##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1526159_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 8852 Sequences flagged as poor quality 0 Sequence length 40 %GC 49 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 20.70921825576141 29.0 4.0 33.0 4.0 34.0 2 13.12539539087212 4.0 4.0 23.0 4.0 30.0 3 12.081450519656574 4.0 4.0 20.0 4.0 31.0 4 10.73339358337099 4.0 4.0 15.0 4.0 28.0 5 12.045639403524627 4.0 4.0 21.0 4.0 30.0 6 11.344780840488026 4.0 4.0 20.0 4.0 29.0 7 12.231586082241302 4.0 4.0 23.0 4.0 31.0 8 12.315860822413015 4.0 4.0 24.0 4.0 31.0 9 11.861500225937641 4.0 4.0 23.0 4.0 31.0 10 11.015928603705378 4.0 4.0 19.0 4.0 30.0 11 11.480908269317668 4.0 4.0 21.0 4.0 33.0 12 10.85924084952553 4.0 4.0 18.0 4.0 31.0 13 10.235088115680073 4.0 4.0 15.0 4.0 30.0 14 10.44588793492996 4.0 4.0 15.0 4.0 31.0 15 9.915951197469498 4.0 4.0 11.0 4.0 31.0 16 8.563601446000904 4.0 4.0 4.0 4.0 26.0 17 8.155106190691368 4.0 4.0 4.0 4.0 24.0 18 7.876412110257569 4.0 4.0 4.0 4.0 23.0 19 7.748644374152734 4.0 4.0 4.0 4.0 23.0 20 7.973904202440126 4.0 4.0 4.0 4.0 25.0 21 7.368730230456394 4.0 4.0 4.0 4.0 22.0 22 7.210122006326254 4.0 4.0 4.0 4.0 20.0 23 7.320831450519656 4.0 4.0 4.0 4.0 21.0 24 7.0608901943063715 4.0 4.0 4.0 4.0 19.0 25 7.358111161319476 4.0 4.0 4.0 4.0 22.0 26 7.52643470402169 4.0 4.0 4.0 4.0 23.0 27 7.489493899683687 4.0 4.0 4.0 4.0 23.0 28 7.677586985991867 4.0 4.0 4.0 4.0 24.0 29 7.184365115228197 4.0 4.0 4.0 4.0 20.0 30 7.341730682331677 4.0 4.0 4.0 4.0 22.0 31 7.874152733845459 4.0 4.0 4.0 4.0 27.0 32 7.776208766380479 4.0 4.0 4.0 4.0 26.0 33 7.67950745594216 4.0 4.0 4.0 4.0 26.0 34 7.691030275643922 4.0 4.0 4.0 4.0 26.0 35 7.578400361500226 4.0 4.0 4.0 4.0 26.0 36 7.515815634884772 4.0 4.0 4.0 4.0 26.0 37 7.395390872119295 4.0 4.0 4.0 4.0 25.0 38 7.3389064618165385 4.0 4.0 4.0 4.0 25.0 39 7.208879349299593 4.0 4.0 4.0 4.0 23.0 40 7.1805241753276094 4.0 4.0 4.0 4.0 23.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1 1 7.026580049075665 1 2 2.844401166246376 1 3 1.8264519267083745 1 4 10.113658643009 1 5 3.4582048172535114 1 6 5.759228467028743 1 7 0.08897386551103992 1 8 0.8099897157122182 1 9 -4.3190460997638915 1 10 0.4763064702341868 1 11 -4.575378609788929 1 12 4.061964107864135 1 13 -1.022533649317829 1 14 2.260211043112033 1 15 -0.027197003504841888 1 16 0.16087856336780249 1 17 3.13976728812895 1 18 7.2036255082630705 1 19 0.5166480590550941 1 20 0.3080057596460932 1 21 1.2468221283011616 1 22 -1.1930971072731813 1 23 2.5933194129576167 1 24 2.9609515271546103 1 25 -1.4400278071921253 1 26 2.2183108936701705 1 27 -3.4127223823819204 1 28 -3.7218166925543956 1 29 -3.2387265082638157 1 30 -3.2750400737185794 1 31 -3.998795977920908 1 32 -3.8484511402067785 1 33 -3.7522987511325114 1 34 -3.601286341930863 1 35 -3.897935269904843 1 36 -3.80077486033033 1 37 -3.699980562741457 1 38 -3.6982362994641296 1 39 -3.6589925904183884 1 40 -3.446135887553771 2 1 4.026580049075665 2 2 7.244401166246375 2 3 -0.5735480732916258 2 4 5.513658643008998 2 5 6.8582048172535135 2 6 3.1592284670287434 2 7 10.088973865511038 2 8 7.0099897157122175 2 9 -6.719046099763892 2 10 2.0763064702341882 2 11 -1.175378609788929 2 12 6.461964107864135 2 13 4.17746635068217 2 14 3.8602110431120344 2 15 8.17280299649516 2 16 3.9608785633678014 2 17 11.739767288128949 2 18 5.003625508263071 2 19 5.916648059055094 2 20 4.908005759646094 2 21 5.846822128301161 2 22 2.0069028927268198 2 23 3.993319412957617 2 24 3.56095152715461 2 25 3.1599721928078743 2 26 4.418310893670172 2 27 5.187277617618079 2 28 1.878183307445604 2 29 0.7612734917361843 2 30 2.7249599262814206 2 31 5.801204022079093 2 32 4.951548859793222 2 33 1.647701248867489 2 34 7.398713658069137 2 35 5.902064730095158 2 36 4.9992251396696705 2 37 5.100019437258544 2 38 3.5017637005358697 2 39 6.341007409581612 2 40 5.9538641124462295 3 1 11.076580049075666 3 2 5.5444011662463755 3 3 -1.0235480732916251 3 4 9.013658643008998 3 5 10.758204817253512 3 6 1.259228467028743 3 7 -0.31102613448896044 3 8 2.609989715712219 3 9 4.530953900236108 3 10 -5.2236935297658125 3 11 -4.575378609788929 3 12 -1.0880358921358653 3 13 -3.4225336493178293 3 14 -4.439788956887966 3 15 -4.177197003504842 3 16 1.5108785633678021 3 17 1.0897672881289493 3 18 -2.146374491736929 3 19 1.4666480590550943 3 20 1.2580057596460934 3 21 -1.4031778716988388 3 22 1.206902892726819 3 23 -0.006680587042382946 3 24 0.7109515271546103 3 25 1.709972192807875 3 26 -0.3816891063298291 3 27 2.3372776176180796 3 28 1.2781833074456044 3 29 -0.2387265082638157 3 30 0.7249599262814206 3 31 0.25120402207909187 3 32 0.6515488597932215 3 33 -1.0022987511325114 3 34 1.3987136580691368 3 35 2.602064730095157 3 36 3.94922513966967 3 37 4.050019437258543 3 38 4.05176370053587 3 39 1.3410074095816116 3 40 3.553864112446229 4 1 1.4265800490756675 4 2 -5.835598833753624 4 3 -5.573548073291625 4 4 -5.606341356991002 4 5 -5.621795182746488 4 6 -4.280771532971257 4 7 -6.591026134488961 4 8 -5.270010284287781 4 9 -4.2790460997638915 4 10 -4.7636935297658125 4 11 -4.215378609788929 4 12 -3.6180358921358655 4 13 -3.9025336493178298 4 14 -4.859788956887966 4 15 -4.347197003504842 4 16 -4.239121436632198 4 17 -3.6602327118710507 4 18 -3.396374491736929 4 19 -3.2833519409449057 4 20 -3.4919942403539066 4 21 -3.153177871698839 4 22 -2.793097107273181 4 23 -3.006680587042383 4 24 -3.0390484728453897 4 25 -3.040027807192125 4 26 -3.381689106329829 4 27 -3.4127223823819204 4 28 -3.7218166925543956 4 29 -3.2387265082638157 4 30 -3.2750400737185794 4 31 -3.998795977920908 4 32 -3.8484511402067785 4 33 -3.7522987511325114 4 34 -3.601286341930863 4 35 -3.897935269904843 4 36 -3.80077486033033 4 37 -3.699980562741457 4 38 -3.6982362994641296 4 39 -3.6589925904183884 4 40 -3.446135887553771 5 1 12.226580049075665 5 2 12.244401166246375 5 3 0.026451926708375595 5 4 -2.586341356991001 5 5 1.1582048172535124 5 6 12.659228467028742 5 7 -0.8110261344889604 5 8 -0.4900102842877807 5 9 5.580953900236109 5 10 9.276306470234188 5 11 6.424621390211071 5 12 -0.4380358921358649 5 13 2.77746635068217 5 14 -0.43978895688796626 5 15 0.5728029964951578 5 16 -4.239121436632198 5 17 -3.6602327118710507 5 18 -3.396374491736929 5 19 -3.2833519409449057 5 20 -3.4919942403539066 5 21 -3.153177871698839 5 22 -2.793097107273181 5 23 -3.006680587042383 5 24 -3.0390484728453897 5 25 -3.040027807192125 5 26 -3.381689106329829 5 27 -3.4127223823819204 5 28 -3.7218166925543956 5 29 -3.2387265082638157 5 30 -3.2750400737185794 5 31 -3.998795977920908 5 32 -3.8484511402067785 5 33 -3.7522987511325114 5 34 -3.601286341930863 5 35 -3.897935269904843 5 36 -3.80077486033033 5 37 -3.699980562741457 5 38 -3.6982362994641296 5 39 -3.6589925904183884 5 40 -3.446135887553771 6 1 7.644761867257483 6 2 4.0444011662463755 6 3 5.408270108526558 6 4 5.150022279372635 6 5 -0.10543154638285124 6 6 12.122864830665106 6 7 3.2344284109655845 6 8 11.609989715712219 6 9 11.553681172963381 6 10 0.1853973793250958 6 11 6.879166844756524 6 12 16.29832774422777 6 13 16.577466350682172 6 14 17.060211043112034 6 15 17.936439360131523 6 16 14.124514927004165 6 17 14.43067637903804 6 18 6.6945345991721625 6 19 15.171193513600548 6 20 14.871642123282456 6 21 12.301367582846614 6 22 11.025084710908637 6 23 15.084228503866708 6 24 8.77913334533643 6 25 11.778154010989692 6 26 11.61831089367017 6 27 12.769095799436263 6 28 13.641819671081969 6 29 10.670364400827093 6 30 11.997687199008695 6 31 16.364840385715453 6 32 15.969730677975038 6 33 13.33861033977658 6 34 11.580531839887321 6 35 16.28388291191334 6 36 13.926497866942396 6 37 17.11820125544036 6 38 10.847218245990417 6 39 17.34100740958161 6 40 16.64477320335532 7 1 4.458158996444087 7 2 0.09703274519374361 7 3 3.5422414003925855 7 4 1.1452375903774197 7 5 -4.0575846564306985 7 6 -6.556561006655468 7 7 -8.100499818699486 7 8 -7.547905021129886 7 9 -6.666414520816524 7 10 -5.881588266607918 7 11 -5.89116808347314 7 12 -4.864351681609549 7 13 -3.8962178598441453 7 14 -4.623999483203756 7 15 -3.9535127929785263 7 16 -2.870700384000619 7 17 -2.1339169223973666 7 18 -2.0279534391053504 7 19 -2.5991414146291163 7 20 -2.123573187722328 7 21 -3.153177871698839 7 22 -2.793097107273181 7 23 -3.006680587042383 7 24 -3.0390484728453897 7 25 -3.040027807192125 7 26 -3.381689106329829 7 27 -3.4127223823819204 7 28 -3.7218166925543956 7 29 -3.2387265082638157 7 30 -3.2750400737185794 7 31 -3.998795977920908 7 32 -3.8484511402067785 7 33 -3.7522987511325114 7 34 -3.601286341930863 7 35 -3.897935269904843 7 36 -3.80077486033033 7 37 -3.699980562741457 7 38 -3.6982362994641296 7 39 -3.6589925904183884 7 40 -3.446135887553771 8 1 -5.548419950924334 8 2 -3.4139321670869585 8 3 -4.398548073291625 8 4 -0.8196746903243355 8 5 -7.033461849413155 8 6 -6.94910486630459 8 7 -7.6443594678222935 8 8 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2.751542135530226 9 10 3.6292476467047763 9 11 2.1305037431522464 9 12 4.897258225511193 9 13 0.9892310565645239 9 14 0.6484463372296805 9 15 1.8080971141422157 9 16 1.9961726810148601 9 17 -0.7778797706945797 9 18 1.4271549200277773 9 19 0.010765706113917695 9 20 1.2727116419990354 9 21 0.6115280106541023 9 22 0.7951381868444658 9 23 1.758025295310559 9 24 3.4903632918604925 9 25 3.548207486925522 9 26 4.088899128964289 9 27 1.5284540882063151 9 28 3.21935977803384 9 29 4.761273491736184 9 30 2.666136396869656 9 31 2.5894393161967386 9 32 4.445666506852046 9 33 4.424171837102782 9 34 1.81047836395149 9 35 3.9255941418598637 9 36 4.081578080846141 9 37 3.5941370843173672 9 38 4.654704877006459 9 39 3.8115956448757293 9 40 4.612687641857994 10 1 1.6265800490756668 10 2 -6.355598833753625 10 3 -2.773548073291625 10 4 4.513658643008998 10 5 7.958204817253511 10 6 7.459228467028742 10 7 5.788973865511041 10 8 6.609989715712219 10 9 9.38095390023611 10 10 8.276306470234188 10 11 11.724621390211071 10 12 2.4619641078641354 10 13 5.977466350682171 10 14 1.460211043112034 10 15 5.8728029964951585 10 16 3.8608785633678018 10 17 3.13976728812895 10 18 6.503625508263071 10 19 6.316648059055094 10 20 6.408005759646094 10 21 6.246822128301162 10 22 3.9069028927268183 10 23 5.193319412957616 10 24 4.46095152715461 10 25 5.359972192807875 10 26 6.918310893670172 10 27 9.38727761761808 10 28 8.778183307445605 10 29 5.061273491736185 10 30 7.124959926281421 10 31 8.101204022079093 10 32 6.6515488597932215 10 33 8.24770124886749 10 34 8.598713658069137 10 35 8.20206473009516 10 36 10.399225139669669 10 37 10.600019437258544 10 38 8.401763700535872 10 39 9.141007409581611 10 40 11.05386411244623 11 1 10.426580049075667 11 2 14.744401166246375 11 3 11.526451926708376 11 4 8.113658643009 11 5 12.458204817253511 11 6 12.459228467028742 11 7 12.388973865511039 11 8 3.609989715712219 11 9 5.980953900236107 11 10 11.176306470234186 11 11 8.92462139021107 11 12 8.661964107864135 11 13 4.17746635068217 11 14 6.960211043112032 11 15 6.272802996495157 11 16 2.8608785633678018 11 17 2.3397672881289493 11 18 1.7036255082630705 11 19 2.9166480590550936 11 20 2.2080057596460927 11 21 1.9468221283011609 11 22 0.9069028927268192 11 23 2.493319412957617 11 24 0.06095152715460994 11 25 0.6599721928078752 11 26 2.4183108936701716 11 27 1.1872776176180793 11 28 2.7781833074456044 11 29 1.4612734917361836 11 30 2.624959926281421 11 31 3.6012040220790915 11 32 1.2515488597932212 11 33 1.0477012488674893 11 34 4.1987136580691375 11 35 0.7020647300951568 11 36 1.2992251396696695 11 37 0.6000194372585437 11 38 1.3017637005358704 11 39 1.1410074095816123 11 40 2.553864112446229 12 1 9.326580049075666 12 2 11.544401166246375 12 3 -0.7735480732916251 12 4 10.513658643008998 12 5 -4.241795182746488 12 6 18.25922846702874 12 7 17.688973865511038 12 8 17.609989715712217 12 9 16.280953900236106 12 10 19.27630647023419 12 11 15.42462139021107 12 12 25.161964107864137 12 13 19.577466350682172 12 14 20.060211043112034 12 15 17.072802996495156 12 16 11.260878563367802 12 17 11.33976728812895 12 18 11.103625508263072 12 19 17.716648059055096 12 20 18.008005759646093 12 21 17.846822128301163 12 22 12.70690289272682 12 23 19.993319412957618 12 24 19.46095152715461 12 25 16.959972192807875 12 26 15.61831089367017 12 27 22.587277617618078 12 28 20.278183307445605 12 29 14.261273491736183 12 30 22.72495992628142 12 31 21.501204022079094 12 32 20.151548859793223 12 33 20.24770124886749 12 34 23.398713658069138 12 35 25.102064730095158 12 36 23.69922513966967 12 37 10.300019437258543 12 38 6.80176370053587 12 39 10.34100740958161 12 40 4.053864112446229 14 1 5.921818144313761 14 2 3.4729725948178025 14 3 3.13121383147028 14 4 -2.4863413569910016 14 5 1.5439191029677986 14 6 2.759228467028743 14 7 0.022307198844373488 14 8 2.086180191902695 14 9 1.0428586621408709 14 10 3.657258851186569 14 11 1.3770023425920233 14 12 2.328630774530801 14 13 2.5774663506821707 14 14 2.3935443764453677 14 15 -2.3319589082667473 14 16 -1.477216674727436 14 17 -0.9935660452043837 14 18 -0.6820887774512148 14 19 -1.2357328933258582 14 20 -1.9681847165443829 14 21 -1.2484159669369337 14 22 -1.2692875834636572 14 23 -2.482871063232859 14 24 -2.4676199014168185 14 25 -1.6590754262397436 14 26 -1.381689106329829 14 27 -1.4603414300009678 14 28 -1.8170547877924905 14 29 -1.6672979368352445 14 30 -0.6083734070519125 14 31 -0.6178435969685268 14 32 -0.5151178068734454 14 33 -0.5142035130372733 14 34 -0.8393815800261013 14 35 0.19730282533325205 14 36 -0.9436320031874734 14 37 -0.5571234198843138 14 38 0.3970017957739653 14 39 0.24576931434351668 14 40 0.45862601720813423 15 1 8.726580049075665 15 2 10.344401166246376 15 3 9.626451926708373 15 4 6.613658643009 15 5 -0.8417951827464876 15 6 17.75922846702874 15 7 17.38897386551104 15 8 15.609989715712219 15 9 17.180953900236105 15 10 14.276306470234188 15 11 11.92462139021107 15 12 12.561964107864133 15 13 9.27746635068217 15 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-3.006680587042383 119 24 -3.0390484728453897 119 25 -3.040027807192125 119 26 -3.381689106329829 119 27 -3.4127223823819204 119 28 -3.7218166925543956 119 29 -3.2387265082638157 119 30 -3.2750400737185794 119 31 -3.998795977920908 119 32 -3.8484511402067785 119 33 -3.7522987511325114 119 34 -3.601286341930863 119 35 -3.897935269904843 119 36 -3.80077486033033 119 37 -3.699980562741457 119 38 -3.6982362994641296 119 39 -3.6589925904183884 119 40 -3.446135887553771 120 1 0.8265800490756661 120 2 2.9194011662463755 120 3 -5.273548073291625 120 4 -4.611341356991002 120 5 0.7582048172535121 120 6 2.634228467028743 120 7 -2.1860261344889604 120 8 -1.515010284287781 120 9 -3.469046099763892 120 10 -2.3486935297658125 120 11 -0.4503786097889293 120 12 -1.7130358921358653 120 13 -2.4225336493178293 120 14 0.43521104311203374 120 15 0.6978029964951578 120 16 -1.8641214366321979 120 17 -1.6602327118710507 120 18 -2.521374491736929 120 19 -2.4083519409449057 120 20 -2.7419942403539066 120 21 -1.1531778716988388 120 22 -0.16809710727318095 120 23 -1.131680587042383 120 24 -1.0390484728453897 120 25 -1.790027807192125 120 26 -0.5066891063298291 120 27 -0.6627223823819204 120 28 -0.4718166925543956 120 29 0.5112734917361843 120 30 -0.025040073718579414 120 31 -3.998795977920908 120 32 -3.8484511402067785 120 33 -3.7522987511325114 120 34 -3.601286341930863 120 35 -3.897935269904843 120 36 -3.80077486033033 120 37 -3.699980562741457 120 38 -3.6982362994641296 120 39 -3.6589925904183884 120 40 -3.446135887553771 >>END_MODULE >>Per sequence quality scores fail #Quality Count 4 4725.0 5 278.0 6 267.0 7 232.0 8 163.0 9 292.0 10 345.0 11 328.0 12 215.0 13 159.0 14 118.0 15 69.0 16 63.0 17 61.0 18 57.0 19 92.0 20 148.0 21 213.0 22 249.0 23 241.0 24 168.0 25 126.0 26 108.0 27 57.0 28 37.0 29 20.0 30 9.0 31 5.0 32 5.0 33 2.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 0.0 0.0 100.0 0.0 2 0.0 0.0 0.0 100.0 3 0.0 0.0 100.0 0.0 4 0.0 100.0 0.0 0.0 5 0.0 0.0 0.0 100.0 6 0.0 0.0 0.0 100.0 7 98.18037974683544 0.46338155515370705 0.2147377938517179 1.1415009041591322 8 97.03653432869585 1.549598461712476 0.48637032009953624 0.9274968894921388 9 95.50904977375566 1.9004524886877827 1.6063348416289591 0.9841628959276018 10 46.299016838060794 27.14431009153577 15.052548310543564 11.504124759859872 11 32.692959656458356 24.409537800881456 24.251327833653523 18.64617470900667 12 29.62042476276548 22.53727971079982 24.310890194306374 23.531405332128333 13 22.00881256355214 22.00881256355214 27.793469664444697 28.188905208451022 14 20.736806418804385 28.82811617131879 31.72109842920104 18.713978980675783 15 30.908269317668324 25.417984636240398 27.89200180750113 15.781744238590148 16 31.348847718029827 27.428829643018528 25.293718933574333 15.928603705377316 17 26.813559322033896 23.977401129943505 34.33898305084746 14.87005649717514 18 25.35306745000565 25.296576658004742 29.657665800474525 19.692690091515082 19 24.82470029405112 28.093191585614115 25.593757068536533 21.488351051798237 20 23.655671034794395 30.08359692724808 31.03253502033439 15.228197017623135 21 21.65612291007682 26.016719385449615 31.732941708088568 20.594215996385 22 21.159060099412564 23.486217803886127 32.87392679620425 22.480795300497064 23 21.941023613151057 25.861484578013783 31.781719579708508 20.415772229126652 24 21.531857207410752 26.186172616357883 29.676909173068232 22.605061003163126 25 25.186524983043185 26.31697942572914 27.390911146280807 21.105584444946867 26 24.949060448268053 26.443287299071766 25.164138555580713 23.443513697079467 27 19.2638731596829 30.11325028312571 25.164212910532274 25.45866364665912 28 22.057659694742792 33.06953080836631 24.16054267947993 20.712266817410967 29 28.74461817357806 28.43870382959438 23.226829820983458 19.589848175844097 30 24.72875226039783 26.67269439421338 31.001356238698012 17.597197106690775 31 27.11864406779661 29.570621468926557 25.254237288135595 18.056497175141242 32 30.430839002267575 25.26077097505669 25.952380952380956 18.356009070294785 33 26.50248531405332 27.123813827383643 26.22006326253954 20.153637596023497 34 20.890294881934246 28.573042594057167 33.10360411252965 17.43305841147893 35 19.88251242657027 28.29868956168098 35.12200632625395 16.6967916854948 36 21.351870690629593 32.4064654685204 28.077314343845373 18.164349497004633 37 23.04824313636877 28.505253643656086 31.521861936504347 16.924641283470795 38 26.163578852236785 28.524627202892 27.90329868956168 17.408495255309532 39 23.206417353971304 27.6126991300418 25.03671901480059 24.144164501186307 40 21.000903750564845 26.355625847266158 31.439222774514235 21.20424762765477 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 1.0 10 2.0 11 3.0 12 4.0 13 4.0 14 13.5 15 23.0 16 25.0 17 27.0 18 27.0 19 40.0 20 53.0 21 59.5 22 66.0 23 66.0 24 70.0 25 74.0 26 88.5 27 103.0 28 103.0 29 118.0 30 133.0 31 170.0 32 207.0 33 207.0 34 231.0 35 255.0 36 294.5 37 334.0 38 334.0 39 399.5 40 465.0 41 536.0 42 607.0 43 607.0 44 657.5 45 708.0 46 708.5 47 709.0 48 709.0 49 750.0 50 791.0 51 869.5 52 948.0 53 948.0 54 894.5 55 841.0 56 795.5 57 750.0 58 750.0 59 730.0 60 710.0 61 623.5 62 537.0 63 537.0 64 399.5 65 262.0 66 195.5 67 129.0 68 129.0 69 96.5 70 64.0 71 42.5 72 21.0 73 21.0 74 20.5 75 20.0 76 12.5 77 5.0 78 5.0 79 3.5 80 2.0 81 1.5 82 1.0 83 1.0 84 1.0 85 1.0 86 0.5 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.045187528242205156 8 0.12426570266606417 9 0.13556258472661548 10 0.03389064618165387 11 0.03389064618165387 12 0.0 13 0.011296882060551289 14 0.03389064618165387 15 0.0 16 0.0 17 0.022593764121102578 18 0.011296882060551289 19 0.11296882060551287 20 0.0 21 0.0 22 0.0 23 0.011296882060551289 24 0.0 25 0.06778129236330774 26 0.2033438770899232 27 0.24853140533212834 28 0.07907817442385902 29 0.2937189335743335 30 0.045187528242205156 31 0.022593764121102578 32 0.36150022593764125 33 0.0 34 0.011296882060551289 35 0.0 36 0.05648441030275644 37 0.011296882060551289 38 0.0 39 0.011296882060551289 40 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 40 8852.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 86.57930411206507 #Duplication Level Percentage of deduplicated Percentage of total 1 94.55897703549061 81.86850429281517 2 3.770876826722338 6.529597830998644 3 0.75678496868476 1.9656574785359242 4 0.32620041753653445 1.1296882060551288 5 0.11743215031315239 0.508359692724808 6 0.11743215031315239 0.6100316312697696 7 0.10438413361169101 0.6326253953908721 8 0.03914405010438413 0.27112516945323095 9 0.026096033402922752 0.2033438770899232 >10 0.1304801670146138 2.2819701762313604 >50 0.03914405010438413 2.270673294170809 >100 0.013048016701461376 1.728422955264347 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source TCTACCGGGTACCTGGTTGATCCTGCCAGTAGCATATGCT 153 1.728422955264347 No Hit TCTACCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGC 76 0.8585630366018979 No Hit TCTACCGGGTCACAGATCCTCTTCTGAGATGAGTTTTTGT 63 0.7117035698147312 No Hit TCTACCGGGTCACAGATCCTCTTCTGAAGGAGATTGGGCG 62 0.7004066877541798 No Hit TCTACCGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAG 42 0.4744690465431541 No Hit TCTACCGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCA 31 0.3502033438770899 No Hit TCTACCGGGTCAAAGATCCTCTTCTGAAGGAGATTGGGCG 29 0.3276095797559873 No Hit TCTACCGGGTCAAAGATCCTCTTCTGAGATGAGTTTTTGT 21 0.23723452327157704 No Hit TCTACCGGGTAACTGGTTGATCCTGCCAGTAGCATATGCT 15 0.1694532309082693 No Hit TCTACCGGGATGCCACAACTAGATACATCAACATGATTTA 15 0.1694532309082693 No Hit TCTACCGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGA 14 0.15815634884771804 No Hit TCTACCGGGTCCCTGGTTGATCCTGCCAGTAGCATATGCT 13 0.14685946678716674 No Hit TCTACCGGGACTAATCCTAGCCCTAGCCCTACACAAATAT 12 0.13556258472661548 No Hit TCTACCGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAACG 10 0.11296882060551287 No Hit TCTACCGGTCACAGATCCTCTTCTGAGATGAGTTTTTGTT 9 0.1016719385449616 No Hit TCTACCGGGGTCGACTTTTTTTTTTTTTTTTTTTTTTTTT 9 0.1016719385449616 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.011296882060551287 0.0 0.0 0.0 0.0 9 0.011296882060551287 0.0 0.0 0.0 0.0 10 0.011296882060551287 0.0 0.0 0.0 0.0 11 0.011296882060551287 0.0 0.0 0.0 0.0 12 0.011296882060551287 0.0 0.0 0.0 0.0 13 0.022593764121102575 0.0 0.0 0.0 0.0 14 0.03389064618165386 0.0 0.0 0.0 0.0 15 0.03389064618165386 0.0 0.0 0.0 0.0 16 0.03389064618165386 0.0 0.0 0.0 0.0 17 0.03389064618165386 0.0 0.0 0.0 0.0 18 0.03389064618165386 0.0 0.0 0.0 0.0 19 0.03389064618165386 0.0 0.0 0.0 0.0 20 0.03389064618165386 0.0 0.0 0.0 0.0 21 0.03389064618165386 0.0 0.0 0.0 0.0 22 0.03389064618165386 0.0 0.0 0.0 0.0 23 0.03389064618165386 0.0 0.0 0.0 0.0 24 0.03389064618165386 0.0 0.0 0.0 0.0 25 0.03389064618165386 0.0 0.0 0.0 0.0 26 0.03389064618165386 0.0 0.0 0.0 0.0 27 0.04518752824220515 0.0 0.0 0.0 0.0 28 0.04518752824220515 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CAACATG 20 0.002427972 34.583817 29 TCAACAT 20 0.002427972 34.583817 28 AGATGAG 25 1.689285E-4 34.583817 27 ATCAACA 20 0.002427972 34.583817 27 GAGATGA 20 0.002427972 34.583817 26 TACATCA 20 0.0024984165 34.38506 24 ACATCAA 20 0.0024984165 34.38506 25 CTGCCAG 20 0.0024984165 34.38506 23 ATACATC 20 0.0024984165 34.38506 23 AACATGA 20 0.0024984165 34.38506 30 CATGATT 20 0.0025704703 34.188572 32 ACATGAT 20 0.0025704703 34.188572 31 AGCATAT 20 0.0025704703 34.188572 31 ATGATTT 20 0.0026441603 33.99432 33 GATACAT 20 0.0026441603 33.99432 22 CCTGCCA 25 1.871659E-4 33.99432 22 TTCTGAA 30 1.3254972E-5 33.99432 22 GTTGATC 20 0.0027195136 33.80226 16 ACCGGGC 50 3.5288394E-10 33.80226 4 ACCGGGA 220 0.0 33.80226 4 >>END_MODULE