FastQCFastQC Report
Fri 17 Jun 2016
SRR1526153_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526153_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2047
Sequences flagged as poor quality0
Sequence length40
%GC54

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTAGAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGC90.4396678065461651No Hit
CCTAGAGGGGAGCCTTGTTTGCTAATTACTTTGTACTCTC50.24425989252564728No Hit
CCTAGAGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCG50.24425989252564728No Hit
CCTAGAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAG40.19540791402051783No Hit
CCTAGAGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTT40.19540791402051783No Hit
CCTAGAGGGATCGCAGAGCCGACTCTTTTTTTTTTTTTTT30.14655593551538837No Hit
CCTAGAGGGGGGGCTGGACAGATGGCTCAGTGGTTAAGAG30.14655593551538837No Hit
CCTAGAGGGGGGAATATTCCTCCTGATCTATTTGCTAGAA30.14655593551538837No Hit
CCTAGAGGGAAGATGTATCACGCAGATCGACTTTTTTTTT30.14655593551538837No Hit
CCTAGAGGGGGTTTAGTGAAATGCCTGCTTTGTTGTGTAG30.14655593551538837No Hit
CCTAGAGGGGGGGCTGGAGAGATGGCTCAGTGGTTAAGAG30.14655593551538837No Hit
CCTAGAGGGGGGGCTGGTGAGATGGCTCCGTGGGTAAGAG30.14655593551538837No Hit
CCTAGAGGGGTGAATGTTGCCGGCGTCTCTCTGAAGTCTC30.14655593551538837No Hit
CCTAGAGGGGGGCTGGTGAGATGGCTCCGTGGGTAAGAGC30.14655593551538837No Hit
CCTAGAGGGGGGCTGGTGCGATGGCTCAGTGGGTAAGAGC30.14655593551538837No Hit
CCTAGAGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGC30.14655593551538837No Hit
CCTAGAGGGGATCCCCTCGACCACTCCTTTGGCGCTTCGC30.14655593551538837No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGGGGG251.4381566E-433.8256
AGGGGAG200.002233637733.8256
AGGGGAC200.002233637733.8256
AGAGGGG1050.033.8254
AGGGAAG200.002233637733.8256
TAGAGGG1850.033.8253
CTAGAGG1900.033.8252
GAGGGGA555.456968E-1233.8255
GAGGGAA200.002233637733.8255
CCTAGAG1950.033.8251
GAGGGGG355.581787E-733.8249975
AGAGGGA601.6370905E-1131.006254
Produced by FastQC (version 0.11.5)