FastQCFastQC Report
Fri 17 Jun 2016
SRR1526156_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526156_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1715
Sequences flagged as poor quality0
Sequence length40
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATGGGGGCCTTTTTTTTTTTTTTTTTTTTTTTTTTTT40.23323615160349853No Hit
GTAATGGGGCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTT40.23323615160349853No Hit
GTAATGGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTT40.23323615160349853No Hit
GTAATGGGGGAGTCGCCTTTTTTTTTTTTTTTTTTTTTTT40.23323615160349853No Hit
GTAATGGGGGTCGCCTTTTTTTTTTTTTTTTTTTTTTTTT40.23323615160349853No Hit
GTAATGGGGGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT30.1749271137026239No Hit
GTAATGGGGGTCGACTTTTTTTTTTTTTTTTTTTTTTTTT30.1749271137026239No Hit
GTAATGGGGGGGCTGGTGAGATGGCCCAGTGGGTAAGAGC30.1749271137026239No Hit
GTAATGGGGGGGCTGGTGAGATGGCCCCGTGGGTACGAGC30.1749271137026239No Hit
GTAATGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA20.11661807580174927No Hit
GTAATGGGGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTT20.11661807580174927No Hit
GTAATGGGGGACTTTTTTTTTTTTTTTTTTTTTTTTTTCC20.11661807580174927No Hit
GTAATGGGGAGAGTCGACTTTTTTTTTTTTTTTTTTTTTT20.11661807580174927No Hit
GTAATGGGGTGTCGACTTTTTTTTTTTTTTTTTTTTTTTT20.11661807580174927No Hit
GTAATGGGGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20.11661807580174927No Hit
GTAATGGGGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTT20.11661807580174927No Hit
GTAATGGGGGGGGCTGGTGAGATGGCCCAGTGGGTAAGAG20.11661807580174927No Hit
GTAATGGGTTCAGTCACCTTTTTTTTTTTTTTTTTTTTTT20.11661807580174927No Hit
GTAATGGGGGGGCTGGAGAGATGGCCCAGCGGTTAAGAGC20.11661807580174927No Hit
GTAATGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAG20.11661807580174927No Hit
GTAATGGGGGGGGCTGGTGAGATGGCTCCGTGGGTAAGAG20.11661807580174927No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATGGGGA451.5334081E-933.8823554
TAATGGG1500.033.8823552
ATGGGGG508.185452E-1133.882354
ATGGGGC354.8029506E-733.882354
TGGGGGG251.3272144E-433.882355
TGGGGGA251.3272144E-433.882355
AATGGGG1400.033.882353
GTAATGG1650.033.882351
TGGGGAG303.8811896E-428.2352925