FastQCFastQC Report
Fri 17 Jun 2016
SRR1526154_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526154_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26382
Sequences flagged as poor quality0
Sequence length56
%GC55

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTAGAGGGTCACAGATCCTCTTCTGAGATGAGTTTTTGTTCAATCCGCCCAATCT3621.3721476764460618No Hit
CCTAGAGGGTCACAGATCCTCTTCTGAAGGAGATTGGGCGGATTGAACAAAAACTC1840.6974452278068379No Hit
CCTAGAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC1350.5117125312713214No Hit
CCTAGAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTC800.30323705556819047No Hit
CCTAGAGGGTCACAGATCCTCTTCTGAGATGAGTTTTTGTCCAATCCGCCCAATCT740.2804942764005761No Hit
CCTAGAGGGTCACAGATCCTCTTCTGAAGGAGATTGGGCGCATTGAACAAAAACTC690.2615419604275642No Hit
CCTAGAGGGGAAACCAGCAGCCCCAAATCCTGCAGCAGAGCCCCAAAACTGGCCTG630.23879918125994998No Hit
CCTAGAGGGACTAATCCTAGCCCTAGCCCTACACAAATATAATTATACTATTATAT560.21226593889773332No Hit
CCTAGAGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGC500.18952315973011902No Hit
CCTAGAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCCCCCGACTGCTCTTCC480.18194223334091425No Hit
CCTAGAGGGTCACAGATCCTCTTCTGAGATGAGTTTTTGTTCAATCCGCCCAAAAA460.17436130695170948No Hit
CCTAGAGGGGGGGGCTGGTTGGCTCAGTGGTTAAGAGCACTGACTGCTCCTCCAAA440.16678038056250474No Hit
CCTAGAGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAG430.16298991736790236No Hit
CCTAGAGGGGGGCAGGGATTGGGACTGTTTTTGGGAGTCTCATCATTGGCTATGCC380.14403760139489044No Hit
CCTAGAGGGACTAATCCTAGCCCTAGCCCTACACAAATATCATTATACTATTATAT370.14024713820028806No Hit
CCTAGAGGGGGGGCTGGAGAGATGGCTCAGTGGTTAAGAGCACTGACTGCTATTCC290.10992343264346904No Hit
CCTAGAGGGGCTTCTTTCCGGTTTCCGCGGCAGCCGCAGCCATGAGCAGCAAAGTC280.10613296944886666No Hit
CCTAGAGGGGGCAAGCTCCGTTGGGCGCCTTGGATCCATTTCCATCTGGTTCTGCT270.10234250625426426No Hit
CCTAGAGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAGGGTG270.10234250625426426No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCAATCC309.081414E-750.76310741
CATTGAA203.8117444E-450.76310741
CATCATT150.0078537250.76310741
CTAGCAA251.8998571E-550.56672746
CCTAGCA251.8998571E-550.56672745
TGTCCAA309.454816E-750.46911238
GTTCCCT203.9230127E-450.4691139
TAGCAAG251.9218067E-550.4691147
GCGCATT251.9218067E-550.4691138
CGCATTG251.9218067E-550.4691139
TTGTCCA309.582163E-750.3718737
TTGTTCC203.9606701E-450.3718737
GGCGCAT251.9439658E-550.3718737
GCCTAGC251.9439658E-550.3718744
ATCATTG150.00809833950.3718742
TGGGCGC251.9439658E-550.3718735
TGGGCCG203.9606701E-450.3718735
AGTCTCA150.00809833950.3718736
TCATTGG150.00809833950.3718743
TTGCCTA251.9439658E-550.3718742
Produced by FastQC (version 0.11.5)