FastQCFastQC Report
Fri 17 Jun 2016
SRR1526155_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526155_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7737
Sequences flagged as poor quality0
Sequence length40
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATGGGGTACCTGGTTGATCCTGCCAGTAGCATATGCT3644.704665891172289No Hit
GTAATGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1351.7448623497479645No Hit
GTAATGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGC911.1761664727930723No Hit
GTAATGGGGTCACAGATCCTCTTCTGAAGGAGATTGGGCG470.6074705958381802No Hit
GTAATGGGGTCACAGATCCTCTTCTGAGATGAGTTTTTGT450.5816207832493214No Hit
GTAATGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA370.4782215328938865No Hit
GTAATGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAG280.36189737624402224No Hit
GTAATGGGGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA210.27142303218301667No Hit
GTAATGGGGTACCTGGTTGATCCTGCCAGTAGCATATGCC200.2584981258885873No Hit
GTAATGGGGTAACTGGTTGATCCTGCCAGTAGCATATGCT170.21972340700529921No Hit
GTAATGGGGATGCCACAACTAGATACATCAACATGATTTA150.19387359441644048No Hit
GTAATGGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAACG140.18094868812201112No Hit
GTAATGGGGACTAATCCTAGCCCTAGCCCTACACAAATAT140.18094868812201112No Hit
GTAATGGGGTCACAGATCCTCTGAAGGAGATTGGGCGGAT120.15509887553315238No Hit
GTAATGGGGGTGTTCTTTATTAATATCCTAACACTCCTCG120.15509887553315238No Hit
GTAATGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCA120.15509887553315238No Hit
GTAATGGGGTCAAAGATCCTCTTCTGAGATGAGTTTTTGT120.15509887553315238No Hit
GTAATGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA120.15509887553315238No Hit
GTAATGGGGAAAAAAAAAAAACAAAAAAAAAAAAAAAAAA110.14217396923872302No Hit
GTAATGGGGTCAAAGATCCTCTTCTGAAGGAGATTGGGCG100.12924906294429364No Hit
GTAATGGGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA100.12924906294429364No Hit
GTAATGGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTT100.12924906294429364No Hit
GTAATGGGGATGCCATCTACCTTCTTCAACCTCACCATAG90.11632415664986429No Hit
GTAATGGGGTACATGGTTGATCCTGCCAGTAGCATATGCT90.11632415664986429No Hit
GTAATGGGGGGGCTAGAGAGATGGCTCAGAGGTTAAGAGC90.11632415664986429No Hit
GTAATGGGGCCTATGTTCATTAATCGTTGATTATTCTCAA80.10339925035543493No Hit
GTAATGGGGCTCCTACTTGGATAACTGTGGTAATTCTAGA80.10339925035543493No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGAGAT251.7912926E-434.1764724
CCAGTAG700.034.1764726
CCTGCCA601.8189894E-1234.1764722
CAGTAGC700.034.1764727
GCCAGTA700.034.1764725
TGCCAGT650.034.1764724
TCTGAGA251.7912926E-434.1764723
AGATGAG251.7912926E-434.1764727
CTGCCAG650.034.1764723
TCCTGCC601.8189894E-1234.1764721
ATCCTGC601.8189894E-1233.95454820
TGGTTGA800.033.95454814
ACAGATC301.31412E-533.95454812
TGATCCT601.8189894E-1233.95454818
TAAGAGC200.002638527433.95454834
TGGGGGT800.033.9545485
TGGGGGG1200.033.9545485
TGGGGGC200.002638527433.9545485
TGGGGGA601.8189894E-1233.9545485
TGGGGCA301.31412E-533.9545485
Produced by FastQC (version 0.11.5)