FastQCFastQC Report
Fri 17 Jun 2016
SRR1526159_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526159_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8852
Sequences flagged as poor quality0
Sequence length40
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTACCGGGTACCTGGTTGATCCTGCCAGTAGCATATGCT1531.728422955264347No Hit
TCTACCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGC760.8585630366018979No Hit
TCTACCGGGTCACAGATCCTCTTCTGAGATGAGTTTTTGT630.7117035698147312No Hit
TCTACCGGGTCACAGATCCTCTTCTGAAGGAGATTGGGCG620.7004066877541798No Hit
TCTACCGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAG420.4744690465431541No Hit
TCTACCGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCA310.3502033438770899No Hit
TCTACCGGGTCAAAGATCCTCTTCTGAAGGAGATTGGGCG290.3276095797559873No Hit
TCTACCGGGTCAAAGATCCTCTTCTGAGATGAGTTTTTGT210.23723452327157704No Hit
TCTACCGGGTAACTGGTTGATCCTGCCAGTAGCATATGCT150.1694532309082693No Hit
TCTACCGGGATGCCACAACTAGATACATCAACATGATTTA150.1694532309082693No Hit
TCTACCGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGA140.15815634884771804No Hit
TCTACCGGGTCCCTGGTTGATCCTGCCAGTAGCATATGCT130.14685946678716674No Hit
TCTACCGGGACTAATCCTAGCCCTAGCCCTACACAAATAT120.13556258472661548No Hit
TCTACCGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAACG100.11296882060551287No Hit
TCTACCGGTCACAGATCCTCTTCTGAGATGAGTTTTTGTT90.1016719385449616No Hit
TCTACCGGGGTCGACTTTTTTTTTTTTTTTTTTTTTTTTT90.1016719385449616No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAACATG200.00242797234.58381729
TCAACAT200.00242797234.58381728
AGATGAG251.689285E-434.58381727
ATCAACA200.00242797234.58381727
GAGATGA200.00242797234.58381726
TACATCA200.002498416534.3850624
ACATCAA200.002498416534.3850625
CTGCCAG200.002498416534.3850623
ATACATC200.002498416534.3850623
AACATGA200.002498416534.3850630
CATGATT200.002570470334.18857232
ACATGAT200.002570470334.18857231
AGCATAT200.002570470334.18857231
ATGATTT200.002644160333.9943233
GATACAT200.002644160333.9943222
CCTGCCA251.871659E-433.9943222
TTCTGAA301.3254972E-533.9943222
GTTGATC200.002719513633.8022616
ACCGGGC503.5288394E-1033.802264
ACCGGGA2200.033.802264
Produced by FastQC (version 0.11.5)