##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1526150_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 16484 Sequences flagged as poor quality 0 Sequence length 56 %GC 51 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 38.39389711235137 39.0 38.0 40.0 36.0 40.0 2 37.829349672409606 39.0 38.0 40.0 35.0 40.0 3 37.435816549381215 39.0 38.0 40.0 34.0 40.0 4 37.37393836447464 39.0 38.0 40.0 33.0 40.0 5 37.654452802717785 39.0 38.0 40.0 34.0 40.0 6 36.601310361562724 39.0 36.0 40.0 31.0 40.0 7 37.5615141955836 39.0 38.0 40.0 34.0 40.0 8 37.39923562242174 39.0 38.0 40.0 33.0 40.0 9 37.40481679204077 39.0 38.0 40.0 34.0 40.0 10 36.57710507158457 39.0 36.0 40.0 32.0 40.0 11 35.174532880368844 38.0 35.0 40.0 26.0 40.0 12 34.26431691337054 38.0 33.0 40.0 22.0 40.0 13 33.834324193157 38.0 32.0 40.0 20.0 40.0 14 34.42101431691337 38.0 33.0 40.0 23.0 40.0 15 33.02638922591604 38.0 31.0 39.0 17.0 40.0 16 32.770019412763894 38.0 30.0 39.0 18.0 40.0 17 31.63722397476341 36.0 27.0 39.0 15.0 40.0 18 31.171438971123514 36.0 26.0 39.0 14.0 40.0 19 32.38303809754914 37.0 29.0 39.0 17.0 40.0 20 33.01789614171317 37.0 30.0 39.0 20.0 40.0 21 33.274811938849794 38.0 31.0 39.0 19.0 40.0 22 32.484469788886194 38.0 30.0 39.0 16.0 40.0 23 33.272931327347735 38.0 31.0 40.0 19.0 40.0 24 32.6018563455472 38.0 30.0 39.0 17.0 40.0 25 33.13904392137831 38.0 31.0 39.0 19.0 40.0 26 33.39341179325407 38.0 32.0 39.0 19.0 40.0 27 33.72913127881582 38.0 33.0 39.0 20.0 40.0 28 33.759949041494785 38.0 33.0 39.0 22.0 40.0 29 33.175139529240475 38.0 32.0 39.0 18.0 40.0 30 32.94946614899296 38.0 31.0 39.0 17.0 40.0 31 33.679022082018925 38.0 33.0 39.0 20.0 40.0 32 33.56551807813637 38.0 33.0 39.0 19.0 40.0 33 33.324678476098036 38.0 33.0 39.0 19.0 40.0 34 33.08123028391167 37.0 33.0 39.0 18.0 40.0 35 33.25630914826498 38.0 33.0 39.0 18.0 40.0 36 32.305387041980104 36.0 31.0 39.0 13.0 40.0 37 32.10537490900267 36.0 31.0 39.0 11.0 40.0 38 31.370177141470517 36.0 30.0 38.0 4.0 40.0 39 30.237806357680174 35.0 27.0 38.0 4.0 40.0 40 27.977796651298227 34.0 19.0 38.0 4.0 40.0 41 26.10901480223247 31.0 14.0 38.0 4.0 40.0 42 27.015105556903663 32.0 20.0 37.0 4.0 39.0 43 27.3632613443339 33.0 20.0 38.0 4.0 40.0 44 26.473368114535308 31.0 17.0 38.0 4.0 40.0 45 27.373028391167193 33.0 21.0 38.0 4.0 40.0 46 25.30150448920165 30.0 16.0 36.0 4.0 39.0 47 24.386314001455958 29.0 10.0 36.0 4.0 39.0 48 25.048774569279303 29.0 15.0 36.0 4.0 39.0 49 24.063940791070127 28.0 9.0 36.0 4.0 39.0 50 24.77511526328561 29.0 12.0 36.0 4.0 39.0 51 24.997937393836448 30.0 4.0 36.0 4.0 39.0 52 25.019534093666586 30.0 4.0 36.0 4.0 39.0 53 24.829046347973794 30.0 4.0 36.0 4.0 39.0 54 24.455532637709293 30.0 4.0 36.0 4.0 39.0 55 24.102887648628972 30.0 4.0 36.0 4.0 39.0 56 23.488534336326133 29.0 4.0 36.0 4.0 39.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1 1 0.8320204823263566 1 2 1.3975090676502688 1 3 2.220199932241222 1 4 2.0107220185287105 1 5 1.6659310205487472 1 6 2.990227224046727 1 7 1.6380213615771453 1 8 1.6851205212022364 1 9 2.007191211647765 1 10 3.084327691607754 1 11 -0.509485729385112 1 12 -6.8544204839598954 1 13 -20.19032137253286 1 14 -4.4232416966041725 1 15 1.7574106803679648 1 16 -1.0017846913098758 1 17 -4.009719830984636 1 18 -4.2515367039741285 1 19 -0.2531908880325453 1 20 -0.39493785819101035 1 21 6.340663250674602 1 22 7.64179148438582 1 23 6.396368165504459 1 24 7.313074942715183 1 25 6.475469234869536 1 26 6.470770013630478 1 27 6.037624619217176 1 28 5.8608003860788145 1 29 5.460483377050437 1 30 5.827097936255637 1 31 4.688172746584186 1 32 4.383635852931953 1 33 4.608936725201854 1 34 4.61567080312382 1 35 4.741214794748689 1 36 4.952815098286081 1 37 4.997369779953864 1 38 6.009995216430685 1 39 7.368879515093067 1 40 -7.153551934057713 1 41 1.8294463776727028 1 42 1.7867462580727533 1 43 -6.698753390602782 1 44 -14.933314414080801 1 45 -1.0194964342882855 1 46 -3.5511732543080967 1 47 -11.036888881255981 1 48 -3.5445447981291025 1 49 -14.650208089684181 1 50 -3.0132271918998867 1 51 3.176057805658587 1 52 2.9599665983037546 1 53 3.396746829851935 1 54 2.567673459805796 1 55 3.879250171825859 1 56 7.847451844426317 2 1 1.2221719974778722 2 2 1.3369030070442065 2 3 2.326260538301831 2 4 2.237994745801437 2 5 1.8553249599426849 2 6 2.4750757088952113 2 7 1.490294088849872 2 8 1.5904235515052676 2 9 1.6890093934659447 2 10 2.6525095097895743 2 11 3.153393058493677 2 12 -0.9491174536568678 2 13 1.0710422638307762 2 14 3.95554618218371 2 15 5.139986437943723 2 16 4.233063793538605 2 17 6.664522593257789 2 18 4.631039053601626 2 19 6.004384869543209 2 20 4.044456081202927 2 21 6.639905674917031 2 22 6.9637611813555225 2 23 6.737277256413552 2 24 7.718377973018214 2 25 6.077741962142262 2 26 4.804103346963814 2 27 4.662624619217176 2 28 4.849436749715181 2 29 6.051392467959531 2 30 6.54300702716473 2 31 4.775293958705397 2 32 5.679090398386499 2 33 4.279391270656401 2 34 4.312640500093522 2 35 4.032881461415354 2 36 5.055087825558807 2 37 6.535248567832653 2 38 5.551661883097349 2 39 7.08478860600216 2 40 4.854023823518045 2 41 9.094597892824222 2 42 9.108715955042452 2 43 7.426246609397218 2 44 -4.683314414080801 2 45 6.916109626317777 2 46 6.429887351752509 2 47 4.8002323308652315 2 48 5.917576413992112 2 49 -0.4380868775629665 2 50 6.365560686887992 2 51 7.8578759874767705 2 52 7.331178719515876 2 53 7.908110466215572 2 54 7.552521944654281 2 55 8.924704717280406 2 56 7.820936692911168 3 1 1.5971719974778722 3 2 2.253569673710878 3 3 2.9095938716351597 3 4 2.821328079134773 3 5 2.480324959942685 3 6 3.558409042228547 3 7 2.3236274221832076 3 8 2.4237568848386033 3 9 2.605676060132609 3 10 3.6941761764562386 3 11 4.653393058493677 3 12 5.842549213009804 3 13 5.529375597164112 3 14 5.622212848850374 3 15 1.0983197712770583 3 16 6.8997304602052765 3 17 8.28952259325779 3 18 8.881039053601626 3 19 7.587718202876545 3 20 6.627789414536263 3 21 7.556572341583696 3 22 8.797094514688851 3 23 7.070610589746888 3 24 7.9267113063515495 3 25 7.702741962142262 3 26 7.345770013630478 3 27 6.662624619217176 3 28 6.432770083048517 3 29 6.468059134626195 3 30 7.251340360498066 3 31 3.4002939587053973 3 32 4.095757065053164 3 33 5.1127246039897365 3 34 4.520973833426851 3 35 2.3662147947486893 3 36 6.263421158892143 3 37 6.368581901165982 3 38 7.426661883097349 3 39 9.20978860600216 3 40 10.479023823518041 3 41 12.511264559490886 3 42 11.067049288375781 3 43 11.426246609397218 3 44 11.400018919252535 3 45 11.499442959651105 3 46 11.888220685085841 3 47 12.425232330865228 3 48 12.375909747325444 3 49 13.520246455770366 3 50 13.365560686887996 3 51 14.191209320810106 3 52 13.247845386182544 3 53 13.074777132882236 3 54 14.802521944654277 3 55 14.96637138394707 3 56 15.612603359577836 4 1 1.5523002026060766 4 2 2.2086978788390823 4 3 3.0313887434300355 4 4 2.776456284262977 4 5 2.4771198317375607 4 6 3.0007167345362404 4 7 2.2883710119267917 4 8 2.279526115607837 4 9 2.5736247780813315 4 10 3.171740279020341 4 11 5.0989058790064945 4 12 5.470754341214928 4 13 6.330657648446163 4 14 5.622212848850374 4 15 6.540627463584752 4 16 7.6625509730257875 4 17 8.504266183001373 4 18 9.339372386934961 4 19 8.337718202876545 4 20 6.531635568382416 4 21 7.277726187737542 4 22 8.351581694176033 4 23 6.5642003333366326 4 24 7.869018998659243 4 25 7.356588115988416 4 26 6.624616167476631 4 27 6.22993231152487 4 28 6.493667518945948 4 29 6.205238621805684 4 30 7.4949301040878105 4 31 5.233627292038733 4 32 6.390628859924959 4 33 5.654391270656401 4 34 6.363922551375573 4 35 5.510445563979456 4 36 6.795472440943421 4 37 7.067299849883931 4 38 8.279225985661451 4 39 9.152096298309853 4 40 -0.024181304687083127 4 41 12.011264559490886 4 42 11.30422877555527 4 43 10.839708147858758 4 44 11.912839432073046 4 45 10.746237831445981 4 46 13.83693863380379 4 47 15.008565664198564 4 48 12.193217439633138 4 49 13.741400301924212 4 50 14.762996584323894 4 51 15.28095291055369 4 52 14.222204360541514 4 53 14.302341235446338 4 54 14.501239893372226 4 55 12.732397024972713 4 56 13.836962333936807 5 1 1.6225343163184505 5 2 2.2354537316818934 5 3 2.971188074533714 5 4 2.5858208327579604 5 5 2.460397423710802 5 6 3.0040612161415936 5 7 2.430511480154223 5 8 2.0487568848386033 5 9 1.8846615673789913 5 10 3.5329442923982697 5 11 4.9015814642907785 5 12 4.922259357937335 5 13 6.272129220352518 5 14 5.665691109719937 5 15 7.348319771277058 5 16 7.55552756165455 5 17 8.602928390359235 5 18 9.230676734761047 5 19 7.555109507224373 5 20 7.3343111536667 5 21 7.344615819844563 5 22 8.201080021935233 5 23 6.835103343370076 5 24 8.263667828090682 5 25 6.92013326649009 5 26 6.079465665804392 5 27 6.472407227912825 5 28 5.898349793193439 5 29 7.203566381003007 5 30 6.831050505425598 5 31 4.994496857256124 5 32 5.842133876647367 5 33 5.284826053265096 5 34 5.4793071667601865 5 35 3.9803452295312987 5 36 5.5713921733848935 5 37 6.060610886673231 5 38 6.336082172952427 5 39 8.133701649480418 5 40 8.111270200329635 5 41 11.076481950795237 5 42 11.019947839100421 5 43 10.236029218092867 5 44 9.871033412006152 5 45 10.229515423419222 5 46 11.417206192332223 5 47 9.89987001202465 5 48 10.957431486455881 5 49 12.634376890552975 5 50 11.575705614424223 5 51 13.270919465737638 5 52 12.023207705023122 5 53 12.272240900998181 5 54 12.407594408422394 5 55 10.946443847715187 5 56 12.420574374070586 6 1 1.4538386641445342 6 2 2.070236340377541 6 3 2.7729272049684965 6 4 2.43799474580144 6 5 1.4986582932760228 6 6 3.2284090422285487 6 7 2.145294088849873 6 8 2.008756884838604 6 9 2.309009393465942 6 10 3.2425095097895777 6 11 4.2650597251603415 6 12 5.769215879676466 6 13 6.166042263830775 6 14 5.302212848850374 6 15 5.628319771277059 6 16 7.096397126871942 6 17 8.481189259924452 6 18 9.399372386934964 6 19 8.097718202876543 6 20 6.7377894145362625 6 21 6.831572341583694 6 22 7.625427848022184 6 23 6.727277256413554 6 24 8.216711306351549 6 25 7.542741962142266 6 26 6.430770013630479 6 27 5.757624619217175 6 28 5.5244367497151785 6 29 7.031392467959527 6 30 6.9980070271647286 6 31 5.373627292038734 6 32 5.409090398386496 6 33 5.5143912706564 6 34 4.879307166760185 6 35 5.401214794748686 6 36 5.140087825558808 6 37 6.611915234499314 6 38 6.109995216430686 6 39 7.919788606002161 6 40 4.6173571568513765 6 41 8.991264559490883 6 42 10.830382621709116 6 43 11.121246609397218 6 44 12.566685585919199 6 45 10.667776292984442 6 46 12.92155401841918 6 47 14.428565664198565 6 48 12.720909747325443 6 49 14.215246455770366 6 50 13.85222735355466 6 51 11.797875987476768 6 52 11.054512052849208 6 53 12.133110466215573 6 54 11.845855277987614 6 55 11.184704717280404 6 56 9.949270026244502 7 1 1.5790560554488877 7 2 2.3658885142905888 7 3 3.145101118011972 7 4 2.846690397975351 7 5 2.634310467189067 7 6 3.35188730309811 7 7 2.5174680018933486 7 8 2.6574525370125173 7 9 2.7977050456398587 7 10 3.880770379354793 7 11 5.0754945077690365 7 12 6.400520227502554 7 13 7.05473791600469 7 14 5.709169370589507 7 15 7.565711075624886 7 16 8.120744952958894 7 17 8.7768414338375 7 18 9.535024560848008 7 19 7.815979072441763 7 20 7.07344158844931 7 21 6.953311472018477 7 22 8.157601761065663 7 23 7.44379899554399 7 24 8.43758087156894 7 25 7.789698483881395 7 26 6.731639578847869 7 27 6.559363749651958 7 28 5.594001967106486 7 29 7.116609859263882 7 30 6.91800702716473 7 31 6.124931639864819 7 32 6.711699094038671 7 33 5.763086922830315 7 34 6.2619158624123585 7 35 7.241214794748689 7 36 7.136609564689245 7 37 6.582350017108013 7 38 7.553473477300248 7 39 8.133701649480418 7 40 8.198226722068767 7 41 12.424308037751754 7 42 10.715600013013468 7 43 10.84472487026678 7 44 13.262337759832246 7 45 11.664298032114878 7 46 12.591119235810481 7 47 12.725956968546392 7 48 13.261779312542835 7 49 13.76481167316167 7 50 12.575705614424223 7 51 13.183962943998512 7 52 10.588425096327466 7 53 12.924414814041658 7 54 11.451072669291964 7 55 12.511661239019539 7 56 12.507530895809719 8 1 1.4683841186899897 8 2 2.1247817949229955 8 3 2.9474726595139487 8 4 2.4652674730741637 8 5 2.1204764750942005 8 6 2.94477267859218 8 7 1.9562031797589654 8 8 2.3214841575658767 8 9 2.461736666193218 8 10 3.447964055244121 8 11 4.672332452433068 8 12 5.2364886069491945 8 13 5.491496809285323 8 14 3.5767583033958275 8 15 6.8028652258225115 8 16 7.134578945053761 8 17 7.763007441742634 8 18 7.566645114207688 8 19 7.110445475603818 8 20 7.150516687263536 8 21 6.158845068856422 8 22 7.869064211658554 8 23 6.896368165504459 8 24 6.585802215442456 8 25 6.5209237803240825 8 26 6.198042740903205 8 27 6.083079164671723 8 28 4.997164022442455 8 29 5.369574286141351 8 30 4.781643390801097 8 31 4.3245363829478265 8 32 3.474544943841046 8 33 3.3362094524745807 8 34 4.43385262130564 8 35 4.513942067475963 8 36 4.907360552831534 8 37 4.679187961772044 8 38 6.328177034612505 8 39 8.005243151456707 8 40 -2.10809738860317 8 41 9.193082741309066 8 42 8.195837167163663 8 43 9.028519336669945 8 44 7.612140131373746 8 45 7.116867202075348 8 46 11.35791765478281 8 47 11.05402020965311 8 48 10.546364292779991 8 49 11.577064637588546 8 50 10.304954626281933 8 51 9.85787598747677 8 52 10.050875689212841 8 53 11.169474102579205 8 54 11.43130982344216 8 55 12.69743199000768 8 56 10.165633662608137 9 1 1.1090767593826314 9 2 1.3845220546632575 9 3 2.3024510144923056 9 4 1.7141852219919116 9 5 1.51008686470459 9 6 2.9750757088952113 9 7 1.6748178983736821 9 8 1.7630425991243186 9 9 1.950914155370704 9 10 2.878699985980049 9 11 4.7069644870651 9 12 5.294930165390753 9 13 4.684137501926017 9 14 5.241260467897995 9 15 5.776891199848489 9 16 6.38782569830051 9 17 7.795474974210169 9 18 7.982229529792107 9 19 6.290099155257494 9 20 5.8539798907267375 9 21 7.09823900825036 9 22 6.981618324212661 9 23 7.106324875461173 9 24 6.938616068256309 9 25 6.178932438332737 9 26 5.470770013630478 9 27 4.418577000169556 9 28 3.4387224640008967 9 29 4.6466305631976255 9 30 4.7275308366885405 9 31 3.971722530133974 9 32 4.595757065053164 9 33 3.10677222303735 9 34 2.2412119286649457 9 35 3.1935957471296383 9 36 3.799135444606428 9 37 3.5947723773564633 9 38 4.557614264049736 9 39 6.5788362250497805 9 40 2.175452394946614 9 41 8.225550273776602 9 42 9.436096907423401 9 43 9.420294228444838 9 44 10.138114157347768 9 45 9.517300102508251 9 46 10.959649256514417 9 47 12.556184711817615 9 48 11.09614784256354 9 49 11.323817884341796 9 50 9.436989258316565 9 51 10.31025693985772 9 52 9.795464433801587 9 53 10.675967609072718 9 54 11.266807658939992 9 55 9.472323764899453 9 56 11.83879383576831 10 1 1.1090767593826314 10 2 2.003569673710878 10 3 2.921498633539926 10 4 2.523709031515722 10 5 2.1291344837522104 10 6 3.451266185085693 10 7 1.912913136468923 10 8 1.0011378372195523 10 9 2.2366284410849957 10 10 3.0691761764562386 10 11 5.135535915636531 10 12 5.056834927295519 10 13 6.1603279781164915 10 14 4.193641420278944 10 15 6.205462628419912 10 16 7.435444745919561 10 17 7.985951164686359 10 18 9.696515244077816 10 19 7.290099155257494 10 20 6.377789414536263 10 21 6.28871519872655 10 22 7.600665943260282 10 23 6.534896304032603 10 24 7.748139877780119 10 25 6.036075295475598 10 26 5.518389061249529 10 27 6.037624619217176 10 28 5.010151035429466 10 29 6.884725801292866 10 30 6.537054646212351 10 31 4.352674911086353 10 32 4.7386142079103095 10 33 4.4401055563706855 10 34 4.717402404855427 10 35 4.431690985224879 10 36 6.180087825558807 10 37 6.213819996404077 10 38 7.8909475973830645 10 39 9.007407653621211 10 40 10.365928585422807 10 41 11.892216940443266 10 42 10.769430240756737 10 43 11.134579942730554 10 44 12.61430463353825 10 45 12.041109626317777 10 46 13.388220685085841 10 47 13.794279949912848 10 48 13.953290699706393 10 49 13.228579789103701 10 50 11.675084496411806 10 51 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2.879307166760185 115 35 3.707881461415358 115 36 4.646754492225476 115 37 5.051915234499319 115 38 5.176661883097349 115 39 7.359788606002159 115 40 7.470690490184708 115 41 8.911264559490885 115 42 7.483715955042452 115 43 4.801246609397218 115 44 -3.766647747414133 115 45 6.641109626317775 115 46 -0.47844598158082263 115 47 -8.558101002468103 115 48 1.4009097473254428 115 49 -5.638086877562969 115 50 -1.001105979778675 115 51 2.8912093208101055 115 52 0.9478453861825429 115 53 3.133110466215573 115 54 0.4858552779876142 115 55 2.2247047172804066 115 56 5.0292700262445 116 1 1.3471719974778722 116 2 2.003569673710878 116 3 2.826260538301831 116 4 2.126883634690323 116 5 1.8275471821649063 116 6 2.4750757088952113 116 7 2.0875163110720933 116 8 1.8265346626163819 116 9 2.411231615688166 116 10 2.5691761764562386 116 11 1.333948614049227 116 12 2.3425492130098036 116 13 3.890486708275219 116 14 3.45554618218371 116 15 -3.3183468953896096 116 16 4.2052860157608265 116 17 2.5256337043688966 116 18 5.228261275823847 116 19 2.3377182028765446 116 20 3.43334497009182 116 21 5.653794563805917 116 22 5.505427848022187 116 23 5.709499478635774 116 24 5.6767113063515495 116 25 5.702741962142262 116 26 5.970770013630478 116 27 5.870957952550512 116 28 5.446658971937403 116 29 4.495836912403973 116 30 4.862451471609173 116 31 5.011405069816512 116 32 5.040201509497614 116 33 4.5432801595452865 116 34 4.034862722315744 116 35 4.518992572526468 116 36 3.7356433811143646 116 37 6.0074707900548745 116 38 6.7877729942084635 116 39 8.45978860600216 116 40 10.548468267962491 116 41 9.122375670602 116 42 9.483715955042452 116 43 3.690135498286107 116 44 4.844463363696978 116 45 9.374442959651105 116 46 1.8882206850858445 116 47 -4.602545446912547 116 48 2.112020858436555 116 49 1.617468677992587 116 50 3.03222735355466 116 51 5.8578759874767705 116 52 6.136734275071429 116 53 6.366443799548904 116 54 6.052521944654281 116 55 6.2024824950581845 116 56 7.973714470688943 117 1 -0.2004470501411788 117 2 0.09880776894897281 117 3 0.7786414906827801 117 4 0.8094233172300065 117 5 0.51008686470459 117 6 1.2369804707999776 117 7 0.5081512317070178 117 8 0.7630425991243186 117 9 0.4747236791802294 117 10 0.8310809383610049 117 11 -2.6263688462682317 117 12 -11.133641263180674 117 13 -4.982529164740651 117 14 -3.306358579721053 117 15 -10.580251657294369 117 16 -6.3978885874137745 117 17 0.3669035456387384 117 18 -3.803484755922181 117 19 -1.7337103685520283 117 20 -1.3364962997494523 117 21 1.7887151987265497 117 22 2.7911421337364715 117 23 1.0587058278421217 117 24 2.9624255920658342 117 25 2.7027419621422624 117 26 3.8993414422019086 117 27 3.2519103335028916 117 28 1.2244367497151813 117 29 1.1228210393881 117 30 2.060864170021876 117 31 0.5907701491815871 117 32 1.0005189698150687 117 33 0.8686769849421161 117 34 1.3364500239030406 117 35 2.3840719376058352 117 36 2.6800878255588074 117 37 1.3804866630707444 117 38 3.7242809307164 117 39 5.316931463145014 117 40 8.008785728279946 117 41 5.2255502737766015 117 42 3.7218111931376896 117 43 -4.341610533459924 117 44 -4.790457271223659 117 45 2.0649191501272988 117 46 -1.8498745530093963 117 47 -3.0628629072300093 117 48 1.0009097473254442 117 49 -3.461896401372492 117 50 1.460798782126087 117 51 5.8578759874767705 117 52 5.05736919570635 117 53 5.390253323358429 117 54 3.2429981351304704 117 55 4.996133288708979 117 56 5.886412883387358 118 1 -0.2938536435477701 118 2 0.3625440326852356 118 3 1.1852348972761888 118 4 0.9303024381091305 118 5 0.630965985583714 118 6 2.0007167345362404 118 7 0.5576017811575653 118 8 0.6641415002232165 118 9 0.7274709319274848 118 10 2.0948172020972677 118 11 -0.8241710440704253 118 12 -10.067707197246609 118 13 -7.477034659246147 118 14 -8.30086407422655 118 15 -7.959372536415248 118 16 -11.7220644115896 118 17 -1.8418876631524697 118 18 -6.929858382295809 118 19 -1.0468971817388386 118 20 -0.08374904700219687 118 21 3.3546492646606154 118 22 5.274658617252953 118 23 2.256508025644319 118 24 4.869018998659243 118 25 4.2412035006038025 118 26 4.778462321322785 118 27 4.576086157678716 118 28 4.185975211253641 118 29 5.705238621805684 118 30 5.9949301040878105 118 31 4.5028580612694995 118 32 4.0060134753095795 118 33 4.308237424502554 118 34 3.71007639752942 118 35 3.3181378716717695 118 36 6.103164748635727 118 37 6.528838311422398 118 38 6.202302908738378 118 39 7.4982501444637 118 40 8.62966484915907 118 41 3.972803021029346 118 42 4.150382621709117 118 43 -4.8910610829104755 118 44 -2.817929798696184 118 45 3.707776292984441 118 46 -2.58613828927313 118 47 -4.568357412724513 118 48 -1.7683210219053258 118 49 -3.412445851921941 118 50 0.6860735074008133 118 51 4.742491372092154 118 52 3.1068197451569013 118 53 1.8408027739078783 118 54 5.116624508756843 118 55 5.117012409588099 118 56 7.79850079547527 119 1 -0.8433041929983176 119 2 -0.1869065167653119 119 3 0.4929272049684954 119 4 -0.2620052541985629 119 5 0.08151543613316647 119 6 0.16555189937140113 119 7 -0.3489916254358434 119 8 -0.5226716865899661 119 9 0.046152250608798795 119 10 1.4025095097895743 119 11 -0.5549402748396588 119 12 -7.84792697746639 119 13 -3.053957736169224 119 14 -10.306358579721053 119 15 -7.794537371580084 119 16 -13.969317158842344 119 17 -5.061667882932689 119 18 -8.803484755922181 119 19 -2.3051389399805977 119 20 -2.550782014035164 119 21 3.360143770155126 119 22 4.005427848022187 119 23 3.415848684984983 119 24 0.6767113063515495 119 25 2.7741705335708318 119 26 2.8993414422019086 119 27 4.037624619217176 119 28 2.2958653211437507 119 29 2.6942496108166765 119 30 3.9894355985932997 119 31 0.019341577753017702 119 32 2.1433761126722146 119 33 0.7258198420849702 119 34 0.9793071667601865 119 35 0.24121479474868934 119 36 1.1800878255588074 119 37 -0.19094190835782499 119 38 0.7957095021449696 119 39 1.1740743202878754 119 40 4.080214299708519 119 41 2.582693130919459 119 42 1.4360969074234013 119 43 -3.4844676763170668 119 44 -1.0761715569379433 119 45 0.9934905786987258 119 46 -1.2070174101522504 119 47 -4.205720050087152 119 48 -6.499090252674556 119 49 -4.461896401372492 119 50 -0.46777264644534 119 51 1.7864474160481976 119 52 2.985940624277781 119 53 2.1045390376441446 119 54 1.2429981351304704 119 55 1.0675618601375483 119 56 3.886412883387358 120 1 0.4513386641445365 120 2 1.1702363403775422 120 3 2.1804272049684954 120 4 1.925494745801437 120 5 1.6261582932760206 120 6 2.870909042228547 120 7 1.490294088849872 120 8 1.6737568848386033 120 9 1.0015093934659447 120 10 2.8400095097895743 120 11 0.5700597251603412 120 12 -3.678284120323532 120 13 -3.303957736169224 120 14 -4.690287151149626 120 15 0.5983197712770583 120 16 -5.496102873128059 120 17 1.0186892599244537 120 18 -0.5981276130650386 120 19 -0.8497817971234554 120 20 -0.747210585463737 120 21 2.2440723415836956 120 22 4.567927848022187 120 23 4.299777256413552 120 24 5.1767113063515495 120 25 4.015241962142262 120 26 5.345770013630478 120 27 4.350124619217176 120 28 3.3494367497151813 120 29 4.676392467959531 120 30 4.79300702716473 120 31 2.421127292038733 120 32 1.1790903983864993 120 33 2.841891270656401 120 34 3.2293071667601865 120 35 3.6787147947486893 120 36 5.492587825558807 120 37 4.014415234499317 120 38 4.822495216430685 120 39 7.70978860600216 120 40 7.062357156851377 120 41 -5.863735440509114 120 42 -0.8496173782908834 120 43 -7.011253390602782 120 44 -14.183314414080801 120 45 -0.9172237070155589 120 46 -7.840945981580823 120 47 -9.741434335801436 120 48 -2.8115902526745558 120 49 -14.604753544229634 120 50 -2.28027264644534 120 51 1.7953759874767705 120 52 3.789512052849208 120 53 4.033110466215572 120 54 1.5108552779876128 120 55 3.237204717280406 120 56 9.3417700262445 >>END_MODULE >>Per sequence quality scores pass #Quality Count 4 68.0 5 29.0 6 90.0 7 63.0 8 135.0 9 167.0 10 68.0 11 96.0 12 82.0 13 42.0 14 36.0 15 40.0 16 63.0 17 75.0 18 77.0 19 120.0 20 163.0 21 229.0 22 317.0 23 353.0 24 423.0 25 449.0 26 524.0 27 540.0 28 598.0 29 591.0 30 660.0 31 669.0 32 886.0 33 968.0 34 1033.0 35 1150.0 36 1190.0 37 1355.0 38 1430.0 39 1703.0 40 2.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 0.0 0.0 0.0 100.0 2 0.0 0.0 100.0 0.0 3 0.0 0.0 100.0 0.0 4 0.0 0.0 0.0 100.0 5 0.0 0.0 100.0 0.0 6 100.0 0.0 0.0 0.0 7 99.22348944430964 0.6551807813637467 0.06066488716330988 0.06066488716330988 8 98.61077408396021 0.8614413977190002 0.37612230041252126 0.1516622179082747 9 94.95874787672895 1.9170104343605923 2.27493326862412 0.8493084202863382 10 47.50060664887164 19.000242659548654 25.74011162339238 7.759039068187333 11 32.297985925746175 27.572191215724338 12.181509342392623 27.948313516136857 12 31.22421742295559 27.208201892744476 16.015530211113806 25.55205047318612 13 19.564426110167435 16.57364717301626 17.695947585537493 46.16597913127882 14 17.853676292162096 33.499150691579715 27.784518320795925 20.862654695462265 15 41.08787121165925 17.141289102994083 21.476919324349044 20.29392036099762 16 31.30350312670755 32.244551029081414 19.89557403922045 16.55637180499059 17 24.723874256584537 20.37261803616944 37.92329166160942 16.980216045636606 18 25.81290948798835 19.16403785488959 22.822130550837176 32.200922106284885 19 26.544483553829345 25.76769025367156 16.682849860419953 31.00497633207914 20 24.348301634562798 28.89347997812481 29.877863523120862 16.88035486419153 21 21.051992962446157 23.533337377904505 25.438330401019233 29.9763392586301 22 19.127638922591604 20.5168648386314 37.22397476340694 23.131521475370057 23 22.737199708808543 20.832322251880612 32.62557631642805 23.804901722882796 24 23.818479645695565 21.695079779166417 24.534368743553962 29.952071831584053 25 26.90044288054359 20.83358611903173 29.63052842322393 22.635442577200752 26 33.276678402330944 22.03472137914289 19.734126502367367 24.954473716158795 27 18.80009715812485 34.54578576633471 18.01675977653631 28.63735729900413 28 26.4680902693521 38.425139529240475 15.48167920407668 19.625090997330744 29 36.934856587263006 31.137578998541564 15.678172095284395 16.249392318911035 30 28.933794526366892 22.835123490503065 32.35026397232842 15.880818010801626 31 33.2261391905831 31.80632243189127 19.780353133911778 15.187185243613857 32 39.39559439286364 28.18739001152983 16.94277565386249 15.474239941744038 33 33.197692074096565 27.409656847859093 19.4959003947768 19.896750683267538 34 18.9619545399295 26.133462987723348 38.73830071715085 16.166281755196305 35 18.182923123670616 28.234579155271955 39.647523549073235 13.9349741719842 36 25.072833211944644 34.207331876669095 24.81184753580966 15.907987375576596 37 29.424630901026795 25.8824958989003 29.685886141320854 15.006987058752049 38 32.441471571906355 26.573426573426573 24.250532076619034 16.73456977804804 39 29.422130400437503 24.676429482894815 17.712827368293127 28.188612748374553 40 22.131147540983605 17.733056031318817 28.42549547345241 31.71030095424517 41 17.800822237221574 18.733509234828496 24.611891759219485 38.85377676873044 42 21.730932976523537 29.491546040627657 20.1252888942951 28.652232088553703 43 15.63926245968478 25.394024219558204 30.931661899835696 28.035051420921313 44 18.287629494210847 27.544180377818407 26.15478366849482 28.01340645947593 45 24.835766423357665 24.750608272506085 29.80535279805353 20.608272506082727 46 16.564230511775087 31.357634029087812 20.397979675044116 31.680155784092985 47 14.914902702372965 29.79320441651925 21.808088818398097 33.483804062709694 48 25.182127246236035 26.772705196697427 23.87081107333657 24.174356483729966 49 18.577339661677012 29.645856151880253 21.759766338079594 30.01703784836315 50 20.89869877173781 28.365560014593218 23.397786695853096 27.33795451781588 51 15.845563607575667 32.7690152853054 25.31514524085013 26.0702758662688 52 16.225910389689343 34.184448902668855 29.868077086752994 19.72156362088881 53 16.532797858099062 32.54837531945965 30.917609833272486 20.001216989168796 54 16.516607859836963 27.637182138946343 31.293344689134933 24.552865312081764 55 15.743050063872499 26.005231461767746 29.411764705882355 28.8399537684774 56 20.940274905729233 24.461744313343875 28.536674370514536 26.06130641041236 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 1.0 7 2.0 8 6.0 9 10.0 10 38.5 11 67.0 12 92.5 13 120.0 14 122.0 15 111.5 16 101.0 17 91.0 18 81.0 19 73.5 20 66.0 21 66.5 22 64.5 23 62.0 24 67.0 25 72.0 26 78.5 27 85.0 28 101.5 29 118.0 30 118.0 31 124.0 32 130.0 33 150.5 34 171.0 35 227.5 36 284.0 37 260.0 38 237.0 39 238.0 40 264.5 41 291.0 42 353.0 43 415.0 44 555.0 45 695.0 46 1214.5 47 1673.0 48 1612.0 49 1264.5 50 917.0 51 856.0 52 795.0 53 822.5 54 891.0 55 932.0 56 917.5 57 903.0 58 972.5 59 1042.0 60 1078.0 61 1114.0 62 1060.5 63 842.0 64 677.0 65 575.5 66 474.0 67 385.0 68 296.0 69 244.5 70 193.0 71 173.0 72 117.5 73 82.0 74 77.5 75 73.0 76 50.5 77 28.0 78 26.0 79 23.5 80 23.0 81 14.5 82 6.0 83 3.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.515651540888134 16 0.07886435331230283 17 0.03639893229798593 18 0.0 19 0.03639893229798593 20 0.16379519534093667 21 0.006066488716330988 22 0.0 23 0.0 24 0.006066488716330988 25 0.006066488716330988 26 0.06066488716330988 27 0.0970638194612958 28 0.0 29 0.16986168405726765 30 0.03033244358165494 31 0.018199466148992965 32 0.03033244358165494 33 0.11526328561028876 34 0.18199466148992963 35 0.17592817277359865 36 0.0485319097306479 37 0.1516622179082747 38 0.23659305993690852 39 0.16379519534093667 40 0.8250424654210143 41 1.1344333899538948 42 0.25479252608590147 43 0.30939092453288036 44 0.44892016500849313 45 0.2669255035185635 46 0.30939092453288036 47 0.5520504731861199 48 0.07279786459597186 49 0.3033244358165494 50 0.23052657122057751 51 0.38218878912885224 52 0.21232710507158456 53 0.3033244358165494 54 0.27905848095122543 55 0.27299199223489445 56 0.25479252608590147 >>END_MODULE >>Sequence Length Distribution pass #Length Count 56 16484.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 72.43994176170833 #Duplication Level Percentage of deduplicated Percentage of total 1 88.79490829913742 64.32297985925746 2 6.716355414119421 9.730647900994905 3 2.0768779834184743 4.513467604950255 4 0.8207017837702035 2.378063576801747 5 0.5024704798593083 1.8199466148992962 6 0.2596097479273093 1.1283669012375637 7 0.14236663596013735 0.7219121572433875 8 0.1339921279624822 0.7765105556903664 9 0.1088686039695168 0.7097791798107256 >10 0.3768528598944812 5.708565882067459 >50 0.025123523992965417 1.2132977432661975 >100 0.04187253998827569 6.976462023780636 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source CTTCTGGGGTCACAGATCCTCTTCTGAGATGAGTTTTTGTTCAATCCGCCCAATCT 346 2.0990050958505218 No Hit CTTCTGGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAG 343 2.080805629701529 No Hit CTTCTGGGGTCACAGATCCTCTTCTGAAGGAGATTGGGCGGATTGAACAAAAACTC 228 1.383159427323465 No Hit CTTCTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC 130 0.7886435331230284 No Hit CTTCTGGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTCGTCTCAAAGATTAAG 103 0.6248483377820917 No Hit CTTCTGGGGTCACAGATCCTCTTCTGAAGGAGATTGGGCGCATTGAACAAAAACTC 75 0.45498665372482405 No Hit CTTCTGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTC 73 0.4428536762921621 No Hit CTTCTGGGGTCACAGATCCTCTTCTGAGATGAGTTTTTGTCCAATCCGCCCAATCT 52 0.31545741324921134 No Hit CTTCTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCCCCCGACTGCTCTTCC 47 0.2851249696675564 No Hit CTTCTGGGGTCACAGATCCTCTTCTGAGATGAGTTTTTGTTCAATCCGCCCAAAAA 44 0.2669255035185635 No Hit CTTCTGGGGTACCTGGTTGATCCTGCCAGTAGCATATGCCTGTCTCAAAGATTAAG 44 0.2669255035185635 No Hit CTTCTGGGTCACAGATCCTCTTCTGAGATGAGTTTTTGTTCAATCCGCCCAATCTC 43 0.26085901480223245 No Hit CTTCTGGGGACTAATCCTAGCCCTAGCCCTACACAAATATAATTATACTATTATAT 41 0.2487260373695705 No Hit CTTCTGGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGC 41 0.2487260373695705 No Hit CTTCTGGGGTCACAGATCCTCTTCTGAAGGAGATTGGGCCGATTGAACAAAAACTC 39 0.23659305993690852 No Hit CTTCTGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 38 0.23052657122057751 No Hit CTTCTGGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCCAAGATTAAG 33 0.20019412763892258 No Hit CTTCTGGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTT 26 0.15772870662460567 No Hit CTTCTGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 26 0.15772870662460567 No Hit CTTCTGGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCCACGATTAAG 25 0.1516622179082747 No Hit CTTCTGGGGGGGCGCTGACCCCCCTTCCCGGGGGGGGATGCGTGCATTTATCAGAT 23 0.13952924047561271 No Hit CTTCTGGGGTCACAGATCCTCTTCTGAGATGAGTTTTTGCTCAATCCGCCCAATCT 23 0.13952924047561271 No Hit CTTCTGGGGACTAATCCTAGCCCTAGCCCTACACAAATATCATTATACTATTATAT 23 0.13952924047561271 No Hit CTTCTGGGGGGGCTGGAGAGATGGCTCAGTGGTTAAGAGCACTGACTGCTCTTCCA 23 0.13952924047561271 No Hit CTTCTGGGGTACCTGGTTGATCCTGCCAGTAGCATATGCCGTCTTCTGCTTGGATC 22 0.13346275175928174 No Hit CTTCTGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACAAAAAAAAAAAAAAA 22 0.13346275175928174 No Hit CTTCTGGGGTCACAGATCCTCTTCTGAAGGAGATTGGGCGGATTGACCAAAAACTC 19 0.11526328561028876 No Hit CTTCTGGGGGCAAGCTCCGTTGGGCGCCTTGGATCCATTTCCATCTGGTTCTGCTG 18 0.10919679689395778 No Hit CTTCTGGGGGATCCCCTCGACCACTCTTTTGGCGCTTCGCTGTCGACAGTGCGCTT 17 0.10313030817762679 No Hit CTTCTGGGGTAAGGGAGAATCTTGCCTGGCTCTCTTCTCTTGGGCCTCTAAGAAAG 17 0.10313030817762679 No Hit CTTCTGGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 17 0.10313030817762679 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0 0.0 0.0 0.0 0.0 11 0.0 0.0 0.0 0.0 0.0 12 0.0 0.0 0.0 0.0 0.0 13 0.0 0.0 0.0 0.0 0.0 14 0.012132977432661975 0.0 0.0 0.0 0.0 15 0.01819946614899296 0.0 0.0 0.0 0.0 16 0.01819946614899296 0.0 0.0 0.0 0.0 17 0.030332443581654937 0.0 0.0 0.0 0.0 18 0.030332443581654937 0.0 0.0 0.0 0.0 19 0.03639893229798592 0.0 0.0 0.0 0.0 20 0.03639893229798592 0.0 0.0 0.0 0.0 21 0.04246542101431691 0.0 0.0 0.0 0.0 22 0.0485319097306479 0.0 0.0 0.0 0.0 23 0.0485319097306479 0.0 0.0 0.0 0.0 24 0.0485319097306479 0.0 0.0 0.0 0.0 25 0.05459839844697889 0.0 0.0 0.0 0.0 26 0.06066488716330987 0.0 0.0 0.0 0.0 27 0.07279786459597185 0.0 0.0 0.0 0.0 28 0.07886435331230283 0.0 0.0 0.0 0.0 29 0.08493084202863382 0.0 0.0 0.0 0.0 30 0.08493084202863382 0.0 0.0 0.0 0.0 31 0.09099733074496481 0.0 0.0 0.0 0.0 32 0.09099733074496481 0.0 0.0 0.0 0.0 33 0.0970638194612958 0.0 0.0 0.0 0.0 34 0.10919679689395778 0.0 0.0 0.0 0.0 35 0.10919679689395778 0.0 0.0 0.0 0.0 36 0.10919679689395778 0.0 0.0 0.0 0.0 37 0.10919679689395778 0.0 0.0 0.0 0.0 38 0.10919679689395778 0.0 0.0 0.0 0.0 39 0.11526328561028877 0.0 0.0 0.0 0.0 40 0.11526328561028877 0.0 0.0 0.0 0.0 41 0.11526328561028877 0.0 0.0 0.0 0.0 42 0.12132977432661975 0.0 0.0 0.0 0.0 43 0.13952924047561271 0.0 0.0 0.0 0.0 44 0.1516622179082747 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CCAATCC 15 0.007700431 50.92835 41 TGTCTCC 20 3.708414E-4 50.92835 41 CGTCTCA 20 3.708414E-4 50.92835 41 CCCTGAC 15 0.00789275 50.613003 40 GACCACC 15 0.00789275 50.613003 38 AGCCCTG 15 0.00789275 50.613003 38 GCCCTGA 15 0.00789275 50.613003 39 GACCAAA 15 0.007990238 50.45679 45 TTGTCCA 15 0.00808862 50.30154 37 TAAGACC 15 0.00808862 50.30154 35 AGCAGTG 30 9.4494317E-7 50.30154 11 AAGACCA 15 0.00808862 50.30154 36 TAGAGCG 15 0.00808862 50.30154 34 GTTGGGG 15 0.00808862 50.30154 13 AGACCAC 15 0.00808862 50.30154 37 AAGCAGT 35 4.6446075E-8 50.30154 10 TATGCTC 20 3.9436133E-4 50.301537 35 AGAGCCC 20 3.9436133E-4 50.301537 36 GGCTCTT 20 3.9436133E-4 50.301537 9 ATGCTCG 20 3.9436133E-4 50.301537 36 >>END_MODULE