##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1526158_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 132526 Sequences flagged as poor quality 0 Sequence length 56 %GC 51 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 35.8062493397522 38.0 35.0 39.0 29.0 40.0 2 35.26707966738602 38.0 35.0 39.0 26.0 40.0 3 34.61257413639588 38.0 35.0 39.0 20.0 40.0 4 33.43157569080784 38.0 33.0 39.0 15.0 40.0 5 35.60257609827506 38.0 35.0 39.0 27.0 40.0 6 35.53589484327603 38.0 35.0 39.0 26.0 40.0 7 35.89698625175437 38.0 35.0 39.0 29.0 40.0 8 35.6109518132291 38.0 35.0 39.0 27.0 40.0 9 35.597837405490246 38.0 35.0 39.0 27.0 40.0 10 34.743627665514694 38.0 35.0 39.0 24.0 40.0 11 34.55109186121968 38.0 33.0 39.0 25.0 40.0 12 33.731607382702265 38.0 32.0 39.0 21.0 40.0 13 33.331323664790304 38.0 32.0 39.0 19.0 40.0 14 33.94612377948478 38.0 33.0 39.0 22.0 40.0 15 32.78339344732355 38.0 31.0 39.0 17.0 40.0 16 32.29955631347811 36.0 29.0 39.0 18.0 40.0 17 31.293572581983913 36.0 27.0 39.0 15.0 40.0 18 30.693765751626096 35.0 26.0 38.0 13.0 40.0 19 31.79282555875828 36.0 28.0 38.0 17.0 40.0 20 32.511756183692256 36.0 30.0 39.0 19.0 40.0 21 32.83222914748804 38.0 30.0 39.0 18.0 40.0 22 32.121840242669364 37.0 29.0 39.0 16.0 40.0 23 32.87108944659916 38.0 31.0 39.0 18.0 40.0 24 32.206397235259494 37.0 29.0 39.0 16.0 40.0 25 32.655056366297934 37.0 30.0 39.0 18.0 40.0 26 33.13457736595083 38.0 32.0 39.0 18.0 40.0 27 33.37535276096766 38.0 33.0 39.0 20.0 40.0 28 33.265185699409926 38.0 32.0 39.0 20.0 40.0 29 32.83437966889516 38.0 31.0 39.0 17.0 40.0 30 32.48042648235063 37.0 31.0 39.0 17.0 40.0 31 33.159387591868764 38.0 33.0 39.0 18.0 40.0 32 33.09249505757361 38.0 33.0 39.0 18.0 40.0 33 32.89333413820684 37.0 32.0 39.0 17.0 40.0 34 32.69702548933794 37.0 32.0 39.0 17.0 40.0 35 32.61836922566138 37.0 32.0 39.0 16.0 40.0 36 31.88846716870652 36.0 31.0 38.0 10.0 40.0 37 31.62150068665771 36.0 30.0 38.0 4.0 40.0 38 30.914348882483438 35.0 29.0 38.0 4.0 40.0 39 29.849191856692272 35.0 27.0 38.0 4.0 40.0 40 27.900042255859226 33.0 19.0 38.0 4.0 39.0 41 25.92775002640991 31.0 15.0 37.0 4.0 39.0 42 26.841623530477037 32.0 19.0 37.0 4.0 39.0 43 26.953526100538763 32.0 19.0 37.0 4.0 39.0 44 25.957510224408797 31.0 16.0 37.0 4.0 39.0 45 26.829520245083984 32.0 19.0 37.0 4.0 39.0 46 24.896488236270617 29.0 14.0 36.0 4.0 39.0 47 24.063549794002686 29.0 10.0 36.0 4.0 39.0 48 24.700639874439734 29.0 12.0 36.0 4.0 39.0 49 23.756410062931046 28.0 7.0 35.0 4.0 39.0 50 24.303774353711724 29.0 6.0 36.0 4.0 39.0 51 24.57463441136079 30.0 4.0 36.0 4.0 39.0 52 24.440985165175135 30.0 4.0 36.0 4.0 39.0 53 24.24441996287521 30.0 4.0 36.0 4.0 39.0 54 23.921124911338154 30.0 4.0 36.0 4.0 39.0 55 23.56046360714124 30.0 4.0 36.0 4.0 39.0 56 22.607503433288564 28.0 4.0 35.0 4.0 38.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1 1 4.773833049154703 1 2 4.8948270667313025 1 3 5.9421096695458075 1 4 7.279152015848094 1 5 4.502831779325298 1 6 4.588403069483341 1 7 4.392938574588854 1 8 4.678629167376862 1 9 4.632306386189029 1 10 5.2493427218942585 1 11 -1.1116092531402657 1 12 -9.062624509671299 1 13 -18.829642867997954 1 14 -7.480802080850371 1 15 0.781747186925358 1 16 -1.967007525999616 1 17 -6.628587982671689 1 18 -5.4766255598269815 1 19 -1.0116200238303215 1 20 -1.5528146891949781 1 21 5.798090716352032 1 22 6.755844740987982 1 23 5.832124504497713 1 24 6.463619270132845 1 25 5.551641869730837 1 26 5.755036751979574 1 27 5.475068807188272 1 28 5.436286213706072 1 29 5.654928876224552 1 30 6.04145628819947 1 31 4.958324686930201 1 32 4.276310252587276 1 33 4.694482902663896 1 34 4.250707517681349 1 35 4.16695470386539 1 36 2.5488199826570828 1 37 2.6409772849011155 1 38 3.5375109029847387 1 39 2.5468717358742197 1 40 -11.570692617827302 1 41 -3.180778522199134 1 42 -4.589664126172838 1 43 -10.826572708635064 1 44 -15.469333113133759 1 45 -4.890696952711529 1 46 -7.293553179283089 1 47 -12.01176480230493 1 48 -7.417212405318823 1 49 -14.267610742420338 1 50 -5.683775545417582 1 51 -0.13980865346972138 1 52 -0.4311170455582456 1 53 0.0242992663472279 1 54 0.07639620051891782 1 55 0.13177772703426882 1 56 3.3492240999650846 2 1 4.9728692641079775 2 2 5.076972705360582 2 3 6.219854996648614 2 4 7.790590878776438 2 5 4.798733259076073 2 6 4.668815842069016 2 7 4.448477904806921 2 8 4.7271104757880735 2 9 4.638342211734198 2 10 4.84158376550797 2 11 3.2741968216260915 2 12 -1.2882865034407693 2 13 0.7627928796655965 2 14 4.284287483012562 2 15 4.991151392532835 2 16 4.1246494054645595 2 17 6.481614509540147 2 18 4.564408318677689 2 19 5.794552094549736 2 20 4.753649485259849 2 21 6.463053722582561 2 22 7.418373946595459 2 23 6.528288834715781 2 24 7.301236092562753 2 25 6.277206511475384 2 26 6.092507546372097 2 27 5.587023635848709 2 28 5.711159656073672 2 29 5.945378642579691 2 30 5.954472253931556 2 31 4.551198518705899 2 32 5.1689796451106425 2 33 4.9172730116981676 2 34 4.609693109581656 2 35 4.69504076305542 2 36 5.414668892314399 2 37 5.3279905247765065 2 38 5.526510124168542 2 39 5.867159897867989 2 40 3.1982910270325107 2 41 7.503508860978435 2 42 6.21826032865582 2 43 5.561764518779267 2 44 -4.814786773569896 2 45 5.804104449157631 2 46 4.779120107321273 2 47 2.1028185310284044 2 48 4.904243980974012 2 49 -1.8526477361898088 2 50 4.731187460812947 2 51 7.470783246841805 2 52 6.714326879675401 2 53 7.326674655755326 2 54 6.976951107060973 2 55 6.776880920180684 2 56 7.830045751055426 3 1 5.042962722051897 3 2 5.175103546482077 3 3 6.023593314405623 3 4 7.64105816849607 3 5 4.878172511412522 3 6 4.818348552349384 3 7 4.4858610823770135 3 8 4.829914214105834 3 9 4.722454361266905 3 10 5.215415541208905 3 11 4.489150092654128 3 12 4.379937795624649 3 13 5.230082599291762 3 14 5.134754772732187 3 15 -0.8826803831681005 3 16 6.6246494054645595 3 17 7.5189976871102395 3 18 7.872819533630963 3 19 7.088944617914223 3 20 6.361126120773868 3 21 7.313521012302193 3 22 7.497813198931908 3 23 7.046980423500827 3 24 7.899366933684249 3 25 7.179075670353889 3 26 5.8915729669328485 3 27 5.960855411549645 3 28 5.7952718056063794 3 29 6.618275838841377 3 30 6.2535376744923 3 31 4.882974219640484 3 32 5.337203944176064 3 33 5.094843105156112 3 34 4.698478156310628 3 35 5.358592164924577 3 36 5.5314913222209405 3 37 6.028925104215762 3 38 6.1293138624862955 3 39 7.469963636185749 3 40 8.721655513013818 3 41 10.844630356305537 3 42 9.582746309964229 3 43 9.851484144947491 3 44 9.937549675028237 3 45 9.262048374391277 3 46 10.63893319143342 3 47 11.500014792710644 3 48 10.801440242656255 3 49 11.166043852595234 3 50 10.17978559165407 3 51 9.737138387028725 3 52 9.756382954441754 3 53 9.910786805288037 3 54 10.360128677154432 3 55 9.884357555694702 3 56 10.50294294731711 4 1 5.038876653805865 4 2 4.96280187293285 4 3 6.109987576522549 4 4 7.582809961584527 4 5 4.353873445991965 4 6 4.634236402816676 4 7 4.259823264511333 4 8 4.576012888307098 4 9 4.613217238902202 4 10 4.652516171506662 4 11 4.711210514301598 4 12 5.247162312034128 4 13 5.695720504095071 4 14 5.4958936555837425 4 15 5.586543698553264 4 16 7.109131039891857 4 17 8.475094187870948 4 18 8.783621533196271 4 19 7.509940053689057 4 20 6.94389073716161 4 21 6.751990910150486 4 22 7.441673229360077 4 23 6.360564426978328 4 24 6.794433223621219 4 25 6.391225203064174 4 26 5.050603612444689 4 27 5.466832373079747 4 28 5.023374392000644 4 29 5.947443411108274 4 30 5.609503768819621 4 31 4.78942933809299 4 32 4.427836415377971 4 33 4.14576681739258 4 34 4.486632905278249 4 35 4.567851021694846 4 36 4.092521533044678 4 37 4.914862943815844 4 38 4.855873306085517 4 39 5.628363983936236 4 40 -0.48508215271102273 4 41 9.280122640591564 4 42 8.117651765300032 4 43 7.905646283612995 4 44 8.84624343725384 4 45 7.6731111868233555 4 46 10.177207479631637 4 47 10.484213879865614 4 48 9.818349610185052 4 49 10.050242939750202 4 50 9.39221767163668 4 51 9.11755568761555 4 52 8.6633596986278 4 53 9.161993064796839 4 54 8.50800957261194 4 55 8.775794199902485 4 56 8.84258650306586 5 1 5.164458049154703 5 2 5.250050496682988 5 3 6.303673679207641 5 4 7.895471460292537 5 5 4.583825136813218 5 6 4.896072151608948 5 7 4.728309951400448 5 8 5.028568780903434 5 9 4.9466179803919275 5 10 5.245191151845951 5 11 5.681416109178578 5 12 5.929827181149953 5 13 6.037884305915092 5 14 5.982513136540938 5 15 7.02465177629734 5 16 7.623949599604245 5 17 8.925155978680966 5 18 9.540660068192341 5 19 8.086055096942623 5 20 7.631364054766372 5 21 7.57005629606218 5 22 8.079893654031459 5 23 7.235657113193362 5 24 7.546348738731879 5 25 7.641466748957889 5 26 5.962388805119673 5 27 5.900491512502285 5 28 6.291434160565974 5 29 6.229883586369482 5 30 6.303156167426522 5 31 5.002708744901216 5 32 4.765214238094526 5 33 4.9564847142580994 5 34 4.446435174686179 5 35 4.721227046860559 5 36 5.979073605845489 5 37 5.998314241422857 5 38 6.9579517242407825 5 39 7.487391059545718 5 40 8.8962457879698 5 41 11.340205777317777 5 42 10.485969931798174 5 43 10.496343958031602 5 44 10.726998408605368 5 45 10.505287346805382 5 46 11.392059743422227 5 47 11.242915149385894 5 48 11.156761024632864 5 49 11.824403146468551 5 50 11.186016121249086 5 51 10.082413568752504 5 52 10.3264312636205 5 53 9.81868332431824 5 54 10.193168543514087 5 55 9.577052485488373 5 56 9.676065887404697 6 1 4.674261970723329 6 2 4.8341653020254185 6 3 5.941496924447769 6 4 7.357889760946133 6 5 4.742108740109607 6 6 4.30776581458138 6 7 4.28969102556924 6 8 4.290148775219997 6 9 4.280590699914519 6 10 4.878325565031517 6 11 5.016454472349928 6 12 5.490071568760072 6 13 5.6902713476883235 6 14 5.059026350522181 6 15 6.897556010454771 6 16 7.4254557092945035 6 17 7.952294370269488 6 18 8.989367087231845 6 19 7.975512329110856 6 20 6.974146095118748 6 21 6.996313755567723 6 22 7.221837388046808 6 23 6.859698033909474 6 24 7.490580054446568 6 25 6.747107556005346 6 26 5.941004889234478 6 27 5.138059003266704 6 28 5.253994547039401 6 29 5.747147013479456 6 30 5.86958128819947 6 31 5.319844294773336 6 32 5.230047997685315 6 33 5.136578490899193 6 34 4.896847223563697 6 35 5.285520880335977 6 36 4.952006257166882 6 37 6.137913559410922 6 38 5.96214325592592 6 39 6.344665853521281 6 40 4.885802480211915 6 41 10.161745987604789 6 42 9.38153685421932 6 43 9.550265526659054 6 44 10.100826200591733 6 45 9.032709910033567 6 46 10.970846330520832 6 47 11.76458323691076 6 48 10.525189555465488 6 49 11.371788767383581 6 50 10.568369062425557 6 51 10.669014875942043 6 52 9.996579032873129 6 53 10.361309070268792 6 54 10.982952573067937 6 55 10.244216452524462 6 56 9.810621158788614 7 1 4.814458049154702 7 2 5.035243733397969 7 3 6.03335966954581 7 4 7.497693682514761 7 5 4.573873445991964 7 6 4.86423640281668 7 7 4.37773024125552 7 8 4.651129167376858 7 9 4.676473052855691 7 10 5.0120510552275945 7 11 5.207140746859736 7 12 5.233208823662032 7 13 5.957232132002048 7 14 5.364614585816298 7 15 6.270497186925361 7 16 7.303200807333717 7 17 8.484745350661644 7 18 8.710249440173019 7 19 7.607963309503013 7 20 6.915518644138352 7 21 6.654549049685368 7 22 7.307719740987984 7 23 6.572541171164374 7 24 7.458619270132843 7 25 6.651225203064172 7 26 5.558161751979576 7 27 5.370902140521608 7 28 5.612327880372735 7 29 5.88430387622455 7 30 5.566247954866135 7 31 4.850824686930199 7 32 4.848185252587275 7 33 5.203441235997229 7 34 4.93674918434801 7 35 4.319246370532053 7 36 5.53298664932375 7 37 6.390560618234449 7 38 6.4945942363180755 7 39 7.611038402540888 7 40 6.543057382172702 7 41 10.085471477800866 7 42 10.047419207160495 7 43 10.056343958031604 7 44 10.74170855353291 7 45 10.200553047288473 7 46 11.922905154050245 7 47 11.557818531028403 7 48 11.106954261347845 7 49 11.243847590912996 7 50 11.257682787915751 7 51 10.301858013196945 7 52 11.106382954441756 7 53 10.570132599680559 7 54 10.689521200518914 7 55 10.4382360603676 7 56 9.891307433298415 8 1 5.0459013481237776 8 2 4.978697341645386 8 3 6.155060700473641 8 4 7.710477187669401 8 5 4.581708497538351 8 6 4.765164237868227 8 7 4.532060138162734 8 8 4.581335352943874 8 9 4.7828648054330145 8 10 4.83638095213481 8 11 5.051728375725716 8 12 5.632177895826985 8 13 5.770067183548434 8 14 5.748686750764747 8 15 6.448950795172777 8 16 7.040778126921346 8 17 8.342631948599784 8 18 8.607620574193636 8 19 7.4122416600184735 8 20 6.531807303932169 8 21 7.007229462056504 8 22 7.494730050266334 8 23 6.318056635081902 8 24 7.122124424772018 8 25 6.291225203064172 8 26 5.825429793216685 8 27 5.601572243614392 8 28 5.084750560785107 8 29 5.858376041173003 8 30 6.049134552804276 8 31 3.8223710786827816 8 32 4.338649170113047 8 33 4.579523710224034 8 34 4.306388359605748 8 35 4.1894525560990665 8 36 4.725151597777359 8 37 5.381849278028266 8 38 5.911759184771686 8 39 6.067275515942949 8 40 2.1493460419665134 8 41 10.408461168522514 8 42 9.190511990665648 8 43 8.90356045287696 8 44 10.445883811264864 8 45 9.016841707082285 8 46 11.074090721060553 8 47 11.876014407317065 8 48 11.15344910670867 8 49 11.89255893111918 8 50 10.502167323998226 8 51 9.47531162144437 8 52 8.983187078153094 8 53 9.739720228546538 8 54 9.608129447941597 8 55 9.032926782017086 8 56 8.830431144638624 9 1 5.035592069773259 9 2 5.035398372573226 9 3 6.0983596695458075 9 4 7.426972033030221 9 5 4.880677569703302 9 6 4.646607536837294 9 7 4.367111684554487 9 8 4.658654940572738 9 9 4.767400887907243 9 10 4.578648993371921 9 11 4.644511880880351 9 12 5.276507792734201 9 13 5.862850688703077 9 14 5.465181596125575 9 15 5.768538424038759 9 16 6.819128642385266 9 17 8.337477309424532 9 18 8.726177275224568 9 19 7.07719011362672 9 20 6.707065035890935 9 21 6.775270699169905 9 22 7.680297060575612 9 23 6.50362364539118 9 24 7.323155352607074 9 25 6.095348914404376 9 26 5.851202989092975 9 27 5.874768119903052 9 28 5.342482519547996 9 29 6.002705938080226 9 30 5.698619088886751 9 31 4.770824686930201 9 32 4.245865664958412 9 33 4.615606184450844 9 34 4.296079081255229 9 35 3.462648432387727 9 36 4.4364918039629195 9 37 4.9643235048323895 9 38 5.365367432194365 9 39 6.11882190769553 9 40 2.267902742997439 9 41 8.470316838625607 9 42 8.711130547366679 9 43 8.310776947722324 9 44 9.14691473909992 9 45 8.104470573061668 9 46 10.048317525184263 9 47 10.876014407317065 9 48 9.782315086090108 9 49 10.511115632150105 9 50 10.295981756987917 9 51 9.872218837939212 9 52 9.581125222482992 9 53 9.39435940380427 9 54 9.96895419020964 9 55 10.264885544903684 9 56 9.443833206494293 10 1 4.529041382488039 10 2 4.5823270667313025 10 3 5.681693002879143 10 4 7.065610349181423 10 5 4.127831779325298 10 6 4.364444736150013 10 7 4.168980241255518 10 8 4.209879167376862 10 9 4.007306386189029 10 10 4.65038438856093 10 11 4.45609908019307 10 12 4.848833823662034 10 13 4.779732132002046 10 14 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3.818482785682896 114 28 3.909908525534025 114 29 3.9538200052568087 114 30 4.31044150325323 114 31 3.6175988804785852 114 32 3.08931428484534 114 33 3.3740863972875523 114 34 3.925942732735109 114 35 3.889568951177214 114 36 4.435244713839875 114 37 4.908786424686067 114 38 5.162174881479363 114 39 6.493780338024759 114 40 7.99079931765657 114 41 7.983535993929898 114 42 6.990645013612106 114 43 6.076989119321926 114 44 -2.354581769047737 114 45 5.059907885998147 114 46 -0.3982238782078191 114 47 -3.2600846947780475 114 48 0.14663168070268284 114 49 -7.400507247796684 114 50 0.7559085943673622 114 51 2.7687934970679144 114 52 2.9579958576675622 114 53 3.016906793228948 114 54 2.686779265035046 114 55 3.793236060367601 114 56 4.937597755879061 115 1 3.988885530070732 115 2 3.82032006927583 115 3 4.903703180996189 115 4 5.781815819919341 115 5 3.4973848963736387 115 6 3.5548470898395763 115 7 3.4831500885837627 115 8 3.546472678827243 115 9 3.6639539688862257 115 10 3.970677009426069 115 11 1.1405376934246192 115 12 -2.510302626719646 115 13 -3.2760884786849758 115 14 -4.4951945744890445 115 15 -6.465151668036473 115 16 0.8645366851963132 115 17 2.079745350661643 115 18 3.3670051653638566 115 19 1.2899862102663704 115 20 1.7623888731459871 115 21 4.080732255792238 115 22 4.870467832591032 115 23 4.566777812385752 115 24 4.895871178529795 115 25 4.195805355735928 115 26 3.938123584040646 115 27 3.3947571023536653 115 28 3.5319843689223553 115 29 4.129761891491725 115 30 3.9618204739501053 115 31 3.0914353739531 115 32 3.0445592983888012 115 33 2.7151969611880737 115 34 3.167016359920531 115 35 3.2194753781656438 115 36 4.832375962300848 115 37 4.948308709837502 115 38 4.973182022577614 115 39 5.968786494143938 115 40 7.670003947058195 115 41 8.07089132512911 115 42 7.335510810213929 115 43 5.633748538184275 115 44 -1.1971082403602225 115 45 6.029866024387708 115 46 0.46714179527161903 115 47 -8.347563148360909 115 48 0.40641991020280344 115 49 -3.906724928170977 115 50 0.9595530169233832 115 51 1.8932702269374033 115 52 2.6647799010066393 115 53 2.6376898515889557 115 54 2.1239868493738783 115 55 2.443999419146227 115 56 6.350734914214446 116 1 4.399752166801761 116 2 4.366845040587506 116 3 5.225810649937962 116 4 6.686321133495152 116 5 4.369559720501769 116 6 4.332275618502955 116 7 4.325638737987546 116 8 4.528710866723266 116 9 4.327976320829549 116 10 4.832573931044585 116 11 3.970702838363003 116 12 3.4606598040541883 116 13 4.753996837884401 116 14 4.276346611960086 116 15 -3.0648622901988247 116 16 4.271860938052669 116 17 6.01438587353746 116 18 5.603746172199163 116 19 4.674858734339615 116 20 4.7012702781252855 116 21 5.551869311123273 116 22 6.159745884778829 116 23 5.493684962014051 116 24 6.299403583858336 116 25 5.843512784763519 116 26 5.3968872421756515 116 27 4.922372728756905 116 28 5.000726573183197 116 29 5.252048974263765 116 30 5.067293706500124 116 31 4.404811615034777 116 32 4.542139500953287 116 33 4.782003327500501 116 34 4.929526962125792 116 35 4.747939180989572 116 36 5.956908217951199 116 37 6.799678265293277 116 38 6.548744563115459 116 39 7.310352128031081 116 40 8.939724048839363 116 41 8.214033569304135 116 42 6.397876723500364 116 43 0.25451389267212576 116 44 2.496251037193037 116 45 5.653624942713307 116 46 0.47901626516135565 116 47 -5.001756632370288 116 48 1.201660143700785 116 49 -1.2344203829432168 116 50 1.1990880166739188 116 51 4.448426640647927 116 52 4.4753372028077685 116 53 4.175034560464873 116 54 4.291776102479702 116 55 4.327549785857798 116 56 6.715849916958547 117 1 3.8394580491547003 117 2 3.780243733397967 117 3 4.631693002879139 117 4 6.334360349181427 117 5 3.628873445991964 117 6 3.6342364028166756 117 7 3.204396907922188 117 8 3.541129167376859 117 9 3.503139719522359 117 10 3.8087177218942614 117 11 0.19880741352640285 117 12 -7.900124509671301 117 13 -6.7327678679979535 117 14 -1.670385414183702 117 15 -9.732836146407976 117 16 -8.753465859332952 117 17 -1.2369213160050236 117 18 -4.104750559826982 117 19 0.25296330950300927 117 20 -1.3694813558616445 117 21 5.259549049685365 117 22 5.402719740987983 117 23 4.484207837831043 117 24 5.44695260346618 117 25 4.916225203064172 117 26 4.966495085312907 117 27 4.662568807188272 117 28 4.578994547039404 117 29 4.655970542891218 117 30 5.24458128819947 117 31 3.6124913535968695 117 32 3.6815185859206103 117 33 4.510107902663901 117 34 4.335082517681343 117 35 3.957579703865388 117 36 5.494653315990416 117 37 4.78056061823445 117 38 5.272927569651404 117 39 5.8427050692075575 117 40 8.348057382172701 117 41 7.375471477800865 117 42 5.862419207160492 117 43 -4.170322708635066 117 44 -0.7932914464670944 117 45 5.298886380621806 117 46 -2.6154281792830893 117 47 -1.7805148023049284 117 48 -0.6213790719854906 117 49 -2.1728190757536723 117 50 0.6360161212490851 117 51 5.051858013196945 117 52 5.481382954441756 117 53 5.1451325996805615 117 54 5.7045212005189185 117 55 5.134902727034266 117 56 7.976307433298416 118 1 3.425719310415964 118 2 3.4761896793439107 118 3 4.652413723599864 118 4 5.982558547379622 118 5 3.234954527073043 118 6 3.2702724388527145 118 7 3.094937448462723 118 8 3.4393273655750605 118 9 3.1013379177205564 118 10 3.6785375417140784 118 11 -0.6417331270141418 118 12 -6.545169554716345 118 13 -7.2613714716015565 118 14 -9.724889918688206 118 15 -9.19770101127284 118 16 -8.175763156630246 118 17 -0.8031375322212391 118 18 -7.493939749016171 118 19 -0.9555952490555484 118 20 -1.659796671176963 118 21 3.5304949956313116 118 22 4.90519721846546 118 23 3.755829459452663 118 24 4.762042693556268 118 25 3.944378356217328 118 26 4.515594184412009 118 27 4.421577816197285 118 28 3.945435988480842 118 29 4.119033605954279 118 30 4.425887594505774 118 31 3.518572434677946 118 32 3.643005072407096 118 33 3.5209187134747104 118 34 3.088686121284951 118 35 3.205102226387911 118 36 4.65749115382825 118 37 4.586641699315532 118 38 5.096350993074832 118 39 6.823335699838186 118 40 8.318102427217742 118 41 5.970966973296363 118 42 4.641473261214546 118 43 -0.3144668527792085 118 44 0.4148166616410158 118 45 5.847760254495679 118 46 -0.4429056567605656 118 47 -0.39267696446709266 118 48 -0.8243069999134178 118 49 -0.316737994672593 118 50 1.5734035086364706 118 51 6.382713869052804 118 52 6.414040612099413 118 53 5.46968214923011 118 54 6.379746425744145 118 55 7.110803627935169 118 56 9.111893018884004 119 1 3.6148397285440197 119 2 3.3470376265277366 119 3 4.430420738248095 119 4 6.285632613812471 119 5 2.993568102480509 119 6 3.379274570755605 119 7 2.902997416828036 119 8 3.538839091040984 119 9 3.3967783963671465 119 10 4.016478536143623 119 11 0.05656822777576309 119 12 -5.914882779391402 119 13 -3.4058594710513894 119 14 -6.693667856931793 119 15 -7.640724187120444 119 16 -7.036226673582313 119 17 -0.38590350430018816 119 18 -6.7627658270025535 119 19 -0.9542885988939354 119 20 -0.7261607451746244 119 21 4.584549049685368 119 22 5.435353328774241 119 23 3.7118159803246797 119 24 3.2622833922702483 119 25 4.142370241232115 119 26 4.648047248162783 119 27 4.310787636704816 119 28 4.325877498693348 119 29 5.389303876224552 119 30 4.68701131364476 119 31 3.2135727785332513 119 32 3.0750936495338443 119 33 3.2113801672949442 119 34 3.197550711065567 119 35 2.9752005690053345 119 36 4.5804675653542795 119 37 4.910140770906207 119 38 4.92738049586006 119 39 6.243595654449283 119 40 7.028782573012396 119 41 5.162494378564226 119 42 4.007266535404767 119 43 -3.709762912197405 119 44 -1.0368028968487728 119 45 3.4573469404182404 119 46 -2.2504154566367767 119 47 -3.6834410109563294 119 48 -6.463809097430783 119 49 -4.395274546491585 119 50 -1.223653089946847 119 51 3.0917435093801515 119 52 3.3289020384112185 119 53 2.8819646607492615 119 54 2.360628070747925 119 55 3.436365831359968 119 56 5.358368502000708 120 1 3.6480646065317544 120 2 3.3417191432340303 120 3 4.0983596695458075 120 4 6.084906797268854 120 5 3.317807872221472 120 6 3.5211216487183137 120 7 3.4369105691243718 120 8 3.6263750690162055 120 9 3.3430304299048714 120 10 3.7658215470308747 120 11 -1.0301543351074791 120 12 -5.2815452746986224 120 13 -5.124980982752053 120 14 -6.359729676478786 120 15 -1.1388470753697248 120 16 -9.498684438567924 120 17 -1.4120579280268828 120 18 -8.60802924835157 120 19 -1.364249805251088 120 20 -1.3772682411075472 120 21 2.4124179021443872 120 22 4.903129577053555 120 23 3.910164121984053 120 24 3.5763241881656356 120 25 3.856798973555975 120 26 5.107751915914001 120 27 4.262295583144557 120 28 4.517655749225195 120 29 4.8811071549130745 120 30 4.810838118800561 120 31 4.483939441028564 120 32 3.355972137833181 120 33 4.543441235997232 120 34 4.397650823692274 120 35 3.7836726000402514 120 36 4.677576813258174 120 37 4.882609798562317 120 38 5.126479482219711 120 39 6.419481025491709 120 40 6.043549185451386 120 41 -5.368790817281102 120 42 -3.765039809232949 120 43 -8.741360960001185 120 44 -15.250668495647421 120 45 -4.515184657629561 120 46 -8.246848944310411 120 47 -7.159476550938809 120 48 -5.235176886193141 120 49 -14.895086835316514 120 50 -5.294858195690807 120 51 0.518661291885472 120 52 0.21539934788437876 120 53 0.3873457144346588 120 54 0.687308085764819 120 55 0.9366786833184193 120 56 5.370569728380385 >>END_MODULE >>Per sequence quality scores pass #Quality Count 4 3405.0 5 147.0 6 347.0 7 326.0 8 492.0 9 730.0 10 534.0 11 428.0 12 410.0 13 364.0 14 460.0 15 565.0 16 669.0 17 909.0 18 1032.0 19 1402.0 20 1771.0 21 2330.0 22 2799.0 23 3145.0 24 3672.0 25 3994.0 26 4185.0 27 4269.0 28 4506.0 29 4837.0 30 5497.0 31 6001.0 32 6725.0 33 7446.0 34 8422.0 35 9182.0 36 9821.0 37 10860.0 38 10882.0 39 9955.0 40 7.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 100.0 0.0 0.0 0.0 2 0.0 0.0 100.0 0.0 3 0.0 100.0 0.0 0.0 4 0.0 100.0 0.0 0.0 5 0.0 0.0 100.0 0.0 6 100.0 0.0 0.0 0.0 7 99.44539184763744 0.30937325505938457 0.06866577124488779 0.1765691260582829 8 99.09300816443566 0.5621538415103451 0.10035766566560524 0.2444803283883917 9 95.95394041833933 1.7498717213485857 1.5823548942078416 0.7138329661042528 10 50.60591883856753 23.562168932888643 18.735946153962242 7.095966074581591 11 31.93939302476495 32.69924392194739 16.221722529918658 19.139640523369 12 27.771908908440608 20.777055068439402 19.25131672275629 32.1997193003637 13 20.780073344098515 16.731811116309252 22.58047477476118 39.90764076483105 14 19.79460634139716 24.614792569005328 32.266121364864254 23.32447972473326 15 34.327645413403886 19.43267357505098 21.77353942251567 24.466141589029466 16 34.47335830363076 24.231257173926178 20.37923639219477 20.916148130248295 17 25.00679265789156 21.436873565994446 34.05612244897959 19.500211327134405 18 23.861732792055896 20.94079652294644 28.88716176448395 26.31030892051371 19 31.910010569228447 23.733957421108258 19.095576022950326 25.26045598671297 20 25.334783495343622 30.75627459421899 24.0243506370894 19.884591273347986 21 23.71761012933311 22.145843079848483 29.62362102530824 24.51292576551016 22 22.425033578316707 20.70386188370584 26.889063278149195 29.982041259828264 23 22.707242352444048 22.017566364336055 24.51292576551016 30.76226551770973 24 22.572538957853826 22.271440968946912 29.32800060370524 25.828019469494016 25 30.9700788589971 23.116628306229483 23.401124401011206 22.512168433762216 26 27.22887018232607 22.248310747046165 20.566230040391076 29.956589030236685 27 22.02135532297283 25.96242429734342 19.092172232039026 32.924048147644726 28 24.470469277031157 34.0386197112954 19.274390105868417 22.21652090580503 29 35.69042400423248 24.965611064923284 18.33950570629582 21.00445922454841 30 25.849076549026716 22.673250879656894 30.678506168924326 20.799166402392064 31 28.300932773870258 32.28333383644761 19.480333262897336 19.935400126784796 32 37.007380797850665 23.904577905906148 19.03913785036149 20.048903445881695 33 27.50774344640024 24.21167938354612 20.241746619324623 28.038830550729017 34 23.219495971094684 30.894069269959335 26.594554552738597 19.291880206207388 35 23.370567429486986 25.17231476163125 33.22727410139363 18.229843707488133 36 25.193438563912103 33.37938114757193 21.561700296668704 19.865479991847273 37 26.436251804051654 24.117998201615524 29.828246726260193 19.617503268072632 38 34.46163619038977 25.00945293948606 21.136772690835944 19.39213817928823 39 26.87581244898576 23.23528522627649 19.719459475800356 30.169442848937393 40 21.529833425916767 18.80559761971799 27.165501130025188 32.49906782434005 41 18.429844097995545 16.876163163193702 25.154834182506026 39.53915855630473 42 28.852483825810598 23.945367182475124 22.03321856910446 25.168930422609815 43 19.25220639950389 21.090683586807735 30.919087340901015 28.738022672787363 44 20.856976189213555 22.597898468927795 23.547905665952015 32.997219675906635 45 23.947581346460684 22.308175101895753 31.587305188176312 22.156938363467255 46 20.487133117939482 25.960759982142996 22.50890958754852 31.043197312369003 47 18.345640077024548 27.470319773171802 22.79805315906782 31.385986990735827 48 23.904574966027482 29.49192208968745 24.356032009663295 22.24747093462177 49 20.8101403678606 28.810200871248792 22.523142545982573 27.856516214908034 50 28.27043454594938 23.691372584595847 24.512383198766216 23.52580967068856 51 20.481736248268973 31.009406191589665 25.69184317464642 22.81701438549494 52 21.169223792608268 25.34577885269443 31.582646814299753 21.90235054039755 53 20.727333262708658 24.188446812052604 32.36224404860996 22.72197587662878 54 21.177911698210224 29.788810670618748 26.12305388995168 22.910223741219347 55 20.61785468384695 28.793874416457964 25.6478546081851 24.940416291509983 56 27.949408454113588 22.273745045235863 26.43801627885867 23.338830221791884 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 1.0 4 11.0 5 20.0 6 106.5 7 193.0 8 490.0 9 787.0 10 781.5 11 776.0 12 663.0 13 443.5 14 337.0 15 363.5 16 390.0 17 406.0 18 422.0 19 409.5 20 397.0 21 384.0 22 416.5 23 462.0 24 528.0 25 594.0 26 640.0 27 686.0 28 727.5 29 845.5 30 922.0 31 1050.0 32 1178.0 33 1393.0 34 1608.0 35 1771.5 36 1935.0 37 1984.0 38 2233.0 39 2433.0 40 2734.0 41 3035.0 42 4023.5 43 5012.0 44 8480.0 45 11948.0 46 10960.5 47 8489.0 48 7005.0 49 6743.0 50 6481.0 51 6872.0 52 7263.0 53 7480.5 54 7741.5 55 7785.0 56 7881.5 57 7978.0 58 8465.5 59 8953.0 60 8501.5 61 8050.0 62 7228.0 63 5911.0 64 5416.0 65 4615.0 66 3814.0 67 3439.5 68 3065.0 69 2619.5 70 2174.0 71 1904.5 72 1288.0 73 941.0 74 692.5 75 444.0 76 357.0 77 270.0 78 245.0 79 141.5 80 63.0 81 41.5 82 20.0 83 12.0 84 4.0 85 4.5 86 5.0 87 3.0 88 0.5 89 0.0 90 1.0 91 2.0 92 1.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0015091378295579736 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.45953246910040296 16 0.07696602930745665 17 0.022637067443369602 18 0.0 19 0.049801548375413125 20 0.09583025217693132 21 0.0 22 0.0 23 0.0 24 0.008300258062568854 25 0.008300258062568854 26 0.05357439294930806 27 0.07545689147789868 28 0.00226370674433696 29 0.16298688559226115 30 0.06640206450055083 31 0.013582240466021762 32 0.015091378295579737 33 0.11771275070552195 34 0.17505998822872493 35 0.1584594721035872 36 0.041501290312844274 37 0.13959524923411257 38 0.2203341231154641 39 0.1584594721035872 40 0.8398352021490123 41 1.0699787211566032 42 0.2799450673830041 43 0.22410696768935906 44 0.39765781808852607 45 0.21354300288245323 46 0.27541765389433015 47 0.4678327271629718 48 0.049801548375413125 49 0.227879812263254 50 0.1886422286947467 51 0.285981618701236 52 0.16298688559226115 53 0.2806996362977831 54 0.20750645156422134 55 0.27089024040565624 56 0.24900774187706565 >>END_MODULE >>Sequence Length Distribution pass #Length Count 56 132526.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 68.56390444139264 #Duplication Level Percentage of deduplicated Percentage of total 1 88.74264018048754 60.84541901211837 2 6.582292411819733 9.026153358586239 3 1.9039234028503822 3.916212667702941 4 0.9123424861057612 2.50215052140712 5 0.4611236449678094 1.5808218764619772 6 0.2608265008529137 1.0729969968157191 7 0.20139767787376878 0.9666027798318819 8 0.15517526000110052 0.851153735870697 9 0.1111539096461784 0.685903143534099 >10 0.5931876960325758 7.232543048156588 >50 0.046222417872668245 2.164858216500913 >100 0.023111208936334122 3.0054479875647044 >500 0.004402135035492213 1.820020222446916 >1k 0.0022010675177461065 4.329716433001826 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTAATGGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAG 4617 3.483844679534582 No Hit GTAATGGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTCGTCTCAAAGATTAAG 1121 0.8458717534672441 No Hit GTAATGGGGTCACAGATCCTCTTCTGAGATGAGTTTTTGTTCAATCCGCCCAATCT 712 0.5372530673226386 No Hit GTAATGGGGTACCTGGTTGATCCTGCCAGTAGCATATGCCTGTCTCAAAGATTAAG 579 0.43689540165703333 No Hit GTAATGGGGTCACAGATCCTCTTCTGAAGGAGATTGGGCGGATTGAACAAAAACTC 568 0.4285951435944645 No Hit GTAATGGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCCAAGATTAAG 553 0.41727660987277965 No Hit GTAATGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 436 0.3289920468436382 No Hit GTAATGGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCCACGATTAAG 369 0.2784359295534461 No Hit GTAATGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC 325 0.2452348973031707 No Hit GTAATGGGGGGGCGCTGACCCCCCTTCCCGGGGGGGGATGCGTGCATTTATCAGAT 253 0.19090593543908366 No Hit GTAATGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACAAAAAAAAAAAAAAA 231 0.17430541931394594 No Hit GTAATGGGGTACCTGGTTGATCCTGCCAGTAGCATATGCCGTCTTCTGCTTGGATC 227 0.17128714365483 No Hit GTAATGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTC 222 0.16751429908093507 No Hit GTAATGGGGTCACAGATCCTCTTCTGAAGGAGATTGGGCGCATTGAACAAAAACTC 218 0.1644960234218191 No Hit GTAATGGGGCTCCTACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCGAC 188 0.1418589559784495 No Hit GTAATGGGGTCACAGATCCTCTTCTGAGATGAGTTTTTGTCCAATCCGCCCAATCT 170 0.12827671551242775 No Hit GTAATGGGGGGTTCCTTTGGTCGCTCGCTCCTCTCCTACTTGGATAACTGTGGTAA 147 0.11092163047251105 No Hit GTAATGGGGATGCCACAACTAGATACATCAACATGATTTATCACAATTATCTCATC 147 0.11092163047251105 No Hit GTAATGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTTCTTCC 146 0.11016706155773205 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 7.545689147789868E-4 0.0 0.0 0.0 0.0 10 0.003018275659115947 0.0 0.0 0.0 0.0 11 0.0052819824034529075 0.0 0.0 0.0 0.0 12 0.0052819824034529075 0.0 0.0 0.0 0.0 13 0.0052819824034529075 0.0 0.0 0.0 0.0 14 0.012073102636463788 0.0 0.0 0.0 0.0 15 0.012073102636463788 0.0 0.0 0.0 0.0 16 0.012827671551242774 0.0 0.0 0.0 0.0 17 0.013582240466021762 0.0 0.0 0.0 0.0 18 0.015091378295579735 0.0 0.0 0.0 0.0 19 0.019618791784253655 0.0 0.0 0.0 0.0 20 0.021882498528590616 0.0 0.0 0.0 0.0 21 0.02565534310248555 0.0 0.0 0.0 0.0 22 0.02791904984682251 0.0 0.0 0.0 0.0 23 0.0339556011650544 0.0 0.0 0.0 0.0 24 0.03923758356850731 0.0 0.0 0.0 0.0 25 0.04074672139806529 0.0 0.0 0.0 0.0 26 0.05055611729019211 0.0 0.0 0.0 0.0 27 0.056592668608424004 0.0 0.0 0.0 0.0 28 0.06564749558577185 0.0 0.0 0.0 0.0 29 0.07017490907444576 0.0 0.0 0.0 0.0 30 0.07243861581878273 0.0 0.0 0.0 0.0 31 0.07772059822223563 0.0 0.0 0.0 0.0 32 0.07998430496657259 0.0 0.0 0.0 0.0 33 0.08149344279613058 0.0 0.0 0.0 0.0 34 0.08300258062568855 0.0 0.0 0.0 0.0 35 0.08375714954046753 0.0 0.0 0.0 0.0 36 0.08602085628480449 0.0 0.0 0.0 0.0 37 0.08979370085869942 0.0 0.0 0.0 0.0 38 0.09054826977347841 0.0 0.0 0.0 0.0 39 0.09281197651781538 0.0 0.0 0.0 0.0 40 0.09356654543259436 0.0 0.0 0.0 0.0 41 0.09507568326215234 0.0 0.0 0.0 0.0 42 0.09884852783604726 0.0 0.0 0.0 0.0 43 0.1033759413247212 0.0 0.0 0.0 0.0 44 0.11318533721684801 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGATTGA 15 0.008139209 50.422462 40 ACCTATG 15 0.008139209 50.422462 40 CTCGGAT 15 0.008139209 50.422462 39 CACGATT 35 5.1775714E-8 50.07815 47 CTTAGGT 15 0.008362771 50.078148 14 TAGATCC 15 0.008375323 50.059162 13 ACGCTTA 15 0.008400471 50.02122 11 GATTACG 15 0.008400471 50.02122 50 TGATGTC 15 0.008400471 50.02122 12 GTCATAG 15 0.008438297 49.96442 9 GCAAAAC 20 4.1850258E-4 49.96442 9 GTACGCT 15 0.008438297 49.96442 9 GACGCAC 25 2.0861258E-5 49.964417 9 GTCATTA 15 0.008488929 49.88889 29 CTACTGA 15 0.008488929 49.88889 31 GATAATG 15 0.008514329 49.85121 28 TTGCGGT 15 0.008514329 49.85121 27 ATGGGGG 6470 0.0 49.467125 4 TGGGGTA 1165 0.0 49.386 5 AATGGGG 12665 0.0 49.12528 3 >>END_MODULE