FastQCFastQC Report
Fri 17 Jun 2016
SRR1526157_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526157_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5071
Sequences flagged as poor quality0
Sequence length40
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGC110.21691973969631237No Hit
GTAATGGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTT100.19719976336028394No Hit
GTAATGGGGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTT100.19719976336028394No Hit
GTAATGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA90.17747978702425557No Hit
GTAATGGGGGGGCTGGTGCGATGGCTCAGTGGGTAAGAGC90.17747978702425557No Hit
GTAATGGGGGTGCAGTGCTGTAATGGGGAAAAAAAAAAAA90.17747978702425557No Hit
GTAATGGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCG90.17747978702425557No Hit
GTAATGGGGGGGGTTGGGGATTTAGCTCCGTGGTAGAGCG80.1577598106882272No Hit
GTAATGGGGGTCGACTTTTTTTTTTTTTTTTTTTTTTTTT80.1577598106882272No Hit
GTAATGGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCC80.1577598106882272No Hit
GTAATGGGGGGGGTTGGGGATTTAGCTCCGTGGTAGAGCC60.11831985801617037No Hit
GTAATGGGGAAAAAAAAACAAAAAAAAAAAAAAAAAAAAA60.11831985801617037No Hit
GTAATGGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTT60.11831985801617037No Hit
GTAATGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTT60.11831985801617037No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATGAGAT200.00244704534.2420
ATGGGGC800.033.900994
GGATGAG200.002571995433.9009918
GCAGGGA251.7878077E-433.9009914
AGGGATG200.002571995433.9009916
GATGAGA200.002571995433.9009919
TGGGGTG301.2379123E-533.900995
TGGGGGT301.2379123E-533.900995
TGGGGGG700.033.900995
TGGGGGC358.533061E-733.900995
TGGGGCG251.7878077E-433.900995
TGGGGCC200.002571995433.900995
TGGGGCA200.002571995433.900995
TGGGGAG502.6921043E-1033.900995
CAGGGAT200.002571995433.9009915
GGGAAGC200.002571995433.900997
GGGGATG251.7878077E-433.900996
GGGGAGA251.7878077E-433.900996
GGGGAAG301.2379123E-533.900996
ATGGGGG1700.032.9039044
Produced by FastQC (version 0.11.5)