922
15	31	18.487716450216446
15	32	15.992685786435782
15	33	18.188920454545453
15	34	19.52997835497835
15	35	21.850753066378065
15	36	14.367410714285711
15	37	15.654595057720057
15	38	9.445815295815294
15	39	11.00951028138528
15	40	9.504103535353535
16	1	2.114276695526698
16	2	0.4333198051948024
16	3	-2.0598755411255425
16	4	4.075396825396826
16	5	-3.341130050505054
16	6	-4.400148809523813
16	7	3.9278048340548253
16	8	5.524630230880234
16	9	3.4136138167388204
16	10	3.4475378787878785
16	11	2.5498782467532415
16	12	0.09554022366522119
16	13	0.5704319985569946
16	14	1.946802849927849
16	15	2.3590413059163104
16	16	-1.1157602813852883
16	17	3.7702696608946624
16	18	0.04908008658008356
16	19	0.4610299422799429
16	20	-3.723182720057723
16	21	1.5199359668109622
16	22	0.11165674603174658
16	23	2.2769705988455975
16	24	2.1544687950937984
16	25	2.541838023088024
16	26	3.085055916305917
16	27	3.555239898989898
16	28	1.9620400432900453
16	29	2.0527191558441515
16	30	0.4573863636363651
16	31	2.8448593073593056
16	32	3.778400072150067
16	33	4.831777597402597
16	34	4.744264069264066
16	35	5.06503878066378
16	36	2.29598214285714
16	37	1.2974522005771991
16	38	3.088672438672436
16	39	-0.13334686147186225
16	40	-2.781610750360752
17	1	1.5428481240981213
17	2	7.004748376623372
17	3	-2.3455898268398307
17	4	4.789682539682541
17	5	-5.055415764790769
17	6	7.456994047619045
17	7	6.927804834054825
17	8	5.238915945165946
17	9	7.270756673881678
17	10	1.1618235930735938
17	11	7.121306818181814
17	12	5.2383973665223635
17	13	2.8561462842712793
17	14	4.232517135642137
17	15	2.644755591630595
17	16	4.169954004329
17	17	5.62741251803752
17	18	4.477651515151511
17	19	5.3181727994228005
17	20	9.562531565656563
17	21	5.091364538239533
17	22	11.82594246031746
17	23	11.848399170274167
17	24	8.725897366522368
17	25	3.970409451659455
17	26	6.656484487734486
17	27	3.555239898989898
17	28	2.962040043290047
17	29	5.909862012987007
17	30	0.7431006493506516
17	31	-2.1551406926406944
17	32	-2.2215999278499314
17	33	-1.4539366883116873
17	34	-0.2557359307359359
17	35	-2.649246933621935
17	36	-1.4183035714285754
17	37	0.15459505772005677
17	38	0.6601010101010072
17	39	0.7237959956709936
17	40	0.7898178210678211
18	1	2.1857052669552672
18	2	-0.5309659090909129
18	3	-2.149161255411258
18	4	8.664682539682541
18	5	6.766012806637804
18	6	6.581994047619045
18	7	10.409947691197683
18	8	10.953201659451661
18	9	9.270756673881678
18	10	12.215395021645021
18	11	10.2820211038961
18	12	12.863397366522364
18	13	9.088289141414137
18	14	10.268231421356422
18	15	7.019755591630595
18	16	0.3306682900432847
18	17	6.109555375180378
18	18	6.691937229437226
18	19	6.961029942279943
18	20	8.080388708513706
18	21	6.930650252525249
18	22	9.790228174603175
18	23	9.29482774170274
18	24	8.815183080808083
18	25	6.3811237373737395
18	26	7.995770202020202
18	27	10.930239898989898
18	28	12.390611471861474
18	29	9.517004870129865
18	30	7.314529220779223
18	31	6.987716450216448
18	32	3.992685786435782
18	33	4.813920454545455
18	34	6.2799783549783506
18	35	5.850753066378065
18	36	5.742410714285711
18	37	5.904595057720057
18	38	6.570815295815294
18	39	6.25951028138528
18	40	6.254103535353535
19	1	-1.8142947330447328
19	2	-5.280965909090913
19	3	-7.774161255411258
19	4	-0.7103174603174587
19	5	-5.983987193362196
19	6	4.956994047619045
19	7	4.784947691197683
19	8	10.203201659451661
19	9	4.520756673881678
19	10	5.090395021645021
19	11	15.1570211038961
19	12	16.238397366522364
19	13	16.213289141414137
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102	4	4.289682539682541
102	5	-0.23398719336219642
102	6	-1.0430059523809554
102	7	-0.21505230880231707
102	8	3.4532016594516612
102	9	6.770756673881678
102	10	1.090395021645021
102	11	-0.5929788961039009
102	12	5.7383973665223635
102	13	8.463289141414137
102	14	4.518231421356422
102	15	6.644755591630595
102	16	11.705668290043285
102	17	4.984555375180378
102	18	6.441937229437226
102	19	6.961029942279943
102	20	4.705388708513706
102	21	1.3056502525252487
102	22	-2.2097718253968246
102	23	3.66982774170274
102	24	4.690183080808083
102	25	-2.4938762626262605
102	26	9.120770202020202
102	27	4.055239898989898
102	28	-0.35938852813852584
102	29	2.267004870129865
102	30	3.5645292207792227
102	31	4.487716450216448
102	32	4.992685786435782
102	33	5.188920454545455
102	34	5.5299783549783506
102	35	-3.149246933621935
102	36	-2.632589285714289
102	37	1.1545950577200568
102	38	0.19581529581529367
102	39	-2.49048971861472
102	40	6.504103535353535
103	1	-7.647628066378065
103	2	1.385700757575755
103	3	0.89250541125541
103	4	-1.5436507936507908
103	5	-1.8173205266955286
103	6	-10.209672619047621
103	7	-9.215052308802317
103	8	-15.046798340548339
103	9	-11.729243326118322
103	10	-7.409604978354979
103	11	-6.592978896103901
103	12	-13.428269300144304
103	13	-8.286710858585863
103	14	-7.815101911976912
103	15	-12.021911075036073
103	16	-4.877665043290049
103	17	-15.515444624819622
103	18	-14.808062770562774
103	19	-12.538970057720057
103	20	-13.294611291486294
103	21	-10.194349747474751
103	22	-10.459771825396825
103	23	-10.58017225829726
103	24	-10.559816919191917
103	25	-10.74387626262626
103	26	-9.629229797979798
103	27	-9.444760101010102
103	28	-9.609388528138526
103	29	-9.232995129870135
103	30	-8.685470779220777
103	31	-9.012283549783552
103	32	-8.507314213564218
103	33	-8.311079545454545
103	34	-7.9700216450216494
103	35	-7.649246933621935
103	36	-8.132589285714289
103	37	-7.845404942279943
103	38	-8.054184704184706
103	39	-7.99048971861472
103	40	-7.495896464646465
104	1	1.019038600288603
104	2	-8.280965909090913
104	3	-5.774161255411258
104	4	-6.543650793650791
104	5	-7.1506538600288625
104	6	-5.543005952380955
104	7	-2.215052308802317
104	8	-0.7134650072150066
104	9	-6.062576659451656
104	10	3.923728354978353
104	11	-10.926312229437235
104	12	-4.4282693001443025
104	13	-1.286710858585863
104	14	-7.815101911976912
104	15	-3.355244408369405
104	16	-2.5443317099567153
104	17	-8.515444624819622
104	18	-11.474729437229442
104	19	-12.205636724386725
104	20	-9.294611291486294
104	21	-2.8610164141414174
104	22	-10.126438492063492
104	23	-8.58017225829726
104	24	-5.559816919191917
104	25	-3.4105429292929266
104	26	-8.962563131313132
104	27	-7.444760101010102
104	28	-1.9427218614718598
104	29	-2.566328463203469
104	30	-2.6854707792207773
104	31	-1.345616883116886
104	32	-0.8406475468975518
104	33	0.022253787878788955
104	34	-0.6366883116883155
104	35	-0.6492469336219351
104	36	2.200744047619045
104	37	2.1545950577200568
104	38	2.2791486291486276
104	39	2.342843614718614
104	40	2.8374368686868685
105	1	0.9357052669552672
105	2	-6.280965909090913
105	3	-5.024161255411258
105	4	-1.2103174603174587
105	5	-5.483987193362196
105	6	-8.043005952380955
105	7	-3.965052308802317
105	8	-0.04679834054833876
105	9	-3.229243326118322
105	10	5.340395021645021
105	11	-1.8429788961039009
105	12	2.7383973665223635
105	13	0.9632891414141369
105	14	1.0182314213564219
105	15	5.144755591630595
105	16	0.4556682900432847
105	17	1.4845553751803777
105	18	-0.05806277056277409
105	19	-6.038970057720057
105	20	0.7053887085137056
105	21	-6.694349747474751
105	22	-4.459771825396825
105	23	-1.8301722582972602
105	24	-1.309816919191917
105	25	1.5061237373737395
105	26	2.8707702020202017
105	27	2.055239898989898
105	28	4.140611471861474
105	29	1.767004870129865
105	30	4.314529220779223
105	31	5.987716450216448
105	32	6.492685786435782
105	33	4.938920454545455
105	34	5.0299783549783506
105	35	3.100753066378065
105	36	3.617410714285711
105	37	6.654595057720057
105	38	5.195815295815294
105	39	5.25951028138528
105	40	5.004103535353535
106	1	1.6857052669552672
106	2	7.219034090909087
106	3	5.225838744588742
106	4	4.289682539682541
106	5	10.516012806637804
106	6	5.456994047619045
106	7	5.284947691197683
106	8	10.953201659451661
106	9	6.770756673881678
106	10	0.09039502164502089
106	11	11.4070211038961
106	12	6.2383973665223635
106	13	12.713289141414137
106	14	10.018231421356422
106	15	12.644755591630595
106	16	8.955668290043285
106	17	4.484555375180378
106	18	8.191937229437226
106	19	14.961029942279943
106	20	-13.294611291486294
106	21	-10.194349747474751
106	22	-10.459771825396825
106	23	-10.58017225829726
106	24	-10.559816919191917
106	25	-10.74387626262626
106	26	-9.629229797979798
106	27	-9.444760101010102
106	28	-9.609388528138526
106	29	-9.232995129870135
106	30	-8.685470779220777
106	31	-9.012283549783552
106	32	-8.507314213564218
106	33	-8.311079545454545
106	34	-7.9700216450216494
106	35	-7.649246933621935
106	36	-8.132589285714289
106	37	-7.845404942279943
106	38	-8.054184704184706
106	39	-7.99048971861472
106	40	-7.495896464646465
107	1	-0.31429473304473277
107	2	-6.280965909090913
107	3	-7.774161255411258
107	4	-6.210317460317459
107	5	2.0160128066378036
107	6	3.4569940476190446
107	7	4.784947691197683
107	8	5.453201659451661
107	9	6.270756673881678
107	10	5.090395021645021
107	11	2.907021103896099
107	12	8.738397366522364
107	13	10.213289141414137
107	14	10.018231421356422
107	15	12.144755591630595
107	16	15.455668290043285
107	17	13.484555375180378
107	18	11.191937229437226
107	19	-0.5389700577200571
107	20	-5.294611291486294
107	21	2.3056502525252487
107	22	0.040228174603175404
107	23	1.4198277417027398
107	24	3.440183080808083
107	25	3.2561237373737395
107	26	-2.1292297979797983
107	27	1.555239898989898
107	28	4.890611471861474
107	29	-9.232995129870135
107	30	-8.685470779220777
107	31	-9.012283549783552
107	32	-8.507314213564218
107	33	-8.311079545454545
107	34	-7.9700216450216494
107	35	-7.649246933621935
107	36	-8.132589285714289
107	37	-7.845404942279943
107	38	-8.054184704184706
107	39	-7.99048971861472
107	40	-7.495896464646465
108	1	0.6857052669552672
108	2	-8.280965909090913
108	3	-6.774161255411258
108	4	3.7896825396825413
108	5	11.516012806637804
108	6	14.456994047619045
108	7	5.784947691197683
108	8	5.953201659451661
108	9	9.270756673881678
108	10	-2.409604978354979
108	11	12.4070211038961
108	12	10.238397366522364
108	13	11.713289141414137
108	14	6.518231421356422
108	15	8.644755591630595
108	16	14.455668290043285
108	17	5.484555375180378
108	18	1.191937229437226
108	19	12.461029942279943
108	20	-4.294611291486294
108	21	4.805650252525249
108	22	-4.459771825396825
108	23	10.41982774170274
108	24	-0.5598169191919169
108	25	14.25612373737374
108	26	19.370770202020203
108	27	2.555239898989898
108	28	12.390611471861474
108	29	17.767004870129867
108	30	-8.685470779220777
108	31	-9.012283549783552
108	32	-8.507314213564218
108	33	-8.311079545454545
108	34	-7.9700216450216494
108	35	-7.649246933621935
108	36	-8.132589285714289
108	37	-7.845404942279943
108	38	-8.054184704184706
108	39	-7.99048971861472
108	40	-7.495896464646465
111	1	-28.314294733044733
111	2	-15.280965909090913
111	3	-15.774161255411258
111	4	-20.21031746031746
111	5	-17.483987193362196
111	6	-14.543005952380955
111	7	-17.215052308802317
111	8	-16.04679834054834
111	9	-17.729243326118322
111	10	-11.409604978354979
111	11	-15.5929788961039
111	12	-15.761602633477636
111	13	-16.286710858585863
111	14	-16.481768578643578
111	15	-14.355244408369405
111	16	-14.544331709956715
111	17	-15.515444624819622
111	18	-14.808062770562774
111	19	-12.538970057720057
111	20	-13.294611291486294
111	21	-10.194349747474751
111	22	-10.459771825396825
111	23	-10.58017225829726
111	24	-10.559816919191917
111	25	-10.74387626262626
111	26	-9.629229797979798
111	27	-9.444760101010102
111	28	-9.609388528138526
111	29	-9.232995129870135
111	30	-8.685470779220777
111	31	-9.012283549783552
111	32	-8.507314213564218
111	33	-8.311079545454545
111	34	-7.9700216450216494
111	35	-7.649246933621935
111	36	-8.132589285714289
111	37	-7.845404942279943
111	38	-8.054184704184706
111	39	-7.99048971861472
111	40	-7.495896464646465
112	1	-0.9809613997114006
112	2	7.052367424242419
112	3	-1.7741612554112578
112	4	-0.8769841269841265
112	5	-6.81732052669553
112	6	-3.8763392857142893
112	7	5.451614357864351
112	8	3.619868326118329
112	9	8.60409000721501
112	10	13.923728354978353
112	11	0.07368777056276699
112	12	4.905064033189031
112	13	4.713289141414137
112	14	1.851564754689754
112	15	-1.0219110750360727
112	16	3.789001623376617
112	17	11.151222041847046
112	18	-5.141396103896108
112	19	-5.872303391053391
112	20	2.7053887085137056
112	21	2.138983585858581
112	22	2.8735615079365076
112	23	2.0864944083694077
112	24	1.7735164141414153
112	25	1.9227904040404074
112	26	5.03743686868687
112	27	4.555239898989898
112	28	3.057278138528142
112	29	2.767004870129865
112	30	8.647862554112555
112	31	2.654383116883114
112	32	5.492685786435782
112	33	8.355587121212123
112	34	8.696645021645018
112	35	1.350753066378065
112	36	0.867410714285711
112	37	-2.5120716089466093
112	38	0.9458152958152937
112	39	0.6761769480519462
112	40	1.5041035353535346
>>END_MODULE
>>Per sequence quality scores	fail
#Quality	Count
4	399.0
5	46.0
6	74.0
7	136.0
8	103.0
9	38.0
10	79.0
11	86.0
12	111.0
13	102.0
14	125.0
15	120.0
16	136.0
17	130.0
18	123.0
19	122.0
20	105.0
21	126.0
22	118.0
23	148.0
24	163.0
25	135.0
26	115.0
27	70.0
28	69.0
29	33.0
30	30.0
31	19.0
32	11.0
33	3.0
34	3.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.0	0.0	100.0	0.0
2	0.0	0.0	0.0	100.0
3	0.0	0.0	100.0	0.0
4	0.0	100.0	0.0	0.0
5	0.0	0.0	0.0	100.0
6	0.0	0.0	0.0	100.0
7	98.01496908558413	0.35795639440286364	0.35795639440286364	1.269118125610153
8	96.84655396618986	1.2678803641092327	0.42262678803641096	1.4629388816644993
9	92.65518362040949	4.127396815079623	1.5599610009749756	1.6574585635359116
10	30.64673383165421	36.301592460188495	13.032174195645108	20.019499512512187
11	26.218323586744642	29.98700454840806	21.637426900584796	22.157244964262507
12	36.65908352291193	17.93955151121222	21.6769580760481	23.724406889827755
13	29.629629629629626	20.207927225471085	25.601039636127354	24.561403508771928
14	21.34502923976608	28.947368421052634	27.160493827160494	22.54710851202079
15	25.308641975308642	24.496426250812213	28.817413905133204	21.377517868745937
16	23.359324236517217	19.980506822612085	28.88239116309292	27.77777777777778
17	25.276153346328783	25.406107862248213	22.839506172839506	26.478232618583498
18	27.29044834307992	25.763482781026642	21.21507472384665	25.730994152046783
19	18.54864952814839	17.279531402538236	18.255776114546045	45.91604295476733
20	21.24756335282651	25.601039636127354	24.561403508771928	28.589993502274204
21	17.901234567901234	19.200779727095515	27.87524366471735	35.0227420402859
22	20.69525666016894	18.128654970760234	26.31578947368421	34.86029889538661
23	24.333983105912928	23.066926575698503	26.738141650422353	25.86094866796621
24	17.41390513320338	26.18583495776478	23.716699155295647	32.68356075373619
25	23.106922326941827	24.374390640233994	23.78940526486838	28.7292817679558
26	15.894255874673629	25.718015665796344	30.221932114882506	28.16579634464752
27	18.71345029239766	18.323586744639375	31.773879142300192	31.189083820662766
28	14.921976592977892	20.64369310793238	34.03771131339402	30.39661898569571
29	14.271781534460338	19.733420026007803	35.98829648894669	30.006501950585175
30	13.495934959349592	20.0	39.05691056910569	27.447154471544717
31	13.76505043931012	19.980475105759844	44.516758867556135	21.737715587373902
32	16.87256176853056	15.149544863459038	43.62808842652796	24.349804941482446
33	14.19753086419753	16.146848602988953	45.02923976608187	24.626380766731643
34	11.241065627030538	19.200779727095515	48.37556855100715	21.182586094866796
35	13.027940220922677	18.25860948667966	47.33593242365172	21.377517868745937
36	11.306042884990253	17.64132553606238	46.00389863547758	25.048732943469787
37	10.822229444263893	18.297042573935652	47.18882027949301	23.691907702307443
38	11.825860948667966	18.421052631578945	46.393762183235864	23.359324236517217
39	12.085769980506821	17.93372319688109	46.198830409356724	23.781676413255358
40	8.349577647823262	22.839506172839506	45.71150097465887	23.099415204678362
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.5
15	1.0
16	4.5
17	8.0
18	8.0
19	11.0
20	14.0
21	20.5
22	27.0
23	27.0
24	37.5
25	48.0
26	58.5
27	69.0
28	69.0
29	72.0
30	75.0
31	80.0
32	85.0
33	85.0
34	105.0
35	125.0
36	156.0
37	187.0
38	187.0
39	192.0
40	197.0
41	208.5
42	220.0
43	220.0
44	221.5
45	223.0
46	223.0
47	223.0
48	223.0
49	211.0
50	199.0
51	178.0
52	157.0
53	157.0
54	159.0
55	161.0
56	155.0
57	149.0
58	149.0
59	166.0
60	183.0
61	184.0
62	185.0
63	185.0
64	169.0
65	153.0
66	135.5
67	118.0
68	118.0
69	107.0
70	96.0
71	84.0
72	72.0
73	72.0
74	63.5
75	55.0
76	42.0
77	29.0
78	29.0
79	19.0
80	9.0
81	5.0
82	1.0
83	1.0
84	2.0
85	3.0
86	1.5
87	0.0
88	0.0
89	0.0
90	0.0
91	3.0
92	6.0
93	6.0
94	3.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.16244314489928524
8	0.0649772579597141
9	0.03248862897985705
10	0.03248862897985705
11	0.0
12	0.03248862897985705
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.16244314489928524
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.03248862897985705
26	0.4548408057179987
27	0.0
28	0.0649772579597141
29	0.0649772579597141
30	0.09746588693957114
31	0.16244314489928524
32	0.0649772579597141
33	0.0
34	0.0
35	0.0
36	0.0
37	0.03248862897985705
38	0.0
39	0.0
40	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
40	3078.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	95.74398960363872
#Duplication Level	Percentage of deduplicated	Percentage of total
1	97.25144214455378	93.1124106562703
2	1.9341703427214116	3.7037037037037033
3	0.40719375636240246	1.1695906432748537
4	0.20359687818120123	0.7797270955165692
5	0.0678656260604004	0.3248862897985705
6	0.10179843909060061	0.5847953216374269
7	0.0	0.0
8	0.0	0.0
9	0.0	0.0
>10	0.0339328130302002	0.3248862897985705
>50	0.0	0.0
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TCTACCGGGGTCGACTTTTTTTTTTTTTTTTTTTTTTTTT	10	0.3248862897985705	No Hit
TCTACCGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCG	6	0.1949317738791423	No Hit
TCTACCGGGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTT	6	0.1949317738791423	No Hit
TCTACCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC	6	0.1949317738791423	No Hit
TCTACCGGGCTCGTCTTAATCAGCAACATCTTTTGATACA	5	0.16244314489928524	No Hit
TCTACCGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTT	5	0.16244314489928524	No Hit
TCTACCGGGATGAGATGGACTTAAATGAATTGACGGGTAC	4	0.1299545159194282	No Hit
TCTACCGGGAAGCAGTGGTATCAACGCCGAGTCCCCTTTT	4	0.1299545159194282	No Hit
TCTACCGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCC	4	0.1299545159194282	No Hit
TCTACCGGGGGGGTTGGGGATTTAGCTCCGTGGTAGAGCC	4	0.1299545159194282	No Hit
TCTACCGGGACAGCTTTACTCTTTCAGGTCACTCTGGGGC	4	0.1299545159194282	No Hit
TCTACCGGGAGAGTCGATTTTTTTTTTTTTTTTTTTTTTT	4	0.1299545159194282	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.03248862897985705	0.0	0.0	0.0	0.0
12	0.03248862897985705	0.0	0.0	0.0	0.0
13	0.03248862897985705	0.0	0.0	0.0	0.0
14	0.03248862897985705	0.0	0.0	0.0	0.0
15	0.03248862897985705	0.0	0.0	0.0	0.0
16	0.03248862897985705	0.0	0.0	0.0	0.0
17	0.03248862897985705	0.0	0.0	0.0	0.0
18	0.03248862897985705	0.0	0.0	0.0	0.0
19	0.03248862897985705	0.0	0.0	0.0	0.0
20	0.03248862897985705	0.0	0.0	0.0	0.0
21	0.03248862897985705	0.0	0.0	0.0	0.0
22	0.03248862897985705	0.0	0.0	0.0	0.0
23	0.03248862897985705	0.0	0.0	0.0	0.0
24	0.03248862897985705	0.0	0.0	0.0	0.0
25	0.03248862897985705	0.0	0.0	0.0	0.0
26	0.03248862897985705	0.0	0.0	0.0	0.0
27	0.03248862897985705	0.0	0.0	0.0	0.0
28	0.03248862897985705	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACCGGGT	40	5.1419192E-8	33.47541	4
ACCGGGG	60	0.0	33.47541	4
ACCGGGC	75	0.0	33.47541	4
ACCGGGA	90	0.0	33.47541	4
TACCGGG	265	0.0	33.47541	3
CGGGAAG	25	1.7469167E-4	33.47541	6
CCGGGGG	30	1.1763537E-5	33.47541	5
CCGGGCT	30	1.1763537E-5	33.47541	5
CCGGGCC	25	1.7469167E-4	33.47541	5
CCGGGCA	20	0.0025657502	33.47541	5
CCGGGAT	25	1.7469167E-4	33.47541	5
CCGGGAG	20	0.0025657502	33.47541	5
CCGGGAA	35	7.8278754E-7	33.47541	5
CTACCGG	300	0.0	33.47541	2
TCTACCG	310	0.0	32.935486	1
>>END_MODULE