FastQCFastQC Report
Fri 17 Jun 2016
SRR1526151_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526151_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1223
Sequences flagged as poor quality0
Sequence length40
%GC51

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTAGAGGGTCACAGATCCTCTTCTGAGATGAGTTTTTGT211.7170891251022076No Hit
CCTAGAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGC141.1447260834014716No Hit
CCTAGAGGGTCACAGATCCTCTTCTGAAGGAGATTGGGCG120.9811937857726901No Hit
CCTAGAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAG110.8994276369582993No Hit
CCTAGAGGGTCAAAGATCCTCTTCTGAGATGAGTTTTTGT60.49059689288634506No Hit
CCTAGAGGGGTGGGTTGGTGAGATGGCTCAGTGGGTAAGA60.49059689288634506No Hit
CCTAGAGGGACTAATCCTAGCCCTAGCCCTACACAAATAT50.4088307440719542No Hit
CCTAGAGGGGGGCAGGGATTGGGACTGTTTTTGGGAGTCT50.4088307440719542No Hit
CCTAGAGGGGGGGCTGGAGAGATGGCTCAGTGGTTAAGAG40.3270645952575634No Hit
CCTAGAGGGGGGAAGGGATTGGGACTGTTTTTGGGAGTCT40.3270645952575634No Hit
CCTAGAGGGAAGAATGTTATGTTTACTCCCACTTCGCCAT30.24529844644317253No Hit
CCTAGAGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCA30.24529844644317253No Hit
CCTAGAGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGC30.24529844644317253No Hit
CCTAGAGGGTCAAAGATCCTCTTCTGAAGGAGATTGGGCG30.24529844644317253No Hit
CCTAGAGGGGGGGCTGAAGAGATGGCTCAGCGGTTAAGAG20.1635322976287817No Hit
CCTAGAGGGGTTTGCCAAACTTCTTGATCTTGGGAGCCAG20.1635322976287817No Hit
CCTAGAGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGA20.1635322976287817No Hit
CCTAGAGGGCGTCTCATGGCCGCTAGTAGATCCCTGGTGC20.1635322976287817No Hit
CCTAGAGGGGCCCTTTCTTGGTGTGTGGGGGATCTATGGC20.1635322976287817No Hit
CCTAGAGGGGGGTTAGAGAGATGGCTTAGTGGTTAAGAGC20.1635322976287817No Hit
CCTAGAGGGATGCCACAACTAGATACATCAACATGATTTA20.1635322976287817No Hit
CCTAGAGGGGGGGCTGGCGACACGGCTCAGTGGTTAAGAG20.1635322976287817No Hit
CCTAGAGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCG20.1635322976287817No Hit
CCTAGAGGGTCACAGATCCTCTTATGAGATGAGTTTTTGT20.1635322976287817No Hit
CCTAGAGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAACG20.1635322976287817No Hit
CCTAGAGGGCTCTATGAAGACCATGGAGACACCCTTTCCC20.1635322976287817No Hit
CCTAGAGGGTACGAATTCTAAAGCAATCATTTTTGTGTGG20.1635322976287817No Hit
CCTAGAGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTT20.1635322976287817No Hit
CCTAGAGGGGCCTCTTCTGAGATGAGTTTTTGTTCAATCT20.1635322976287817No Hit
CCTAGAGGGGTGTAGTAACTCTCTAAAATGTCTGGAGATG20.1635322976287817No Hit
CCTAGAGGGAATGAGTTGGGGGGTAACCCCTAATCTGTAT20.1635322976287817No Hit
CCTAGAGGGGACTTGCTGTTGTCCAGAATTCACTTACCCC20.1635322976287817No Hit
CCTAGAGGGTCACGGATCCTCTTCTGAGATGAGTTTTTGT20.1635322976287817No Hit
CCTAGAGGGAGGGCTGGTGAGATGGCTCAGCGGTTAAGAT20.1635322976287817No Hit
CCTAGAGGGGAGCCTTAGGTTTTATTTTCTTATTTACAGT20.1635322976287817No Hit
CCTAGAGGGGGCCTTTCAGGAACACCACGACGCTACTCAG20.1635322976287817No Hit
CCTAGAGGGTCCCAGATCCTCTTCTGAAGGAGATTGGGCG20.1635322976287817No Hit
CCTAGAGGGGTGTCATTTTATATATGTTCCTAATGCTTAC20.1635322976287817No Hit
CCTAGAGGGGTCACAGATCCTCTTCTGAAGGAGATTGGGC20.1635322976287817No Hit
CCTAGAGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAA20.1635322976287817No Hit
CCTAGAGGGATGATTGAAAGCATAGAAGCCAATGTAGAAA20.1635322976287817No Hit
CCTAGAGGGGGGAATTAAATGTGCAATTAAATGCTCTGTG20.1635322976287817No Hit
CCTAGAGGGATTTATGGGATTTCTTCCCAGTATGGATCTT20.1635322976287817No Hit
CCTAGAGGGTACCTGGTTGATCCTGCCAGTAGCATATGCT20.1635322976287817No Hit
CCTAGAGGGGGTCTAACCGGAATTGTTTTATCCAACTCAT20.1635322976287817No Hit
CCTAGAGGGGCCGGTAGTGGTGGCGCACGCCGGTAGGATT20.1635322976287817No Hit
CCTAGAGGGGGGGCTGGTGAGATGGCTCAGTGAGTAAGAG20.1635322976287817No Hit
CCTAGAGGGTCACAGATCCTCTTATGAAGGAGATTGGGCG20.1635322976287817No Hit
CCTAGAGGGCGTCTGTGGAGCAGCTCCTGTCTCCCAACTA20.1635322976287817No Hit
CCTAGAGGGGGGATGGTGAGATGGCTCAGTGGGTAAGAGC20.1635322976287817No Hit
CCTAGAGGGGGTCATGGTGGGCCTCTACTTGCTCATCTAC20.1635322976287817No Hit
CCTAGAGGGGTGTTCTTGTTCCTGTCAGCAGCACATTGCG20.1635322976287817No Hit
CCTAGAGGGGGTGGTCCCTGTGTTAATCGGGGCTGAGGGT20.1635322976287817No Hit
CCTAGAGGGGTCAGTGACGAGAGGCTTTGTCCGGTTCGGC20.1635322976287817No Hit
CCTAGAGGGGAAGACCCGCCTTAATTGTGGGCGGAGCCGT20.1635322976287817No Hit
CCTAGAGGGGCGCTCTGAGCTGTTTTGTTGAAGTAGAAAA20.1635322976287817No Hit
CCTAGAGGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGC20.1635322976287817No Hit
CCTAGAGGGGGGCTGCCACAGTGACCTTGACCTAGCGAGG20.1635322976287817No Hit
CCTAGAGGGTCTGCTTCTTATATATTATTACAGTTTGCAC20.1635322976287817No Hit
CCTAGAGGGGGCTGGTGAAATGGCTCAGTGGGTAAGAGCA20.1635322976287817No Hit
CCTAGAGGGGCAATAATGTCTATTGGCTTTCTAGGCTTTA20.1635322976287817No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGAGGG1200.034.03
CTAGAGG1200.034.02
CCTAGAG1200.034.01
AGAGGGG700.033.9999964
AGAGGGC200.001823720633.9999964
GAGGGGG200.001823720633.9999965
GAGGGGA200.001823720633.9999965
Produced by FastQC (version 0.11.5)