FastQCFastQC Report
Fri 17 Jun 2016
SRR1526158_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526158_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences132526
Sequences flagged as poor quality0
Sequence length56
%GC51

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATGGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAG46173.483844679534582No Hit
GTAATGGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTCGTCTCAAAGATTAAG11210.8458717534672441No Hit
GTAATGGGGTCACAGATCCTCTTCTGAGATGAGTTTTTGTTCAATCCGCCCAATCT7120.5372530673226386No Hit
GTAATGGGGTACCTGGTTGATCCTGCCAGTAGCATATGCCTGTCTCAAAGATTAAG5790.43689540165703333No Hit
GTAATGGGGTCACAGATCCTCTTCTGAAGGAGATTGGGCGGATTGAACAAAAACTC5680.4285951435944645No Hit
GTAATGGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCCAAGATTAAG5530.41727660987277965No Hit
GTAATGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4360.3289920468436382No Hit
GTAATGGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCCACGATTAAG3690.2784359295534461No Hit
GTAATGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC3250.2452348973031707No Hit
GTAATGGGGGGGCGCTGACCCCCCTTCCCGGGGGGGGATGCGTGCATTTATCAGAT2530.19090593543908366No Hit
GTAATGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACAAAAAAAAAAAAAAA2310.17430541931394594No Hit
GTAATGGGGTACCTGGTTGATCCTGCCAGTAGCATATGCCGTCTTCTGCTTGGATC2270.17128714365483No Hit
GTAATGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTC2220.16751429908093507No Hit
GTAATGGGGTCACAGATCCTCTTCTGAAGGAGATTGGGCGCATTGAACAAAAACTC2180.1644960234218191No Hit
GTAATGGGGCTCCTACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCGAC1880.1418589559784495No Hit
GTAATGGGGTCACAGATCCTCTTCTGAGATGAGTTTTTGTCCAATCCGCCCAATCT1700.12827671551242775No Hit
GTAATGGGGGGTTCCTTTGGTCGCTCGCTCCTCTCCTACTTGGATAACTGTGGTAA1470.11092163047251105No Hit
GTAATGGGGATGCCACAACTAGATACATCAACATGATTTATCACAATTATCTCATC1470.11092163047251105No Hit
GTAATGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTTCTTCC1460.11016706155773205No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATTGA150.00813920950.42246240
ACCTATG150.00813920950.42246240
CTCGGAT150.00813920950.42246239
CACGATT355.1775714E-850.0781547
CTTAGGT150.00836277150.07814814
TAGATCC150.00837532350.05916213
ACGCTTA150.00840047150.0212211
GATTACG150.00840047150.0212250
TGATGTC150.00840047150.0212212
GTCATAG150.00843829749.964429
GCAAAAC204.1850258E-449.964429
GTACGCT150.00843829749.964429
GACGCAC252.0861258E-549.9644179
GTCATTA150.00848892949.8888929
CTACTGA150.00848892949.8888931
GATAATG150.00851432949.8512128
TTGCGGT150.00851432949.8512127
ATGGGGG64700.049.4671254
TGGGGTA11650.049.3865
AATGGGG126650.049.125283
Produced by FastQC (version 0.11.5)