FastQCFastQC Report
Fri 17 Jun 2016
SRR1526150_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526150_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16484
Sequences flagged as poor quality0
Sequence length56
%GC51

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTCTGGGGTCACAGATCCTCTTCTGAGATGAGTTTTTGTTCAATCCGCCCAATCT3462.0990050958505218No Hit
CTTCTGGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAG3432.080805629701529No Hit
CTTCTGGGGTCACAGATCCTCTTCTGAAGGAGATTGGGCGGATTGAACAAAAACTC2281.383159427323465No Hit
CTTCTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC1300.7886435331230284No Hit
CTTCTGGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTCGTCTCAAAGATTAAG1030.6248483377820917No Hit
CTTCTGGGGTCACAGATCCTCTTCTGAAGGAGATTGGGCGCATTGAACAAAAACTC750.45498665372482405No Hit
CTTCTGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTC730.4428536762921621No Hit
CTTCTGGGGTCACAGATCCTCTTCTGAGATGAGTTTTTGTCCAATCCGCCCAATCT520.31545741324921134No Hit
CTTCTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCCCCCGACTGCTCTTCC470.2851249696675564No Hit
CTTCTGGGGTCACAGATCCTCTTCTGAGATGAGTTTTTGTTCAATCCGCCCAAAAA440.2669255035185635No Hit
CTTCTGGGGTACCTGGTTGATCCTGCCAGTAGCATATGCCTGTCTCAAAGATTAAG440.2669255035185635No Hit
CTTCTGGGTCACAGATCCTCTTCTGAGATGAGTTTTTGTTCAATCCGCCCAATCTC430.26085901480223245No Hit
CTTCTGGGGACTAATCCTAGCCCTAGCCCTACACAAATATAATTATACTATTATAT410.2487260373695705No Hit
CTTCTGGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGC410.2487260373695705No Hit
CTTCTGGGGTCACAGATCCTCTTCTGAAGGAGATTGGGCCGATTGAACAAAAACTC390.23659305993690852No Hit
CTTCTGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA380.23052657122057751No Hit
CTTCTGGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCCAAGATTAAG330.20019412763892258No Hit
CTTCTGGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTT260.15772870662460567No Hit
CTTCTGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA260.15772870662460567No Hit
CTTCTGGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCCACGATTAAG250.1516622179082747No Hit
CTTCTGGGGGGGCGCTGACCCCCCTTCCCGGGGGGGGATGCGTGCATTTATCAGAT230.13952924047561271No Hit
CTTCTGGGGTCACAGATCCTCTTCTGAGATGAGTTTTTGCTCAATCCGCCCAATCT230.13952924047561271No Hit
CTTCTGGGGACTAATCCTAGCCCTAGCCCTACACAAATATCATTATACTATTATAT230.13952924047561271No Hit
CTTCTGGGGGGGCTGGAGAGATGGCTCAGTGGTTAAGAGCACTGACTGCTCTTCCA230.13952924047561271No Hit
CTTCTGGGGTACCTGGTTGATCCTGCCAGTAGCATATGCCGTCTTCTGCTTGGATC220.13346275175928174No Hit
CTTCTGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACAAAAAAAAAAAAAAA220.13346275175928174No Hit
CTTCTGGGGTCACAGATCCTCTTCTGAAGGAGATTGGGCGGATTGACCAAAAACTC190.11526328561028876No Hit
CTTCTGGGGGCAAGCTCCGTTGGGCGCCTTGGATCCATTTCCATCTGGTTCTGCTG180.10919679689395778No Hit
CTTCTGGGGGATCCCCTCGACCACTCTTTTGGCGCTTCGCTGTCGACAGTGCGCTT170.10313030817762679No Hit
CTTCTGGGGTAAGGGAGAATCTTGCCTGGCTCTCTTCTCTTGGGCCTCTAAGAAAG170.10313030817762679No Hit
CTTCTGGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA170.10313030817762679No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCAATCC150.00770043150.9283541
TGTCTCC203.708414E-450.9283541
CGTCTCA203.708414E-450.9283541
CCCTGAC150.0078927550.61300340
GACCACC150.0078927550.61300338
AGCCCTG150.0078927550.61300338
GCCCTGA150.0078927550.61300339
GACCAAA150.00799023850.4567945
TTGTCCA150.0080886250.3015437
TAAGACC150.0080886250.3015435
AGCAGTG309.4494317E-750.3015411
AAGACCA150.0080886250.3015436
TAGAGCG150.0080886250.3015434
GTTGGGG150.0080886250.3015413
AGACCAC150.0080886250.3015437
AAGCAGT354.6446075E-850.3015410
TATGCTC203.9436133E-450.30153735
AGAGCCC203.9436133E-450.30153736
GGCTCTT203.9436133E-450.3015379
ATGCTCG203.9436133E-450.30153736
Produced by FastQC (version 0.11.5)