##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1526151_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1223 Sequences flagged as poor quality 0 Sequence length 40 %GC 51 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 15.578086672117744 4.0 4.0 30.0 4.0 33.0 2 12.955846279640229 4.0 4.0 26.0 4.0 30.0 3 10.400654129190515 4.0 4.0 15.0 4.0 28.0 4 9.159443990188063 4.0 4.0 11.0 4.0 26.0 5 10.567457072771873 4.0 4.0 18.0 4.0 29.0 6 8.714636140637776 4.0 4.0 9.0 4.0 27.0 7 9.8454619787408 4.0 4.0 15.0 4.0 27.0 8 9.906786590351594 4.0 4.0 14.0 4.0 28.0 9 9.878986099754702 4.0 4.0 13.0 4.0 29.0 10 9.144726083401471 4.0 4.0 13.0 4.0 25.0 11 9.723630417007358 4.0 4.0 13.0 4.0 29.0 12 9.819296811120196 4.0 4.0 13.0 4.0 29.0 13 9.699100572363042 4.0 4.0 11.0 4.0 29.0 14 9.653311529026983 4.0 4.0 11.0 4.0 30.0 15 9.622240392477515 4.0 4.0 6.0 4.0 31.0 16 8.209321340964841 4.0 4.0 4.0 4.0 25.0 17 7.917416189697465 4.0 4.0 4.0 4.0 23.0 18 7.645952575633688 4.0 4.0 4.0 4.0 22.0 19 7.630417007358953 4.0 4.0 4.0 4.0 22.0 20 7.53556827473426 4.0 4.0 4.0 4.0 22.0 21 7.29926410466067 4.0 4.0 4.0 4.0 20.0 22 7.212591986917416 4.0 4.0 4.0 4.0 20.0 23 7.459525756336877 4.0 4.0 4.0 4.0 23.0 24 7.225674570727719 4.0 4.0 4.0 4.0 20.0 25 7.617334423548651 4.0 4.0 4.0 4.0 24.0 26 7.478331970564186 4.0 4.0 4.0 4.0 23.0 27 7.546197874080131 4.0 4.0 4.0 4.0 23.0 28 7.7219950940310715 4.0 4.0 4.0 4.0 25.0 29 7.116107931316435 4.0 4.0 4.0 4.0 20.0 30 7.418642681929681 4.0 4.0 4.0 4.0 23.0 31 7.632052330335241 4.0 4.0 4.0 4.0 26.0 32 7.560098119378577 4.0 4.0 4.0 4.0 26.0 33 7.436631234668847 4.0 4.0 4.0 4.0 24.0 34 7.508585445625511 4.0 4.0 4.0 4.0 25.0 35 7.458708094848733 4.0 4.0 4.0 4.0 25.0 36 7.152085036794767 4.0 4.0 4.0 4.0 22.0 37 6.982011447260834 4.0 4.0 4.0 4.0 20.0 38 6.793949304987735 4.0 4.0 4.0 4.0 18.0 39 6.808667211774325 4.0 4.0 4.0 4.0 17.0 40 6.814390842191333 4.0 4.0 4.0 4.0 18.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1 1 -10.267676767676766 1 2 -7.154040404040403 1 3 -4.255050505050505 1 4 -3.8661616161616177 1 5 -4.965909090909092 1 6 -4.3345959595959584 1 7 -4.521464646464647 1 8 -4.88510101010101 1 9 -4.67550505050505 1 10 -4.371212121212121 1 11 -4.479797979797979 1 12 -4.159090909090908 1 13 -4.296717171717171 1 14 -4.380050505050505 1 15 -4.44949494949495 1 16 -3.342171717171718 1 17 -3.05429292929293 1 18 -2.6704545454545467 1 19 -3.0189393939393945 1 20 -3.037878787878788 1 21 -3.188131313131314 1 22 -3.0277777777777777 1 23 -3.128787878787879 1 24 -2.4974747474747474 1 25 -2.8611111111111107 1 26 -2.896464646464646 1 27 -3.2361111111111116 1 28 -3.258838383838384 1 29 -2.691919191919192 1 30 -3.041666666666667 1 31 -2.976010101010101 1 32 -3.1401515151515147 1 33 -2.5315656565656557 1 34 -3.066919191919192 1 35 -2.7348484848484844 1 36 -2.621212121212121 1 37 -2.5 1 38 -2.3219696969696972 1 39 -2.4116161616161618 1 40 -2.3686868686868685 2 1 14.732323232323234 2 2 13.845959595959597 2 3 4.744949494949495 2 4 9.133838383838382 2 5 12.034090909090908 2 6 1.6654040404040416 2 7 9.478535353535353 2 8 6.11489898989899 2 9 -0.6755050505050502 2 10 16.62878787878788 2 11 7.520202020202021 2 12 2.8409090909090917 2 13 3.7032828282828287 2 14 17.619949494949495 2 15 13.55050505050505 2 16 20.657828282828284 2 17 1.94570707070707 2 18 23.329545454545453 2 19 20.981060606060606 2 20 9.962121212121211 2 21 9.811868686868685 2 22 7.972222222222222 2 23 2.871212121212121 2 24 1.5025252525252526 2 25 2.1388888888888893 2 26 20.103535353535353 2 27 21.76388888888889 2 28 11.741161616161616 2 29 19.30808080808081 2 30 19.958333333333332 2 31 27.0239898989899 2 32 25.859848484848484 2 33 10.468434343434344 2 34 19.93308080808081 2 35 23.265151515151516 2 36 13.378787878787879 2 37 11.5 2 38 6.678030303030303 2 39 23.588383838383837 2 40 13.631313131313131 3 1 -10.267676767676766 3 2 -7.154040404040403 3 3 -4.255050505050505 3 4 -3.8661616161616177 3 5 -4.965909090909092 3 6 -4.3345959595959584 3 7 -4.521464646464647 3 8 -4.88510101010101 3 9 -4.67550505050505 3 10 -4.371212121212121 3 11 -4.479797979797979 3 12 -4.159090909090908 3 13 -4.296717171717171 3 14 -4.380050505050505 3 15 -4.44949494949495 3 16 -3.342171717171718 3 17 -3.05429292929293 3 18 -2.6704545454545467 3 19 -3.0189393939393945 3 20 -3.037878787878788 3 21 -3.188131313131314 3 22 -3.0277777777777777 3 23 -3.128787878787879 3 24 -2.4974747474747474 3 25 -2.8611111111111107 3 26 -2.896464646464646 3 27 -3.2361111111111116 3 28 -3.258838383838384 3 29 -2.691919191919192 3 30 -3.041666666666667 3 31 -2.976010101010101 3 32 -3.1401515151515147 3 33 -2.5315656565656557 3 34 -3.066919191919192 3 35 -2.7348484848484844 3 36 -2.621212121212121 3 37 -2.5 3 38 -2.3219696969696972 3 39 -2.4116161616161618 3 40 -2.3686868686868685 5 1 -10.267676767676766 5 2 -7.154040404040403 5 3 -4.255050505050505 5 4 -3.8661616161616177 5 5 -4.965909090909092 5 6 -4.3345959595959584 5 7 -4.521464646464647 5 8 -4.88510101010101 5 9 -4.67550505050505 5 10 -4.371212121212121 5 11 -4.479797979797979 5 12 -4.159090909090908 5 13 -4.296717171717171 5 14 -4.380050505050505 5 15 -4.44949494949495 5 16 -3.342171717171718 5 17 -3.05429292929293 5 18 -2.6704545454545467 5 19 -3.0189393939393945 5 20 -3.037878787878788 5 21 -3.188131313131314 5 22 -3.0277777777777777 5 23 -3.128787878787879 5 24 -2.4974747474747474 5 25 -2.8611111111111107 5 26 -2.896464646464646 5 27 -3.2361111111111116 5 28 -3.258838383838384 5 29 -2.691919191919192 5 30 -3.041666666666667 5 31 -2.976010101010101 5 32 -3.1401515151515147 5 33 -2.5315656565656557 5 34 -3.066919191919192 5 35 -2.7348484848484844 5 36 -2.621212121212121 5 37 -2.5 5 38 -2.3219696969696972 5 39 -2.4116161616161618 5 40 -2.3686868686868685 8 1 -4.267676767676766 8 2 -4.82070707070707 8 3 1.0782828282828287 8 4 -1.8661616161616177 8 5 0.034090909090908283 8 6 -3.3345959595959584 8 7 -0.5214646464646471 8 8 -1.2184343434343434 8 9 -3.008838383838383 8 10 1.628787878787879 8 11 4.186868686868687 8 12 4.507575757575758 8 13 3.0366161616161627 8 14 -2.380050505050505 8 15 4.883838383838384 8 16 -1.342171717171718 8 17 0.612373737373737 8 18 2.3295454545454533 8 19 -2.6856060606060614 8 20 1.628787878787878 8 21 -0.5214646464646471 8 22 3.3055555555555562 8 23 -0.7954545454545459 8 24 -1.8308080808080804 8 25 2.1388888888888893 8 26 2.77020202020202 8 27 0.43055555555555536 8 28 2.407828282828282 8 29 0.641414141414141 8 30 -1.041666666666667 8 31 3.690656565656565 8 32 5.859848484848485 8 33 5.468434343434344 8 34 3.933080808080808 8 35 5.265151515151516 8 36 5.712121212121213 8 37 8.166666666666666 8 38 8.344696969696969 8 39 8.255050505050505 8 40 5.964646464646465 9 1 6.482323232323234 9 2 5.095959595959597 9 3 10.744949494949495 9 4 2.3838383838383823 9 5 2.5340909090909083 9 6 -1.8345959595959584 9 7 3.728535353535353 9 8 2.3648989898989896 9 9 6.82449494949495 9 10 5.878787878787879 9 11 4.770202020202021 9 12 5.090909090909092 9 13 1.2032828282828287 9 14 -1.3800505050505052 9 15 7.55050505050505 9 16 4.407828282828282 9 17 4.69570707070707 9 18 6.079545454545453 9 19 1.7310606060606055 9 20 8.212121212121211 9 21 6.061868686868686 9 22 5.972222222222222 9 23 7.371212121212121 9 24 4.002525252525253 9 25 4.638888888888889 9 26 9.353535353535353 9 27 9.01388888888889 9 28 10.491161616161616 9 29 9.558080808080808 9 30 8.958333333333332 9 31 10.7739898989899 9 32 11.609848484848484 9 33 11.718434343434344 9 34 8.433080808080808 9 35 10.765151515151516 9 36 9.378787878787879 9 37 11.0 9 38 10.178030303030303 9 39 9.338383838383837 9 40 10.131313131313131 13 1 -10.267676767676766 13 2 -7.154040404040403 13 3 -4.255050505050505 13 4 -3.8661616161616177 13 5 -4.965909090909092 13 6 -4.3345959595959584 13 7 -4.521464646464647 13 8 -4.88510101010101 13 9 -4.67550505050505 13 10 -4.371212121212121 13 11 -4.479797979797979 13 12 -4.159090909090908 13 13 -4.296717171717171 13 14 -4.380050505050505 13 15 -4.44949494949495 13 16 -3.342171717171718 13 17 -3.05429292929293 13 18 -2.6704545454545467 13 19 -3.0189393939393945 13 20 -3.037878787878788 13 21 -3.188131313131314 13 22 -3.0277777777777777 13 23 -3.128787878787879 13 24 -2.4974747474747474 13 25 -2.8611111111111107 13 26 -2.896464646464646 13 27 -3.2361111111111116 13 28 -3.258838383838384 13 29 -2.691919191919192 13 30 -3.041666666666667 13 31 -2.976010101010101 13 32 -3.1401515151515147 13 33 -2.5315656565656557 13 34 -3.066919191919192 13 35 -2.7348484848484844 13 36 -2.621212121212121 13 37 -2.5 13 38 -2.3219696969696972 13 39 -2.4116161616161618 13 40 -2.3686868686868685 16 1 20.982323232323232 16 2 18.5959595959596 16 3 23.744949494949495 16 4 21.13383838383838 16 5 13.784090909090908 16 6 22.41540404040404 16 7 23.728535353535353 16 8 26.36489898989899 16 9 25.324494949494948 16 10 20.12878787878788 16 11 25.52020202020202 16 12 20.590909090909093 16 13 22.95328282828283 16 14 23.119949494949495 16 15 13.05050505050505 16 16 15.907828282828282 16 17 21.44570707070707 16 18 16.079545454545453 16 19 9.731060606060606 16 20 13.462121212121211 16 21 14.561868686868685 16 22 13.722222222222221 16 23 16.37121212121212 16 24 8.752525252525253 16 25 8.88888888888889 16 26 10.853535353535353 16 27 8.51388888888889 16 28 0.7411616161616159 16 29 8.058080808080808 16 30 6.208333333333333 16 31 6.773989898989899 16 32 9.609848484848484 16 33 9.968434343434344 16 34 9.433080808080808 16 35 6.015151515151516 16 36 10.878787878787879 16 37 4.25 16 38 8.428030303030303 16 39 7.838383838383838 16 40 8.131313131313131 18 1 -1.6010101010101003 18 2 -1.8207070707070692 18 3 -0.5883838383838382 18 4 -0.8661616161616177 18 5 -1.2992424242424248 18 6 -3.3345959595959584 18 7 -3.521464646464647 18 8 -3.8851010101010104 18 9 -3.67550505050505 18 10 2.628787878787879 18 11 -0.1464646464646453 18 12 -1.4924242424242413 18 13 3.7032828282828287 18 14 -0.04671717171717127 18 15 5.217171717171716 18 16 3.657828282828282 18 17 0.94570707070707 18 18 -1.6704545454545467 18 19 -1.0189393939393945 18 20 -2.037878787878788 18 21 4.811868686868686 18 22 4.972222222222222 18 23 3.204545454545455 18 24 -1.4974747474747474 18 25 3.472222222222223 18 26 6.77020202020202 18 27 7.097222222222222 18 28 3.07449494949495 18 29 6.308080808080808 18 30 7.291666666666667 18 31 5.357323232323233 18 32 3.8598484848484853 18 33 5.801767676767678 18 34 6.599747474747474 18 35 -2.7348484848484844 18 36 -2.621212121212121 18 37 -2.5 18 38 -2.3219696969696972 18 39 -2.4116161616161618 18 40 -2.3686868686868685 19 1 15.982323232323234 19 2 11.595959595959597 19 3 7.244949494949495 19 4 5.383838383838382 19 5 15.034090909090908 19 6 6.9154040404040416 19 7 9.478535353535353 19 8 12.86489898989899 19 9 12.57449494949495 19 10 17.12878787878788 19 11 19.27020202020202 19 12 12.340909090909092 19 13 18.45328282828283 19 14 11.369949494949495 19 15 22.300505050505052 19 16 6.907828282828282 19 17 11.94570707070707 19 18 14.829545454545453 19 19 18.481060606060606 19 20 13.962121212121211 19 21 18.061868686868685 19 22 21.47222222222222 19 23 19.62121212121212 19 24 15.002525252525253 19 25 21.13888888888889 19 26 13.603535353535353 19 27 14.01388888888889 19 28 20.741161616161616 19 29 16.30808080808081 19 30 22.458333333333332 19 31 21.2739898989899 19 32 9.859848484848484 19 33 23.718434343434346 19 34 18.93308080808081 19 35 17.765151515151516 19 36 18.12878787878788 19 37 17.75 19 38 15.428030303030303 19 39 18.088383838383837 19 40 14.631313131313131 20 1 16.065656565656568 20 2 17.179292929292927 20 3 0.07828282828282873 20 4 5.800505050505048 20 5 10.700757575757576 20 6 -1.6679292929292915 20 7 8.478535353535353 20 8 7.448232323232322 20 9 7.991161616161618 20 10 4.628787878787879 20 11 6.186868686868687 20 12 5.174242424242426 20 13 5.703282828282829 20 14 7.619949494949495 20 15 5.217171717171716 20 16 6.324494949494948 20 17 5.612373737373736 20 18 0.9962121212121202 20 19 2.3143939393939394 20 20 6.962121212121212 20 21 7.14520202020202 20 22 6.972222222222222 20 23 7.871212121212121 20 24 7.8358585858585865 20 25 7.805555555555555 20 26 6.77020202020202 20 27 12.76388888888889 20 28 14.741161616161616 20 29 7.974747474747474 20 30 9.291666666666664 20 31 13.357323232323232 20 32 10.526515151515152 20 33 15.801767676767676 20 34 16.26641414141414 20 35 10.265151515151516 20 36 16.71212121212121 20 37 7.166666666666666 20 38 13.011363636363635 20 39 2.921717171717172 20 40 7.297979797979798 25 1 14.732323232323234 25 2 17.8459595959596 25 3 6.744949494949495 25 4 5.133838383838382 25 5 10.034090909090908 25 6 9.665404040404042 25 7 6.478535353535353 25 8 15.11489898989899 25 9 16.324494949494948 25 10 13.628787878787879 25 11 26.52020202020202 25 12 23.840909090909093 25 13 23.70328282828283 25 14 26.619949494949495 25 15 20.550505050505052 25 16 25.657828282828284 25 17 19.94570707070707 25 18 19.329545454545453 25 19 20.981060606060606 25 20 25.96212121212121 25 21 23.811868686868685 25 22 4.972222222222222 25 23 23.87121212121212 25 24 18.502525252525253 25 25 23.13888888888889 25 26 15.103535353535353 25 27 22.76388888888889 25 28 23.741161616161616 25 29 5.308080808080808 25 30 20.958333333333332 25 31 8.0239898989899 25 32 23.859848484848484 25 33 14.468434343434344 25 34 19.93308080808081 25 35 17.265151515151516 25 36 26.37878787878788 25 37 26.5 25 38 21.678030303030305 25 39 26.588383838383837 25 40 20.63131313131313 26 1 -10.267676767676766 26 2 -7.154040404040403 26 3 -4.255050505050505 26 4 -3.8661616161616177 26 5 -4.965909090909092 26 6 -4.3345959595959584 26 7 -4.521464646464647 26 8 -4.88510101010101 26 9 -4.67550505050505 26 10 -4.371212121212121 26 11 -4.479797979797979 26 12 -4.159090909090908 26 13 -4.296717171717171 26 14 -4.380050505050505 26 15 -4.44949494949495 26 16 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14 -4.380050505050505 113 15 -4.44949494949495 113 16 -3.342171717171718 113 17 -3.05429292929293 113 18 -2.6704545454545467 113 19 -3.0189393939393945 113 20 -3.037878787878788 113 21 -3.188131313131314 113 22 -3.0277777777777777 113 23 -3.128787878787879 113 24 -2.4974747474747474 113 25 -2.8611111111111107 113 26 -2.896464646464646 113 27 -3.2361111111111116 113 28 -3.258838383838384 113 29 -2.691919191919192 113 30 -3.041666666666667 113 31 -2.976010101010101 113 32 -3.1401515151515147 113 33 -2.5315656565656557 113 34 -3.066919191919192 113 35 -2.7348484848484844 113 36 -2.621212121212121 113 37 -2.5 113 38 -2.3219696969696972 113 39 -2.4116161616161618 113 40 -2.3686868686868685 114 1 1.2323232323232336 114 2 -0.6540404040404031 114 3 2.244949494949495 114 4 -1.8661616161616177 114 5 2.5340909090909083 114 6 -1.3345959595959584 114 7 4.978535353535353 114 8 6.61489898989899 114 9 3.32449494949495 114 10 3.128787878787879 114 11 0.5202020202020208 114 12 4.840909090909092 114 13 0.20328282828282873 114 14 7.119949494949495 114 15 2.05050505050505 114 16 -0.342171717171718 114 17 3.44570707070707 114 18 5.329545454545453 114 19 1.9810606060606055 114 20 4.962121212121212 114 21 5.811868686868686 114 22 8.472222222222221 114 23 0.8712121212121211 114 24 3.0025252525252526 114 25 1.6388888888888893 114 26 3.603535353535354 114 27 0.7638888888888884 114 28 4.741161616161616 114 29 7.308080808080808 114 30 4.958333333333333 114 31 12.5239898989899 114 32 12.359848484848484 114 33 12.968434343434344 114 34 12.433080808080808 114 35 12.765151515151516 114 36 -2.621212121212121 114 37 -2.5 114 38 -2.3219696969696972 114 39 -2.4116161616161618 114 40 -2.3686868686868685 116 1 11.732323232323234 116 2 18.8459595959596 116 3 -4.255050505050505 116 4 -3.8661616161616177 116 5 -4.965909090909092 116 6 -4.3345959595959584 116 7 -4.521464646464647 116 8 -4.88510101010101 116 9 -4.67550505050505 116 10 -4.371212121212121 116 11 -4.479797979797979 116 12 -4.159090909090908 116 13 -4.296717171717171 116 14 -4.380050505050505 116 15 -4.44949494949495 116 16 -3.342171717171718 116 17 -3.05429292929293 116 18 -2.6704545454545467 116 19 -3.0189393939393945 116 20 -3.037878787878788 116 21 -3.188131313131314 116 22 -3.0277777777777777 116 23 -3.128787878787879 116 24 -2.4974747474747474 116 25 -2.8611111111111107 116 26 -2.896464646464646 116 27 -3.2361111111111116 116 28 -3.258838383838384 116 29 -2.691919191919192 116 30 -3.041666666666667 116 31 -2.976010101010101 116 32 -3.1401515151515147 116 33 -2.5315656565656557 116 34 -3.066919191919192 116 35 -2.7348484848484844 116 36 -2.621212121212121 116 37 -2.5 116 38 -2.3219696969696972 116 39 -2.4116161616161618 116 40 -2.3686868686868685 117 1 11.732323232323234 117 2 9.845959595959597 117 3 4.744949494949495 117 4 3.1338383838383823 117 5 7.034090909090908 117 6 8.665404040404042 117 7 1.478535353535353 117 8 11.11489898989899 117 9 14.32449494949495 117 10 -1.371212121212121 117 11 4.520202020202021 117 12 10.840909090909092 117 13 1.7032828282828287 117 14 -0.3800505050505052 117 15 2.55050505050505 117 16 2.657828282828282 117 17 9.94570707070707 117 18 9.329545454545453 117 19 11.981060606060606 117 20 15.962121212121211 117 21 14.811868686868685 117 22 25.97222222222222 117 23 25.87121212121212 117 24 26.502525252525253 117 25 23.13888888888889 117 26 29.103535353535353 117 27 22.76388888888889 117 28 28.741161616161616 117 29 19.30808080808081 117 30 25.958333333333332 117 31 -2.976010101010101 117 32 -3.1401515151515147 117 33 -2.5315656565656557 117 34 -3.066919191919192 117 35 -2.7348484848484844 117 36 -2.621212121212121 117 37 -2.5 117 38 -2.3219696969696972 117 39 -2.4116161616161618 117 40 -2.3686868686868685 119 1 17.232323232323232 119 2 2.345959595959597 119 3 3.744949494949495 119 4 2.6338383838383823 119 5 1.0340909090909083 119 6 6.1654040404040416 119 7 7.478535353535353 119 8 3.6148989898989896 119 9 1.8244949494949498 119 10 7.128787878787879 119 11 5.020202020202021 119 12 -1.1590909090909083 119 13 3.7032828282828287 119 14 9.119949494949495 119 15 9.05050505050505 119 16 -3.342171717171718 119 17 -3.05429292929293 119 18 -2.6704545454545467 119 19 -3.0189393939393945 119 20 -3.037878787878788 119 21 -3.188131313131314 119 22 -3.0277777777777777 119 23 -3.128787878787879 119 24 -2.4974747474747474 119 25 -2.8611111111111107 119 26 -2.896464646464646 119 27 -3.2361111111111116 119 28 -3.258838383838384 119 29 -2.691919191919192 119 30 -3.041666666666667 119 31 -2.976010101010101 119 32 -3.1401515151515147 119 33 -2.5315656565656557 119 34 -3.066919191919192 119 35 -2.7348484848484844 119 36 -2.621212121212121 119 37 -2.5 119 38 -2.3219696969696972 119 39 -2.4116161616161618 119 40 -2.3686868686868685 >>END_MODULE >>Per sequence quality scores fail #Quality Count 4 752.0 5 51.0 6 20.0 7 24.0 8 10.0 9 16.0 10 36.0 11 36.0 12 19.0 13 12.0 14 19.0 15 11.0 16 6.0 17 5.0 18 15.0 19 20.0 20 28.0 21 19.0 22 39.0 23 24.0 24 15.0 25 18.0 26 9.0 27 7.0 28 5.0 29 2.0 30 2.0 31 1.0 32 2.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 0.0 0.0 0.0 100.0 2 0.0 0.0 0.0 100.0 3 0.0 0.0 100.0 0.0 4 0.0 100.0 0.0 0.0 5 100.0 0.0 0.0 0.0 6 0.0 100.0 0.0 0.0 7 99.26350245499181 0.24549918166939444 0.32733224222585927 0.16366612111292964 8 97.62878168438267 0.7358953393295176 0.49059689288634506 1.1447260834014716 9 98.03439803439802 0.819000819000819 0.5733005733005733 0.5733005733005733 10 62.76595744680851 19.148936170212767 10.556464811783961 7.528641571194762 11 39.820114472608346 21.504497138184792 20.768601798855276 17.906786590351594 12 39.00245298446443 21.668029435813573 20.768601798855276 18.560915780866722 13 23.404255319148938 20.54009819967267 24.79541734860884 31.260229132569556 14 18.985270049099835 31.50572831423895 31.342062193126026 18.16693944353519 15 32.379394930498776 18.397383483237938 31.725265739983648 17.497955846279638 16 32.54292722812755 25.42927228127555 25.919869174161896 16.107931316434996 17 27.22812755519215 23.548650858544562 34.341782502044154 14.881439084219133 18 30.907604251839736 22.567457072771873 24.36631234668847 22.158626328699917 19 24.57002457002457 30.13923013923014 23.832923832923832 21.457821457821456 20 28.781684382665578 24.856909239574815 32.86999182338512 13.49141455437449 21 25.020441537203595 27.14636140637776 24.693376941946035 23.13982011447261 22 22.48569092395748 24.448078495502862 35.568274734260015 17.497955846279638 23 24.1210139002453 22.158626328699917 34.66884709730172 19.051512673753066 24 27.88225674570728 26.410466067048244 25.59280457890433 20.114472608340147 25 24.304418985270047 25.20458265139116 26.268412438625205 24.222585924713584 26 27.95081967213115 24.508196721311474 28.196721311475407 19.34426229508197 27 19.556285949055052 28.430566967953986 26.129827444535742 25.883319638455216 28 21.685761047463174 28.55973813420622 25.450081833060555 24.304418985270047 29 30.02461033634126 27.973748974569318 21.57506152584085 20.426579163248565 30 25.265739983646768 25.10220768601799 31.479967293540472 18.152085036794766 31 31.234668847097304 24.28454619787408 27.88225674570728 16.59852820932134 32 31.342062193126026 26.104746317512273 25.040916530278235 17.51227495908347 33 33.60588716271464 23.30335241210139 26.165167620605068 16.925592804578905 34 23.30335241210139 22.24039247751431 37.77596075224857 16.68029435813573 35 20.27800490596893 30.08994276369583 35.15944399018806 14.472608340147177 36 20.344544708777686 33.305988515176374 28.219852337981955 18.12961443806399 37 26.492232215862632 30.662305805396567 26.98282910874898 15.862632869991824 38 25.020441537203595 30.98937040065413 24.775143090760427 19.215044971381847 39 28.454619787408014 29.435813573180702 22.07686017988553 20.032706459525755 40 23.221586263287 24.856909239574815 28.454619787408014 23.46688470973017 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.5 15 1.0 16 1.0 17 1.0 18 1.0 19 3.0 20 5.0 21 5.5 22 6.0 23 6.0 24 4.5 25 3.0 26 5.0 27 7.0 28 7.0 29 12.5 30 18.0 31 19.5 32 21.0 33 21.0 34 26.0 35 31.0 36 40.5 37 50.0 38 50.0 39 53.0 40 56.0 41 58.5 42 61.0 43 61.0 44 80.5 45 100.0 46 98.5 47 97.0 48 97.0 49 93.0 50 89.0 51 100.5 52 112.0 53 112.0 54 115.0 55 118.0 56 112.0 57 106.0 58 106.0 59 122.5 60 139.0 61 126.5 62 114.0 63 114.0 64 82.0 65 50.0 66 36.5 67 23.0 68 23.0 69 16.0 70 9.0 71 6.0 72 3.0 73 3.0 74 3.0 75 3.0 76 1.5 77 0.0 78 0.0 79 0.0 80 0.0 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.08176614881439084 8 0.0 9 0.1635322976287817 10 0.08176614881439084 11 0.0 12 0.0 13 0.08176614881439084 14 0.08176614881439084 15 0.0 16 0.0 17 0.0 18 0.0 19 0.1635322976287817 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.08176614881439084 26 0.24529844644317253 27 0.49059689288634506 28 0.08176614881439084 29 0.3270645952575634 30 0.0 31 0.0 32 0.08176614881439084 33 0.0 34 0.0 35 0.0 36 0.3270645952575634 37 0.0 38 0.0 39 0.0 40 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 40 1223.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 89.12510220768601 #Duplication Level Percentage of deduplicated Percentage of total 1 94.40366972477065 84.13736713000817 2 4.3119266055045875 7.686017988552739 3 0.3669724770642202 0.9811937857726901 4 0.1834862385321101 0.6541291905151267 5 0.1834862385321101 0.8176614881439084 6 0.1834862385321101 0.9811937857726901 7 0.0 0.0 8 0.0 0.0 9 0.0 0.0 >10 0.3669724770642202 4.742436631234669 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source CCTAGAGGGTCACAGATCCTCTTCTGAGATGAGTTTTTGT 21 1.7170891251022076 No Hit CCTAGAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGC 14 1.1447260834014716 No Hit CCTAGAGGGTCACAGATCCTCTTCTGAAGGAGATTGGGCG 12 0.9811937857726901 No Hit CCTAGAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAG 11 0.8994276369582993 No Hit CCTAGAGGGTCAAAGATCCTCTTCTGAGATGAGTTTTTGT 6 0.49059689288634506 No Hit CCTAGAGGGGTGGGTTGGTGAGATGGCTCAGTGGGTAAGA 6 0.49059689288634506 No Hit CCTAGAGGGACTAATCCTAGCCCTAGCCCTACACAAATAT 5 0.4088307440719542 No Hit CCTAGAGGGGGGCAGGGATTGGGACTGTTTTTGGGAGTCT 5 0.4088307440719542 No Hit CCTAGAGGGGGGGCTGGAGAGATGGCTCAGTGGTTAAGAG 4 0.3270645952575634 No Hit CCTAGAGGGGGGAAGGGATTGGGACTGTTTTTGGGAGTCT 4 0.3270645952575634 No Hit CCTAGAGGGAAGAATGTTATGTTTACTCCCACTTCGCCAT 3 0.24529844644317253 No Hit CCTAGAGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCA 3 0.24529844644317253 No Hit CCTAGAGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGC 3 0.24529844644317253 No Hit CCTAGAGGGTCAAAGATCCTCTTCTGAAGGAGATTGGGCG 3 0.24529844644317253 No Hit CCTAGAGGGGGGGCTGAAGAGATGGCTCAGCGGTTAAGAG 2 0.1635322976287817 No Hit CCTAGAGGGGTTTGCCAAACTTCTTGATCTTGGGAGCCAG 2 0.1635322976287817 No Hit CCTAGAGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGA 2 0.1635322976287817 No Hit CCTAGAGGGCGTCTCATGGCCGCTAGTAGATCCCTGGTGC 2 0.1635322976287817 No Hit CCTAGAGGGGCCCTTTCTTGGTGTGTGGGGGATCTATGGC 2 0.1635322976287817 No Hit CCTAGAGGGGGGTTAGAGAGATGGCTTAGTGGTTAAGAGC 2 0.1635322976287817 No Hit CCTAGAGGGATGCCACAACTAGATACATCAACATGATTTA 2 0.1635322976287817 No Hit CCTAGAGGGGGGGCTGGCGACACGGCTCAGTGGTTAAGAG 2 0.1635322976287817 No Hit CCTAGAGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCG 2 0.1635322976287817 No Hit CCTAGAGGGTCACAGATCCTCTTATGAGATGAGTTTTTGT 2 0.1635322976287817 No Hit CCTAGAGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAACG 2 0.1635322976287817 No Hit CCTAGAGGGCTCTATGAAGACCATGGAGACACCCTTTCCC 2 0.1635322976287817 No Hit CCTAGAGGGTACGAATTCTAAAGCAATCATTTTTGTGTGG 2 0.1635322976287817 No Hit CCTAGAGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTT 2 0.1635322976287817 No Hit CCTAGAGGGGCCTCTTCTGAGATGAGTTTTTGTTCAATCT 2 0.1635322976287817 No Hit CCTAGAGGGGTGTAGTAACTCTCTAAAATGTCTGGAGATG 2 0.1635322976287817 No Hit CCTAGAGGGAATGAGTTGGGGGGTAACCCCTAATCTGTAT 2 0.1635322976287817 No Hit CCTAGAGGGGACTTGCTGTTGTCCAGAATTCACTTACCCC 2 0.1635322976287817 No Hit CCTAGAGGGTCACGGATCCTCTTCTGAGATGAGTTTTTGT 2 0.1635322976287817 No Hit CCTAGAGGGAGGGCTGGTGAGATGGCTCAGCGGTTAAGAT 2 0.1635322976287817 No Hit CCTAGAGGGGAGCCTTAGGTTTTATTTTCTTATTTACAGT 2 0.1635322976287817 No Hit CCTAGAGGGGGCCTTTCAGGAACACCACGACGCTACTCAG 2 0.1635322976287817 No Hit CCTAGAGGGTCCCAGATCCTCTTCTGAAGGAGATTGGGCG 2 0.1635322976287817 No Hit CCTAGAGGGGTGTCATTTTATATATGTTCCTAATGCTTAC 2 0.1635322976287817 No Hit CCTAGAGGGGTCACAGATCCTCTTCTGAAGGAGATTGGGC 2 0.1635322976287817 No Hit CCTAGAGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAA 2 0.1635322976287817 No Hit CCTAGAGGGATGATTGAAAGCATAGAAGCCAATGTAGAAA 2 0.1635322976287817 No Hit CCTAGAGGGGGGAATTAAATGTGCAATTAAATGCTCTGTG 2 0.1635322976287817 No Hit CCTAGAGGGATTTATGGGATTTCTTCCCAGTATGGATCTT 2 0.1635322976287817 No Hit CCTAGAGGGTACCTGGTTGATCCTGCCAGTAGCATATGCT 2 0.1635322976287817 No Hit CCTAGAGGGGGTCTAACCGGAATTGTTTTATCCAACTCAT 2 0.1635322976287817 No Hit CCTAGAGGGGCCGGTAGTGGTGGCGCACGCCGGTAGGATT 2 0.1635322976287817 No Hit CCTAGAGGGGGGGCTGGTGAGATGGCTCAGTGAGTAAGAG 2 0.1635322976287817 No Hit CCTAGAGGGTCACAGATCCTCTTATGAAGGAGATTGGGCG 2 0.1635322976287817 No Hit CCTAGAGGGCGTCTGTGGAGCAGCTCCTGTCTCCCAACTA 2 0.1635322976287817 No Hit CCTAGAGGGGGGATGGTGAGATGGCTCAGTGGGTAAGAGC 2 0.1635322976287817 No Hit CCTAGAGGGGGTCATGGTGGGCCTCTACTTGCTCATCTAC 2 0.1635322976287817 No Hit CCTAGAGGGGTGTTCTTGTTCCTGTCAGCAGCACATTGCG 2 0.1635322976287817 No Hit CCTAGAGGGGGTGGTCCCTGTGTTAATCGGGGCTGAGGGT 2 0.1635322976287817 No Hit CCTAGAGGGGTCAGTGACGAGAGGCTTTGTCCGGTTCGGC 2 0.1635322976287817 No Hit CCTAGAGGGGAAGACCCGCCTTAATTGTGGGCGGAGCCGT 2 0.1635322976287817 No Hit CCTAGAGGGGCGCTCTGAGCTGTTTTGTTGAAGTAGAAAA 2 0.1635322976287817 No Hit CCTAGAGGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGC 2 0.1635322976287817 No Hit CCTAGAGGGGGGCTGCCACAGTGACCTTGACCTAGCGAGG 2 0.1635322976287817 No Hit CCTAGAGGGTCTGCTTCTTATATATTATTACAGTTTGCAC 2 0.1635322976287817 No Hit CCTAGAGGGGGCTGGTGAAATGGCTCAGTGGGTAAGAGCA 2 0.1635322976287817 No Hit CCTAGAGGGGCAATAATGTCTATTGGCTTTCTAGGCTTTA 2 0.1635322976287817 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0 0.0 0.0 0.0 0.0 11 0.0 0.0 0.0 0.0 0.0 12 0.0 0.0 0.0 0.0 0.0 13 0.0 0.0 0.0 0.0 0.0 14 0.0 0.0 0.0 0.0 0.0 15 0.0 0.0 0.0 0.0 0.0 16 0.0 0.0 0.0 0.0 0.0 17 0.0 0.0 0.0 0.0 0.0 18 0.0 0.0 0.0 0.0 0.0 19 0.0 0.0 0.0 0.0 0.0 20 0.0 0.0 0.0 0.0 0.0 21 0.0 0.0 0.0 0.0 0.0 22 0.0 0.0 0.0 0.0 0.0 23 0.0 0.0 0.0 0.0 0.0 24 0.0 0.0 0.0 0.0 0.0 25 0.0 0.0 0.0 0.0 0.0 26 0.0 0.0 0.0 0.0 0.0 27 0.0 0.0 0.0 0.0 0.0 28 0.0 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TAGAGGG 120 0.0 34.0 3 CTAGAGG 120 0.0 34.0 2 CCTAGAG 120 0.0 34.0 1 AGAGGGG 70 0.0 33.999996 4 AGAGGGC 20 0.0018237206 33.999996 4 GAGGGGG 20 0.0018237206 33.999996 5 GAGGGGA 20 0.0018237206 33.999996 5 >>END_MODULE